RLG00000032166

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010538
Physical Location & Seq
Reverse (-)
11745823 .. 11749116
3294 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032166

Sequence Viewer

Length: 780 bp
ATGCTTCAGCCCACTAGTAAAGCTTTTGGGGAGACTCCATTGTTATTGGGTTTCATGCTTCAGCCCACCAGTCCACATAGAAAGTGTGATGCCACGTTTATCATATTTTGGCGGGAACAAATCCCCGTCCAGCCCCCAACCGCCGATTTCCAACATTCAATACGGCGTGTCGTTTTTCAGATGGATCTTGATGCGAAAAGGTTTGGAAGAGGGCCTCGGGAACTTGGTGGTGCTGCCAATCTCATAAATCAGTACAAGCTTTGGCCTTATCATGAATTCTTTTGCAAACGGTCGCTTCCTTTATCAGAGACTCATTATCTTCATAATGTGGTGGGAGACACAGAAATCAGGAAAGGGGAGGGGATGAAATTGAATCAGCTATTTGAGAACACCCCTTGTATGAGAGAAAGGAACGCAAACATACATCCTTTCGATTTGGGTATGCTTAGTGAAGCTTTCCGAATAAAGGAGACAACTCCTGTTCATTTACCTTCTGCCGAGAAAGGGATCCGTACCTCAGTGGTGAAGTCAAAGGGTGAGTTTGAAGACAAGGAAAGGAAGCATAAAAAGAACAAAGACAAGGATAAGGATCGTAAGAAGCACAAAAAAGGTCGCCAGGACAACCACTGTGATGCTGATTACAGCAATAAGAATGTTACTAATGGCCTGAAAACTGTGCAGTTGAAGGAACAGCCAGATATGTTCATTCAGAAGAGGAGGCTCAATGCTTATGAAGATCTTTCTGTCCGTGAAAATAAAAGAGTGAGAATCCAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

30.3

Weight (kDa)

9.75

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 112, 141
AclWI GGATC 4 cut(s) 192, 502, 515, 597
AcsI RAATTY 1 cut(s) 275
AcuI CTGAAG 1 cut(s) 44
AfaI GTAC 2 cut(s) 254, 514
AfiI CCNNNNNNNGG 2 cut(s) 466, 504
AgsI TTSAA 4 cut(s) 159, 373, 545, 685
AhlI ACTAGT 1 cut(s) 14
AjnI CCWGG 1 cut(s) 615
AluBI AGCT 4 cut(s) 23, 259, 379, 455
AluI AGCT 4 cut(s) 23, 259, 379, 455
Alw26I GTCTC 4 cut(s) 26, 302, 330, 464
AlwI GGATC 4 cut(s) 192, 502, 515, 597
Ama87I CYCGRG 1 cut(s) 216
AoxI GGCC 3 cut(s) 212, 263, 664
ApeKI GCWGC 1 cut(s) 233
ApoI RAATTY 1 cut(s) 275
AspS9I GGNCC 1 cut(s) 212
AsuHPI GGTGA 2 cut(s) 535, 548
AvaI CYCGRG 1 cut(s) 216
BamHI GGATCC 1 cut(s) 507
BbsI GAAGAC 1 cut(s) 552
BbvI GCAGC 1 cut(s) 220
BccI CCATC 1 cut(s) 175
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 1 cut(s) 617
BcoDI GTCTC 4 cut(s) 26, 302, 330, 464
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 1 cut(s) 15
BglII AGATCT 1 cut(s) 736
BisI GCNGC 1 cut(s) 234
BlsI GCNGC 1 cut(s) 235
Bme1390I CCNGG 1 cut(s) 617
BmeT110I CYCGRG 1 cut(s) 216
BmgT120I GGNCC 1 cut(s) 212
BmiI GGNNCC 1 cut(s) 509
BmrFI CCNGG 1 cut(s) 617
BmsI GCATC 3 cut(s) 79, 181, 622
BpiI GAAGAC 1 cut(s) 552
BsaBI GATNNNNATC 1 cut(s) 588
BsaJI CCNNGG 1 cut(s) 215
Bsc4I CCNNNNNNNGG 2 cut(s) 466, 504
Bse1I ACTGG 1 cut(s) 69
Bse8I GATNNNNATC 1 cut(s) 588
BseBI CCWGG 1 cut(s) 617
BseDI CCNNGG 1 cut(s) 215
BseGI GGATG 2 cut(s) 369, 424
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 2 cut(s) 466, 504
BseMII CTCAG 1 cut(s) 531
BseNI ACTGG 1 cut(s) 69
BseRI GAGGAG 1 cut(s) 730
BseXI GCAGC 1 cut(s) 220
BsgI GTGCAG 1 cut(s) 698
Bsh1285I CGRYCG 1 cut(s) 293
BshFI GGCC 3 cut(s) 214, 265, 666
BsiEI CGRYCG 1 cut(s) 293
BsiHKCI CYCGRG 1 cut(s) 216
BslI CCNNNNNNNGG 2 cut(s) 466, 504
BsmAI GTCTC 4 cut(s) 26, 302, 330, 464
BsnI GGCC 3 cut(s) 214, 265, 666
BsoBI CYCGRG 1 cut(s) 216
Bsp143I GATC 4 cut(s) 184, 507, 589, 736
BspACI CCGC 2 cut(s) 112, 141
BspANI GGCC 3 cut(s) 214, 265, 666
BspCNI CTCAG 1 cut(s) 530
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 509
BspPI GGATC 4 cut(s) 192, 502, 515, 597
BsrI ACTGG 1 cut(s) 69
BssECI CCNNGG 1 cut(s) 215
BssMI GATC 4 cut(s) 184, 507, 589, 736
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 3 cut(s) 291, 629, 676
Bst6I CTCTTC 2 cut(s) 202, 707
BstDEI CTNAG 2 cut(s) 446, 517
BstF5I GGATG 2 cut(s) 369, 424
BstKTI GATC 4 cut(s) 187, 510, 592, 739
BstMAI GTCTC 4 cut(s) 26, 302, 330, 464
BstMBI GATC 4 cut(s) 184, 507, 589, 736
BstMCI CGRYCG 1 cut(s) 293
BstNI CCWGG 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 615
BstV1I GCAGC 1 cut(s) 220
BstV2I GAAGAC 1 cut(s) 552
BstX2I RGATCY 3 cut(s) 184, 507, 736
BstYI RGATCY 3 cut(s) 184, 507, 736
BsuRI GGCC 3 cut(s) 214, 265, 666
BtsCI GGATG 2 cut(s) 369, 424
BtsIMutI CAGTG 2 cut(s) 525, 625
CciI TCATGA 1 cut(s) 271
Cfr13I GGNCC 1 cut(s) 212
Csp6I GTAC 2 cut(s) 253, 513
CviAII CATG 2 cut(s) 55, 272
CviQI GTAC 2 cut(s) 253, 513
DdeI CTNAG 2 cut(s) 446, 517
DpnI GATC 4 cut(s) 186, 509, 591, 738
DpnII GATC 4 cut(s) 184, 507, 589, 736
Eam1104I CTCTTC 2 cut(s) 202, 707
EarI CTCTTC 2 cut(s) 202, 707
Eco57I CTGAAG 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 216
EcoO109I RGGNCCY 1 cut(s) 212
EcoRI GAATTC 1 cut(s) 275
EcoRII CCWGG 1 cut(s) 615
FaeI CATG 2 cut(s) 58, 275
FatI CATG 2 cut(s) 54, 271
FauI CCCGC 1 cut(s) 105
Fnu4HI GCNGC 1 cut(s) 234
FokI GGATG 2 cut(s) 376, 411
Fsp4HI GCNGC 1 cut(s) 234
FspBI CTAG 1 cut(s) 15
GluI GCNGC 1 cut(s) 234
HaeIII GGCC 3 cut(s) 214, 265, 666
Hin1II CATG 2 cut(s) 58, 275
HindIII AAGCTT 3 cut(s) 21, 257, 453
HinfI GANTC 4 cut(s) 34, 310, 373, 768
HphI GGTGA 2 cut(s) 535, 548
Hpy166II GTNNAC 1 cut(s) 74
Hpy188I TCNGA 4 cut(s) 180, 307, 461, 711
Hpy188III TCNNGA 4 cut(s) 188, 218, 272, 349
Hpy8I GTNNAC 1 cut(s) 74
HpyAV CCTTC 2 cut(s) 501, 679
HpyCH4III ACNGT 3 cut(s) 291, 629, 676
HpyCH4IV ACGT 1 cut(s) 95
HpyCH4V TGCA 2 cut(s) 285, 679
HpyF3I CTNAG 2 cut(s) 446, 517
HpySE526I ACGT 1 cut(s) 95
Hsp92II CATG 2 cut(s) 58, 275
Kzo9I GATC 4 cut(s) 184, 507, 589, 736
LpnPI CCDG 8 cut(s) 82, 143, 334, 492, 602, 629, 680, 708
Lsp1109I GCAGC 1 cut(s) 220
LweI GCATC 3 cut(s) 79, 181, 622
MaeI CTAG 1 cut(s) 15
MaeII ACGT 1 cut(s) 95
MaeIII GTNAC 1 cut(s) 655
MalI GATC 4 cut(s) 186, 509, 591, 738
MboI GATC 4 cut(s) 184, 507, 589, 736
MboII GAAGA 5 cut(s) 219, 311, 557, 724, 746
MflI RGATCY 3 cut(s) 184, 507, 736
MluCI AATT 2 cut(s) 275, 368
MlyI GAGTC 2 cut(s) 28, 304
MmeI TCCRAC 1 cut(s) 175
MnlI CCTC 6 cut(s) 203, 225, 352, 526, 708, 711
MslI CAYNNNNRTG 1 cut(s) 630
MspR9I CCNGG 1 cut(s) 617
MvaI CCWGG 1 cut(s) 617
NdeII GATC 4 cut(s) 184, 507, 589, 736
NlaIII CATG 2 cut(s) 58, 275
NlaIV GGNNCC 1 cut(s) 509
NmeAIII GCCGAG 1 cut(s) 523
PagI TCATGA 1 cut(s) 271
PfeI GAWTC 2 cut(s) 373, 768
PkrI GCNGC 1 cut(s) 235
PleI GAGTC 2 cut(s) 28, 304
PpsI GAGTC 2 cut(s) 28, 304
Psp6I CCWGG 1 cut(s) 615
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 1 cut(s) 509
PspPI GGNCC 1 cut(s) 212
PsuI RGATCY 3 cut(s) 184, 507, 736
RsaI GTAC 2 cut(s) 254, 514
RsaNI GTAC 2 cut(s) 253, 513
RseI CAYNNNNRTG 1 cut(s) 630
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 4 cut(s) 184, 507, 589, 736
Sau96I GGNCC 1 cut(s) 212
SchI GAGTC 2 cut(s) 28, 304
ScrFI CCNGG 1 cut(s) 617
SetI ASST 9 cut(s) 25, 98, 203, 261, 381, 457, 493, 518, 613
SfaNI GCATC 3 cut(s) 79, 181, 622
SmiMI CAYNNNNRTG 1 cut(s) 630
SpeI ACTAGT 1 cut(s) 14
Sse9I AATT 2 cut(s) 275, 368
SsiI CCGC 2 cut(s) 112, 141
SspMI CTAG 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 615
TaaI ACNGT 3 cut(s) 291, 629, 676
TaiI ACGT 1 cut(s) 98
TaqI TCGA 1 cut(s) 432
TasI AATT 2 cut(s) 275, 368
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 2 cut(s) 373, 768
TscAI CASTG 2 cut(s) 525, 632
TseI GCWGC 1 cut(s) 233
TspDTI ATGAA 7 cut(s) 43, 288, 311, 380, 473, 694, 747
TspGWI ACGGA 2 cut(s) 500, 737
TspRI CASTG 2 cut(s) 525, 632
XapI RAATTY 1 cut(s) 275
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.