RLG00000024101

ribosome production factor 2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0015375
Physical Location & Seq
Forward (+)
31898312 .. 31900519
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024101

Sequence Viewer

Length: 984 bp
ATGTTGAGAGTGAAAACTCCCCAGAATGCCAGGGCCAGAAGAGCGCTCGAGAAGCGTGCCCCCAAGCTCATCGAGACCGGGAAGAAGACTCTGATACTCCACGGCATGAGAACCAGCGCCGTCTTGAACGCGGTGTTATCAGACATTTACCATTTAAAGAAGGAGAGCGCCGTGAAATTCAGCCGCCGGAATAAGGATGTCTGCCCTTTCGAAAATGGTGGTGAGACCTCGCTTGAGTTCTTTTCGCATCAAACCGATTGCAGTCTTTTTGTTTATGGTTCTCATACAAAGAAGCGGCCCAACAATCTTGTCCTAGGGAGAACATACGATCACCATATCTATGATCTTATAGAGGTTGGGGTTGACAACTATAAACGCATGGACTCATTTTCATATGATAAGAAACTATCTCCACATGTGGGGTCGAAGCCTTTCATTTGTTTTATTGGAGAAGGATTTGAGAGCGTAGAAGAGCTGAAACATTTGAAGGAGGTTTTACTTGATCTGTTGCGAGGAGAGGTTGTAGAGAAATTAAATCTTGCTGGGTTAGATCGTGTATATCTATGTACAGCTCTATCCGCAAACAAAGTGATCCTTACACAGTGTGCACTGCGGCTGAAAAAGTCTGGCACAGTAGTTCCAAGGACTGAACTGGTAGAAGTTGGACCCTCCATGGATTTGGTAGTTCGTCGGCATCGTCTTCCTAATGATGGCCTAAGGAAAGAAGCCATGAGAGTAGTTAGAGATCAGCCTAAGAAGGACAAAAATATTCAACAGGACACACTGCGAGGCAAGGTTGGCAAAATCAACATTCCAGATCAGAAGGTTGATGACACGAAGTATTTCCACACAAAGGTTGGAGATTCGTATGTGCCTCACAAGACAAAAGGAATTAAAAGGGAGCGCCGTGAAGCTAAAACGAGAAAGGAGGAAAAAGAGGGCGATGAGAATGCATCAAAAAGACAGAAGGAAGATACTGAGTAA

Protein Analysis

328

Amino Acids

37.44

Weight (kDa)

9.62

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 37 - 231 6.1e-38 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015375)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23620
fragaria_vesca FvH4_6g17900
malus_domestica MD12G1018000.v1.1 MD14G1016000.v1.1
prunus_persica Prupe.7G117100_v2.0.a1
pyrus_communis pycom12g01500 pycom14g01380
rosa_chinensis RchiOBHm_Chr3g0472151
rosa_laevigata RLG00000012848 RLG00000024101
rosa_multiflora Rmu_sc0002532.1_g000027
rosa_roxburghii Rroxscaffold_6G00409200
rosa_rugosa Rorug03G0122700
rosa_samantha Rh3AG174500 Rh3CG191000
rosa_wichuraiana Rw3G016640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 131
AciI CCGC 5 cut(s) 131, 184, 295, 579, 613
AclWI GGATC 1 cut(s) 586
AcsI RAATTY 1 cut(s) 176
AdeI CACNNNGTG 1 cut(s) 605
AfaI GTAC 1 cut(s) 568
AfeI AGCGCT 1 cut(s) 45
AfiI CCNNNNNNNGG 4 cut(s) 193, 419, 710, 853
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 3 cut(s) 127, 487, 773
AjnI CCWGG 1 cut(s) 29
AluBI AGCT 4 cut(s) 67, 475, 572, 914
AluI AGCT 4 cut(s) 67, 475, 572, 914
Alw21I GWGCWC 1 cut(s) 610
Alw26I GTCTC 2 cut(s) 68, 218
Alw44I GTGCAC 1 cut(s) 606
AlwI GGATC 1 cut(s) 586
Ama87I CYCGRG 1 cut(s) 47
Aor51HI AGCGCT 1 cut(s) 45
AoxI GGCC 3 cut(s) 33, 296, 712
ApaLI GTGCAC 1 cut(s) 606
ApoI RAATTY 1 cut(s) 176
Asp700I GAANNNNTTC 2 cut(s) 431, 842
AspA2I CCTAGG 1 cut(s) 313
AspLEI GCGC 4 cut(s) 46, 119, 170, 906
AspS9I GGNCC 3 cut(s) 33, 297, 665
AsuC2I CCSGG 1 cut(s) 79
AsuHPI GGTGA 2 cut(s) 233, 323
AsuII TTCGAA 1 cut(s) 210
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 1 cut(s) 665
AvrII CCTAGG 1 cut(s) 313
AxyI CCTNAGG 1 cut(s) 716
BaeGI GKGCMC 2 cut(s) 61, 610
BbsI GAAGAC 2 cut(s) 92, 692
Bbv12I GWGCWC 1 cut(s) 610
BccI CCATC 1 cut(s) 704
BceAI ACGGC 4 cut(s) 104, 118, 155, 891
BcgI CGANNNNNNTGC 4 cut(s) 38, 72, 932, 966
BciT130I CCWGG 1 cut(s) 31
BcnI CCSGG 1 cut(s) 79
BcoDI GTCTC 2 cut(s) 68, 218
BfaI CTAG 1 cut(s) 314
BfoI RGCGCY 4 cut(s) 47, 120, 171, 907
BisI GCNGC 3 cut(s) 184, 296, 614
BlnI CCTAGG 1 cut(s) 313
BlsI GCNGC 3 cut(s) 185, 297, 615
Bme1390I CCNGG 2 cut(s) 31, 79
Bme18I GGWCC 1 cut(s) 665
BmeT110I CYCGRG 1 cut(s) 47
BmgT120I GGNCC 3 cut(s) 33, 297, 665
BmiI GGNNCC 1 cut(s) 667
BmrFI CCNGG 2 cut(s) 31, 79
BmsI GCATC 3 cut(s) 256, 703, 962
BpiI GAAGAC 2 cut(s) 92, 692
Bpu14I TTCGAA 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 79
BsaI GGTCTC 2 cut(s) 68, 218
BsaJI CCNNGG 5 cut(s) 30, 100, 313, 641, 672
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 4 cut(s) 193, 419, 710, 853
Bse1I ACTGG 1 cut(s) 657
Bse21I CCTNAGG 1 cut(s) 716
BseBI CCWGG 1 cut(s) 31
BseDI CCNNGG 5 cut(s) 30, 100, 313, 641, 672
BseGI GGATG 1 cut(s) 202
BseLI CCNNNNNNNGG 4 cut(s) 193, 419, 710, 853
BseMII CTCAG 1 cut(s) 969
BseNI ACTGG 1 cut(s) 657
BseRI GAGGAG 1 cut(s) 528
BseSI GKGCMC 2 cut(s) 61, 610
BseYI CCCAGC 1 cut(s) 542
Bsh1236I CGCG 1 cut(s) 131
BshFI GGCC 3 cut(s) 35, 298, 714
BsiHKAI GWGCWC 1 cut(s) 610
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 2 cut(s) 78, 187
BslI CCNNNNNNNGG 4 cut(s) 193, 419, 710, 853
BsmAI GTCTC 2 cut(s) 68, 218
BsmI GAATGC 2 cut(s) 31, 955
BsnI GGCC 3 cut(s) 35, 298, 714
Bso31I GGTCTC 2 cut(s) 68, 218
BsoBI CYCGRG 1 cut(s) 47
Bsp119I TTCGAA 1 cut(s) 210
Bsp1286I GDGCHC 2 cut(s) 61, 610
Bsp1407I TGTACA 1 cut(s) 566
Bsp143I GATC 7 cut(s) 328, 343, 502, 550, 591, 745, 817
Bsp19I CCATGG 1 cut(s) 672
BspACI CCGC 5 cut(s) 131, 184, 295, 579, 613
BspANI GGCC 3 cut(s) 35, 298, 714
BspCNI CTCAG 1 cut(s) 970
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 1 cut(s) 667
BspPI GGATC 1 cut(s) 586
BspQI GCTCTTC 2 cut(s) 34, 465
BspT104I TTCGAA 1 cut(s) 210
BspTNI GGTCTC 2 cut(s) 68, 218
BsrGI TGTACA 1 cut(s) 566
BsrI ACTGG 1 cut(s) 657
BssECI CCNNGG 5 cut(s) 30, 100, 313, 641, 672
BssMI GATC 7 cut(s) 328, 343, 502, 550, 591, 745, 817
BssT1I CCWWGG 3 cut(s) 313, 641, 672
Bst2UI CCWGG 1 cut(s) 31
Bst4CI ACNGT 2 cut(s) 603, 634
Bst6I CTCTTC 2 cut(s) 34, 465
BstAUI TGTACA 1 cut(s) 566
BstBI TTCGAA 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 57
BstDEI CTNAG 3 cut(s) 716, 753, 978
BstDSI CCRYGG 2 cut(s) 100, 672
BstF5I GGATG 1 cut(s) 202
BstFNI CGCG 1 cut(s) 131
BstH2I RGCGCY 4 cut(s) 47, 120, 171, 907
BstHHI GCGC 4 cut(s) 46, 119, 170, 906
BstKTI GATC 7 cut(s) 331, 346, 505, 553, 594, 748, 820
BstMAI GTCTC 2 cut(s) 68, 218
BstMBI GATC 7 cut(s) 328, 343, 502, 550, 591, 745, 817
BstMWI GCNNNNNNNGC 4 cut(s) 41, 52, 578, 798
BstNI CCWGG 1 cut(s) 31
BstNSI RCATGY 1 cut(s) 419
BstSCI CCNGG 2 cut(s) 29, 77
BstSLI GKGCMC 2 cut(s) 61, 610
BstUI CGCG 1 cut(s) 131
BstV2I GAAGAC 2 cut(s) 92, 692
BstXI CCANNNNNNTGG 1 cut(s) 679
Bsu36I CCTNAGG 1 cut(s) 716
BsuRI GGCC 3 cut(s) 35, 298, 714
BtgI CCRYGG 2 cut(s) 100, 672
BtgZI GCGATG 1 cut(s) 957
BtsCI GGATG 1 cut(s) 202
BtsI GCAGTG 2 cut(s) 608, 782
BtsIMutI CAGTG 3 cut(s) 608, 608, 782
Cac8I GCNNGC 1 cut(s) 57
CfoI GCGC 4 cut(s) 46, 119, 170, 906
Cfr13I GGNCC 3 cut(s) 33, 297, 665
Csp6I GTAC 1 cut(s) 567
CviAII CATG 5 cut(s) 106, 379, 416, 673, 730
CviQI GTAC 1 cut(s) 567
DdeI CTNAG 3 cut(s) 716, 753, 978
DpnI GATC 7 cut(s) 330, 345, 504, 552, 593, 747, 819
DpnII GATC 7 cut(s) 328, 343, 502, 550, 591, 745, 817
DraI TTTAAA 1 cut(s) 156
DraIII CACNNNGTG 1 cut(s) 605
Eam1104I CTCTTC 2 cut(s) 34, 465
EarI CTCTTC 2 cut(s) 34, 465
Eco130I CCWWGG 3 cut(s) 313, 641, 672
Eco31I GGTCTC 2 cut(s) 68, 218
Eco47I GGWCC 1 cut(s) 665
Eco47III AGCGCT 1 cut(s) 45
Eco81I CCTNAGG 1 cut(s) 716
Eco88I CYCGRG 1 cut(s) 47
EcoRII CCWGG 1 cut(s) 29
EcoT14I CCWWGG 3 cut(s) 313, 641, 672
EcoT22I ATGCAT 1 cut(s) 955
ErhI CCWWGG 3 cut(s) 313, 641, 672
FaeI CATG 5 cut(s) 109, 382, 419, 676, 733
FalI AAGNNNNNCTT 2 cut(s) 579, 611
FatI CATG 5 cut(s) 105, 378, 415, 672, 729
FauNDI CATATG 1 cut(s) 394
Fnu4HI GCNGC 3 cut(s) 184, 296, 614
FokI GGATG 1 cut(s) 209
Fsp4HI GCNGC 3 cut(s) 184, 296, 614
FspBI CTAG 1 cut(s) 314
GlaI GCGC 4 cut(s) 45, 118, 169, 905
GluI GCNGC 3 cut(s) 184, 296, 614
GsaI CCCAGC 1 cut(s) 546
HaeII RGCGCY 4 cut(s) 47, 120, 171, 907
HaeIII GGCC 3 cut(s) 35, 298, 714
HapII CCGG 2 cut(s) 78, 187
HhaI GCGC 4 cut(s) 46, 119, 170, 906
Hin1II CATG 5 cut(s) 109, 382, 419, 676, 733
Hin6I GCGC 4 cut(s) 44, 117, 168, 904
HinP1I GCGC 4 cut(s) 44, 117, 168, 904
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 3 cut(s) 88, 383, 863
HpaII CCGG 2 cut(s) 78, 187
HphI GGTGA 2 cut(s) 233, 323
Hpy166II GTNNAC 2 cut(s) 364, 608
Hpy188I TCNGA 3 cut(s) 93, 142, 822
Hpy188III TCNNGA 4 cut(s) 49, 73, 124, 815
Hpy8I GTNNAC 2 cut(s) 364, 608
Hpy99I CGWCG 1 cut(s) 693
HpyAV CCTTC 6 cut(s) 154, 446, 481, 751, 817, 961
HpyCH4III ACNGT 2 cut(s) 603, 634
HpyCH4V TGCA 3 cut(s) 261, 608, 953
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 52, 578, 798
HpyF3I CTNAG 3 cut(s) 716, 753, 978
Hsp92II CATG 5 cut(s) 109, 382, 419, 676, 733
HspAI GCGC 4 cut(s) 44, 117, 168, 904
Kzo9I GATC 7 cut(s) 328, 343, 502, 550, 591, 745, 817
LguI GCTCTTC 2 cut(s) 34, 465
LmnI GCTCC 1 cut(s) 901
LweI GCATC 3 cut(s) 256, 703, 962
MaeI CTAG 1 cut(s) 314
MalI GATC 7 cut(s) 330, 345, 504, 552, 593, 747, 819
MboI GATC 7 cut(s) 328, 343, 502, 550, 591, 745, 817
MboII GAAGA 6 cut(s) 51, 94, 97, 482, 692, 983
MhlI GDGCHC 2 cut(s) 61, 610
MluCI AATT 3 cut(s) 176, 530, 891
MlyI GAGTC 2 cut(s) 82, 377
MmeI TCCRAC 2 cut(s) 643, 838
Mph1103I ATGCAT 1 cut(s) 955
MroXI GAANNNNTTC 2 cut(s) 431, 842
MseI TTAA 3 cut(s) 155, 533, 894
MslI CAYNNNNRTG 1 cut(s) 339
MspI CCGG 2 cut(s) 78, 187
MspR9I CCNGG 2 cut(s) 31, 79
Mva1269I GAATGC 2 cut(s) 31, 955
MvaI CCWGG 1 cut(s) 31
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 4 cut(s) 41, 52, 578, 798
NciI CCSGG 1 cut(s) 79
NcoI CCATGG 1 cut(s) 672
NdeI CATATG 1 cut(s) 394
NdeII GATC 7 cut(s) 328, 343, 502, 550, 591, 745, 817
NlaIII CATG 5 cut(s) 109, 382, 419, 676, 733
NlaIV GGNNCC 1 cut(s) 667
NsiI ATGCAT 1 cut(s) 955
NspI RCATGY 1 cut(s) 419
NspV TTCGAA 1 cut(s) 210
PaeR7I CTCGAG 1 cut(s) 47
PciI ACATGT 1 cut(s) 415
PciSI GCTCTTC 2 cut(s) 34, 465
PcsI WCGNNNNNNNCGW 1 cut(s) 694
PctI GAATGC 2 cut(s) 31, 955
PdmI GAANNNNTTC 2 cut(s) 431, 842
PfeI GAWTC 1 cut(s) 863
PkrI GCNGC 3 cut(s) 185, 297, 615
PleI GAGTC 2 cut(s) 82, 377
PpsI GAGTC 2 cut(s) 82, 377
PscI ACATGT 1 cut(s) 415
Psp6I CCWGG 1 cut(s) 29
PspFI CCCAGC 1 cut(s) 542
PspGI CCWGG 1 cut(s) 29
PspN4I GGNNCC 1 cut(s) 667
PspPI GGNCC 3 cut(s) 33, 297, 665
RsaI GTAC 1 cut(s) 568
RsaNI GTAC 1 cut(s) 567
RseI CAYNNNNRTG 1 cut(s) 339
SapI GCTCTTC 2 cut(s) 34, 465
SaqAI TTAA 3 cut(s) 155, 533, 894
SatI GCNGC 3 cut(s) 184, 296, 614
Sau3AI GATC 7 cut(s) 328, 343, 502, 550, 591, 745, 817
Sau96I GGNCC 3 cut(s) 33, 297, 665
SchI GAGTC 2 cut(s) 82, 377
ScrFI CCNGG 2 cut(s) 31, 79
SduI GDGCHC 2 cut(s) 61, 610
SfaNI GCATC 3 cut(s) 256, 703, 962
Sfr274I CTCGAG 1 cut(s) 47
SfuI TTCGAA 1 cut(s) 210
SinI GGWCC 1 cut(s) 665
SlaI CTCGAG 1 cut(s) 47
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 2 cut(s) 47, 233
SmoI CTYRAG 2 cut(s) 47, 233
Sse9I AATT 3 cut(s) 176, 530, 891
SsiI CCGC 5 cut(s) 131, 184, 295, 579, 613
SspI AATATT 1 cut(s) 769
SspMI CTAG 1 cut(s) 314
StyD4I CCNGG 2 cut(s) 29, 77
StyI CCWWGG 3 cut(s) 313, 641, 672
TaaI ACNGT 2 cut(s) 603, 634
TaqI TCGA 4 cut(s) 48, 72, 210, 425
TasI AATT 3 cut(s) 176, 530, 891
TatI WGTACW 1 cut(s) 566
TauI GCSGC 3 cut(s) 186, 298, 616
TfiI GAWTC 1 cut(s) 863
Tru1I TTAA 3 cut(s) 155, 533, 894
Tru9I TTAA 3 cut(s) 155, 533, 894
TscAI CASTG 3 cut(s) 608, 615, 789
TspDTI ATGAA 2 cut(s) 381, 424
TspRI CASTG 3 cut(s) 608, 615, 789
VneI GTGCAC 1 cut(s) 606
VpaK11BI GGWCC 1 cut(s) 665
XapI RAATTY 1 cut(s) 176
XceI RCATGY 1 cut(s) 419
XcmI CCANNNNNNNNNTGG 1 cut(s) 854
XhoI CTCGAG 1 cut(s) 47
XmaJI CCTAGG 1 cut(s) 313
XmnI GAANNNNTTC 2 cut(s) 431, 842
XspI CTAG 1 cut(s) 314
Zsp2I ATGCAT 1 cut(s) 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.