RLG00000032180

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016316
Physical Location & Seq
Forward (+)
11841342 .. 11842565
1224 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032180

Sequence Viewer

Length: 669 bp
ATGGCCGAGTCCTCAAACAAAACCTGGCGACACCGGCGGAACGTGACTAGGCCCAGAAAAGCAAAGAAACAAACCTTACCGGCGTTATCAGAGTCAGAATCACTGCGAGGAGGCCGGAACTGGATTGACCTTCCGGACGACGTCACCGCTTCGATTCTGTCACGGCTGTCAATGTTCGACATCCTGGAGAGGGCTCAGAAGGTGTGCCTGACGTGGCGCAGAATCTGTAAGGACCCTATGACTTGGCGCGCCATCCACATGGAGTTCCATATTCCTCTGCGCCAACAAAAAATTAAAGGAAATTTCACTTCCGCCAAAAGAAAAAAGCATCTGCGCAAATTCCACAACTTCAATGTACATAAGATGTGCCACCACGCCATTGATCTTAGCTGTGGCAATCTCGTAGATATCAGTATCCGGAACTGTGGCACAGATGAGCTCCTTGAGCATATGACTGATAGACATCTTCATCTGAATAGTAAGGGATGGGGACGGCGCGCCATTCATGAAGGTTGTTCATCACATCTTGAAGATTTGACGAGAAGATCTTTTGAACAAATTACAAAACTGTGGCTTCCTGACGATTCTGTACATGACTATGAGCATCTTGCCATTGATAAAATTGAAAACAGACAACACGAAGCATATTATGAAGATATGATGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

223

Amino Acids

26.33

Weight (kDa)

9.38

Isoelectric Point (pI)

53.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 41 - 87 3.9e-09 F-box-like
F-box PF00646 41 - 83 3.4e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016316)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g26250 FvH4_5g19531
rosa_chinensis RchiOBHm_Chr5g0015241
rosa_laevigata RLG00000032180
rosa_multiflora Rmu_sc0002209.1_g000024
rosa_roxburghii Rroxscaffold_1G00061330
rosa_rugosa Rorug05G0024900
rosa_samantha Rh5AG119400 Rh5AG119500 Rh5BG117500 Rh5BG118300 Rh5CG128600 Rh5DG116600 Rh5DG116900
rosa_wichuraiana Rw5G010320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 144
Acc16I TGCGCA 1 cut(s) 335
AccII CGCG 2 cut(s) 249, 498
AccIII TCCGGA 2 cut(s) 133, 417
AciI CCGC 3 cut(s) 37, 147, 312
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 301, 338
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 2 cut(s) 357, 591
AfiI CCNNNNNNNGG 1 cut(s) 190
AgsI TTSAA 4 cut(s) 352, 530, 554, 626
AjiI CACGTC 1 cut(s) 213
AjnI CCWGG 2 cut(s) 23, 183
AluBI AGCT 2 cut(s) 390, 439
AluI AGCT 2 cut(s) 390, 439
Alw21I GWGCWC 1 cut(s) 441
AlwNI CAGNNNCTG 1 cut(s) 225
Aor13HI TCCGGA 2 cut(s) 133, 417
AoxI GGCC 3 cut(s) 3, 50, 112
ApoI RAATTY 2 cut(s) 301, 338
AscI GGCGCGCC 2 cut(s) 247, 496
AspLEI GCGC 7 cut(s) 219, 249, 251, 282, 336, 498, 500
AspS9I GGNCC 2 cut(s) 51, 232
AsuHPI GGTGA 1 cut(s) 136
AvaII GGWCC 1 cut(s) 232
BanII GRGCYC 2 cut(s) 196, 441
Bbv12I GWGCWC 1 cut(s) 441
BccI CCATC 2 cut(s) 260, 480
BceAI ACGGC 2 cut(s) 179, 509
BciT130I CCWGG 2 cut(s) 25, 185
BciVI GTATCC 1 cut(s) 425
BfaI CTAG 2 cut(s) 48, 667
BfuI GTATCC 1 cut(s) 425
BglII AGATCT 1 cut(s) 545
Bme1390I CCNGG 2 cut(s) 25, 185
Bme18I GGWCC 1 cut(s) 232
BmgBI CACGTC 1 cut(s) 213
BmgT120I GGNCC 2 cut(s) 51, 232
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 2 cut(s) 25, 185
BmsI GCATC 2 cut(s) 337, 613
BpmI CTGGAG 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 464
BsaBI GATNNNNATC 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 141
BsaWI WCCGGW 2 cut(s) 133, 417
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse118I RCCGGY 2 cut(s) 33, 79
Bse1I ACTGG 1 cut(s) 125
Bse8I GATNNNNATC 1 cut(s) 462
BseAI TCCGGA 2 cut(s) 133, 417
BseBI CCWGG 2 cut(s) 25, 185
BseGI GGATG 3 cut(s) 180, 252, 491
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 209
BseNI ACTGG 1 cut(s) 125
BsePI GCGCGC 2 cut(s) 247, 496
BseRI GAGGAG 1 cut(s) 123
Bsh1236I CGCG 2 cut(s) 249, 498
BshFI GGCC 3 cut(s) 5, 52, 114
BsiHKAI GWGCWC 1 cut(s) 441
BsiSI CCGG 5 cut(s) 34, 80, 115, 134, 418
BslFI GGGAC 1 cut(s) 504
BslI CCNNNNNNNGG 1 cut(s) 190
BsmFI GGGAC 1 cut(s) 504
BsnI GGCC 3 cut(s) 5, 52, 114
Bsp1286I GDGCHC 2 cut(s) 196, 441
Bsp13I TCCGGA 2 cut(s) 133, 417
Bsp1407I TGTACA 2 cut(s) 355, 589
Bsp143I GATC 2 cut(s) 382, 545
BspACI CCGC 3 cut(s) 37, 147, 312
BspANI GGCC 3 cut(s) 5, 52, 114
BspCNI CTCAG 1 cut(s) 208
BspEI TCCGGA 2 cut(s) 133, 417
BspFNI CGCG 2 cut(s) 249, 498
BspHI TCATGA 1 cut(s) 505
BspLI GGNNCC 1 cut(s) 234
BsrFI RCCGGY 2 cut(s) 33, 79
BsrGI TGTACA 2 cut(s) 355, 589
BsrI ACTGG 1 cut(s) 125
BssAI RCCGGY 2 cut(s) 33, 79
BssHII GCGCGC 2 cut(s) 247, 496
BssMI GATC 2 cut(s) 382, 545
BssNI GRCGYC 1 cut(s) 141
Bst2UI CCWGG 2 cut(s) 25, 185
Bst4CI ACNGT 2 cut(s) 425, 570
BstACI GRCGYC 1 cut(s) 141
BstAUI TGTACA 2 cut(s) 355, 589
BstC8I GCNNGC 2 cut(s) 249, 498
BstDEI CTNAG 2 cut(s) 195, 386
BstF5I GGATG 3 cut(s) 180, 252, 491
BstFNI CGCG 2 cut(s) 249, 498
BstHHI GCGC 7 cut(s) 219, 249, 251, 282, 336, 498, 500
BstKTI GATC 2 cut(s) 385, 548
BstMBI GATC 2 cut(s) 382, 545
BstMWI GCNNNNNNNGC 2 cut(s) 34, 445
BstNI CCWGG 2 cut(s) 25, 185
BstSCI CCNGG 2 cut(s) 23, 183
BstUI CGCG 2 cut(s) 249, 498
BstX2I RGATCY 1 cut(s) 545
BstXI CCANNNNNNTGG 1 cut(s) 259
BstYI RGATCY 1 cut(s) 545
BsuI GTATCC 1 cut(s) 425
BsuRI GGCC 3 cut(s) 5, 52, 114
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 3 cut(s) 180, 252, 491
BtsI GCAGTG 1 cut(s) 101
BtsIMutI CAGTG 1 cut(s) 101
Cac8I GCNNGC 2 cut(s) 249, 498
CaiI CAGNNNCTG 1 cut(s) 225
CciI TCATGA 1 cut(s) 505
CfoI GCGC 7 cut(s) 219, 249, 251, 282, 336, 498, 500
Cfr10I RCCGGY 2 cut(s) 33, 79
Cfr13I GGNCC 2 cut(s) 51, 232
Csp6I GTAC 2 cut(s) 356, 590
CviAII CATG 3 cut(s) 259, 506, 593
CviJI RGCY 8 cut(s) 5, 52, 114, 166, 194, 390, 439, 574
CviKI_1 RGCY 8 cut(s) 5, 52, 114, 166, 194, 390, 439, 574
CviQI GTAC 2 cut(s) 356, 590
DdeI CTNAG 2 cut(s) 195, 386
DpnI GATC 2 cut(s) 384, 547
DpnII GATC 2 cut(s) 382, 545
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 2 cut(s) 52, 301
Ecl136II GAGCTC 1 cut(s) 439
Eco24I GRGCYC 2 cut(s) 196, 441
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 439
EcoICRI GAGCTC 1 cut(s) 439
EcoO109I RGGNCCY 1 cut(s) 232
EcoRII CCWGG 2 cut(s) 23, 183
EcoRV GATATC 1 cut(s) 409
EcoT38I GRGCYC 2 cut(s) 196, 441
FaeI CATG 3 cut(s) 262, 509, 596
FaqI GGGAC 1 cut(s) 504
FatI CATG 3 cut(s) 258, 505, 592
FauNDI CATATG 1 cut(s) 450
FokI GGATG 3 cut(s) 167, 239, 498
FriOI GRGCYC 2 cut(s) 196, 441
FspBI CTAG 2 cut(s) 48, 667
FspI TGCGCA 1 cut(s) 335
GlaI GCGC 7 cut(s) 218, 248, 250, 281, 335, 497, 499
GsuI CTGGAG 1 cut(s) 206
HaeIII GGCC 3 cut(s) 5, 52, 114
HapII CCGG 5 cut(s) 34, 80, 115, 134, 418
HhaI GCGC 7 cut(s) 219, 249, 251, 282, 336, 498, 500
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 3 cut(s) 262, 509, 596
Hin6I GCGC 7 cut(s) 217, 247, 249, 280, 334, 496, 498
HinP1I GCGC 7 cut(s) 217, 247, 249, 280, 334, 496, 498
HinfI GANTC 6 cut(s) 8, 92, 98, 154, 222, 584
HpaII CCGG 5 cut(s) 34, 80, 115, 134, 418
HphI GGTGA 1 cut(s) 136
Hpy188I TCNGA 4 cut(s) 91, 97, 198, 474
Hpy188III TCNNGA 5 cut(s) 134, 418, 506, 527, 578
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 3 cut(s) 140, 193, 503
HpyCH4III ACNGT 2 cut(s) 425, 570
HpyCH4IV ACGT 3 cut(s) 42, 141, 212
HpyF10VI GCNNNNNNNGC 2 cut(s) 34, 445
HpyF3I CTNAG 2 cut(s) 195, 386
HpySE526I ACGT 3 cut(s) 42, 141, 212
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 3 cut(s) 262, 509, 596
HspAI GCGC 7 cut(s) 217, 247, 249, 280, 334, 496, 498
Kpn2I TCCGGA 2 cut(s) 133, 417
Kzo9I GATC 2 cut(s) 382, 545
LmnI GCTCC 1 cut(s) 444
LweI GCATC 2 cut(s) 337, 613
MaeI CTAG 2 cut(s) 48, 667
MaeII ACGT 3 cut(s) 42, 141, 212
MaeIII GTNAC 3 cut(s) 43, 142, 159
MalI GATC 2 cut(s) 384, 547
MboI GATC 2 cut(s) 382, 545
MboII GAAGA 4 cut(s) 458, 542, 555, 665
MflI RGATCY 1 cut(s) 545
MhlI GDGCHC 2 cut(s) 196, 441
MluCI AATT 5 cut(s) 291, 301, 338, 558, 621
MlyI GAGTC 2 cut(s) 17, 101
MnlI CCTC 5 cut(s) 22, 101, 104, 183, 285
MroI TCCGGA 2 cut(s) 133, 417
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 2 cut(s) 257, 597
MspI CCGG 5 cut(s) 34, 80, 115, 134, 418
MspR9I CCNGG 2 cut(s) 25, 185
MvaI CCWGG 2 cut(s) 25, 185
MvnI CGCG 2 cut(s) 249, 498
MwoI GCNNNNNNNGC 2 cut(s) 34, 445
NdeI CATATG 1 cut(s) 450
NdeII GATC 2 cut(s) 382, 545
NlaIII CATG 3 cut(s) 262, 509, 596
NlaIV GGNNCC 1 cut(s) 234
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 3 cut(s) 43, 142, 159
NsbI TGCGCA 1 cut(s) 335
PagI TCATGA 1 cut(s) 505
PalAI GGCGCGCC 2 cut(s) 247, 496
PauI GCGCGC 2 cut(s) 247, 496
PfeI GAWTC 4 cut(s) 98, 154, 222, 584
PflFI GACNNNGTC 1 cut(s) 140
PfoI TCCNGGA 1 cut(s) 183
PleI GAGTC 2 cut(s) 16, 100
PpsI GAGTC 2 cut(s) 16, 100
PpuMI RGGWCCY 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 441
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 2 cut(s) 23, 183
PspGI CCWGG 2 cut(s) 23, 183
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 2 cut(s) 51, 232
PspPPI RGGWCCY 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 225
PsuI RGATCY 1 cut(s) 545
PsyI GACNNNGTC 1 cut(s) 140
PteI GCGCGC 2 cut(s) 247, 496
RsaI GTAC 2 cut(s) 357, 591
RsaNI GTAC 2 cut(s) 356, 590
RseI CAYNNNNRTG 2 cut(s) 257, 597
SacI GAGCTC 1 cut(s) 441
SaqAI TTAA 1 cut(s) 294
Sau3AI GATC 2 cut(s) 382, 545
Sau96I GGNCC 2 cut(s) 51, 232
SchI GAGTC 2 cut(s) 17, 101
ScrFI CCNGG 2 cut(s) 25, 185
SduI GDGCHC 2 cut(s) 196, 441
SfaNI GCATC 2 cut(s) 337, 613
SgrAI CRCCGGYG 1 cut(s) 33
SgsI GGCGCGCC 2 cut(s) 247, 496
SinI GGWCC 1 cut(s) 232
SmiMI CAYNNNNRTG 2 cut(s) 257, 597
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 5 cut(s) 291, 301, 338, 558, 621
SsiI CCGC 3 cut(s) 37, 147, 312
SspMI CTAG 2 cut(s) 48, 667
SstI GAGCTC 1 cut(s) 441
StyD4I CCNGG 2 cut(s) 23, 183
TaaI ACNGT 2 cut(s) 425, 570
TaiI ACGT 3 cut(s) 45, 144, 215
TaqI TCGA 2 cut(s) 152, 177
TasI AATT 5 cut(s) 291, 301, 338, 558, 621
TatI WGTACW 2 cut(s) 355, 589
TfiI GAWTC 4 cut(s) 98, 154, 222, 584
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 1 cut(s) 108
TseFI GTSAC 3 cut(s) 43, 142, 159
Tsp45I GTSAC 3 cut(s) 43, 142, 159
TspDTI ATGAA 5 cut(s) 458, 494, 507, 522, 666
TspRI CASTG 1 cut(s) 108
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 2 cut(s) 301, 338
XspI CTAG 2 cut(s) 48, 667
ZraI GACGTC 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.