Rh3DG348600

DNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0017511
Physical Location & Seq
Reverse (-)
39715971 .. 39728080
12110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG348600.1

Sequence Viewer

Length: 4194 bp
ATGACGGCTCTGGATTCTACGGACCTCTTCCTGACTGCCCTAACCGCCTTTTCCCACCCACCCAGACCCAGACCCCTTGAACTCTCTCTCCCTCCAATTCCCTCCACATTCCCCCACTCCAAACTCATCCCCAGAACCCGTTTCGCCGTCGACGCGTTCCGCTATGCCGGCGATTATTCCGTCTCTTACTTCCTCTCTCACTTCCACTCCGATCACTACGGCGGTCTCTCCCCCAATTGGTCCCAAGGCCTCGTCTTTTGCTCCCCCACCACCGCTCGTCTTCTCACAGACGTCCTCAACGTCTCTTCGCTTTTCGTGGTTCCTCTGCCGCTCGGACAGCCCCTTCTCATTGATGGATGCGAGGTCGTGCTTATTGACGCCAATCATTGCCCGGGCGCCGTTCAGTTCTTGTTCAAAGTTCCCGCCTCCAATGGAGACTTGCAGAGGTATGTTCACACCGGTGATTTTCGGTTTTCTGCTCCGATGAAGCTAGACCCTTTTCTCTGTAACTTTGTGGGTTCAGATGCCGTTTTCTTGGATACAACTTATTGCAATCCCAAATTCGTGTTTCCTTCACAAGAAGAATCTGTTGATTATATTTCTAGCTTGATAGAAAGAGTCGGAGGTGGACACCAAGTTTCTGTGAAAAGTGTTCTGTTCCTTGTTGCTACATATGTGATTGGGAAAGAGAAGATTTTGATAGAAATTGCTCGTAGATGTAAGCGTAAAGTGTACGTGGATGCTCGAAAAATGGCGGTGCTGCGTGTTTTGGGTTACGGAGACAGTGGGGTGTTTACGGAGGATGAATGTGAGACTGATGTGCATGTTGTTGGGTGGAATGTGTTGGGTGAGACTTGGCCATATTTCAGGCCAAATTTTGTGAAAATGGAGGAGGTTATGGTTGAGAGGGGGTATTCCAGGGTTGTAGGGTTCGTCCCTACTGGCTGGACTTATGAAGTCAAGCGTAACAAGTTCTCGGTGAGGTCAAAGGATGCTTTTGAGATCCATCTTGTACCCTACAGTGAACATTCAAACTATGATGAACTTAGAGAATACGTCAGGTTTTTGAAACCAAAGCGTGTGATTCCTACAGCGGGCTCCGATGTGGAAAAGATAGACAGTAAACATGCCAGTAAAATGCAAAAGCATTTTGCTGGGTTAATTGATGAAATGGCCAACAAAAAAGAGTTCTTGAGGGGTTTTCATCGTGAGACTACAGAAGGAGGTGGCAAGGTTCTCAGTGATGCCAAAGATTACACGATGGAGGGGCAGGATCAAGAGAAAAAGGCAACACCTTCAGATATTGATACTATGGGAGATACTAATGTAGGCTTTGGTAAGGAATTGTACTCTCCTCTCCAAGAACCTGATTCACAAACTCCAATATTGTTAACTGATGAAAAAGCCGAGGAAATTATTCAACAACTTCGTGATTGTTTGCCGTCATGGGTCACTCGCCAGCAGATGCTAGATCTGATTGGGAGCTCAGGAGGGGACATTGTTGAAGCAGTCACTAAATTTTACGATCGTGAAACTGAATTTCATGATCAAGGAATTGCCTCTACAAGTGCCGTATCTGTATCTGAGACCAGCTCACTCTGTGACACAGCATCACCGACAAAAGCAGGTTCTGTTCATGCAAATATTAATGTTTCTTCAAGCCAAGCTCATATTTCACCCAACCCAAGAAATATTGTTAAAAGTGGCATTTCCCCTGGAAAAAGAGGGAGGAAGATCAGTAATAAAGTTAACAAGAAAGTAAAGTTACAGTCAAAACTGAATTCGTGTGGGCCAAAACAATCGACAATAACAAGGTTTTTTAGTAAAGTTTTACCTGACGTTACAGAAAGTGTAGAGATTGGATCAGTAGGTGAACAAAACCATAAAGATAAAAAATTACCAGATCATGTCACCCAATCATACAAGGATGCAATAGATCAATTTCTTCAGATAATAGATGGCAATGAATCATTAAAAAGCTATGCTGGTACCATTCTTCAAAAGGCAAATGGGGATATTAGTATGGCTGTGGATATACATTACCGTAATAATGAAGGCAGGTCTGATGAGAATGAAGTGGAGCTGGTGGTTGATGCAAAATCAGTTCAGTCCAACTGTGGTATAGATAACTGTTCTTCTGTCAAAAAAAAAATTGAATTAGGGAAAATAGGAGTGGTAGCTGATTTGTCTGTACAGGGGTCATTACCAGATAACGTCGCTGCAACTTCTGTGTCACTACCACCAGAAAAATTCAATCCTGTAGATCATGCATGCTGGAGCAATGGACAACATGCTCCATATTTACATCTTGCACGAACCTTTGACTTACTAGAGGATGAAAAAGGCAAGATAAAGGCTACTTCTATGCTCTGCAATATGTTCAGAAGTTTGCTTGCCCTGTCTCCTGATGATGTGCTGCCTTCAGTTTATTTGTGCACCAATAAGATAGCTGCAGACCATGAAAATGTGGAACTGAACATTGGTGGAAGTTTGGTCACAGCAGCACTGGAGGACGCATGTGGGACAAGCAGATCCAAAATCAGGGATATGTACAATGAATTGGGAGATCTTGGTGATGTTGCTCAAGCATGTCGCCAGACACAGACTTTGCTTGCCCCTCCTCCACCACTTCTAATAAAAGATGTATTTTCTGCACTCCGGAAGATAAGTGTACAAACAGGTACTGGAAGCAGTGCTCGAAAGAGAAGCCTGATTGTGAATCTCTTGCGTTCTTGCAGAGAGAAAGAGATGAAGTTCCTTATTAGAACTTTGGTTCGAAACTTACGTATCGGAGCAATGATGAAAACTGTCCTACCTGCTCTGGCTCAAGCTGTTGTTCTGAATTCCTTCCATAGCTGTAATCACGAAGGAACTATAGAAAGTTTGAAGGACAAGCTTCAGCGCCATTCAGCAGCTGTAGTAGAAGCATACAATGTACTGCCCAATTTGGATTTGGTCGTTCCTTCTCTCATGAAAAGAGGCATTGGATTCTCCTCATCAACTTTGTCAATGGTTCCAGGAATACCTATCAAGCCAATGCTTGCAAGAATCACAAATGGTGTTCAGCAAGCACTGAAGCTATTTGAAAAGAAAGCTTTTACATGTGAATACAAATATGATGGCCAGCGTGCACAAATTCATAAACTAGTTGATGGGTCCATGCACGTTTTTACAAGAAATGGGGATGAGTCAACTTCGAGATTTCCAGATCTGATTAATATAATCAATCAATCTTGTAAACCCGATGCAGTGACTTTCATACTGGATGCAGAGATTGTTGGAGTTGATAGGAAGAATGCTTGCAAGCTAATGTCCTTCCAAGAATTATCTTCACGTGGGAGAGGGAGCAGAGATGCATCAATAACGTTGGATAGCATAAAGGTCAACATTTGCGTCTTTGTCTTCGACATCATGTTTGCCAATGGACAACAGTTGTTAAGCTTTCCTCTGCGGAGAAGGCGAAAGTATTTGAAAGAGTTGTTCTACGATGAGAAGTTGGGTTACTTTGAATATGCCAAGGAAATGACAGTTGAGGCAGAGGATGCTTGTTTAACCAGTGAAGCGACATTAACCAAAATAAACTCTTTCCTTGAAAATGCATTTGTTTCCTCATGTGAAGGGATTATAGTTAAATCTTTAGATGTTGATGCTGGATATTCTCCATCAAAGCGTACAGATACTTGGTTAAAGGTCAAGCGAGACTACATGGAGGGATTGGGCGATACTCTTGATTTAGTCCCAATTGGAGCTTGGCATGGAAACGGGAGAAAGGCAGGCTGGTACAGTCCATTTCTCATGGCATGTTACAATCCTGAAACTGAAGAATTTCAAAGCGTCTGTCGTGTTATGTCTGGGTTTTCAGATTTATTTTATACAGAGATGAAAAGTTTTTATTCTGGGGACAAGATCTTGGCCAAAAAACCACCTTATTATAGAACAGCTGAGCAGCCTGATAAGTGGTTTTCTCCTGAGCTCGTATGGGAAATAAGAGGTGCAGACTTTACAGTGTCTCCAGTTCACCAGGCAGCTTTAGGTCTAGTTCATCCATCACGTGGCATTTCCATTCGATTCCCAAGATACATTCGCACTGTGACTGATAGAAAACCAGATGAATGTAGCACAGCTTCTGATTTAGCTGAAATGTTTCATTCTCAGACGAGGAAGATGGATGTGGCTTCTGAAGATGGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000012 GO:0000726 GO:0002237 GO:0003006 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009314 GO:0009409 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010154 GO:0010225 GO:0010243 GO:0015074 GO:0016070 GO:0016874 GO:0016886 GO:0022414 GO:0022616 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0046677 GO:0048316 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051103 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0090351 GO:0140097 GO:1901360 GO:1901576 GO:1901652 GO:1901698 GO:1901700 GO:1904975 GO:2000683 GO:2000685
KEGG Pathways
Metabolic & Signaling
ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430
Pfam Domains
Protein Families
DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DRMBL

Protein Analysis

1397

Amino Acids

155.34

Weight (kDa)

6.65

Isoelectric Point (pI)

45.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 263 - 365 1.3e-19 DNA repair metallo-beta-lactamase
DNA_ligase_A_N PF04675 766 - 942 4.1e-43 DNA ligase N terminus
DNA_ligase_A_M PF01068 1012 - 1232 1.5e-46 ATP dependent DNA ligase domain
DNA_ligase_A_C PF04679 1258 - 1365 5.9e-17 ATP dependent DNA ligase C terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017511)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17360
rosa_chinensis RchiOBHm_Chr3g0493941
rosa_laevigata RLG00000022689
rosa_multiflora Rmu_co8383177.1_g000001 Rmu_sc0000568.1_g000023 Rmu_sc0010399.1_g000002
rosa_samantha Rh3AG313100 Rh3BG348300 Rh3BG349300 Rh3CG346100 Rh3DG348600
rosa_wichuraiana Rw3G027410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 294
Acc36I ACCTGC 3 cut(s) 1616, 2049, 2824
Acc65I GGTACC 1 cut(s) 1988
AccB1I GGYRCC 2 cut(s) 395, 1988
AccB7I CCANNNNNTGG 1 cut(s) 4052
AccBSI CCGCTC 2 cut(s) 275, 331
AccI GTMKAC 1 cut(s) 150
AccII CGCG 1 cut(s) 155
AccIII TCCGGA 1 cut(s) 2658
AciI CCGC 9 cut(s) 45, 160, 222, 273, 329, 423, 755, 1094, 3451
AclI AACGTT 1 cut(s) 3365
AclWI GGATC 4 cut(s) 997, 1281, 1870, 2526
AcoI YGGCCR 4 cut(s) 857, 1173, 3121, 3912
AcsI RAATTY 9 cut(s) 560, 874, 1517, 1538, 1780, 2249, 2842, 3135, 3824
AcuI CTGAAG 6 cut(s) 1281, 1931, 2406, 2882, 3095, 3840
AcvI CACGTG 2 cut(s) 3335, 4052
AcyI GRCGYC 3 cut(s) 291, 378, 396
AdeI CACNNNGTG 1 cut(s) 1601
AfiI CCNNNNNNNGG 4 cut(s) 237, 1094, 2541, 4052
AflIII ACRYGT 2 cut(s) 153, 3101
AgeI ACCGGT 1 cut(s) 458
AhlI ACTAGT 1 cut(s) 3145
AjnI CCWGG 4 cut(s) 917, 1714, 3016, 4020
AjuI GAANNNNNNNTTGG 2 cut(s) 2463, 2495
AleI CACNNNNGTG 1 cut(s) 459
AloI GAACNNNNNNTCC 2 cut(s) 72, 104
Alw21I GWGCWC 5 cut(s) 1487, 2438, 2698, 3133, 3975
Alw44I GTGCAC 2 cut(s) 2434, 3129
AlwI GGATC 4 cut(s) 997, 1281, 1870, 2526
AlwNI CAGNNNCTG 4 cut(s) 1631, 2684, 2915, 4127
Ama87I CYCGRG 1 cut(s) 391
Aor13HI TCCGGA 1 cut(s) 2658
AoxI GGCC 7 cut(s) 247, 857, 869, 1173, 1790, 3121, 3912
ApaLI GTGCAC 2 cut(s) 2434, 3129
ApeKI GCWGC 9 cut(s) 760, 2219, 2416, 2450, 2501, 2912, 3946, 4025, 4188
ApoI RAATTY 9 cut(s) 560, 874, 1517, 1538, 1780, 2249, 2842, 3135, 3824
ArsI GACNNNNNNTTYG 6 cut(s) 2590, 2622, 3398, 3430, 3823, 3855
AseI ATTAAT 2 cut(s) 1647, 3216
AsiGI ACCGGT 1 cut(s) 458
Asp700I GAANNNNTTC 4 cut(s) 1416, 2846, 3825, 4143
Asp718I GGTACC 1 cut(s) 1988
AspLEI GCGC 2 cut(s) 398, 2904
AspS9I GGNCC 4 cut(s) 22, 240, 1790, 3156
AsuC2I CCSGG 2 cut(s) 392, 393
AsuHPI GGTGA 9 cut(s) 473, 860, 991, 1605, 1668, 1883, 1903, 2585, 4010
AsuII TTCGAA 1 cut(s) 2776
AvaI CYCGRG 1 cut(s) 391
AvaII GGWCC 3 cut(s) 22, 240, 3156
BaeGI GKGCMC 2 cut(s) 2438, 3133
BaeI ACNNNNGTAYC 4 cut(s) 1311, 1344, 2770, 2803
BalI TGGCCA 4 cut(s) 859, 1175, 3123, 3914
BanI GGYRCC 2 cut(s) 395, 1988
BanII GRGCYC 3 cut(s) 1100, 1487, 3975
BarI GAAGNNNNNNTAC 4 cut(s) 3485, 3517, 4108, 4140
BbrPI CACGTG 2 cut(s) 3335, 4052
BbsI GAAGAC 2 cut(s) 272, 3394
Bbv12I GWGCWC 5 cut(s) 1487, 2438, 2698, 3133, 3975
BbvI GCAGC 8 cut(s) 747, 2206, 2403, 2437, 2513, 2924, 3958, 4037
BceAI ACGGC 7 cut(s) 21, 131, 235, 383, 512, 1426, 1556
BciT130I CCWGG 4 cut(s) 919, 1716, 3018, 4022
BciVI GTATCC 1 cut(s) 532
BclI TGATCA 1 cut(s) 1546
BcnI CCSGG 2 cut(s) 392, 393
BcuI ACTAGT 1 cut(s) 3145
BfaI CTAG 6 cut(s) 491, 603, 1469, 2330, 3146, 4037
BfmI CTRYAG 7 cut(s) 1018, 1089, 1215, 2259, 2451, 2874, 2916
BfoI RGCGCY 2 cut(s) 399, 2905
BfuAI ACCTGC 3 cut(s) 1616, 2049, 2824
BfuI GTATCC 1 cut(s) 532
BglII AGATCT 4 cut(s) 1471, 2566, 3208, 3906
BlpI GCTNAGC 1 cut(s) 3942
Bme1390I CCNGG 6 cut(s) 392, 393, 919, 1716, 3018, 4022
Bme18I GGWCC 3 cut(s) 22, 240, 3156
BmeT110I CYCGRG 1 cut(s) 391
BmgT120I GGNCC 4 cut(s) 22, 240, 1790, 3156
BmiI GGNNCC 7 cut(s) 242, 321, 397, 1099, 1990, 3015, 3157
BmrFI CCNGG 6 cut(s) 392, 393, 919, 1716, 3018, 4022
BpiI GAAGAC 2 cut(s) 272, 3394
BplI GAGNNNNNCTC 2 cut(s) 1577, 1609
BpmI CTGGAG 3 cut(s) 2296, 2528, 3996
Bpu10I CCTNAGC 2 cut(s) 1486, 3969
Bpu1102I GCTNAGC 1 cut(s) 3942
Bpu14I TTCGAA 1 cut(s) 2776
BpuEI CTTGAG 3 cut(s) 1213, 2568, 2811
BpuMI CCSGG 2 cut(s) 392, 393
BsaAI YACGTR 4 cut(s) 736, 2786, 3335, 4052
BsaHI GRCGYC 3 cut(s) 291, 378, 396
BsaI GGTCTC 2 cut(s) 230, 1580
BsaJI CCNNGG 6 cut(s) 244, 391, 918, 1407, 1714, 3516
BsaWI WCCGGW 2 cut(s) 458, 2658
BsaXI ACNNNNNCTCC 6 cut(s) 72, 102, 191, 221, 3994, 4024
Bsc4I CCNNNNNNNGG 4 cut(s) 237, 1094, 2541, 4052
Bse118I RCCGGY 2 cut(s) 167, 458
Bse1I ACTGG 7 cut(s) 946, 1131, 2511, 2689, 3267, 3555, 4013
Bse3DI GCAATG 4 cut(s) 385, 1969, 2287, 2802
BseAI TCCGGA 1 cut(s) 2658
BseBI CCWGG 4 cut(s) 919, 1716, 3018, 4022
BseDI CCNNGG 6 cut(s) 244, 391, 918, 1407, 1714, 3516
BseLI CCNNNNNNNGG 4 cut(s) 237, 1094, 2541, 4052
BseMI GCAATG 4 cut(s) 385, 1969, 2287, 2802
BseMII CTCAG 6 cut(s) 1252, 1500, 1575, 3933, 3960, 4166
BseNI ACTGG 7 cut(s) 946, 1131, 2511, 2689, 3267, 3555, 4013
BseRI GAGGAG 4 cut(s) 905, 1344, 2610, 2983
BseSI GKGCMC 2 cut(s) 2438, 3133
BseXI GCAGC 8 cut(s) 747, 2206, 2403, 2437, 2513, 2924, 3958, 4037
BseYI CCCAGC 1 cut(s) 1154
BsgI GTGCAG 2 cut(s) 2637, 4014
Bsh1236I CGCG 1 cut(s) 155
Bsh1285I CGRYCG 1 cut(s) 1528
BshFI GGCC 7 cut(s) 249, 859, 871, 1175, 1792, 3123, 3914
BshNI GGYRCC 2 cut(s) 395, 1988
BshTI ACCGGT 1 cut(s) 458
BsiEI CGRYCG 1 cut(s) 1528
BsiHKAI GWGCWC 5 cut(s) 1487, 2438, 2698, 3133, 3975
BsiHKCI CYCGRG 1 cut(s) 391
BsiSI CCGG 4 cut(s) 168, 392, 459, 2659
BslFI GGGAC 6 cut(s) 226, 920, 1508, 2536, 3722, 3914
BslI CCNNNNNNNGG 4 cut(s) 237, 1094, 2541, 4052
BsmBI CGTCTC 2 cut(s) 187, 307
BsmFI GGGAC 6 cut(s) 226, 920, 1508, 2536, 3722, 3914
BsmI GAATGC 1 cut(s) 3301
BsnI GGCC 7 cut(s) 249, 859, 871, 1175, 1792, 3123, 3914
Bso31I GGTCTC 2 cut(s) 230, 1580
BsoBI CYCGRG 1 cut(s) 391
Bsp119I TTCGAA 1 cut(s) 2776
Bsp1286I GDGCHC 6 cut(s) 1100, 1487, 2438, 2698, 3133, 3975
Bsp13I TCCGGA 1 cut(s) 2658
Bsp1407I TGTACA 3 cut(s) 2191, 2550, 2671
Bsp1720I GCTNAGC 1 cut(s) 3942
BspACI CCGC 9 cut(s) 45, 160, 222, 273, 329, 423, 755, 1094, 3451
BspANI GGCC 7 cut(s) 249, 859, 871, 1175, 1792, 3123, 3914
BspCNI CTCAG 6 cut(s) 1251, 1499, 1576, 3934, 3961, 4165
BspEI TCCGGA 1 cut(s) 2658
BspFNI CGCG 1 cut(s) 155
BspHI TCATGA 2 cut(s) 1543, 2970
BspLI GGNNCC 7 cut(s) 242, 321, 397, 1099, 1990, 3015, 3157
BspMAI CTGCAG 1 cut(s) 2455
BspMI ACCTGC 3 cut(s) 1616, 2049, 2824
BspPI GGATC 4 cut(s) 997, 1281, 1870, 2526
BspT104I TTCGAA 1 cut(s) 2776
BspT107I GGYRCC 2 cut(s) 395, 1988
BspTNI GGTCTC 2 cut(s) 230, 1580
BsrBI CCGCTC 2 cut(s) 275, 331
BsrDI GCAATG 4 cut(s) 385, 1969, 2287, 2802
BsrFI RCCGGY 2 cut(s) 167, 458
BsrGI TGTACA 3 cut(s) 2191, 2550, 2671
BsrI ACTGG 7 cut(s) 946, 1131, 2511, 2689, 3267, 3555, 4013
BssAI RCCGGY 2 cut(s) 167, 458
BssECI CCNNGG 6 cut(s) 244, 391, 918, 1407, 1714, 3516
BssNI GRCGYC 3 cut(s) 291, 378, 396
BssT1I CCWWGG 2 cut(s) 244, 3516
Bst2UI CCWGG 4 cut(s) 919, 1716, 3018, 4022
Bst6I CTCTTC 2 cut(s) 32, 310
BstACI GRCGYC 3 cut(s) 291, 378, 396
BstAPI GCANNNNNTGC 1 cut(s) 3542
BstAUI TGTACA 3 cut(s) 2191, 2550, 2671
BstBAI YACGTR 4 cut(s) 736, 2786, 3335, 4052
BstBI TTCGAA 1 cut(s) 2776
BstDEI CTNAG 7 cut(s) 1046, 1238, 1486, 1584, 3942, 3969, 4152
BstFNI CGCG 1 cut(s) 155
BstH2I RGCGCY 2 cut(s) 399, 2905
BstHHI GCGC 2 cut(s) 398, 2904
BstMCI CGRYCG 1 cut(s) 1528
BstMWI GCNNNNNNNGC 6 cut(s) 44, 152, 168, 337, 3457, 3542
BstNI CCWGG 4 cut(s) 919, 1716, 3018, 4022
BstNSI RCATGY 8 cut(s) 827, 1130, 2274, 2294, 2520, 2592, 3105, 3804
BstSCI CCNGG 6 cut(s) 390, 391, 917, 1714, 3016, 4020
BstSFI CTRYAG 7 cut(s) 1018, 1089, 1215, 2259, 2451, 2874, 2916
BstSLI GKGCMC 2 cut(s) 2438, 3133
BstSNI TACGTA 1 cut(s) 2786
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 8 cut(s) 747, 2206, 2403, 2437, 2513, 2924, 3958, 4037
BstV2I GAAGAC 2 cut(s) 272, 3394
BstX2I RGATCY 6 cut(s) 1002, 1471, 2531, 2566, 3208, 3906
BstYI RGATCY 6 cut(s) 1002, 1471, 2531, 2566, 3208, 3906
BsuI GTATCC 1 cut(s) 532
BsuRI GGCC 7 cut(s) 249, 859, 871, 1175, 1792, 3123, 3914
BtsI GCAGTG 2 cut(s) 2698, 3255
BveI ACCTGC 3 cut(s) 1616, 2049, 2824
CaiI CAGNNNCTG 4 cut(s) 1631, 2684, 2915, 4127
CciI TCATGA 2 cut(s) 1543, 2970
CfoI GCGC 2 cut(s) 398, 2904
Cfr10I RCCGGY 2 cut(s) 167, 458
Cfr13I GGNCC 4 cut(s) 22, 240, 1790, 3156
Cfr9I CCCGGG 1 cut(s) 391
CseI GACGC 5 cut(s) 161, 386, 2522, 3382, 3823
CspAI ACCGGT 1 cut(s) 458
DdeI CTNAG 7 cut(s) 1046, 1238, 1486, 1584, 3942, 3969, 4152
DinI GGCGCC 1 cut(s) 397
DraIII CACNNNGTG 1 cut(s) 1601
EaeI YGGCCR 4 cut(s) 857, 1173, 3121, 3912
Eam1104I CTCTTC 2 cut(s) 32, 310
EarI CTCTTC 2 cut(s) 32, 310
Ecl136II GAGCTC 2 cut(s) 1485, 3973
Eco105I TACGTA 1 cut(s) 2786
Eco130I CCWWGG 2 cut(s) 244, 3516
Eco147I AGGCCT 1 cut(s) 249
Eco24I GRGCYC 3 cut(s) 1100, 1487, 3975
Eco31I GGTCTC 2 cut(s) 230, 1580
Eco47I GGWCC 3 cut(s) 22, 240, 3156
Eco53kI GAGCTC 2 cut(s) 1485, 3973
Eco57I CTGAAG 6 cut(s) 1281, 1931, 2406, 2882, 3095, 3840
Eco72I CACGTG 2 cut(s) 3335, 4052
Eco88I CYCGRG 1 cut(s) 391
EcoICRI GAGCTC 2 cut(s) 1485, 3973
EcoRI GAATTC 2 cut(s) 1780, 2842
EcoRII CCWGG 4 cut(s) 917, 1714, 3016, 4020
EcoT14I CCWWGG 2 cut(s) 244, 3516
EcoT22I ATGCAT 3 cut(s) 2272, 3358, 3601
EcoT38I GRGCYC 3 cut(s) 1100, 1487, 3975
EgeI GGCGCC 1 cut(s) 397
EheI GGCGCC 1 cut(s) 397
ErhI CCWWGG 2 cut(s) 244, 3516
Esp3I CGTCTC 2 cut(s) 187, 307
FaqI GGGAC 6 cut(s) 226, 920, 1508, 2536, 3722, 3914
FauI CCCGC 2 cut(s) 430, 1087
FauNDI CATATG 1 cut(s) 673
FbaI TGATCA 1 cut(s) 1546
FblI GTMKAC 1 cut(s) 150
FriOI GRGCYC 3 cut(s) 1100, 1487, 3975
FspBI CTAG 6 cut(s) 491, 603, 1469, 2330, 3146, 4037
GlaI GCGC 2 cut(s) 397, 2903
GsaI CCCAGC 1 cut(s) 1158
GsuI CTGGAG 3 cut(s) 2296, 2528, 3996
HaeII RGCGCY 2 cut(s) 399, 2905
HaeIII GGCC 7 cut(s) 249, 859, 871, 1175, 1792, 3123, 3914
HapII CCGG 4 cut(s) 168, 392, 459, 2659
HgaI GACGC 5 cut(s) 161, 386, 2522, 3382, 3823
HhaI GCGC 2 cut(s) 398, 2904
Hin1I GRCGYC 3 cut(s) 291, 378, 396
Hin6I GCGC 2 cut(s) 396, 2902
HinP1I GCGC 2 cut(s) 396, 2902
HincII GTYRAC 5 cut(s) 151, 1392, 1750, 3192, 3385
HindII GTYRAC 5 cut(s) 151, 1392, 1750, 3192, 3385
HindIII AAGCTT 3 cut(s) 2894, 3093, 3439
HpaI GTTAAC 2 cut(s) 1392, 1750
HpaII CCGG 4 cut(s) 168, 392, 459, 2659
HphI GGTGA 9 cut(s) 473, 860, 991, 1605, 1668, 1883, 1903, 2585, 4010
Hpy99I CGWCG 3 cut(s) 152, 155, 2219
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 152, 168, 337, 3457, 3542
HpyF3I CTNAG 7 cut(s) 1046, 1238, 1486, 1584, 3942, 3969, 4152
Hsp92I GRCGYC 3 cut(s) 291, 378, 396
HspAI GCGC 2 cut(s) 396, 2902
KasI GGCGCC 1 cut(s) 395
Kpn2I TCCGGA 1 cut(s) 2658
KpnI GGTACC 1 cut(s) 1992
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Ksp22I TGATCA 1 cut(s) 1546
KspAI GTTAAC 2 cut(s) 1392, 1750
Lsp1109I GCAGC 8 cut(s) 747, 2206, 2403, 2437, 2513, 2924, 3958, 4037
MaeI CTAG 6 cut(s) 491, 603, 1469, 2330, 3146, 4037
MbiI CCGCTC 2 cut(s) 275, 331
MfeI CAATTG 2 cut(s) 235, 3741
MflI RGATCY 6 cut(s) 1002, 1471, 2531, 2566, 3208, 3906
MhlI GDGCHC 6 cut(s) 1100, 1487, 2438, 2698, 3133, 3975
MlsI TGGCCA 4 cut(s) 859, 1175, 3123, 3914
MluI ACGCGT 1 cut(s) 153
MluNI TGGCCA 4 cut(s) 859, 1175, 3123, 3914
Mly113I GGCGCC 1 cut(s) 396
MlyI GAGTC 2 cut(s) 627, 3197
MmeI TCCRAC 4 cut(s) 601, 2136, 3259, 3348
Mox20I TGGCCA 4 cut(s) 859, 1175, 3123, 3914
Mph1103I ATGCAT 3 cut(s) 2272, 3358, 3601
MroI TCCGGA 1 cut(s) 2658
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 4 cut(s) 1416, 2846, 3825, 4143
MscI TGGCCA 4 cut(s) 859, 1175, 3123, 3914
MslI CAYNNNNRTG 2 cut(s) 459, 2463
Msp20I TGGCCA 4 cut(s) 859, 1175, 3123, 3914
MspA1I CMGCKG 4 cut(s) 1094, 2915, 3941, 4191
MspI CCGG 4 cut(s) 168, 392, 459, 2659
MspR9I CCNGG 6 cut(s) 392, 393, 919, 1716, 3018, 4022
MunI CAATTG 2 cut(s) 235, 3741
Mva1269I GAATGC 1 cut(s) 3301
MvaI CCWGG 4 cut(s) 919, 1716, 3018, 4022
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 6 cut(s) 44, 152, 168, 337, 3457, 3542
NaeI GCCGGC 1 cut(s) 169
NarI GGCGCC 1 cut(s) 396
NciI CCSGG 2 cut(s) 392, 393
NdeI CATATG 1 cut(s) 673
NgoMIV GCCGGC 1 cut(s) 167
NlaIV GGNNCC 7 cut(s) 242, 321, 397, 1099, 1990, 3015, 3157
NmeAIII GCCGAG 1 cut(s) 1432
NmuCI GTSAC 8 cut(s) 1450, 1510, 1601, 1909, 2232, 2494, 3250, 4090
NsiI ATGCAT 3 cut(s) 2272, 3358, 3601
NspI RCATGY 8 cut(s) 827, 1130, 2274, 2294, 2520, 2592, 3105, 3804
NspV TTCGAA 1 cut(s) 2776
OliI CACNNNNGTG 1 cut(s) 459
PaeI GCATGC 1 cut(s) 2274
PagI TCATGA 2 cut(s) 1543, 2970
PceI AGGCCT 1 cut(s) 249
PciI ACATGT 1 cut(s) 3101
PcsI WCGNNNNNNNCGW 2 cut(s) 297, 2782
PctI GAATGC 1 cut(s) 3301
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 4 cut(s) 1416, 2846, 3825, 4143
PfeI GAWTC 9 cut(s) 14, 584, 1084, 1370, 1967, 2719, 2988, 3048, 4068
PflMI CCANNNNNTGG 1 cut(s) 4052
PfoI TCCNGGA 1 cut(s) 3016
PinAI ACCGGT 1 cut(s) 458
Ple19I CGATCG 1 cut(s) 1528
PleI GAGTC 2 cut(s) 626, 3196
PluTI GGCGCC 1 cut(s) 399
PmaCI CACGTG 2 cut(s) 3335, 4052
PmlI CACGTG 2 cut(s) 3335, 4052
PpsI GAGTC 2 cut(s) 626, 3196
Ppu21I YACGTR 4 cut(s) 736, 2786, 3335, 4052
PscI ACATGT 1 cut(s) 3101
PshBI ATTAAT 2 cut(s) 1647, 3216
Psp124BI GAGCTC 2 cut(s) 1487, 3975
Psp1406I AACGTT 1 cut(s) 3365
Psp6I CCWGG 4 cut(s) 917, 1714, 3016, 4020
PspCI CACGTG 2 cut(s) 3335, 4052
PspFI CCCAGC 1 cut(s) 1154
PspGI CCWGG 4 cut(s) 917, 1714, 3016, 4020
PspN4I GGNNCC 7 cut(s) 242, 321, 397, 1099, 1990, 3015, 3157
PspPI GGNCC 4 cut(s) 22, 240, 1790, 3156
PstI CTGCAG 1 cut(s) 2455
PstNI CAGNNNCTG 4 cut(s) 1631, 2684, 2915, 4127
PsuI RGATCY 6 cut(s) 1002, 1471, 2531, 2566, 3208, 3906
PvuI CGATCG 1 cut(s) 1528
PvuII CAGCTG 3 cut(s) 2915, 3941, 4191
RseI CAYNNNNRTG 2 cut(s) 459, 2463
SacI GAGCTC 2 cut(s) 1487, 3975
SalI GTCGAC 1 cut(s) 149
Sau96I GGNCC 4 cut(s) 22, 240, 1790, 3156
SchI GAGTC 2 cut(s) 627, 3197
ScrFI CCNGG 6 cut(s) 392, 393, 919, 1716, 3018, 4022
SduI GDGCHC 6 cut(s) 1100, 1487, 2438, 2698, 3133, 3975
SfcI CTRYAG 7 cut(s) 1018, 1089, 1215, 2259, 2451, 2874, 2916
SfoI GGCGCC 1 cut(s) 397
SfuI TTCGAA 1 cut(s) 2776
SgrAI CRCCGGYG 1 cut(s) 458
SgrDI CGTCGACG 1 cut(s) 149
SinI GGWCC 3 cut(s) 22, 240, 3156
SmaI CCCGGG 1 cut(s) 393
SmiMI CAYNNNNRTG 2 cut(s) 459, 2463
SmlI CTYRAG 3 cut(s) 1192, 2583, 2826
SmoI CTYRAG 3 cut(s) 1192, 2583, 2826
SnaBI TACGTA 1 cut(s) 2786
SpeI ACTAGT 1 cut(s) 3145
SphI GCATGC 1 cut(s) 2274
SrfI GCCCGGGC 1 cut(s) 393
SseBI AGGCCT 1 cut(s) 249
SsiI CCGC 9 cut(s) 45, 160, 222, 273, 329, 423, 755, 1094, 3451
SspDI GGCGCC 1 cut(s) 395
SspI AATATT 3 cut(s) 1386, 1645, 1693
SspMI CTAG 6 cut(s) 491, 603, 1469, 2330, 3146, 4037
SstI GAGCTC 2 cut(s) 1487, 3975
StuI AGGCCT 1 cut(s) 249
StyD4I CCNGG 6 cut(s) 390, 391, 917, 1714, 3016, 4020
StyI CCWWGG 2 cut(s) 244, 3516
TaqI TCGA 8 cut(s) 150, 745, 1802, 2698, 2776, 3197, 3405, 4066
TatI WGTACW 5 cut(s) 1347, 2191, 2550, 2671, 2935
TauI GCSGC 1 cut(s) 331
TfiI GAWTC 9 cut(s) 14, 584, 1084, 1370, 1967, 2719, 2988, 3048, 4068
TseFI GTSAC 8 cut(s) 1450, 1510, 1601, 1909, 2232, 2494, 3250, 4090
TseI GCWGC 9 cut(s) 760, 2219, 2416, 2450, 2501, 2912, 3946, 4025, 4188
Tsp45I GTSAC 8 cut(s) 1450, 1510, 1601, 1909, 2232, 2494, 3250, 4090
TspGWI ACGGA 4 cut(s) 35, 169, 792, 812
TspMI CCCGGG 1 cut(s) 391
Van91I CCANNNNNTGG 1 cut(s) 4052
VneI GTGCAC 2 cut(s) 2434, 3129
VpaK11BI GGWCC 3 cut(s) 22, 240, 3156
VspI ATTAAT 2 cut(s) 1647, 3216
XapI RAATTY 9 cut(s) 560, 874, 1517, 1538, 1780, 2249, 2842, 3135, 3824
XceI RCATGY 8 cut(s) 827, 1130, 2274, 2294, 2520, 2592, 3105, 3804
XcmI CCANNNNNNNNNTGG 2 cut(s) 2950, 3747
XmaI CCCGGG 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 150
XmnI GAANNNNTTC 4 cut(s) 1416, 2846, 3825, 4143
XspI CTAG 6 cut(s) 491, 603, 1469, 2330, 3146, 4037
ZraI GACGTC 1 cut(s) 292
Zsp2I ATGCAT 3 cut(s) 2272, 3358, 3601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.