RLG00000029066

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0019069
Physical Location & Seq
Reverse (-)
32239074 .. 32240993
1920 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029066

Sequence Viewer

Length: 855 bp
ATGCCTCTTCTTACACATAACTCGAGACTTGCCCGGGATCAGGATGGTAGGATTCCTCTTCACTATGCCGCAATGAGAGGACATGCTGAGGTCTTAGCTGAGCTGATGCTTGCAAAACCAGAATCGGTCACATTAAGGGTTCGTGGTGCAGGAGATACGGTTTTTCACTTGTGTGTTATATATAACCAATTAGAGTGCTTGAAACTACTGGTGGAAGAAGTAGGAGACAATAATAGAAACTTCTTGAGTGCTAGAGCTGGTTATGATGGTGGTCTGAGCATCCTCGAATTAGCTGTGATGCTACGGCAACTTGAGACCATAAAATACTTGCTTTCGGTACCTGATATACGAGTAGAAGCAAATGATGTACATAGGAAGGTTTTAAACATGACCACCGCCGCTCCCTTCAGACTGTCTGCAACCACCGTCGTTGCCGTCGAGGCCTCACCCTCATATTGGAATTCGATCTTTGTAGATCGAAACTCCAGAGTTGCTATGATGTGGGCCTGGGAACCTTTCCGTTGCCATGATCGATTCATCATCACTGCACTTCCAGCAAACCGGACTACCGCTGTCAGCCACCATACAGGGTCAGTGGCCACTGGCCTTATAATTGCAAGCACCGTCTGCTTGGCCATCAAGTCACTCCACACAATTCCAGCTAGATCACCTTGCCCAGACCTCCATAGCGTTGGTTGCACATCACCGGCCTTGAGCCGTGCTCGATTTGGTCGGATTCGACCAGAATCACGACTAAAAACCTCTCTGGTCAATGTTCTCCAACGTGAGATTCTGACAGTGGCAAAACCATCCTTCTTCCCAGCAGTTGGTTTGGAGCTTATCAAAAGAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

285

Amino Acids

31.44

Weight (kDa)

9.41

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 11 - 78 1.8e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 50 - 120 2.8e-06 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 611
Acc65I GGTACC 1 cut(s) 337
AccB1I GGYRCC 1 cut(s) 337
AccB7I CCANNNNNTGG 1 cut(s) 827
AccBSI CCGCTC 1 cut(s) 401
AciI CCGC 4 cut(s) 69, 396, 399, 570
AclWI GGATC 1 cut(s) 45
AcoI YGGCCR 2 cut(s) 597, 633
AcsI RAATTY 1 cut(s) 460
AcuI CTGAAG 1 cut(s) 391
AfaI GTAC 2 cut(s) 339, 369
AfiI CCNNNNNNNGG 3 cut(s) 40, 456, 827
AgsI TTSAA 1 cut(s) 202
AjnI CCWGG 1 cut(s) 506
AjuI GAANNNNNNNTTGG 2 cut(s) 774, 806
AleI CACNNNNGTG 1 cut(s) 171
AluBI AGCT 6 cut(s) 98, 103, 257, 293, 662, 838
AluI AGCT 6 cut(s) 98, 103, 257, 293, 662, 838
Alw21I GWGCWC 1 cut(s) 724
Alw26I GTCTC 4 cut(s) 19, 219, 308, 843
AlwI GGATC 1 cut(s) 45
Ama87I CYCGRG 2 cut(s) 22, 33
AoxI GGCC 6 cut(s) 441, 504, 597, 604, 633, 708
ApoI RAATTY 1 cut(s) 460
Asp718I GGTACC 1 cut(s) 337
AspS9I GGNCC 1 cut(s) 504
AsuC2I CCSGG 2 cut(s) 34, 35
AsuHPI GGTGA 3 cut(s) 438, 660, 696
AvaI CYCGRG 2 cut(s) 22, 33
BalI TGGCCA 2 cut(s) 599, 635
BanI GGYRCC 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 724
BbvCI CCTCAGC 1 cut(s) 87
BccI CCATC 4 cut(s) 38, 260, 644, 817
BceAI ACGGC 3 cut(s) 320, 419, 702
BciT130I CCWGG 1 cut(s) 508
BcnI CCSGG 2 cut(s) 34, 35
BcoDI GTCTC 4 cut(s) 19, 219, 308, 843
BfaI CTAG 2 cut(s) 252, 663
BglI GCCNNNNNGGC 1 cut(s) 440
BisI GCNGC 2 cut(s) 69, 399
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 2 cut(s) 70, 400
Bme1390I CCNGG 3 cut(s) 34, 35, 508
BmeT110I CYCGRG 2 cut(s) 22, 33
BmgT120I GGNCC 1 cut(s) 504
BmiI GGNNCC 2 cut(s) 339, 513
BmrFI CCNGG 3 cut(s) 34, 35, 508
BmsI GCATC 3 cut(s) 96, 288, 288
BplI GAGNNNNNCTC 2 cut(s) 706, 738
BpmI CTGGAG 1 cut(s) 469
Bpu10I CCTNAGC 1 cut(s) 87
Bpu1102I GCTNAGC 1 cut(s) 99
BpuEI CTTGAG 3 cut(s) 265, 332, 733
BpuMI CCSGG 2 cut(s) 34, 35
Bsa29I ATCGAT 1 cut(s) 532
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 2 cut(s) 33, 507
BsaWI WCCGGW 1 cut(s) 561
BsaXI ACNNNNNCTCC 4 cut(s) 144, 174, 385, 415
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 456, 827
Bse118I RCCGGY 1 cut(s) 706
Bse1I ACTGG 2 cut(s) 213, 607
Bse3DI GCAATG 1 cut(s) 78
BseBI CCWGG 1 cut(s) 508
BseCI ATCGAT 1 cut(s) 532
BseDI CCNNGG 2 cut(s) 33, 507
BseGI GGATG 3 cut(s) 49, 279, 809
BseLI CCNNNNNNNGG 3 cut(s) 40, 456, 827
BseMI GCAATG 1 cut(s) 78
BseMII CTCAG 3 cut(s) 78, 90, 266
BseNI ACTGG 2 cut(s) 213, 607
BseYI CCCAGC 1 cut(s) 820
BsgI GTGCAG 2 cut(s) 168, 531
BshFI GGCC 6 cut(s) 443, 506, 599, 606, 635, 710
BshNI GGYRCC 1 cut(s) 337
BshVI ATCGAT 1 cut(s) 532
BsiHKAI GWGCWC 1 cut(s) 724
BsiHKCI CYCGRG 2 cut(s) 22, 33
BsiSI CCGG 3 cut(s) 34, 562, 707
BslI CCNNNNNNNGG 3 cut(s) 40, 456, 827
BsmAI GTCTC 4 cut(s) 19, 219, 308, 843
BsnI GGCC 6 cut(s) 443, 506, 599, 606, 635, 710
Bso31I GGTCTC 1 cut(s) 308
BsoBI CYCGRG 2 cut(s) 22, 33
Bsp1286I GDGCHC 1 cut(s) 724
Bsp1407I TGTACA 1 cut(s) 367
Bsp143I GATC 5 cut(s) 37, 465, 475, 529, 665
Bsp1720I GCTNAGC 1 cut(s) 99
BspACI CCGC 4 cut(s) 69, 396, 399, 570
BspANI GGCC 6 cut(s) 443, 506, 599, 606, 635, 710
BspCNI CTCAG 3 cut(s) 79, 91, 267
BspDI ATCGAT 1 cut(s) 532
BspLI GGNNCC 2 cut(s) 339, 513
BspPI GGATC 1 cut(s) 45
BspT107I GGYRCC 1 cut(s) 337
BspTNI GGTCTC 1 cut(s) 308
BsrBI CCGCTC 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 706
BsrGI TGTACA 1 cut(s) 367
BsrI ACTGG 2 cut(s) 213, 607
BssAI RCCGGY 1 cut(s) 706
BssECI CCNNGG 2 cut(s) 33, 507
BssMI GATC 5 cut(s) 37, 465, 475, 529, 665
Bst2UI CCWGG 1 cut(s) 508
Bst4CI ACNGT 5 cut(s) 160, 414, 427, 625, 799
Bst6I CTCTTC 2 cut(s) 12, 63
BstAPI GCANNNNNTGC 1 cut(s) 627
BstAUI TGTACA 1 cut(s) 367
BstC8I GCNNGC 2 cut(s) 111, 619
BstDEI CTNAG 4 cut(s) 87, 94, 99, 275
BstF5I GGATG 3 cut(s) 49, 279, 809
BstKTI GATC 5 cut(s) 40, 468, 478, 532, 668
BstMAI GTCTC 4 cut(s) 19, 219, 308, 843
BstMBI GATC 5 cut(s) 37, 465, 475, 529, 665
BstMWI GCNNNNNNNGC 4 cut(s) 440, 554, 627, 696
BstNI CCWGG 1 cut(s) 508
BstNSI RCATGY 1 cut(s) 86
BstSCI CCNGG 3 cut(s) 32, 33, 506
BstXI CCANNNNNNTGG 1 cut(s) 692
Bsu15I ATCGAT 1 cut(s) 532
BsuRI GGCC 6 cut(s) 443, 506, 599, 606, 635, 710
BsuTUI ATCGAT 1 cut(s) 532
BtsCI GGATG 3 cut(s) 49, 279, 809
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 4 cut(s) 543, 600, 600, 804
Cac8I GCNNGC 2 cut(s) 111, 619
Cfr10I RCCGGY 1 cut(s) 706
Cfr13I GGNCC 1 cut(s) 504
Cfr9I CCCGGG 1 cut(s) 33
ClaI ATCGAT 1 cut(s) 532
Csp6I GTAC 2 cut(s) 338, 368
CspCI CAANNNNNGTGG 2 cut(s) 412, 447
CviAII CATG 3 cut(s) 83, 388, 527
CviQI GTAC 2 cut(s) 338, 368
DdeI CTNAG 4 cut(s) 87, 94, 99, 275
DpnI GATC 5 cut(s) 39, 467, 477, 531, 667
DpnII GATC 5 cut(s) 37, 465, 475, 529, 665
DraI TTTAAA 1 cut(s) 384
EaeI YGGCCR 2 cut(s) 597, 633
Eam1104I CTCTTC 2 cut(s) 12, 63
EarI CTCTTC 2 cut(s) 12, 63
Eco147I AGGCCT 1 cut(s) 443
Eco31I GGTCTC 1 cut(s) 308
Eco57I CTGAAG 1 cut(s) 391
Eco88I CYCGRG 2 cut(s) 22, 33
EcoRI GAATTC 1 cut(s) 460
EcoRII CCWGG 1 cut(s) 506
FaeI CATG 3 cut(s) 86, 391, 530
FatI CATG 3 cut(s) 82, 387, 526
Fnu4HI GCNGC 2 cut(s) 69, 399
FokI GGATG 3 cut(s) 56, 266, 796
Fsp4HI GCNGC 2 cut(s) 69, 399
FspBI CTAG 2 cut(s) 252, 663
GluI GCNGC 2 cut(s) 69, 399
GsaI CCCAGC 1 cut(s) 824
GsuI CTGGAG 1 cut(s) 469
HaeIII GGCC 6 cut(s) 443, 506, 599, 606, 635, 710
HapII CCGG 3 cut(s) 34, 562, 707
Hin1II CATG 3 cut(s) 86, 391, 530
HinfI GANTC 6 cut(s) 52, 122, 534, 736, 746, 790
HpaII CCGG 3 cut(s) 34, 562, 707
HphI GGTGA 3 cut(s) 438, 660, 696
Hpy188I TCNGA 4 cut(s) 276, 410, 735, 795
Hpy188III TCNNGA 5 cut(s) 24, 41, 244, 486, 750
Hpy99I CGWCG 2 cut(s) 431, 440
HpyAV CCTTC 3 cut(s) 370, 415, 823
HpyCH4III ACNGT 5 cut(s) 160, 414, 427, 625, 799
HpyCH4IV ACGT 1 cut(s) 784
HpyCH4V TGCA 6 cut(s) 113, 149, 419, 548, 617, 699
HpyF10VI GCNNNNNNNGC 4 cut(s) 440, 554, 627, 696
HpyF3I CTNAG 4 cut(s) 87, 94, 99, 275
HpySE526I ACGT 1 cut(s) 784
Hsp92II CATG 3 cut(s) 86, 391, 530
KpnI GGTACC 1 cut(s) 341
Kzo9I GATC 5 cut(s) 37, 465, 475, 529, 665
LmnI GCTCC 2 cut(s) 406, 835
LweI GCATC 3 cut(s) 96, 288, 288
MaeI CTAG 2 cut(s) 252, 663
MaeII ACGT 1 cut(s) 784
MaeIII GTNAC 2 cut(s) 127, 642
MalI GATC 5 cut(s) 39, 467, 477, 531, 667
MbiI CCGCTC 1 cut(s) 401
MboI GATC 5 cut(s) 37, 465, 475, 529, 665
MboII GAAGA 3 cut(s) 50, 227, 808
MhlI GDGCHC 1 cut(s) 724
MlsI TGGCCA 2 cut(s) 599, 635
MluCI AATT 5 cut(s) 188, 287, 460, 612, 654
MluNI TGGCCA 2 cut(s) 599, 635
MmeI TCCRAC 2 cut(s) 713, 805
Mox20I TGGCCA 2 cut(s) 599, 635
MscI TGGCCA 2 cut(s) 599, 635
MseI TTAA 2 cut(s) 134, 383
MslI CAYNNNNRTG 1 cut(s) 171
Msp20I TGGCCA 2 cut(s) 599, 635
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 3 cut(s) 34, 562, 707
MspR9I CCNGG 3 cut(s) 34, 35, 508
MvaI CCWGG 1 cut(s) 508
MwoI GCNNNNNNNGC 4 cut(s) 440, 554, 627, 696
NciI CCSGG 2 cut(s) 34, 35
NdeII GATC 5 cut(s) 37, 465, 475, 529, 665
NlaIII CATG 3 cut(s) 86, 391, 530
NlaIV GGNNCC 2 cut(s) 339, 513
NmuCI GTSAC 2 cut(s) 127, 642
NspI RCATGY 1 cut(s) 86
OliI CACNNNNGTG 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 22
PceI AGGCCT 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 435
PfeI GAWTC 6 cut(s) 52, 122, 534, 736, 746, 790
PflMI CCANNNNNTGG 1 cut(s) 827
PkrI GCNGC 2 cut(s) 70, 400
PsiI TTATAA 1 cut(s) 611
Psp6I CCWGG 1 cut(s) 506
PspFI CCCAGC 1 cut(s) 820
PspGI CCWGG 1 cut(s) 506
PspN4I GGNNCC 2 cut(s) 339, 513
PspPI GGNCC 1 cut(s) 504
RsaI GTAC 2 cut(s) 339, 369
RsaNI GTAC 2 cut(s) 338, 368
RseI CAYNNNNRTG 1 cut(s) 171
SaqAI TTAA 2 cut(s) 134, 383
SatI GCNGC 2 cut(s) 69, 399
Sau3AI GATC 5 cut(s) 37, 465, 475, 529, 665
Sau96I GGNCC 1 cut(s) 504
ScrFI CCNGG 3 cut(s) 34, 35, 508
SduI GDGCHC 1 cut(s) 724
SfaNI GCATC 3 cut(s) 96, 288, 288
Sfr274I CTCGAG 1 cut(s) 22
SlaI CTCGAG 1 cut(s) 22
SmaI CCCGGG 1 cut(s) 35
SmiMI CAYNNNNRTG 1 cut(s) 171
SmlI CTYRAG 4 cut(s) 22, 244, 311, 712
SmoI CTYRAG 4 cut(s) 22, 244, 311, 712
Sse9I AATT 5 cut(s) 188, 287, 460, 612, 654
SseBI AGGCCT 1 cut(s) 443
SsiI CCGC 4 cut(s) 69, 396, 399, 570
SspMI CTAG 2 cut(s) 252, 663
StuI AGGCCT 1 cut(s) 443
StyD4I CCNGG 3 cut(s) 32, 33, 506
TaaI ACNGT 5 cut(s) 160, 414, 427, 625, 799
TaiI ACGT 1 cut(s) 787
TaqI TCGA 8 cut(s) 23, 285, 438, 464, 478, 532, 724, 739
TaqII GACCGA 1 cut(s) 115
TasI AATT 5 cut(s) 188, 287, 460, 612, 654
TatI WGTACW 1 cut(s) 367
TauI GCSGC 2 cut(s) 71, 401
TfiI GAWTC 6 cut(s) 52, 122, 534, 736, 746, 790
Tru1I TTAA 2 cut(s) 134, 383
Tru9I TTAA 2 cut(s) 134, 383
TscAI CASTG 4 cut(s) 550, 600, 607, 804
TseFI GTSAC 2 cut(s) 127, 642
Tsp45I GTSAC 2 cut(s) 127, 642
TspDTI ATGAA 1 cut(s) 526
TspGWI ACGGA 1 cut(s) 509
TspMI CCCGGG 1 cut(s) 33
TspRI CASTG 4 cut(s) 550, 600, 607, 804
Van91I CCANNNNNTGG 1 cut(s) 827
XapI RAATTY 1 cut(s) 460
XceI RCATGY 1 cut(s) 86
XhoI CTCGAG 1 cut(s) 22
XmaI CCCGGG 1 cut(s) 33
XspI CTAG 2 cut(s) 252, 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.