RLG00000020514

B-box zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015020
Physical Location & Seq
Reverse (-)
65824238 .. 65826124
1887 bp
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UTR
Exon/CDS
Intron
RLM00000020514

Sequence Viewer

Length: 648 bp
ATGAAATTACAGTGCAATGCGTGCGAGGTGGCCGAGGCCAAAGTGCTGTGCTGCGCAGACGAGGCAGCCTTATGCTGGGGATGTGACCAGAAGATTCACACCGCCAACAAGTTAGCCAGTAAGCACCAGAGAGTCCCTCTCTCTTCCTCATCGTCTCTAATGCCCAAGTGCGACATCTGCCAGGAGACTGTTGGTTATTTCTTCTGTCTAGAGGACCGAGCTTTACTCTGTAGGAAATGCGACGTTGCCATACACACAGCAAACTCCTACGTGTCTAGTCACCAAAGGTTTTTGCTTACTGGAGTGGAAGTGGGGCTTGAAGCAACTGAACCATGCATCTCCCCCTCTACAACGGAAAAGTTGAGTTATGTGGAAACAGTTTCTGAATCAGCATCATCTCCATCAATCCCCAGAAGTGTCATAGATCATTTTAGTACTCGTGAGATAAAAGACACAATTCCTATGCAAATTGGTGGCATTGGGACTCTTGTATCAGACAAGATACCGTTAAACCGAAGTACCACTACCTCTGTGGGTACTATTCCGGGGTGGCTCTTGGAAGAATGTCTTGGATTAACTTATCCTCATCAGAATTACGGATGCTTGGACACTATGCCATCAAAGGTTTATGAAAGCTACTACTCGTGA

Protein Analysis

216

Amino Acids

23.54

Weight (kDa)

5.5

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 55 - 95 5.1e-07 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0015020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78600 AT1G78600
fragaria_vesca FvH4_6g37790
malus_domestica MD09G1155900.v1.1
prunus_persica Prupe.8G109100_v2.0.a1
pyrus_communis pycom09g07470
rosa_chinensis RchiOBHm_Chr2g0150861
rosa_laevigata RLG00000020514
rosa_multiflora Rmu_sc0000185.1_g000012 Rmu_sc0007617.1_g000006
rosa_rugosa Rorug02G0423000
rosa_samantha Rh2AG483300 Rh2BG495700 Rh2CG469600 Rh2DG506600
rosa_wichuraiana Rw2G039610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 55
AciI CCGC 1 cut(s) 102
AcoI YGGCCR 1 cut(s) 30
AfaI GTAC 3 cut(s) 436, 520, 538
AfiI CCNNNNNNNGG 1 cut(s) 75
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 1 cut(s) 320
AjnI CCWGG 1 cut(s) 180
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AluBI AGCT 2 cut(s) 221, 636
AluI AGCT 2 cut(s) 221, 636
Alw26I GTCTC 2 cut(s) 159, 179
AlwNI CAGNNNCTG 1 cut(s) 383
AoxI GGCC 2 cut(s) 30, 36
ApeKI GCWGC 2 cut(s) 51, 65
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 214
AsuC2I CCSGG 1 cut(s) 546
AsuHPI GGTGA 1 cut(s) 272
AvaII GGWCC 1 cut(s) 214
BaeI ACNNNNGTAYC 4 cut(s) 474, 502, 507, 535
BarI GAAGNNNNNNTAC 2 cut(s) 508, 540
BauI CACGAG 2 cut(s) 438, 643
BbvI GCAGC 2 cut(s) 38, 77
BccI CCATC 2 cut(s) 409, 625
BciT130I CCWGG 1 cut(s) 182
BcnI CCSGG 1 cut(s) 546
BcoDI GTCTC 2 cut(s) 159, 179
BfaI CTAG 2 cut(s) 209, 276
BfmI CTRYAG 1 cut(s) 229
BisI GCNGC 2 cut(s) 52, 66
BlsI GCNGC 2 cut(s) 53, 67
BmcAI AGTACT 1 cut(s) 436
Bme1390I CCNGG 2 cut(s) 182, 546
Bme18I GGWCC 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 214
BmrFI CCNGG 2 cut(s) 182, 546
BmsI GCATC 3 cut(s) 345, 401, 590
BplI GAGNNNNNCTC 6 cut(s) 121, 153, 123, 155, 210, 242
BpmI CTGGAG 1 cut(s) 321
BpuMI CCSGG 1 cut(s) 546
BsaAI YACGTR 1 cut(s) 271
BsaJI CCNNGG 2 cut(s) 33, 545
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 1 cut(s) 75
Bse1I ACTGG 2 cut(s) 117, 304
Bse3DI GCAATG 1 cut(s) 22
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 2 cut(s) 33, 545
BseGI GGATG 2 cut(s) 86, 605
BseLI CCNNNNNNNGG 1 cut(s) 75
BseMI GCAATG 1 cut(s) 22
BseNI ACTGG 2 cut(s) 117, 304
BseXI GCAGC 2 cut(s) 38, 77
BseYI CCCAGC 1 cut(s) 75
BshFI GGCC 2 cut(s) 32, 38
BsiSI CCGG 1 cut(s) 545
BslFI GGGAC 2 cut(s) 119, 496
BslI CCNNNNNNNGG 1 cut(s) 75
BsmAI GTCTC 2 cut(s) 159, 179
BsmBI CGTCTC 1 cut(s) 159
BsmFI GGGAC 2 cut(s) 119, 496
BsnI GGCC 2 cut(s) 32, 38
Bsp143I GATC 1 cut(s) 424
BspACI CCGC 1 cut(s) 102
BspANI GGCC 2 cut(s) 32, 38
BsrDI GCAATG 1 cut(s) 22
BsrI ACTGG 2 cut(s) 117, 304
BssECI CCNNGG 2 cut(s) 33, 545
BssMI GATC 1 cut(s) 424
BssSI CACGAG 2 cut(s) 438, 643
Bst2BI CACGAG 2 cut(s) 438, 643
Bst2UI CCWGG 1 cut(s) 182
Bst4CI ACNGT 4 cut(s) 12, 190, 379, 507
Bst6I CTCTTC 1 cut(s) 148
BstAPI GCANNNNNTGC 1 cut(s) 21
BstBAI YACGTR 1 cut(s) 271
BstC8I GCNNGC 1 cut(s) 22
BstF5I GGATG 2 cut(s) 86, 605
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 1 cut(s) 427
BstMAI GTCTC 2 cut(s) 159, 179
BstMBI GATC 1 cut(s) 424
BstMWI GCNNNNNNNGC 3 cut(s) 21, 62, 177
BstNI CCWGG 1 cut(s) 182
BstSCI CCNGG 2 cut(s) 180, 544
BstSFI CTRYAG 1 cut(s) 229
BstV1I GCAGC 2 cut(s) 38, 77
BsuRI GGCC 2 cut(s) 32, 38
BtsCI GGATG 2 cut(s) 86, 605
BtsIMutI CAGTG 1 cut(s) 17
Cac8I GCNNGC 1 cut(s) 22
CaiI CAGNNNCTG 1 cut(s) 383
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 214
Csp6I GTAC 3 cut(s) 435, 519, 537
CviAII CATG 1 cut(s) 333
CviJI RGCY 8 cut(s) 32, 38, 68, 116, 221, 316, 553, 636
CviKI_1 RGCY 8 cut(s) 32, 38, 68, 116, 221, 316, 553, 636
CviQI GTAC 3 cut(s) 435, 519, 537
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 180
EcoT22I ATGCAT 1 cut(s) 338
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 1 cut(s) 336
FaiI YATR 8 cut(s) 73, 251, 334, 369, 422, 464, 614, 630
FalI AAGNNNNNCTT 4 cut(s) 300, 332, 552, 584
FaqI GGGAC 2 cut(s) 119, 496
FatI CATG 1 cut(s) 332
Fnu4HI GCNGC 2 cut(s) 52, 66
FokI GGATG 2 cut(s) 93, 612
Fsp4HI GCNGC 2 cut(s) 52, 66
FspBI CTAG 2 cut(s) 209, 276
FspI TGCGCA 1 cut(s) 55
GlaI GCGC 1 cut(s) 55
GluI GCNGC 2 cut(s) 52, 66
GsaI CCCAGC 1 cut(s) 79
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 2 cut(s) 32, 38
HapII CCGG 1 cut(s) 545
HhaI GCGC 1 cut(s) 56
Hin1II CATG 1 cut(s) 336
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HinfI GANTC 4 cut(s) 94, 132, 386, 484
HpaII CCGG 1 cut(s) 545
HphI GGTGA 1 cut(s) 272
Hpy188I TCNGA 3 cut(s) 385, 496, 591
Hpy188III TCNNGA 3 cut(s) 209, 440, 645
Hpy99I CGWCG 1 cut(s) 245
HpyCH4III ACNGT 4 cut(s) 12, 190, 379, 507
HpyCH4IV ACGT 2 cut(s) 243, 270
HpyCH4V TGCA 3 cut(s) 15, 336, 466
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 62, 177
HpySE526I ACGT 2 cut(s) 243, 270
Hsp92II CATG 1 cut(s) 336
HspAI GCGC 1 cut(s) 54
Kzo9I GATC 1 cut(s) 424
LpnPI CCDG 9 cut(s) 61, 101, 130, 140, 167, 194, 285, 424, 558
Lsp1109I GCAGC 2 cut(s) 38, 77
LweI GCATC 3 cut(s) 345, 401, 590
MaeI CTAG 2 cut(s) 209, 276
MaeII ACGT 2 cut(s) 243, 270
MaeIII GTNAC 2 cut(s) 83, 278
MalI GATC 1 cut(s) 426
MboI GATC 1 cut(s) 424
MboII GAAGA 4 cut(s) 103, 135, 193, 572
MluCI AATT 4 cut(s) 5, 456, 468, 592
MlyI GAGTC 2 cut(s) 141, 478
MnlI CCTC 9 cut(s) 19, 28, 55, 147, 157, 205, 355, 538, 594
Mph1103I ATGCAT 1 cut(s) 338
MseI TTAA 2 cut(s) 509, 575
MspI CCGG 1 cut(s) 545
MspR9I CCNGG 2 cut(s) 182, 546
MvaI CCWGG 1 cut(s) 182
MwoI GCNNNNNNNGC 3 cut(s) 21, 62, 177
NciI CCSGG 1 cut(s) 546
NdeII GATC 1 cut(s) 424
NlaIII CATG 1 cut(s) 336
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 2 cut(s) 83, 278
NsbI TGCGCA 1 cut(s) 55
NsiI ATGCAT 1 cut(s) 338
PfeI GAWTC 2 cut(s) 94, 386
PkrI GCNGC 2 cut(s) 53, 67
PleI GAGTC 2 cut(s) 140, 478
PpsI GAGTC 2 cut(s) 140, 478
Ppu21I YACGTR 1 cut(s) 271
Psp6I CCWGG 1 cut(s) 180
PspFI CCCAGC 1 cut(s) 75
PspGI CCWGG 1 cut(s) 180
PspPI GGNCC 1 cut(s) 214
PstNI CAGNNNCTG 1 cut(s) 383
RsaI GTAC 3 cut(s) 436, 520, 538
RsaNI GTAC 3 cut(s) 435, 519, 537
SaqAI TTAA 2 cut(s) 509, 575
SatI GCNGC 2 cut(s) 52, 66
Sau3AI GATC 1 cut(s) 424
Sau96I GGNCC 1 cut(s) 214
ScaI AGTACT 1 cut(s) 436
SchI GAGTC 2 cut(s) 141, 478
ScrFI CCNGG 2 cut(s) 182, 546
SetI ASST 8 cut(s) 30, 223, 246, 273, 290, 530, 627, 638
SfaNI GCATC 3 cut(s) 345, 401, 590
SfcI CTRYAG 1 cut(s) 229
SinI GGWCC 1 cut(s) 214
Sse9I AATT 4 cut(s) 5, 456, 468, 592
SsiI CCGC 1 cut(s) 102
SspMI CTAG 2 cut(s) 209, 276
StyD4I CCNGG 2 cut(s) 180, 544
TaaI ACNGT 4 cut(s) 12, 190, 379, 507
TaiI ACGT 2 cut(s) 246, 273
TaqII GACCGA 1 cut(s) 231
TasI AATT 4 cut(s) 5, 456, 468, 592
TatI WGTACW 1 cut(s) 434
TfiI GAWTC 2 cut(s) 94, 386
Tru1I TTAA 2 cut(s) 509, 575
Tru9I TTAA 2 cut(s) 509, 575
TscAI CASTG 1 cut(s) 17
TseFI GTSAC 2 cut(s) 83, 278
TseI GCWGC 2 cut(s) 51, 65
Tsp45I GTSAC 2 cut(s) 83, 278
TspDTI ATGAA 2 cut(s) 17, 645
TspGWI ACGGA 2 cut(s) 368, 612
TspRI CASTG 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 214
XbaI TCTAGA 1 cut(s) 208
XcmI CCANNNNNNNNNTGG 2 cut(s) 188, 529
XspI CTAG 2 cut(s) 209, 276
ZrmI AGTACT 1 cut(s) 436
Zsp2I ATGCAT 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.