RLG00000022689

DNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017511
Physical Location & Seq
Forward (+)
9582924 .. 9583704
781 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022689

Sequence Viewer

Length: 678 bp
ATGACGGCTCTAGATTCTACGGACCTCTTCTTGATTGCCCTAACCGCCTTTTCCCACCCACCCCGATCCTTTGAACTCTCTCTCCCTCCAATTCCCTCCACATTCCTCCACTCTAAACTCATCCCTAGAACCCATTTCGTCGTCGATGCATTCCACTATGCCGGCGATTATTCCACCTCTTACTTCCTCTCTCACTTCCACTCTGATCACTACGGCAGTCTCTCCCCCAATTGGTCCCAAGGCCTCGTCTTTTGCTCCCCCACCACTACTCGTCTTCTCATAGACGTCCTTAACGTCTCTTCACTTTTCGTGGTTCCTCTGCTGCTCAGACAGCCCCTTCTCATTGATGGATGCGAGGTCATGCTTATTGACTCCAATCATTGCCCAGGCGCTGTTCGGAGACTTCCAGAGTGTAATACCAAATTCGTGTTTTCTTCAGAAGAAGAATCTGTTGATTATATTCCTAGCTTGATAGAAAGAGTCAGAGGTGAACATAAGGTTTCTGTGAGAAGTGTTTTGTTCCTTGTTGCTACATATGTGATTGGGAAAGAGAAGATTTTGATAGAAATTGCTCATAGATGTAAGTGTAAAGTACATGTGGATGCTCGAAAAATGGGGATGCTGCATGTTTTGGGTGACAGAGACAGTGAGGTGTTTACGGAGGATAAATGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000012 GO:0000726 GO:0002237 GO:0003006 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009314 GO:0009409 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010154 GO:0010225 GO:0010243 GO:0015074 GO:0016070 GO:0016874 GO:0016886 GO:0022414 GO:0022616 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0046677 GO:0048316 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051103 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0090351 GO:0140097 GO:1901360 GO:1901576 GO:1901652 GO:1901698 GO:1901700 GO:1904975 GO:2000683 GO:2000685
KEGG Pathways
Metabolic & Signaling
ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430
Pfam Domains
Protein Families
DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DRMBL

Protein Analysis

226

Amino Acids

25.38

Weight (kDa)

6.16

Isoelectric Point (pI)

48.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017511)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17360
rosa_chinensis RchiOBHm_Chr3g0493941
rosa_laevigata RLG00000022689
rosa_multiflora Rmu_co8383177.1_g000001 Rmu_sc0000568.1_g000023 Rmu_sc0010399.1_g000002
rosa_samantha Rh3AG313100 Rh3BG348300 Rh3BG349300 Rh3CG346100 Rh3DG348600
rosa_wichuraiana Rw3G027410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 288
AciI CCGC 1 cut(s) 45
AclWI GGATC 1 cut(s) 60
AcsI RAATTY 1 cut(s) 422
AcuI CTGAAG 1 cut(s) 420
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 1 cut(s) 594
AfiI CCNNNNNNNGG 1 cut(s) 231
AflIII ACRYGT 1 cut(s) 595
AgsI TTSAA 1 cut(s) 74
AjnI CCWGG 1 cut(s) 385
AloI GAACNNNNNNTCC 2 cut(s) 66, 98
AluBI AGCT 1 cut(s) 468
AluI AGCT 1 cut(s) 468
Alw26I GTCTC 4 cut(s) 224, 301, 394, 636
AlwI GGATC 1 cut(s) 60
AlwNI CAGNNNCTG 1 cut(s) 392
AoxI GGCC 1 cut(s) 241
ApeKI GCWGC 2 cut(s) 322, 622
ApoI RAATTY 1 cut(s) 422
AspLEI GCGC 1 cut(s) 392
AspS9I GGNCC 2 cut(s) 22, 234
AsuHPI GGTGA 2 cut(s) 500, 647
AvaII GGWCC 2 cut(s) 22, 234
BbsI GAAGAC 1 cut(s) 266
BbvI GCAGC 2 cut(s) 309, 609
BccI CCATC 1 cut(s) 341
BceAI ACGGC 2 cut(s) 21, 229
BciT130I CCWGG 1 cut(s) 387
BclI TGATCA 1 cut(s) 205
BcoDI GTCTC 4 cut(s) 224, 301, 394, 636
BfaI CTAG 3 cut(s) 11, 126, 465
BfoI RGCGCY 1 cut(s) 393
BisI GCNGC 2 cut(s) 323, 623
BlsI GCNGC 2 cut(s) 324, 624
Bme1390I CCNGG 1 cut(s) 387
Bme18I GGWCC 2 cut(s) 22, 234
BmgT120I GGNCC 2 cut(s) 22, 234
BmiI GGNNCC 2 cut(s) 236, 315
BmrFI CCNGG 1 cut(s) 387
BmsI GCATC 4 cut(s) 136, 341, 592, 609
BpiI GAAGAC 1 cut(s) 266
BsaHI GRCGYC 1 cut(s) 285
BsaJI CCNNGG 2 cut(s) 238, 385
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse118I RCCGGY 1 cut(s) 161
Bse3DI GCAATG 1 cut(s) 379
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 2 cut(s) 238, 385
BseGI GGATG 4 cut(s) 120, 356, 607, 624
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMI GCAATG 1 cut(s) 379
BseMII CTCAG 1 cut(s) 340
BseXI GCAGC 2 cut(s) 309, 609
BshFI GGCC 1 cut(s) 243
BsiSI CCGG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 220
BslI CCNNNNNNNGG 1 cut(s) 231
BsmAI GTCTC 4 cut(s) 224, 301, 394, 636
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 220
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 1 cut(s) 243
Bsp143I GATC 2 cut(s) 65, 205
BspACI CCGC 1 cut(s) 45
BspANI GGCC 1 cut(s) 243
BspCNI CTCAG 1 cut(s) 339
BspLI GGNNCC 2 cut(s) 236, 315
BspPI GGATC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 379
BsrFI RCCGGY 1 cut(s) 161
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 2 cut(s) 238, 385
BssMI GATC 2 cut(s) 65, 205
BssNI GRCGYC 1 cut(s) 285
BssT1I CCWWGG 1 cut(s) 238
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 647
Bst6I CTCTTC 2 cut(s) 32, 304
BstACI GRCGYC 1 cut(s) 285
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 1 cut(s) 326
BstF5I GGATG 4 cut(s) 120, 356, 607, 624
BstH2I RGCGCY 1 cut(s) 393
BstHHI GCGC 1 cut(s) 392
BstKTI GATC 2 cut(s) 68, 208
BstMAI GTCTC 4 cut(s) 224, 301, 394, 636
BstMBI GATC 2 cut(s) 65, 205
BstMWI GCNNNNNNNGC 2 cut(s) 44, 331
BstNI CCWGG 1 cut(s) 387
BstNSI RCATGY 2 cut(s) 599, 629
BstSCI CCNGG 1 cut(s) 385
BstV1I GCAGC 2 cut(s) 309, 609
BstV2I GAAGAC 1 cut(s) 266
BsuRI GGCC 1 cut(s) 243
BtsCI GGATG 4 cut(s) 120, 356, 607, 624
BtsIMutI CAGTG 1 cut(s) 652
Cac8I GCNNGC 1 cut(s) 163
CaiI CAGNNNCTG 1 cut(s) 392
CfoI GCGC 1 cut(s) 392
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 2 cut(s) 22, 234
Csp6I GTAC 1 cut(s) 593
CviAII CATG 3 cut(s) 361, 596, 626
CviJI RGCY 4 cut(s) 8, 243, 334, 468
CviKI_1 RGCY 4 cut(s) 8, 243, 334, 468
CviQI GTAC 1 cut(s) 593
DdeI CTNAG 1 cut(s) 326
DpnI GATC 2 cut(s) 67, 207
DpnII GATC 2 cut(s) 65, 205
Eam1104I CTCTTC 2 cut(s) 32, 304
EarI CTCTTC 2 cut(s) 32, 304
Eco130I CCWWGG 1 cut(s) 238
Eco147I AGGCCT 1 cut(s) 243
Eco47I GGWCC 2 cut(s) 22, 234
Eco57I CTGAAG 1 cut(s) 420
EcoRII CCWGG 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 238
EcoT22I ATGCAT 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 238
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 3 cut(s) 364, 599, 629
FaqI GGGAC 1 cut(s) 220
FatI CATG 3 cut(s) 360, 595, 625
FauNDI CATATG 1 cut(s) 535
FbaI TGATCA 1 cut(s) 205
Fnu4HI GCNGC 2 cut(s) 323, 623
FokI GGATG 4 cut(s) 107, 363, 614, 631
Fsp4HI GCNGC 2 cut(s) 323, 623
FspBI CTAG 3 cut(s) 11, 126, 465
GlaI GCGC 1 cut(s) 391
GluI GCNGC 2 cut(s) 323, 623
HaeII RGCGCY 1 cut(s) 393
HaeIII GGCC 1 cut(s) 243
HapII CCGG 1 cut(s) 162
HhaI GCGC 1 cut(s) 392
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 3 cut(s) 364, 599, 629
Hin6I GCGC 1 cut(s) 390
HinP1I GCGC 1 cut(s) 390
HinfI GANTC 4 cut(s) 14, 371, 446, 480
HpaII CCGG 1 cut(s) 162
HphI GGTGA 2 cut(s) 500, 647
Hpy166II GTNNAC 2 cut(s) 491, 657
Hpy188I TCNGA 5 cut(s) 205, 329, 399, 439, 485
Hpy188III TCNNGA 3 cut(s) 11, 31, 407
Hpy8I GTNNAC 2 cut(s) 491, 657
Hpy99I CGWCG 2 cut(s) 143, 146
HpyAV CCTTC 1 cut(s) 347
HpyCH4III ACNGT 1 cut(s) 647
HpyCH4IV ACGT 2 cut(s) 285, 294
HpyCH4V TGCA 2 cut(s) 149, 625
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 331
HpyF3I CTNAG 1 cut(s) 326
HpySE526I ACGT 2 cut(s) 285, 294
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 3 cut(s) 364, 599, 629
HspAI GCGC 1 cut(s) 390
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 2 cut(s) 65, 205
LmnI GCTCC 1 cut(s) 260
LpnPI CCDG 4 cut(s) 175, 372, 399, 420
Lsp1109I GCAGC 2 cut(s) 309, 609
LweI GCATC 4 cut(s) 136, 341, 592, 609
MaeI CTAG 3 cut(s) 11, 126, 465
MaeII ACGT 2 cut(s) 285, 294
MaeIII GTNAC 1 cut(s) 635
MalI GATC 2 cut(s) 67, 207
MboI GATC 2 cut(s) 65, 205
MboII GAAGA 7 cut(s) 19, 266, 291, 426, 452, 455, 565
MfeI CAATTG 1 cut(s) 229
MluCI AATT 4 cut(s) 90, 229, 422, 567
MlyI GAGTC 2 cut(s) 365, 489
Mph1103I ATGCAT 1 cut(s) 151
MroNI GCCGGC 1 cut(s) 161
MseI TTAA 1 cut(s) 291
MslI CAYNNNNRTG 1 cut(s) 600
MspI CCGG 1 cut(s) 162
MspR9I CCNGG 1 cut(s) 387
MunI CAATTG 1 cut(s) 229
Mva1269I GAATGC 1 cut(s) 149
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 2 cut(s) 44, 331
NaeI GCCGGC 1 cut(s) 163
NdeI CATATG 1 cut(s) 535
NdeII GATC 2 cut(s) 65, 205
NgoMIV GCCGGC 1 cut(s) 161
NlaIII CATG 3 cut(s) 364, 599, 629
NlaIV GGNNCC 2 cut(s) 236, 315
NmuCI GTSAC 1 cut(s) 635
NsiI ATGCAT 1 cut(s) 151
NspI RCATGY 2 cut(s) 599, 629
PceI AGGCCT 1 cut(s) 243
PciI ACATGT 1 cut(s) 595
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PctI GAATGC 1 cut(s) 149
PdiI GCCGGC 1 cut(s) 163
PfeI GAWTC 2 cut(s) 14, 446
PkrI GCNGC 2 cut(s) 324, 624
PleI GAGTC 2 cut(s) 365, 488
PpsI GAGTC 2 cut(s) 365, 488
PscI ACATGT 1 cut(s) 595
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 2 cut(s) 236, 315
PspPI GGNCC 2 cut(s) 22, 234
PstNI CAGNNNCTG 1 cut(s) 392
RsaI GTAC 1 cut(s) 594
RsaNI GTAC 1 cut(s) 593
RseI CAYNNNNRTG 1 cut(s) 600
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 2 cut(s) 323, 623
Sau3AI GATC 2 cut(s) 65, 205
Sau96I GGNCC 2 cut(s) 22, 234
SchI GAGTC 2 cut(s) 365, 489
ScrFI CCNGG 1 cut(s) 387
SetI ASST 9 cut(s) 27, 179, 288, 297, 360, 470, 490, 501, 654
SfaNI GCATC 4 cut(s) 136, 341, 592, 609
SinI GGWCC 2 cut(s) 22, 234
SmiMI CAYNNNNRTG 1 cut(s) 600
Sse9I AATT 4 cut(s) 90, 229, 422, 567
SseBI AGGCCT 1 cut(s) 243
SsiI CCGC 1 cut(s) 45
SspMI CTAG 3 cut(s) 11, 126, 465
StuI AGGCCT 1 cut(s) 243
StyD4I CCNGG 1 cut(s) 385
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 1 cut(s) 647
TaiI ACGT 2 cut(s) 288, 297
TaqI TCGA 2 cut(s) 144, 607
TasI AATT 4 cut(s) 90, 229, 422, 567
TatI WGTACW 1 cut(s) 592
TfiI GAWTC 2 cut(s) 14, 446
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 1 cut(s) 652
TseFI GTSAC 1 cut(s) 635
TseI GCWGC 2 cut(s) 322, 622
Tsp45I GTSAC 1 cut(s) 635
TspGWI ACGGA 2 cut(s) 35, 674
TspRI CASTG 1 cut(s) 652
VpaK11BI GGWCC 2 cut(s) 22, 234
XapI RAATTY 1 cut(s) 422
XbaI TCTAGA 1 cut(s) 10
XceI RCATGY 2 cut(s) 599, 629
XspI CTAG 3 cut(s) 11, 126, 465
ZraI GACGTC 1 cut(s) 286
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.