Rroxscaffold_4G00277660

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003922
Physical Location & Seq
Forward (+)
991049 .. 998366
7318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00277660.1

Sequence Viewer

Length: 4329 bp
ATGAACACTGTTACCACACCTTCGTCCAATGCATTTAGTTATGATCAAGGCTTGGATGTGGCAGCAACTAGCTTTGTGGAAGCAAAACCGTATTATCCCTCTTATTTATCGCCCGCAATTCATGGTGATAGTCCTATGGTGGCTCCTGATCAACCTAGTTATGATTGGCTATCTACTTCTCAATTTGCTCCCTTGGATGGGTCGTCTCACAAAGAGTACACTCAACGGCCCTCTTCTTCAAAGTATACAGCTCAGTGGGGTGGCTCATGGAATGGACCAGCAGAGTGGGAGCAGGGTAAACAGGGACAATTTGATGGAAGTTTCGGCCCAAAGGAGACTGATGTATGTAGTTTTCCATATAAGAATTACCTGAACCAAGAACCTCATTCCTCCAATAGCTTGAAATCATACGGTGTAAATGAACTAGCATCCTGCAATATCCCAGATTGGGATGGATCTGTAAATGCAGAGCATTTAGGTGATAAATCCTTTGTGGGGAGAAAGTCCAAATTCAGCCCCATTGAATTTACAAAACCGACTATCGGATCCCTTTCAGGGGTTCCAGAAACCACTTCTAAGGCACCATCTTCACCTTTTGTTGCGAAGTCCACATTTGGTGTCTCATGTGAGAAAAGACAGCATGATGCTAGTTGGAATGATATCACACCTATTTCGAAGTCTTCCCCTGCCTTTATCATCAGACCACCGGCTATAGGCACTAAATCTTCAGAACCAGAGATAGGTCTATTCAAAAGATTGAATTCCGGAAGAGATGCAGCCAATGCAGATCATGGTGGTTATATTCCCTCACTGGAGTCTCATCTCCCACGAAGATTTGAAGACAAAGTTCCTTTTGATTCAAGCCAACTCGCCATTCACCTAGGAAGGAATGATCCCTTCTCTGTGGAATCTTCATCAACAAAAGACAAAGAGCTGTCGAATAATGGAAGTATCAACAATGATGCCTTGGACCATTTGTTTAAAGTAAAACCTGGACTTCCAAATTCTCATGTTAATCCTGATGGTTTTGATGCGGCACTTAATATCAATGATTCCATCAATTCTTTTCTGAATTCCTCTGAGAATGTGGATCCCAACAATCCAGCTGTGGACTCACCTTGTTGGAAGGGAGTTCCTGGTGAAAATATCTCCACCCAAAAGCCGGTTGAGTACCATGAATTTGGATGGCTGGGGAATGATTTGCTCGGAAATGGTTTGCCACTTCATTTGAAGAAATCTTCGGTGGCAAATTCTGCTTTTGGAGAACATAAATTAGATGATACCATGAAGACTGCCTGTTACCCTGAATCAAGCCATGATAGAGGGCTTCAAGATTACATTAATACCCCTAATTCAGGGAGTGGAGACAAGTCCTCAAGTCTCTTTGAGCATTCTCACACTGTGCAACAAGGTCACGGAGAAGGTGGATTAACAACTGAAGCAAATAACACAAAATGGAGTGTTGGTGCAGATGTTGAATTGAATGTCAATGACACCTTGGAATGTGGTTCATCTCATACCTCTCCTGCTGTCGAAAATATATTTTGTTCTCCTTCTGTAGAGGATGCTGCTACAAAGCTCACCAAATCAAATGGGGAAGAATCAAATATGAATATGGATGTCCAGATGCTGGTTAATACAATGAATAGCTTGTCAGAATTGCTGCTTGTTAATTGTTCAAACAGTTCACTTCAGTTGAAGAAAAAAGATCTTGAATCCCTAAAAGCTGTAATCAATAACTTGAATAGCTGCATATCGAAGCATGATGAAAACTTGTTGTCAATGCAAGAATCACCCTCTTTCCAGCAACGTACTCTCAAGTATATTGAAGAGCAAGAATCACCCCCTCTCCAGCAAAGTACTCTTACGTATATTGAAGAGTTATGCAAGCCAAATAAGGTTTTGAGTCCAGACAGGCCGCAGTTGACCAAAATATTTGCTCCTAGGATTCAGGATCCACTTCATCTTGAGGGTGTTCAGAAAGTAAAGAAGTATGATAGTTTTGTTAAGAATGATAATGAAGTTATAGGTTCTGTTTCTGCAAAAATTGACATTGACTTGCTGAAACAAGATAAGATGACTCAGGCTGTAAAGAAGATTCTGAGTGAGAATTTCCATACTGAGGATACACACCCTCAGACTCTCTTGTACAAGAATCTATGGCTTGAAGCTGAAGCTGTAATATGTTCCACCAATTACAAGGCTCGTTTTAATCGTCTGAAAAATGAAATGGAGAAGTGCAAGGCTGACCAATCCAAAGATGTGTTTGAACACACTGCTGATATGATGACGCAATCAAGGTCTGAAGTTTACCCTAATTCAAGCCCAGTTGAGAAGTTGACACCTGAAGTTCAGGGTTGTCCAAAGTTTAAACTCCAGGAGTCTCCTACTCTAAACCGAGAAGATGAAGATGTTATGGCAAGATTTCATATCTTAAGGAACCGCATTGAGGACTCACATTCCACGAATGCTACCAATGGGGATGAATCCAGCTCAAGTCTTTCTCCTGTTCCCGACAAGGTCGACGAGGTTGCACCTGATGCAGGTGCTAGGCTCTCACCTAGGATCTCCATCCAGGACTCTCCCACATTAAGCATAATTGGCCGGACAAATGATTATGAGGCTTCTGTTATGGCCAGATTTCATATTTTAAGAGACCGGGTCGAGAACTCAAAATTCAGTAGTGATGCCAATGTGGAGGATACAGTGAGCTCCAAGGCCTCTCATGAACACAAGGCTGAGGAAGTTGCACCTGTAACAAGTGATGATGGCTCAATTCAGGAGTTTAGTATTCAGGATTACCTTGTTTCGAGCACAACCGGCCATGCAAATCAGTATGATGATGGCTCAATTCAGGAGTTAAATATTCAGGATTACCTTGATTCAACCACAACGGGCCATGCATTTCAGTATGAGGATTCTGTTTTGGCCAGATTTAATATCCTTAAATCTCGGGTTGACAACTGTAGTGACTTGCCTACAGTAGAGCACTCTCTGGAAAATGTCGATCTTGGATATGCTGGTAAGAGAAACCTTAGACCTATTATTTGCAACAGATCAGAGGATGGAAGTTCAGATGTAAAAGAGCAACCTGTTTTGCAGAGTCACATAGCTGACAATAACGAAGGCAAGTTTGTGACTGCAAAGGAGTTCCGTCTGTTCATTGAGGATGATCCCAGCCACAGCATCAACAGGCCTGCGAATCAGCTCCCTGCCGGTTGTCCTGATCGGAACTCTTCATCAGATTGGGAACATGTCATGAAGGAGGAGGTCATCTATGTTATCCCCATTCCACTTGTCTTGTTCTTCTACTTGCTCAAATTACATGTTAGCTTTGACGAGCTAGCAACCATGACATTGCAGCTTAGCTGGCTGGTGGCTGCTTTCTCACTTGCCACTGCAGCTGGGTTGCTTAATTTCCTGCTCCAAGTTGGGTTTTATAGAAGTTCATGTAACGTAGCGGAGTTCATTGTGAAGGAGGAAGTTCGAAAAGGGGCGATTGGAAATCGAGGAGTGGCAGCTGGTCTTGTGAGACTGCACTTTCATGACTGCTTTGTTAGGGGTTGTGATGCATCAGTGCTGATTGATTCAACTTCCTCAAACACAGCAGAAAAAGATGCACCAGCAAACAACCCGAGTCTACGAGGGTTTGAAGTCATCGACAATGCCAAGGCCAGACTTGAATCTATATGCAAAGGTGTCGTATCATGTGCTGATATAGTTGCTTTTGCAGCAAGGGACAGCGTTGAACTTACTGGAGGACTTGGCTATGACGTTCCTGCTGGAAGAAGAGATGGCAGAGTTTCAGTAGCTTCAGACACATTCACAAACTTGCCTCCTCCAACTGCCAATGTCAACCAACTCACTCAACTTTTTTCAAACAAGGGTTTCACGCAAGATGAAATGGTCACTCTCTCTGGAGCACACACAATTGGGCGCTCTCACTGCAGTTCTTTCAGTAATAGATTGTACAATTTCAATGCAACAACAAAGCAGGACCCTAGTTTAGATCCCAGGTACGCTGCGCAGTTGAAGCAGCAATGTCCGCAAGGCAACACAAATCCAAACTTGGTGGTTGCCATGAACCCTTTCAGCCCTTTCACAACCGATGTATCCTACTACGCTGACATTTTGGCAAACCGAGGCTTGTTCACATCAGACCAAACTCTCATCACAAACTCTGCAACCGCAAACCAAGTTAATCAAAATGCCAGGAATCCCTTCCTGTGGAATAACAAATTTGCTGCTGCAATGGTGAAGATGGGACGACTCGGTGTCCTGACTGGCAATGCTGGTGAGATTCGGGAAAAGTGCAAGGTCTTTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1442

Amino Acids

158.48

Weight (kDa)

5.24

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 1158 - 1406 5.7e-86 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2534
Acc16I TGCGCA 1 cut(s) 4026
Acc36I ACCTGC 1 cut(s) 2534
AccB1I GGYRCC 1 cut(s) 580
AccB7I CCANNNNNTGG 1 cut(s) 1630
AccI GTMKAC 3 cut(s) 245, 2523, 3640
AccIII TCCGGA 1 cut(s) 764
AciI CCGC 7 cut(s) 114, 1034, 1919, 2443, 3461, 4046, 4188
AcoI YGGCCR 4 cut(s) 2602, 2634, 2821, 2928
AcuI CTGAAG 7 cut(s) 711, 1458, 1676, 2193, 2325, 2367, 3798
AdeI CACNNNGTG 1 cut(s) 1402
AfaI GTAC 7 cut(s) 218, 1172, 1813, 1861, 2150, 3971, 4019
AflII CTTAAG 1 cut(s) 2434
AflIII ACRYGT 2 cut(s) 3253, 3325
AhdI GACNNNNNGTC 1 cut(s) 4274
AjnI CCWGG 6 cut(s) 991, 1135, 2376, 2574, 4013, 4211
AjuI GAANNNNNNNTTGG 2 cut(s) 858, 890
Alw21I GWGCWC 4 cut(s) 2714, 2816, 2991, 3925
Alw26I GTCTC 9 cut(s) 210, 329, 625, 822, 1359, 1385, 2388, 2649, 3526
AlwNI CAGNNNCTG 1 cut(s) 1630
Ama87I CYCGRG 2 cut(s) 2952, 3634
Aor13HI TCCGGA 1 cut(s) 764
ArsI GACNNNNNNTTYG 2 cut(s) 3030, 3062
AseI ATTAAT 1 cut(s) 1341
Asp700I GAANNNNTTC 2 cut(s) 4088, 4220
AspA2I CCTAGG 3 cut(s) 880, 1943, 2561
AspLEI GCGC 2 cut(s) 3939, 4027
AspS9I GGNCC 6 cut(s) 228, 275, 326, 970, 2896, 3997
AsuC2I CCSGG 1 cut(s) 2660
AsuII TTCGAA 2 cut(s) 674, 3487
AsuNHI GCTAGC 1 cut(s) 3343
AvaI CYCGRG 2 cut(s) 2952, 3634
AvaII GGWCC 3 cut(s) 275, 970, 3997
AvrII CCTAGG 3 cut(s) 880, 1943, 2561
BaeI ACNNNNGTAYC 2 cut(s) 4095, 4128
BalI TGGCCA 2 cut(s) 2636, 2930
BamHI GGATCC 3 cut(s) 545, 1090, 1954
BanI GGYRCC 1 cut(s) 580
BanII GRGCYC 1 cut(s) 2714
BbsI GAAGAC 3 cut(s) 672, 846, 1295
Bbv12I GWGCWC 4 cut(s) 2714, 2816, 2991, 3925
BbvCI CCTCAGC 1 cut(s) 2739
BceAI ACGGC 1 cut(s) 242
BcgI CGANNNNNNTGC 4 cut(s) 2513, 2547, 3682, 3716
BciT130I CCWGG 6 cut(s) 993, 1137, 2378, 2576, 4015, 4213
BciVI GTATCC 3 cut(s) 2119, 2695, 4123
BclI TGATCA 2 cut(s) 43, 148
BcnI CCSGG 1 cut(s) 2660
BcoDI GTCTC 9 cut(s) 210, 329, 625, 822, 1359, 1385, 2388, 2649, 3526
BfmI CTRYAG 6 cut(s) 711, 1557, 2965, 2979, 3399, 3946
BfoI RGCGCY 1 cut(s) 3940
BfrI CTTAAG 1 cut(s) 2434
BfuAI ACCTGC 1 cut(s) 2534
BfuI GTATCC 3 cut(s) 2119, 2695, 4123
BglII AGATCT 1 cut(s) 1708
BlnI CCTAGG 3 cut(s) 880, 1943, 2561
BlpI GCTNAGC 1 cut(s) 3365
BmcAI AGTACT 1 cut(s) 1861
Bme1390I CCNGG 7 cut(s) 993, 1137, 2378, 2576, 2660, 4015, 4213
Bme18I GGWCC 3 cut(s) 275, 970, 3997
BmeRI GACNNNNNGTC 1 cut(s) 4274
BmeT110I CYCGRG 2 cut(s) 2952, 3634
BmgT120I GGNCC 6 cut(s) 228, 275, 326, 970, 2896, 3997
BmiI GGNNCC 8 cut(s) 144, 547, 561, 582, 1092, 1956, 2441, 3999
BmrFI CCNGG 7 cut(s) 993, 1137, 2378, 2576, 2660, 4015, 4213
BmrI ACTGGG 1 cut(s) 2321
BmtI GCTAGC 1 cut(s) 3347
BmuI ACTGGG 1 cut(s) 2321
BpiI GAAGAC 3 cut(s) 672, 846, 1295
BpmI CTGGAG 5 cut(s) 833, 1835, 2360, 3777, 3939
Bpu10I CCTNAGC 1 cut(s) 2739
Bpu1102I GCTNAGC 1 cut(s) 3365
Bpu14I TTCGAA 2 cut(s) 674, 3487
BpuEI CTTGAG 4 cut(s) 1360, 1802, 1988, 2479
BpuMI CCSGG 1 cut(s) 2660
BsaAI YACGTR 1 cut(s) 1869
BsaBI GATNNNNATC 1 cut(s) 2570
BsaI GGTCTC 1 cut(s) 2649
BsaWI WCCGGW 1 cut(s) 764
BsaXI ACNNNNNCTCC 4 cut(s) 1450, 1480, 1833, 1863
Bse118I RCCGGY 4 cut(s) 706, 1162, 2819, 3215
Bse1I ACTGG 4 cut(s) 816, 2327, 3760, 4288
Bse3DI GCAATG 4 cut(s) 3356, 4046, 4257, 4294
Bse8I GATNNNNATC 1 cut(s) 2570
BseAI TCCGGA 1 cut(s) 764
BseBI CCWGG 6 cut(s) 993, 1137, 2378, 2576, 4015, 4213
BseJI GATNNNNATC 1 cut(s) 2570
BseMI GCAATG 4 cut(s) 3356, 4046, 4257, 4294
BseMII CTCAG 7 cut(s) 266, 1071, 2093, 2096, 2112, 2150, 2730
BseNI ACTGG 4 cut(s) 816, 2327, 3760, 4288
BseRI GAGGAG 3 cut(s) 3281, 3525, 3828
BseYI CCCAGC 3 cut(s) 1189, 3176, 3404
BsgI GTGCAG 2 cut(s) 1488, 3521
BshNI GGYRCC 1 cut(s) 580
BsiHKAI GWGCWC 4 cut(s) 2714, 2816, 2991, 3925
BsiHKCI CYCGRG 2 cut(s) 2952, 3634
BsiSI CCGG 7 cut(s) 707, 765, 1163, 2605, 2659, 2820, 3216
BslFI GGGAC 3 cut(s) 318, 3752, 4278
BsmAI GTCTC 9 cut(s) 210, 329, 625, 822, 1359, 1385, 2388, 2649, 3526
BsmBI CGTCTC 1 cut(s) 210
BsmFI GGGAC 3 cut(s) 318, 3752, 4278
BsmI GAATGC 2 cut(s) 1390, 2473
Bso31I GGTCTC 1 cut(s) 2649
BsoBI CYCGRG 2 cut(s) 2952, 3634
Bsp119I TTCGAA 2 cut(s) 674, 3487
Bsp1286I GDGCHC 4 cut(s) 2714, 2816, 2991, 3925
Bsp13I TCCGGA 1 cut(s) 764
Bsp1407I TGTACA 2 cut(s) 2148, 3969
Bsp1720I GCTNAGC 1 cut(s) 3365
BspACI CCGC 7 cut(s) 114, 1034, 1919, 2443, 3461, 4046, 4188
BspCNI CTCAG 7 cut(s) 265, 1072, 2094, 2095, 2113, 2149, 2731
BspEI TCCGGA 1 cut(s) 764
BspHI TCATGA 3 cut(s) 2725, 3258, 3544
BspLI GGNNCC 8 cut(s) 144, 547, 561, 582, 1092, 1956, 2441, 3999
BspMAI CTGCAG 2 cut(s) 3403, 3950
BspMI ACCTGC 1 cut(s) 2534
BspOI GCTAGC 1 cut(s) 3347
BspQI GCTCTTC 1 cut(s) 1824
BspT104I TTCGAA 2 cut(s) 674, 3487
BspT107I GGYRCC 1 cut(s) 580
BspTI CTTAAG 1 cut(s) 2434
BspTNI GGTCTC 1 cut(s) 2649
BsrDI GCAATG 4 cut(s) 3356, 4046, 4257, 4294
BsrFI RCCGGY 4 cut(s) 706, 1162, 2819, 3215
BsrGI TGTACA 2 cut(s) 2148, 3969
BsrI ACTGG 4 cut(s) 816, 2327, 3760, 4288
BssAI RCCGGY 4 cut(s) 706, 1162, 2819, 3215
BssNAI GTATAC 1 cut(s) 246
BssT1I CCWWGG 8 cut(s) 192, 880, 966, 1497, 1943, 2561, 2715, 3669
Bst1107I GTATAC 1 cut(s) 246
Bst2UI CCWGG 6 cut(s) 993, 1137, 2378, 2576, 4015, 4213
Bst4CI ACNGT 8 cut(s) 10, 90, 413, 1402, 1685, 2707, 2966, 2983
Bst6I CTCTTC 6 cut(s) 238, 763, 1824, 1872, 3241, 3784
BstAFI CTTAAG 1 cut(s) 2434
BstAPI GCANNNNNTGC 3 cut(s) 782, 1253, 2540
BstAUI TGTACA 2 cut(s) 2148, 3969
BstBAI YACGTR 1 cut(s) 1869
BstBI TTCGAA 2 cut(s) 674, 3487
BstC8I GCNNGC 5 cut(s) 114, 1889, 3198, 3345, 3371
BstENI CCTNNNNNAGG 2 cut(s) 1353, 2541
BstH2I RGCGCY 1 cut(s) 3940
BstHHI GCGC 2 cut(s) 3939, 4027
BstMAI GTCTC 9 cut(s) 210, 329, 625, 822, 1359, 1385, 2388, 2649, 3526
BstNI CCWGG 6 cut(s) 993, 1137, 2378, 2576, 4015, 4213
BstNSI RCATGY 2 cut(s) 3257, 3329
BstSCI CCNGG 7 cut(s) 991, 1135, 2376, 2574, 2658, 4013, 4211
BstSFI CTRYAG 6 cut(s) 711, 1557, 2965, 2979, 3399, 3946
BstSNI TACGTA 1 cut(s) 1869
BstV2I GAAGAC 3 cut(s) 672, 846, 1295
BstX2I RGATCY 7 cut(s) 455, 545, 1090, 1708, 1954, 2565, 4009
BstXI CCANNNNNNTGG 2 cut(s) 285, 1181
BstYI RGATCY 7 cut(s) 455, 545, 1090, 1708, 1954, 2565, 4009
BstZ17I GTATAC 1 cut(s) 246
BsuI GTATCC 3 cut(s) 2119, 2695, 4123
BtsI GCAGTG 3 cut(s) 2274, 3396, 3943
BtsIMutI CAGTG 9 cut(s) 6, 260, 809, 1398, 2274, 2712, 3396, 3582, 3943
BveI ACCTGC 1 cut(s) 2534
Cac8I GCNNGC 5 cut(s) 114, 1889, 3198, 3345, 3371
CaiI CAGNNNCTG 1 cut(s) 1630
CciI TCATGA 3 cut(s) 2725, 3258, 3544
CfoI GCGC 2 cut(s) 3939, 4027
Cfr10I RCCGGY 4 cut(s) 706, 1162, 2819, 3215
Cfr13I GGNCC 6 cut(s) 228, 275, 326, 970, 2896, 3997
CseI GACGC 1 cut(s) 2299
Csp6I GTAC 7 cut(s) 217, 1171, 1812, 1860, 2149, 3970, 4018
CspCI CAANNNNNGTGG 6 cut(s) 1209, 1244, 3282, 3317, 4053, 4088
CviQI GTAC 7 cut(s) 217, 1171, 1812, 1860, 2149, 3970, 4018
DraI TTTAAA 2 cut(s) 982, 2371
DraIII CACNNNGTG 1 cut(s) 1402
DriI GACNNNNNGTC 1 cut(s) 4274
EaeI YGGCCR 4 cut(s) 2602, 2634, 2821, 2928
Eam1104I CTCTTC 6 cut(s) 238, 763, 1824, 1872, 3241, 3784
Eam1105I GACNNNNNGTC 1 cut(s) 4274
EarI CTCTTC 6 cut(s) 238, 763, 1824, 1872, 3241, 3784
Ecl136II GAGCTC 1 cut(s) 2712
Eco105I TACGTA 1 cut(s) 1869
Eco130I CCWWGG 8 cut(s) 192, 880, 966, 1497, 1943, 2561, 2715, 3669
Eco147I AGGCCT 2 cut(s) 2720, 3196
Eco24I GRGCYC 1 cut(s) 2714
Eco31I GGTCTC 1 cut(s) 2649
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 3 cut(s) 275, 970, 3997
Eco53kI GAGCTC 1 cut(s) 2712
Eco57I CTGAAG 7 cut(s) 711, 1458, 1676, 2193, 2325, 2367, 3798
Eco88I CYCGRG 2 cut(s) 2952, 3634
EcoICRI GAGCTC 1 cut(s) 2712
EcoNI CCTNNNNNAGG 2 cut(s) 1353, 2541
EcoO109I RGGNCCY 1 cut(s) 3997
EcoRI GAATTC 2 cut(s) 760, 1072
EcoRII CCWGG 6 cut(s) 991, 1135, 2376, 2574, 4013, 4211
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 8 cut(s) 192, 880, 966, 1497, 1943, 2561, 2715, 3669
EcoT22I ATGCAT 3 cut(s) 34, 2905, 3574
EcoT38I GRGCYC 1 cut(s) 2714
ErhI CCWWGG 8 cut(s) 192, 880, 966, 1497, 1943, 2561, 2715, 3669
Esp3I CGTCTC 1 cut(s) 210
FaqI GGGAC 3 cut(s) 318, 3752, 4278
FauI CCCGC 1 cut(s) 121
FbaI TGATCA 2 cut(s) 43, 148
FblI GTMKAC 3 cut(s) 245, 2523, 3640
FriOI GRGCYC 1 cut(s) 2714
FspI TGCGCA 1 cut(s) 4026
GlaI GCGC 2 cut(s) 3938, 4026
GsaI CCCAGC 3 cut(s) 1193, 3180, 3408
GsuI CTGGAG 5 cut(s) 833, 1835, 2360, 3777, 3939
HaeII RGCGCY 1 cut(s) 3940
HapII CCGG 7 cut(s) 707, 765, 1163, 2605, 2659, 2820, 3216
HgaI GACGC 1 cut(s) 2299
HhaI GCGC 2 cut(s) 3939, 4027
Hin6I GCGC 2 cut(s) 3937, 4025
HinP1I GCGC 2 cut(s) 3937, 4025
HincII GTYRAC 5 cut(s) 1926, 2340, 2524, 2959, 3856
HindII GTYRAC 5 cut(s) 1926, 2340, 2524, 2959, 3856
HpaII CCGG 7 cut(s) 707, 765, 1163, 2605, 2659, 2820, 3216
Hpy99I CGWCG 1 cut(s) 2528
HpyCH4III ACNGT 8 cut(s) 10, 90, 413, 1402, 1685, 2707, 2966, 2983
HpyCH4IV ACGT 4 cut(s) 1810, 1868, 3456, 3774
HpySE526I ACGT 4 cut(s) 1810, 1868, 3456, 3774
HspAI GCGC 2 cut(s) 3937, 4025
Kpn2I TCCGGA 1 cut(s) 764
Ksp22I TGATCA 2 cut(s) 43, 148
LguI GCTCTTC 1 cut(s) 1824
LmnI GCTCC 8 cut(s) 148, 193, 289, 1945, 2717, 3213, 3429, 3920
MaeII ACGT 4 cut(s) 1810, 1868, 3456, 3774
MaeIII GTNAC 9 cut(s) 10, 1298, 1412, 2755, 2969, 3104, 3136, 3452, 3907
MfeI CAATTG 1 cut(s) 3930
MflI RGATCY 7 cut(s) 455, 545, 1090, 1708, 1954, 2565, 4009
MhlI GDGCHC 4 cut(s) 2714, 2816, 2991, 3925
MlsI TGGCCA 2 cut(s) 2636, 2930
MluNI TGGCCA 2 cut(s) 2636, 2930
MmeI TCCRAC 3 cut(s) 632, 1103, 3866
Mox20I TGGCCA 2 cut(s) 2636, 2930
Mph1103I ATGCAT 3 cut(s) 34, 2905, 3574
MroI TCCGGA 1 cut(s) 764
MroXI GAANNNNTTC 2 cut(s) 4088, 4220
MscI TGGCCA 2 cut(s) 2636, 2930
MslI CAYNNNNRTG 2 cut(s) 477, 3543
Msp20I TGGCCA 2 cut(s) 2636, 2930
MspA1I CMGCKG 3 cut(s) 1106, 3404, 3521
MspCI CTTAAG 1 cut(s) 2434
MspI CCGG 7 cut(s) 707, 765, 1163, 2605, 2659, 2820, 3216
MspR9I CCNGG 7 cut(s) 993, 1137, 2378, 2576, 2660, 4015, 4213
MssI GTTTAAAC 1 cut(s) 2371
MunI CAATTG 1 cut(s) 3930
Mva1269I GAATGC 2 cut(s) 1390, 2473
MvaI CCWGG 6 cut(s) 993, 1137, 2378, 2576, 4015, 4213
NciI CCSGG 1 cut(s) 2660
NheI GCTAGC 1 cut(s) 3343
NlaIV GGNNCC 8 cut(s) 144, 547, 561, 582, 1092, 1956, 2441, 3999
NmuCI GTSAC 5 cut(s) 1412, 2969, 3104, 3136, 3907
NsbI TGCGCA 1 cut(s) 4026
NsiI ATGCAT 3 cut(s) 34, 2905, 3574
NspI RCATGY 2 cut(s) 3257, 3329
NspV TTCGAA 2 cut(s) 674, 3487
PagI TCATGA 3 cut(s) 2725, 3258, 3544
PaqCI CACCTGC 1 cut(s) 2534
PceI AGGCCT 2 cut(s) 2720, 3196
PciI ACATGT 2 cut(s) 3253, 3325
PciSI GCTCTTC 1 cut(s) 1824
PcsI WCGNNNNNNNCGW 1 cut(s) 2212
PctI GAATGC 2 cut(s) 1390, 2473
PdmI GAANNNNTTC 2 cut(s) 4088, 4220
PflFI GACNNNGTC 2 cut(s) 2519, 2660
PflMI CCANNNNNTGG 1 cut(s) 1630
PfoI TCCNGGA 2 cut(s) 2376, 2574
PmeI GTTTAAAC 1 cut(s) 2371
Ppu21I YACGTR 1 cut(s) 1869
PpuMI RGGWCCY 1 cut(s) 3997
PscI ACATGT 2 cut(s) 3253, 3325
PshBI ATTAAT 1 cut(s) 1341
Psp124BI GAGCTC 1 cut(s) 2714
Psp5II RGGWCCY 1 cut(s) 3997
Psp6I CCWGG 6 cut(s) 991, 1135, 2376, 2574, 4013, 4211
PspFI CCCAGC 3 cut(s) 1189, 3176, 3404
PspGI CCWGG 6 cut(s) 991, 1135, 2376, 2574, 4013, 4211
PspN4I GGNNCC 8 cut(s) 144, 547, 561, 582, 1092, 1956, 2441, 3999
PspPI GGNCC 6 cut(s) 228, 275, 326, 970, 2896, 3997
PspPPI RGGWCCY 1 cut(s) 3997
PstI CTGCAG 2 cut(s) 3403, 3950
PstNI CAGNNNCTG 1 cut(s) 1630
PsuI RGATCY 7 cut(s) 455, 545, 1090, 1708, 1954, 2565, 4009
PsyI GACNNNGTC 2 cut(s) 2519, 2660
PvuII CAGCTG 3 cut(s) 1106, 3404, 3521
RsaI GTAC 7 cut(s) 218, 1172, 1813, 1861, 2150, 3971, 4019
RsaNI GTAC 7 cut(s) 217, 1171, 1812, 1860, 2149, 3970, 4018
RseI CAYNNNNRTG 2 cut(s) 477, 3543
SacI GAGCTC 1 cut(s) 2714
SalI GTCGAC 1 cut(s) 2522
SapI GCTCTTC 1 cut(s) 1824
Sau96I GGNCC 6 cut(s) 228, 275, 326, 970, 2896, 3997
ScaI AGTACT 1 cut(s) 1861
ScrFI CCNGG 7 cut(s) 993, 1137, 2378, 2576, 2660, 4015, 4213
SduI GDGCHC 4 cut(s) 2714, 2816, 2991, 3925
SfcI CTRYAG 6 cut(s) 711, 1557, 2965, 2979, 3399, 3946
SfuI TTCGAA 2 cut(s) 674, 3487
SinI GGWCC 3 cut(s) 275, 970, 3997
SmiMI CAYNNNNRTG 2 cut(s) 477, 3543
SmlI CTYRAG 5 cut(s) 1375, 1817, 1967, 2434, 2494
SmoI CTYRAG 5 cut(s) 1375, 1817, 1967, 2434, 2494
SnaBI TACGTA 1 cut(s) 1869
SseBI AGGCCT 2 cut(s) 2720, 3196
SsiI CCGC 7 cut(s) 114, 1034, 1919, 2443, 3461, 4046, 4188
SspI AATATT 2 cut(s) 1935, 2866
SstI GAGCTC 1 cut(s) 2714
StuI AGGCCT 2 cut(s) 2720, 3196
StyD4I CCNGG 7 cut(s) 991, 1135, 2376, 2574, 2658, 4013, 4211
StyI CCWWGG 8 cut(s) 192, 880, 966, 1497, 1943, 2561, 2715, 3669
TaaI ACNGT 8 cut(s) 10, 90, 413, 1402, 1685, 2707, 2966, 2983
TaiI ACGT 4 cut(s) 1813, 1871, 3459, 3777
TatI WGTACW 4 cut(s) 216, 1859, 2148, 3969
TauI GCSGC 2 cut(s) 1037, 1921
TscAI CASTG 9 cut(s) 13, 260, 816, 1405, 2281, 2712, 3403, 3582, 3950
TseFI GTSAC 5 cut(s) 1412, 2969, 3104, 3136, 3907
Tsp45I GTSAC 5 cut(s) 1412, 2969, 3104, 3136, 3907
TspGWI ACGGA 2 cut(s) 1431, 3143
TspRI CASTG 9 cut(s) 13, 260, 816, 1405, 2281, 2712, 3403, 3582, 3950
Tth111I GACNNNGTC 2 cut(s) 2519, 2660
Van91I CCANNNNNTGG 1 cut(s) 1630
Vha464I CTTAAG 1 cut(s) 2434
VpaK11BI GGWCC 3 cut(s) 275, 970, 3997
VspI ATTAAT 1 cut(s) 1341
XagI CCTNNNNNAGG 2 cut(s) 1353, 2541
XceI RCATGY 2 cut(s) 3257, 3329
XmaJI CCTAGG 3 cut(s) 880, 1943, 2561
XmiI GTMKAC 3 cut(s) 245, 2523, 3640
XmnI GAANNNNTTC 2 cut(s) 4088, 4220
ZrmI AGTACT 1 cut(s) 1861
Zsp2I ATGCAT 3 cut(s) 34, 2905, 3574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.