Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0013379

Physical Location & Seq

Genomic Coordinates Reverse (-)

19605929 .. 19608088

Gene Structure 2160 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G016510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2160 bp

ATGAACCGAATCCAATGGACCTTGCAAACCTTCCGCAACGTTTTAAAGACATGCATAGCCGAAAGAGACCTCTTCACAGGCAAAGCCCTCCATGCACTATACTTCAAGTCCCTCCTTCCTCCGTCCACCTACATTTCCAACCACTTCATCCTCCTCTACTCCAAATGCGGCCGTCTCATCTCCGCCCGGAACGCCTTTTCCGCCACCCAAGACCCCAATGTCTTCTCCTTCAACGCCATCATGGCCGCCTACGCCAAAGAATCGCAGACCCGAATTGCACACAAGTTGTTCGATGAAATTCCCCAACCGGACTTGGTCTCTTACAACACCCTCATTTCGGCTTATGCTGAAAAGGGAGAGACTGAGCAGGCATTGGGGTTGTTTAGTGGGATGAGAGAAGTGGGTCTTGATGCTGATGGGTTTACCATCTCGGCGGTGATCACGGGTTGTTGTGATGATGTTGGTTTGATTAGGGAGTTGCATTCGTTGATAGTTAAAGGTGGGTTTGATTCGTATGTTTCGGTGAACAATGCGTTATTAACTTACTATAGTAAGAATGGGCTGTTGGAGGAGGCGAAGAGGGTGTTTAATGTGATGGGAGAAATGAGAGATGAAGTGACATGGAATTCGATGATTGTGGCGTATGGGCAGCACCGACAAGGGTTGAAGGCGCTGGGGTTGTTTCAAGACATGGTGAGGAGGGGGTTTGATGTGGACATGTTTACATTGGCTAGTGTGTTGACTGCGTTTACTTGCGTGCAGGACTTGTTGGGTGGGCTTCAGTTTCATGCTAAGTTGATCAAAACCGGTTTCCACCAGAATTCTCATGTTGGGAGTGGGCTGATTGATTTGTATTCAAAGTGTGCTGGTGGCATGTCAGAGTGCTGGAAAGTATTTGAAGAGATTCCTGCTCCGGATTTGGTTCTCTGGAACACAATGATTTCTGGATACTCTCAAAATGACGAGTTCTCTGAAGATGCTATTGATTGTTTGCGGCAGATGCAACGTGTTGGTTACTACCCTGATGATTGCAGCTTCGTCTGTGTGATTAGTGCATGCTCTAATTTGTCGTCACCCTTCCAGGGGAAGCAGATACATGCCTTGGCAATCAAATCTGAGATCCCTTCGAATATAATTTCAGTGGATAATGCTCTAGTTGCAATGTACTCGAAATGTGGGAATCTTCGTGATGCAAGAAGGTTATTTGATAGGATGCCGGAGCATAATACTGTCTCTTTAAATTCGATGATTGCAGGTTATGCACAGCATGGGATTGGAATGGAATCATTGTGCCTTTTCGAACATATGCTTCAGACCGATGTTGTTCCAACAAGTATAACCTTTATCTCTGTCCTTTCTGCTTGTGCTCACTCTGGAAAAGTTGAGGAGGGTCAGAAGTACTTCAGTATGATGCAGGAGAAGTTCAGAATCGAACCAGATGTAGGACATTATTCATGCATGATTGATCTTTTAGGTCGAGCAGGCAAACTGGGTGAAGCTGAGAGGCTCATTGAGGTGATGCCATTCAGCCCTGGCACCATTGGTTGGGCTACCTTACTCAGTGCCTGTAGAACACATGGAAATATTGAACTAGCAGTTAAGGCAGCCAATCAATTGCTTCAGTTGGAACCTTCAAATGCTGTTCCATATGTTATGCTGTCGAATATGTATTCTAGAGCTGGCAAATGGGATGAGGTAGCAAAAATTAGAACGTTGATGCGAGATAGAGGAGTGAAGAAGAAACCAGGTTTAAGTTGGATTGAGGTGAATAAGAGGATACATGTTTTTGTGGCTGAAGATAGCTCCCACCCAATGATTAAAGAAATTCATGAATACTTGGAGGAGATGTCAATGAAGATTAAGCGAGCTGGGTATGTGCCTGATGTAAGATGGGCCTTGGTAAAGGATGATGAAACTGTCGAAGGAGAGAAGGAGATAATGTTAGGGCATCACAGTGAAAAGTTAGCAGTTGCATTTGGTTTGATCTCGACAAGAGAAGGGGAGCCTATACTTGTGGTGAAGAACCTGAGGATATGTGGTGATTGCCACAATGCAATCAAGTTTATCTCTGCTATTGCTGGGAGGGAAATCACTGTGAGGGACGCCCACAGGTTTCATTGCTTCAAGGAAGGGCAGTGCTCTTGTGGGGATTATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

719

Amino Acids

80.35

Weight (kDa)

6.24

Isoelectric Point (pI)

40.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_1	PF12854	102 - 132	3e-06	PPR repeat
PPR_2	PF13041	103 - 152	1.8e-12	PPR repeat family
PPR	PF01535	106 - 135	9e-07	PPR repeat
PPR_2	PF13041	204 - 250	3.9e-09	PPR repeat family
PPR	PF01535	206 - 236	7.5e-08	PPR repeat
PPR_2	PF13041	305 - 355	1e-08	PPR repeat family
PPR_2	PF13041	409 - 457	1.2e-06	PPR repeat family
PPR_1	PF12854	477 - 507	7.2e-06	PPR repeat
E_motif	PF20431	526 - 588	4.9e-22	E motif
Eplus_motif	PF20430	593 - 620	5.1e-06	E+ motif
DYW_deaminase	PF14432	624 - 719	1e-36	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013379)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49710
fragaria_vesca	FvH4_1g18370
malus_domestica	MD15G1301900.v1.1
prunus_persica	Prupe.6G215200_v2.0.a1
pyrus_communis	pycom02g15520 pycom15g26470
rosa_chinensis	RchiOBHm_Chr2g0109081
rosa_laevigata	RLG00000017704
rosa_multiflora	Rmu_co8217570.1_g000001 Rmu_co8359919.1_g000001 Rmu_sc0003725.1_g000003
rosa_roxburghii	Rroxscaffold_2G00134590
rosa_rugosa	Rorug02G0157000
rosa_samantha	Rh2AG207900 Rh2CG211300 Rh2DG214300
rosa_wichuraiana	Rw0G004070 Rw2G016510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	218
Acc36I	ACCTGC	1 cut(s)	1244
AccB1I	GGYRCC	1 cut(s)	1535
AccB7I	CCANNNNNTGG	1 cut(s)	1545
AccIII	TCCGGA	1 cut(s)	913
AciI	CCGC	7 cut(s)	34, 168, 183, 201, 246, 434, 994
AclI	AACGTT	2 cut(s)	39, 1712
AclWI	GGATC	1 cut(s)	1114
AcoI	YGGCCR	2 cut(s)	169, 243
AcsI	RAATTY	5 cut(s)	297, 625, 820, 1240, 1824
AcuI	CTGAAG	6 cut(s)	764, 993, 1295, 1387, 1604, 1815
AcyI	GRCGYC	1 cut(s)	2103
AfaI	GTAC	2 cut(s)	1166, 1400
AfiI	CCNNNNNNNGG	5 cut(s)	337, 1082, 1083, 1442, 1545
AflIII	ACRYGT	3 cut(s)	717, 1006, 1780
AgeI	ACCGGT	1 cut(s)	806
AgsI	TTSAA	9 cut(s)	106, 232, 667, 686, 858, 899, 1589, 1635, 2125
AjnI	CCWGG	3 cut(s)	1080, 1531, 1744
AjuI	GAANNNNNNNTTGG	2 cut(s)	548, 580
AloI	GAACNNNNNNTCC	2 cut(s)	182, 214
AluBI	AGCT	5 cut(s)	1035, 1499, 1679, 1803, 1868
AluI	AGCT	5 cut(s)	1035, 1499, 1679, 1803, 1868
Alw21I	GWGCWC	2 cut(s)	1369, 2141
Alw26I	GTCTC	5 cut(s)	60, 179, 322, 353, 1237
AlwI	GGATC	1 cut(s)	1114
AlwNI	CAGNNNCTG	1 cut(s)	1566
Aor13HI	TCCGGA	1 cut(s)	913
AoxI	GGCC	3 cut(s)	169, 243, 1893
ApeKI	GCWGC	3 cut(s)	649, 1032, 1604
ApoI	RAATTY	5 cut(s)	297, 625, 820, 1240, 1824
ArsI	GACNNNNNNTTYG	2 cut(s)	610, 642
AsiGI	ACCGGT	1 cut(s)	806
Asp700I	GAANNNNTTC	2 cut(s)	903, 1400
AspLEI	GCGC	1 cut(s)	673
AspS9I	GGNCC	2 cut(s)	18, 1893
AsuC2I	CCSGG	1 cut(s)	187
AsuHPI	GGTGA	9 cut(s)	448, 535, 706, 1065, 1505, 1528, 1777, 2029, 2051
AsuII	TTCGAA	2 cut(s)	1127, 1299
AvaII	GGWCC	1 cut(s)	18
AxyI	CCTNAGG	1 cut(s)	2027
BanI	GGYRCC	1 cut(s)	1535
BbsI	GAAGAC	1 cut(s)	214
Bbv12I	GWGCWC	2 cut(s)	1369, 2141
BbvI	GCAGC	3 cut(s)	661, 1044, 1616
BccI	CCATC	5 cut(s)	245, 410, 434, 589, 1884
BceAI	ACGGC	1 cut(s)	156
BcgI	CGANNNNNNTGC	2 cut(s)	1149, 1183
BciT130I	CCWGG	3 cut(s)	1082, 1533, 1746
BciVI	GTATCC	2 cut(s)	941, 1770
BclI	TGATCA	2 cut(s)	438, 798
BcnI	CCSGG	1 cut(s)	187
BcoDI	GTCTC	5 cut(s)	60, 179, 322, 353, 1237
BfaI	CTAG	4 cut(s)	732, 1154, 1592, 1674
BfmI	CTRYAG	2 cut(s)	547, 1567
BfoI	RGCGCY	1 cut(s)	674
BfuAI	ACCTGC	1 cut(s)	1244
BfuI	GTATCC	2 cut(s)	941, 1770
BglI	GCCNNNNNGGC	1 cut(s)	242
BisI	GCNGC	6 cut(s)	169, 246, 650, 995, 1033, 1605
BlsI	GCNGC	6 cut(s)	170, 247, 651, 996, 1034, 1606
BmcAI	AGTACT	1 cut(s)	1400
Bme1390I	CCNGG	4 cut(s)	187, 1082, 1533, 1746
Bme18I	GGWCC	1 cut(s)	18
BmgT120I	GGNCC	2 cut(s)	18, 1893
BmiI	GGNNCC	3 cut(s)	1537, 1629, 2004
BmrFI	CCNGG	4 cut(s)	187, 1082, 1533, 1746
BmrI	ACTGGG	1 cut(s)	1499
BmsI	GCATC	9 cut(s)	400, 967, 990, 1180, 1203, 1401, 1509, 1707, 1957
BmuI	ACTGGG	1 cut(s)	1499
BpiI	GAAGAC	1 cut(s)	214
Bpu14I	TTCGAA	2 cut(s)	1127, 1299
BpuMI	CCSGG	1 cut(s)	187
BsaHI	GRCGYC	1 cut(s)	2103
BsaI	GGTCTC	2 cut(s)	60, 322
BsaJI	CCNNGG	4 cut(s)	1081, 1101, 1531, 1896
BsaWI	WCCGGW	3 cut(s)	307, 806, 913
BsaXI	ACNNNNNCTCC	4 cut(s)	1832, 1862, 1925, 1955
Bsc4I	CCNNNNNNNGG	5 cut(s)	337, 1082, 1083, 1442, 1545
Bse118I	RCCGGY	1 cut(s)	806
Bse1I	ACTGG	1 cut(s)	1494
Bse21I	CCTNAGG	1 cut(s)	2027
Bse3DI	GCAATG	2 cut(s)	1167, 2116
BseAI	TCCGGA	1 cut(s)	913
BseBI	CCWGG	3 cut(s)	1082, 1533, 1746
BseDI	CCNNGG	4 cut(s)	1081, 1101, 1531, 1896
BseGI	GGATG	5 cut(s)	147, 396, 1218, 1696, 1912
BseLI	CCNNNNNNNGG	5 cut(s)	337, 1082, 1083, 1442, 1545
BseMI	GCAATG	2 cut(s)	1167, 2116
BseMII	CTCAG	5 cut(s)	354, 1107, 1491, 1573, 2018
BseNI	ACTGG	1 cut(s)	1494
BseRI	GAGGAG	6 cut(s)	143, 584, 712, 1400, 1743, 1856
BseX3I	CGGCCG	1 cut(s)	169
BseXI	GCAGC	3 cut(s)	661, 1044, 1616
BseYI	CCCAGC	3 cut(s)	673, 1868, 2078
BsgI	GTGCAG	1 cut(s)	779
Bsh1285I	CGRYCG	1 cut(s)	172
BshFI	GGCC	3 cut(s)	171, 245, 1895
BshNI	GGYRCC	1 cut(s)	1535
BshTI	ACCGGT	1 cut(s)	806
BsiEI	CGRYCG	1 cut(s)	172
BsiHKAI	GWGCWC	2 cut(s)	1369, 2141
BsiSI	CCGG	5 cut(s)	187, 308, 807, 914, 1217
BslFI	GGGAC	2 cut(s)	94, 2114
BslI	CCNNNNNNNGG	5 cut(s)	337, 1082, 1083, 1442, 1545
BsmAI	GTCTC	5 cut(s)	60, 179, 322, 353, 1237
BsmBI	CGTCTC	1 cut(s)	179
BsmFI	GGGAC	2 cut(s)	94, 2114
BsmI	GAATGC	1 cut(s)	481
BsnI	GGCC	3 cut(s)	171, 245, 1895
Bso31I	GGTCTC	2 cut(s)	60, 322
Bsp119I	TTCGAA	2 cut(s)	1127, 1299
Bsp1286I	GDGCHC	2 cut(s)	1369, 2141
Bsp13I	TCCGGA	1 cut(s)	913
Bsp143I	GATC	5 cut(s)	438, 798, 1119, 1465, 1983
BspACI	CCGC	7 cut(s)	34, 168, 183, 201, 246, 434, 994
BspANI	GGCC	3 cut(s)	171, 245, 1895
BspCNI	CTCAG	5 cut(s)	355, 1108, 1492, 1572, 2019
BspEI	TCCGGA	1 cut(s)	913
BspHI	TCATGA	1 cut(s)	1828
BspLI	GGNNCC	3 cut(s)	1537, 1629, 2004
BspMI	ACCTGC	1 cut(s)	1244
BspPI	GGATC	1 cut(s)	1114
BspT104I	TTCGAA	2 cut(s)	1127, 1299
BspT107I	GGYRCC	1 cut(s)	1535
BspTNI	GGTCTC	2 cut(s)	60, 322
BsrDI	GCAATG	2 cut(s)	1167, 2116
BsrFI	RCCGGY	1 cut(s)	806
BsrI	ACTGG	1 cut(s)	1494
BssAI	RCCGGY	1 cut(s)	806
BssECI	CCNNGG	4 cut(s)	1081, 1101, 1531, 1896
BssMI	GATC	5 cut(s)	438, 798, 1119, 1465, 1983
BssNI	GRCGYC	1 cut(s)	2103
BssT1I	CCWWGG	2 cut(s)	1101, 1896
Bst2UI	CCWGG	3 cut(s)	1082, 1533, 1746
Bst4CI	ACNGT	4 cut(s)	1231, 1918, 1955, 2095
Bst6I	CTCTTC	3 cut(s)	77, 572, 894
BstACI	GRCGYC	1 cut(s)	2103
BstAPI	GCANNNNNTGC	1 cut(s)	1259
BstBI	TTCGAA	2 cut(s)	1127, 1299
BstC8I	GCNNGC	6 cut(s)	369, 758, 1057, 1483, 1681, 1866
BstDEI	CTNAG	6 cut(s)	363, 792, 1116, 1500, 1559, 2027
BstF5I	GGATG	5 cut(s)	147, 396, 1218, 1696, 1912
BstH2I	RGCGCY	1 cut(s)	674
BstHHI	GCGC	1 cut(s)	673
BstKTI	GATC	5 cut(s)	441, 801, 1122, 1468, 1986
BstMAI	GTCTC	5 cut(s)	60, 179, 322, 353, 1237
BstMBI	GATC	5 cut(s)	438, 798, 1119, 1465, 1983
BstMCI	CGRYCG	1 cut(s)	172
BstMWI	GCNNNNNNNGC	9 cut(s)	92, 191, 200, 242, 251, 1000, 1157, 1259, 1601
BstNI	CCWGG	3 cut(s)	1082, 1533, 1746
BstNSI	RCATGY	6 cut(s)	54, 721, 877, 1059, 1100, 1784
BstSCI	CCNGG	4 cut(s)	185, 1080, 1531, 1744
BstSFI	CTRYAG	2 cut(s)	547, 1567
BstV1I	GCAGC	3 cut(s)	661, 1044, 1616
BstV2I	GAAGAC	1 cut(s)	214
BstX2I	RGATCY	1 cut(s)	1119
BstYI	RGATCY	1 cut(s)	1119
BstZI	CGGCCG	1 cut(s)	169
Bsu36I	CCTNAGG	1 cut(s)	2027
BsuI	GTATCC	2 cut(s)	941, 1770
BsuRI	GGCC	3 cut(s)	171, 245, 1895
BtsCI	GGATG	5 cut(s)	147, 396, 1218, 1696, 1912
BtsI	GCAGTG	1 cut(s)	2141
BtsIMutI	CAGTG	5 cut(s)	1146, 1567, 1960, 2091, 2141
BveI	ACCTGC	1 cut(s)	1244
Cac8I	GCNNGC	6 cut(s)	369, 758, 1057, 1483, 1681, 1866
CaiI	CAGNNNCTG	1 cut(s)	1566
CciI	TCATGA	1 cut(s)	1828
CfoI	GCGC	1 cut(s)	673
Cfr10I	RCCGGY	1 cut(s)	806
Cfr13I	GGNCC	2 cut(s)	18, 1893
CseI	GACGC	1 cut(s)	2111
CsiI	ACCWGGT	1 cut(s)	1744
Csp6I	GTAC	2 cut(s)	1165, 1399
CspAI	ACCGGT	1 cut(s)	806
CviQI	GTAC	2 cut(s)	1165, 1399
DdeI	CTNAG	6 cut(s)	363, 792, 1116, 1500, 1559, 2027
DpnI	GATC	5 cut(s)	440, 800, 1121, 1467, 1985
DpnII	GATC	5 cut(s)	438, 798, 1119, 1465, 1983
DraI	TTTAAA	2 cut(s)	45, 1239
DrdI	GACNNNNNNGTC	1 cut(s)	218
DseDI	GACNNNNNNGTC	1 cut(s)	218
EaeI	YGGCCR	2 cut(s)	169, 243
EagI	CGGCCG	1 cut(s)	169
Eam1104I	CTCTTC	3 cut(s)	77, 572, 894
EarI	CTCTTC	3 cut(s)	77, 572, 894
EciI	GGCGGA	2 cut(s)	172, 190
EclXI	CGGCCG	1 cut(s)	169
Eco130I	CCWWGG	2 cut(s)	1101, 1896
Eco31I	GGTCTC	2 cut(s)	60, 322
Eco47I	GGWCC	1 cut(s)	18
Eco52I	CGGCCG	1 cut(s)	169
Eco57I	CTGAAG	6 cut(s)	764, 993, 1295, 1387, 1604, 1815
Eco81I	CCTNAGG	1 cut(s)	2027
EcoRI	GAATTC	2 cut(s)	625, 820
EcoRII	CCWGG	3 cut(s)	1080, 1531, 1744
EcoT14I	CCWWGG	2 cut(s)	1101, 1896
EcoT22I	ATGCAT	2 cut(s)	56, 1460
ErhI	CCWWGG	2 cut(s)	1101, 1896
Esp3I	CGTCTC	1 cut(s)	179
FalI	AAGNNNNNCTT	2 cut(s)	390, 422
FaqI	GGGAC	2 cut(s)	94, 2114
FauNDI	CATATG	2 cut(s)	1305, 1648
FbaI	TGATCA	2 cut(s)	438, 798
Fnu4HI	GCNGC	6 cut(s)	169, 246, 650, 995, 1033, 1605
FokI	GGATG	5 cut(s)	134, 403, 1225, 1703, 1919
Fsp4HI	GCNGC	6 cut(s)	169, 246, 650, 995, 1033, 1605
FspBI	CTAG	4 cut(s)	732, 1154, 1592, 1674
GlaI	GCGC	1 cut(s)	672
GluI	GCNGC	6 cut(s)	169, 246, 650, 995, 1033, 1605
GsaI	CCCAGC	3 cut(s)	677, 1872, 2082
HaeII	RGCGCY	1 cut(s)	674
HaeIII	GGCC	3 cut(s)	171, 245, 1895
HapII	CCGG	5 cut(s)	187, 308, 807, 914, 1217
HgaI	GACGC	1 cut(s)	2111
HhaI	GCGC	1 cut(s)	673
Hin1I	GRCGYC	1 cut(s)	2103
Hin6I	GCGC	1 cut(s)	671
HinP1I	GCGC	1 cut(s)	671
HincII	GTYRAC	1 cut(s)	741
HindII	GTYRAC	1 cut(s)	741
HinfI	GANTC	7 cut(s)	9, 260, 509, 904, 1180, 1283, 1428
HpaII	CCGG	5 cut(s)	187, 308, 807, 914, 1217
HphI	GGTGA	9 cut(s)	448, 535, 706, 1065, 1505, 1528, 1777, 2029, 2051
Hpy166II	GTNNAC	7 cut(s)	126, 423, 526, 715, 723, 741, 750
Hpy188I	TCNGA	6 cut(s)	880, 973, 1117, 1314, 1395, 1427
Hpy8I	GTNNAC	7 cut(s)	126, 423, 526, 715, 723, 741, 750
HpyCH4III	ACNGT	4 cut(s)	1231, 1918, 1955, 2095
HpyCH4IV	ACGT	3 cut(s)	39, 1006, 1712
HpyF10VI	GCNNNNNNNGC	9 cut(s)	92, 191, 200, 242, 251, 1000, 1157, 1259, 1601
HpyF3I	CTNAG	6 cut(s)	363, 792, 1116, 1500, 1559, 2027
HpySE526I	ACGT	3 cut(s)	39, 1006, 1712
Hsp92I	GRCGYC	1 cut(s)	2103
HspAI	GCGC	1 cut(s)	671
Kpn2I	TCCGGA	1 cut(s)	913
Ksp22I	TGATCA	2 cut(s)	438, 798
Kzo9I	GATC	5 cut(s)	438, 798, 1119, 1465, 1983
LmnI	GCTCC	4 cut(s)	916, 1219, 1808, 2002
Lsp1109I	GCAGC	3 cut(s)	661, 1044, 1616
LweI	GCATC	9 cut(s)	400, 967, 990, 1180, 1203, 1401, 1509, 1707, 1957
MabI	ACCWGGT	1 cut(s)	1744
MaeI	CTAG	4 cut(s)	732, 1154, 1592, 1674
MaeII	ACGT	3 cut(s)	39, 1006, 1712
MaeIII	GTNAC	3 cut(s)	616, 1013, 1071
MalI	GATC	5 cut(s)	440, 800, 1121, 1467, 1985
MboI	GATC	5 cut(s)	438, 798, 1119, 1465, 1983
MfeI	CAATTG	1 cut(s)	1613
MflI	RGATCY	1 cut(s)	1119
MhlI	GDGCHC	2 cut(s)	1369, 2141
MmeI	TCCRAC	5 cut(s)	162, 546, 1352, 1605, 1736
Mph1103I	ATGCAT	2 cut(s)	56, 1460
MroI	TCCGGA	1 cut(s)	913
MroXI	GAANNNNTTC	2 cut(s)	903, 1400
MseI	TTAA	9 cut(s)	44, 495, 539, 588, 1238, 1599, 1751, 1818, 1860
MslI	CAYNNNNRTG	2 cut(s)	1514, 1953
MspI	CCGG	5 cut(s)	187, 308, 807, 914, 1217
MspR9I	CCNGG	4 cut(s)	187, 1082, 1533, 1746
MunI	CAATTG	1 cut(s)	1613
Mva1269I	GAATGC	1 cut(s)	481
MvaI	CCWGG	3 cut(s)	1082, 1533, 1746
MwoI	GCNNNNNNNGC	9 cut(s)	92, 191, 200, 242, 251, 1000, 1157, 1259, 1601
NciI	CCSGG	1 cut(s)	187
NdeI	CATATG	2 cut(s)	1305, 1648
NdeII	GATC	5 cut(s)	438, 798, 1119, 1465, 1983
NlaIV	GGNNCC	3 cut(s)	1537, 1629, 2004
NmeAIII	GCCGAG	1 cut(s)	410
NmuCI	GTSAC	2 cut(s)	616, 1071
NsiI	ATGCAT	2 cut(s)	56, 1460
NspI	RCATGY	6 cut(s)	54, 721, 877, 1059, 1100, 1784
NspV	TTCGAA	2 cut(s)	1127, 1299
PaeI	GCATGC	1 cut(s)	1059
PagI	TCATGA	1 cut(s)	1828
PciI	ACATGT	2 cut(s)	717, 1780
PctI	GAATGC	1 cut(s)	481
PdmI	GAANNNNTTC	2 cut(s)	903, 1400
PfeI	GAWTC	7 cut(s)	9, 260, 509, 904, 1180, 1283, 1428
PflFI	GACNNNGTC	1 cut(s)	314
PflMI	CCANNNNNTGG	1 cut(s)	1545
PinAI	ACCGGT	1 cut(s)	806
PkrI	GCNGC	6 cut(s)	170, 247, 651, 996, 1034, 1606
PscI	ACATGT	2 cut(s)	717, 1780
Psp1406I	AACGTT	2 cut(s)	39, 1712
Psp6I	CCWGG	3 cut(s)	1080, 1531, 1744
PspFI	CCCAGC	3 cut(s)	673, 1868, 2078
PspGI	CCWGG	3 cut(s)	1080, 1531, 1744
PspN4I	GGNNCC	3 cut(s)	1537, 1629, 2004
PspPI	GGNCC	2 cut(s)	18, 1893
PstNI	CAGNNNCTG	1 cut(s)	1566
PsuI	RGATCY	1 cut(s)	1119
PsyI	GACNNNGTC	1 cut(s)	314
RsaI	GTAC	2 cut(s)	1166, 1400
RsaNI	GTAC	2 cut(s)	1165, 1399
RseI	CAYNNNNRTG	2 cut(s)	1514, 1953
SaqAI	TTAA	9 cut(s)	44, 495, 539, 588, 1238, 1599, 1751, 1818, 1860
SatI	GCNGC	6 cut(s)	169, 246, 650, 995, 1033, 1605
Sau3AI	GATC	5 cut(s)	438, 798, 1119, 1465, 1983
Sau96I	GGNCC	2 cut(s)	18, 1893
ScaI	AGTACT	1 cut(s)	1400
ScrFI	CCNGG	4 cut(s)	187, 1082, 1533, 1746
SduI	GDGCHC	2 cut(s)	1369, 2141
SexAI	ACCWGGT	1 cut(s)	1744
SfaNI	GCATC	9 cut(s)	400, 967, 990, 1180, 1203, 1401, 1509, 1707, 1957
SfcI	CTRYAG	2 cut(s)	547, 1567
SfuI	TTCGAA	2 cut(s)	1127, 1299
SinI	GGWCC	1 cut(s)	18
SmiMI	CAYNNNNRTG	2 cut(s)	1514, 1953
SphI	GCATGC	1 cut(s)	1059
SsiI	CCGC	7 cut(s)	34, 168, 183, 201, 246, 434, 994
SspI	AATATT	1 cut(s)	1585
SspMI	CTAG	4 cut(s)	732, 1154, 1592, 1674
StyD4I	CCNGG	4 cut(s)	185, 1080, 1531, 1744
StyI	CCWWGG	2 cut(s)	1101, 1896
TaaI	ACNGT	4 cut(s)	1231, 1918, 1955, 2095
TaiI	ACGT	3 cut(s)	42, 1009, 1715
TaqII	GACCGA	1 cut(s)	1331
TatI	WGTACW	2 cut(s)	1164, 1398
TauI	GCSGC	3 cut(s)	171, 248, 997
TfiI	GAWTC	7 cut(s)	9, 260, 509, 904, 1180, 1283, 1428
Tru1I	TTAA	9 cut(s)	44, 495, 539, 588, 1238, 1599, 1751, 1818, 1860
Tru9I	TTAA	9 cut(s)	44, 495, 539, 588, 1238, 1599, 1751, 1818, 1860
TscAI	CASTG	5 cut(s)	1146, 1567, 1960, 2098, 2141
TseFI	GTSAC	2 cut(s)	616, 1071
TseI	GCWGC	3 cut(s)	649, 1032, 1604
Tsp45I	GTSAC	2 cut(s)	616, 1071
TspGWI	ACGGA	1 cut(s)	111
TspRI	CASTG	5 cut(s)	1146, 1567, 1960, 2098, 2141
Tth111I	GACNNNGTC	1 cut(s)	314
Van91I	CCANNNNNTGG	1 cut(s)	1545
VpaK11BI	GGWCC	1 cut(s)	18
XapI	RAATTY	5 cut(s)	297, 625, 820, 1240, 1824
XbaI	TCTAGA	1 cut(s)	1673
XceI	RCATGY	6 cut(s)	54, 721, 877, 1059, 1100, 1784
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1752
XmnI	GAANNNNTTC	2 cut(s)	903, 1400
XspI	CTAG	4 cut(s)	732, 1154, 1592, 1674
ZrmI	AGTACT	1 cut(s)	1400
Zsp2I	ATGCAT	2 cut(s)	56, 1460

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.