Rh5AG119400

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0016316
Physical Location & Seq
Forward (+)
11092432 .. 11102244
9813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG119400.1

Sequence Viewer

Length: 588 bp
ATGGCCGAGTCCTCAAACAAAACCTGGCGACGCCGGCGGAACGTGACTAGGCCTAGAAAAGCAAAAAAACAAACCCTACCGGCGTTATCAGAGTCAGAATCACTGCGAGGACGCCCGAACTGGATTGACCTTCCGGACGACGTCACCGCTTCGATTCTTTCACGGCTGTCAATGTTCGACATCCTGGAGAGGGCTCAGAAGGTGTGCCTGACGTGGCGCAGAATCTGTAAGGACCCTATGACTTGGCGCGCCATCCACATGGAGTTCCATATTCATCTGCGTCAACAAAAAATTAAAGGAAATTTCACTTCCACCAAAAGAAAAAAGCATCTGCGCAAATTCCACAACTTCAATGTACATAAGATGTGCCACCACGCCATTGATCTTAGCTGTGGCAATCTCGTAGATATCAGTATCCGGAACTGTGGCACAGATGAGCTCCTTGAGCATATGACTGATAGAGTGTTAGTAATTGATCAAGAAGCTGCCTTAACTGAAGGAGCATTCCGTTGTCGCAGATCTGTTCAAAGATGGGCTGCTGCACCAGATCCTTTCTTCTTTGACTCATCAGGTGCATCATATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

195

Amino Acids

22.82

Weight (kDa)

10.08

Isoelectric Point (pI)

59.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 41 - 87 3.1e-09 F-box-like
F-box PF00646 41 - 83 2.7e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016316)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g26250 FvH4_5g19531
rosa_chinensis RchiOBHm_Chr5g0015241
rosa_laevigata RLG00000032180
rosa_multiflora Rmu_sc0002209.1_g000024
rosa_roxburghii Rroxscaffold_1G00061330
rosa_rugosa Rorug05G0024900
rosa_samantha Rh5AG119400 Rh5AG119500 Rh5BG117500 Rh5BG118300 Rh5CG128600 Rh5DG116600 Rh5DG116900
rosa_wichuraiana Rw5G010320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 144
Acc16I TGCGCA 1 cut(s) 335
AccII CGCG 1 cut(s) 249
AccIII TCCGGA 2 cut(s) 133, 417
AciI CCGC 2 cut(s) 37, 147
AclWI GGATC 1 cut(s) 542
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 301, 338
AcuI CTGAAG 1 cut(s) 516
AcyI GRCGYC 3 cut(s) 31, 112, 141
AfaI GTAC 1 cut(s) 357
AfiI CCNNNNNNNGG 1 cut(s) 190
AgsI TTSAA 2 cut(s) 352, 527
AjiI CACGTC 1 cut(s) 213
AjnI CCWGG 2 cut(s) 23, 183
AluBI AGCT 3 cut(s) 390, 439, 485
AluI AGCT 3 cut(s) 390, 439, 485
Alw21I GWGCWC 1 cut(s) 441
AlwI GGATC 1 cut(s) 542
AlwNI CAGNNNCTG 1 cut(s) 225
Aor13HI TCCGGA 2 cut(s) 133, 417
AoxI GGCC 2 cut(s) 3, 50
ApeKI GCWGC 3 cut(s) 485, 536, 539
ApoI RAATTY 2 cut(s) 301, 338
AscI GGCGCGCC 1 cut(s) 247
AspLEI GCGC 4 cut(s) 219, 249, 251, 336
AspS9I GGNCC 1 cut(s) 232
AsuHPI GGTGA 1 cut(s) 136
AvaII GGWCC 1 cut(s) 232
BanII GRGCYC 2 cut(s) 196, 441
Bbv12I GWGCWC 1 cut(s) 441
BbvI GCAGC 3 cut(s) 472, 523, 526
BccI CCATC 2 cut(s) 260, 525
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 2 cut(s) 25, 185
BciVI GTATCC 1 cut(s) 425
BclI TGATCA 1 cut(s) 475
BfaI CTAG 2 cut(s) 48, 54
BfuI GTATCC 1 cut(s) 425
BglII AGATCT 1 cut(s) 518
BisI GCNGC 3 cut(s) 486, 537, 540
BlsI GCNGC 3 cut(s) 487, 538, 541
Bme1390I CCNGG 2 cut(s) 25, 185
Bme18I GGWCC 1 cut(s) 232
BmgBI CACGTC 1 cut(s) 213
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 2 cut(s) 25, 185
BmsI GCATC 2 cut(s) 337, 584
BpmI CTGGAG 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 464
BsaHI GRCGYC 3 cut(s) 31, 112, 141
BsaWI WCCGGW 2 cut(s) 133, 417
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse118I RCCGGY 2 cut(s) 33, 79
Bse1I ACTGG 1 cut(s) 125
BseAI TCCGGA 2 cut(s) 133, 417
BseBI CCWGG 2 cut(s) 25, 185
BseGI GGATG 2 cut(s) 180, 252
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 209
BseNI ACTGG 1 cut(s) 125
BsePI GCGCGC 1 cut(s) 247
BseXI GCAGC 3 cut(s) 472, 523, 526
BsgI GTGCAG 1 cut(s) 525
Bsh1236I CGCG 1 cut(s) 249
BshFI GGCC 2 cut(s) 5, 52
BsiHKAI GWGCWC 1 cut(s) 441
BsiSI CCGG 4 cut(s) 34, 80, 134, 418
BslI CCNNNNNNNGG 1 cut(s) 190
BsmI GAATGC 1 cut(s) 503
BsnI GGCC 2 cut(s) 5, 52
Bsp1286I GDGCHC 2 cut(s) 196, 441
Bsp13I TCCGGA 2 cut(s) 133, 417
Bsp1407I TGTACA 1 cut(s) 355
Bsp143I GATC 4 cut(s) 382, 475, 518, 547
BspACI CCGC 2 cut(s) 37, 147
BspANI GGCC 2 cut(s) 5, 52
BspCNI CTCAG 1 cut(s) 208
BspEI TCCGGA 2 cut(s) 133, 417
BspFNI CGCG 1 cut(s) 249
BspLI GGNNCC 1 cut(s) 234
BspPI GGATC 1 cut(s) 542
BsrFI RCCGGY 2 cut(s) 33, 79
BsrGI TGTACA 1 cut(s) 355
BsrI ACTGG 1 cut(s) 125
BssAI RCCGGY 2 cut(s) 33, 79
BssHII GCGCGC 1 cut(s) 247
BssMI GATC 4 cut(s) 382, 475, 518, 547
BssNI GRCGYC 3 cut(s) 31, 112, 141
Bst2UI CCWGG 2 cut(s) 25, 185
Bst4CI ACNGT 1 cut(s) 425
BstACI GRCGYC 3 cut(s) 31, 112, 141
BstAUI TGTACA 1 cut(s) 355
BstC8I GCNNGC 2 cut(s) 35, 249
BstDEI CTNAG 2 cut(s) 195, 386
BstF5I GGATG 2 cut(s) 180, 252
BstFNI CGCG 1 cut(s) 249
BstHHI GCGC 4 cut(s) 219, 249, 251, 336
BstKTI GATC 4 cut(s) 385, 478, 521, 550
BstMBI GATC 4 cut(s) 382, 475, 518, 547
BstMWI GCNNNNNNNGC 2 cut(s) 34, 445
BstNI CCWGG 2 cut(s) 25, 185
BstSCI CCNGG 2 cut(s) 23, 183
BstUI CGCG 1 cut(s) 249
BstV1I GCAGC 3 cut(s) 472, 523, 526
BstX2I RGATCY 2 cut(s) 518, 547
BstXI CCANNNNNNTGG 1 cut(s) 259
BstYI RGATCY 2 cut(s) 518, 547
BsuI GTATCC 1 cut(s) 425
BsuRI GGCC 2 cut(s) 5, 52
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 2 cut(s) 180, 252
BtsI GCAGTG 1 cut(s) 101
BtsIMutI CAGTG 1 cut(s) 101
Cac8I GCNNGC 2 cut(s) 35, 249
CaiI CAGNNNCTG 1 cut(s) 225
CfoI GCGC 4 cut(s) 219, 249, 251, 336
Cfr10I RCCGGY 2 cut(s) 33, 79
Cfr13I GGNCC 1 cut(s) 232
CseI GACGC 3 cut(s) 39, 120, 269
Csp6I GTAC 1 cut(s) 356
CviAII CATG 1 cut(s) 259
CviJI RGCY 8 cut(s) 5, 52, 166, 194, 390, 439, 485, 536
CviKI_1 RGCY 8 cut(s) 5, 52, 166, 194, 390, 439, 485, 536
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 2 cut(s) 195, 386
DpnI GATC 4 cut(s) 384, 477, 520, 549
DpnII GATC 4 cut(s) 382, 475, 518, 547
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 439
Eco147I AGGCCT 1 cut(s) 52
Eco24I GRGCYC 2 cut(s) 196, 441
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 439
Eco57I CTGAAG 1 cut(s) 516
EcoICRI GAGCTC 1 cut(s) 439
EcoO109I RGGNCCY 1 cut(s) 232
EcoRII CCWGG 2 cut(s) 23, 183
EcoRV GATATC 1 cut(s) 409
EcoT38I GRGCYC 2 cut(s) 196, 441
FaeI CATG 1 cut(s) 262
FaiI YATR 8 cut(s) 239, 260, 270, 360, 450, 452, 580, 582
FatI CATG 1 cut(s) 258
FauNDI CATATG 1 cut(s) 450
FbaI TGATCA 1 cut(s) 475
Fnu4HI GCNGC 3 cut(s) 486, 537, 540
FokI GGATG 2 cut(s) 167, 239
FriOI GRGCYC 2 cut(s) 196, 441
Fsp4HI GCNGC 3 cut(s) 486, 537, 540
FspBI CTAG 2 cut(s) 48, 54
FspI TGCGCA 1 cut(s) 335
GlaI GCGC 4 cut(s) 218, 248, 250, 335
GluI GCNGC 3 cut(s) 486, 537, 540
GsuI CTGGAG 1 cut(s) 206
HaeIII GGCC 2 cut(s) 5, 52
HapII CCGG 4 cut(s) 34, 80, 134, 418
HgaI GACGC 3 cut(s) 39, 120, 269
HhaI GCGC 4 cut(s) 219, 249, 251, 336
Hin1I GRCGYC 3 cut(s) 31, 112, 141
Hin1II CATG 1 cut(s) 262
Hin6I GCGC 4 cut(s) 217, 247, 249, 334
HinP1I GCGC 4 cut(s) 217, 247, 249, 334
HincII GTYRAC 1 cut(s) 284
HindII GTYRAC 1 cut(s) 284
HinfI GANTC 6 cut(s) 8, 92, 98, 154, 222, 563
HpaII CCGG 4 cut(s) 34, 80, 134, 418
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 284
Hpy188I TCNGA 3 cut(s) 91, 97, 198
Hpy188III TCNNGA 3 cut(s) 134, 418, 479
Hpy8I GTNNAC 1 cut(s) 284
Hpy99I CGWCG 2 cut(s) 33, 143
HpyAV CCTTC 3 cut(s) 140, 193, 491
HpyCH4III ACNGT 1 cut(s) 425
HpyCH4IV ACGT 3 cut(s) 42, 141, 212
HpyCH4V TGCA 2 cut(s) 542, 575
HpyF10VI GCNNNNNNNGC 2 cut(s) 34, 445
HpyF3I CTNAG 2 cut(s) 195, 386
HpySE526I ACGT 3 cut(s) 42, 141, 212
Hsp92I GRCGYC 3 cut(s) 31, 112, 141
Hsp92II CATG 1 cut(s) 262
HspAI GCGC 4 cut(s) 217, 247, 249, 334
Kpn2I TCCGGA 2 cut(s) 133, 417
KroI GCCGGC 1 cut(s) 33
KroNI GCCGGC 1 cut(s) 35
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 4 cut(s) 382, 475, 518, 547
LmnI GCTCC 2 cut(s) 444, 500
Lsp1109I GCAGC 3 cut(s) 472, 523, 526
LweI GCATC 2 cut(s) 337, 584
MaeI CTAG 2 cut(s) 48, 54
MaeII ACGT 3 cut(s) 42, 141, 212
MaeIII GTNAC 2 cut(s) 43, 142
MalI GATC 4 cut(s) 384, 477, 520, 549
MboI GATC 4 cut(s) 382, 475, 518, 547
MboII GAAGA 1 cut(s) 547
MflI RGATCY 2 cut(s) 518, 547
MhlI GDGCHC 2 cut(s) 196, 441
MluCI AATT 4 cut(s) 291, 301, 338, 471
MlyI GAGTC 3 cut(s) 17, 101, 557
MnlI CCTC 3 cut(s) 22, 101, 183
MreI CGCCGGCG 1 cut(s) 33
MroI TCCGGA 2 cut(s) 133, 417
MroNI GCCGGC 1 cut(s) 33
MseI TTAA 2 cut(s) 294, 491
MslI CAYNNNNRTG 1 cut(s) 257
MspI CCGG 4 cut(s) 34, 80, 134, 418
MspR9I CCNGG 2 cut(s) 25, 185
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 2 cut(s) 25, 185
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 2 cut(s) 34, 445
NaeI GCCGGC 1 cut(s) 35
NdeI CATATG 1 cut(s) 450
NdeII GATC 4 cut(s) 382, 475, 518, 547
NgoMIV GCCGGC 1 cut(s) 33
NlaIII CATG 1 cut(s) 262
NlaIV GGNNCC 1 cut(s) 234
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 2 cut(s) 43, 142
NsbI TGCGCA 1 cut(s) 335
PalAI GGCGCGCC 1 cut(s) 247
PauI GCGCGC 1 cut(s) 247
PceI AGGCCT 1 cut(s) 52
PctI GAATGC 1 cut(s) 503
PdiI GCCGGC 1 cut(s) 35
PfeI GAWTC 3 cut(s) 98, 154, 222
PflFI GACNNNGTC 1 cut(s) 140
PfoI TCCNGGA 1 cut(s) 183
PkrI GCNGC 3 cut(s) 487, 538, 541
PleI GAGTC 3 cut(s) 16, 100, 557
PpsI GAGTC 3 cut(s) 16, 100, 557
PpuMI RGGWCCY 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 441
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 2 cut(s) 23, 183
PspGI CCWGG 2 cut(s) 23, 183
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 1 cut(s) 232
PspPPI RGGWCCY 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 225
PsuI RGATCY 2 cut(s) 518, 547
PsyI GACNNNGTC 1 cut(s) 140
PteI GCGCGC 1 cut(s) 247
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
RseI CAYNNNNRTG 1 cut(s) 257
SacI GAGCTC 1 cut(s) 441
SaqAI TTAA 2 cut(s) 294, 491
SatI GCNGC 3 cut(s) 486, 537, 540
Sau3AI GATC 4 cut(s) 382, 475, 518, 547
Sau96I GGNCC 1 cut(s) 232
SchI GAGTC 3 cut(s) 17, 101, 557
ScrFI CCNGG 2 cut(s) 25, 185
SduI GDGCHC 2 cut(s) 196, 441
SfaNI GCATC 2 cut(s) 337, 584
SgrAI CRCCGGYG 1 cut(s) 33
SgsI GGCGCGCC 1 cut(s) 247
SinI GGWCC 1 cut(s) 232
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 4 cut(s) 291, 301, 338, 471
SseBI AGGCCT 1 cut(s) 52
SsiI CCGC 2 cut(s) 37, 147
SspMI CTAG 2 cut(s) 48, 54
SstI GAGCTC 1 cut(s) 441
StuI AGGCCT 1 cut(s) 52
StyD4I CCNGG 2 cut(s) 23, 183
TaaI ACNGT 1 cut(s) 425
TaiI ACGT 3 cut(s) 45, 144, 215
TaqI TCGA 2 cut(s) 152, 177
TasI AATT 4 cut(s) 291, 301, 338, 471
TatI WGTACW 1 cut(s) 355
TfiI GAWTC 3 cut(s) 98, 154, 222
Tru1I TTAA 2 cut(s) 294, 491
Tru9I TTAA 2 cut(s) 294, 491
TscAI CASTG 1 cut(s) 108
TseFI GTSAC 2 cut(s) 43, 142
TseI GCWGC 3 cut(s) 485, 536, 539
Tsp45I GTSAC 2 cut(s) 43, 142
TspDTI ATGAA 1 cut(s) 263
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 1 cut(s) 108
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 2 cut(s) 301, 338
XspI CTAG 2 cut(s) 48, 54
ZraI GACGTC 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.