RLG00000018845

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019069
Physical Location & Seq
Reverse (-)
37670259 .. 37672644
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018845

Sequence Viewer

Length: 681 bp
ATGGGGACCGATCTCGTGTACAAAGTCAGACCAAAAATCCAAAACCCGGTCGCCGTTTGCAGTTTAAAACCATCCCTGAAGCCGTCGCATCTTCCATTAAAAGAGAAGTCACAGCAAATCCCACCACCAATCCAGGCCCTAACCCCGATCCTCACAACCTGGGAAACATCAAACAACATCAATCCAAATCTGGATCGATTACCCAACCTCTGCCACAACCAATTCAGACCAAGATCACAACAAATACAAAAAGACAAATCTGGATTGTCAAATCCCCGACTGGTGAGGGATCTGAGAATTGAATCAAATGGCGTCGTAGGGGCACGGAGGTCCGATTGGCCAGGCCTGGGAAGCAGGTTGTCTGCAGCCACTGCCGTTGCCGTCAAGGCCCTGCTCGTCGAGCTGCCGTTAGATCGGAAATCGATCTTTGCAAATCGAAACCCCAGAGTTGCTCCGCTTTGGGCCAGAGAACCCTTCCATTGCCTAGATCGATTCATCATCGCTGCGGCACCAGCAGACTCGGTGGTCACTGGCCTTGTAATTGCAAGCCCCGCCGACTTGGCCTTCAAGTTGCTCCACACAATTCCAGCCAGACCTCCATGCCCAGACCTCCATTGCGATGGCTGTACATCACCGGCCTTGAGCCGCGCTCTATTTGGTCGGATTCGACCAAAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

24.79

Weight (kDa)

10.21

Isoelectric Point (pI)

45.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 524
Acc36I ACCTGC 1 cut(s) 345
AccB1I GGYRCC 1 cut(s) 508
AccII CGCG 1 cut(s) 648
AciI CCGC 4 cut(s) 455, 506, 552, 646
AclWI GGATC 3 cut(s) 142, 201, 297
AcoI YGGCCR 1 cut(s) 338
AcuI CTGAAG 1 cut(s) 98
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 2 cut(s) 20, 628
AfiI CCNNNNNNNGG 2 cut(s) 46, 347
AgsI TTSAA 2 cut(s) 302, 568
AjnI CCWGG 4 cut(s) 132, 158, 340, 345
AluBI AGCT 1 cut(s) 403
AluI AGCT 1 cut(s) 403
AlwI GGATC 3 cut(s) 142, 201, 297
AlwNI CAGNNNCTG 1 cut(s) 371
AoxI GGCC 8 cut(s) 135, 338, 343, 387, 462, 532, 561, 636
ApeKI GCWGC 3 cut(s) 365, 403, 503
AspLEI GCGC 1 cut(s) 650
AspS9I GGNCC 5 cut(s) 6, 136, 330, 388, 462
AsuC2I CCSGG 1 cut(s) 47
AsuHPI GGTGA 2 cut(s) 295, 624
AvaII GGWCC 2 cut(s) 6, 330
BaeGI GKGCMC 1 cut(s) 325
BalI TGGCCA 1 cut(s) 340
BanI GGYRCC 1 cut(s) 508
BauI CACGAG 1 cut(s) 14
BbvI GCAGC 3 cut(s) 377, 390, 490
BccI CCATC 2 cut(s) 79, 614
BceAI ACGGC 5 cut(s) 38, 67, 359, 365, 391
BciT130I CCWGG 4 cut(s) 134, 160, 342, 347
BcnI CCSGG 1 cut(s) 47
BfaI CTAG 1 cut(s) 485
BfmI CTRYAG 1 cut(s) 363
BfuAI ACCTGC 1 cut(s) 345
BglI GCCNNNNNGGC 2 cut(s) 386, 560
BisI GCNGC 5 cut(s) 366, 404, 504, 507, 646
BlsI GCNGC 5 cut(s) 367, 405, 505, 508, 647
Bme1390I CCNGG 5 cut(s) 47, 134, 160, 342, 347
Bme18I GGWCC 2 cut(s) 6, 330
BmgT120I GGNCC 5 cut(s) 6, 136, 330, 388, 462
BmiI GGNNCC 2 cut(s) 7, 510
BmrFI CCNGG 5 cut(s) 47, 134, 160, 342, 347
BmsI GCATC 1 cut(s) 97
BplI GAGNNNNNCTC 2 cut(s) 634, 666
BpuEI CTTGAG 1 cut(s) 661
BpuMI CCSGG 1 cut(s) 47
Bsa29I ATCGAT 3 cut(s) 196, 422, 490
BsaHI GRCGYC 1 cut(s) 312
BsaJI CCNNGG 2 cut(s) 159, 346
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 347
Bse118I RCCGGY 1 cut(s) 634
Bse1I ACTGG 2 cut(s) 285, 535
Bse3DI GCAATG 2 cut(s) 478, 613
BseBI CCWGG 4 cut(s) 134, 160, 342, 347
BseCI ATCGAT 3 cut(s) 196, 422, 490
BseDI CCNNGG 2 cut(s) 159, 346
BseGI GGATG 1 cut(s) 71
BseLI CCNNNNNNNGG 2 cut(s) 46, 347
BseMI GCAATG 2 cut(s) 478, 613
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 2 cut(s) 285, 535
BseSI GKGCMC 1 cut(s) 325
BseXI GCAGC 3 cut(s) 377, 390, 490
Bsh1236I CGCG 1 cut(s) 648
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 8 cut(s) 137, 340, 345, 389, 464, 534, 563, 638
BshNI GGYRCC 1 cut(s) 508
BshVI ATCGAT 3 cut(s) 196, 422, 490
BsiEI CGRYCG 1 cut(s) 51
BsiSI CCGG 2 cut(s) 47, 635
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 2 cut(s) 46, 347
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 8 cut(s) 137, 340, 345, 389, 464, 534, 563, 638
Bsp1286I GDGCHC 1 cut(s) 325
Bsp1407I TGTACA 2 cut(s) 18, 626
Bsp143I GATC 8 cut(s) 10, 147, 193, 233, 289, 412, 423, 487
BspACI CCGC 4 cut(s) 455, 506, 552, 646
BspANI GGCC 8 cut(s) 137, 340, 345, 389, 464, 534, 563, 638
BspCNI CTCAG 1 cut(s) 285
BspDI ATCGAT 3 cut(s) 196, 422, 490
BspFNI CGCG 1 cut(s) 648
BspLI GGNNCC 2 cut(s) 7, 510
BspMAI CTGCAG 1 cut(s) 367
BspMI ACCTGC 1 cut(s) 345
BspPI GGATC 3 cut(s) 142, 201, 297
BspT107I GGYRCC 1 cut(s) 508
BsrDI GCAATG 2 cut(s) 478, 613
BsrFI RCCGGY 1 cut(s) 634
BsrGI TGTACA 2 cut(s) 18, 626
BsrI ACTGG 2 cut(s) 285, 535
BssAI RCCGGY 1 cut(s) 634
BssECI CCNNGG 2 cut(s) 159, 346
BssMI GATC 8 cut(s) 10, 147, 193, 233, 289, 412, 423, 487
BssNI GRCGYC 1 cut(s) 312
BssSI CACGAG 1 cut(s) 14
Bst2BI CACGAG 1 cut(s) 14
Bst2UI CCWGG 4 cut(s) 134, 160, 342, 347
BstACI GRCGYC 1 cut(s) 312
BstAPI GCANNNNNTGC 1 cut(s) 371
BstAUI TGTACA 2 cut(s) 18, 626
BstC8I GCNNGC 1 cut(s) 547
BstDEI CTNAG 1 cut(s) 293
BstF5I GGATG 1 cut(s) 71
BstFNI CGCG 1 cut(s) 648
BstHHI GCGC 1 cut(s) 650
BstKTI GATC 8 cut(s) 13, 150, 196, 236, 292, 415, 426, 490
BstMBI GATC 8 cut(s) 10, 147, 193, 233, 289, 412, 423, 487
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 7 cut(s) 351, 371, 386, 400, 512, 551, 560
BstNI CCWGG 4 cut(s) 134, 160, 342, 347
BstSCI CCNGG 5 cut(s) 45, 132, 158, 340, 345
BstSFI CTRYAG 1 cut(s) 363
BstSLI GKGCMC 1 cut(s) 325
BstUI CGCG 1 cut(s) 648
BstV1I GCAGC 3 cut(s) 377, 390, 490
BstX2I RGATCY 1 cut(s) 289
BstXI CCANNNNNNTGG 1 cut(s) 620
BstYI RGATCY 1 cut(s) 289
Bsu15I ATCGAT 3 cut(s) 196, 422, 490
BsuRI GGCC 8 cut(s) 137, 340, 345, 389, 464, 534, 563, 638
BsuTUI ATCGAT 3 cut(s) 196, 422, 490
BtgZI GCGATG 2 cut(s) 484, 633
BtsCI GGATG 1 cut(s) 71
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 2 cut(s) 369, 528
BveI ACCTGC 1 cut(s) 345
Cac8I GCNNGC 1 cut(s) 547
CaiI CAGNNNCTG 1 cut(s) 371
CfoI GCGC 1 cut(s) 650
Cfr10I RCCGGY 1 cut(s) 634
Cfr13I GGNCC 5 cut(s) 6, 136, 330, 388, 462
ClaI ATCGAT 3 cut(s) 196, 422, 490
CseI GACGC 1 cut(s) 301
Csp6I GTAC 2 cut(s) 19, 627
CspCI CAANNNNNGTGG 2 cut(s) 358, 393
CviAII CATG 1 cut(s) 600
CviQI GTAC 2 cut(s) 19, 627
DdeI CTNAG 1 cut(s) 293
DpnI GATC 8 cut(s) 12, 149, 195, 235, 291, 414, 425, 489
DpnII GATC 8 cut(s) 10, 147, 193, 233, 289, 412, 423, 487
DraI TTTAAA 1 cut(s) 66
DrdI GACNNNNNNGTC 1 cut(s) 524
DseDI GACNNNNNNGTC 1 cut(s) 524
EaeI YGGCCR 1 cut(s) 338
Eco147I AGGCCT 1 cut(s) 345
Eco47I GGWCC 2 cut(s) 6, 330
Eco57I CTGAAG 1 cut(s) 98
EcoO109I RGGNCCY 2 cut(s) 136, 388
EcoRII CCWGG 4 cut(s) 132, 158, 340, 345
FaeI CATG 1 cut(s) 603
FaiI YATR 1 cut(s) 601
FaqI GGGAC 1 cut(s) 19
FatI CATG 1 cut(s) 599
FauI CCCGC 1 cut(s) 559
Fnu4HI GCNGC 5 cut(s) 366, 404, 504, 507, 646
FokI GGATG 1 cut(s) 58
Fsp4HI GCNGC 5 cut(s) 366, 404, 504, 507, 646
FspBI CTAG 1 cut(s) 485
GlaI GCGC 1 cut(s) 649
GluI GCNGC 5 cut(s) 366, 404, 504, 507, 646
HaeIII GGCC 8 cut(s) 137, 340, 345, 389, 464, 534, 563, 638
HapII CCGG 2 cut(s) 47, 635
HgaI GACGC 1 cut(s) 301
HhaI GCGC 1 cut(s) 650
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 1 cut(s) 603
Hin6I GCGC 1 cut(s) 648
HinP1I GCGC 1 cut(s) 648
HinfI GANTC 4 cut(s) 302, 492, 518, 664
HpaII CCGG 2 cut(s) 47, 635
HphI GGTGA 2 cut(s) 295, 624
Hpy166II GTNNAC 1 cut(s) 19
Hpy188I TCNGA 6 cut(s) 29, 227, 294, 334, 417, 663
Hpy188III TCNNGA 3 cut(s) 191, 261, 678
Hpy8I GTNNAC 1 cut(s) 19
Hpy99I CGWCG 3 cut(s) 88, 317, 401
HpyAV CCTTC 2 cut(s) 484, 574
HpyCH4V TGCA 4 cut(s) 60, 365, 431, 545
HpyF10VI GCNNNNNNNGC 7 cut(s) 351, 371, 386, 400, 512, 551, 560
HpyF3I CTNAG 1 cut(s) 293
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 1 cut(s) 603
HspAI GCGC 1 cut(s) 648
Kzo9I GATC 8 cut(s) 10, 147, 193, 233, 289, 412, 423, 487
LmnI GCTCC 2 cut(s) 457, 579
Lsp1109I GCAGC 3 cut(s) 377, 390, 490
LweI GCATC 1 cut(s) 97
MaeI CTAG 1 cut(s) 485
MaeIII GTNAC 2 cut(s) 108, 526
MalI GATC 8 cut(s) 12, 149, 195, 235, 291, 414, 425, 489
MboI GATC 8 cut(s) 10, 147, 193, 233, 289, 412, 423, 487
MboII GAAGA 1 cut(s) 83
MflI RGATCY 1 cut(s) 289
MhlI GDGCHC 1 cut(s) 325
MlsI TGGCCA 1 cut(s) 340
MluCI AATT 4 cut(s) 221, 297, 540, 582
MluNI TGGCCA 1 cut(s) 340
MlyI GAGTC 1 cut(s) 512
MmeI TCCRAC 1 cut(s) 641
MnlI CCTC 6 cut(s) 161, 218, 279, 321, 606, 620
Mox20I TGGCCA 1 cut(s) 340
MscI TGGCCA 1 cut(s) 340
MseI TTAA 2 cut(s) 65, 98
MslI CAYNNNNRTG 1 cut(s) 618
Msp20I TGGCCA 1 cut(s) 340
MspI CCGG 2 cut(s) 47, 635
MspR9I CCNGG 5 cut(s) 47, 134, 160, 342, 347
MvaI CCWGG 4 cut(s) 134, 160, 342, 347
MvnI CGCG 1 cut(s) 648
MwoI GCNNNNNNNGC 7 cut(s) 351, 371, 386, 400, 512, 551, 560
NciI CCSGG 1 cut(s) 47
NdeII GATC 8 cut(s) 10, 147, 193, 233, 289, 412, 423, 487
NlaIII CATG 1 cut(s) 603
NlaIV GGNNCC 2 cut(s) 7, 510
NmuCI GTSAC 2 cut(s) 108, 526
PceI AGGCCT 1 cut(s) 345
PfeI GAWTC 3 cut(s) 302, 492, 664
PkrI GCNGC 5 cut(s) 367, 405, 505, 508, 647
PleI GAGTC 1 cut(s) 512
PpsI GAGTC 1 cut(s) 512
Psp6I CCWGG 4 cut(s) 132, 158, 340, 345
PspGI CCWGG 4 cut(s) 132, 158, 340, 345
PspN4I GGNNCC 2 cut(s) 7, 510
PspPI GGNCC 5 cut(s) 6, 136, 330, 388, 462
PstI CTGCAG 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 371
PsuI RGATCY 1 cut(s) 289
RsaI GTAC 2 cut(s) 20, 628
RsaNI GTAC 2 cut(s) 19, 627
RseI CAYNNNNRTG 1 cut(s) 618
SaqAI TTAA 2 cut(s) 65, 98
SatI GCNGC 5 cut(s) 366, 404, 504, 507, 646
Sau3AI GATC 8 cut(s) 10, 147, 193, 233, 289, 412, 423, 487
Sau96I GGNCC 5 cut(s) 6, 136, 330, 388, 462
SchI GAGTC 1 cut(s) 512
ScrFI CCNGG 5 cut(s) 47, 134, 160, 342, 347
SduI GDGCHC 1 cut(s) 325
SetI ASST 7 cut(s) 161, 210, 332, 359, 405, 598, 612
SfaNI GCATC 1 cut(s) 97
SfcI CTRYAG 1 cut(s) 363
SinI GGWCC 2 cut(s) 6, 330
SmiMI CAYNNNNRTG 1 cut(s) 618
SmlI CTYRAG 1 cut(s) 640
SmoI CTYRAG 1 cut(s) 640
Sse9I AATT 4 cut(s) 221, 297, 540, 582
SseBI AGGCCT 1 cut(s) 345
SsiI CCGC 4 cut(s) 455, 506, 552, 646
SspMI CTAG 1 cut(s) 485
StuI AGGCCT 1 cut(s) 345
StyD4I CCNGG 5 cut(s) 45, 132, 158, 340, 345
TaqI TCGA 6 cut(s) 196, 399, 422, 436, 490, 667
TaqII GACCGA 1 cut(s) 23
TasI AATT 4 cut(s) 221, 297, 540, 582
TatI WGTACW 2 cut(s) 18, 626
TauI GCSGC 2 cut(s) 509, 648
TfiI GAWTC 3 cut(s) 302, 492, 664
Tru1I TTAA 2 cut(s) 65, 98
Tru9I TTAA 2 cut(s) 65, 98
TscAI CASTG 2 cut(s) 376, 535
TseFI GTSAC 2 cut(s) 108, 526
TseI GCWGC 3 cut(s) 365, 403, 503
Tsp45I GTSAC 2 cut(s) 108, 526
TspDTI ATGAA 1 cut(s) 484
TspGWI ACGGA 1 cut(s) 340
TspRI CASTG 2 cut(s) 376, 535
VpaK11BI GGWCC 2 cut(s) 6, 330
XspI CTAG 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.