RLG00000012254

phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016264
Physical Location & Seq
Forward (+)
16774594 .. 16775019
426 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012254

Sequence Viewer

Length: 426 bp
ATGGTCACGGCGATTGTCTTTGGCTGTCCCTTTGTTGGAAACGAAGCCTTTAAGAAGGCGGAGAACGTTGAGACCATGTATCAGATCTTGCGGGTCACAAATGAACCAGATCCAGTGACTAATTCGAAGCCTTCTCCGGAATATGAACATATTGGAGTGGAGGTGAAGATTAACACAGTCAATTCGCCCTACTTGAAGAATCCAATAGATACCGTACATGAGTTGGAGGTTTACTTGCATGGGGTTGCAGCACACCCAGGGACAACAGTGGCATTGGAAGATATCCGCCGTACAGTGATGGTGAACAAAACATTGGACGGTCTTAAGGATGAAACTAAAGTCCCCCCCATCTGGATTTTAAATAAGTTCATGGTTCAAAATGAGAGAGGCGCCTGGGAGCTGAAGGATGTTCCCGTTGACGATTAG

Protein Analysis

142

Amino Acids

15.86

Weight (kDa)

5.07

Isoelectric Point (pI)

28.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 2 - 42 8.8e-06 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016264)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g22960 FvH4_2g22990
rosa_chinensis RchiOBHm_Chr6g0289981
rosa_laevigata RLG00000012253 RLG00000012254 RLG00000012255
rosa_multiflora Rmu_sc0002902.1_g000066 Rmu_sc0003927.1_g000011
rosa_roxburghii Rroxscaffold_7G00177450
rosa_rugosa Rorug06G0208200
rosa_samantha Rh6AG319600 Rh6BG326700 Rh6CG332500 Rh6DG318400
rosa_wichuraiana Rw6G027580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 389
AccIII TCCGGA 1 cut(s) 136
AciI CCGC 3 cut(s) 59, 91, 286
AclI AACGTT 1 cut(s) 66
AclWI GGATC 1 cut(s) 104
AcuI CTGAAG 1 cut(s) 422
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 2 cut(s) 216, 292
AfiI CCNNNNNNNGG 2 cut(s) 35, 351
AflII CTTAAG 1 cut(s) 323
AgsI TTSAA 2 cut(s) 196, 377
AjnI CCWGG 2 cut(s) 256, 392
AjuI GAANNNNNNNTTGG 2 cut(s) 296, 328
AluBI AGCT 1 cut(s) 400
AluI AGCT 1 cut(s) 400
Alw26I GTCTC 1 cut(s) 65
AlwI GGATC 1 cut(s) 104
Aor13HI TCCGGA 1 cut(s) 136
ApeKI GCWGC 1 cut(s) 248
AspLEI GCGC 1 cut(s) 392
AsuHPI GGTGA 2 cut(s) 175, 313
AsuII TTCGAA 1 cut(s) 125
BanI GGYRCC 1 cut(s) 389
BbvI GCAGC 1 cut(s) 260
BccI CCATC 2 cut(s) 292, 356
BceAI ACGGC 2 cut(s) 24, 273
BciT130I CCWGG 2 cut(s) 258, 394
BcoDI GTCTC 1 cut(s) 65
BfoI RGCGCY 1 cut(s) 393
BfrI CTTAAG 1 cut(s) 323
BglII AGATCT 1 cut(s) 84
BisI GCNGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 250
Bme1390I CCNGG 2 cut(s) 258, 394
BmiI GGNNCC 1 cut(s) 391
BmrFI CCNGG 2 cut(s) 258, 394
Bpu14I TTCGAA 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 390
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 3 cut(s) 256, 257, 393
BsaWI WCCGGW 1 cut(s) 136
BsaXI ACNNNNNCTCC 2 cut(s) 147, 177
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 351
Bse1I ACTGG 1 cut(s) 113
BseAI TCCGGA 1 cut(s) 136
BseBI CCWGG 2 cut(s) 258, 394
BseDI CCNNGG 3 cut(s) 256, 257, 393
BseGI GGATG 2 cut(s) 334, 412
BseLI CCNNNNNNNGG 2 cut(s) 35, 351
BseNI ACTGG 1 cut(s) 113
BseXI GCAGC 1 cut(s) 260
BshNI GGYRCC 1 cut(s) 389
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 3 cut(s) 12, 274, 326
BslI CCNNNNNNNGG 2 cut(s) 35, 351
BsmAI GTCTC 1 cut(s) 65
BsmFI GGGAC 3 cut(s) 12, 274, 326
Bso31I GGTCTC 1 cut(s) 65
Bsp119I TTCGAA 1 cut(s) 125
Bsp13I TCCGGA 1 cut(s) 136
Bsp143I GATC 2 cut(s) 84, 109
BspACI CCGC 3 cut(s) 59, 91, 286
BspEI TCCGGA 1 cut(s) 136
BspLI GGNNCC 1 cut(s) 391
BspPI GGATC 1 cut(s) 104
BspT104I TTCGAA 1 cut(s) 125
BspT107I GGYRCC 1 cut(s) 389
BspTI CTTAAG 1 cut(s) 323
BspTNI GGTCTC 1 cut(s) 65
BsrI ACTGG 1 cut(s) 113
BssECI CCNNGG 3 cut(s) 256, 257, 393
BssMI GATC 2 cut(s) 84, 109
BssNI GRCGYC 1 cut(s) 390
Bst2UI CCWGG 2 cut(s) 258, 394
Bst4CI ACNGT 5 cut(s) 178, 214, 268, 295, 320
BstACI GRCGYC 1 cut(s) 390
BstAFI CTTAAG 1 cut(s) 323
BstBI TTCGAA 1 cut(s) 125
BstF5I GGATG 2 cut(s) 334, 412
BstH2I RGCGCY 1 cut(s) 393
BstHHI GCGC 1 cut(s) 392
BstKTI GATC 2 cut(s) 87, 112
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 2 cut(s) 84, 109
BstNI CCWGG 2 cut(s) 258, 394
BstSCI CCNGG 2 cut(s) 256, 392
BstV1I GCAGC 1 cut(s) 260
BstX2I RGATCY 2 cut(s) 84, 109
BstYI RGATCY 2 cut(s) 84, 109
BtsCI GGATG 2 cut(s) 334, 412
BtsIMutI CAGTG 3 cut(s) 120, 273, 300
CfoI GCGC 1 cut(s) 392
Csp6I GTAC 2 cut(s) 215, 291
CviAII CATG 4 cut(s) 76, 218, 239, 370
CviJI RGCY 4 cut(s) 24, 47, 130, 400
CviKI_1 RGCY 4 cut(s) 24, 47, 130, 400
CviQI GTAC 2 cut(s) 215, 291
DinI GGCGCC 1 cut(s) 391
DpnI GATC 2 cut(s) 86, 111
DpnII GATC 2 cut(s) 84, 109
DraI TTTAAA 1 cut(s) 360
EciI GGCGGA 2 cut(s) 74, 275
Eco31I GGTCTC 1 cut(s) 65
Eco32I GATATC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 422
EcoRII CCWGG 2 cut(s) 256, 392
EcoRV GATATC 1 cut(s) 283
EgeI GGCGCC 1 cut(s) 391
EheI GGCGCC 1 cut(s) 391
FaeI CATG 4 cut(s) 79, 221, 242, 373
FaiI YATR 6 cut(s) 77, 144, 150, 219, 240, 371
FaqI GGGAC 3 cut(s) 12, 274, 326
FatI CATG 4 cut(s) 75, 217, 238, 369
FauI CCCGC 1 cut(s) 84
Fnu4HI GCNGC 1 cut(s) 249
FokI GGATG 2 cut(s) 341, 419
Fsp4HI GCNGC 1 cut(s) 249
GlaI GCGC 1 cut(s) 391
GluI GCNGC 1 cut(s) 249
HaeII RGCGCY 1 cut(s) 393
HapII CCGG 1 cut(s) 137
HhaI GCGC 1 cut(s) 392
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 4 cut(s) 79, 221, 242, 373
Hin6I GCGC 1 cut(s) 390
HinP1I GCGC 1 cut(s) 390
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 1 cut(s) 199
HpaII CCGG 1 cut(s) 137
HphI GGTGA 2 cut(s) 175, 313
Hpy166II GTNNAC 3 cut(s) 232, 304, 418
Hpy188I TCNGA 1 cut(s) 84
Hpy188III TCNNGA 2 cut(s) 137, 352
Hpy8I GTNNAC 3 cut(s) 232, 304, 418
HpyAV CCTTC 3 cut(s) 49, 141, 397
HpyCH4III ACNGT 5 cut(s) 178, 214, 268, 295, 320
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 2 cut(s) 238, 248
HpySE526I ACGT 1 cut(s) 66
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 4 cut(s) 79, 221, 242, 373
HspAI GCGC 1 cut(s) 390
KasI GGCGCC 1 cut(s) 389
Kpn2I TCCGGA 1 cut(s) 136
Kzo9I GATC 2 cut(s) 84, 109
LmnI GCTCC 1 cut(s) 397
LpnPI CCDG 8 cut(s) 120, 126, 150, 243, 270, 337, 379, 406
Lsp1109I GCAGC 1 cut(s) 260
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 3 cut(s) 4, 94, 115
MalI GATC 2 cut(s) 86, 111
MboI GATC 2 cut(s) 84, 109
MboII GAAGA 3 cut(s) 178, 208, 290
MflI RGATCY 2 cut(s) 84, 109
MluCI AATT 2 cut(s) 121, 181
Mly113I GGCGCC 1 cut(s) 390
MmeI TCCRAC 2 cut(s) 16, 204
MnlI CCTC 3 cut(s) 154, 220, 380
MroI TCCGGA 1 cut(s) 136
MseI TTAA 4 cut(s) 51, 171, 324, 359
MspCI CTTAAG 1 cut(s) 323
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 2 cut(s) 258, 394
MvaI CCWGG 2 cut(s) 258, 394
NarI GGCGCC 1 cut(s) 390
NdeII GATC 2 cut(s) 84, 109
NlaIII CATG 4 cut(s) 79, 221, 242, 373
NlaIV GGNNCC 1 cut(s) 391
NmuCI GTSAC 3 cut(s) 4, 94, 115
NspV TTCGAA 1 cut(s) 125
PasI CCCWGGG 1 cut(s) 257
PfeI GAWTC 1 cut(s) 199
PkrI GCNGC 1 cut(s) 250
PluTI GGCGCC 1 cut(s) 393
Psp1406I AACGTT 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 256, 392
PspGI CCWGG 2 cut(s) 256, 392
PspN4I GGNNCC 1 cut(s) 391
PsuI RGATCY 2 cut(s) 84, 109
RsaI GTAC 2 cut(s) 216, 292
RsaNI GTAC 2 cut(s) 215, 291
SaqAI TTAA 4 cut(s) 51, 171, 324, 359
SatI GCNGC 1 cut(s) 249
Sau3AI GATC 2 cut(s) 84, 109
ScrFI CCNGG 2 cut(s) 258, 394
SetI ASST 4 cut(s) 69, 165, 231, 402
SfoI GGCGCC 1 cut(s) 391
SfuI TTCGAA 1 cut(s) 125
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
Sse9I AATT 2 cut(s) 121, 181
SsiI CCGC 3 cut(s) 59, 91, 286
SspDI GGCGCC 1 cut(s) 389
StyD4I CCNGG 2 cut(s) 256, 392
TaaI ACNGT 5 cut(s) 178, 214, 268, 295, 320
TaiI ACGT 1 cut(s) 69
TaqI TCGA 1 cut(s) 125
TasI AATT 2 cut(s) 121, 181
TfiI GAWTC 1 cut(s) 199
Tru1I TTAA 4 cut(s) 51, 171, 324, 359
Tru9I TTAA 4 cut(s) 51, 171, 324, 359
TscAI CASTG 3 cut(s) 120, 273, 300
TseFI GTSAC 3 cut(s) 4, 94, 115
TseI GCWGC 1 cut(s) 248
Tsp45I GTSAC 3 cut(s) 4, 94, 115
TspDTI ATGAA 4 cut(s) 117, 159, 345, 358
TspRI CASTG 3 cut(s) 120, 273, 300
Vha464I CTTAAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.