Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0013379

Physical Location & Seq

Genomic Coordinates Reverse (-)

20218191 .. 20220350

Gene Structure 2160 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000017704

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1905 bp

ATGAACCAAATCCAATGGACCTTCCAAACCTTTCGCAACGTTTTAAAGACATGCATAGCCGAGAGAGACCTCTTCACAGGCAAAGCCCTCCATGCACTCTACTTCAAGTCCCTCCTTCCTTCTTCCACCTACATTTCCAACCACTTCATCCTCCTCTACTCCAAATGCGGCCGTCTCATCTCCGCCCGGAACGCCTTTGCCGCCACCCAAGACCCCAATGTCTTCTCCTTCAACGCCATCATCGCCGCCTATGCCAAAGAATCACAGACCCGAATTGCACACAACTTGTTCGATGAAATTCCCCAACCGGACTTGGTCTCTTACAACACCCTCATTTCGGCTTATGCTGAAAGGGGAGAGACTGAGCAGGCATTGGGGTTGTTTAGTGGGATGAGAGAAGTGGGTCTTGATGCAGATGGGTTTACTATCTCGGCGGTGATCACGGGTTGTTGTGATGATGTTGGTTTGATTAGGGAGTTGCATTCGTTGATAGTTAAAGGTGGGTTTGATTCGTATGTTTCGGTGAACAATGCGTTGTTAACTTACTATAGCAAGAATGGGTTGTTGGAGGAGGCGAAGAGGGTGTTTAATGTGATGGGAGAAATGAGAGATGAAGTGACATGGAATTCGATGATTGTGGCATATGGGCAGCACCGAAAAGGTTTGAAAGCGCTTGGGTTGTTTCGGGAGATGGTGAGGAGGGGGTTTAATGTGGACATGTTTACATTGGCTAGTGTGTTGACTGCGTTTACTTGCGTGGAGGACTTGTTGGGTGGGCTTCAGTTTCATGCTAAGTTGATCAAAACTGGTTTCCACCAGAATTCTCATGTTGGGAGTGGGCTGATTGATTTGTATTCGAAGTGTGCTGGTGGCATGTCAGAGTGCAGGAAAGTATTTGAAGAGATTCCTGCTCCGGATTTGGTTCTCTGGAACACAATGATTTCTGGATACTCTCAAAATGACGAGTTCTCTGAAGATGCTATTGATTGTTTGCGGCAGATGCAACGTGTTGGTTACTACCCTGATGATTGCAGCTTCGTCTGTGTGATTAGTGCATGCTCTAATTTGTCGTCACCCTTCCAGGGGAAGCAGATACATGCCTTGGCAATCAAATCTGAGATCCCTTCGAATAGAATTTCAGTGAATAATGCTCTAGTTGCAATGTACTCAAAATGCGGGAATCTTCGTGATGCAAGAAGGTTATTTGATAGGATGCCGGAGCATAATACTGTCTCTTTAAATTCGATGATTGCAGGTTATGCACAGCATGGGATTGGAATGGAATCATTGTGCCTTTTCGAACATATGCTTCAGACCGATGTTGTTCCAACAAGTATAACCTTTATCTCTGTCCTTTCTGCTTGTGCTCACTCTGGAAAAGTTGAGGAGGGTCGGAAGTACTTCAGTATGATGCAGGAGAAGTTCAGAATCGAACCAGATGTAGGACATTATTCATGCATGATTGATCTTTTAGGTCGAGCAGGCAAACTGGGTGAAGCTGAGAGGCTCATTGAGGTGATGCCATTCAGCCCTGACACCATTGATAGCTCCCACCCAATGATTAAAGAAATTCATGAATACTTGGAGGAGATGTCAATGAAGATTAAGCGAGCTGGGTATGTGCCTGATGTAAGATGGGCCTTGGTAAAGGATGATGAAACTGTCGAAGGAGAGAAGGAGATAATGTTAGGGCATCACAGTGAAAAGTTAGCAGTTGCATTTGGTTTGATCTCGACAAGAGAAGGGGAGCCTATACTTGTGGTGAAGAACCTGAGGATATGTGGTGATTGCCACAATGCAATCAAGTTTATCTCTGCCATTGCTGGGAGGGAAATCACTGTGAGGGACGCCCACAGGTTTCATTGCTTCAAGGAAGGTCAGTGCTCTTGTGAGGATTATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

635

Amino Acids

70.98

Weight (kDa)

5.92

Isoelectric Point (pI)

37.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_1	PF12854	102 - 132	5.7e-06	PPR repeat
PPR_2	PF13041	103 - 152	2.7e-12	PPR repeat family
PPR	PF01535	106 - 135	1.4e-06	PPR repeat
PPR_2	PF13041	204 - 250	2.1e-09	PPR repeat family
PPR	PF01535	206 - 236	9.4e-09	PPR repeat
PPR_2	PF13041	305 - 355	8.6e-09	PPR repeat family
PPR_2	PF13041	409 - 457	1e-06	PPR repeat family
PPR_1	PF12854	477 - 507	6.2e-06	PPR repeat
DYW_deaminase	PF14432	539 - 634	3.1e-36	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0013379)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49710
fragaria_vesca	FvH4_1g18370
malus_domestica	MD15G1301900.v1.1
prunus_persica	Prupe.6G215200_v2.0.a1
pyrus_communis	pycom02g15520 pycom15g26470
rosa_chinensis	RchiOBHm_Chr2g0109081
rosa_laevigata	RLG00000017704
rosa_multiflora	Rmu_co8217570.1_g000001 Rmu_co8359919.1_g000001 Rmu_sc0003725.1_g000003
rosa_roxburghii	Rroxscaffold_2G00134590
rosa_rugosa	Rorug02G0157000
rosa_samantha	Rh2AG207900 Rh2CG211300 Rh2DG214300
rosa_wichuraiana	Rw0G004070 Rw2G016510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	218
Acc36I	ACCTGC	1 cut(s)	1244
AccIII	TCCGGA	1 cut(s)	913
AciI	CCGC	7 cut(s)	168, 183, 201, 246, 434, 994, 1176
AclI	AACGTT	1 cut(s)	39
AclWI	GGATC	1 cut(s)	1114
AcoI	YGGCCR	1 cut(s)	169
AcsI	RAATTY	6 cut(s)	297, 625, 820, 1134, 1240, 1569
AcuI	CTGAAG	4 cut(s)	764, 993, 1295, 1387
AcyI	GRCGYC	1 cut(s)	1848
AfaI	GTAC	2 cut(s)	1166, 1400
AfeI	AGCGCT	1 cut(s)	672
AfiI	CCNNNNNNNGG	5 cut(s)	337, 1082, 1083, 1442, 1824
AflIII	ACRYGT	2 cut(s)	717, 1006
AgsI	TTSAA	5 cut(s)	106, 232, 667, 899, 1870
AjnI	CCWGG	1 cut(s)	1080
AjuI	GAANNNNNNNTTGG	2 cut(s)	548, 580
AluBI	AGCT	4 cut(s)	1035, 1499, 1548, 1613
AluI	AGCT	4 cut(s)	1035, 1499, 1548, 1613
Alw21I	GWGCWC	2 cut(s)	1369, 1886
Alw26I	GTCTC	5 cut(s)	60, 179, 322, 353, 1237
AlwI	GGATC	1 cut(s)	1114
Aor13HI	TCCGGA	1 cut(s)	913
Aor51HI	AGCGCT	1 cut(s)	672
AoxI	GGCC	2 cut(s)	169, 1638
ApeKI	GCWGC	2 cut(s)	649, 1032
ApoI	RAATTY	6 cut(s)	297, 625, 820, 1134, 1240, 1569
ArsI	GACNNNNNNTTYG	2 cut(s)	610, 642
Asp700I	GAANNNNTTC	2 cut(s)	903, 1400
AspLEI	GCGC	1 cut(s)	673
AspS9I	GGNCC	2 cut(s)	18, 1638
AsuC2I	CCSGG	1 cut(s)	187
AsuHPI	GGTGA	8 cut(s)	448, 535, 706, 1065, 1505, 1528, 1774, 1796
AsuII	TTCGAA	3 cut(s)	857, 1127, 1299
AvaII	GGWCC	1 cut(s)	18
AxyI	CCTNAGG	1 cut(s)	1772
BbsI	GAAGAC	1 cut(s)	214
Bbv12I	GWGCWC	2 cut(s)	1369, 1886
BbvI	GCAGC	2 cut(s)	661, 1044
BccI	CCATC	5 cut(s)	245, 410, 589, 685, 1629
BceAI	ACGGC	1 cut(s)	156
BciT130I	CCWGG	1 cut(s)	1082
BciVI	GTATCC	1 cut(s)	941
BclI	TGATCA	2 cut(s)	438, 798
BcnI	CCSGG	1 cut(s)	187
BcoDI	GTCTC	5 cut(s)	60, 179, 322, 353, 1237
BfaI	CTAG	2 cut(s)	732, 1154
BfmI	CTRYAG	1 cut(s)	547
BfoI	RGCGCY	1 cut(s)	674
BfuAI	ACCTGC	1 cut(s)	1244
BfuI	GTATCC	1 cut(s)	941
BisI	GCNGC	6 cut(s)	169, 201, 246, 650, 995, 1033
BlsI	GCNGC	6 cut(s)	170, 202, 247, 651, 996, 1034
BmcAI	AGTACT	1 cut(s)	1400
Bme1390I	CCNGG	2 cut(s)	187, 1082
Bme18I	GGWCC	1 cut(s)	18
BmgT120I	GGNCC	2 cut(s)	18, 1638
BmiI	GGNNCC	1 cut(s)	1749
BmrFI	CCNGG	2 cut(s)	187, 1082
BmrI	ACTGGG	1 cut(s)	1499
BmsI	GCATC	8 cut(s)	400, 967, 990, 1180, 1203, 1401, 1509, 1702
BmuI	ACTGGG	1 cut(s)	1499
BpiI	GAAGAC	1 cut(s)	214
Bpu14I	TTCGAA	3 cut(s)	857, 1127, 1299
BpuMI	CCSGG	1 cut(s)	187
BsaHI	GRCGYC	1 cut(s)	1848
BsaI	GGTCTC	2 cut(s)	60, 322
BsaJI	CCNNGG	3 cut(s)	1081, 1101, 1641
BsaWI	WCCGGW	2 cut(s)	307, 913
BsaXI	ACNNNNNCTCC	6 cut(s)	752, 782, 1577, 1607, 1670, 1700
Bsc4I	CCNNNNNNNGG	5 cut(s)	337, 1082, 1083, 1442, 1824
Bse1I	ACTGG	2 cut(s)	811, 1494
Bse21I	CCTNAGG	1 cut(s)	1772
Bse3DI	GCAATG	3 cut(s)	1167, 1818, 1861
BseAI	TCCGGA	1 cut(s)	913
BseBI	CCWGG	1 cut(s)	1082
BseDI	CCNNGG	3 cut(s)	1081, 1101, 1641
BseGI	GGATG	4 cut(s)	147, 396, 1218, 1657
BseLI	CCNNNNNNNGG	5 cut(s)	337, 1082, 1083, 1442, 1824
BseMI	GCAATG	3 cut(s)	1167, 1818, 1861
BseMII	CTCAG	4 cut(s)	354, 1107, 1491, 1763
BseNI	ACTGG	2 cut(s)	811, 1494
BseRI	GAGGAG	5 cut(s)	143, 584, 712, 1400, 1601
BseX3I	CGGCCG	1 cut(s)	169
BseXI	GCAGC	2 cut(s)	661, 1044
BseYI	CCCAGC	2 cut(s)	1613, 1823
BsgI	GTGCAG	1 cut(s)	904
Bsh1285I	CGRYCG	1 cut(s)	172
BshFI	GGCC	2 cut(s)	171, 1640
BsiEI	CGRYCG	1 cut(s)	172
BsiHKAI	GWGCWC	2 cut(s)	1369, 1886
BsiSI	CCGG	4 cut(s)	187, 308, 914, 1217
BslFI	GGGAC	2 cut(s)	94, 1859
BslI	CCNNNNNNNGG	5 cut(s)	337, 1082, 1083, 1442, 1824
BsmAI	GTCTC	5 cut(s)	60, 179, 322, 353, 1237
BsmBI	CGTCTC	1 cut(s)	179
BsmFI	GGGAC	2 cut(s)	94, 1859
BsmI	GAATGC	1 cut(s)	481
BsnI	GGCC	2 cut(s)	171, 1640
Bso31I	GGTCTC	2 cut(s)	60, 322
Bsp119I	TTCGAA	3 cut(s)	857, 1127, 1299
Bsp1286I	GDGCHC	2 cut(s)	1369, 1886
Bsp13I	TCCGGA	1 cut(s)	913
Bsp143I	GATC	5 cut(s)	438, 798, 1119, 1465, 1728
BspACI	CCGC	7 cut(s)	168, 183, 201, 246, 434, 994, 1176
BspANI	GGCC	2 cut(s)	171, 1640
BspCNI	CTCAG	4 cut(s)	355, 1108, 1492, 1764
BspEI	TCCGGA	1 cut(s)	913
BspHI	TCATGA	1 cut(s)	1573
BspLI	GGNNCC	1 cut(s)	1749
BspMI	ACCTGC	1 cut(s)	1244
BspPI	GGATC	1 cut(s)	1114
BspT104I	TTCGAA	3 cut(s)	857, 1127, 1299
BspTNI	GGTCTC	2 cut(s)	60, 322
BsrDI	GCAATG	3 cut(s)	1167, 1818, 1861
BsrI	ACTGG	2 cut(s)	811, 1494
BssECI	CCNNGG	3 cut(s)	1081, 1101, 1641
BssMI	GATC	5 cut(s)	438, 798, 1119, 1465, 1728
BssNI	GRCGYC	1 cut(s)	1848
BssT1I	CCWWGG	2 cut(s)	1101, 1641
Bst2UI	CCWGG	1 cut(s)	1082
Bst4CI	ACNGT	4 cut(s)	1231, 1663, 1700, 1840
Bst6I	CTCTTC	3 cut(s)	77, 572, 894
BstACI	GRCGYC	1 cut(s)	1848
BstAPI	GCANNNNNTGC	1 cut(s)	1259
BstBI	TTCGAA	3 cut(s)	857, 1127, 1299
BstC8I	GCNNGC	4 cut(s)	369, 1057, 1483, 1611
BstDEI	CTNAG	5 cut(s)	363, 792, 1116, 1500, 1772
BstF5I	GGATG	4 cut(s)	147, 396, 1218, 1657
BstH2I	RGCGCY	1 cut(s)	674
BstHHI	GCGC	1 cut(s)	673
BstKTI	GATC	5 cut(s)	441, 801, 1122, 1468, 1731
BstMAI	GTCTC	5 cut(s)	60, 179, 322, 353, 1237
BstMBI	GATC	5 cut(s)	438, 798, 1119, 1465, 1728
BstMCI	CGRYCG	1 cut(s)	172
BstMWI	GCNNNNNNNGC	8 cut(s)	92, 191, 200, 242, 251, 1000, 1157, 1259
BstNI	CCWGG	1 cut(s)	1082
BstNSI	RCATGY	5 cut(s)	54, 721, 877, 1059, 1100
BstSCI	CCNGG	2 cut(s)	185, 1080
BstSFI	CTRYAG	1 cut(s)	547
BstV1I	GCAGC	2 cut(s)	661, 1044
BstV2I	GAAGAC	1 cut(s)	214
BstX2I	RGATCY	1 cut(s)	1119
BstYI	RGATCY	1 cut(s)	1119
BstZI	CGGCCG	1 cut(s)	169
Bsu36I	CCTNAGG	1 cut(s)	1772
BsuI	GTATCC	1 cut(s)	941
BsuRI	GGCC	2 cut(s)	171, 1640
BtgZI	GCGATG	1 cut(s)	226
BtsCI	GGATG	4 cut(s)	147, 396, 1218, 1657
BtsIMutI	CAGTG	4 cut(s)	1146, 1705, 1836, 1886
BveI	ACCTGC	1 cut(s)	1244
Cac8I	GCNNGC	4 cut(s)	369, 1057, 1483, 1611
CciI	TCATGA	1 cut(s)	1573
CfoI	GCGC	1 cut(s)	673
Cfr13I	GGNCC	2 cut(s)	18, 1638
CseI	GACGC	1 cut(s)	1856
Csp6I	GTAC	2 cut(s)	1165, 1399
CviQI	GTAC	2 cut(s)	1165, 1399
DdeI	CTNAG	5 cut(s)	363, 792, 1116, 1500, 1772
DpnI	GATC	5 cut(s)	440, 800, 1121, 1467, 1730
DpnII	GATC	5 cut(s)	438, 798, 1119, 1465, 1728
DraI	TTTAAA	2 cut(s)	45, 1239
DrdI	GACNNNNNNGTC	1 cut(s)	218
DseDI	GACNNNNNNGTC	1 cut(s)	218
EaeI	YGGCCR	1 cut(s)	169
EagI	CGGCCG	1 cut(s)	169
Eam1104I	CTCTTC	3 cut(s)	77, 572, 894
EarI	CTCTTC	3 cut(s)	77, 572, 894
EciI	GGCGGA	1 cut(s)	172
EclXI	CGGCCG	1 cut(s)	169
Eco130I	CCWWGG	2 cut(s)	1101, 1641
Eco31I	GGTCTC	2 cut(s)	60, 322
Eco47I	GGWCC	1 cut(s)	18
Eco47III	AGCGCT	1 cut(s)	672
Eco52I	CGGCCG	1 cut(s)	169
Eco57I	CTGAAG	4 cut(s)	764, 993, 1295, 1387
Eco81I	CCTNAGG	1 cut(s)	1772
EcoRI	GAATTC	2 cut(s)	625, 820
EcoRII	CCWGG	1 cut(s)	1080
EcoT14I	CCWWGG	2 cut(s)	1101, 1641
EcoT22I	ATGCAT	2 cut(s)	56, 1460
ErhI	CCWWGG	2 cut(s)	1101, 1641
Esp3I	CGTCTC	1 cut(s)	179
FalI	AAGNNNNNCTT	2 cut(s)	390, 422
FaqI	GGGAC	2 cut(s)	94, 1859
FauI	CCCGC	1 cut(s)	1169
FauNDI	CATATG	2 cut(s)	643, 1305
FbaI	TGATCA	2 cut(s)	438, 798
Fnu4HI	GCNGC	6 cut(s)	169, 201, 246, 650, 995, 1033
FokI	GGATG	4 cut(s)	134, 403, 1225, 1664
Fsp4HI	GCNGC	6 cut(s)	169, 201, 246, 650, 995, 1033
FspBI	CTAG	2 cut(s)	732, 1154
GlaI	GCGC	1 cut(s)	672
GluI	GCNGC	6 cut(s)	169, 201, 246, 650, 995, 1033
GsaI	CCCAGC	2 cut(s)	1617, 1827
HaeII	RGCGCY	1 cut(s)	674
HaeIII	GGCC	2 cut(s)	171, 1640
HapII	CCGG	4 cut(s)	187, 308, 914, 1217
HgaI	GACGC	1 cut(s)	1856
HhaI	GCGC	1 cut(s)	673
Hin1I	GRCGYC	1 cut(s)	1848
Hin6I	GCGC	1 cut(s)	671
HinP1I	GCGC	1 cut(s)	671
HincII	GTYRAC	2 cut(s)	540, 741
HindII	GTYRAC	2 cut(s)	540, 741
HinfI	GANTC	6 cut(s)	260, 509, 904, 1180, 1283, 1428
HpaI	GTTAAC	1 cut(s)	540
HpaII	CCGG	4 cut(s)	187, 308, 914, 1217
HphI	GGTGA	8 cut(s)	448, 535, 706, 1065, 1505, 1528, 1774, 1796
Hpy166II	GTNNAC	7 cut(s)	423, 526, 540, 715, 723, 741, 750
Hpy188I	TCNGA	6 cut(s)	880, 973, 1117, 1314, 1395, 1427
Hpy188III	TCNNGA	9 cut(s)	407, 686, 914, 928, 945, 1187, 1374, 1574, 1732
Hpy8I	GTNNAC	7 cut(s)	423, 526, 540, 715, 723, 741, 750
HpyCH4III	ACNGT	4 cut(s)	1231, 1663, 1700, 1840
HpyCH4IV	ACGT	2 cut(s)	39, 1006
HpyF10VI	GCNNNNNNNGC	8 cut(s)	92, 191, 200, 242, 251, 1000, 1157, 1259
HpyF3I	CTNAG	5 cut(s)	363, 792, 1116, 1500, 1772
HpySE526I	ACGT	2 cut(s)	39, 1006
Hsp92I	GRCGYC	1 cut(s)	1848
HspAI	GCGC	1 cut(s)	671
Kpn2I	TCCGGA	1 cut(s)	913
Ksp22I	TGATCA	2 cut(s)	438, 798
KspAI	GTTAAC	1 cut(s)	540
Kzo9I	GATC	5 cut(s)	438, 798, 1119, 1465, 1728
LmnI	GCTCC	4 cut(s)	916, 1219, 1553, 1747
Lsp1109I	GCAGC	2 cut(s)	661, 1044
LweI	GCATC	8 cut(s)	400, 967, 990, 1180, 1203, 1401, 1509, 1702
MaeI	CTAG	2 cut(s)	732, 1154
MaeII	ACGT	2 cut(s)	39, 1006
MaeIII	GTNAC	3 cut(s)	616, 1013, 1071
MalI	GATC	5 cut(s)	440, 800, 1121, 1467, 1730
MboI	GATC	5 cut(s)	438, 798, 1119, 1465, 1728
MboII	GAAGA	9 cut(s)	64, 114, 214, 589, 911, 986, 1175, 1612, 1777
MflI	RGATCY	1 cut(s)	1119
MhlI	GDGCHC	2 cut(s)	1369, 1886
MluCI	AATT	8 cut(s)	273, 297, 625, 820, 1063, 1134, 1240, 1569
MmeI	TCCRAC	4 cut(s)	162, 546, 1352, 1373
Mph1103I	ATGCAT	2 cut(s)	56, 1460
MroI	TCCGGA	1 cut(s)	913
MroXI	GAANNNNTTC	2 cut(s)	903, 1400
MseI	TTAA	8 cut(s)	44, 495, 539, 588, 708, 1238, 1563, 1605
MslI	CAYNNNNRTG	2 cut(s)	1514, 1698
MspI	CCGG	4 cut(s)	187, 308, 914, 1217
MspR9I	CCNGG	2 cut(s)	187, 1082
Mva1269I	GAATGC	1 cut(s)	481
MvaI	CCWGG	1 cut(s)	1082
MwoI	GCNNNNNNNGC	8 cut(s)	92, 191, 200, 242, 251, 1000, 1157, 1259
NciI	CCSGG	1 cut(s)	187
NdeI	CATATG	2 cut(s)	643, 1305
NdeII	GATC	5 cut(s)	438, 798, 1119, 1465, 1728
NlaIV	GGNNCC	1 cut(s)	1749
NmeAIII	GCCGAG	2 cut(s)	85, 410
NmuCI	GTSAC	2 cut(s)	616, 1071
NsiI	ATGCAT	2 cut(s)	56, 1460
NspI	RCATGY	5 cut(s)	54, 721, 877, 1059, 1100
NspV	TTCGAA	3 cut(s)	857, 1127, 1299
PaeI	GCATGC	1 cut(s)	1059
PagI	TCATGA	1 cut(s)	1573
PciI	ACATGT	1 cut(s)	717
PctI	GAATGC	1 cut(s)	481
PdmI	GAANNNNTTC	2 cut(s)	903, 1400
PfeI	GAWTC	6 cut(s)	260, 509, 904, 1180, 1283, 1428
PflFI	GACNNNGTC	1 cut(s)	314
PkrI	GCNGC	6 cut(s)	170, 202, 247, 651, 996, 1034
PscI	ACATGT	1 cut(s)	717
Psp1406I	AACGTT	1 cut(s)	39
Psp6I	CCWGG	1 cut(s)	1080
PspFI	CCCAGC	2 cut(s)	1613, 1823
PspGI	CCWGG	1 cut(s)	1080
PspN4I	GGNNCC	1 cut(s)	1749
PspPI	GGNCC	2 cut(s)	18, 1638
PsuI	RGATCY	1 cut(s)	1119
PsyI	GACNNNGTC	1 cut(s)	314
RsaI	GTAC	2 cut(s)	1166, 1400
RsaNI	GTAC	2 cut(s)	1165, 1399
RseI	CAYNNNNRTG	2 cut(s)	1514, 1698
SaqAI	TTAA	8 cut(s)	44, 495, 539, 588, 708, 1238, 1563, 1605
SatI	GCNGC	6 cut(s)	169, 201, 246, 650, 995, 1033
Sau3AI	GATC	5 cut(s)	438, 798, 1119, 1465, 1728
Sau96I	GGNCC	2 cut(s)	18, 1638
ScaI	AGTACT	1 cut(s)	1400
ScrFI	CCNGG	2 cut(s)	187, 1082
SduI	GDGCHC	2 cut(s)	1369, 1886
SfaNI	GCATC	8 cut(s)	400, 967, 990, 1180, 1203, 1401, 1509, 1702
SfcI	CTRYAG	1 cut(s)	547
SfuI	TTCGAA	3 cut(s)	857, 1127, 1299
SinI	GGWCC	1 cut(s)	18
SmiMI	CAYNNNNRTG	2 cut(s)	1514, 1698
SphI	GCATGC	1 cut(s)	1059
Sse9I	AATT	8 cut(s)	273, 297, 625, 820, 1063, 1134, 1240, 1569
SsiI	CCGC	7 cut(s)	168, 183, 201, 246, 434, 994, 1176
SspMI	CTAG	2 cut(s)	732, 1154
StyD4I	CCNGG	2 cut(s)	185, 1080
StyI	CCWWGG	2 cut(s)	1101, 1641
TaaI	ACNGT	4 cut(s)	1231, 1663, 1700, 1840
TaiI	ACGT	2 cut(s)	42, 1009
TaqII	GACCGA	1 cut(s)	1331
TasI	AATT	8 cut(s)	273, 297, 625, 820, 1063, 1134, 1240, 1569
TatI	WGTACW	2 cut(s)	1164, 1398
TauI	GCSGC	4 cut(s)	171, 203, 248, 997
TfiI	GAWTC	6 cut(s)	260, 509, 904, 1180, 1283, 1428
Tru1I	TTAA	8 cut(s)	44, 495, 539, 588, 708, 1238, 1563, 1605
Tru9I	TTAA	8 cut(s)	44, 495, 539, 588, 708, 1238, 1563, 1605
TscAI	CASTG	4 cut(s)	1146, 1705, 1843, 1886
TseFI	GTSAC	2 cut(s)	616, 1071
TseI	GCWGC	2 cut(s)	649, 1032
Tsp45I	GTSAC	2 cut(s)	616, 1071
TspRI	CASTG	4 cut(s)	1146, 1705, 1843, 1886
Tth111I	GACNNNGTC	1 cut(s)	314
VpaK11BI	GGWCC	1 cut(s)	18
XapI	RAATTY	6 cut(s)	297, 625, 820, 1134, 1240, 1569
XceI	RCATGY	5 cut(s)	54, 721, 877, 1059, 1100
XmnI	GAANNNNTTC	2 cut(s)	903, 1400
XspI	CTAG	2 cut(s)	732, 1154
ZrmI	AGTACT	1 cut(s)	1400
Zsp2I	ATGCAT	2 cut(s)	56, 1460

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.