Rh6BG326700

phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0016264
Physical Location & Seq
Reverse (-)
55866632 .. 55868349
1718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG326700.1

Sequence Viewer

Length: 618 bp
ATGGTGCACGGTGGTTGGTATGATTATTACACTAATACTGACGTTAGGACCCCCAAGAACCAACGAACTAGTTGCCAAGATCAGGTTGGAGAGGCCGTTAGAAAACTGATAGATCAATACGGTAACGAGGGTCAACAACTCAGCATTACTATAACTGGCCGCGGCACTGGTGCTGCATTTGCAATTTTAAATGGGATGGATATTGTTTGCAACAAACTCAATTATCCTACAAAACAACCCAAGAGCCCATGTCTGGTCACGGCGATTGTCTTTGGCTGTCCCTTTGTTGGAAACGAAGCCTTTAAGAATGCGGAGAACGATGAGACCATGTTTCAGATCTTGCGGGTCACAAATGAACCAGATCCAGTGACTAATTCGGAGCCTTCTCCGGAATATGAACATATTGGAGTAGAGGTGAAAATTAACACAGTCAATTCGCCCTACTTGAAGAATCCAATAGATACCGTACATGAGTTGGAGGTTTACTTGCATGGGGTTGCAGCGCACCCAGGGACAACTGTGGCATTGGAAGATATCCGCCGTACAGTGATGGTGAACAAAACATTGGACGGTCTTAAGGAAGAAACTATAGTCCCCCCCCATCTGGATTTTAAATAA

Protein Analysis

205

Amino Acids

22.78

Weight (kDa)

5.22

Isoelectric Point (pI)

26.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 2 - 126 7.1e-18 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016264)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g22960 FvH4_2g22990
rosa_chinensis RchiOBHm_Chr6g0289981
rosa_laevigata RLG00000012253 RLG00000012254 RLG00000012255
rosa_multiflora Rmu_sc0002902.1_g000066 Rmu_sc0003927.1_g000011
rosa_roxburghii Rroxscaffold_7G00177450
rosa_rugosa Rorug06G0208200
rosa_samantha Rh6AG319600 Rh6BG326700 Rh6CG332500 Rh6DG318400
rosa_wichuraiana Rw6G027580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 162
AccIII TCCGGA 1 cut(s) 388
AciI CCGC 5 cut(s) 160, 162, 311, 343, 538
AclWI GGATC 1 cut(s) 356
AcoI YGGCCR 1 cut(s) 157
AfaI GTAC 2 cut(s) 468, 544
AfiI CCNNNNNNNGG 4 cut(s) 82, 253, 287, 604
AflII CTTAAG 1 cut(s) 575
AgsI TTSAA 1 cut(s) 448
AhlI ACTAGT 1 cut(s) 68
AjnI CCWGG 1 cut(s) 508
AjuI GAANNNNNNNTTGG 2 cut(s) 548, 580
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 1 cut(s) 317
Alw44I GTGCAC 1 cut(s) 5
AlwI GGATC 1 cut(s) 356
Aor13HI TCCGGA 1 cut(s) 388
AoxI GGCC 2 cut(s) 93, 157
ApaLI GTGCAC 1 cut(s) 5
ApeKI GCWGC 2 cut(s) 173, 500
AspLEI GCGC 1 cut(s) 505
AspS9I GGNCC 1 cut(s) 48
AsuHPI GGTGA 2 cut(s) 427, 565
AvaII GGWCC 1 cut(s) 48
BaeGI GKGCMC 1 cut(s) 9
BanII GRGCYC 1 cut(s) 248
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 2 cut(s) 160, 512
BccI CCATC 3 cut(s) 190, 544, 609
BceAI ACGGC 3 cut(s) 80, 276, 525
BcgI CGANNNNNNTGC 2 cut(s) 54, 88
BciT130I CCWGG 1 cut(s) 510
BcoDI GTCTC 1 cut(s) 317
BcuI ACTAGT 1 cut(s) 68
BfaI CTAG 1 cut(s) 69
BfmI CTRYAG 1 cut(s) 588
BfrI CTTAAG 1 cut(s) 575
BglII AGATCT 1 cut(s) 336
BisI GCNGC 4 cut(s) 160, 163, 174, 501
BlsI GCNGC 4 cut(s) 161, 164, 175, 502
Bme1390I CCNGG 1 cut(s) 510
Bme18I GGWCC 1 cut(s) 48
BmgT120I GGNCC 1 cut(s) 48
BmiI GGNNCC 2 cut(s) 50, 381
BmrFI CCNGG 1 cut(s) 510
BsaI GGTCTC 1 cut(s) 317
BsaJI CCNNGG 3 cut(s) 160, 508, 509
BsaWI WCCGGW 1 cut(s) 388
BsaXI ACNNNNNCTCC 4 cut(s) 81, 111, 399, 429
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 253, 287, 604
Bse1I ACTGG 3 cut(s) 160, 172, 365
BseAI TCCGGA 1 cut(s) 388
BseBI CCWGG 1 cut(s) 510
BseDI CCNNGG 3 cut(s) 160, 508, 509
BseGI GGATG 1 cut(s) 201
BseLI CCNNNNNNNGG 4 cut(s) 82, 253, 287, 604
BseMII CTCAG 1 cut(s) 154
BseNI ACTGG 3 cut(s) 160, 172, 365
BseSI GKGCMC 1 cut(s) 9
BseXI GCAGC 2 cut(s) 160, 512
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 2 cut(s) 95, 159
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 1 cut(s) 389
BslFI GGGAC 3 cut(s) 264, 526, 578
BslI CCNNNNNNNGG 4 cut(s) 82, 253, 287, 604
BsmAI GTCTC 1 cut(s) 317
BsmFI GGGAC 3 cut(s) 264, 526, 578
BsmI GAATGC 1 cut(s) 313
BsnI GGCC 2 cut(s) 95, 159
Bso31I GGTCTC 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 9, 248
Bsp13I TCCGGA 1 cut(s) 388
Bsp143I GATC 4 cut(s) 79, 112, 336, 361
BspACI CCGC 5 cut(s) 160, 162, 311, 343, 538
BspANI GGCC 2 cut(s) 95, 159
BspCNI CTCAG 1 cut(s) 153
BspEI TCCGGA 1 cut(s) 388
BspFNI CGCG 1 cut(s) 162
BspLI GGNNCC 2 cut(s) 50, 381
BspPI GGATC 1 cut(s) 356
BspTI CTTAAG 1 cut(s) 575
BspTNI GGTCTC 1 cut(s) 317
BsrI ACTGG 3 cut(s) 160, 172, 365
BssECI CCNNGG 3 cut(s) 160, 508, 509
BssMI GATC 4 cut(s) 79, 112, 336, 361
Bst2UI CCWGG 1 cut(s) 510
Bst4CI ACNGT 7 cut(s) 11, 122, 430, 466, 520, 547, 572
BstAFI CTTAAG 1 cut(s) 575
BstDEI CTNAG 1 cut(s) 140
BstDSI CCRYGG 1 cut(s) 160
BstF5I GGATG 1 cut(s) 201
BstFNI CGCG 1 cut(s) 162
BstHHI GCGC 1 cut(s) 505
BstKTI GATC 4 cut(s) 82, 115, 339, 364
BstMAI GTCTC 1 cut(s) 317
BstMBI GATC 4 cut(s) 79, 112, 336, 361
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 1 cut(s) 510
BstSCI CCNGG 1 cut(s) 508
BstSFI CTRYAG 1 cut(s) 588
BstSLI GKGCMC 1 cut(s) 9
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 2 cut(s) 160, 512
BstX2I RGATCY 2 cut(s) 336, 361
BstYI RGATCY 2 cut(s) 336, 361
BsuRI GGCC 2 cut(s) 95, 159
BtgI CCRYGG 1 cut(s) 160
BtsCI GGATG 1 cut(s) 201
BtsIMutI CAGTG 3 cut(s) 165, 372, 552
CfoI GCGC 1 cut(s) 505
Cfr13I GGNCC 1 cut(s) 48
Cfr42I CCGCGG 1 cut(s) 163
Csp6I GTAC 2 cut(s) 467, 543
CviAII CATG 4 cut(s) 249, 328, 470, 491
CviJI RGCY 6 cut(s) 95, 159, 246, 276, 299, 382
CviKI_1 RGCY 6 cut(s) 95, 159, 246, 276, 299, 382
CviQI GTAC 2 cut(s) 467, 543
DdeI CTNAG 1 cut(s) 140
DpnI GATC 4 cut(s) 81, 114, 338, 363
DpnII GATC 4 cut(s) 79, 112, 336, 361
DraI TTTAAA 2 cut(s) 189, 613
EaeI YGGCCR 1 cut(s) 157
EciI GGCGGA 1 cut(s) 527
Eco24I GRGCYC 1 cut(s) 248
Eco31I GGTCTC 1 cut(s) 317
Eco32I GATATC 1 cut(s) 535
Eco47I GGWCC 1 cut(s) 48
EcoO109I RGGNCCY 1 cut(s) 48
EcoRII CCWGG 1 cut(s) 508
EcoRV GATATC 1 cut(s) 535
EcoT38I GRGCYC 1 cut(s) 248
FaeI CATG 4 cut(s) 252, 331, 473, 494
FaiI YATR 9 cut(s) 21, 152, 250, 329, 396, 402, 471, 492, 590
FaqI GGGAC 3 cut(s) 264, 526, 578
FatI CATG 4 cut(s) 248, 327, 469, 490
FauI CCCGC 1 cut(s) 336
Fnu4HI GCNGC 4 cut(s) 160, 163, 174, 501
FokI GGATG 1 cut(s) 208
FriOI GRGCYC 1 cut(s) 248
Fsp4HI GCNGC 4 cut(s) 160, 163, 174, 501
FspBI CTAG 1 cut(s) 69
GlaI GCGC 1 cut(s) 504
GluI GCNGC 4 cut(s) 160, 163, 174, 501
HaeIII GGCC 2 cut(s) 95, 159
HapII CCGG 1 cut(s) 389
HhaI GCGC 1 cut(s) 505
Hin1II CATG 4 cut(s) 252, 331, 473, 494
Hin6I GCGC 1 cut(s) 503
HinP1I GCGC 1 cut(s) 503
HincII GTYRAC 1 cut(s) 134
HindII GTYRAC 1 cut(s) 134
HinfI GANTC 1 cut(s) 451
HpaII CCGG 1 cut(s) 389
HphI GGTGA 2 cut(s) 427, 565
Hpy166II GTNNAC 4 cut(s) 7, 134, 484, 556
Hpy188I TCNGA 2 cut(s) 336, 379
Hpy188III TCNNGA 2 cut(s) 389, 605
Hpy8I GTNNAC 4 cut(s) 7, 134, 484, 556
HpyAV CCTTC 1 cut(s) 393
HpyCH4III ACNGT 7 cut(s) 11, 122, 430, 466, 520, 547, 572
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 6 cut(s) 7, 176, 182, 210, 490, 500
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 1 cut(s) 140
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 4 cut(s) 252, 331, 473, 494
HspAI GCGC 1 cut(s) 503
Kpn2I TCCGGA 1 cut(s) 388
KspI CCGCGG 1 cut(s) 163
Kzo9I GATC 4 cut(s) 79, 112, 336, 361
LmnI GCTCC 1 cut(s) 379
Lsp1109I GCAGC 2 cut(s) 160, 512
MaeI CTAG 1 cut(s) 69
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 4 cut(s) 122, 256, 346, 367
MalI GATC 4 cut(s) 81, 114, 338, 363
MboI GATC 4 cut(s) 79, 112, 336, 361
MboII GAAGA 3 cut(s) 460, 542, 593
MflI RGATCY 2 cut(s) 336, 361
MhlI GDGCHC 2 cut(s) 9, 248
MluCI AATT 5 cut(s) 183, 220, 373, 420, 433
MmeI TCCRAC 3 cut(s) 67, 268, 456
MnlI CCTC 4 cut(s) 85, 121, 406, 472
MroI TCCGGA 1 cut(s) 388
MseI TTAA 5 cut(s) 188, 303, 423, 576, 612
MspA1I CMGCKG 1 cut(s) 162
MspCI CTTAAG 1 cut(s) 575
MspI CCGG 1 cut(s) 389
MspR9I CCNGG 1 cut(s) 510
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 1 cut(s) 510
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 1 cut(s) 179
NdeII GATC 4 cut(s) 79, 112, 336, 361
NlaIII CATG 4 cut(s) 252, 331, 473, 494
NlaIV GGNNCC 2 cut(s) 50, 381
NmuCI GTSAC 3 cut(s) 256, 346, 367
PasI CCCWGGG 1 cut(s) 509
PctI GAATGC 1 cut(s) 313
PfeI GAWTC 1 cut(s) 451
PkrI GCNGC 4 cut(s) 161, 164, 175, 502
PpuMI RGGWCCY 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 48
Psp6I CCWGG 1 cut(s) 508
PspGI CCWGG 1 cut(s) 508
PspN4I GGNNCC 2 cut(s) 50, 381
PspPI GGNCC 1 cut(s) 48
PspPPI RGGWCCY 1 cut(s) 48
PsuI RGATCY 2 cut(s) 336, 361
RsaI GTAC 2 cut(s) 468, 544
RsaNI GTAC 2 cut(s) 467, 543
SacII CCGCGG 1 cut(s) 163
SaqAI TTAA 5 cut(s) 188, 303, 423, 576, 612
SatI GCNGC 4 cut(s) 160, 163, 174, 501
Sau3AI GATC 4 cut(s) 79, 112, 336, 361
Sau96I GGNCC 1 cut(s) 48
ScrFI CCNGG 1 cut(s) 510
SduI GDGCHC 2 cut(s) 9, 248
SetI ASST 4 cut(s) 45, 87, 417, 483
SfcI CTRYAG 1 cut(s) 588
Sfr303I CCGCGG 1 cut(s) 163
SgrBI CCGCGG 1 cut(s) 163
SinI GGWCC 1 cut(s) 48
SmlI CTYRAG 1 cut(s) 575
SmoI CTYRAG 1 cut(s) 575
SpeI ACTAGT 1 cut(s) 68
Sse9I AATT 5 cut(s) 183, 220, 373, 420, 433
SsiI CCGC 5 cut(s) 160, 162, 311, 343, 538
SspMI CTAG 1 cut(s) 69
StyD4I CCNGG 1 cut(s) 508
TaaI ACNGT 7 cut(s) 11, 122, 430, 466, 520, 547, 572
TaiI ACGT 1 cut(s) 45
TasI AATT 5 cut(s) 183, 220, 373, 420, 433
TauI GCSGC 2 cut(s) 162, 165
TfiI GAWTC 1 cut(s) 451
Tru1I TTAA 5 cut(s) 188, 303, 423, 576, 612
Tru9I TTAA 5 cut(s) 188, 303, 423, 576, 612
TscAI CASTG 3 cut(s) 172, 372, 552
TseFI GTSAC 3 cut(s) 256, 346, 367
TseI GCWGC 2 cut(s) 173, 500
Tsp45I GTSAC 3 cut(s) 256, 346, 367
TspDTI ATGAA 2 cut(s) 369, 411
TspRI CASTG 3 cut(s) 172, 372, 552
Vha464I CTTAAG 1 cut(s) 575
VneI GTGCAC 1 cut(s) 5
VpaK11BI GGWCC 1 cut(s) 48
XcmI CCANNNNNNNNNTGG 1 cut(s) 83
XspI CTAG 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.