Rw2G039610

B-box zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015020
Physical Location & Seq
Reverse (-)
65043348 .. 65045798
2451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G039610.1

Sequence Viewer

Length: 663 bp
ATGAAAATACGGTGCAATGCGTGCGAGGTGGCAGAGGCCGAAGTGCTGTGCTGCGCAGACGAGGCAGCCTTATGCTGGGGATGTGACCAGAAGATCCACGCCGCCAACAAGTTAGCCAGTAAGCACCAGAGAGTCCCTCTCTCTTCCTCATCGTCTCCATTGCCCAAGTGCGACTTCTGCCAGGAGACTGTTGGTTACTTCTTCTGTCTAGAGGACCGAGCTTTACTCTGTAGGAAATGCGACGTTGCCATACACACAGCAAACTCCTACGTGTCTAGTCACCAAAGGTTTTTGCTTACTGGAGTGGAAGTGGGGCTTGAAGCAACTGAACCATGTAACTCCCCCTCTACAAAGGAAAAGTTGAGTTCTGTGGAAACAGTTTCTGAATCAGCATCATCTCCATCAATCCCCAGAAGTGTCATAGATCATTTTAGTACTGGTGAGATAAAAGACACAATTCCTATGCAAATTGGTGGCATTGGGACTCTTGTATCAGACAAGATACCGTTAAACCGAAGTACCACTACCTCTGTGAGTACTATTTCGGGGTGGCTCTTGGAAGAATGTCTTGGATTAACTTATCCTAATCAGAATAACGGAAGCTTGGACACTATGCCATCAAAGTGGTATCGATCACTATGTAACCCAGATAGCAAAAAGTAA

Protein Analysis

220

Amino Acids

23.91

Weight (kDa)

5.75

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 54 - 95 7.6e-07 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78600 AT1G78600
fragaria_vesca FvH4_6g37790
malus_domestica MD09G1155900.v1.1
prunus_persica Prupe.8G109100_v2.0.a1
pyrus_communis pycom09g07470
rosa_chinensis RchiOBHm_Chr2g0150861
rosa_laevigata RLG00000020514
rosa_multiflora Rmu_sc0000185.1_g000012 Rmu_sc0007617.1_g000006
rosa_rugosa Rorug02G0423000
rosa_samantha Rh2AG483300 Rh2BG495700 Rh2CG469600 Rh2DG506600
rosa_wichuraiana Rw2G039610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 55
AciI CCGC 1 cut(s) 102
AclWI GGATC 1 cut(s) 88
AfaI GTAC 3 cut(s) 436, 520, 538
AfiI CCNNNNNNNGG 1 cut(s) 75
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 1 cut(s) 320
AjnI CCWGG 1 cut(s) 180
AjuI GAANNNNNNNTTGG 4 cut(s) 158, 190, 552, 584
AluBI AGCT 2 cut(s) 221, 603
AluI AGCT 2 cut(s) 221, 603
Alw26I GTCTC 2 cut(s) 159, 179
AlwI GGATC 1 cut(s) 88
AlwNI CAGNNNCTG 1 cut(s) 383
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 2 cut(s) 51, 65
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 214
AsuHPI GGTGA 2 cut(s) 272, 452
AvaII GGWCC 1 cut(s) 214
BaeI ACNNNNGTAYC 4 cut(s) 474, 502, 507, 535
BarI GAAGNNNNNNTAC 2 cut(s) 508, 540
BbvI GCAGC 2 cut(s) 38, 77
BccI CCATC 2 cut(s) 409, 625
BciT130I CCWGG 1 cut(s) 182
BcoDI GTCTC 2 cut(s) 159, 179
BfaI CTAG 2 cut(s) 209, 276
BfmI CTRYAG 1 cut(s) 229
BisI GCNGC 3 cut(s) 52, 66, 102
BlsI GCNGC 3 cut(s) 53, 67, 103
BmcAI AGTACT 2 cut(s) 436, 538
Bme1390I CCNGG 1 cut(s) 182
Bme18I GGWCC 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 214
BmrFI CCNGG 1 cut(s) 182
BmsI GCATC 1 cut(s) 401
BplI GAGNNNNNCTC 6 cut(s) 121, 153, 123, 155, 210, 242
BpmI CTGGAG 1 cut(s) 321
Bsa29I ATCGAT 1 cut(s) 631
BsaAI YACGTR 1 cut(s) 271
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 1 cut(s) 75
Bse1I ACTGG 3 cut(s) 117, 304, 442
Bse3DI GCAATG 2 cut(s) 22, 158
BseBI CCWGG 1 cut(s) 182
BseCI ATCGAT 1 cut(s) 631
BseGI GGATG 1 cut(s) 86
BseLI CCNNNNNNNGG 1 cut(s) 75
BseMI GCAATG 2 cut(s) 22, 158
BseNI ACTGG 3 cut(s) 117, 304, 442
BseXI GCAGC 2 cut(s) 38, 77
BseYI CCCAGC 1 cut(s) 75
BshFI GGCC 1 cut(s) 38
BshVI ATCGAT 1 cut(s) 631
BslFI GGGAC 2 cut(s) 119, 496
BslI CCNNNNNNNGG 1 cut(s) 75
BsmAI GTCTC 2 cut(s) 159, 179
BsmBI CGTCTC 1 cut(s) 159
BsmFI GGGAC 2 cut(s) 119, 496
BsnI GGCC 1 cut(s) 38
Bsp143I GATC 3 cut(s) 93, 424, 632
BspACI CCGC 1 cut(s) 102
BspANI GGCC 1 cut(s) 38
BspDI ATCGAT 1 cut(s) 631
BspPI GGATC 1 cut(s) 88
BsrDI GCAATG 2 cut(s) 22, 158
BsrI ACTGG 3 cut(s) 117, 304, 442
BssMI GATC 3 cut(s) 93, 424, 632
Bst2UI CCWGG 1 cut(s) 182
Bst4CI ACNGT 4 cut(s) 12, 190, 379, 507
Bst6I CTCTTC 1 cut(s) 148
BstAPI GCANNNNNTGC 1 cut(s) 21
BstBAI YACGTR 1 cut(s) 271
BstC8I GCNNGC 1 cut(s) 22
BstF5I GGATG 1 cut(s) 86
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 3 cut(s) 96, 427, 635
BstMAI GTCTC 2 cut(s) 159, 179
BstMBI GATC 3 cut(s) 93, 424, 632
BstMWI GCNNNNNNNGC 3 cut(s) 21, 62, 177
BstNI CCWGG 1 cut(s) 182
BstSCI CCNGG 1 cut(s) 180
BstSFI CTRYAG 1 cut(s) 229
BstV1I GCAGC 2 cut(s) 38, 77
BstX2I RGATCY 1 cut(s) 93
BstXI CCANNNNNNTGG 1 cut(s) 624
BstYI RGATCY 1 cut(s) 93
Bsu15I ATCGAT 1 cut(s) 631
BsuRI GGCC 1 cut(s) 38
BsuTUI ATCGAT 1 cut(s) 631
BtsCI GGATG 1 cut(s) 86
Cac8I GCNNGC 1 cut(s) 22
CaiI CAGNNNCTG 1 cut(s) 383
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 214
ClaI ATCGAT 1 cut(s) 631
Csp6I GTAC 3 cut(s) 435, 519, 537
CviAII CATG 1 cut(s) 333
CviJI RGCY 7 cut(s) 38, 68, 116, 221, 316, 553, 603
CviKI_1 RGCY 7 cut(s) 38, 68, 116, 221, 316, 553, 603
CviQI GTAC 3 cut(s) 435, 519, 537
DpnI GATC 3 cut(s) 95, 426, 634
DpnII GATC 3 cut(s) 93, 424, 632
Eam1104I CTCTTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 180
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 1 cut(s) 336
FaiI YATR 7 cut(s) 73, 251, 334, 422, 464, 614, 640
FalI AAGNNNNNCTT 6 cut(s) 158, 190, 300, 332, 552, 584
FaqI GGGAC 2 cut(s) 119, 496
FatI CATG 1 cut(s) 332
Fnu4HI GCNGC 3 cut(s) 52, 66, 102
FokI GGATG 1 cut(s) 93
Fsp4HI GCNGC 3 cut(s) 52, 66, 102
FspBI CTAG 2 cut(s) 209, 276
FspI TGCGCA 1 cut(s) 55
GlaI GCGC 1 cut(s) 55
GluI GCNGC 3 cut(s) 52, 66, 102
GsaI CCCAGC 1 cut(s) 79
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 1 cut(s) 38
HhaI GCGC 1 cut(s) 56
Hin1II CATG 1 cut(s) 336
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HindIII AAGCTT 1 cut(s) 601
HinfI GANTC 3 cut(s) 132, 386, 484
HphI GGTGA 2 cut(s) 272, 452
Hpy188I TCNGA 3 cut(s) 385, 496, 591
Hpy188III TCNNGA 1 cut(s) 209
Hpy99I CGWCG 1 cut(s) 245
HpyCH4III ACNGT 4 cut(s) 12, 190, 379, 507
HpyCH4IV ACGT 2 cut(s) 243, 270
HpyCH4V TGCA 2 cut(s) 15, 466
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 62, 177
HpySE526I ACGT 2 cut(s) 243, 270
Hsp92II CATG 1 cut(s) 336
HspAI GCGC 1 cut(s) 54
Kzo9I GATC 3 cut(s) 93, 424, 632
LpnPI CCDG 9 cut(s) 61, 101, 130, 140, 167, 194, 285, 423, 424
Lsp1109I GCAGC 2 cut(s) 38, 77
LweI GCATC 1 cut(s) 401
MaeI CTAG 2 cut(s) 209, 276
MaeII ACGT 2 cut(s) 243, 270
MaeIII GTNAC 5 cut(s) 83, 194, 278, 335, 641
MalI GATC 3 cut(s) 95, 426, 634
MboI GATC 3 cut(s) 93, 424, 632
MboII GAAGA 4 cut(s) 103, 135, 193, 572
MflI RGATCY 1 cut(s) 93
MluCI AATT 2 cut(s) 456, 468
MlyI GAGTC 2 cut(s) 141, 478
MnlI CCTC 8 cut(s) 19, 28, 55, 147, 157, 205, 355, 538
MseI TTAA 2 cut(s) 509, 575
MslI CAYNNNNRTG 1 cut(s) 622
MspR9I CCNGG 1 cut(s) 182
MvaI CCWGG 1 cut(s) 182
MwoI GCNNNNNNNGC 3 cut(s) 21, 62, 177
NdeII GATC 3 cut(s) 93, 424, 632
NlaIII CATG 1 cut(s) 336
NmuCI GTSAC 2 cut(s) 83, 278
NsbI TGCGCA 1 cut(s) 55
PfeI GAWTC 1 cut(s) 386
PkrI GCNGC 3 cut(s) 53, 67, 103
PleI GAGTC 2 cut(s) 140, 478
PpsI GAGTC 2 cut(s) 140, 478
Ppu21I YACGTR 1 cut(s) 271
Psp6I CCWGG 1 cut(s) 180
PspFI CCCAGC 1 cut(s) 75
PspGI CCWGG 1 cut(s) 180
PspPI GGNCC 1 cut(s) 214
PstNI CAGNNNCTG 1 cut(s) 383
PsuI RGATCY 1 cut(s) 93
RsaI GTAC 3 cut(s) 436, 520, 538
RsaNI GTAC 3 cut(s) 435, 519, 537
RseI CAYNNNNRTG 1 cut(s) 622
SaqAI TTAA 2 cut(s) 509, 575
SatI GCNGC 3 cut(s) 52, 66, 102
Sau3AI GATC 3 cut(s) 93, 424, 632
Sau96I GGNCC 1 cut(s) 214
ScaI AGTACT 2 cut(s) 436, 538
SchI GAGTC 2 cut(s) 141, 478
ScrFI CCNGG 1 cut(s) 182
SetI ASST 7 cut(s) 30, 223, 246, 273, 290, 530, 605
SfaNI GCATC 1 cut(s) 401
SfcI CTRYAG 1 cut(s) 229
SinI GGWCC 1 cut(s) 214
SmiMI CAYNNNNRTG 1 cut(s) 622
Sse9I AATT 2 cut(s) 456, 468
SsiI CCGC 1 cut(s) 102
SspMI CTAG 2 cut(s) 209, 276
StyD4I CCNGG 1 cut(s) 180
TaaI ACNGT 4 cut(s) 12, 190, 379, 507
TaiI ACGT 2 cut(s) 246, 273
TaqI TCGA 1 cut(s) 631
TaqII GACCGA 1 cut(s) 231
TasI AATT 2 cut(s) 456, 468
TatI WGTACW 2 cut(s) 434, 536
TauI GCSGC 1 cut(s) 104
TfiI GAWTC 1 cut(s) 386
Tru1I TTAA 2 cut(s) 509, 575
Tru9I TTAA 2 cut(s) 509, 575
TseFI GTSAC 2 cut(s) 83, 278
TseI GCWGC 2 cut(s) 51, 65
Tsp45I GTSAC 2 cut(s) 83, 278
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 612
VpaK11BI GGWCC 1 cut(s) 214
XbaI TCTAGA 1 cut(s) 208
XcmI CCANNNNNNNNNTGG 1 cut(s) 188
XspI CTAG 2 cut(s) 209, 276
ZrmI AGTACT 2 cut(s) 436, 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.