RLG00000016565

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018345
Physical Location & Seq
Forward (+)
7697766 .. 7702316
4551 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016565

Sequence Viewer

Length: 1365 bp
ATGGAAATTCTCAGATCACAACTCTATAAAAGCCTTTTTGAAGTTCAGCTCTCAGTGGCTTATGGGATCTGTGTACTACATCTGATTTCTATATTCAACTGGATGCAAAGCAAGCTTACTGGGAAACCCCAGATTAAGAAACCAAACTTGAAACTGTTTGATGTGCAAAAACCTCCCAAAGAAGATCAACCAATTAGTGAATGGCCTCATGGACTGTTGACAATTGGAACATTAGGAAGTAATCATGAATCAAAAGAAGATCTACATGCATGGGCTAATCCACCACCCTCCCCTAGAGATCCTCTACATGATTTTACACCAGCGGAAGCAACACACATTCAGAATGAGTTGAACTCATTCTTCAATGAACATGTTGATCAATCAAGTTCTTCAGCTGCAGAACTGGGCAAGTTTCTTAACAGGCAATCAAGCTTGAAGTTGACTGAAAGAACTAATAGCAATGCTGCTGATTCTGATGAGCCTAAGGAGCATTTTAATAGTCGATTCCAGTGTAGTGCAAGTGTGGTTCTTAGTAGAGGGAAAGATGTCAATTGCGTGGAGAACACAAACACTGCCATGGGAAAGAAGTCATTGTCTTTTCTTCTGAAAAAAATGTTTATTTGCAGTAGTGGGTTTGCACCACCAGTTGCTGCTCCTGCTCTTAGAGATCCAATCCCTGAATCAAGAATGGAGAAGTTATTGAGGGCTATACTTCACAAGAAGATCTATCCCCAGCACAGTTCAAATTCAACAGTTTCGAAGAAAAAGTACTTGGAGAACAGACACATCACCAAGCCTGTCACTGCCAGTGAGGAGCAACTAGATCCTAAACGAGACGATTCAGACTCGGATGACGATGGAAGTAAATGGGACAGGACTGATTCTGAATGGTTGGTGGGTTTGATCTACCGGAGCACTGTGTCAGTGAAAGTCTTGAGCTTTGAGACTTTTAGAGGTGCTTCTCTGCTTAAGCTTAATGCCATTGAAGAAGGCGCAGAAGACTCTCTCCTCTCCACTTCTCTGGCGAGCTATCTTGACAAAAAGCTTCTTGTCTTATTGCGAGATGGGCGAAAGCTGTTGGGGTTACTGCGGTCTTTTGACCAGTTTGCTAATGTTGTGCTTGAAGGTGCATGTGAACGAGTTATTGTCGGTGATCTTTATTGTGACATCCCATTAGGTCTGTATGTAATCCGTGGGGAGAATGTAGTCTTAATTGGAGAACTGGAATTGAATAAAGAGGAGCTTCCCCCGCATATGACTCTGGTACCCGAAGCAGAGATAAAAAGGGCTCAGAGAGCAGAGAGGGATGCTTCAGACTTAAAAGGCTCCATGAGAAAAAGAATGGAGTTCCTTGATTTCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

455

Amino Acids

51.05

Weight (kDa)

6.22

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM PF01423 340 - 406 1.6e-18 LSM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09000
prunus_persica Prupe.7G195900_v2.0.a1
rosa_laevigata RLG00000016565
rosa_multiflora Rmu_co8252679.1_g000001
rosa_rugosa Rorug02G0050800
rosa_samantha Rh2AG099000 Rh2BG100200 Rh2CG102300 Rh2DG099800
rosa_wichuraiana Rw2G007480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1264
AccB1I GGYRCC 1 cut(s) 1264
AciI CCGC 3 cut(s) 323, 1090, 1250
AclWI GGATC 4 cut(s) 74, 293, 662, 818
AcsI RAATTY 2 cut(s) 6, 745
AcuI CTGAAG 2 cut(s) 375, 1296
AfaI GTAC 3 cut(s) 75, 770, 1266
AflII CTTAAG 1 cut(s) 968
AflIII ACRYGT 1 cut(s) 370
AjuI GAANNNNNNNTTGG 2 cut(s) 755, 787
Alw21I GWGCWC 1 cut(s) 917
Alw26I GTCTC 2 cut(s) 828, 938
AlwI GGATC 4 cut(s) 74, 293, 662, 818
AlwNI CAGNNNCTG 1 cut(s) 650
AoxI GGCC 1 cut(s) 203
ApeKI GCWGC 3 cut(s) 395, 464, 650
ApoI RAATTY 2 cut(s) 6, 745
Asp700I GAANNNNTTC 1 cut(s) 356
Asp718I GGTACC 1 cut(s) 1264
AspLEI GCGC 1 cut(s) 995
AsuHPI GGTGA 2 cut(s) 781, 1163
AsuII TTCGAA 1 cut(s) 758
AxyI CCTNAGG 1 cut(s) 483
BaeI ACNNNNGTAYC 2 cut(s) 1248, 1281
BanI GGYRCC 1 cut(s) 1264
BanII GRGCYC 1 cut(s) 1291
BarI GAAGNNNNNNTAC 2 cut(s) 752, 784
BbsI GAAGAC 1 cut(s) 1005
Bbv12I GWGCWC 1 cut(s) 917
BbvI GCAGC 3 cut(s) 382, 451, 637
BccI CCATC 2 cut(s) 851, 1058
BclI TGATCA 1 cut(s) 376
BcoDI GTCTC 2 cut(s) 828, 938
BfaI CTAG 2 cut(s) 294, 821
BfmI CTRYAG 1 cut(s) 396
BfrI CTTAAG 1 cut(s) 968
BglII AGATCT 2 cut(s) 259, 723
BisI GCNGC 3 cut(s) 396, 465, 651
BlsI GCNGC 3 cut(s) 397, 466, 652
BmcAI AGTACT 1 cut(s) 770
BmiI GGNNCC 2 cut(s) 1266, 1327
BmrI ACTGGG 2 cut(s) 129, 413
BmsI GCATC 2 cut(s) 93, 1297
BmuI ACTGGG 2 cut(s) 129, 413
BpiI GAAGAC 1 cut(s) 1005
BplI GAGNNNNNCTC 2 cut(s) 338, 370
Bpu14I TTCGAA 1 cut(s) 758
BpuEI CTTGAG 1 cut(s) 955
BsaJI CCNNGG 2 cut(s) 576, 1192
BsaWI WCCGGW 1 cut(s) 909
Bse1I ACTGG 8 cut(s) 104, 124, 408, 508, 644, 807, 1102, 1227
Bse21I CCTNAGG 1 cut(s) 483
Bse3DI GCAATG 1 cut(s) 466
BseDI CCNNGG 2 cut(s) 576, 1192
BseGI GGATG 4 cut(s) 108, 856, 1167, 1312
BseMI GCAATG 1 cut(s) 466
BseMII CTCAG 3 cut(s) 25, 66, 1304
BseNI ACTGG 8 cut(s) 104, 124, 408, 508, 644, 807, 1102, 1227
BseRI GAGGAG 3 cut(s) 827, 998, 1253
BseXI GCAGC 3 cut(s) 382, 451, 637
BseYI CCCAGC 1 cut(s) 732
BshFI GGCC 1 cut(s) 205
BshNI GGYRCC 1 cut(s) 1264
BsiHKAI GWGCWC 1 cut(s) 917
BsiSI CCGG 1 cut(s) 910
BslFI GGGAC 1 cut(s) 884
BsmAI GTCTC 2 cut(s) 828, 938
BsmBI CGTCTC 1 cut(s) 828
BsmFI GGGAC 1 cut(s) 884
BsnI GGCC 1 cut(s) 205
Bsp119I TTCGAA 1 cut(s) 758
Bsp1286I GDGCHC 2 cut(s) 917, 1291
Bsp19I CCATGG 1 cut(s) 576
BspACI CCGC 3 cut(s) 323, 1090, 1250
BspANI GGCC 1 cut(s) 205
BspCNI CTCAG 3 cut(s) 24, 65, 1303
BspHI TCATGA 1 cut(s) 244
BspLI GGNNCC 2 cut(s) 1266, 1327
BspMAI CTGCAG 1 cut(s) 400
BspPI GGATC 4 cut(s) 74, 293, 662, 818
BspT104I TTCGAA 1 cut(s) 758
BspT107I GGYRCC 1 cut(s) 1264
BspTI CTTAAG 1 cut(s) 968
BsrDI GCAATG 1 cut(s) 466
BsrI ACTGG 8 cut(s) 104, 124, 408, 508, 644, 807, 1102, 1227
BssECI CCNNGG 2 cut(s) 576, 1192
BssT1I CCWWGG 1 cut(s) 576
Bst4CI ACNGT 5 cut(s) 156, 216, 740, 754, 919
BstAFI CTTAAG 1 cut(s) 968
BstBI TTCGAA 1 cut(s) 758
BstC8I GCNNGC 2 cut(s) 113, 1027
BstDEI CTNAG 6 cut(s) 11, 52, 483, 530, 662, 1290
BstDSI CCRYGG 2 cut(s) 576, 1192
BstF5I GGATG 4 cut(s) 108, 856, 1167, 1312
BstHHI GCGC 1 cut(s) 995
BstMAI GTCTC 2 cut(s) 828, 938
BstMWI GCNNNNNNNGC 6 cut(s) 112, 487, 656, 1066, 1249, 1295
BstNSI RCATGY 3 cut(s) 269, 374, 1134
BstSFI CTRYAG 1 cut(s) 396
BstV1I GCAGC 3 cut(s) 382, 451, 637
BstV2I GAAGAC 1 cut(s) 1005
BstX2I RGATCY 6 cut(s) 66, 259, 298, 667, 723, 823
BstXI CCANNNNNNTGG 1 cut(s) 1021
BstYI RGATCY 6 cut(s) 66, 259, 298, 667, 723, 823
Bsu36I CCTNAGG 1 cut(s) 483
BsuRI GGCC 1 cut(s) 205
BtgI CCRYGG 2 cut(s) 576, 1192
BtsCI GGATG 4 cut(s) 108, 856, 1167, 1312
BtsI GCAGTG 2 cut(s) 570, 801
BtsIMutI CAGTG 7 cut(s) 60, 515, 570, 801, 814, 915, 930
Cac8I GCNNGC 2 cut(s) 113, 1027
CaiI CAGNNNCTG 1 cut(s) 650
CciI TCATGA 1 cut(s) 244
CfoI GCGC 1 cut(s) 995
Csp6I GTAC 3 cut(s) 74, 769, 1265
CviAII CATG 9 cut(s) 209, 245, 266, 270, 308, 371, 577, 1131, 1330
CviQI GTAC 3 cut(s) 74, 769, 1265
DdeI CTNAG 6 cut(s) 11, 52, 483, 530, 662, 1290
Eco130I CCWWGG 1 cut(s) 576
Eco24I GRGCYC 1 cut(s) 1291
Eco57I CTGAAG 2 cut(s) 375, 1296
Eco81I CCTNAGG 1 cut(s) 483
EcoT14I CCWWGG 1 cut(s) 576
EcoT22I ATGCAT 1 cut(s) 271
EcoT38I GRGCYC 1 cut(s) 1291
ErhI CCWWGG 1 cut(s) 576
Esp3I CGTCTC 1 cut(s) 828
FaeI CATG 9 cut(s) 212, 248, 269, 273, 311, 374, 580, 1134, 1333
FalI AAGNNNNNCTT 2 cut(s) 1227, 1259
FaqI GGGAC 1 cut(s) 884
FatI CATG 9 cut(s) 208, 244, 265, 269, 307, 370, 576, 1130, 1329
FauI CCCGC 1 cut(s) 1257
FauNDI CATATG 1 cut(s) 1254
FbaI TGATCA 1 cut(s) 376
Fnu4HI GCNGC 3 cut(s) 396, 465, 651
FokI GGATG 4 cut(s) 115, 863, 1154, 1319
FriOI GRGCYC 1 cut(s) 1291
Fsp4HI GCNGC 3 cut(s) 396, 465, 651
FspBI CTAG 2 cut(s) 294, 821
GlaI GCGC 1 cut(s) 994
GluI GCNGC 3 cut(s) 396, 465, 651
GsaI CCCAGC 1 cut(s) 736
HaeIII GGCC 1 cut(s) 205
HapII CCGG 1 cut(s) 910
HhaI GCGC 1 cut(s) 995
Hin1II CATG 9 cut(s) 212, 248, 269, 273, 311, 374, 580, 1134, 1333
Hin6I GCGC 1 cut(s) 993
HinP1I GCGC 1 cut(s) 993
HincII GTYRAC 2 cut(s) 219, 441
HindII GTYRAC 2 cut(s) 219, 441
HindIII AAGCTT 4 cut(s) 113, 430, 971, 1043
HinfI GANTC 9 cut(s) 248, 470, 504, 680, 839, 845, 881, 1001, 1258
HpaII CCGG 1 cut(s) 910
HphI GGTGA 2 cut(s) 781, 1163
Hpy166II GTNNAC 4 cut(s) 74, 219, 441, 1136
Hpy188III TCNNGA 4 cut(s) 245, 684, 934, 1034
Hpy8I GTNNAC 4 cut(s) 74, 219, 441, 1136
HpyAV CCTTC 2 cut(s) 983, 1118
HpyCH4III ACNGT 5 cut(s) 156, 216, 740, 754, 919
HpyCH4V TGCA 8 cut(s) 106, 166, 269, 398, 518, 624, 638, 1130
HpyF10VI GCNNNNNNNGC 6 cut(s) 112, 487, 656, 1066, 1249, 1295
HpyF3I CTNAG 6 cut(s) 11, 52, 483, 530, 662, 1290
Hsp92II CATG 9 cut(s) 212, 248, 269, 273, 311, 374, 580, 1134, 1333
HspAI GCGC 1 cut(s) 993
KpnI GGTACC 1 cut(s) 1268
Ksp22I TGATCA 1 cut(s) 376
LmnI GCTCC 6 cut(s) 487, 658, 814, 912, 1240, 1331
Lsp1109I GCAGC 3 cut(s) 382, 451, 637
LweI GCATC 2 cut(s) 93, 1297
MaeI CTAG 2 cut(s) 294, 821
MaeIII GTNAC 3 cut(s) 799, 1083, 1163
MboII GAAGA 9 cut(s) 194, 269, 352, 381, 593, 733, 772, 998, 1010
MfeI CAATTG 2 cut(s) 222, 550
MflI RGATCY 6 cut(s) 66, 259, 298, 667, 723, 823
MhlI GDGCHC 2 cut(s) 917, 1291
MluCI AATT 7 cut(s) 6, 192, 222, 550, 745, 1212, 1226
MlyI GAGTC 3 cut(s) 839, 995, 1252
Mph1103I ATGCAT 1 cut(s) 271
MroXI GAANNNNTTC 1 cut(s) 356
MseI TTAA 8 cut(s) 135, 417, 495, 969, 975, 1211, 1319, 1363
MslI CAYNNNNRTG 1 cut(s) 575
MspA1I CMGCKG 2 cut(s) 323, 395
MspCI CTTAAG 1 cut(s) 968
MspI CCGG 1 cut(s) 910
MunI CAATTG 2 cut(s) 222, 550
MwoI GCNNNNNNNGC 6 cut(s) 112, 487, 656, 1066, 1249, 1295
NcoI CCATGG 1 cut(s) 576
NdeI CATATG 1 cut(s) 1254
NlaIII CATG 9 cut(s) 212, 248, 269, 273, 311, 374, 580, 1134, 1333
NlaIV GGNNCC 2 cut(s) 1266, 1327
NmuCI GTSAC 2 cut(s) 799, 1163
NsiI ATGCAT 1 cut(s) 271
NspI RCATGY 3 cut(s) 269, 374, 1134
NspV TTCGAA 1 cut(s) 758
PagI TCATGA 1 cut(s) 244
PciI ACATGT 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 356
PfeI GAWTC 6 cut(s) 248, 470, 504, 680, 839, 881
PkrI GCNGC 3 cut(s) 397, 466, 652
PleI GAGTC 3 cut(s) 839, 995, 1252
PpsI GAGTC 3 cut(s) 839, 995, 1252
PscI ACATGT 1 cut(s) 370
PspFI CCCAGC 1 cut(s) 732
PspN4I GGNNCC 2 cut(s) 1266, 1327
PstI CTGCAG 1 cut(s) 400
PstNI CAGNNNCTG 1 cut(s) 650
PsuI RGATCY 6 cut(s) 66, 259, 298, 667, 723, 823
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 3 cut(s) 75, 770, 1266
RsaNI GTAC 3 cut(s) 74, 769, 1265
RseI CAYNNNNRTG 1 cut(s) 575
SaqAI TTAA 8 cut(s) 135, 417, 495, 969, 975, 1211, 1319, 1363
SatI GCNGC 3 cut(s) 396, 465, 651
ScaI AGTACT 1 cut(s) 770
SchI GAGTC 3 cut(s) 839, 995, 1252
SduI GDGCHC 2 cut(s) 917, 1291
SfaNI GCATC 2 cut(s) 93, 1297
SfcI CTRYAG 1 cut(s) 396
SfuI TTCGAA 1 cut(s) 758
SmiMI CAYNNNNRTG 1 cut(s) 575
SmlI CTYRAG 2 cut(s) 934, 968
SmoI CTYRAG 2 cut(s) 934, 968
Sse9I AATT 7 cut(s) 6, 192, 222, 550, 745, 1212, 1226
SsiI CCGC 3 cut(s) 323, 1090, 1250
SspMI CTAG 2 cut(s) 294, 821
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 5 cut(s) 156, 216, 740, 754, 919
TaqI TCGA 3 cut(s) 502, 758, 1359
TasI AATT 7 cut(s) 6, 192, 222, 550, 745, 1212, 1226
TatI WGTACW 2 cut(s) 73, 768
TfiI GAWTC 6 cut(s) 248, 470, 504, 680, 839, 881
Tru1I TTAA 8 cut(s) 135, 417, 495, 969, 975, 1211, 1319, 1363
Tru9I TTAA 8 cut(s) 135, 417, 495, 969, 975, 1211, 1319, 1363
TscAI CASTG 7 cut(s) 60, 515, 577, 808, 814, 922, 930
TseFI GTSAC 2 cut(s) 799, 1163
TseI GCWGC 3 cut(s) 395, 464, 650
Tsp45I GTSAC 2 cut(s) 799, 1163
TspDTI ATGAA 2 cut(s) 261, 381
TspGWI ACGGA 1 cut(s) 1181
TspRI CASTG 7 cut(s) 60, 515, 577, 808, 814, 922, 930
Vha464I CTTAAG 1 cut(s) 968
XapI RAATTY 2 cut(s) 6, 745
XceI RCATGY 3 cut(s) 269, 374, 1134
XcmI CCANNNNNNNNNTGG 1 cut(s) 198
XmnI GAANNNNTTC 1 cut(s) 356
XspI CTAG 2 cut(s) 294, 821
ZrmI AGTACT 1 cut(s) 770
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.