RH3BG348300

DNA ligase

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0017511
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG348300.1

Sequence Viewer

Length: 4194 bp
ATGACGGCTCTGGATTCTACGGACCTCTTCCTGACTGCCCTAACCGCCTTTTCCCACCCACCCAGACCTCTTGAACTCTCTCTCCCTCCAATTCCCTCCACATTCCCCCACTCCAAACTCATCCCCAGAACCCGTTTCGCCGTCGACGCATTCCGCTATGCCGGCGATTATTCCGTCTCTTACTTCCTCTCTCACTTCCACTCCGATCACTACGGCGGTCTCTCCCCCAATTGGTCCCAAGGCCTCGTCTTTTGCTCCCCCACCACCGCTCGTCTTCTCACAGACGTCCTCAACGTCTCTCCGCTTTTCGTGGTTCCTCTGCCGCTCGGACAGCCCCTTCTCATTGATGGATGCGAGGTCGTGCTTATTGACGCCAATCACTGCCCAGGCGCCGTTCAGTTCTTGTTCAGAGTTCCGGCCTCCAATGGAGACTTGCAGAGGTATGTTCACACCGGTGATTTTCGCTTTTCTGCTCCGATGAAGCTAGACCCTTTTCTCTGTAAATTTGTGGGTTCAGACGCCGTTTTCTTGGACACAACTTATTGCAATCCCAAATTCGTGTTTCCTTCCCAAGAAGAATCTGTTGATTATATTTCTAGCTTGATAGAAAGAGTTGGAGTTGGAGGTGAACATGAAGTTTCTGTGAAAAGTGTTCTGTTCCTTGTTGCTACATATGTGATTGGGAAAGAGAAGATTTTGATAGAAATTGCTCGTAGATGTAAGCGTAAAGTGTACGTGGATGCTCCAAAAATGGCGGTGCTGCGTGTTTTGGGTTACGGAGACAGTGGAGTGTTTACGGAGGATGAATGTGAGAGTGATGTGCATGTTGTTGGGTGGAATGTGTTGGGTGAGACTTGGCCATATTTCAGGCCAAATTTTGTGAAAATGGAGGAGATTATGGTTGAGAGGGGGTATTCCAGGGTTGTAGGGTTCGTCCCTACTGGCTGGACTTATGAAGTCAAGCGTAACAAGTTCTCGGTGAGGTCAAAGGATGCTTTTGAGATCCATCTTGTACCCTACAGTGAACATTCAAGCTATGATGAACTTAGAGAATACGTCAGGTTTTTGAAACCAAAGCGTGTGATTCCTACAGTGGGCTCCGATGTGGAAAAGATAGACAGTAAACATGCCAGTAAAATGCAAAAGCATTTTGCTGGGTTAATTGATGAAATGGCCAACAAAAAAGAGTTCTTGAGGGGTTTTCATCGTGGGACTACAGAAGGAGGTGGCAAGGTTCACAGTGATGCCAAAGATTACACGATGGAGGGGCAGGATCAAGAGAAAAAGGCAACACCTTCAGATATTGATACCATGGGAGATACTAATGTAGGCTTTGGTAAGGAATTGTACTCTCCTCTCCAAGAACCTGATTCACAAACTCCAATATTGTTAACTGATGAAAAAGCAGAGGAAATTATTCAACAACTTCGTGATTGTTTGCCGTCATGGGTCACTCGCCAGCAGATGCTAGATCTGATTGGGAGCTCAGGAGGGGACATTGTTGAAGCAGTCACTAAATTTTACGATCGTGAAACAGAATTTCATGATCAAGGAATTGCCTCTACAAGTGCCGTATCTGTATCCGAGACCAGCTCACTCTGTGACACAGCATCACCAACAAAAGCAGGTTCTGTTCATGCAAATATTGATGTTTCTTCAAGCCAAGCTCATATTTCACCCAACCCAAGAAATATTGTTAAAAGTGGCATTTCCCCTAGAAAAAGAGGGAGGAAGATCAGTAATAAAGTTAACAAGAAAGTAAAGTTACAGTCAGAATTGAATTCGTGTGGGCCAAAACAATCGACAATAACAAGGTTTTTTAGTAAAGTTTTGCCTGACGTTACAGAAAGTGCAGAGATTGGATCAGTAGGTGAACAAAACCCCAAAGATAAAAAGTTACCAGATCATGTCACCCAATCATACAAGGATGCAATAGATCAATTTCTTCAGATAATAGATGGCAATGAATCATTAAAAAGCTATGCTGGTACCATTCTTCAAAAGGCAAATGGGGATATTAGTATGGCTGTGGATATACATTACCGTAATAATGAAGGCAGGTCTGATGAGAATGAAGTGGAGCTGGTGGTTGATGCAAAATCAGTTCAGTCCAACTGTGGTATAGATAACTGTTCTTCTGTCAAAAAAAAAATTGAATTAGGGAAAATAGGAGTGGTAGCTGATTTGTCTGTACAGGGGTCATTACCAGATAACGTCGCTGCAACTTCTGTGTCACTACCACCAGAAAAATTCAATCCTGTAGATCATGCATGCTGGAGCAATGGACAACATGCTCCATATTTACATCTTGCACGAACCTTTGACTTACTAGAGGATGAAAAAGGCAAGATAAAGGCTACTTCTATGCTCTGCAATATGTTCAGAAGTTTGCTTGCCCTGTCTCCTGATGATGTGCTGCCTTCAGTTTATTTGTGCACCAATAAGATAGCTGCAGACCATGAAAATGTGGAACTGAACATTGGTGGAAGTTTGGTCACAGCAGCACTGGAGGACGCATGTGGGACAAGCAGATCCAAAATCAGGGATATGTACAATGAATTGGGAGATCTTGGTGATGTTGCTCAAGCATGTCGCCAGACACAGACTTTGCTTGCCCCTCCTCCACCACTTCTAATAAAAGATGTATTTTCTGCACTCCGGAAGATAAGTGTACAAACAGGTACTGGAAGCAGTGCTCGAAAGAGAAGCCTGATTGTGAATCTCTTGCGTTCTTGCAGAGAGAAAGAGATGAAGTTCCTTATTAGAACTTTGGTTCGAAACTTACGTATCGGAGCAATGATGAAAACTGTCCTACCTGCTCTGGCTCAAGCTGTTGTTCTGAATTCCTTCCATAGCTGTAATCACGAAGGAACTATAGAAAGTTTGAAGGACAAGCTTCAGCGCCATTCAGCAGCTGTAGTAGAAGCATACAATGTACTGCCCAATTTGGATTTGGTCGTTCCTTCTCTCATGAAAAGAGGCATTGGATTCTCCTCATCAACTTTGTCAATGGTTCCAGGAATACCTATCAAGCCAATGCTTGCAAGAATCACAAATGGTATTCAGCAAGCACTGAAGCTATTTGAAAAGAAAGCTTTTACATGTGAATACAAATATGATGGCCAGCGTGCACAAATTCATAAACTAGTTGATGGGTCCATGCATGTTTTTACAAGAAATGGGGATGAGTCAACTTCGAGATTTCCAGATCTGATTAATATAATCAATCAATCTTGTAAATCCGATGCAGTGACTTTCATACTGGATGCAGAGATTGTTGGAGTTGATAGGAAGAATGGTTGCAAGCTAATGTCCTTCCAAGAATTATCTTCACGTGGGAGAGGGAGCAGAGATGCATCAATAACGTTGGATAGCATAAAGGTCAACATTTGCGTCTTTGTCTTCGACATCATGTTTGCCAATGGACAACAGTTGTTAAGCTTTCCTCTGCGGAGAAGGCGAAAGTATTTGAAAGAGTTGTTCTACGATGAGAAGTTGGGTTACTTTGAATATGCCAAGGAAATGACAGTTGAGGCAGAGGATGCTTGTTTAACCAGTGAAGCGACATTAACCAAAATAAACTCTTTCCTTGAAAATGCATTTGTTTCCTCATGTGAAGGGATTATAGTTAAATCTTTAGATGTTGATGCTGGATATTCTCCATCAAAGCGTACAGATACTTGGTTAAAGGTCAAGCGAGACTACATGGAGGGATTGGGCGATACTCTTGATTTAGTCCCAATTGGAGCTTGGCATGGAAACGGGAGAAAGGCAGGCTGGTACAGTCCATTTCTCATGGCATGTTACAATCCTGAAACTGAAGAATTTCAAAGCGTCTGTCGTGTTATGTCTGGGTTTTCAGATTTATTTTATACAGAGATGAAAAGTTCTTATTCTGGGGACAAGATCTTGGCCAAAAAACCACCTTATTATAGAACAGCTGAGGTGCCTGATAAGTGGTTTTCTCCTGAGCTTGTATGGGAAATAAGAGGTGCAGACTTTACAGTGTCTCCAGTTCACCAGGCTGCTTTAGGTCTAGTTCATCCATCACGTGGCATTTCCATTCGATTCCCAAGATACATTCGCACTGTGACTGATAGAAAACCAGATGAATGTAGCACAGCTTCTGATTTAGCTGAAATGTTTCATTCTCAGACGAGGAAGATGGATGTGGCTTCTGAAGATGGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000012 GO:0000726 GO:0002237 GO:0003006 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009314 GO:0009409 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010154 GO:0010225 GO:0010243 GO:0015074 GO:0016070 GO:0016874 GO:0016886 GO:0022414 GO:0022616 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0046677 GO:0048316 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051103 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0090351 GO:0140097 GO:1901360 GO:1901576 GO:1901652 GO:1901698 GO:1901700 GO:1904975 GO:2000683 GO:2000685
KEGG Pathways
Metabolic & Signaling
ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430
Pfam Domains
Protein Families
DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DRMBL

Protein Analysis

1397

Amino Acids

155.19

Weight (kDa)

6.41

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 263 - 366 3e-21 DNA repair metallo-beta-lactamase
DNA_ligase_A_N PF04675 766 - 942 4.1e-43 DNA ligase N terminus
DNA_ligase_A_M PF01068 1012 - 1232 1.2e-46 ATP dependent DNA ligase domain
DNA_ligase_A_C PF04679 1258 - 1365 8e-17 ATP dependent DNA ligase C terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017511)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17360
rosa_chinensis RchiOBHm_Chr3g0493941
rosa_laevigata RLG00000022689
rosa_multiflora Rmu_co8383177.1_g000001 Rmu_sc0000568.1_g000023 Rmu_sc0010399.1_g000002
rosa_samantha Rh3AG313100 Rh3BG348300 Rh3BG349300 Rh3CG346100 Rh3DG348600
rosa_wichuraiana Rw3G027410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 288
Acc36I ACCTGC 3 cut(s) 1616, 2049, 2824
Acc65I GGTACC 1 cut(s) 1988
AccB1I GGYRCC 3 cut(s) 389, 1988, 3946
AccB7I CCANNNNNTGG 1 cut(s) 4052
AccBSI CCGCTC 2 cut(s) 269, 325
AccI GTMKAC 1 cut(s) 144
AccIII TCCGGA 1 cut(s) 2658
AciI CCGC 8 cut(s) 45, 154, 216, 267, 302, 323, 755, 3451
AclI AACGTT 1 cut(s) 3365
AclWI GGATC 4 cut(s) 997, 1281, 1870, 2526
AcoI YGGCCR 4 cut(s) 857, 1173, 3121, 3912
AcuI CTGAAG 6 cut(s) 1281, 1931, 2406, 2882, 3095, 3840
AcvI CACGTG 2 cut(s) 3335, 4052
AcyI GRCGYC 4 cut(s) 285, 372, 390, 519
AdeI CACNNNGTG 1 cut(s) 1601
AfiI CCNNNNNNNGG 4 cut(s) 231, 1094, 2541, 4052
AflIII ACRYGT 1 cut(s) 3101
AgeI ACCGGT 1 cut(s) 452
AhlI ACTAGT 1 cut(s) 3145
AjnI CCWGG 4 cut(s) 385, 917, 3016, 4020
AjuI GAANNNNNNNTTGG 2 cut(s) 2463, 2495
AleI CACNNNNGTG 1 cut(s) 453
AloI GAACNNNNNNTCC 2 cut(s) 66, 98
Alw21I GWGCWC 4 cut(s) 1487, 2438, 2698, 3133
Alw44I GTGCAC 2 cut(s) 2434, 3129
AlwI GGATC 4 cut(s) 997, 1281, 1870, 2526
AlwNI CAGNNNCTG 4 cut(s) 1631, 2684, 2915, 4127
Aor13HI TCCGGA 1 cut(s) 2658
AoxI GGCC 8 cut(s) 241, 417, 857, 869, 1173, 1790, 3121, 3912
ApaLI GTGCAC 2 cut(s) 2434, 3129
ApeKI GCWGC 8 cut(s) 760, 2219, 2416, 2450, 2501, 2912, 4025, 4188
ArsI GACNNNNNNTTYG 6 cut(s) 2590, 2622, 3398, 3430, 3823, 3855
AseI ATTAAT 1 cut(s) 3216
AsiGI ACCGGT 1 cut(s) 452
Asp700I GAANNNNTTC 4 cut(s) 1416, 2846, 3825, 4143
Asp718I GGTACC 1 cut(s) 1988
AspLEI GCGC 2 cut(s) 392, 2904
AspS9I GGNCC 4 cut(s) 22, 234, 1790, 3156
AsuII TTCGAA 1 cut(s) 2776
AvaII GGWCC 3 cut(s) 22, 234, 3156
BaeGI GKGCMC 2 cut(s) 2438, 3133
BaeI ACNNNNGTAYC 4 cut(s) 1311, 1344, 2770, 2803
BalI TGGCCA 4 cut(s) 859, 1175, 3123, 3914
BanI GGYRCC 3 cut(s) 389, 1988, 3946
BanII GRGCYC 2 cut(s) 1100, 1487
BarI GAAGNNNNNNTAC 4 cut(s) 3485, 3517, 4108, 4140
BbrPI CACGTG 2 cut(s) 3335, 4052
BbsI GAAGAC 2 cut(s) 266, 3394
Bbv12I GWGCWC 4 cut(s) 1487, 2438, 2698, 3133
BbvCI CCTCAGC 1 cut(s) 3942
BbvI GCAGC 7 cut(s) 747, 2206, 2403, 2437, 2513, 2924, 4012
BceAI ACGGC 7 cut(s) 21, 125, 229, 377, 506, 1426, 1556
BciT130I CCWGG 4 cut(s) 387, 919, 3018, 4022
BciVI GTATCC 1 cut(s) 1591
BclI TGATCA 1 cut(s) 1546
BcuI ACTAGT 1 cut(s) 3145
BfaI CTAG 7 cut(s) 485, 597, 1469, 1716, 2330, 3146, 4037
BfmI CTRYAG 7 cut(s) 1018, 1089, 1215, 2259, 2451, 2874, 2916
BfoI RGCGCY 2 cut(s) 393, 2905
BfuAI ACCTGC 3 cut(s) 1616, 2049, 2824
BfuI GTATCC 1 cut(s) 1591
BglII AGATCT 4 cut(s) 1471, 2566, 3208, 3906
BisI GCNGC 9 cut(s) 323, 761, 2220, 2417, 2451, 2502, 2913, 4026, 4189
BlsI GCNGC 9 cut(s) 324, 762, 2221, 2418, 2452, 2503, 2914, 4027, 4190
Bme1390I CCNGG 4 cut(s) 387, 919, 3018, 4022
Bme18I GGWCC 3 cut(s) 22, 234, 3156
BmgT120I GGNCC 4 cut(s) 22, 234, 1790, 3156
BmiI GGNNCC 8 cut(s) 236, 315, 391, 1099, 1990, 3015, 3157, 3948
BmrFI CCNGG 4 cut(s) 387, 919, 3018, 4022
BpiI GAAGAC 2 cut(s) 266, 3394
BplI GAGNNNNNCTC 2 cut(s) 1577, 1609
BpmI CTGGAG 3 cut(s) 2296, 2528, 3996
Bpu10I CCTNAGC 3 cut(s) 1486, 3942, 3969
Bpu14I TTCGAA 1 cut(s) 2776
BpuEI CTTGAG 3 cut(s) 1213, 2568, 2811
BsaAI YACGTR 4 cut(s) 736, 2786, 3335, 4052
BsaHI GRCGYC 4 cut(s) 285, 372, 390, 519
BsaI GGTCTC 2 cut(s) 224, 1580
BsaJI CCNNGG 5 cut(s) 238, 385, 918, 1311, 3516
BsaWI WCCGGW 2 cut(s) 452, 2658
BsaXI ACNNNNNCTCC 6 cut(s) 66, 96, 185, 215, 3994, 4024
Bsc4I CCNNNNNNNGG 4 cut(s) 231, 1094, 2541, 4052
Bse118I RCCGGY 2 cut(s) 161, 452
Bse1I ACTGG 7 cut(s) 946, 1131, 2511, 2689, 3267, 3555, 4013
Bse3DI GCAATG 3 cut(s) 1969, 2287, 2802
BseAI TCCGGA 1 cut(s) 2658
BseBI CCWGG 4 cut(s) 387, 919, 3018, 4022
BseDI CCNNGG 5 cut(s) 238, 385, 918, 1311, 3516
BseLI CCNNNNNNNGG 4 cut(s) 231, 1094, 2541, 4052
BseMI GCAATG 3 cut(s) 1969, 2287, 2802
BseMII CTCAG 4 cut(s) 1500, 3933, 3960, 4166
BseNI ACTGG 7 cut(s) 946, 1131, 2511, 2689, 3267, 3555, 4013
BseRI GAGGAG 4 cut(s) 905, 1344, 2610, 2983
BseSI GKGCMC 2 cut(s) 2438, 3133
BseXI GCAGC 7 cut(s) 747, 2206, 2403, 2437, 2513, 2924, 4012
BseYI CCCAGC 1 cut(s) 1154
BsgI GTGCAG 3 cut(s) 1872, 2637, 4014
Bsh1285I CGRYCG 1 cut(s) 1528
BshFI GGCC 8 cut(s) 243, 419, 859, 871, 1175, 1792, 3123, 3914
BshNI GGYRCC 3 cut(s) 389, 1988, 3946
BshTI ACCGGT 1 cut(s) 452
BsiEI CGRYCG 1 cut(s) 1528
BsiHKAI GWGCWC 4 cut(s) 1487, 2438, 2698, 3133
BsiSI CCGG 4 cut(s) 162, 416, 453, 2659
BslFI GGGAC 7 cut(s) 220, 920, 1225, 1508, 2536, 3722, 3914
BslI CCNNNNNNNGG 4 cut(s) 231, 1094, 2541, 4052
BsmBI CGTCTC 2 cut(s) 181, 301
BsmFI GGGAC 7 cut(s) 220, 920, 1225, 1508, 2536, 3722, 3914
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 8 cut(s) 243, 419, 859, 871, 1175, 1792, 3123, 3914
Bso31I GGTCTC 2 cut(s) 224, 1580
Bsp119I TTCGAA 1 cut(s) 2776
Bsp1286I GDGCHC 5 cut(s) 1100, 1487, 2438, 2698, 3133
Bsp13I TCCGGA 1 cut(s) 2658
Bsp1407I TGTACA 3 cut(s) 2191, 2550, 2671
Bsp19I CCATGG 1 cut(s) 1311
BspACI CCGC 8 cut(s) 45, 154, 216, 267, 302, 323, 755, 3451
BspANI GGCC 8 cut(s) 243, 419, 859, 871, 1175, 1792, 3123, 3914
BspCNI CTCAG 4 cut(s) 1499, 3934, 3961, 4165
BspEI TCCGGA 1 cut(s) 2658
BspHI TCATGA 2 cut(s) 1543, 2970
BspLI GGNNCC 8 cut(s) 236, 315, 391, 1099, 1990, 3015, 3157, 3948
BspMAI CTGCAG 1 cut(s) 2455
BspMI ACCTGC 3 cut(s) 1616, 2049, 2824
BspPI GGATC 4 cut(s) 997, 1281, 1870, 2526
BspT104I TTCGAA 1 cut(s) 2776
BspT107I GGYRCC 3 cut(s) 389, 1988, 3946
BspTNI GGTCTC 2 cut(s) 224, 1580
BsrBI CCGCTC 2 cut(s) 269, 325
BsrDI GCAATG 3 cut(s) 1969, 2287, 2802
BsrFI RCCGGY 2 cut(s) 161, 452
BsrGI TGTACA 3 cut(s) 2191, 2550, 2671
BsrI ACTGG 7 cut(s) 946, 1131, 2511, 2689, 3267, 3555, 4013
BssAI RCCGGY 2 cut(s) 161, 452
BssECI CCNNGG 5 cut(s) 238, 385, 918, 1311, 3516
BssNI GRCGYC 4 cut(s) 285, 372, 390, 519
BssT1I CCWWGG 3 cut(s) 238, 1311, 3516
Bst2UI CCWGG 4 cut(s) 387, 919, 3018, 4022
Bst6I CTCTTC 1 cut(s) 32
BstACI GRCGYC 4 cut(s) 285, 372, 390, 519
BstAPI GCANNNNNTGC 1 cut(s) 3542
BstAUI TGTACA 3 cut(s) 2191, 2550, 2671
BstBAI YACGTR 4 cut(s) 736, 2786, 3335, 4052
BstBI TTCGAA 1 cut(s) 2776
BstDEI CTNAG 5 cut(s) 1046, 1486, 3942, 3969, 4152
BstDSI CCRYGG 1 cut(s) 1311
BstH2I RGCGCY 2 cut(s) 393, 2905
BstHHI GCGC 2 cut(s) 392, 2904
BstMCI CGRYCG 1 cut(s) 1528
BstMWI GCNNNNNNNGC 6 cut(s) 44, 146, 162, 331, 3457, 3542
BstNI CCWGG 4 cut(s) 387, 919, 3018, 4022
BstNSI RCATGY 9 cut(s) 827, 1130, 2274, 2294, 2520, 2592, 3105, 3167, 3804
BstSCI CCNGG 4 cut(s) 385, 917, 3016, 4020
BstSFI CTRYAG 7 cut(s) 1018, 1089, 1215, 2259, 2451, 2874, 2916
BstSLI GKGCMC 2 cut(s) 2438, 3133
BstSNI TACGTA 1 cut(s) 2786
BstV1I GCAGC 7 cut(s) 747, 2206, 2403, 2437, 2513, 2924, 4012
BstV2I GAAGAC 2 cut(s) 266, 3394
BstX2I RGATCY 6 cut(s) 1002, 1471, 2531, 2566, 3208, 3906
BstYI RGATCY 6 cut(s) 1002, 1471, 2531, 2566, 3208, 3906
BsuI GTATCC 1 cut(s) 1591
BsuRI GGCC 8 cut(s) 243, 419, 859, 871, 1175, 1792, 3123, 3914
BtgI CCRYGG 1 cut(s) 1311
BtsI GCAGTG 3 cut(s) 379, 2698, 3255
BveI ACCTGC 3 cut(s) 1616, 2049, 2824
CaiI CAGNNNCTG 4 cut(s) 1631, 2684, 2915, 4127
CciI TCATGA 2 cut(s) 1543, 2970
CfoI GCGC 2 cut(s) 392, 2904
Cfr10I RCCGGY 2 cut(s) 161, 452
Cfr13I GGNCC 4 cut(s) 22, 234, 1790, 3156
CseI GACGC 6 cut(s) 155, 380, 527, 2522, 3382, 3823
CspAI ACCGGT 1 cut(s) 452
DdeI CTNAG 5 cut(s) 1046, 1486, 3942, 3969, 4152
DinI GGCGCC 1 cut(s) 391
DraIII CACNNNGTG 1 cut(s) 1601
EaeI YGGCCR 4 cut(s) 857, 1173, 3121, 3912
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 1485
Eco105I TACGTA 1 cut(s) 2786
Eco130I CCWWGG 3 cut(s) 238, 1311, 3516
Eco147I AGGCCT 1 cut(s) 243
Eco24I GRGCYC 2 cut(s) 1100, 1487
Eco31I GGTCTC 2 cut(s) 224, 1580
Eco47I GGWCC 3 cut(s) 22, 234, 3156
Eco53kI GAGCTC 1 cut(s) 1485
Eco57I CTGAAG 6 cut(s) 1281, 1931, 2406, 2882, 3095, 3840
Eco72I CACGTG 2 cut(s) 3335, 4052
EcoICRI GAGCTC 1 cut(s) 1485
EcoRI GAATTC 2 cut(s) 1780, 2842
EcoRII CCWGG 4 cut(s) 385, 917, 3016, 4020
EcoT14I CCWWGG 3 cut(s) 238, 1311, 3516
EcoT22I ATGCAT 4 cut(s) 2272, 3165, 3358, 3601
EcoT38I GRGCYC 2 cut(s) 1100, 1487
EgeI GGCGCC 1 cut(s) 391
EheI GGCGCC 1 cut(s) 391
ErhI CCWWGG 3 cut(s) 238, 1311, 3516
Esp3I CGTCTC 2 cut(s) 181, 301
FaqI GGGAC 7 cut(s) 220, 920, 1225, 1508, 2536, 3722, 3914
FauNDI CATATG 1 cut(s) 673
FbaI TGATCA 1 cut(s) 1546
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 9 cut(s) 323, 761, 2220, 2417, 2451, 2502, 2913, 4026, 4189
FriOI GRGCYC 2 cut(s) 1100, 1487
Fsp4HI GCNGC 9 cut(s) 323, 761, 2220, 2417, 2451, 2502, 2913, 4026, 4189
FspBI CTAG 7 cut(s) 485, 597, 1469, 1716, 2330, 3146, 4037
GlaI GCGC 2 cut(s) 391, 2903
GluI GCNGC 9 cut(s) 323, 761, 2220, 2417, 2451, 2502, 2913, 4026, 4189
GsaI CCCAGC 1 cut(s) 1158
GsuI CTGGAG 3 cut(s) 2296, 2528, 3996
HaeII RGCGCY 2 cut(s) 393, 2905
HaeIII GGCC 8 cut(s) 243, 419, 859, 871, 1175, 1792, 3123, 3914
HapII CCGG 4 cut(s) 162, 416, 453, 2659
HgaI GACGC 6 cut(s) 155, 380, 527, 2522, 3382, 3823
HhaI GCGC 2 cut(s) 392, 2904
Hin1I GRCGYC 4 cut(s) 285, 372, 390, 519
Hin6I GCGC 2 cut(s) 390, 2902
HinP1I GCGC 2 cut(s) 390, 2902
HincII GTYRAC 5 cut(s) 145, 1392, 1750, 3192, 3385
HindII GTYRAC 5 cut(s) 145, 1392, 1750, 3192, 3385
HindIII AAGCTT 3 cut(s) 2894, 3093, 3439
HpaI GTTAAC 2 cut(s) 1392, 1750
HpaII CCGG 4 cut(s) 162, 416, 453, 2659
Hpy99I CGWCG 3 cut(s) 146, 149, 2219
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 146, 162, 331, 3457, 3542
HpyF3I CTNAG 5 cut(s) 1046, 1486, 3942, 3969, 4152
Hsp92I GRCGYC 4 cut(s) 285, 372, 390, 519
HspAI GCGC 2 cut(s) 390, 2902
KasI GGCGCC 1 cut(s) 389
Kpn2I TCCGGA 1 cut(s) 2658
KpnI GGTACC 1 cut(s) 1992
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Ksp22I TGATCA 1 cut(s) 1546
KspAI GTTAAC 2 cut(s) 1392, 1750
Lsp1109I GCAGC 7 cut(s) 747, 2206, 2403, 2437, 2513, 2924, 4012
MaeI CTAG 7 cut(s) 485, 597, 1469, 1716, 2330, 3146, 4037
MbiI CCGCTC 2 cut(s) 269, 325
MfeI CAATTG 2 cut(s) 229, 3741
MflI RGATCY 6 cut(s) 1002, 1471, 2531, 2566, 3208, 3906
MhlI GDGCHC 5 cut(s) 1100, 1487, 2438, 2698, 3133
MlsI TGGCCA 4 cut(s) 859, 1175, 3123, 3914
MluNI TGGCCA 4 cut(s) 859, 1175, 3123, 3914
Mly113I GGCGCC 1 cut(s) 390
MlyI GAGTC 1 cut(s) 3197
MmeI TCCRAC 5 cut(s) 595, 601, 2136, 3259, 3348
Mox20I TGGCCA 4 cut(s) 859, 1175, 3123, 3914
Mph1103I ATGCAT 4 cut(s) 2272, 3165, 3358, 3601
MroI TCCGGA 1 cut(s) 2658
MroNI GCCGGC 1 cut(s) 161
MroXI GAANNNNTTC 4 cut(s) 1416, 2846, 3825, 4143
MscI TGGCCA 4 cut(s) 859, 1175, 3123, 3914
MslI CAYNNNNRTG 3 cut(s) 453, 1242, 2463
Msp20I TGGCCA 4 cut(s) 859, 1175, 3123, 3914
MspA1I CMGCKG 3 cut(s) 2915, 3941, 4191
MspI CCGG 4 cut(s) 162, 416, 453, 2659
MspR9I CCNGG 4 cut(s) 387, 919, 3018, 4022
MunI CAATTG 2 cut(s) 229, 3741
Mva1269I GAATGC 1 cut(s) 149
MvaI CCWGG 4 cut(s) 387, 919, 3018, 4022
MwoI GCNNNNNNNGC 6 cut(s) 44, 146, 162, 331, 3457, 3542
NaeI GCCGGC 1 cut(s) 163
NarI GGCGCC 1 cut(s) 390
NcoI CCATGG 1 cut(s) 1311
NdeI CATATG 1 cut(s) 673
NgoMIV GCCGGC 1 cut(s) 161
NlaIV GGNNCC 8 cut(s) 236, 315, 391, 1099, 1990, 3015, 3157, 3948
NmuCI GTSAC 8 cut(s) 1450, 1510, 1601, 1909, 2232, 2494, 3250, 4090
NsiI ATGCAT 4 cut(s) 2272, 3165, 3358, 3601
NspI RCATGY 9 cut(s) 827, 1130, 2274, 2294, 2520, 2592, 3105, 3167, 3804
NspV TTCGAA 1 cut(s) 2776
OliI CACNNNNGTG 1 cut(s) 453
PaeI GCATGC 1 cut(s) 2274
PagI TCATGA 2 cut(s) 1543, 2970
PceI AGGCCT 1 cut(s) 243
PciI ACATGT 1 cut(s) 3101
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 2782
PctI GAATGC 1 cut(s) 149
PdiI GCCGGC 1 cut(s) 163
PdmI GAANNNNTTC 4 cut(s) 1416, 2846, 3825, 4143
PfeI GAWTC 9 cut(s) 14, 578, 1084, 1370, 1967, 2719, 2988, 3048, 4068
PflMI CCANNNNNTGG 1 cut(s) 4052
PfoI TCCNGGA 1 cut(s) 3016
PinAI ACCGGT 1 cut(s) 452
PkrI GCNGC 9 cut(s) 324, 762, 2221, 2418, 2452, 2503, 2914, 4027, 4190
Ple19I CGATCG 1 cut(s) 1528
PleI GAGTC 1 cut(s) 3196
PluTI GGCGCC 1 cut(s) 393
PmaCI CACGTG 2 cut(s) 3335, 4052
PmlI CACGTG 2 cut(s) 3335, 4052
PpsI GAGTC 1 cut(s) 3196
Ppu21I YACGTR 4 cut(s) 736, 2786, 3335, 4052
PscI ACATGT 1 cut(s) 3101
PshBI ATTAAT 1 cut(s) 3216
Psp124BI GAGCTC 1 cut(s) 1487
Psp1406I AACGTT 1 cut(s) 3365
Psp6I CCWGG 4 cut(s) 385, 917, 3016, 4020
PspCI CACGTG 2 cut(s) 3335, 4052
PspFI CCCAGC 1 cut(s) 1154
PspGI CCWGG 4 cut(s) 385, 917, 3016, 4020
PspN4I GGNNCC 8 cut(s) 236, 315, 391, 1099, 1990, 3015, 3157, 3948
PspPI GGNCC 4 cut(s) 22, 234, 1790, 3156
PstI CTGCAG 1 cut(s) 2455
PstNI CAGNNNCTG 4 cut(s) 1631, 2684, 2915, 4127
PsuI RGATCY 6 cut(s) 1002, 1471, 2531, 2566, 3208, 3906
PvuI CGATCG 1 cut(s) 1528
PvuII CAGCTG 3 cut(s) 2915, 3941, 4191
RseI CAYNNNNRTG 3 cut(s) 453, 1242, 2463
SacI GAGCTC 1 cut(s) 1487
SalI GTCGAC 1 cut(s) 143
SatI GCNGC 9 cut(s) 323, 761, 2220, 2417, 2451, 2502, 2913, 4026, 4189
Sau96I GGNCC 4 cut(s) 22, 234, 1790, 3156
SchI GAGTC 1 cut(s) 3197
ScrFI CCNGG 4 cut(s) 387, 919, 3018, 4022
SduI GDGCHC 5 cut(s) 1100, 1487, 2438, 2698, 3133
SfcI CTRYAG 7 cut(s) 1018, 1089, 1215, 2259, 2451, 2874, 2916
SfoI GGCGCC 1 cut(s) 391
SfuI TTCGAA 1 cut(s) 2776
SgrAI CRCCGGYG 1 cut(s) 452
SgrDI CGTCGACG 1 cut(s) 143
SinI GGWCC 3 cut(s) 22, 234, 3156
SmiMI CAYNNNNRTG 3 cut(s) 453, 1242, 2463
SmlI CTYRAG 3 cut(s) 1192, 2583, 2826
SmoI CTYRAG 3 cut(s) 1192, 2583, 2826
SnaBI TACGTA 1 cut(s) 2786
SpeI ACTAGT 1 cut(s) 3145
SphI GCATGC 1 cut(s) 2274
SseBI AGGCCT 1 cut(s) 243
SsiI CCGC 8 cut(s) 45, 154, 216, 267, 302, 323, 755, 3451
SspDI GGCGCC 1 cut(s) 389
SspI AATATT 3 cut(s) 1386, 1645, 1693
SspMI CTAG 7 cut(s) 485, 597, 1469, 1716, 2330, 3146, 4037
SstI GAGCTC 1 cut(s) 1487
StuI AGGCCT 1 cut(s) 243
StyD4I CCNGG 4 cut(s) 385, 917, 3016, 4020
StyI CCWWGG 3 cut(s) 238, 1311, 3516
TaqI TCGA 7 cut(s) 144, 1802, 2698, 2776, 3197, 3405, 4066
TatI WGTACW 5 cut(s) 1347, 2191, 2550, 2671, 2935
TauI GCSGC 1 cut(s) 325
TfiI GAWTC 9 cut(s) 14, 578, 1084, 1370, 1967, 2719, 2988, 3048, 4068
TseFI GTSAC 8 cut(s) 1450, 1510, 1601, 1909, 2232, 2494, 3250, 4090
TseI GCWGC 8 cut(s) 760, 2219, 2416, 2450, 2501, 2912, 4025, 4188
Tsp45I GTSAC 8 cut(s) 1450, 1510, 1601, 1909, 2232, 2494, 3250, 4090
TspGWI ACGGA 4 cut(s) 35, 163, 792, 812
Van91I CCANNNNNTGG 1 cut(s) 4052
VneI GTGCAC 2 cut(s) 2434, 3129
VpaK11BI GGWCC 3 cut(s) 22, 234, 3156
VspI ATTAAT 1 cut(s) 3216
XceI RCATGY 9 cut(s) 827, 1130, 2274, 2294, 2520, 2592, 3105, 3167, 3804
XcmI CCANNNNNNNNNTGG 2 cut(s) 2950, 3747
XmiI GTMKAC 1 cut(s) 144
XmnI GAANNNNTTC 4 cut(s) 1416, 2846, 3825, 4143
XspI CTAG 7 cut(s) 485, 597, 1469, 1716, 2330, 3146, 4037
ZraI GACGTC 1 cut(s) 286
Zsp2I ATGCAT 4 cut(s) 2272, 3165, 3358, 3601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.