RLG00000031735

phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009090
Physical Location & Seq
Reverse (-)
7882101 .. 7883447
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031735

Sequence Viewer

Length: 1242 bp
ATGTTTGGTCACAAGAAAAAGATGGATAACATTATTGGCAAGCACAGCATTGCGAAAAGATGGAAGAAACTAAGCGGCGAAGACAACTGGGAAGGCATGTTGGATCCTCTCGACATTGATCTTCGAAAGTACATAATTCACTATGGCGAAATGGCTCAATCTACATATGATGCTTTCAACACAGAGAGGGCATCAAAGTTTGCAGGAAGTAGCAGGTACGCCAGGAAGACCTTCTTCTCCAAAGTTGGTTTAGAGAAGGGCAATGAATTTAGGTACAACGTGACCAAGTTTGTGTATGCAACTTCACAAATTCAAGTCCCAGAAGCGTTTATCGTTAAGTCTTTGTCAAGGGAGGCTTGGAGCAAAGAATCAAATTGGATGGGGTATGTTGCTGTGGCCACAGATGAGGGGAAGGCTGTGTTGGGCAGGAGGGACATTGTGATTGCTTGGAGAGGTACTATTCAGTCCTTGGAGTGGGTTAATGATTTGCAGTTCAATATGGTTTCTGCTTCAAAAATACTAGGTGAGGAGGCTGATGCTAAGGTGCACCAAGGTTGGTACTCCATCTATACTTCCGAAGATTCGCATTCGCCTTTCAACAAAACCAGTGCCAGAATTCAGGTTCTTGCTGAGGTGAGCAGACTAGTAGAGCAATACAAGGATGAGGAAATCAGCATAACCATGACAGGGCACAGTTTAGGAGCAGCAATTGCCACGCTAAGTGCAATTGACATAGTTTCCAACGGTGTCAACAAACCGAAAGACCAGACAACAAACAAACTCTCATCATGTCCAGTAACTGCCTTTGTCTTTGCTTGCCCTCGAGTCGGAGACTCAAACTTCAAGAAAGTCTTCTCTAGCTACAAAAACCTCCGAGTCCTGCGAGTTCGCAATGCACTCGATATTGTCCCCAACTACCCGTTTCTAATAGGCTATTCCGAGGTGGGAGAAGAGCTGGGAATCGACACTCGAAAGTCGAGCTACTTGAAGAGCCCTGGAAGCGTGGTGAGTTGGCATAACATGGAGGGGCATTTGCATGGAGTTGCAGGAACTCAAGGGAGTAAAGGAGGGTTCAAGTTGGAAGTGAAGCGTGACATAGCATTGGTTAACAAAAGCAATGATGCTCTCAAGGACGAGTATCTTGTGCCGGTTTCTTGGCGTTGTGAGAAGGACAAGGGGATGGTTCAAATGGATGATGGTTCTTGGCAGCTCATGGATCATGAGGGGGATTTTGATTCTTGA

Protein Analysis

414

Amino Acids

46.41

Weight (kDa)

8.18

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 147 - 308 1.4e-37 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 204
AciI CCGC 1 cut(s) 75
AclWI GGATC 3 cut(s) 98, 111, 1224
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 3 cut(s) 266, 309, 615
AfaI GTAC 5 cut(s) 131, 218, 275, 457, 560
AfiI CCNNNNNNNGG 3 cut(s) 474, 687, 827
AgsI TTSAA 9 cut(s) 178, 314, 496, 513, 598, 844, 988, 1075, 1187
AhlI ACTAGT 1 cut(s) 643
AjnI CCWGG 2 cut(s) 221, 994
AjuI GAANNNNNNNTTGG 2 cut(s) 404, 436
AluBI AGCT 4 cut(s) 861, 955, 981, 1210
AluI AGCT 4 cut(s) 861, 955, 981, 1210
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 1 cut(s) 825
Alw44I GTGCAC 1 cut(s) 545
AlwI GGATC 3 cut(s) 98, 111, 1224
AlwNI CAGNNNCTG 1 cut(s) 800
Ama87I CYCGRG 1 cut(s) 822
AoxI GGCC 1 cut(s) 396
ApaLI GTGCAC 1 cut(s) 545
ApeKI GCWGC 2 cut(s) 704, 1207
ApoI RAATTY 3 cut(s) 266, 309, 615
Asp700I GAANNNNTTC 2 cut(s) 230, 851
AsuHPI GGTGA 3 cut(s) 536, 646, 1018
AsuII TTCGAA 1 cut(s) 124
AvaI CYCGRG 1 cut(s) 822
BaeGI GKGCMC 2 cut(s) 549, 693
BalI TGGCCA 1 cut(s) 398
BamHI GGATCC 1 cut(s) 103
BanII GRGCYC 1 cut(s) 995
BbsI GAAGAC 3 cut(s) 87, 233, 844
Bbv12I GWGCWC 1 cut(s) 549
BbvCI CCTCAGC 1 cut(s) 630
BbvI GCAGC 2 cut(s) 716, 1219
BccI CCATC 6 cut(s) 16, 54, 373, 572, 1174, 1190
BcgI CGANNNNNNTGC 2 cut(s) 880, 914
BciT130I CCWGG 2 cut(s) 223, 996
BcoDI GTCTC 1 cut(s) 825
BcuI ACTAGT 1 cut(s) 643
BfaI CTAG 3 cut(s) 521, 644, 858
BfuAI ACCTGC 1 cut(s) 204
BisI GCNGC 3 cut(s) 76, 705, 1208
BlsI GCNGC 3 cut(s) 77, 706, 1209
Bme1390I CCNGG 2 cut(s) 223, 996
BmeT110I CYCGRG 1 cut(s) 822
BmiI GGNNCC 1 cut(s) 105
BmrFI CCNGG 2 cut(s) 223, 996
BmrI ACTGGG 1 cut(s) 97
BmsI GCATC 4 cut(s) 160, 200, 526, 1111
BmuI ACTGGG 1 cut(s) 97
BpiI GAAGAC 3 cut(s) 87, 233, 844
Bpu10I CCTNAGC 2 cut(s) 540, 630
Bpu14I TTCGAA 1 cut(s) 124
BpuEI CTTGAG 2 cut(s) 1038, 1112
BsaJI CCNNGG 4 cut(s) 468, 550, 939, 994
Bsc4I CCNNNNNNNGG 3 cut(s) 474, 687, 827
Bse118I RCCGGY 1 cut(s) 1147
Bse1I ACTGG 3 cut(s) 92, 606, 794
Bse3DI GCAATG 4 cut(s) 48, 268, 898, 1123
BseBI CCWGG 2 cut(s) 223, 996
BseDI CCNNGG 4 cut(s) 468, 550, 939, 994
BseGI GGATG 4 cut(s) 384, 667, 1185, 1198
BseLI CCNNNNNNNGG 3 cut(s) 474, 687, 827
BseMI GCAATG 4 cut(s) 48, 268, 898, 1123
BseMII CTCAG 1 cut(s) 621
BseNI ACTGG 3 cut(s) 92, 606, 794
BseRI GAGGAG 1 cut(s) 542
BseSI GKGCMC 2 cut(s) 549, 693
BseXI GCAGC 2 cut(s) 716, 1219
BseYI CCCAGC 1 cut(s) 955
BshFI GGCC 1 cut(s) 398
BsiHKAI GWGCWC 1 cut(s) 549
BsiHKCI CYCGRG 1 cut(s) 822
BsiSI CCGG 1 cut(s) 1148
BslFI GGGAC 3 cut(s) 302, 446, 893
BslI CCNNNNNNNGG 3 cut(s) 474, 687, 827
BsmAI GTCTC 1 cut(s) 825
BsmFI GGGAC 3 cut(s) 302, 446, 893
BsmI GAATGC 1 cut(s) 586
BsnI GGCC 1 cut(s) 398
BsoBI CYCGRG 1 cut(s) 822
Bsp119I TTCGAA 1 cut(s) 124
Bsp1286I GDGCHC 3 cut(s) 549, 693, 995
Bsp143I GATC 3 cut(s) 103, 118, 1216
BspACI CCGC 1 cut(s) 75
BspANI GGCC 1 cut(s) 398
BspCNI CTCAG 1 cut(s) 622
BspHI TCATGA 1 cut(s) 1219
BspLI GGNNCC 1 cut(s) 105
BspMI ACCTGC 1 cut(s) 204
BspPI GGATC 3 cut(s) 98, 111, 1224
BspQI GCTCTTC 2 cut(s) 945, 983
BspT104I TTCGAA 1 cut(s) 124
BsrDI GCAATG 4 cut(s) 48, 268, 898, 1123
BsrFI RCCGGY 1 cut(s) 1147
BsrI ACTGG 3 cut(s) 92, 606, 794
BssAI RCCGGY 1 cut(s) 1147
BssECI CCNNGG 4 cut(s) 468, 550, 939, 994
BssMI GATC 3 cut(s) 103, 118, 1216
BssT1I CCWWGG 2 cut(s) 468, 550
Bst2UI CCWGG 2 cut(s) 223, 996
Bst4CI ACNGT 2 cut(s) 695, 746
Bst6I CTCTTC 2 cut(s) 945, 983
BstAPI GCANNNNNTGC 1 cut(s) 710
BstBI TTCGAA 1 cut(s) 124
BstC8I GCNNGC 2 cut(s) 41, 817
BstDEI CTNAG 4 cut(s) 71, 540, 630, 719
BstF5I GGATG 4 cut(s) 384, 667, 1185, 1198
BstKTI GATC 3 cut(s) 106, 121, 1219
BstMAI GTCTC 1 cut(s) 825
BstMBI GATC 3 cut(s) 103, 118, 1216
BstMWI GCNNNNNNNGC 3 cut(s) 45, 710, 999
BstNI CCWGG 2 cut(s) 223, 996
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 2 cut(s) 221, 994
BstSLI GKGCMC 2 cut(s) 549, 693
BstV1I GCAGC 2 cut(s) 716, 1219
BstV2I GAAGAC 3 cut(s) 87, 233, 844
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
BsuRI GGCC 1 cut(s) 398
BtsCI GGATG 4 cut(s) 384, 667, 1185, 1198
BtsIMutI CAGTG 1 cut(s) 613
BveI ACCTGC 1 cut(s) 204
Cac8I GCNNGC 2 cut(s) 41, 817
CaiI CAGNNNCTG 1 cut(s) 800
CciI TCATGA 1 cut(s) 1219
Cfr10I RCCGGY 1 cut(s) 1147
Csp6I GTAC 5 cut(s) 130, 217, 274, 456, 559
CviAII CATG 7 cut(s) 97, 682, 789, 1021, 1037, 1213, 1220
CviQI GTAC 5 cut(s) 130, 217, 274, 456, 559
DdeI CTNAG 4 cut(s) 71, 540, 630, 719
DpnI GATC 3 cut(s) 105, 120, 1218
DpnII GATC 3 cut(s) 103, 118, 1216
EaeI YGGCCR 1 cut(s) 396
Eam1104I CTCTTC 2 cut(s) 945, 983
EarI CTCTTC 2 cut(s) 945, 983
Eco130I CCWWGG 2 cut(s) 468, 550
Eco24I GRGCYC 1 cut(s) 995
Eco88I CYCGRG 1 cut(s) 822
EcoRI GAATTC 1 cut(s) 615
EcoRII CCWGG 2 cut(s) 221, 994
EcoT14I CCWWGG 2 cut(s) 468, 550
EcoT38I GRGCYC 1 cut(s) 995
ErhI CCWWGG 2 cut(s) 468, 550
FaeI CATG 7 cut(s) 100, 685, 792, 1024, 1040, 1216, 1223
FalI AAGNNNNNCTT 6 cut(s) 218, 250, 340, 372, 836, 868
FaqI GGGAC 3 cut(s) 302, 446, 893
FatI CATG 7 cut(s) 96, 681, 788, 1020, 1036, 1212, 1219
FauNDI CATATG 1 cut(s) 166
Fnu4HI GCNGC 3 cut(s) 76, 705, 1208
FokI GGATG 4 cut(s) 391, 674, 1192, 1205
FriOI GRGCYC 1 cut(s) 995
Fsp4HI GCNGC 3 cut(s) 76, 705, 1208
FspBI CTAG 3 cut(s) 521, 644, 858
GluI GCNGC 3 cut(s) 76, 705, 1208
GsaI CCCAGC 1 cut(s) 959
HaeIII GGCC 1 cut(s) 398
HapII CCGG 1 cut(s) 1148
Hin1II CATG 7 cut(s) 100, 685, 792, 1024, 1040, 1216, 1223
HincII GTYRAC 2 cut(s) 751, 1108
HindII GTYRAC 2 cut(s) 751, 1108
HinfI GANTC 7 cut(s) 368, 581, 825, 833, 876, 960, 1235
HpaI GTTAAC 1 cut(s) 1108
HpaII CCGG 1 cut(s) 1148
HphI GGTGA 3 cut(s) 536, 646, 1018
Hpy166II GTNNAC 3 cut(s) 547, 751, 1108
Hpy188I TCNGA 4 cut(s) 577, 830, 875, 940
Hpy188III TCNNGA 4 cut(s) 110, 844, 1220, 1239
Hpy8I GTNNAC 3 cut(s) 547, 751, 1108
HpyAV CCTTC 5 cut(s) 86, 241, 250, 406, 1162
HpyCH4III ACNGT 2 cut(s) 695, 746
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 8 cut(s) 203, 299, 490, 547, 725, 896, 1036, 1046
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 710, 999
HpyF3I CTNAG 4 cut(s) 71, 540, 630, 719
HpySE526I ACGT 1 cut(s) 279
Hsp92II CATG 7 cut(s) 100, 685, 792, 1024, 1040, 1216, 1223
KspAI GTTAAC 1 cut(s) 1108
Kzo9I GATC 3 cut(s) 103, 118, 1216
LguI GCTCTTC 2 cut(s) 945, 983
LmnI GCTCC 2 cut(s) 360, 701
Lsp1109I GCAGC 2 cut(s) 716, 1219
LweI GCATC 4 cut(s) 160, 200, 526, 1111
MaeI CTAG 3 cut(s) 521, 644, 858
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 4 cut(s) 8, 280, 796, 1091
MalI GATC 3 cut(s) 105, 120, 1218
MboI GATC 3 cut(s) 103, 118, 1216
MboII GAAGA 9 cut(s) 76, 92, 113, 226, 238, 590, 844, 962, 1000
MfeI CAATTG 2 cut(s) 708, 726
MflI RGATCY 1 cut(s) 103
MhlI GDGCHC 3 cut(s) 549, 693, 995
MlsI TGGCCA 1 cut(s) 398
MluCI AATT 7 cut(s) 135, 266, 309, 373, 615, 708, 726
MluNI TGGCCA 1 cut(s) 398
MlyI GAGTC 3 cut(s) 827, 834, 885
MmeI TCCRAC 4 cut(s) 81, 765, 808, 1059
Mox20I TGGCCA 1 cut(s) 398
MroXI GAANNNNTTC 2 cut(s) 230, 851
MscI TGGCCA 1 cut(s) 398
MseI TTAA 3 cut(s) 336, 480, 1107
MslI CAYNNNNRTG 2 cut(s) 680, 1035
Msp20I TGGCCA 1 cut(s) 398
MspI CCGG 1 cut(s) 1148
MspR9I CCNGG 2 cut(s) 223, 996
MunI CAATTG 2 cut(s) 708, 726
Mva1269I GAATGC 1 cut(s) 586
MvaI CCWGG 2 cut(s) 223, 996
MwoI GCNNNNNNNGC 3 cut(s) 45, 710, 999
NdeI CATATG 1 cut(s) 166
NdeII GATC 3 cut(s) 103, 118, 1216
NlaIII CATG 7 cut(s) 100, 685, 792, 1024, 1040, 1216, 1223
NlaIV GGNNCC 1 cut(s) 105
NmuCI GTSAC 3 cut(s) 8, 280, 1091
NspI RCATGY 1 cut(s) 100
NspV TTCGAA 1 cut(s) 124
PaeR7I CTCGAG 1 cut(s) 822
PagI TCATGA 1 cut(s) 1219
PciSI GCTCTTC 2 cut(s) 945, 983
PctI GAATGC 1 cut(s) 586
PdmI GAANNNNTTC 2 cut(s) 230, 851
PfeI GAWTC 4 cut(s) 368, 581, 960, 1235
PkrI GCNGC 3 cut(s) 77, 706, 1209
PleI GAGTC 3 cut(s) 827, 833, 884
PpsI GAGTC 3 cut(s) 827, 833, 884
Psp6I CCWGG 2 cut(s) 221, 994
PspFI CCCAGC 1 cut(s) 955
PspGI CCWGG 2 cut(s) 221, 994
PspN4I GGNNCC 1 cut(s) 105
PspXI VCTCGAGB 1 cut(s) 822
PstNI CAGNNNCTG 1 cut(s) 800
PsuI RGATCY 1 cut(s) 103
RsaI GTAC 5 cut(s) 131, 218, 275, 457, 560
RsaNI GTAC 5 cut(s) 130, 217, 274, 456, 559
RseI CAYNNNNRTG 2 cut(s) 680, 1035
SapI GCTCTTC 2 cut(s) 945, 983
SaqAI TTAA 3 cut(s) 336, 480, 1107
SatI GCNGC 3 cut(s) 76, 705, 1208
Sau3AI GATC 3 cut(s) 103, 118, 1216
SchI GAGTC 3 cut(s) 827, 834, 885
ScrFI CCNGG 2 cut(s) 223, 996
SduI GDGCHC 3 cut(s) 549, 693, 995
SfaNI GCATC 4 cut(s) 160, 200, 526, 1111
Sfr274I CTCGAG 1 cut(s) 822
SfuI TTCGAA 1 cut(s) 124
SlaI CTCGAG 1 cut(s) 822
SmiMI CAYNNNNRTG 2 cut(s) 680, 1035
SmlI CTYRAG 3 cut(s) 822, 1053, 1127
SmoI CTYRAG 3 cut(s) 822, 1053, 1127
SpeI ACTAGT 1 cut(s) 643
Sse9I AATT 7 cut(s) 135, 266, 309, 373, 615, 708, 726
SsiI CCGC 1 cut(s) 75
SspMI CTAG 3 cut(s) 521, 644, 858
StyD4I CCNGG 2 cut(s) 221, 994
StyI CCWWGG 2 cut(s) 468, 550
TaaI ACNGT 2 cut(s) 695, 746
TaiI ACGT 1 cut(s) 282
TaqI TCGA 7 cut(s) 111, 124, 823, 900, 963, 970, 977
TasI AATT 7 cut(s) 135, 266, 309, 373, 615, 708, 726
TatI WGTACW 1 cut(s) 129
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 4 cut(s) 368, 581, 960, 1235
Tru1I TTAA 3 cut(s) 336, 480, 1107
Tru9I TTAA 3 cut(s) 336, 480, 1107
TscAI CASTG 1 cut(s) 613
TseFI GTSAC 3 cut(s) 8, 280, 1091
TseI GCWGC 2 cut(s) 704, 1207
Tsp45I GTSAC 3 cut(s) 8, 280, 1091
TspDTI ATGAA 1 cut(s) 279
TspRI CASTG 1 cut(s) 613
VneI GTGCAC 1 cut(s) 545
XapI RAATTY 3 cut(s) 266, 309, 615
XceI RCATGY 1 cut(s) 100
XhoI CTCGAG 1 cut(s) 822
XmnI GAANNNNTTC 2 cut(s) 230, 851
XspI CTAG 3 cut(s) 521, 644, 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.