Rw3G027410

DNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0017511
Physical Location & Seq
Reverse (-)
38851037 .. 38862461
11425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G027410.1

Sequence Viewer

Length: 4047 bp
ATGACGGCTCTGGATTCTACGGACCTCTTCCTGACTGCCCTAACCGCCTTTTCCCACCCACCCAGACCCAGACCCCTTGAACTCTCTCTCCCTCCAATTCCCTCCACATTCCCCCACTCCAAACTCATCCCCAGAACCCGTTTCGCCGTCGACGCGTTCCGCTATGCCGGCGATTATTCCGTCTCTTACTTCCTCTCTCACTTCCACTCCGATCACTACGGCGGTCTCTCCCCCAATTGGTCCCAAGGCCTCGTCTTTTGCTCCCCCACCACCGCTCGTCTTCTCACAGACGTCCTCAACGTCTCTTCGCTTTTCGTGGTTCCTCTGCCGCTCGGACAGCCCCTTCTCATTGATGGATGCGAGGTCGTGCTTATTGATGCCAATCATTGCCCGGGCGCCGTTCAGTTCTTGTTCAAAGTTCCCGCCTCCAATGGAGACTTGCAGAGGTATGTTCACACCGGTGATTTTCGGTTTTCTGCTCCGATGAAGCTAGACCCTTTTCTCTGTAACTTTGTGGGTTCAGATGCCGTTTTCTTGGATACAACTTATTGCAATCCCAAATTCGTGTTTCCTTCACAAGAAGAATCTGTTGATTATATTTCTAGCTTGATAGAAAGAGAGAAGATTTTGATAGAAATTGCTCGTAGATGTAAGCGTAAAGTGTACGTGGATGCTCGAAAAATGGCGGTGCTGCGTGTTTTGGGTTACGGAGACAGTGGGGTGTTTACGGAGGATGAATGTGAGACTGATGTGCATGTTGTTGGGTGGAATGTGTTGGGTGAGACTTGGCCATATTTCAGGCCAAATTTTGTGAAAATGGAGGAGGTTATGGTTGAGAGGGGGTATTCCAGGGTTGTAGGGTTCGTCCCTACTGGCTGGACTTATGAAGATGCTTTTGAGATCCATCTTGTACCCTACAGTGAACATTCAAACTATGATGAACTTAGAGAATACGTCAGGTTTTTGAAACCAAAGCGTGTGATTCCTACAGTGGGCTCCGATGTGGAAAAGATAGACAGTAAACATGCCAGTAAAATGCAAAAGCATTTTGCGGGGTTAATTGATGAAATGGCCAACAAAAAAGAGTTCTTGAGGGGTTTTCATCATTACACGATGGAGGGGCAGGATCAAGAGAAAAAGGCAACACCTTCAGATATTGATACTATGGGAGATACTAATGTAGGCTTTGGTAAGGAATTGTACTCTCCTCTCCAAGAACCTGATTCACAAACTCCAATATTGTTAACTGATGAAAAAGCAGAGGAAATTATTCAACAACTTCGTGATTGTTTGCCGTCATGGGTCACTCGCCAGCAGATGCTAGATCTGATTGGGAGCTCAGGAGGGGACATTGTTGAAGCAGTCACTAAATTTTACGATCGTGAAACTGAATTTCATGATCAAGGAATTGCCTCTACAAGTGCCGTATCTGTATCTGAGACCAGCTCACTCTGTGACACAGCATCACCGACAAAAGCAGGTTCTGTTCATGCAAGTATTGATGTTTCTTCAAGCCAAGCTCATATTTTACCCAACCCAAGAAATATTGTTAAAAGTGGCATTTCCCCTGGAAAAAGAGGGAGGAAGATCAGTAATAAAGTTAACAAGAAAGTAAAGTTACAGTCAAAACTGAATTCGTGTGGGCCAAAACAATCGACAATAACAAGGTTTTTTAGTAAAGTTTTGCCTGACGTTACAGAAAGTGCAGAGATTGGATCAGTAGGTGAACAAAACCCTAAAGATAAAAAATTACCAGATCATGTCACCCAATCATACAAGGATGCAATAGATCAATTTCTTCAGATAATAGATGGCAATGAATCATTAAAAAGCTATGCTGGTACCATTCTTCAAAAGGCAAATGGGGATATTAGTATGGCTGTGGATATACATTACCGTAATAATGAAGGCAGGTCTGATGAGAATGAAGTGGAGCTGGTGGTTGATGCAAAATCAGTTCAGTCCAACTGTGGTATAGATAACTGTTCTTCTGTCAAAAAAAAAGTTGAATTAGGGAAAATAGGAGTGGTAGCTGATTTGTCTGTACAGGGGTCATTACCAGATAACGTCGCTGCAACTTCTGTGTCACTACCACCAGAAAAATTCAATCCTGTAGATCATGCATGCTGGAGCAATGGACAACATGCTCCATATTTACATCTAGCACGAACCTTTGACTTACTAGAGGATGAAAAAGGCAAGATAAAGGCTACTTCTATGCTCTGCAATATGTTCAGAAGTTTGCTTGCCCTGTCTCCTGATGATGTGCTGCCTTCAGTTTATTTGTGCACCAATAAGATAGCTGCAGACCATGAAAATGTGGAACTGAACATTGGTGGAAGTTTGGTCACAGCAGCACTGGAGGACGCATGTGGGACAAGCAGATCCAAAATCAGGGATATGTACAATGAATTGGGAGATCTTGGTGATGTTGCTCAAGCATGTCGCCAGACACAGACTTTGCTTGCCCCTCCTCCACCACTTCTAATAAAAGATGTATTTTCTGCACTCCGGAAGATAAGTGTACAAACAGGTACTGGAAGCAGTGCTCGAAAGAGAAGCCTGATTGTGAATCTCTTGCGTTCTTGCAGAGAGAAAGAGATGAAGTTCCTTATTAGAACTTTGGTTCGAAACTTACGTATCGGAGCAATGATGAAAACTGTCCTACCTGCTCTGGCTCAAGCTGTTGTTCTGAATTCCTTCCATAGCTGTAATCACGAAGGAACTATAGAAAGTTTGAAGGACAAGCTTCAGCGCCATTCAGCAGCTGTAGTAGAAGCATACAATGTACTGCCCAATTTGGATTTGGTCGTTCCTTCTCTCATGAAAAGAGGCATTGGATTCTCCTCATCAACTTTGTCAATGGTTCCAGGAATACCTATCAAGCCAATGCTTGCAAGAATCACAAATGGTGTTCAGCAAGCACTGAAGCTATTTGAAAAGAAAGCTTTTACATGTGAATACAAATATGATGGCCAGCGTGCACAAATTCATAAACTAGTTGATGGGTCCATGCACGTTTTTACAAGAAATGGGGATGAGTCAACTTCGAGATTTCCAGATCTGATTAATATAATCAATCAATCTTGTAAACCCGATGCAGTGACTTTCATACTGGATGCAGAGATTGTTGGAGTTGATAGGAAGAATGCTTGCAAGCTAATGTCCTTCCAAGAATTATCTTCACGTGGGAGAGGGAGCAGAGATGCATCAATAACGTTGGATAGCATAAAGGTCAACATTTGCGTCTTTGTCTTCGACATCATGTTTGCCAATGGACAACAGTTGTTAAGCTTTCCTCTGCGGAGAAGGCGAAAGTATTTGAAAGAGTTGTTCTACGATGAAAAGTTGGGTTACTTTGAATATGCCAAGGAAATGACAGTTGAGGCAGAGGATGCTTGTTTAACCAGTGAAGCGACATTAACCAAAATAAACTCTTTCCTTGAAAATGCATTTGTTTCCTCATGTGAAGGGATTATAGTTAAATCTTTAGATGTTGATGCTGGATATTCTCCATCAAAGCGTACAGATACTTGGTTAAAGGTCAAGCGAGACTACATGGAGGGATTGGGCGATACTCTTGATTTAGTCCCAATTGGAGCTTGGCATGGAAACGGGAGAAAGGCAGGCTGGTACAGTCCATTTCTCATGGCATGTTACAATCCTGAAACTGAAGAATTTCAAAGCGTCTGTCGTGTTATGTCTGGGTTTTCAGATTTATTTTATACAGAGATGAAAAGTTTTTATTCTGGGGACAAGATCTTGGCCAAAAAACCACCTTATTATAGAACAGCTGAGGAGGCTGATAAGTGGTTTTCTCCTGAGCTCGTATTGGAAATAAGAGGTGCAGACTTTACAGTGTCTCCAGTTCACCAGGCTGCTTTAGGTCTAGTTCATCCATCACGTGGCATTTCCATTCGATTCCCAAGATACATTCGCACTGTGACTGATAGAAAACCAGATGAATGTAGCACAGCTTCTGATTTGGCTGAAATGTTTCATTCTCAGACGAGGAAGATGGATGTGGCTTCTGAAGATGGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000012 GO:0000726 GO:0002237 GO:0003006 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009314 GO:0009409 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010154 GO:0010225 GO:0010243 GO:0015074 GO:0016070 GO:0016874 GO:0016886 GO:0022414 GO:0022616 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0046677 GO:0048316 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051103 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0090351 GO:0140097 GO:1901360 GO:1901576 GO:1901652 GO:1901698 GO:1901700 GO:1904975 GO:2000683 GO:2000685
KEGG Pathways
Metabolic & Signaling
ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430
Pfam Domains
Protein Families
DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DRMBL

Protein Analysis

1348

Amino Acids

150.01

Weight (kDa)

6.19

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 240 - 332 3.8e-16 DNA repair metallo-beta-lactamase
DNA_ligase_A_N PF04675 717 - 893 3.9e-43 DNA ligase N terminus
DNA_ligase_A_M PF01068 963 - 1183 1.4e-46 ATP dependent DNA ligase domain
DNA_ligase_A_C PF04679 1209 - 1316 1.8e-16 ATP dependent DNA ligase C terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017511)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17360
rosa_chinensis RchiOBHm_Chr3g0493941
rosa_laevigata RLG00000022689
rosa_multiflora Rmu_co8383177.1_g000001 Rmu_sc0000568.1_g000023 Rmu_sc0010399.1_g000002
rosa_samantha Rh3AG313100 Rh3BG348300 Rh3BG349300 Rh3CG346100 Rh3DG348600
rosa_wichuraiana Rw3G027410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 294
Acc36I ACCTGC 3 cut(s) 1469, 1902, 2677
Acc65I GGTACC 1 cut(s) 1841
AccB1I GGYRCC 2 cut(s) 395, 1841
AccB7I CCANNNNNTGG 1 cut(s) 3905
AccBSI CCGCTC 2 cut(s) 275, 331
AccI GTMKAC 1 cut(s) 150
AccII CGCG 1 cut(s) 155
AccIII TCCGGA 1 cut(s) 2511
AciI CCGC 9 cut(s) 45, 160, 222, 273, 329, 423, 686, 1052, 3304
AclI AACGTT 1 cut(s) 3218
AclWI GGATC 4 cut(s) 895, 1134, 1723, 2379
AcoI YGGCCR 4 cut(s) 788, 1071, 2974, 3765
AcsI RAATTY 9 cut(s) 560, 805, 1370, 1391, 1633, 2102, 2695, 2988, 3677
AcuI CTGAAG 6 cut(s) 1134, 1784, 2259, 2735, 2948, 3693
AcvI CACGTG 2 cut(s) 3188, 3905
AcyI GRCGYC 2 cut(s) 291, 396
AdeI CACNNNGTG 1 cut(s) 1454
AfiI CCNNNNNNNGG 4 cut(s) 237, 992, 2394, 3905
AflIII ACRYGT 2 cut(s) 153, 2954
AgeI ACCGGT 1 cut(s) 458
AhlI ACTAGT 1 cut(s) 2998
AjnI CCWGG 4 cut(s) 848, 1567, 2869, 3873
AjuI GAANNNNNNNTTGG 2 cut(s) 2316, 2348
AleI CACNNNNGTG 1 cut(s) 459
AloI GAACNNNNNNTCC 2 cut(s) 72, 104
Alw21I GWGCWC 5 cut(s) 1340, 2291, 2551, 2986, 3828
Alw44I GTGCAC 2 cut(s) 2287, 2982
AlwI GGATC 4 cut(s) 895, 1134, 1723, 2379
AlwNI CAGNNNCTG 4 cut(s) 1484, 2537, 2768, 3980
Ama87I CYCGRG 1 cut(s) 391
Aor13HI TCCGGA 1 cut(s) 2511
AoxI GGCC 7 cut(s) 247, 788, 800, 1071, 1643, 2974, 3765
ApaLI GTGCAC 2 cut(s) 2287, 2982
ApeKI GCWGC 8 cut(s) 691, 2072, 2269, 2303, 2354, 2765, 3878, 4041
ApoI RAATTY 9 cut(s) 560, 805, 1370, 1391, 1633, 2102, 2695, 2988, 3677
ArsI GACNNNNNNTTYG 6 cut(s) 2443, 2475, 3251, 3283, 3676, 3708
AseI ATTAAT 1 cut(s) 3069
AsiGI ACCGGT 1 cut(s) 458
Asp700I GAANNNNTTC 4 cut(s) 1269, 2699, 3678, 3996
Asp718I GGTACC 1 cut(s) 1841
AspLEI GCGC 2 cut(s) 398, 2757
AspS9I GGNCC 4 cut(s) 22, 240, 1643, 3009
AsuC2I CCSGG 2 cut(s) 392, 393
AsuHPI GGTGA 7 cut(s) 473, 791, 1458, 1736, 1756, 2438, 3863
AsuII TTCGAA 1 cut(s) 2629
AvaI CYCGRG 1 cut(s) 391
AvaII GGWCC 3 cut(s) 22, 240, 3009
BaeGI GKGCMC 2 cut(s) 2291, 2986
BaeI ACNNNNGTAYC 4 cut(s) 1164, 1197, 2623, 2656
BalI TGGCCA 4 cut(s) 790, 1073, 2976, 3767
BanI GGYRCC 2 cut(s) 395, 1841
BanII GRGCYC 3 cut(s) 998, 1340, 3828
BarI GAAGNNNNNNTAC 2 cut(s) 3961, 3993
BbrPI CACGTG 2 cut(s) 3188, 3905
BbsI GAAGAC 2 cut(s) 272, 3247
Bbv12I GWGCWC 5 cut(s) 1340, 2291, 2551, 2986, 3828
BbvCI CCTCAGC 1 cut(s) 3795
BbvI GCAGC 7 cut(s) 678, 2059, 2256, 2290, 2366, 2777, 3865
BceAI ACGGC 7 cut(s) 21, 131, 235, 383, 512, 1279, 1409
BciT130I CCWGG 4 cut(s) 850, 1569, 2871, 3875
BciVI GTATCC 1 cut(s) 532
BclI TGATCA 1 cut(s) 1399
BcnI CCSGG 2 cut(s) 392, 393
BcuI ACTAGT 1 cut(s) 2998
BfaI CTAG 7 cut(s) 491, 603, 1322, 2162, 2183, 2999, 3890
BfmI CTRYAG 6 cut(s) 916, 987, 2112, 2304, 2727, 2769
BfoI RGCGCY 2 cut(s) 399, 2758
BfuAI ACCTGC 3 cut(s) 1469, 1902, 2677
BfuI GTATCC 1 cut(s) 532
BglII AGATCT 4 cut(s) 1324, 2419, 3061, 3759
BisI GCNGC 9 cut(s) 329, 692, 2073, 2270, 2304, 2355, 2766, 3879, 4042
BlsI GCNGC 9 cut(s) 330, 693, 2074, 2271, 2305, 2356, 2767, 3880, 4043
Bme1390I CCNGG 6 cut(s) 392, 393, 850, 1569, 2871, 3875
Bme18I GGWCC 3 cut(s) 22, 240, 3009
BmeT110I CYCGRG 1 cut(s) 391
BmgT120I GGNCC 4 cut(s) 22, 240, 1643, 3009
BmiI GGNNCC 7 cut(s) 242, 321, 397, 997, 1843, 2868, 3010
BmrFI CCNGG 6 cut(s) 392, 393, 850, 1569, 2871, 3875
BpiI GAAGAC 2 cut(s) 272, 3247
BplI GAGNNNNNCTC 2 cut(s) 1430, 1462
BpmI CTGGAG 3 cut(s) 2149, 2381, 3849
Bpu10I CCTNAGC 3 cut(s) 1339, 3795, 3822
Bpu14I TTCGAA 1 cut(s) 2629
BpuEI CTTGAG 3 cut(s) 1111, 2421, 2664
BpuMI CCSGG 2 cut(s) 392, 393
BsaAI YACGTR 4 cut(s) 667, 2639, 3188, 3905
BsaBI GATNNNNATC 1 cut(s) 381
BsaHI GRCGYC 2 cut(s) 291, 396
BsaI GGTCTC 2 cut(s) 230, 1433
BsaJI CCNNGG 5 cut(s) 244, 391, 849, 1567, 3369
BsaWI WCCGGW 2 cut(s) 458, 2511
BsaXI ACNNNNNCTCC 6 cut(s) 72, 102, 191, 221, 3847, 3877
Bsc4I CCNNNNNNNGG 4 cut(s) 237, 992, 2394, 3905
Bse118I RCCGGY 2 cut(s) 167, 458
Bse1I ACTGG 7 cut(s) 877, 1029, 2364, 2542, 3120, 3408, 3866
Bse3DI GCAATG 4 cut(s) 385, 1822, 2140, 2655
Bse8I GATNNNNATC 1 cut(s) 381
BseAI TCCGGA 1 cut(s) 2511
BseBI CCWGG 4 cut(s) 850, 1569, 2871, 3875
BseDI CCNNGG 5 cut(s) 244, 391, 849, 1567, 3369
BseJI GATNNNNATC 1 cut(s) 381
BseLI CCNNNNNNNGG 4 cut(s) 237, 992, 2394, 3905
BseMI GCAATG 4 cut(s) 385, 1822, 2140, 2655
BseMII CTCAG 5 cut(s) 1353, 1428, 3786, 3813, 4019
BseNI ACTGG 7 cut(s) 877, 1029, 2364, 2542, 3120, 3408, 3866
BseRI GAGGAG 5 cut(s) 836, 1197, 2463, 2836, 3812
BseSI GKGCMC 2 cut(s) 2291, 2986
BseXI GCAGC 7 cut(s) 678, 2059, 2256, 2290, 2366, 2777, 3865
BsgI GTGCAG 3 cut(s) 1725, 2490, 3867
Bsh1236I CGCG 1 cut(s) 155
Bsh1285I CGRYCG 1 cut(s) 1381
BshFI GGCC 7 cut(s) 249, 790, 802, 1073, 1645, 2976, 3767
BshNI GGYRCC 2 cut(s) 395, 1841
BshTI ACCGGT 1 cut(s) 458
BsiEI CGRYCG 1 cut(s) 1381
BsiHKAI GWGCWC 5 cut(s) 1340, 2291, 2551, 2986, 3828
BsiHKCI CYCGRG 1 cut(s) 391
BsiSI CCGG 4 cut(s) 168, 392, 459, 2512
BslFI GGGAC 6 cut(s) 226, 851, 1361, 2389, 3575, 3767
BslI CCNNNNNNNGG 4 cut(s) 237, 992, 2394, 3905
BsmBI CGTCTC 2 cut(s) 187, 307
BsmFI GGGAC 6 cut(s) 226, 851, 1361, 2389, 3575, 3767
BsmI GAATGC 1 cut(s) 3154
BsnI GGCC 7 cut(s) 249, 790, 802, 1073, 1645, 2976, 3767
Bso31I GGTCTC 2 cut(s) 230, 1433
BsoBI CYCGRG 1 cut(s) 391
Bsp119I TTCGAA 1 cut(s) 2629
Bsp1286I GDGCHC 6 cut(s) 998, 1340, 2291, 2551, 2986, 3828
Bsp13I TCCGGA 1 cut(s) 2511
Bsp1407I TGTACA 3 cut(s) 2044, 2403, 2524
BspACI CCGC 9 cut(s) 45, 160, 222, 273, 329, 423, 686, 1052, 3304
BspANI GGCC 7 cut(s) 249, 790, 802, 1073, 1645, 2976, 3767
BspCNI CTCAG 5 cut(s) 1352, 1429, 3787, 3814, 4018
BspEI TCCGGA 1 cut(s) 2511
BspFNI CGCG 1 cut(s) 155
BspHI TCATGA 2 cut(s) 1396, 2823
BspLI GGNNCC 7 cut(s) 242, 321, 397, 997, 1843, 2868, 3010
BspMAI CTGCAG 1 cut(s) 2308
BspMI ACCTGC 3 cut(s) 1469, 1902, 2677
BspPI GGATC 4 cut(s) 895, 1134, 1723, 2379
BspT104I TTCGAA 1 cut(s) 2629
BspT107I GGYRCC 2 cut(s) 395, 1841
BspTNI GGTCTC 2 cut(s) 230, 1433
BsrBI CCGCTC 2 cut(s) 275, 331
BsrDI GCAATG 4 cut(s) 385, 1822, 2140, 2655
BsrFI RCCGGY 2 cut(s) 167, 458
BsrGI TGTACA 3 cut(s) 2044, 2403, 2524
BsrI ACTGG 7 cut(s) 877, 1029, 2364, 2542, 3120, 3408, 3866
BssAI RCCGGY 2 cut(s) 167, 458
BssECI CCNNGG 5 cut(s) 244, 391, 849, 1567, 3369
BssNI GRCGYC 2 cut(s) 291, 396
BssT1I CCWWGG 2 cut(s) 244, 3369
Bst2UI CCWGG 4 cut(s) 850, 1569, 2871, 3875
Bst6I CTCTTC 2 cut(s) 32, 310
BstACI GRCGYC 2 cut(s) 291, 396
BstAPI GCANNNNNTGC 1 cut(s) 3395
BstAUI TGTACA 3 cut(s) 2044, 2403, 2524
BstBAI YACGTR 4 cut(s) 667, 2639, 3188, 3905
BstBI TTCGAA 1 cut(s) 2629
BstDEI CTNAG 6 cut(s) 944, 1339, 1437, 3795, 3822, 4005
BstFNI CGCG 1 cut(s) 155
BstH2I RGCGCY 2 cut(s) 399, 2758
BstHHI GCGC 2 cut(s) 398, 2757
BstMCI CGRYCG 1 cut(s) 1381
BstMWI GCNNNNNNNGC 7 cut(s) 44, 152, 168, 337, 3310, 3395, 3800
BstNI CCWGG 4 cut(s) 850, 1569, 2871, 3875
BstNSI RCATGY 8 cut(s) 758, 1028, 2127, 2147, 2373, 2445, 2958, 3657
BstSCI CCNGG 6 cut(s) 390, 391, 848, 1567, 2869, 3873
BstSFI CTRYAG 6 cut(s) 916, 987, 2112, 2304, 2727, 2769
BstSLI GKGCMC 2 cut(s) 2291, 2986
BstSNI TACGTA 1 cut(s) 2639
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 7 cut(s) 678, 2059, 2256, 2290, 2366, 2777, 3865
BstV2I GAAGAC 2 cut(s) 272, 3247
BstX2I RGATCY 6 cut(s) 900, 1324, 2384, 2419, 3061, 3759
BstYI RGATCY 6 cut(s) 900, 1324, 2384, 2419, 3061, 3759
BsuI GTATCC 1 cut(s) 532
BsuRI GGCC 7 cut(s) 249, 790, 802, 1073, 1645, 2976, 3767
BtsI GCAGTG 2 cut(s) 2551, 3108
BveI ACCTGC 3 cut(s) 1469, 1902, 2677
CaiI CAGNNNCTG 4 cut(s) 1484, 2537, 2768, 3980
CciI TCATGA 2 cut(s) 1396, 2823
CfoI GCGC 2 cut(s) 398, 2757
Cfr10I RCCGGY 2 cut(s) 167, 458
Cfr13I GGNCC 4 cut(s) 22, 240, 1643, 3009
Cfr9I CCCGGG 1 cut(s) 391
CseI GACGC 4 cut(s) 161, 2375, 3235, 3676
CspAI ACCGGT 1 cut(s) 458
DdeI CTNAG 6 cut(s) 944, 1339, 1437, 3795, 3822, 4005
DinI GGCGCC 1 cut(s) 397
DraIII CACNNNGTG 1 cut(s) 1454
EaeI YGGCCR 4 cut(s) 788, 1071, 2974, 3765
Eam1104I CTCTTC 2 cut(s) 32, 310
EarI CTCTTC 2 cut(s) 32, 310
Ecl136II GAGCTC 2 cut(s) 1338, 3826
Eco105I TACGTA 1 cut(s) 2639
Eco130I CCWWGG 2 cut(s) 244, 3369
Eco147I AGGCCT 1 cut(s) 249
Eco24I GRGCYC 3 cut(s) 998, 1340, 3828
Eco31I GGTCTC 2 cut(s) 230, 1433
Eco47I GGWCC 3 cut(s) 22, 240, 3009
Eco53kI GAGCTC 2 cut(s) 1338, 3826
Eco57I CTGAAG 6 cut(s) 1134, 1784, 2259, 2735, 2948, 3693
Eco72I CACGTG 2 cut(s) 3188, 3905
Eco88I CYCGRG 1 cut(s) 391
EcoICRI GAGCTC 2 cut(s) 1338, 3826
EcoRI GAATTC 2 cut(s) 1633, 2695
EcoRII CCWGG 4 cut(s) 848, 1567, 2869, 3873
EcoT14I CCWWGG 2 cut(s) 244, 3369
EcoT22I ATGCAT 3 cut(s) 2125, 3211, 3454
EcoT38I GRGCYC 3 cut(s) 998, 1340, 3828
EgeI GGCGCC 1 cut(s) 397
EheI GGCGCC 1 cut(s) 397
ErhI CCWWGG 2 cut(s) 244, 3369
Esp3I CGTCTC 2 cut(s) 187, 307
FaqI GGGAC 6 cut(s) 226, 851, 1361, 2389, 3575, 3767
FauI CCCGC 2 cut(s) 430, 1045
FbaI TGATCA 1 cut(s) 1399
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 9 cut(s) 329, 692, 2073, 2270, 2304, 2355, 2766, 3879, 4042
FriOI GRGCYC 3 cut(s) 998, 1340, 3828
Fsp4HI GCNGC 9 cut(s) 329, 692, 2073, 2270, 2304, 2355, 2766, 3879, 4042
FspBI CTAG 7 cut(s) 491, 603, 1322, 2162, 2183, 2999, 3890
GlaI GCGC 2 cut(s) 397, 2756
GluI GCNGC 9 cut(s) 329, 692, 2073, 2270, 2304, 2355, 2766, 3879, 4042
GsuI CTGGAG 3 cut(s) 2149, 2381, 3849
HaeII RGCGCY 2 cut(s) 399, 2758
HaeIII GGCC 7 cut(s) 249, 790, 802, 1073, 1645, 2976, 3767
HapII CCGG 4 cut(s) 168, 392, 459, 2512
HgaI GACGC 4 cut(s) 161, 2375, 3235, 3676
HhaI GCGC 2 cut(s) 398, 2757
Hin1I GRCGYC 2 cut(s) 291, 396
Hin6I GCGC 2 cut(s) 396, 2755
HinP1I GCGC 2 cut(s) 396, 2755
HincII GTYRAC 5 cut(s) 151, 1245, 1603, 3045, 3238
HindII GTYRAC 5 cut(s) 151, 1245, 1603, 3045, 3238
HindIII AAGCTT 3 cut(s) 2747, 2946, 3292
HpaI GTTAAC 2 cut(s) 1245, 1603
HpaII CCGG 4 cut(s) 168, 392, 459, 2512
HphI GGTGA 7 cut(s) 473, 791, 1458, 1736, 1756, 2438, 3863
Hpy99I CGWCG 3 cut(s) 152, 155, 2072
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 152, 168, 337, 3310, 3395, 3800
HpyF3I CTNAG 6 cut(s) 944, 1339, 1437, 3795, 3822, 4005
Hsp92I GRCGYC 2 cut(s) 291, 396
HspAI GCGC 2 cut(s) 396, 2755
KasI GGCGCC 1 cut(s) 395
Kpn2I TCCGGA 1 cut(s) 2511
KpnI GGTACC 1 cut(s) 1845
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Ksp22I TGATCA 1 cut(s) 1399
KspAI GTTAAC 2 cut(s) 1245, 1603
Lsp1109I GCAGC 7 cut(s) 678, 2059, 2256, 2290, 2366, 2777, 3865
MaeI CTAG 7 cut(s) 491, 603, 1322, 2162, 2183, 2999, 3890
MbiI CCGCTC 2 cut(s) 275, 331
MfeI CAATTG 2 cut(s) 235, 3594
MflI RGATCY 6 cut(s) 900, 1324, 2384, 2419, 3061, 3759
MhlI GDGCHC 6 cut(s) 998, 1340, 2291, 2551, 2986, 3828
MlsI TGGCCA 4 cut(s) 790, 1073, 2976, 3767
MluI ACGCGT 1 cut(s) 153
MluNI TGGCCA 4 cut(s) 790, 1073, 2976, 3767
Mly113I GGCGCC 1 cut(s) 396
MlyI GAGTC 1 cut(s) 3050
MmeI TCCRAC 3 cut(s) 1989, 3112, 3201
Mox20I TGGCCA 4 cut(s) 790, 1073, 2976, 3767
Mph1103I ATGCAT 3 cut(s) 2125, 3211, 3454
MroI TCCGGA 1 cut(s) 2511
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 4 cut(s) 1269, 2699, 3678, 3996
MscI TGGCCA 4 cut(s) 790, 1073, 2976, 3767
MslI CAYNNNNRTG 2 cut(s) 459, 2316
Msp20I TGGCCA 4 cut(s) 790, 1073, 2976, 3767
MspA1I CMGCKG 3 cut(s) 2768, 3794, 4044
MspI CCGG 4 cut(s) 168, 392, 459, 2512
MspR9I CCNGG 6 cut(s) 392, 393, 850, 1569, 2871, 3875
MunI CAATTG 2 cut(s) 235, 3594
Mva1269I GAATGC 1 cut(s) 3154
MvaI CCWGG 4 cut(s) 850, 1569, 2871, 3875
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 7 cut(s) 44, 152, 168, 337, 3310, 3395, 3800
NaeI GCCGGC 1 cut(s) 169
NarI GGCGCC 1 cut(s) 396
NciI CCSGG 2 cut(s) 392, 393
NgoMIV GCCGGC 1 cut(s) 167
NlaIV GGNNCC 7 cut(s) 242, 321, 397, 997, 1843, 2868, 3010
NmuCI GTSAC 8 cut(s) 1303, 1363, 1454, 1762, 2085, 2347, 3103, 3943
NsiI ATGCAT 3 cut(s) 2125, 3211, 3454
NspI RCATGY 8 cut(s) 758, 1028, 2127, 2147, 2373, 2445, 2958, 3657
NspV TTCGAA 1 cut(s) 2629
OliI CACNNNNGTG 1 cut(s) 459
PaeI GCATGC 1 cut(s) 2127
PagI TCATGA 2 cut(s) 1396, 2823
PceI AGGCCT 1 cut(s) 249
PciI ACATGT 1 cut(s) 2954
PcsI WCGNNNNNNNCGW 2 cut(s) 297, 2635
PctI GAATGC 1 cut(s) 3154
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 4 cut(s) 1269, 2699, 3678, 3996
PfeI GAWTC 9 cut(s) 14, 584, 982, 1223, 1820, 2572, 2841, 2901, 3921
PflMI CCANNNNNTGG 1 cut(s) 3905
PfoI TCCNGGA 1 cut(s) 2869
PinAI ACCGGT 1 cut(s) 458
PkrI GCNGC 9 cut(s) 330, 693, 2074, 2271, 2305, 2356, 2767, 3880, 4043
Ple19I CGATCG 1 cut(s) 1381
PleI GAGTC 1 cut(s) 3049
PluTI GGCGCC 1 cut(s) 399
PmaCI CACGTG 2 cut(s) 3188, 3905
PmlI CACGTG 2 cut(s) 3188, 3905
PpsI GAGTC 1 cut(s) 3049
Ppu21I YACGTR 4 cut(s) 667, 2639, 3188, 3905
PscI ACATGT 1 cut(s) 2954
PshBI ATTAAT 1 cut(s) 3069
Psp124BI GAGCTC 2 cut(s) 1340, 3828
Psp1406I AACGTT 1 cut(s) 3218
Psp6I CCWGG 4 cut(s) 848, 1567, 2869, 3873
PspCI CACGTG 2 cut(s) 3188, 3905
PspGI CCWGG 4 cut(s) 848, 1567, 2869, 3873
PspN4I GGNNCC 7 cut(s) 242, 321, 397, 997, 1843, 2868, 3010
PspPI GGNCC 4 cut(s) 22, 240, 1643, 3009
PstI CTGCAG 1 cut(s) 2308
PstNI CAGNNNCTG 4 cut(s) 1484, 2537, 2768, 3980
PsuI RGATCY 6 cut(s) 900, 1324, 2384, 2419, 3061, 3759
PvuI CGATCG 1 cut(s) 1381
PvuII CAGCTG 3 cut(s) 2768, 3794, 4044
RseI CAYNNNNRTG 2 cut(s) 459, 2316
SacI GAGCTC 2 cut(s) 1340, 3828
SalI GTCGAC 1 cut(s) 149
SatI GCNGC 9 cut(s) 329, 692, 2073, 2270, 2304, 2355, 2766, 3879, 4042
Sau96I GGNCC 4 cut(s) 22, 240, 1643, 3009
SchI GAGTC 1 cut(s) 3050
ScrFI CCNGG 6 cut(s) 392, 393, 850, 1569, 2871, 3875
SduI GDGCHC 6 cut(s) 998, 1340, 2291, 2551, 2986, 3828
SfcI CTRYAG 6 cut(s) 916, 987, 2112, 2304, 2727, 2769
SfoI GGCGCC 1 cut(s) 397
SfuI TTCGAA 1 cut(s) 2629
SgrAI CRCCGGYG 1 cut(s) 458
SgrDI CGTCGACG 1 cut(s) 149
SinI GGWCC 3 cut(s) 22, 240, 3009
SmaI CCCGGG 1 cut(s) 393
SmiMI CAYNNNNRTG 2 cut(s) 459, 2316
SmlI CTYRAG 3 cut(s) 1090, 2436, 2679
SmoI CTYRAG 3 cut(s) 1090, 2436, 2679
SnaBI TACGTA 1 cut(s) 2639
SpeI ACTAGT 1 cut(s) 2998
SphI GCATGC 1 cut(s) 2127
SrfI GCCCGGGC 1 cut(s) 393
SseBI AGGCCT 1 cut(s) 249
SsiI CCGC 9 cut(s) 45, 160, 222, 273, 329, 423, 686, 1052, 3304
SspDI GGCGCC 1 cut(s) 395
SspI AATATT 2 cut(s) 1239, 1546
SspMI CTAG 7 cut(s) 491, 603, 1322, 2162, 2183, 2999, 3890
SstI GAGCTC 2 cut(s) 1340, 3828
StuI AGGCCT 1 cut(s) 249
StyD4I CCNGG 6 cut(s) 390, 391, 848, 1567, 2869, 3873
StyI CCWWGG 2 cut(s) 244, 3369
TaqI TCGA 8 cut(s) 150, 676, 1655, 2551, 2629, 3050, 3258, 3919
TatI WGTACW 5 cut(s) 1200, 2044, 2403, 2524, 2788
TauI GCSGC 1 cut(s) 331
TfiI GAWTC 9 cut(s) 14, 584, 982, 1223, 1820, 2572, 2841, 2901, 3921
TseFI GTSAC 8 cut(s) 1303, 1363, 1454, 1762, 2085, 2347, 3103, 3943
TseI GCWGC 8 cut(s) 691, 2072, 2269, 2303, 2354, 2765, 3878, 4041
Tsp45I GTSAC 8 cut(s) 1303, 1363, 1454, 1762, 2085, 2347, 3103, 3943
TspGWI ACGGA 4 cut(s) 35, 169, 723, 743
TspMI CCCGGG 1 cut(s) 391
Van91I CCANNNNNTGG 1 cut(s) 3905
VneI GTGCAC 2 cut(s) 2287, 2982
VpaK11BI GGWCC 3 cut(s) 22, 240, 3009
VspI ATTAAT 1 cut(s) 3069
XapI RAATTY 9 cut(s) 560, 805, 1370, 1391, 1633, 2102, 2695, 2988, 3677
XceI RCATGY 8 cut(s) 758, 1028, 2127, 2147, 2373, 2445, 2958, 3657
XcmI CCANNNNNNNNNTGG 2 cut(s) 2803, 3600
XmaI CCCGGG 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 150
XmnI GAANNNNTTC 4 cut(s) 1269, 2699, 3678, 3996
XspI CTAG 7 cut(s) 491, 603, 1322, 2162, 2183, 2999, 3890
ZraI GACGTC 1 cut(s) 292
Zsp2I ATGCAT 3 cut(s) 2125, 3211, 3454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.