RLG00000026136

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003922
Physical Location & Seq
Forward (+)
754480 .. 764020
9541 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026136

Sequence Viewer

Length: 4014 bp
ATGGCTGACATGCAAATCGTTCTGGCTTTAAAAACTAGTGTGGAAGCGCAGTATGTGGAAATGATGGTTCCTCTTTATTCTCCTGGGTGCGAGAAGAAAGTCAAGAAGGCCCTATCCCACCTCAAAGGAATATACTCGGTGAATGTAGACTGCAGCCAACAAAAGGTGACAGTTTGGGGAATATGCAACAAATACGATGTGCTAGCCACAGTAAGGACCAAGAGAAAACACGCTTGCTTTTGGAATCCTGAAGACAACGATATTGAAGAGGACGGAGAGGAAGAAGCAGAGGTACCACTGACCGTGACGATGTCGGAGTCAGACGACTCAACACCACCGATTGCAAAAAGAAAGTACTTATTAACACAGCCTTCTTTAAAGGCTCTTACCAGGGTTCGCTCTTTGAGCTTGAGAGCATGGAAGAAGGTCTTCACCAGACCCAGAGGACACTGCCACCACGGACTCTCTCTCTCTCTCTCTCCGTCTCTCCTCACTGTTTTGAACCAAAATTTAAGAATGATGGGATTTGGGACTTATGGATATGGAGGACCCTATTTGTCATCATCATCATCTTCGAGTTTATCAGCTCTGGCACCACCTTTTACGGTTGAGCGGCCTGTTCCTAAGCCCACTTCGAGCCCACTGGTGGAGACCTTTACACCACTTGTGGAGGTGACTGAGCCACCTTATGCCGCCCCTATGAATTCTTCTTTGCACAACTGGCTCCCCCCTCACTCTCCCACTTCAGGGTCCAATTTCTTCGCCAACCCCACCCCATCATTTGATTCAGTGCCTTCATCCAATGCCTATAGATATGCAGGCTTGCCGGCTGTTGACTCATTCAGTACAAATCTGCCTCCGATGAACACTGTTACCACACCTTCGTCCAATGCATTTACTTATGATCAAGGCTTGGATGTGACAGCAACTAGCTTTGTGGAAGCGAAACCCTATTATCCCTCTTATTTATCGCCCACAATTCATGGTGATAGTCCTATGGTGGCTCCTGATCAACCTAGTTATGATTGGCTATCTACTTCTCAATTTGCTCCCTTGGATGGGTCGTCTCACAAAGAGTACACTCAACGGTCCTCTTCTTCAAAGTATACAGCTCAGTGGGGTGGCTCATGGAATGGACCAGCAGAGTGGGAGCAGGGTAAACAGGGACAATTTGATGGAAGTTTCGGCCCAAAGGAGACTGATGTATCTAGTTTTCCATATAAGAATTACCTGAACCAAGAACCTCATTCTTCCAATAGCTTGAAATCATACGGTATAAATGAAGTAGCATCCTGCAATATCCCAGATTGGGATGGATCTGTAAATGCAGAGCATTTAGGTGATAAATCCTTTGTGGGGAGAAATTCCAAATTCAGCCCCATTGACTTTACAAAACCGACTATCGGATCCCTTTCAGGGGTTCCAGACACCCCTTCTAAGGCACCATCTTCACCTTTTGTTGCGAAGTCCACATTTGGTGTCTCATGTGAGAAAAGACAGCATGATGCTAGTTGGAATGATATCACACCTATTTCAAAGTCTTCCCCTGCCTTTATCATCAGACCACCGGCTATAGGCACAAAATCTTCAGAACCAGAGATAGGTCTATTCAAAAGATTGAATTCCGGAAGAGATGCAGCCAATGCAGATCATGGTGGTTATATTCCCTCACTGGAGTCTCATCTCCCACGAAGATTTGAAGACAAAGTTCCTTTTGATTCAAGCCAACTCGCCATTCACCTAGGAAGGAATGATCCCTTCTCTGTGGAATCTTCATCAACAAAAGACAAAGAGCTGTCAAATAATGGAAGTATCAACAATGATGCCTTGGACCATTTGTTTAAAGTAAAACCTGGACTTCCAAATTCTCATGTTAATCCTGATGGTTTTGTTGCGGCACTTAATATCAATGATTCCATAAATTCTTTTCTGAATTCCTCTGAGAATGTGGATCCCAACAATCCAGCTGTGGACTCACCTTGTTGGAAGGGAGTTCCTGGTAGTCGTTTTTCTCCATTTAAAGCAACTGAAGAGGGAGGTCGAGAACAGATAAAGAAACTAGAGGGCTGCAGTGGCTTGAATCTTAAGATGCCAATGATCTTCTCACTAAACACAGGTGAAAATATCTCCACCCAAAAGCCGGTTGAGTACCATGAATTTGGATGGCTGGGGAATGATTTGCTCGGAAATGGTTTGCCACTTCATTTGAAGAAATCTTCGGTGGCAAATTCTGCTTTTGGAGAACATAAATTAGATGATACCATGAAGACTACCTGTTACCCTGAATCAAGCCATGATAGAGGGCTTCAAGGTTACATTAATACCCCTAATTCAGGGAGTGGAGACAAGTCCTCAAGTCTCTTTGAGCATTCTCACACTGTGCAACAAGGTCACGGAGAAGGTGGATTAACAACTGAAGCAAAGAACGCAAAATGGAGTGTTGGTGCAGATGTTGAATTGAATGTCAATGACACCTTGGAATGTGGTTCATCTCATACCTCTCCTGCTGTCGAAAATATATTTTGTTCTCCTTCTGTAGAGGATGCTGCTACAAAGCTCACCAAATCAAATGGGGAAGAATCAAATATGAATATGGATGTCCAGATGCTGGTTAATACAATGAATAGCTTGTCAGAATTGCTGCTTGTTAATTGTTCAAACAGTTCATGTCAGTTGAAGAAAAAAGATCTTGACTCCCTAAAAGCTGTAATCAATAACTTGAATAGCTGCATATCGAAGCATGATGAAAACTTGTTGTCAACGCAAGAATCAACCTCTTTCCAGCAAAGTACTCTCAAGTATATTGAAGAGCAAGAATCACCCCCTCTCCAGCAAAGTACTCTAACGTATATAGAAGAGTTATGCAAGCCAAATAAGGTTTTGAGTCCAGACAGGCCGCAGTTGACCAAAATATTTGCTCCTAGCATTCAGGATCCACTTCATCTTGAGGGTGTTCAGAAGGTAAAGAAGTATGATAGTTTTGTTAAGAATGATAATGAAGTTATAGGTTCTGTTTCTGCAAAAAGTGACATCGACTTGCTGAAACAAGATGAGATGACTCAGGCTGTAAAGAAGATTCTCAGTGAGAATTTCCATACCGAGGATACACACCCTCAGACTCTCTTGTACAAGAATCTATGGCTTGAAGCTGAAGCTGTAATATGTTCCACCAATTACAAGGCTCGTTTTAATCGTCTGAAAAATGAAATGGAGAAGTGCAAGGCTGACCAATCCAAAGATGTGTTTGAACACACTGCTGATATGATGACGCAATCAAGGTCTGAGGTTTACCCTAATTCACGCTCAGTTGAGAAGTTGACACCTGAAGTTCAGGGTTGTCCAAAGTTTAAACTCCAGGAGTCTCCTACTCTAAACCGAGAAGATGAAGATGTTATGGCAAGATTTCATATCTTAAGGAACCGCATTGAGGACTCACATTCCACAAATGCTACCAATGGGGATGAATCCAGCTCAAGTCTTTCTCCTGTTCCCGACAAGGTCGGCGAGGTTGCACCTGATGCAGATGCTAGGCTCTCACCTACGATCTCCATCCAGGACTCTCCCACATCAAGCATAATTGGCCGGACAAATGATTATGAGGCTTCTGTTATGGCCAGATTTCATATTTTAAGAGACCGGGTCGAGAACTCAAAATTCATTAGTGATGCCAATGTGGAGGATACAGTGAGCTCCAAGGTCTCTCATGAACACAAGGCTGAGGAAGTTGCACCTGTAACAAGTGATGATGGCTCAATTCAGGAGTTTAGCATTCAGGATTACCTTGTTTCGAGCACAACTGGCCATGCAAATCAGTATGATGATGGCTCAATTCAGGAGTTAAATATTCAGGATTACCTTGATTCAACCAGAACAGGCCATGCATTTCAGTACGAGGATTCCGTTTTGGCCAGATTTAATATCCTTAAATCTCGGGTTGACAACTGTAGTGACATGCCTACAGTAGAGCAACCTCTGGAAAATGTCGATCTTGGATATGCTGAGTTACATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1338

Amino Acids

147.01

Weight (kDa)

5.27

Isoelectric Point (pI)

50.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 24 - 59 1.8e-06 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 292
AccB1I GGYRCC 3 cut(s) 292, 592, 1441
AccB7I CCANNNNNTGG 1 cut(s) 2608
AccBSI CCGCTC 1 cut(s) 613
AccI GTMKAC 2 cut(s) 147, 1106
AccIII TCCGGA 1 cut(s) 1625
AciI CCGC 5 cut(s) 613, 693, 1895, 2897, 3421
AclWI GGATC 8 cut(s) 1324, 1401, 1414, 1748, 1946, 1959, 2927, 2940
AcoI YGGCCR 4 cut(s) 3580, 3612, 3799, 3906
AcuI CTGAAG 7 cut(s) 270, 729, 1572, 2049, 2436, 3171, 3345
AdeI CACNNNGTG 1 cut(s) 2380
AfaI GTAC 9 cut(s) 294, 356, 847, 1079, 2150, 2791, 2839, 3128, 3890
AflII CTTAAG 2 cut(s) 2084, 3412
AhlI ACTAGT 1 cut(s) 35
AjnI CCWGG 6 cut(s) 82, 389, 1852, 1996, 3354, 3552
AjuI GAANNNNNNNTTGG 2 cut(s) 1719, 1751
Alw21I GWGCWC 2 cut(s) 3692, 3794
AlwI GGATC 8 cut(s) 1324, 1401, 1414, 1748, 1946, 1959, 2927, 2940
AlwNI CAGNNNCTG 1 cut(s) 2608
Ama87I CYCGRG 1 cut(s) 3930
Aor13HI TCCGGA 1 cut(s) 1625
AoxI GGCC 9 cut(s) 108, 614, 1186, 2894, 3580, 3612, 3799, 3874, 3906
ApeKI GCWGC 6 cut(s) 153, 1637, 2067, 2546, 2641, 2727
AseI ATTAAT 1 cut(s) 2319
Asp700I GAANNNNTTC 1 cut(s) 428
Asp718I GGTACC 1 cut(s) 292
AspA2I CCTAGG 1 cut(s) 1741
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 8 cut(s) 109, 216, 548, 750, 1089, 1136, 1187, 1831
AsuC2I CCSGG 1 cut(s) 3638
AsuNHI GCTAGC 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 3930
AvaII GGWCC 6 cut(s) 216, 548, 750, 1089, 1136, 1831
AvrII CCTAGG 1 cut(s) 1741
BaeI ACNNNNGTAYC 2 cut(s) 1188, 1221
BalI TGGCCA 3 cut(s) 3614, 3801, 3908
BamHI GGATCC 3 cut(s) 1406, 1951, 2932
BanI GGYRCC 3 cut(s) 292, 592, 1441
BanII GRGCYC 2 cut(s) 641, 3692
BarI GAAGNNNNNNTAC 2 cut(s) 276, 308
BbsI GAAGAC 5 cut(s) 258, 421, 1533, 1707, 2273
Bbv12I GWGCWC 2 cut(s) 3692, 3794
BbvCI CCTCAGC 1 cut(s) 3717
BbvI GCAGC 6 cut(s) 165, 1649, 2054, 2533, 2628, 2714
BciT130I CCWGG 6 cut(s) 84, 391, 1854, 1998, 3356, 3554
BciVI GTATCC 2 cut(s) 3097, 3673
BclI TGATCA 2 cut(s) 904, 1009
BcnI CCSGG 1 cut(s) 3638
BcuI ACTAGT 1 cut(s) 35
BfmI CTRYAG 7 cut(s) 151, 808, 1572, 2068, 2535, 3943, 3957
BfrI CTTAAG 2 cut(s) 2084, 3412
BfuI GTATCC 2 cut(s) 3097, 3673
BglII AGATCT 1 cut(s) 2686
BlnI CCTAGG 1 cut(s) 1741
BmcAI AGTACT 3 cut(s) 356, 2791, 2839
Bme1390I CCNGG 7 cut(s) 84, 391, 1854, 1998, 3356, 3554, 3638
Bme18I GGWCC 6 cut(s) 216, 548, 750, 1089, 1136, 1831
BmeT110I CYCGRG 1 cut(s) 3930
BmgT120I GGNCC 8 cut(s) 109, 216, 548, 750, 1089, 1136, 1187, 1831
BmrFI CCNGG 7 cut(s) 84, 391, 1854, 1998, 3356, 3554, 3638
BmtI GCTAGC 1 cut(s) 206
BpiI GAAGAC 5 cut(s) 258, 421, 1533, 1707, 2273
BpmI CTGGAG 3 cut(s) 1694, 2813, 3338
Bpu10I CCTNAGC 2 cut(s) 624, 3717
BpuEI CTTGAG 5 cut(s) 430, 2338, 2780, 2966, 3457
BpuMI CCSGG 1 cut(s) 3638
BsaBI GATNNNNATC 1 cut(s) 3548
BsaI GGTCTC 3 cut(s) 644, 3627, 3703
BsaJI CCNNGG 9 cut(s) 83, 390, 457, 1053, 1741, 1827, 2475, 3099, 3693
BsaWI WCCGGW 1 cut(s) 1625
BsaXI ACNNNNNCTCC 4 cut(s) 2428, 2458, 2811, 2841
Bse118I RCCGGY 3 cut(s) 826, 1567, 2140
Bse1I ACTGG 4 cut(s) 648, 725, 1677, 3802
Bse8I GATNNNNATC 1 cut(s) 3548
BseAI TCCGGA 1 cut(s) 1625
BseBI CCWGG 6 cut(s) 84, 391, 1854, 1998, 3356, 3554
BseDI CCNNGG 9 cut(s) 83, 390, 457, 1053, 1741, 1827, 2475, 3099, 3693
BseJI GATNNNNATC 1 cut(s) 3548
BseNI ACTGG 4 cut(s) 648, 725, 1677, 3802
BseRI GAGGAG 1 cut(s) 479
BseXI GCAGC 6 cut(s) 165, 1649, 2054, 2533, 2628, 2714
BseYI CCCAGC 1 cut(s) 2167
BsgI GTGCAG 1 cut(s) 2466
BshFI GGCC 9 cut(s) 110, 616, 1188, 2896, 3582, 3614, 3801, 3876, 3908
BshNI GGYRCC 3 cut(s) 292, 592, 1441
BsiHKAI GWGCWC 2 cut(s) 3692, 3794
BsiHKCI CYCGRG 1 cut(s) 3930
BsiSI CCGG 6 cut(s) 827, 1568, 1626, 2141, 3583, 3637
BslFI GGGAC 2 cut(s) 544, 1179
BsmBI CGTCTC 2 cut(s) 489, 1071
BsmFI GGGAC 2 cut(s) 544, 1179
BsmI GAATGC 3 cut(s) 2368, 2925, 3768
BsnI GGCC 9 cut(s) 110, 616, 1188, 2896, 3582, 3614, 3801, 3876, 3908
Bso31I GGTCTC 3 cut(s) 644, 3627, 3703
BsoBI CYCGRG 1 cut(s) 3930
Bsp1286I GDGCHC 3 cut(s) 641, 3692, 3794
Bsp13I TCCGGA 1 cut(s) 1625
Bsp1407I TGTACA 1 cut(s) 3126
BspACI CCGC 5 cut(s) 613, 693, 1895, 2897, 3421
BspANI GGCC 9 cut(s) 110, 616, 1188, 2896, 3582, 3614, 3801, 3876, 3908
BspEI TCCGGA 1 cut(s) 1625
BspHI TCATGA 1 cut(s) 3703
BspMAI CTGCAG 2 cut(s) 155, 2072
BspOI GCTAGC 1 cut(s) 206
BspPI GGATC 8 cut(s) 1324, 1401, 1414, 1748, 1946, 1959, 2927, 2940
BspQI GCTCTTC 1 cut(s) 2802
BspT107I GGYRCC 3 cut(s) 292, 592, 1441
BspTI CTTAAG 2 cut(s) 2084, 3412
BspTNI GGTCTC 3 cut(s) 644, 3627, 3703
BsrBI CCGCTC 1 cut(s) 613
BsrFI RCCGGY 3 cut(s) 826, 1567, 2140
BsrGI TGTACA 1 cut(s) 3126
BsrI ACTGG 4 cut(s) 648, 725, 1677, 3802
BssAI RCCGGY 3 cut(s) 826, 1567, 2140
BssECI CCNNGG 9 cut(s) 83, 390, 457, 1053, 1741, 1827, 2475, 3099, 3693
BssNAI GTATAC 1 cut(s) 1107
BssT1I CCWWGG 5 cut(s) 1053, 1741, 1827, 2475, 3693
Bst1107I GTATAC 1 cut(s) 1107
Bst2UI CCWGG 6 cut(s) 84, 391, 1854, 1998, 3356, 3554
Bst6I CTCTTC 6 cut(s) 261, 1099, 1624, 2025, 2802, 2850
BstAFI CTTAAG 2 cut(s) 2084, 3412
BstAPI GCANNNNNTGC 3 cut(s) 1643, 2231, 3518
BstAUI TGTACA 1 cut(s) 3126
BstC8I GCNNGC 6 cut(s) 204, 235, 820, 824, 828, 2867
BstDSI CCRYGG 1 cut(s) 457
BstENI CCTNNNNNAGG 1 cut(s) 2331
BstHHI GCGC 1 cut(s) 49
BstMWI GCNNNNNNNGC 9 cut(s) 405, 721, 1643, 2073, 2231, 2426, 3518, 3579, 3798
BstNI CCWGG 6 cut(s) 84, 391, 1854, 1998, 3356, 3554
BstNSI RCATGY 2 cut(s) 13, 3955
BstSCI CCNGG 7 cut(s) 82, 389, 1852, 1996, 3354, 3552, 3636
BstSFI CTRYAG 7 cut(s) 151, 808, 1572, 2068, 2535, 3943, 3957
BstV1I GCAGC 6 cut(s) 165, 1649, 2054, 2533, 2628, 2714
BstV2I GAAGAC 5 cut(s) 258, 421, 1533, 1707, 2273
BstX2I RGATCY 5 cut(s) 1316, 1406, 1951, 2686, 2932
BstXI CCANNNNNNTGG 2 cut(s) 1146, 2159
BstYI RGATCY 5 cut(s) 1316, 1406, 1951, 2686, 2932
BstZ17I GTATAC 1 cut(s) 1107
BsuI GTATCC 2 cut(s) 3097, 3673
BsuRI GGCC 9 cut(s) 110, 616, 1188, 2896, 3582, 3614, 3801, 3876, 3908
BtgI CCRYGG 1 cut(s) 457
BtsI GCAGTG 3 cut(s) 448, 2077, 3252
Cac8I GCNNGC 6 cut(s) 204, 235, 820, 824, 828, 2867
CaiI CAGNNNCTG 1 cut(s) 2608
CciI TCATGA 1 cut(s) 3703
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 3 cut(s) 826, 1567, 2140
Cfr13I GGNCC 8 cut(s) 109, 216, 548, 750, 1089, 1136, 1187, 1831
CseI GACGC 1 cut(s) 3277
Csp6I GTAC 9 cut(s) 293, 355, 846, 1078, 2149, 2790, 2838, 3127, 3889
CspCI CAANNNNNGTGG 2 cut(s) 2187, 2222
CviQI GTAC 9 cut(s) 293, 355, 846, 1078, 2149, 2790, 2838, 3127, 3889
DraI TTTAAA 5 cut(s) 30, 378, 1843, 2020, 3349
DraIII CACNNNGTG 1 cut(s) 2380
EaeI YGGCCR 4 cut(s) 3580, 3612, 3799, 3906
Eam1104I CTCTTC 6 cut(s) 261, 1099, 1624, 2025, 2802, 2850
EarI CTCTTC 6 cut(s) 261, 1099, 1624, 2025, 2802, 2850
Ecl136II GAGCTC 1 cut(s) 3690
Eco130I CCWWGG 5 cut(s) 1053, 1741, 1827, 2475, 3693
Eco24I GRGCYC 2 cut(s) 641, 3692
Eco31I GGTCTC 3 cut(s) 644, 3627, 3703
Eco32I GATATC 1 cut(s) 1522
Eco47I GGWCC 6 cut(s) 216, 548, 750, 1089, 1136, 1831
Eco53kI GAGCTC 1 cut(s) 3690
Eco57I CTGAAG 7 cut(s) 270, 729, 1572, 2049, 2436, 3171, 3345
Eco88I CYCGRG 1 cut(s) 3930
EcoICRI GAGCTC 1 cut(s) 3690
EcoNI CCTNNNNNAGG 1 cut(s) 2331
EcoO109I RGGNCCY 2 cut(s) 109, 548
EcoRI GAATTC 3 cut(s) 703, 1621, 1933
EcoRII CCWGG 6 cut(s) 82, 389, 1852, 1996, 3354, 3552
EcoRV GATATC 1 cut(s) 1522
EcoT14I CCWWGG 5 cut(s) 1053, 1741, 1827, 2475, 3693
EcoT22I ATGCAT 2 cut(s) 895, 3883
EcoT38I GRGCYC 2 cut(s) 641, 3692
ErhI CCWWGG 5 cut(s) 1053, 1741, 1827, 2475, 3693
Esp3I CGTCTC 2 cut(s) 489, 1071
FalI AAGNNNNNCTT 2 cut(s) 413, 445
FaqI GGGAC 2 cut(s) 544, 1179
FbaI TGATCA 2 cut(s) 904, 1009
FblI GTMKAC 2 cut(s) 147, 1106
FriOI GRGCYC 2 cut(s) 641, 3692
GlaI GCGC 1 cut(s) 48
GsaI CCCAGC 1 cut(s) 2171
GsuI CTGGAG 3 cut(s) 1694, 2813, 3338
HaeIII GGCC 9 cut(s) 110, 616, 1188, 2896, 3582, 3614, 3801, 3876, 3908
HapII CCGG 6 cut(s) 827, 1568, 1626, 2141, 3583, 3637
HgaI GACGC 1 cut(s) 3277
HhaI GCGC 1 cut(s) 49
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HincII GTYRAC 5 cut(s) 835, 2760, 2904, 3318, 3937
HindII GTYRAC 5 cut(s) 835, 2760, 2904, 3318, 3937
HpaII CCGG 6 cut(s) 827, 1568, 1626, 2141, 3583, 3637
HpyCH4IV ACGT 1 cut(s) 2846
HpyF10VI GCNNNNNNNGC 9 cut(s) 405, 721, 1643, 2073, 2231, 2426, 3518, 3579, 3798
HpySE526I ACGT 1 cut(s) 2846
HspAI GCGC 1 cut(s) 47
Kpn2I TCCGGA 1 cut(s) 1625
KpnI GGTACC 1 cut(s) 296
KroI GCCGGC 1 cut(s) 826
KroNI GCCGGC 1 cut(s) 828
Ksp22I TGATCA 2 cut(s) 904, 1009
LguI GCTCTTC 1 cut(s) 2802
LmnI GCTCC 6 cut(s) 729, 1009, 1054, 1150, 2923, 3695
Lsp1109I GCAGC 6 cut(s) 165, 1649, 2054, 2533, 2628, 2714
MaeII ACGT 1 cut(s) 2846
MbiI CCGCTC 1 cut(s) 613
MflI RGATCY 5 cut(s) 1316, 1406, 1951, 2686, 2932
MhlI GDGCHC 3 cut(s) 641, 3692, 3794
MlsI TGGCCA 3 cut(s) 3614, 3801, 3908
MluNI TGGCCA 3 cut(s) 3614, 3801, 3908
MmeI TCCRAC 3 cut(s) 294, 1493, 1964
Mox20I TGGCCA 3 cut(s) 3614, 3801, 3908
Mph1103I ATGCAT 2 cut(s) 895, 3883
MroI TCCGGA 1 cut(s) 1625
MroNI GCCGGC 1 cut(s) 826
MroXI GAANNNNTTC 1 cut(s) 428
MscI TGGCCA 3 cut(s) 3614, 3801, 3908
MslI CAYNNNNRTG 1 cut(s) 1338
Msp20I TGGCCA 3 cut(s) 3614, 3801, 3908
MspA1I CMGCKG 1 cut(s) 1967
MspCI CTTAAG 2 cut(s) 2084, 3412
MspI CCGG 6 cut(s) 827, 1568, 1626, 2141, 3583, 3637
MspR9I CCNGG 7 cut(s) 84, 391, 1854, 1998, 3356, 3554, 3638
MssI GTTTAAAC 1 cut(s) 3349
Mva1269I GAATGC 3 cut(s) 2368, 2925, 3768
MvaI CCWGG 6 cut(s) 84, 391, 1854, 1998, 3356, 3554
MwoI GCNNNNNNNGC 9 cut(s) 405, 721, 1643, 2073, 2231, 2426, 3518, 3579, 3798
NaeI GCCGGC 1 cut(s) 828
NciI CCSGG 1 cut(s) 3638
NgoMIV GCCGGC 1 cut(s) 826
NheI GCTAGC 1 cut(s) 202
NmuCI GTSAC 7 cut(s) 166, 304, 673, 919, 2390, 3026, 3947
NsiI ATGCAT 2 cut(s) 895, 3883
NspI RCATGY 2 cut(s) 13, 3955
PagI TCATGA 1 cut(s) 3703
PciSI GCTCTTC 1 cut(s) 2802
PcsI WCGNNNNNNNCGW 2 cut(s) 3190, 3897
PctI GAATGC 3 cut(s) 2368, 2925, 3768
PdiI GCCGGC 1 cut(s) 828
PdmI GAANNNNTTC 1 cut(s) 428
PflFI GACNNNGTC 3 cut(s) 310, 3497, 3638
PflMI CCANNNNNTGG 1 cut(s) 2608
PfoI TCCNGGA 2 cut(s) 3354, 3552
PmeI GTTTAAAC 1 cut(s) 3349
PpuMI RGGWCCY 1 cut(s) 548
PshBI ATTAAT 1 cut(s) 2319
Psp124BI GAGCTC 1 cut(s) 3692
Psp5II RGGWCCY 1 cut(s) 548
Psp6I CCWGG 6 cut(s) 82, 389, 1852, 1996, 3354, 3552
PspFI CCCAGC 1 cut(s) 2167
PspGI CCWGG 6 cut(s) 82, 389, 1852, 1996, 3354, 3552
PspPI GGNCC 8 cut(s) 109, 216, 548, 750, 1089, 1136, 1187, 1831
PspPPI RGGWCCY 1 cut(s) 548
PstI CTGCAG 2 cut(s) 155, 2072
PstNI CAGNNNCTG 1 cut(s) 2608
PsuI RGATCY 5 cut(s) 1316, 1406, 1951, 2686, 2932
PsyI GACNNNGTC 3 cut(s) 310, 3497, 3638
PvuII CAGCTG 1 cut(s) 1967
RsaI GTAC 9 cut(s) 294, 356, 847, 1079, 2150, 2791, 2839, 3128, 3890
RsaNI GTAC 9 cut(s) 293, 355, 846, 1078, 2149, 2790, 2838, 3127, 3889
RseI CAYNNNNRTG 1 cut(s) 1338
SacI GAGCTC 1 cut(s) 3692
SapI GCTCTTC 1 cut(s) 2802
Sau96I GGNCC 8 cut(s) 109, 216, 548, 750, 1089, 1136, 1187, 1831
ScaI AGTACT 3 cut(s) 356, 2791, 2839
ScrFI CCNGG 7 cut(s) 84, 391, 1854, 1998, 3356, 3554, 3638
SduI GDGCHC 3 cut(s) 641, 3692, 3794
SfcI CTRYAG 7 cut(s) 151, 808, 1572, 2068, 2535, 3943, 3957
SinI GGWCC 6 cut(s) 216, 548, 750, 1089, 1136, 1831
SmiMI CAYNNNNRTG 1 cut(s) 1338
SmlI CTYRAG 7 cut(s) 409, 2084, 2353, 2795, 2945, 3412, 3472
SmoI CTYRAG 7 cut(s) 409, 2084, 2353, 2795, 2945, 3412, 3472
SpeI ACTAGT 1 cut(s) 35
SsiI CCGC 5 cut(s) 613, 693, 1895, 2897, 3421
SspI AATATT 2 cut(s) 2913, 3844
SstI GAGCTC 1 cut(s) 3692
StyD4I CCNGG 7 cut(s) 82, 389, 1852, 1996, 3354, 3552, 3636
StyI CCWWGG 5 cut(s) 1053, 1741, 1827, 2475, 3693
TaiI ACGT 1 cut(s) 2849
TaqI TCGA 9 cut(s) 575, 635, 2041, 2511, 2735, 3033, 3642, 3788, 3984
TatI WGTACW 6 cut(s) 354, 845, 1077, 2789, 2837, 3126
TauI GCSGC 4 cut(s) 616, 695, 1898, 2899
TseFI GTSAC 7 cut(s) 166, 304, 673, 919, 2390, 3026, 3947
TseI GCWGC 6 cut(s) 153, 1637, 2067, 2546, 2641, 2727
Tsp45I GTSAC 7 cut(s) 166, 304, 673, 919, 2390, 3026, 3947
TspGWI ACGGA 5 cut(s) 288, 471, 474, 2409, 3889
Tth111I GACNNNGTC 3 cut(s) 310, 3497, 3638
Van91I CCANNNNNTGG 1 cut(s) 2608
Vha464I CTTAAG 2 cut(s) 2084, 3412
VpaK11BI GGWCC 6 cut(s) 216, 548, 750, 1089, 1136, 1831
VspI ATTAAT 1 cut(s) 2319
XagI CCTNNNNNAGG 1 cut(s) 2331
XceI RCATGY 2 cut(s) 13, 3955
XmaJI CCTAGG 1 cut(s) 1741
XmiI GTMKAC 2 cut(s) 147, 1106
XmnI GAANNNNTTC 1 cut(s) 428
ZrmI AGTACT 3 cut(s) 356, 2791, 2839
Zsp2I ATGCAT 2 cut(s) 895, 3883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.