Rroxscaffold_1G00061330

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016316
Physical Location & Seq
Reverse (-)
83493439 .. 83494665
1227 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00061330.1

Sequence Viewer

Length: 1056 bp
ATGGCCGAGTCCTCAAACAAAACCTGGCGACGCCGGCGGAACGTGACTAGTCCCAGAAAAGCAAAGAAACAGACCCTACCGGCGTTATCAGAGTCAGAATCACTGCGAGGAGGCCGGAACTGGATTGACCTTCCGGACGACATCACCGCTTCGATTCTGTCACGGCTGTCAATGTTCGACATCCTGGAGAGGGCTCAGAAGGTGTGCCTGACGTGGCGCAGAATCTGTAAGGACCCTATGACTTGGCGCGCCATCCACATGGAGTTCCATATTCATCTGCGCCAACAAAAACTTGAAGGAAATTTCACTTCCGCCAAAAGAAAAAAGCATCTGCGCAAATTCCACAACTTCAATGTACATAAGATGTGCCACCACGCCATTGATCTTAGCTGTGGCAATCTCGTAGATATCAGTATCCGGAACTGTGGCACAGATGAGCTCCTTGAGCATATGACTGATAGTTCGAGGGGAATCAGACGCCTCAGTATTGTGTTTTCAGCTTACATAACAATGAAGGGACTAAGTAAAGTGGCTTCGAAGCTTCCGATGTTGGAGGATCTTGCAATCTCATACACCCCACGGATTAGTAAAAGTTTCGGAGTCTTACACAGTTGTGAATATCTTGAAGTGATTGGCCGTTCTTGCCCTCTTTTGAAATCTTTGAAATTGAACAAGCACCCCGGGGATATGTTTAACAGCAAAGACAATGGTGATGCATTTGCGATAGCCATAACAATGCATGGGTTAAAGAAGCTCCAGCTTTTTGGAAATTGGATGAATCATCAGGGATTGCACGCCATTCTTGTAGGTTGTCCGCATCTTGAGTCACTTGATCTCCGTCAATGTACACATCTTCATCTGAATAGTAAGGGATGGGGATGGCGAGCCATTCATGAAGGTTGTTCATCACATCTTCAAGATTTGACAACAAGATGTTTTGAACAAATTACAAAACTGTGGCTTCCTGACGATTCTGTACATGACTATGAGCATCTTGCCATTGATAAAATTGAAAACAGACAATACGAAGCGTATACTGAAGATATGATGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

351

Amino Acids

40.51

Weight (kDa)

9.2

Isoelectric Point (pI)

48.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 41 - 87 6.5e-09 F-box-like
F-box PF00646 41 - 83 6.5e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016316)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g26250 FvH4_5g19531
rosa_chinensis RchiOBHm_Chr5g0015241
rosa_laevigata RLG00000032180
rosa_multiflora Rmu_sc0002209.1_g000024
rosa_roxburghii Rroxscaffold_1G00061330
rosa_rugosa Rorug05G0024900
rosa_samantha Rh5AG119400 Rh5AG119500 Rh5BG117500 Rh5BG118300 Rh5CG128600 Rh5DG116600 Rh5DG116900
rosa_wichuraiana Rw5G010320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 335
AccI GTMKAC 1 cut(s) 1034
AccII CGCG 1 cut(s) 249
AccIII TCCGGA 2 cut(s) 133, 417
AciI CCGC 4 cut(s) 37, 147, 312, 815
AclWI GGATC 1 cut(s) 564
AcoI YGGCCR 2 cut(s) 3, 634
AcsI RAATTY 2 cut(s) 301, 338
AcyI GRCGYC 2 cut(s) 31, 478
AfaI GTAC 3 cut(s) 357, 847, 978
AfiI CCNNNNNNNGG 1 cut(s) 190
AgsI TTSAA 9 cut(s) 296, 352, 626, 655, 664, 670, 917, 941, 1013
AhlI ACTAGT 1 cut(s) 47
AjiI CACGTC 1 cut(s) 213
AjnI CCWGG 2 cut(s) 23, 183
AleI CACNNNNGTG 1 cut(s) 612
AluBI AGCT 6 cut(s) 390, 439, 500, 541, 754, 760
AluI AGCT 6 cut(s) 390, 439, 500, 541, 754, 760
Alw21I GWGCWC 1 cut(s) 441
AlwI GGATC 1 cut(s) 564
AlwNI CAGNNNCTG 1 cut(s) 225
Ama87I CYCGRG 1 cut(s) 680
Aor13HI TCCGGA 2 cut(s) 133, 417
AoxI GGCC 3 cut(s) 3, 112, 634
ApoI RAATTY 2 cut(s) 301, 338
ArsI GACNNNNNNTTYG 2 cut(s) 445, 477
AscI GGCGCGCC 1 cut(s) 247
AspLEI GCGC 5 cut(s) 219, 249, 251, 282, 336
AspS9I GGNCC 1 cut(s) 232
AsuC2I CCSGG 2 cut(s) 681, 682
AsuHPI GGTGA 2 cut(s) 136, 722
AsuII TTCGAA 1 cut(s) 536
AvaI CYCGRG 1 cut(s) 680
AvaII GGWCC 1 cut(s) 232
BanII GRGCYC 2 cut(s) 196, 441
BarI GAAGNNNNNNTAC 2 cut(s) 517, 549
Bbv12I GWGCWC 1 cut(s) 441
BccI CCATC 3 cut(s) 260, 867, 873
BceAI ACGGC 2 cut(s) 179, 621
BciT130I CCWGG 2 cut(s) 25, 185
BciVI GTATCC 1 cut(s) 425
BcnI CCSGG 2 cut(s) 681, 682
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 2 cut(s) 48, 1054
BfuI GTATCC 1 cut(s) 425
Bme1390I CCNGG 4 cut(s) 25, 185, 681, 682
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 680
BmgBI CACGTC 1 cut(s) 213
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 4 cut(s) 25, 185, 681, 682
BmsI GCATC 4 cut(s) 337, 703, 826, 1000
BpmI CTGGAG 2 cut(s) 206, 740
Bpu14I TTCGAA 1 cut(s) 536
BpuEI CTTGAG 2 cut(s) 464, 842
BpuMI CCSGG 2 cut(s) 681, 682
BsaHI GRCGYC 2 cut(s) 31, 478
BsaJI CCNNGG 4 cut(s) 578, 679, 680, 681
BsaWI WCCGGW 2 cut(s) 133, 417
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse118I RCCGGY 2 cut(s) 33, 79
Bse1I ACTGG 1 cut(s) 125
BseAI TCCGGA 2 cut(s) 133, 417
BseBI CCWGG 2 cut(s) 25, 185
BseDI CCNNGG 4 cut(s) 578, 679, 680, 681
BseGI GGATG 5 cut(s) 180, 252, 780, 878, 884
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 2 cut(s) 209, 496
BseNI ACTGG 1 cut(s) 125
BsePI GCGCGC 1 cut(s) 247
BseRI GAGGAG 1 cut(s) 123
Bsh1236I CGCG 1 cut(s) 249
BshFI GGCC 3 cut(s) 5, 114, 636
BsiHKAI GWGCWC 1 cut(s) 441
BsiHKCI CYCGRG 1 cut(s) 680
BsiSI CCGG 6 cut(s) 34, 80, 115, 134, 418, 681
BslFI GGGAC 2 cut(s) 36, 531
BslI CCNNNNNNNGG 1 cut(s) 190
BsmFI GGGAC 2 cut(s) 36, 531
BsnI GGCC 3 cut(s) 5, 114, 636
BsoBI CYCGRG 1 cut(s) 680
Bsp119I TTCGAA 1 cut(s) 536
Bsp1286I GDGCHC 2 cut(s) 196, 441
Bsp13I TCCGGA 2 cut(s) 133, 417
Bsp1407I TGTACA 3 cut(s) 355, 845, 976
Bsp143I GATC 4 cut(s) 382, 556, 832, 1050
BspACI CCGC 4 cut(s) 37, 147, 312, 815
BspANI GGCC 3 cut(s) 5, 114, 636
BspCNI CTCAG 2 cut(s) 208, 495
BspEI TCCGGA 2 cut(s) 133, 417
BspFNI CGCG 1 cut(s) 249
BspHI TCATGA 1 cut(s) 892
BspLI GGNNCC 1 cut(s) 234
BspPI GGATC 1 cut(s) 564
BspT104I TTCGAA 1 cut(s) 536
BsrFI RCCGGY 2 cut(s) 33, 79
BsrGI TGTACA 3 cut(s) 355, 845, 976
BsrI ACTGG 1 cut(s) 125
BssAI RCCGGY 2 cut(s) 33, 79
BssECI CCNNGG 4 cut(s) 578, 679, 680, 681
BssHII GCGCGC 1 cut(s) 247
BssMI GATC 4 cut(s) 382, 556, 832, 1050
BssNAI GTATAC 1 cut(s) 1035
BssNI GRCGYC 2 cut(s) 31, 478
Bst1107I GTATAC 1 cut(s) 1035
Bst2UI CCWGG 2 cut(s) 25, 185
Bst4CI ACNGT 3 cut(s) 425, 611, 957
BstACI GRCGYC 2 cut(s) 31, 478
BstAUI TGTACA 3 cut(s) 355, 845, 976
BstBI TTCGAA 1 cut(s) 536
BstC8I GCNNGC 4 cut(s) 35, 249, 795, 885
BstDEI CTNAG 4 cut(s) 195, 386, 482, 521
BstDSI CCRYGG 1 cut(s) 578
BstF5I GGATG 5 cut(s) 180, 252, 780, 878, 884
BstFNI CGCG 1 cut(s) 249
BstHHI GCGC 5 cut(s) 219, 249, 251, 282, 336
BstKTI GATC 4 cut(s) 385, 559, 835, 1053
BstMBI GATC 4 cut(s) 382, 556, 832, 1050
BstMWI GCNNNNNNNGC 3 cut(s) 34, 445, 642
BstNI CCWGG 2 cut(s) 25, 185
BstSCI CCNGG 4 cut(s) 23, 183, 679, 680
BstUI CGCG 1 cut(s) 249
BstX2I RGATCY 1 cut(s) 556
BstXI CCANNNNNNTGG 2 cut(s) 259, 764
BstYI RGATCY 1 cut(s) 556
BstZ17I GTATAC 1 cut(s) 1035
BsuI GTATCC 1 cut(s) 425
BsuRI GGCC 3 cut(s) 5, 114, 636
BtgI CCRYGG 1 cut(s) 578
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 5 cut(s) 180, 252, 780, 878, 884
BtsI GCAGTG 1 cut(s) 101
BtsIMutI CAGTG 1 cut(s) 101
Cac8I GCNNGC 4 cut(s) 35, 249, 795, 885
CaiI CAGNNNCTG 1 cut(s) 225
CciI TCATGA 1 cut(s) 892
CfoI GCGC 5 cut(s) 219, 249, 251, 282, 336
Cfr10I RCCGGY 2 cut(s) 33, 79
Cfr13I GGNCC 1 cut(s) 232
Cfr9I CCCGGG 1 cut(s) 680
CseI GACGC 2 cut(s) 39, 486
Csp6I GTAC 3 cut(s) 356, 846, 977
CviAII CATG 4 cut(s) 259, 740, 893, 980
CviQI GTAC 3 cut(s) 356, 846, 977
DdeI CTNAG 4 cut(s) 195, 386, 482, 521
DpnI GATC 4 cut(s) 384, 558, 834, 1052
DpnII GATC 4 cut(s) 382, 556, 832, 1050
EaeI YGGCCR 2 cut(s) 3, 634
EciI GGCGGA 2 cut(s) 52, 301
Ecl136II GAGCTC 1 cut(s) 439
Eco24I GRGCYC 2 cut(s) 196, 441
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 439
Eco88I CYCGRG 1 cut(s) 680
EcoICRI GAGCTC 1 cut(s) 439
EcoO109I RGGNCCY 1 cut(s) 232
EcoRII CCWGG 2 cut(s) 23, 183
EcoRV GATATC 1 cut(s) 409
EcoT22I ATGCAT 2 cut(s) 718, 741
EcoT38I GRGCYC 2 cut(s) 196, 441
FaeI CATG 4 cut(s) 262, 743, 896, 983
FaqI GGGAC 2 cut(s) 36, 531
FatI CATG 4 cut(s) 258, 739, 892, 979
FauNDI CATATG 1 cut(s) 450
FblI GTMKAC 1 cut(s) 1034
FokI GGATG 5 cut(s) 167, 239, 787, 885, 891
FriOI GRGCYC 2 cut(s) 196, 441
FspBI CTAG 2 cut(s) 48, 1054
FspI TGCGCA 1 cut(s) 335
GlaI GCGC 5 cut(s) 218, 248, 250, 281, 335
GsuI CTGGAG 2 cut(s) 206, 740
HaeIII GGCC 3 cut(s) 5, 114, 636
HapII CCGG 6 cut(s) 34, 80, 115, 134, 418, 681
HgaI GACGC 2 cut(s) 39, 486
HhaI GCGC 5 cut(s) 219, 249, 251, 282, 336
Hin1I GRCGYC 2 cut(s) 31, 478
Hin1II CATG 4 cut(s) 262, 743, 896, 983
Hin6I GCGC 5 cut(s) 217, 247, 249, 280, 334
HinP1I GCGC 5 cut(s) 217, 247, 249, 280, 334
HindIII AAGCTT 1 cut(s) 539
HpaII CCGG 6 cut(s) 34, 80, 115, 134, 418, 681
HphI GGTGA 2 cut(s) 136, 722
Hpy166II GTNNAC 2 cut(s) 848, 1035
Hpy188I TCNGA 7 cut(s) 91, 97, 198, 476, 546, 599, 861
Hpy188III TCNNGA 7 cut(s) 134, 418, 623, 821, 893, 917, 965
Hpy8I GTNNAC 2 cut(s) 848, 1035
Hpy99I CGWCG 1 cut(s) 33
HpyAV CCTTC 5 cut(s) 140, 193, 290, 508, 890
HpyCH4III ACNGT 3 cut(s) 425, 611, 957
HpyCH4IV ACGT 2 cut(s) 42, 212
HpyCH4V TGCA 4 cut(s) 563, 716, 739, 793
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 445, 642
HpyF3I CTNAG 4 cut(s) 195, 386, 482, 521
HpySE526I ACGT 2 cut(s) 42, 212
Hsp92I GRCGYC 2 cut(s) 31, 478
Hsp92II CATG 4 cut(s) 262, 743, 896, 983
HspAI GCGC 5 cut(s) 217, 247, 249, 280, 334
Kpn2I TCCGGA 2 cut(s) 133, 417
KroI GCCGGC 1 cut(s) 33
KroNI GCCGGC 1 cut(s) 35
Kzo9I GATC 4 cut(s) 382, 556, 832, 1050
LmnI GCTCC 2 cut(s) 444, 759
LweI GCATC 4 cut(s) 337, 703, 826, 1000
MaeI CTAG 2 cut(s) 48, 1054
MaeII ACGT 2 cut(s) 42, 212
MaeIII GTNAC 3 cut(s) 43, 159, 825
MalI GATC 4 cut(s) 384, 558, 834, 1052
MboI GATC 4 cut(s) 382, 556, 832, 1050
MboII GAAGA 3 cut(s) 845, 905, 1052
MflI RGATCY 1 cut(s) 556
MhlI GDGCHC 2 cut(s) 196, 441
MluCI AATT 6 cut(s) 301, 338, 665, 769, 945, 1008
MlyI GAGTC 4 cut(s) 17, 101, 609, 833
MmeI TCCRAC 1 cut(s) 531
MnlI CCTC 8 cut(s) 22, 101, 104, 183, 459, 491, 547, 657
Mph1103I ATGCAT 2 cut(s) 718, 741
MreI CGCCGGCG 1 cut(s) 33
MroI TCCGGA 2 cut(s) 133, 417
MroNI GCCGGC 1 cut(s) 33
MseI TTAA 2 cut(s) 693, 746
MslI CAYNNNNRTG 5 cut(s) 257, 509, 612, 734, 984
MspI CCGG 6 cut(s) 34, 80, 115, 134, 418, 681
MspR9I CCNGG 4 cut(s) 25, 185, 681, 682
MvaI CCWGG 2 cut(s) 25, 185
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 3 cut(s) 34, 445, 642
NaeI GCCGGC 1 cut(s) 35
NciI CCSGG 2 cut(s) 681, 682
NdeI CATATG 1 cut(s) 450
NdeII GATC 4 cut(s) 382, 556, 832, 1050
NgoMIV GCCGGC 1 cut(s) 33
NlaIII CATG 4 cut(s) 262, 743, 896, 983
NlaIV GGNNCC 1 cut(s) 234
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 3 cut(s) 43, 159, 825
NsbI TGCGCA 1 cut(s) 335
NsiI ATGCAT 2 cut(s) 718, 741
NspV TTCGAA 1 cut(s) 536
OliI CACNNNNGTG 1 cut(s) 612
PagI TCATGA 1 cut(s) 892
PalAI GGCGCGCC 1 cut(s) 247
PauI GCGCGC 1 cut(s) 247
PcsI WCGNNNNNNNCGW 1 cut(s) 542
PdiI GCCGGC 1 cut(s) 35
PfeI GAWTC 6 cut(s) 98, 154, 222, 471, 778, 971
PfoI TCCNGGA 1 cut(s) 183
PleI GAGTC 4 cut(s) 16, 100, 608, 832
PpsI GAGTC 4 cut(s) 16, 100, 608, 832
PpuMI RGGWCCY 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 441
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 2 cut(s) 23, 183
PspGI CCWGG 2 cut(s) 23, 183
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 1 cut(s) 232
PspPPI RGGWCCY 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 225
PsuI RGATCY 1 cut(s) 556
PteI GCGCGC 1 cut(s) 247
RsaI GTAC 3 cut(s) 357, 847, 978
RsaNI GTAC 3 cut(s) 356, 846, 977
RseI CAYNNNNRTG 5 cut(s) 257, 509, 612, 734, 984
SacI GAGCTC 1 cut(s) 441
SaqAI TTAA 2 cut(s) 693, 746
Sau3AI GATC 4 cut(s) 382, 556, 832, 1050
Sau96I GGNCC 1 cut(s) 232
SchI GAGTC 4 cut(s) 17, 101, 609, 833
ScrFI CCNGG 4 cut(s) 25, 185, 681, 682
SduI GDGCHC 2 cut(s) 196, 441
SfaNI GCATC 4 cut(s) 337, 703, 826, 1000
SfuI TTCGAA 1 cut(s) 536
SgrAI CRCCGGYG 1 cut(s) 33
SgsI GGCGCGCC 1 cut(s) 247
SinI GGWCC 1 cut(s) 232
SmaI CCCGGG 1 cut(s) 682
SmiMI CAYNNNNRTG 5 cut(s) 257, 509, 612, 734, 984
SmlI CTYRAG 2 cut(s) 443, 821
SmoI CTYRAG 2 cut(s) 443, 821
SpeI ACTAGT 1 cut(s) 47
Sse9I AATT 6 cut(s) 301, 338, 665, 769, 945, 1008
SsiI CCGC 4 cut(s) 37, 147, 312, 815
SspMI CTAG 2 cut(s) 48, 1054
SstI GAGCTC 1 cut(s) 441
StyD4I CCNGG 4 cut(s) 23, 183, 679, 680
TaaI ACNGT 3 cut(s) 425, 611, 957
TaiI ACGT 2 cut(s) 45, 215
TaqI TCGA 4 cut(s) 152, 177, 464, 536
TasI AATT 6 cut(s) 301, 338, 665, 769, 945, 1008
TatI WGTACW 3 cut(s) 355, 845, 976
TfiI GAWTC 6 cut(s) 98, 154, 222, 471, 778, 971
Tru1I TTAA 2 cut(s) 693, 746
Tru9I TTAA 2 cut(s) 693, 746
TscAI CASTG 1 cut(s) 108
TseFI GTSAC 3 cut(s) 43, 159, 825
Tsp45I GTSAC 3 cut(s) 43, 159, 825
TspDTI ATGAA 7 cut(s) 263, 527, 791, 845, 881, 894, 909
TspGWI ACGGA 2 cut(s) 595, 827
TspMI CCCGGG 1 cut(s) 680
TspRI CASTG 1 cut(s) 108
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 2 cut(s) 301, 338
XmaI CCCGGG 1 cut(s) 680
XmiI GTMKAC 1 cut(s) 1034
XspI CTAG 2 cut(s) 48, 1054
Zsp2I ATGCAT 2 cut(s) 718, 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.