Rh2AG099000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0018345
Physical Location & Seq
Forward (+)
8379886 .. 8384621
4736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG099000.1

Sequence Viewer

Length: 1218 bp
ATGAAGATATTCAGCTGGATGCAAAGCAAGCTTACTGGGAAACCCCAGATTAAGAAACCAAACTTGAAACTGTTTGATGATGATCACAAAGTGCAAAAACCTCCCAAAGAAGATCAACCAATTAGTGAATGGCCTCATGGACTGTTGACAATTGGAACATTAGGAAGTAATCATGAATCAAAAGAAGATCTACATGCATGGGCTAATCCACCACCCTCCCCTAGAGATCCTCTACATGATTTTACACCAGCGGAAGCAACACACATTCAGAATGAGTTGAACTCATTCTTCAATGAACATGTTGATCAATCAAGTTCTTCAGCTGCAGAACTGGGCAAGTTTCTTAACAGGCAATCAAGCTTGAAGTTAACTGAAAGAACTAATAATAGCAATGCTGCTGATTCTGATGAGCCTAAGGAGCATTTTAATAGTCGATTCCAGCGCAGCGCAAGTGTGGTTCTTAGTCGAGGGAAAGATGTCAATTGCTTGGAGAACACAAACACTGCCATAGGAAAGAAGTCATTGACTTTTCTTCTGAAAAAAATGTTTGTTTGCAGTAGTGGGTTTGCAGCACCAGTTGCTGCTCCTGCTCTAAGAGATCCAATCCCTGAATCAAGAATGGAGAAGTTATTGAGGGCTATACTTCACAAGAAGATCTGTCCCCAGCAAAGTTCAAATTCAACAGTTTCCAAGAAAAAGTACTTGGAGAACAGACACATCACCAAGTCTGTCACTGCCACTGAGGAGCAACTAGATCCTAAACGAGACGATTCAGACTCGGATGACGATGGAAGTAAATGGGACAAGACTGATTCTGAATTTTACTACTTGATGTCGTGGGCAGGCCCAGAAGACTCTCTCCTCTCCACTTCTCTGGCGAGCTATCTTGACAAAAAGCTTCTTGTCTTATTGCGAGATGGGCGAAAGCTCTTGGGGTTACTACGGTCTTTTGACCAGTTTGCTAATGTTGTGCTTGAAGGTGCATGTGAACGAGTTATTGTCGGTGATCTTTATTGTGACATCCCATTAGGTCTGTATGTAATCCGTGGGGAGAATGTAGTCTTAATTGGAGAACTGGAATTGAATAAAGAGGAGCTTCCCCCGCATATGACTCTGGTACCCGAAGCAGAGATAAAAAGGGCTCAGAAAGCAGAGAGGGATGCTTCCGACTTAAAAGGCTCCATGAGAAAAAGAATGGAGTTCCTTGATTTCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

405

Amino Acids

45.62

Weight (kDa)

6.17

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM PF01423 291 - 357 1.4e-18 LSM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09000
prunus_persica Prupe.7G195900_v2.0.a1
rosa_laevigata RLG00000016565
rosa_multiflora Rmu_co8252679.1_g000001
rosa_rugosa Rorug02G0050800
rosa_samantha Rh2AG099000 Rh2BG100200 Rh2CG102300 Rh2DG099800
rosa_wichuraiana Rw2G007480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1117
AccB1I GGYRCC 1 cut(s) 1117
AciI CCGC 2 cut(s) 251, 1103
AclWI GGATC 3 cut(s) 221, 593, 749
AcsI RAATTY 2 cut(s) 676, 818
AcuI CTGAAG 1 cut(s) 303
AdeI CACNNNGTG 1 cut(s) 91
AfaI GTAC 2 cut(s) 701, 1119
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 8 cut(s) 67, 280, 292, 364, 675, 681, 977, 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
AluBI AGCT 8 cut(s) 15, 31, 323, 360, 882, 898, 928, 1096
AluI AGCT 8 cut(s) 15, 31, 323, 360, 882, 898, 928, 1096
Alw26I GTCTC 1 cut(s) 759
AlwI GGATC 3 cut(s) 221, 593, 749
AlwNI CAGNNNCTG 1 cut(s) 581
AoxI GGCC 2 cut(s) 131, 844
ApeKI GCWGC 5 cut(s) 323, 395, 444, 569, 581
ApoI RAATTY 2 cut(s) 676, 818
Asp700I GAANNNNTTC 2 cut(s) 8, 284
Asp718I GGTACC 1 cut(s) 1117
AspLEI GCGC 2 cut(s) 444, 449
AspS9I GGNCC 1 cut(s) 845
AsuHPI GGTGA 2 cut(s) 712, 1016
AxyI CCTNAGG 1 cut(s) 414
BaeI ACNNNNGTAYC 2 cut(s) 1101, 1134
BanI GGYRCC 1 cut(s) 1117
BanII GRGCYC 1 cut(s) 1144
BbsI GAAGAC 1 cut(s) 858
BbvI GCAGC 5 cut(s) 310, 382, 456, 568, 581
BccI CCATC 2 cut(s) 782, 911
BclI TGATCA 2 cut(s) 82, 304
BcoDI GTCTC 1 cut(s) 759
BfaI CTAG 2 cut(s) 222, 752
BfmI CTRYAG 1 cut(s) 324
BglII AGATCT 2 cut(s) 187, 654
BisI GCNGC 5 cut(s) 324, 396, 445, 570, 582
BlsI GCNGC 5 cut(s) 325, 397, 446, 571, 583
BmcAI AGTACT 1 cut(s) 701
BmgT120I GGNCC 1 cut(s) 845
BmiI GGNNCC 2 cut(s) 1119, 1180
BmrI ACTGGG 2 cut(s) 45, 341
BmsI GCATC 2 cut(s) 9, 1150
BmuI ACTGGG 2 cut(s) 45, 341
BpiI GAAGAC 1 cut(s) 858
BplI GAGNNNNNCTC 2 cut(s) 266, 298
BsaBI GATNNNNATC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 1045
Bse1I ACTGG 5 cut(s) 40, 336, 575, 955, 1080
Bse21I CCTNAGG 1 cut(s) 414
Bse3DI GCAATG 1 cut(s) 397
Bse8I GATNNNNATC 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 1045
BseGI GGATG 4 cut(s) 24, 787, 1020, 1165
BseJI GATNNNNATC 1 cut(s) 81
BseMI GCAATG 1 cut(s) 397
BseMII CTCAG 2 cut(s) 732, 1157
BseNI ACTGG 5 cut(s) 40, 336, 575, 955, 1080
BseRI GAGGAG 3 cut(s) 758, 851, 1106
BseXI GCAGC 5 cut(s) 310, 382, 456, 568, 581
BseYI CCCAGC 1 cut(s) 663
BshFI GGCC 2 cut(s) 133, 846
BshNI GGYRCC 1 cut(s) 1117
BslFI GGGAC 2 cut(s) 645, 815
BsmAI GTCTC 1 cut(s) 759
BsmBI CGTCTC 1 cut(s) 759
BsmFI GGGAC 2 cut(s) 645, 815
BsnI GGCC 2 cut(s) 133, 846
Bsp1286I GDGCHC 1 cut(s) 1144
Bsp143I GATC 9 cut(s) 82, 112, 187, 226, 304, 598, 654, 754, 1006
BspACI CCGC 2 cut(s) 251, 1103
BspANI GGCC 2 cut(s) 133, 846
BspCNI CTCAG 2 cut(s) 733, 1156
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 2 cut(s) 1119, 1180
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 3 cut(s) 221, 593, 749
BspT107I GGYRCC 1 cut(s) 1117
BsrDI GCAATG 1 cut(s) 397
BsrI ACTGG 5 cut(s) 40, 336, 575, 955, 1080
BssECI CCNNGG 1 cut(s) 1045
BssMI GATC 9 cut(s) 82, 112, 187, 226, 304, 598, 654, 754, 1006
Bst4CI ACNGT 4 cut(s) 72, 144, 685, 945
BstAPI GCANNNNNTGC 1 cut(s) 578
BstC8I GCNNGC 3 cut(s) 29, 844, 880
BstDEI CTNAG 5 cut(s) 414, 461, 593, 741, 1143
BstDSI CCRYGG 1 cut(s) 1045
BstF5I GGATG 4 cut(s) 24, 787, 1020, 1165
BstHHI GCGC 2 cut(s) 444, 449
BstKTI GATC 9 cut(s) 85, 115, 190, 229, 307, 601, 657, 757, 1009
BstMAI GTCTC 1 cut(s) 759
BstMBI GATC 9 cut(s) 82, 112, 187, 226, 304, 598, 654, 754, 1006
BstMWI GCNNNNNNNGC 7 cut(s) 28, 418, 578, 587, 919, 1102, 1148
BstNSI RCATGY 3 cut(s) 197, 302, 987
BstSFI CTRYAG 1 cut(s) 324
BstV1I GCAGC 5 cut(s) 310, 382, 456, 568, 581
BstV2I GAAGAC 1 cut(s) 858
BstX2I RGATCY 5 cut(s) 187, 226, 598, 654, 754
BstXI CCANNNNNNTGG 1 cut(s) 874
BstYI RGATCY 5 cut(s) 187, 226, 598, 654, 754
Bsu36I CCTNAGG 1 cut(s) 414
BsuRI GGCC 2 cut(s) 133, 846
BtgI CCRYGG 1 cut(s) 1045
BtsCI GGATG 4 cut(s) 24, 787, 1020, 1165
BtsI GCAGTG 2 cut(s) 501, 732
BtsIMutI CAGTG 3 cut(s) 501, 732, 738
Cac8I GCNNGC 3 cut(s) 29, 844, 880
CaiI CAGNNNCTG 1 cut(s) 581
CciI TCATGA 1 cut(s) 172
CfoI GCGC 2 cut(s) 444, 449
Cfr13I GGNCC 1 cut(s) 845
Csp6I GTAC 2 cut(s) 700, 1118
CviAII CATG 8 cut(s) 137, 173, 194, 198, 236, 299, 984, 1183
CviQI GTAC 2 cut(s) 700, 1118
DdeI CTNAG 5 cut(s) 414, 461, 593, 741, 1143
DpnI GATC 9 cut(s) 84, 114, 189, 228, 306, 600, 656, 756, 1008
DpnII GATC 9 cut(s) 82, 112, 187, 226, 304, 598, 654, 754, 1006
DraIII CACNNNGTG 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 1144
Eco57I CTGAAG 1 cut(s) 303
Eco81I CCTNAGG 1 cut(s) 414
EcoT22I ATGCAT 1 cut(s) 199
EcoT38I GRGCYC 1 cut(s) 1144
Esp3I CGTCTC 1 cut(s) 759
FaeI CATG 8 cut(s) 140, 176, 197, 201, 239, 302, 987, 1186
FalI AAGNNNNNCTT 2 cut(s) 1080, 1112
FaqI GGGAC 2 cut(s) 645, 815
FatI CATG 8 cut(s) 136, 172, 193, 197, 235, 298, 983, 1182
FauI CCCGC 1 cut(s) 1110
FauNDI CATATG 1 cut(s) 1107
FbaI TGATCA 2 cut(s) 82, 304
Fnu4HI GCNGC 5 cut(s) 324, 396, 445, 570, 582
FokI GGATG 4 cut(s) 31, 794, 1007, 1172
FriOI GRGCYC 1 cut(s) 1144
Fsp4HI GCNGC 5 cut(s) 324, 396, 445, 570, 582
FspBI CTAG 2 cut(s) 222, 752
GlaI GCGC 2 cut(s) 443, 448
GluI GCNGC 5 cut(s) 324, 396, 445, 570, 582
GsaI CCCAGC 1 cut(s) 667
HaeIII GGCC 2 cut(s) 133, 846
HhaI GCGC 2 cut(s) 444, 449
Hin1II CATG 8 cut(s) 140, 176, 197, 201, 239, 302, 987, 1186
Hin6I GCGC 2 cut(s) 442, 447
HinP1I GCGC 2 cut(s) 442, 447
HincII GTYRAC 2 cut(s) 147, 369
HindII GTYRAC 2 cut(s) 147, 369
HindIII AAGCTT 3 cut(s) 29, 358, 896
HinfI GANTC 9 cut(s) 176, 401, 435, 611, 770, 776, 812, 854, 1111
HpaI GTTAAC 1 cut(s) 369
HphI GGTGA 2 cut(s) 712, 1016
Hpy166II GTNNAC 3 cut(s) 147, 369, 989
Hpy188I TCNGA 8 cut(s) 270, 406, 537, 775, 781, 817, 1146, 1168
Hpy188III TCNNGA 3 cut(s) 173, 615, 887
Hpy8I GTNNAC 3 cut(s) 147, 369, 989
HpyAV CCTTC 1 cut(s) 971
HpyCH4III ACNGT 4 cut(s) 72, 144, 685, 945
HpyCH4V TGCA 7 cut(s) 22, 94, 197, 326, 555, 569, 983
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 418, 578, 587, 919, 1102, 1148
HpyF3I CTNAG 5 cut(s) 414, 461, 593, 741, 1143
Hsp92II CATG 8 cut(s) 140, 176, 197, 201, 239, 302, 987, 1186
HspAI GCGC 2 cut(s) 442, 447
KpnI GGTACC 1 cut(s) 1121
Ksp22I TGATCA 2 cut(s) 82, 304
KspAI GTTAAC 1 cut(s) 369
Kzo9I GATC 9 cut(s) 82, 112, 187, 226, 304, 598, 654, 754, 1006
LmnI GCTCC 5 cut(s) 418, 589, 745, 1093, 1184
Lsp1109I GCAGC 5 cut(s) 310, 382, 456, 568, 581
LweI GCATC 2 cut(s) 9, 1150
MaeI CTAG 2 cut(s) 222, 752
MaeIII GTNAC 3 cut(s) 730, 936, 1016
MalI GATC 9 cut(s) 84, 114, 189, 228, 306, 600, 656, 756, 1008
MboI GATC 9 cut(s) 82, 112, 187, 226, 304, 598, 654, 754, 1006
MboII GAAGA 8 cut(s) 16, 122, 197, 280, 309, 524, 664, 863
MfeI CAATTG 2 cut(s) 150, 481
MflI RGATCY 5 cut(s) 187, 226, 598, 654, 754
MhlI GDGCHC 1 cut(s) 1144
MluCI AATT 7 cut(s) 120, 150, 481, 676, 818, 1065, 1079
MlyI GAGTC 3 cut(s) 770, 848, 1105
MmeI TCCRAC 1 cut(s) 1191
Mph1103I ATGCAT 1 cut(s) 199
MroXI GAANNNNTTC 2 cut(s) 8, 284
MseI TTAA 7 cut(s) 51, 345, 368, 426, 1064, 1172, 1216
MspA1I CMGCKG 3 cut(s) 15, 251, 323
MteI GCGCNGCGC 1 cut(s) 445
MunI CAATTG 2 cut(s) 150, 481
MwoI GCNNNNNNNGC 7 cut(s) 28, 418, 578, 587, 919, 1102, 1148
NdeI CATATG 1 cut(s) 1107
NdeII GATC 9 cut(s) 82, 112, 187, 226, 304, 598, 654, 754, 1006
NlaIII CATG 8 cut(s) 140, 176, 197, 201, 239, 302, 987, 1186
NlaIV GGNNCC 2 cut(s) 1119, 1180
NmuCI GTSAC 2 cut(s) 730, 1016
NsiI ATGCAT 1 cut(s) 199
NspI RCATGY 3 cut(s) 197, 302, 987
PagI TCATGA 1 cut(s) 172
PciI ACATGT 1 cut(s) 298
PdmI GAANNNNTTC 2 cut(s) 8, 284
PfeI GAWTC 6 cut(s) 176, 401, 435, 611, 770, 812
PkrI GCNGC 5 cut(s) 325, 397, 446, 571, 583
PleI GAGTC 3 cut(s) 770, 848, 1105
PpsI GAGTC 3 cut(s) 770, 848, 1105
PscI ACATGT 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 663
PspN4I GGNNCC 2 cut(s) 1119, 1180
PspPI GGNCC 1 cut(s) 845
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 581
PsuI RGATCY 5 cut(s) 187, 226, 598, 654, 754
PvuII CAGCTG 2 cut(s) 15, 323
RsaI GTAC 2 cut(s) 701, 1119
RsaNI GTAC 2 cut(s) 700, 1118
SaqAI TTAA 7 cut(s) 51, 345, 368, 426, 1064, 1172, 1216
SatI GCNGC 5 cut(s) 324, 396, 445, 570, 582
Sau3AI GATC 9 cut(s) 82, 112, 187, 226, 304, 598, 654, 754, 1006
Sau96I GGNCC 1 cut(s) 845
ScaI AGTACT 1 cut(s) 701
SchI GAGTC 3 cut(s) 770, 848, 1105
SduI GDGCHC 1 cut(s) 1144
SfaNI GCATC 2 cut(s) 9, 1150
SfcI CTRYAG 1 cut(s) 324
Sse9I AATT 7 cut(s) 120, 150, 481, 676, 818, 1065, 1079
SsiI CCGC 2 cut(s) 251, 1103
SspMI CTAG 2 cut(s) 222, 752
TaaI ACNGT 4 cut(s) 72, 144, 685, 945
TaqI TCGA 3 cut(s) 433, 466, 1212
TasI AATT 7 cut(s) 120, 150, 481, 676, 818, 1065, 1079
TatI WGTACW 1 cut(s) 699
TfiI GAWTC 6 cut(s) 176, 401, 435, 611, 770, 812
Tru1I TTAA 7 cut(s) 51, 345, 368, 426, 1064, 1172, 1216
Tru9I TTAA 7 cut(s) 51, 345, 368, 426, 1064, 1172, 1216
TscAI CASTG 3 cut(s) 508, 739, 745
TseFI GTSAC 2 cut(s) 730, 1016
TseI GCWGC 5 cut(s) 323, 395, 444, 569, 581
Tsp45I GTSAC 2 cut(s) 730, 1016
TspDTI ATGAA 3 cut(s) 17, 189, 309
TspGWI ACGGA 1 cut(s) 1034
TspRI CASTG 3 cut(s) 508, 739, 745
XapI RAATTY 2 cut(s) 676, 818
XceI RCATGY 3 cut(s) 197, 302, 987
XcmI CCANNNNNNNNNTGG 1 cut(s) 126
XmnI GAANNNNTTC 2 cut(s) 8, 284
XspI CTAG 2 cut(s) 222, 752
ZrmI AGTACT 1 cut(s) 701
Zsp2I ATGCAT 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.