RLG00000018346

1-acyl-sn-glycerol-3-phosphate acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013710
Physical Location & Seq
Forward (+)
28577782 .. 28578924
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018346

Sequence Viewer

Length: 1143 bp
ATGGCTCATATCATAGAGGACACAGTGCATCTTTCATTTATGCTCTTTTTTGTCATCTCAGGCCTTGTGGTGAATCTCATTCAAGCCATTTTCTTCGTCCTGGTTAGGCCTTTATCTAAGAATATATACAGAAGGATAAACGGAGTAGTGGCAGAATTGTTATGGCTGGAGGTGGTGTGGCTCGTGGAGTGGGGGGCAGGCCTTGAGATTCAAGTACACACAGATAATGACCCCAAAACAATGACCAGCGTGATGGGTAAAGAAAATGCCATTGTCGTTTCTAATTACGGAAATGATATCAATTTCATTCCGGTCCTGGCTCAGCGCTCTGGTTGCCTCTGCAGCTCATTATTTAGTGTTAACGATAAATTCACCAAGTTCCTTCCCGTCTTTGGTTGGGCGATGTGGTTCTCTGAAAATATCTTTATGGAGAGTAACTATTGGGGTCCGAACCAAGATAAAGCCTCGGCATATATATCAGGTCTCCGTGGACTCAAGAACTTCCCTCGGCCTATATGGATGACTCTATGTTCAGAAGAAACAGGGGGAGGTGGGATGAGAAGTGAGGCCTTTGTCTCTGTAGTGAAACACATGAGGTCTTTTGTTCCAGCCGTGTATGATGTAACAATCGCTTTCCCTGAAGTTAGTTCAGATTCACCTCAAACACTGTTACAACTCTTCTGCGAGAGGCTGAGGCGACGAAAGTCATATGTGGTGCATGTGCATGTTAAGCGGCATGAGATTAGGGGTTTGCCTCGTAAACATGGTGCCCTTGAGAAATGGTGTGAGGGTGTGTTGGAGGCCAAGCAAGCCTTATTGGACCAACACAGAGCGGACCAAACTTTTGGTGACCAAGTACAAGTACTAGAAACGTGCTCTGGTAAACGCCGGCCACTCAAGCCTCTTTTGGTTGTTACCTCTTGGGCACTTGTATTTGTATTCTCGGCAATCAAGTTCCCCCTCTTATCTTTACTAGTGCTACTCATTGCATCCTCAGGCCTGACTTACTTTCTCAATCGGGGCCGCTTGTCTTCCCTACTGCTGAATCTTACAGCCTTCCTTCTTAGACGTGCTTTTGGTTCTCAAGTTGCATCACTTCTCCTCATTAGTGCTTCTTATTACATTTACTTCCTTCCTTCTTAG

Protein Analysis

381

Amino Acids

42.95

Weight (kDa)

9.27

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransf_C PF16076 232 - 308 6.9e-12 Acyltransferase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 767
AccBSI CCGCTC 1 cut(s) 833
AciI CCGC 3 cut(s) 733, 833, 1024
AcoI YGGCCR 1 cut(s) 890
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 1 cut(s) 660
AfaI GTAC 3 cut(s) 216, 858, 864
AfeI AGCGCT 1 cut(s) 326
AfiI CCNNNNNNNGG 1 cut(s) 392
AgsI TTSAA 2 cut(s) 83, 212
AhlI ACTAGT 1 cut(s) 973
AjiI CACGTC 1 cut(s) 1070
AjnI CCWGG 2 cut(s) 99, 315
AluBI AGCT 1 cut(s) 345
AluI AGCT 1 cut(s) 345
Alw21I GWGCWC 1 cut(s) 878
Alw26I GTCTC 2 cut(s) 488, 580
Aor51HI AGCGCT 1 cut(s) 326
AoxI GGCC 9 cut(s) 61, 107, 199, 509, 567, 801, 890, 997, 1021
ApeKI GCWGC 1 cut(s) 342
ApoI RAATTY 1 cut(s) 368
ArsI GACNNNNNNTTYG 2 cut(s) 827, 859
AspLEI GCGC 1 cut(s) 327
AspS9I GGNCC 5 cut(s) 313, 446, 820, 835, 1021
AsuHPI GGTGA 4 cut(s) 82, 364, 648, 860
AvaII GGWCC 4 cut(s) 313, 446, 820, 835
AxyI CCTNAGG 1 cut(s) 994
BaeGI GKGCMC 2 cut(s) 772, 928
BanI GGYRCC 1 cut(s) 767
BauI CACGAG 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 1023
Bbv12I GWGCWC 1 cut(s) 878
BbvCI CCTCAGC 1 cut(s) 692
BbvI GCAGC 1 cut(s) 354
BccI CCATC 1 cut(s) 247
BceAI ACGGC 1 cut(s) 596
BciT130I CCWGG 2 cut(s) 101, 317
BcoDI GTCTC 2 cut(s) 488, 580
BcuI ACTAGT 1 cut(s) 973
BfaI CTAG 2 cut(s) 866, 974
BfmI CTRYAG 2 cut(s) 340, 579
BfoI RGCGCY 1 cut(s) 328
BisI GCNGC 3 cut(s) 343, 734, 1024
BlpI GCTNAGC 1 cut(s) 321
BlsI GCNGC 3 cut(s) 344, 735, 1025
BmcAI AGTACT 1 cut(s) 864
Bme1390I CCNGG 2 cut(s) 101, 317
Bme18I GGWCC 4 cut(s) 313, 446, 820, 835
BmgBI CACGTC 1 cut(s) 1070
BmgT120I GGNCC 5 cut(s) 313, 446, 820, 835, 1021
BmiI GGNNCC 3 cut(s) 447, 769, 1022
BmrFI CCNGG 2 cut(s) 101, 317
BmsI GCATC 3 cut(s) 37, 998, 1100
BoxI GACNNNNGTC 1 cut(s) 703
BpiI GAAGAC 1 cut(s) 1023
BpmI CTGGAG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 692
Bpu1102I GCTNAGC 1 cut(s) 321
BpuEI CTTGAG 5 cut(s) 224, 479, 794, 881, 1068
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 3 cut(s) 465, 487, 506
BsaWI WCCGGW 1 cut(s) 310
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 392
Bse118I RCCGGY 1 cut(s) 888
Bse21I CCTNAGG 1 cut(s) 994
Bse3DI GCAATG 1 cut(s) 984
BseBI CCWGG 2 cut(s) 101, 317
BseDI CCNNGG 3 cut(s) 465, 487, 506
BseGI GGATG 3 cut(s) 525, 561, 989
BseLI CCNNNNNNNGG 1 cut(s) 392
BseMI GCAATG 1 cut(s) 984
BseMII CTCAG 4 cut(s) 72, 335, 683, 1008
BseRI GAGGAG 1 cut(s) 1091
BseSI GKGCMC 2 cut(s) 772, 928
BseXI GCAGC 1 cut(s) 354
BshFI GGCC 9 cut(s) 63, 109, 201, 511, 569, 803, 892, 999, 1023
BshNI GGYRCC 1 cut(s) 767
BsiHKAI GWGCWC 1 cut(s) 878
BsiSI CCGG 2 cut(s) 311, 889
BslI CCNNNNNNNGG 1 cut(s) 392
BsmAI GTCTC 2 cut(s) 488, 580
BsnI GGCC 9 cut(s) 63, 109, 201, 511, 569, 803, 892, 999, 1023
Bso31I GGTCTC 1 cut(s) 488
Bsp1286I GDGCHC 3 cut(s) 772, 878, 928
Bsp1720I GCTNAGC 1 cut(s) 321
BspACI CCGC 3 cut(s) 733, 833, 1024
BspANI GGCC 9 cut(s) 63, 109, 201, 511, 569, 803, 892, 999, 1023
BspCNI CTCAG 4 cut(s) 71, 334, 684, 1007
BspLI GGNNCC 3 cut(s) 447, 769, 1022
BspMAI CTGCAG 1 cut(s) 344
BspT107I GGYRCC 1 cut(s) 767
BspTNI GGTCTC 1 cut(s) 488
BsrBI CCGCTC 1 cut(s) 833
BsrDI GCAATG 1 cut(s) 984
BsrFI RCCGGY 1 cut(s) 888
BssAI RCCGGY 1 cut(s) 888
BssECI CCNNGG 3 cut(s) 465, 487, 506
BssSI CACGAG 1 cut(s) 182
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 2 cut(s) 101, 317
Bst4CI ACNGT 2 cut(s) 25, 669
Bst6I CTCTTC 1 cut(s) 683
BstC8I GCNNGC 3 cut(s) 199, 810, 890
BstDEI CTNAG 7 cut(s) 58, 117, 321, 692, 994, 1064, 1140
BstDSI CCRYGG 1 cut(s) 487
BstEII GGTNACC 1 cut(s) 848
BstF5I GGATG 3 cut(s) 525, 561, 989
BstH2I RGCGCY 1 cut(s) 328
BstHHI GCGC 1 cut(s) 327
BstMAI GTCTC 2 cut(s) 488, 580
BstMWI GCNNNNNNNGC 5 cut(s) 333, 342, 730, 809, 898
BstNI CCWGG 2 cut(s) 101, 317
BstNSI RCATGY 2 cut(s) 722, 728
BstPAI GACNNNNGTC 1 cut(s) 703
BstPI GGTNACC 1 cut(s) 848
BstSCI CCNGG 2 cut(s) 99, 315
BstSFI CTRYAG 2 cut(s) 340, 579
BstSLI GKGCMC 2 cut(s) 772, 928
BstV1I GCAGC 1 cut(s) 354
BstV2I GAAGAC 1 cut(s) 1023
BstXI CCANNNNNNTGG 2 cut(s) 253, 845
Bsu36I CCTNAGG 1 cut(s) 994
BsuRI GGCC 9 cut(s) 63, 109, 201, 511, 569, 803, 892, 999, 1023
BtgI CCRYGG 1 cut(s) 487
BtgZI GCGATG 1 cut(s) 416
BtrI CACGTC 1 cut(s) 1070
BtsCI GGATG 3 cut(s) 525, 561, 989
BtsIMutI CAGTG 2 cut(s) 30, 665
Cac8I GCNNGC 3 cut(s) 199, 810, 890
CfoI GCGC 1 cut(s) 327
Cfr10I RCCGGY 1 cut(s) 888
Cfr13I GGNCC 5 cut(s) 313, 446, 820, 835, 1021
Csp6I GTAC 3 cut(s) 215, 857, 863
CviAII CATG 5 cut(s) 592, 719, 725, 737, 764
CviQI GTAC 3 cut(s) 215, 857, 863
DdeI CTNAG 7 cut(s) 58, 117, 321, 692, 994, 1064, 1140
EaeI YGGCCR 1 cut(s) 890
Eam1104I CTCTTC 1 cut(s) 683
EarI CTCTTC 1 cut(s) 683
Eco147I AGGCCT 5 cut(s) 63, 109, 201, 569, 999
Eco31I GGTCTC 1 cut(s) 488
Eco32I GATATC 1 cut(s) 298
Eco47I GGWCC 4 cut(s) 313, 446, 820, 835
Eco47III AGCGCT 1 cut(s) 326
Eco57I CTGAAG 1 cut(s) 660
Eco81I CCTNAGG 1 cut(s) 994
Eco91I GGTNACC 1 cut(s) 848
EcoO65I GGTNACC 1 cut(s) 848
EcoRII CCWGG 2 cut(s) 99, 315
EcoRV GATATC 1 cut(s) 298
FaeI CATG 5 cut(s) 595, 722, 728, 740, 767
FalI AAGNNNNNCTT 2 cut(s) 797, 829
FatI CATG 5 cut(s) 591, 718, 724, 736, 763
FauNDI CATATG 1 cut(s) 709
Fnu4HI GCNGC 3 cut(s) 343, 734, 1024
FokI GGATG 3 cut(s) 532, 568, 976
Fsp4HI GCNGC 3 cut(s) 343, 734, 1024
FspBI CTAG 2 cut(s) 866, 974
GlaI GCGC 1 cut(s) 326
GluI GCNGC 3 cut(s) 343, 734, 1024
GsuI CTGGAG 1 cut(s) 188
HaeII RGCGCY 1 cut(s) 328
HaeIII GGCC 9 cut(s) 63, 109, 201, 511, 569, 803, 892, 999, 1023
HapII CCGG 2 cut(s) 311, 889
HhaI GCGC 1 cut(s) 327
Hin1II CATG 5 cut(s) 595, 722, 728, 740, 767
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 6 cut(s) 73, 208, 492, 523, 653, 1045
HpaI GTTAAC 1 cut(s) 361
HpaII CCGG 2 cut(s) 311, 889
HphI GGTGA 4 cut(s) 82, 364, 648, 860
Hpy166II GTNNAC 5 cut(s) 217, 361, 491, 761, 884
Hpy188I TCNGA 4 cut(s) 415, 450, 535, 652
Hpy188III TCNNGA 1 cut(s) 496
Hpy8I GTNNAC 5 cut(s) 217, 361, 491, 761, 884
Hpy99I CGWCG 1 cut(s) 702
HpyAV CCTTC 5 cut(s) 126, 392, 1066, 1070, 1142
HpyCH4III ACNGT 2 cut(s) 25, 669
HpyCH4IV ACGT 2 cut(s) 872, 1069
HpyCH4V TGCA 6 cut(s) 28, 342, 718, 724, 989, 1091
HpyF10VI GCNNNNNNNGC 5 cut(s) 333, 342, 730, 809, 898
HpyF3I CTNAG 7 cut(s) 58, 117, 321, 692, 994, 1064, 1140
HpySE526I ACGT 2 cut(s) 872, 1069
Hsp92II CATG 5 cut(s) 595, 722, 728, 740, 767
HspAI GCGC 1 cut(s) 325
KroI GCCGGC 1 cut(s) 888
KroNI GCCGGC 1 cut(s) 890
KspAI GTTAAC 1 cut(s) 361
Lsp1109I GCAGC 1 cut(s) 354
LweI GCATC 3 cut(s) 37, 998, 1100
MaeI CTAG 2 cut(s) 866, 974
MaeII ACGT 2 cut(s) 872, 1069
MaeIII GTNAC 5 cut(s) 434, 622, 669, 848, 913
MbiI CCGCTC 1 cut(s) 833
MboII GAAGA 4 cut(s) 85, 548, 670, 1023
MhlI GDGCHC 3 cut(s) 772, 878, 928
MluCI AATT 4 cut(s) 155, 283, 301, 368
MlyI GAGTC 2 cut(s) 486, 517
MmeI TCCRAC 1 cut(s) 777
MroNI GCCGGC 1 cut(s) 888
MseI TTAA 2 cut(s) 360, 729
MslI CAYNNNNRTG 1 cut(s) 723
MspI CCGG 2 cut(s) 311, 889
MspR9I CCNGG 2 cut(s) 101, 317
MvaI CCWGG 2 cut(s) 101, 317
MwoI GCNNNNNNNGC 5 cut(s) 333, 342, 730, 809, 898
NaeI GCCGGC 1 cut(s) 890
NdeI CATATG 1 cut(s) 709
NgoMIV GCCGGC 1 cut(s) 888
NlaIII CATG 5 cut(s) 595, 722, 728, 740, 767
NlaIV GGNNCC 3 cut(s) 447, 769, 1022
NmeAIII GCCGAG 3 cut(s) 446, 487, 923
NmuCI GTSAC 1 cut(s) 848
NspI RCATGY 2 cut(s) 722, 728
PceI AGGCCT 5 cut(s) 63, 109, 201, 569, 999
PdiI GCCGGC 1 cut(s) 890
PfeI GAWTC 4 cut(s) 73, 208, 653, 1045
PkrI GCNGC 3 cut(s) 344, 735, 1025
PleI GAGTC 2 cut(s) 486, 517
PpsI GAGTC 2 cut(s) 486, 517
PshAI GACNNNNGTC 1 cut(s) 703
Psp6I CCWGG 2 cut(s) 99, 315
PspEI GGTNACC 1 cut(s) 848
PspGI CCWGG 2 cut(s) 99, 315
PspN4I GGNNCC 3 cut(s) 447, 769, 1022
PspPI GGNCC 5 cut(s) 313, 446, 820, 835, 1021
PstI CTGCAG 1 cut(s) 344
RsaI GTAC 3 cut(s) 216, 858, 864
RsaNI GTAC 3 cut(s) 215, 857, 863
RseI CAYNNNNRTG 1 cut(s) 723
SaqAI TTAA 2 cut(s) 360, 729
SatI GCNGC 3 cut(s) 343, 734, 1024
Sau96I GGNCC 5 cut(s) 313, 446, 820, 835, 1021
ScaI AGTACT 1 cut(s) 864
SchI GAGTC 2 cut(s) 486, 517
ScrFI CCNGG 2 cut(s) 101, 317
SduI GDGCHC 3 cut(s) 772, 878, 928
SetI ASST 9 cut(s) 174, 347, 484, 553, 599, 661, 875, 920, 1072
SfaNI GCATC 3 cut(s) 37, 998, 1100
SfcI CTRYAG 2 cut(s) 340, 579
SinI GGWCC 4 cut(s) 313, 446, 820, 835
SmiMI CAYNNNNRTG 1 cut(s) 723
SmlI CTYRAG 5 cut(s) 203, 494, 773, 896, 1083
SmoI CTYRAG 5 cut(s) 203, 494, 773, 896, 1083
SpeI ACTAGT 1 cut(s) 973
Sse9I AATT 4 cut(s) 155, 283, 301, 368
SseBI AGGCCT 5 cut(s) 63, 109, 201, 569, 999
SsiI CCGC 3 cut(s) 733, 833, 1024
SspMI CTAG 2 cut(s) 866, 974
StuI AGGCCT 5 cut(s) 63, 109, 201, 569, 999
StyD4I CCNGG 2 cut(s) 99, 315
TaaI ACNGT 2 cut(s) 25, 669
TaiI ACGT 2 cut(s) 875, 1072
TasI AATT 4 cut(s) 155, 283, 301, 368
TatI WGTACW 3 cut(s) 214, 856, 862
TauI GCSGC 2 cut(s) 736, 1026
TfiI GAWTC 4 cut(s) 73, 208, 653, 1045
Tru1I TTAA 2 cut(s) 360, 729
Tru9I TTAA 2 cut(s) 360, 729
TscAI CASTG 2 cut(s) 30, 672
TseFI GTSAC 1 cut(s) 848
TseI GCWGC 1 cut(s) 342
Tsp45I GTSAC 1 cut(s) 848
TspDTI ATGAA 2 cut(s) 24, 295
TspGWI ACGGA 3 cut(s) 156, 303, 476
TspRI CASTG 2 cut(s) 30, 672
VpaK11BI GGWCC 4 cut(s) 313, 446, 820, 835
XapI RAATTY 1 cut(s) 368
XceI RCATGY 2 cut(s) 722, 728
XspI CTAG 2 cut(s) 866, 974
ZrmI AGTACT 1 cut(s) 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.