Rroxscaffold_2G00126530

1-acyl-sn-glycerol-3-phosphate acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013710
Physical Location & Seq
Reverse (-)
61861879 .. 61864444
2566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00126530.1

Sequence Viewer

Length: 1248 bp
ATGGCTCATATCATAGAGGACACAGTGCATCTTTCATTTATGCTCTTTTTTGTCATTTCAGGCCTTGTGGTGAATCTCATTCAAGCCATTTTCTTCGTCCTGATTCGGCCTTTATCTAAGAATATATACAGAAGGATAAACGGAGTAGTGGCAGAATTGTTATGGCTGGAGGTGGTGTGGCTCGTGGAGTGGGGGGCAGGCCTTGAGATTCAAGTACACACAGATAATGACCCCAAAACAATGACCGGCGTGATGGGTAAAGAAAATGCCATTGTCGTTTCTAATTACGGAAATGATATCAATTTCATCCCGGTTCTGGCTCAGCGCTCTGGTTGCCTCTGCAGCTCATTATTTAGTGTTAACGATAAATTCACCAAGTTCCTTCCTGTTATTGGTTGGGCGATGTGGTTCTCTGAAAATATCTTTATGGAGAGTAACTATTGGGGTCCGAACCAAGACAAAGCCTCGGCATATATATCAGGTCTCCGTGGGCTCAAGAACTTCCCTCGGCCTATATGGATGACTCTACGTTCAGAAGAAACAGGAGGAGGTGGGATGAGAAGTGAGGCCTTTGTCTCTGTAGTGAAACACATGAGGTCTTTTGTTCCAGCCGTGTATGTTGTTACAATCGCCTTCCCTGAAGCTCGATCGGATTCACCTCAAACACTGTTACAACTCTTCTACGAGAGGCTGAGGCGACGAAAGTCATATGTGGTGTATGTGCATGTTAAGCGGCATGAGATTAGGGGTTTGCCTCGTAAACATGGTGCCCTTGAGAAATGGTGTGAGGATGTGTTGGAGGCCAAGCAAGCCTTATTGGACCAACACAGAGCAGACCAAACTTTTGGTGACCAAGTGCAAGTACTAGAAACATGCTCTGGTAAACGCCGGCCACTCAAGCCTCTTTTGGTTGTTATCTCTTGGGCACTTGTATTTGTATTCTCGGCAATCAAGTTCCCCCTCTTATCTTTACTAGTGCTACTCATTGCATCCTCAGGCCTGACTTACTTTCTCAATCGGGGCCGCTTGTCTTCCCTACTGCTGAATCTTACAGCCTTCCTTCTTAGACGTGCTTTTGGTTCTCAATTTACGAACAATATCACGCCACACTCTCGCTTGCACTCAAAGAAGCACGAAACTAGAAGACAACCTACTGCTGTGCGCCTGGGTGTTGTGTTTTGGCTCGTTGATTATACGTGGGTGAGTCCAGAAGGGAAAGAGGTCTGCAACTTTGCCATGGTTGATTAG

Protein Analysis

415

Amino Acids

47.31

Weight (kDa)

9.58

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransf_C PF16076 232 - 308 1.7e-12 Acyltransferase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 767
AciI CCGC 2 cut(s) 733, 1024
AcoI YGGCCR 1 cut(s) 890
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 1 cut(s) 660
AfaI GTAC 2 cut(s) 216, 864
AfeI AGCGCT 1 cut(s) 326
AfiI CCNNNNNNNGG 2 cut(s) 316, 392
AgsI TTSAA 2 cut(s) 83, 212
AhlI ACTAGT 1 cut(s) 973
AjiI CACGTC 1 cut(s) 1070
AjnI CCWGG 1 cut(s) 1164
AluBI AGCT 2 cut(s) 345, 644
AluI AGCT 2 cut(s) 345, 644
Alw26I GTCTC 2 cut(s) 488, 580
Aor51HI AGCGCT 1 cut(s) 326
AoxI GGCC 9 cut(s) 61, 107, 199, 509, 567, 801, 890, 997, 1021
ApeKI GCWGC 1 cut(s) 342
ApoI RAATTY 1 cut(s) 368
ArsI GACNNNNNNTTYG 2 cut(s) 827, 859
AspLEI GCGC 2 cut(s) 327, 1164
AspS9I GGNCC 3 cut(s) 446, 820, 1021
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 5 cut(s) 82, 364, 648, 860, 1213
AvaII GGWCC 2 cut(s) 446, 820
AxyI CCTNAGG 1 cut(s) 994
BaeGI GKGCMC 2 cut(s) 772, 928
BanI GGYRCC 1 cut(s) 767
BanII GRGCYC 1 cut(s) 495
BarI GAAGNNNNNNTAC 2 cut(s) 1135, 1167
BauI CACGAG 1 cut(s) 182
BbsI GAAGAC 2 cut(s) 1023, 1150
BbvCI CCTCAGC 1 cut(s) 692
BbvI GCAGC 1 cut(s) 354
BccI CCATC 1 cut(s) 247
BceAI ACGGC 1 cut(s) 596
BciT130I CCWGG 1 cut(s) 1166
BcnI CCSGG 1 cut(s) 311
BcoDI GTCTC 2 cut(s) 488, 580
BcuI ACTAGT 1 cut(s) 973
BfaI CTAG 3 cut(s) 866, 974, 1140
BfmI CTRYAG 2 cut(s) 340, 579
BfoI RGCGCY 1 cut(s) 328
BisI GCNGC 3 cut(s) 343, 734, 1024
BlpI GCTNAGC 1 cut(s) 321
BlsI GCNGC 3 cut(s) 344, 735, 1025
BmcAI AGTACT 1 cut(s) 864
Bme1390I CCNGG 2 cut(s) 311, 1166
Bme18I GGWCC 2 cut(s) 446, 820
BmgBI CACGTC 1 cut(s) 1070
BmgT120I GGNCC 3 cut(s) 446, 820, 1021
BmiI GGNNCC 3 cut(s) 447, 769, 1022
BmrFI CCNGG 2 cut(s) 311, 1166
BmsI GCATC 2 cut(s) 37, 998
BoxI GACNNNNGTC 1 cut(s) 703
BpiI GAAGAC 2 cut(s) 1023, 1150
BpmI CTGGAG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 692
Bpu1102I GCTNAGC 1 cut(s) 321
BpuEI CTTGAG 4 cut(s) 224, 479, 794, 881
BpuMI CCSGG 1 cut(s) 311
BsaAI YACGTR 1 cut(s) 1197
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 5 cut(s) 465, 487, 506, 1165, 1236
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 392
Bse118I RCCGGY 2 cut(s) 245, 888
Bse21I CCTNAGG 1 cut(s) 994
Bse3DI GCAATG 1 cut(s) 984
BseBI CCWGG 1 cut(s) 1166
BseDI CCNNGG 5 cut(s) 465, 487, 506, 1165, 1236
BseGI GGATG 5 cut(s) 306, 525, 561, 796, 989
BseLI CCNNNNNNNGG 2 cut(s) 316, 392
BseMI GCAATG 1 cut(s) 984
BseMII CTCAG 3 cut(s) 335, 683, 1008
BseRI GAGGAG 1 cut(s) 561
BseSI GKGCMC 2 cut(s) 772, 928
BseXI GCAGC 1 cut(s) 354
Bsh1285I CGRYCG 1 cut(s) 650
BshFI GGCC 9 cut(s) 63, 109, 201, 511, 569, 803, 892, 999, 1023
BshNI GGYRCC 1 cut(s) 767
BsiEI CGRYCG 1 cut(s) 650
BsiSI CCGG 3 cut(s) 246, 311, 889
BslI CCNNNNNNNGG 2 cut(s) 316, 392
BsmAI GTCTC 2 cut(s) 488, 580
BsnI GGCC 9 cut(s) 63, 109, 201, 511, 569, 803, 892, 999, 1023
Bso31I GGTCTC 1 cut(s) 488
Bsp1286I GDGCHC 3 cut(s) 495, 772, 928
Bsp143I GATC 1 cut(s) 647
Bsp1720I GCTNAGC 1 cut(s) 321
Bsp19I CCATGG 1 cut(s) 1236
BspACI CCGC 2 cut(s) 733, 1024
BspANI GGCC 9 cut(s) 63, 109, 201, 511, 569, 803, 892, 999, 1023
BspCNI CTCAG 3 cut(s) 334, 684, 1007
BspLI GGNNCC 3 cut(s) 447, 769, 1022
BspMAI CTGCAG 1 cut(s) 344
BspT107I GGYRCC 1 cut(s) 767
BspTNI GGTCTC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 984
BsrFI RCCGGY 2 cut(s) 245, 888
BssAI RCCGGY 2 cut(s) 245, 888
BssECI CCNNGG 5 cut(s) 465, 487, 506, 1165, 1236
BssMI GATC 1 cut(s) 647
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 1 cut(s) 1236
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 1166
Bst4CI ACNGT 2 cut(s) 25, 669
Bst6I CTCTTC 1 cut(s) 683
BstBAI YACGTR 1 cut(s) 1197
BstC8I GCNNGC 4 cut(s) 199, 810, 890, 1118
BstDEI CTNAG 5 cut(s) 117, 321, 692, 994, 1064
BstDSI CCRYGG 2 cut(s) 487, 1236
BstEII GGTNACC 1 cut(s) 848
BstF5I GGATG 5 cut(s) 306, 525, 561, 796, 989
BstH2I RGCGCY 1 cut(s) 328
BstHHI GCGC 2 cut(s) 327, 1164
BstKTI GATC 1 cut(s) 650
BstMAI GTCTC 2 cut(s) 488, 580
BstMBI GATC 1 cut(s) 647
BstMCI CGRYCG 1 cut(s) 650
BstMWI GCNNNNNNNGC 5 cut(s) 333, 342, 730, 809, 898
BstNI CCWGG 1 cut(s) 1166
BstNSI RCATGY 2 cut(s) 728, 876
BstPAI GACNNNNGTC 1 cut(s) 703
BstPI GGTNACC 1 cut(s) 848
BstSCI CCNGG 2 cut(s) 309, 1164
BstSFI CTRYAG 2 cut(s) 340, 579
BstSLI GKGCMC 2 cut(s) 772, 928
BstV1I GCAGC 1 cut(s) 354
BstV2I GAAGAC 2 cut(s) 1023, 1150
BstXI CCANNNNNNTGG 1 cut(s) 845
Bsu36I CCTNAGG 1 cut(s) 994
BsuRI GGCC 9 cut(s) 63, 109, 201, 511, 569, 803, 892, 999, 1023
BtgI CCRYGG 2 cut(s) 487, 1236
BtgZI GCGATG 1 cut(s) 416
BtrI CACGTC 1 cut(s) 1070
BtsCI GGATG 5 cut(s) 306, 525, 561, 796, 989
BtsIMutI CAGTG 2 cut(s) 30, 665
Cac8I GCNNGC 4 cut(s) 199, 810, 890, 1118
CfoI GCGC 2 cut(s) 327, 1164
Cfr10I RCCGGY 2 cut(s) 245, 888
Cfr13I GGNCC 3 cut(s) 446, 820, 1021
Csp6I GTAC 2 cut(s) 215, 863
CviAII CATG 6 cut(s) 592, 725, 737, 764, 873, 1237
CviQI GTAC 2 cut(s) 215, 863
DdeI CTNAG 5 cut(s) 117, 321, 692, 994, 1064
DpnI GATC 1 cut(s) 649
DpnII GATC 1 cut(s) 647
EaeI YGGCCR 1 cut(s) 890
Eam1104I CTCTTC 1 cut(s) 683
EarI CTCTTC 1 cut(s) 683
Eco130I CCWWGG 1 cut(s) 1236
Eco147I AGGCCT 4 cut(s) 63, 201, 569, 999
Eco24I GRGCYC 1 cut(s) 495
Eco31I GGTCTC 1 cut(s) 488
Eco32I GATATC 1 cut(s) 298
Eco47I GGWCC 2 cut(s) 446, 820
Eco47III AGCGCT 1 cut(s) 326
Eco57I CTGAAG 1 cut(s) 660
Eco81I CCTNAGG 1 cut(s) 994
Eco91I GGTNACC 1 cut(s) 848
EcoO65I GGTNACC 1 cut(s) 848
EcoRII CCWGG 1 cut(s) 1164
EcoRV GATATC 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 1236
EcoT38I GRGCYC 1 cut(s) 495
ErhI CCWWGG 1 cut(s) 1236
FaeI CATG 6 cut(s) 595, 728, 740, 767, 876, 1240
FalI AAGNNNNNCTT 2 cut(s) 797, 829
FatI CATG 6 cut(s) 591, 724, 736, 763, 872, 1236
FauNDI CATATG 1 cut(s) 709
Fnu4HI GCNGC 3 cut(s) 343, 734, 1024
FokI GGATG 5 cut(s) 293, 532, 568, 803, 976
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 3 cut(s) 343, 734, 1024
FspBI CTAG 3 cut(s) 866, 974, 1140
GlaI GCGC 2 cut(s) 326, 1163
GluI GCNGC 3 cut(s) 343, 734, 1024
GsuI CTGGAG 1 cut(s) 188
HaeII RGCGCY 1 cut(s) 328
HaeIII GGCC 9 cut(s) 63, 109, 201, 511, 569, 803, 892, 999, 1023
HapII CCGG 3 cut(s) 246, 311, 889
HhaI GCGC 2 cut(s) 327, 1164
Hin1II CATG 6 cut(s) 595, 728, 740, 767, 876, 1240
Hin6I GCGC 2 cut(s) 325, 1162
HinP1I GCGC 2 cut(s) 325, 1162
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 7 cut(s) 73, 103, 208, 523, 653, 1045, 1204
HpaI GTTAAC 1 cut(s) 361
HpaII CCGG 3 cut(s) 246, 311, 889
HphI GGTGA 5 cut(s) 82, 364, 648, 860, 1213
Hpy166II GTNNAC 4 cut(s) 217, 361, 761, 884
Hpy188I TCNGA 4 cut(s) 415, 450, 535, 652
Hpy188III TCNNGA 3 cut(s) 100, 496, 1208
Hpy8I GTNNAC 4 cut(s) 217, 361, 761, 884
Hpy99I CGWCG 1 cut(s) 702
HpyAV CCTTC 6 cut(s) 126, 392, 643, 1066, 1070, 1205
HpyCH4III ACNGT 2 cut(s) 25, 669
HpyCH4IV ACGT 3 cut(s) 529, 1069, 1196
HpyCH4V TGCA 7 cut(s) 28, 342, 724, 859, 989, 1120, 1227
HpyF10VI GCNNNNNNNGC 5 cut(s) 333, 342, 730, 809, 898
HpyF3I CTNAG 5 cut(s) 117, 321, 692, 994, 1064
HpySE526I ACGT 3 cut(s) 529, 1069, 1196
Hsp92II CATG 6 cut(s) 595, 728, 740, 767, 876, 1240
HspAI GCGC 2 cut(s) 325, 1162
KroI GCCGGC 1 cut(s) 888
KroNI GCCGGC 1 cut(s) 890
KspAI GTTAAC 1 cut(s) 361
Kzo9I GATC 1 cut(s) 647
Lsp1109I GCAGC 1 cut(s) 354
LweI GCATC 2 cut(s) 37, 998
MaeI CTAG 3 cut(s) 866, 974, 1140
MaeII ACGT 3 cut(s) 529, 1069, 1196
MaeIII GTNAC 4 cut(s) 434, 622, 669, 848
MalI GATC 1 cut(s) 649
MboI GATC 1 cut(s) 647
MboII GAAGA 5 cut(s) 85, 548, 670, 1023, 1155
MhlI GDGCHC 3 cut(s) 495, 772, 928
MluCI AATT 5 cut(s) 155, 283, 301, 368, 1085
MlyI GAGTC 2 cut(s) 517, 1213
MmeI TCCRAC 1 cut(s) 777
MroNI GCCGGC 1 cut(s) 888
MseI TTAA 2 cut(s) 360, 729
MspI CCGG 3 cut(s) 246, 311, 889
MspR9I CCNGG 2 cut(s) 311, 1166
MvaI CCWGG 1 cut(s) 1166
MwoI GCNNNNNNNGC 5 cut(s) 333, 342, 730, 809, 898
NaeI GCCGGC 1 cut(s) 890
NciI CCSGG 1 cut(s) 311
NcoI CCATGG 1 cut(s) 1236
NdeI CATATG 1 cut(s) 709
NdeII GATC 1 cut(s) 647
NgoMIV GCCGGC 1 cut(s) 888
NlaIII CATG 6 cut(s) 595, 728, 740, 767, 876, 1240
NlaIV GGNNCC 3 cut(s) 447, 769, 1022
NmeAIII GCCGAG 3 cut(s) 446, 487, 923
NmuCI GTSAC 1 cut(s) 848
NspI RCATGY 2 cut(s) 728, 876
PceI AGGCCT 4 cut(s) 63, 201, 569, 999
PdiI GCCGGC 1 cut(s) 890
PfeI GAWTC 5 cut(s) 73, 103, 208, 653, 1045
PkrI GCNGC 3 cut(s) 344, 735, 1025
Ple19I CGATCG 1 cut(s) 650
PleI GAGTC 2 cut(s) 517, 1212
PpsI GAGTC 2 cut(s) 517, 1212
Ppu21I YACGTR 1 cut(s) 1197
PshAI GACNNNNGTC 1 cut(s) 703
Psp6I CCWGG 1 cut(s) 1164
PspEI GGTNACC 1 cut(s) 848
PspGI CCWGG 1 cut(s) 1164
PspN4I GGNNCC 3 cut(s) 447, 769, 1022
PspPI GGNCC 3 cut(s) 446, 820, 1021
PstI CTGCAG 1 cut(s) 344
PvuI CGATCG 1 cut(s) 650
RsaI GTAC 2 cut(s) 216, 864
RsaNI GTAC 2 cut(s) 215, 863
SaqAI TTAA 2 cut(s) 360, 729
SatI GCNGC 3 cut(s) 343, 734, 1024
Sau3AI GATC 1 cut(s) 647
Sau96I GGNCC 3 cut(s) 446, 820, 1021
ScaI AGTACT 1 cut(s) 864
SchI GAGTC 2 cut(s) 517, 1213
ScrFI CCNGG 2 cut(s) 311, 1166
SduI GDGCHC 3 cut(s) 495, 772, 928
SfaNI GCATC 2 cut(s) 37, 998
SfcI CTRYAG 2 cut(s) 340, 579
SinI GGWCC 2 cut(s) 446, 820
SmlI CTYRAG 4 cut(s) 203, 494, 773, 896
SmoI CTYRAG 4 cut(s) 203, 494, 773, 896
SpeI ACTAGT 1 cut(s) 973
Sse9I AATT 5 cut(s) 155, 283, 301, 368, 1085
SseBI AGGCCT 4 cut(s) 63, 201, 569, 999
SsiI CCGC 2 cut(s) 733, 1024
SspMI CTAG 3 cut(s) 866, 974, 1140
StuI AGGCCT 4 cut(s) 63, 201, 569, 999
StyD4I CCNGG 2 cut(s) 309, 1164
StyI CCWWGG 1 cut(s) 1236
TaaI ACNGT 2 cut(s) 25, 669
TaiI ACGT 3 cut(s) 532, 1072, 1199
TaqI TCGA 1 cut(s) 646
TasI AATT 5 cut(s) 155, 283, 301, 368, 1085
TatI WGTACW 2 cut(s) 214, 862
TauI GCSGC 2 cut(s) 736, 1026
TfiI GAWTC 5 cut(s) 73, 103, 208, 653, 1045
Tru1I TTAA 2 cut(s) 360, 729
Tru9I TTAA 2 cut(s) 360, 729
TscAI CASTG 2 cut(s) 30, 672
TseFI GTSAC 1 cut(s) 848
TseI GCWGC 1 cut(s) 342
Tsp45I GTSAC 1 cut(s) 848
TspDTI ATGAA 2 cut(s) 24, 295
TspGWI ACGGA 3 cut(s) 156, 303, 476
TspRI CASTG 2 cut(s) 30, 672
VpaK11BI GGWCC 2 cut(s) 446, 820
XapI RAATTY 1 cut(s) 368
XceI RCATGY 2 cut(s) 728, 876
XspI CTAG 3 cut(s) 866, 974, 1140
ZrmI AGTACT 1 cut(s) 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.