Rh5DG116900

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0016316
Physical Location & Seq
Forward (+)
10579055 .. 10580616
1562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG116900.1

Sequence Viewer

Length: 1056 bp
ATGGCCGAGTCCTCAAACAAAACCTGGCGACACCGGCGGAACGTGACTAGGCCCAGAAAAGCAAAGAAACAAACCCTACCGGCGTTATCAGAGTCAGAATCACTGCGAGGACGCCCGAACTGGATTGACCTTCCGGACGACGTCACCGCTTCGATTCTGTCACGGCTGTCAATGTTCGACATCCTGGAGAGGGCTCAGAAGGTGTGCCTGACGTGGCGCAGAATCTGTAAGGACCCTATGACTTGGCGCGCCATCCACATGGAGTTCCATATTCATTTGCGCCAACAAAAAATTGAAGGAAATTTCACTTCCGGCAAAAGAAACAAGCATCTGCGCAAATTCCACAACTTCAATGTACATAAGATGTGCCACCACGCCATTGATCTTAGCTGTGGCAATCTCGTAGATATCAGTATCCGGAACTGTGGCACAGATGAGCTCCTTGAGCATATGACTGATAGTTCGAGGGGAATCAGACGCCTCAGTATTGTGTTTTCAGCTTACATAACAATGGAGGGACTACGTAAAGTGGCTTCGAAGCTTCCGCTGTTGGAGGATCTTGAAATCTCATACACCCCACGGATTAGTGAAATATTCCGAGGCTTTCACAGCTGTGAATATCTTGAAGTGATTGGCCGTTCTTGCCCTCTTTTGAAATCTTTGAAATTGAACATGCACCCCGGGGGTATGTTCAACAGCATAGACAATGACGATGCATTTGCGATAGCCAGAACAATGCATGGGTTAAAGAACCTCCAGCTTTTTGGAAATTGGATGAATCATCAGGGATTGCACGCCATTCTTGTAGGTTGTCCGCATCTTGAGTCACTTGATCTCCGTCAATGTATACATCTTTATCTGAATGGTAAGGGATGGGGATGGCGCGCCATTCGTGAAGGTTGTTCATCACATCTTCAAGATTTGACAAGAAGATGTTTTGAACAAATTACAAAACTGTGGCTTCCTGACGATTCTGTACATGACTATGAGCATCTTGCCATTCATAAAATTGAAAACAGACAATACGAAGAGTATAATAAAGATATAATGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

351

Amino Acids

40.96

Weight (kDa)

9.09

Isoelectric Point (pI)

53.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 41 - 87 8e-09 F-box-like
F-box PF00646 41 - 83 6.8e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016316)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g26250 FvH4_5g19531
rosa_chinensis RchiOBHm_Chr5g0015241
rosa_laevigata RLG00000032180
rosa_multiflora Rmu_sc0002209.1_g000024
rosa_roxburghii Rroxscaffold_1G00061330
rosa_rugosa Rorug05G0024900
rosa_samantha Rh5AG119400 Rh5AG119500 Rh5BG117500 Rh5BG118300 Rh5CG128600 Rh5DG116600 Rh5DG116900
rosa_wichuraiana Rw5G010320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 144
Acc16I TGCGCA 1 cut(s) 335
AccI GTMKAC 1 cut(s) 847
AccII CGCG 2 cut(s) 249, 885
AccIII TCCGGA 2 cut(s) 133, 417
AciI CCGC 4 cut(s) 37, 147, 545, 815
AclWI GGATC 1 cut(s) 564
AcoI YGGCCR 2 cut(s) 3, 634
AcsI RAATTY 2 cut(s) 301, 338
AcyI GRCGYC 3 cut(s) 112, 141, 478
AfaI GTAC 2 cut(s) 357, 978
AfiI CCNNNNNNNGG 1 cut(s) 190
AjiI CACGTC 1 cut(s) 213
AjnI CCWGG 2 cut(s) 23, 183
AleI CACNNNNGTG 1 cut(s) 612
AluBI AGCT 6 cut(s) 390, 439, 500, 541, 612, 760
AluI AGCT 6 cut(s) 390, 439, 500, 541, 612, 760
Alw21I GWGCWC 1 cut(s) 441
AlwI GGATC 1 cut(s) 564
AlwNI CAGNNNCTG 1 cut(s) 225
Ama87I CYCGRG 1 cut(s) 680
Aor13HI TCCGGA 2 cut(s) 133, 417
AoxI GGCC 3 cut(s) 3, 50, 634
ApoI RAATTY 2 cut(s) 301, 338
ArsI GACNNNNNNTTYG 4 cut(s) 445, 477, 701, 733
AscI GGCGCGCC 2 cut(s) 247, 883
AspLEI GCGC 7 cut(s) 219, 249, 251, 282, 336, 885, 887
AspS9I GGNCC 2 cut(s) 51, 232
AsuC2I CCSGG 2 cut(s) 681, 682
AsuHPI GGTGA 1 cut(s) 136
AsuII TTCGAA 1 cut(s) 536
AvaI CYCGRG 1 cut(s) 680
AvaII GGWCC 1 cut(s) 232
BanII GRGCYC 2 cut(s) 196, 441
BarI GAAGNNNNNNTAC 2 cut(s) 517, 549
Bbv12I GWGCWC 1 cut(s) 441
BccI CCATC 3 cut(s) 260, 867, 873
BceAI ACGGC 2 cut(s) 179, 621
BcgI CGANNNNNNTGC 2 cut(s) 701, 735
BciT130I CCWGG 2 cut(s) 25, 185
BciVI GTATCC 1 cut(s) 425
BcnI CCSGG 2 cut(s) 681, 682
BfaI CTAG 2 cut(s) 48, 1054
BfuI GTATCC 1 cut(s) 425
Bme1390I CCNGG 4 cut(s) 25, 185, 681, 682
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 680
BmgBI CACGTC 1 cut(s) 213
BmgT120I GGNCC 2 cut(s) 51, 232
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 4 cut(s) 25, 185, 681, 682
BmsI GCATC 4 cut(s) 337, 703, 826, 1000
BpmI CTGGAG 2 cut(s) 206, 740
Bpu14I TTCGAA 1 cut(s) 536
BpuEI CTTGAG 2 cut(s) 464, 842
BpuMI CCSGG 2 cut(s) 681, 682
BsaAI YACGTR 1 cut(s) 524
BsaHI GRCGYC 3 cut(s) 112, 141, 478
BsaJI CCNNGG 5 cut(s) 578, 598, 679, 680, 681
BsaWI WCCGGW 2 cut(s) 133, 417
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse118I RCCGGY 2 cut(s) 33, 79
Bse1I ACTGG 1 cut(s) 125
BseAI TCCGGA 2 cut(s) 133, 417
BseBI CCWGG 2 cut(s) 25, 185
BseDI CCNNGG 5 cut(s) 578, 598, 679, 680, 681
BseGI GGATG 5 cut(s) 180, 252, 780, 878, 884
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 2 cut(s) 209, 496
BseNI ACTGG 1 cut(s) 125
BsePI GCGCGC 2 cut(s) 247, 883
Bsh1236I CGCG 2 cut(s) 249, 885
BshFI GGCC 3 cut(s) 5, 52, 636
BsiHKAI GWGCWC 1 cut(s) 441
BsiHKCI CYCGRG 1 cut(s) 680
BsiSI CCGG 6 cut(s) 34, 80, 134, 312, 418, 681
BslFI GGGAC 1 cut(s) 531
BslI CCNNNNNNNGG 1 cut(s) 190
BsmFI GGGAC 1 cut(s) 531
BsnI GGCC 3 cut(s) 5, 52, 636
BsoBI CYCGRG 1 cut(s) 680
Bsp119I TTCGAA 1 cut(s) 536
Bsp1286I GDGCHC 2 cut(s) 196, 441
Bsp13I TCCGGA 2 cut(s) 133, 417
Bsp1407I TGTACA 2 cut(s) 355, 976
Bsp143I GATC 4 cut(s) 382, 556, 832, 1050
BspACI CCGC 4 cut(s) 37, 147, 545, 815
BspANI GGCC 3 cut(s) 5, 52, 636
BspCNI CTCAG 2 cut(s) 208, 495
BspEI TCCGGA 2 cut(s) 133, 417
BspFNI CGCG 2 cut(s) 249, 885
BspLI GGNNCC 1 cut(s) 234
BspPI GGATC 1 cut(s) 564
BspT104I TTCGAA 1 cut(s) 536
BsrFI RCCGGY 2 cut(s) 33, 79
BsrGI TGTACA 2 cut(s) 355, 976
BsrI ACTGG 1 cut(s) 125
BssAI RCCGGY 2 cut(s) 33, 79
BssECI CCNNGG 5 cut(s) 578, 598, 679, 680, 681
BssHII GCGCGC 2 cut(s) 247, 883
BssMI GATC 4 cut(s) 382, 556, 832, 1050
BssNAI GTATAC 1 cut(s) 848
BssNI GRCGYC 3 cut(s) 112, 141, 478
Bst1107I GTATAC 1 cut(s) 848
Bst2UI CCWGG 2 cut(s) 25, 185
Bst4CI ACNGT 2 cut(s) 425, 957
Bst6I CTCTTC 1 cut(s) 1023
BstACI GRCGYC 3 cut(s) 112, 141, 478
BstAUI TGTACA 2 cut(s) 355, 976
BstBAI YACGTR 1 cut(s) 524
BstBI TTCGAA 1 cut(s) 536
BstC8I GCNNGC 3 cut(s) 249, 795, 885
BstDEI CTNAG 3 cut(s) 195, 386, 482
BstDSI CCRYGG 1 cut(s) 578
BstF5I GGATG 5 cut(s) 180, 252, 780, 878, 884
BstFNI CGCG 2 cut(s) 249, 885
BstHHI GCGC 7 cut(s) 219, 249, 251, 282, 336, 885, 887
BstKTI GATC 4 cut(s) 385, 559, 835, 1053
BstMBI GATC 4 cut(s) 382, 556, 832, 1050
BstMWI GCNNNNNNNGC 4 cut(s) 34, 445, 609, 642
BstNI CCWGG 2 cut(s) 25, 185
BstNSI RCATGY 1 cut(s) 676
BstSCI CCNGG 4 cut(s) 23, 183, 679, 680
BstSNI TACGTA 1 cut(s) 524
BstUI CGCG 2 cut(s) 249, 885
BstX2I RGATCY 1 cut(s) 556
BstXI CCANNNNNNTGG 2 cut(s) 259, 764
BstYI RGATCY 1 cut(s) 556
BstZ17I GTATAC 1 cut(s) 848
BsuI GTATCC 1 cut(s) 425
BsuRI GGCC 3 cut(s) 5, 52, 636
BtgI CCRYGG 1 cut(s) 578
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 5 cut(s) 180, 252, 780, 878, 884
BtsI GCAGTG 1 cut(s) 101
BtsIMutI CAGTG 1 cut(s) 101
Cac8I GCNNGC 3 cut(s) 249, 795, 885
CaiI CAGNNNCTG 1 cut(s) 225
CfoI GCGC 7 cut(s) 219, 249, 251, 282, 336, 885, 887
Cfr10I RCCGGY 2 cut(s) 33, 79
Cfr13I GGNCC 2 cut(s) 51, 232
Cfr9I CCCGGG 1 cut(s) 680
CseI GACGC 2 cut(s) 120, 486
Csp6I GTAC 2 cut(s) 356, 977
CviAII CATG 4 cut(s) 259, 673, 740, 980
CviQI GTAC 2 cut(s) 356, 977
DdeI CTNAG 3 cut(s) 195, 386, 482
DpnI GATC 4 cut(s) 384, 558, 834, 1052
DpnII GATC 4 cut(s) 382, 556, 832, 1050
EaeI YGGCCR 2 cut(s) 3, 634
Eam1104I CTCTTC 1 cut(s) 1023
EarI CTCTTC 1 cut(s) 1023
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 439
Eco105I TACGTA 1 cut(s) 524
Eco24I GRGCYC 2 cut(s) 196, 441
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 439
Eco88I CYCGRG 1 cut(s) 680
EcoICRI GAGCTC 1 cut(s) 439
EcoO109I RGGNCCY 1 cut(s) 232
EcoRII CCWGG 2 cut(s) 23, 183
EcoRV GATATC 1 cut(s) 409
EcoT22I ATGCAT 2 cut(s) 718, 741
EcoT38I GRGCYC 2 cut(s) 196, 441
FaeI CATG 4 cut(s) 262, 676, 743, 983
FaqI GGGAC 1 cut(s) 531
FatI CATG 4 cut(s) 258, 672, 739, 979
FauNDI CATATG 1 cut(s) 450
FblI GTMKAC 1 cut(s) 847
FokI GGATG 5 cut(s) 167, 239, 787, 885, 891
FriOI GRGCYC 2 cut(s) 196, 441
FspBI CTAG 2 cut(s) 48, 1054
FspI TGCGCA 1 cut(s) 335
GlaI GCGC 7 cut(s) 218, 248, 250, 281, 335, 884, 886
GsuI CTGGAG 2 cut(s) 206, 740
HaeIII GGCC 3 cut(s) 5, 52, 636
HapII CCGG 6 cut(s) 34, 80, 134, 312, 418, 681
HgaI GACGC 2 cut(s) 120, 486
HhaI GCGC 7 cut(s) 219, 249, 251, 282, 336, 885, 887
Hin1I GRCGYC 3 cut(s) 112, 141, 478
Hin1II CATG 4 cut(s) 262, 676, 743, 983
Hin6I GCGC 7 cut(s) 217, 247, 249, 280, 334, 883, 885
HinP1I GCGC 7 cut(s) 217, 247, 249, 280, 334, 883, 885
HindIII AAGCTT 1 cut(s) 539
HinfI GANTC 9 cut(s) 8, 92, 98, 154, 222, 471, 778, 824, 971
HpaII CCGG 6 cut(s) 34, 80, 134, 312, 418, 681
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 848
Hpy188I TCNGA 6 cut(s) 91, 97, 198, 476, 599, 861
Hpy188III TCNNGA 8 cut(s) 134, 418, 560, 623, 821, 893, 917, 965
Hpy8I GTNNAC 1 cut(s) 848
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 4 cut(s) 140, 193, 290, 890
HpyCH4III ACNGT 2 cut(s) 425, 957
HpyCH4IV ACGT 4 cut(s) 42, 141, 212, 523
HpyCH4V TGCA 4 cut(s) 676, 716, 739, 793
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 445, 609, 642
HpyF3I CTNAG 3 cut(s) 195, 386, 482
HpySE526I ACGT 4 cut(s) 42, 141, 212, 523
Hsp92I GRCGYC 3 cut(s) 112, 141, 478
Hsp92II CATG 4 cut(s) 262, 676, 743, 983
HspAI GCGC 7 cut(s) 217, 247, 249, 280, 334, 883, 885
Kpn2I TCCGGA 2 cut(s) 133, 417
Kzo9I GATC 4 cut(s) 382, 556, 832, 1050
LmnI GCTCC 1 cut(s) 444
LweI GCATC 4 cut(s) 337, 703, 826, 1000
MaeI CTAG 2 cut(s) 48, 1054
MaeII ACGT 4 cut(s) 42, 141, 212, 523
MaeIII GTNAC 4 cut(s) 43, 142, 159, 825
MalI GATC 4 cut(s) 384, 558, 834, 1052
MboI GATC 4 cut(s) 382, 556, 832, 1050
MboII GAAGA 3 cut(s) 905, 942, 1040
MflI RGATCY 1 cut(s) 556
MhlI GDGCHC 2 cut(s) 196, 441
MluCI AATT 7 cut(s) 291, 301, 338, 665, 769, 945, 1008
MlyI GAGTC 3 cut(s) 17, 101, 833
MmeI TCCRAC 1 cut(s) 531
Mph1103I ATGCAT 2 cut(s) 718, 741
MroI TCCGGA 2 cut(s) 133, 417
MseI TTAA 1 cut(s) 746
MslI CAYNNNNRTG 4 cut(s) 257, 509, 612, 984
MspA1I CMGCKG 2 cut(s) 547, 612
MspI CCGG 6 cut(s) 34, 80, 134, 312, 418, 681
MspR9I CCNGG 4 cut(s) 25, 185, 681, 682
MvaI CCWGG 2 cut(s) 25, 185
MvnI CGCG 2 cut(s) 249, 885
MwoI GCNNNNNNNGC 4 cut(s) 34, 445, 609, 642
NciI CCSGG 2 cut(s) 681, 682
NdeI CATATG 1 cut(s) 450
NdeII GATC 4 cut(s) 382, 556, 832, 1050
NlaIII CATG 4 cut(s) 262, 676, 743, 983
NlaIV GGNNCC 1 cut(s) 234
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 4 cut(s) 43, 142, 159, 825
NsbI TGCGCA 1 cut(s) 335
NsiI ATGCAT 2 cut(s) 718, 741
NspI RCATGY 1 cut(s) 676
NspV TTCGAA 1 cut(s) 536
OliI CACNNNNGTG 1 cut(s) 612
PalAI GGCGCGCC 2 cut(s) 247, 883
PauI GCGCGC 2 cut(s) 247, 883
PfeI GAWTC 6 cut(s) 98, 154, 222, 471, 778, 971
PflFI GACNNNGTC 1 cut(s) 140
PfoI TCCNGGA 1 cut(s) 183
PleI GAGTC 3 cut(s) 16, 100, 832
PpsI GAGTC 3 cut(s) 16, 100, 832
Ppu21I YACGTR 1 cut(s) 524
PpuMI RGGWCCY 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 441
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 2 cut(s) 23, 183
PspGI CCWGG 2 cut(s) 23, 183
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 2 cut(s) 51, 232
PspPPI RGGWCCY 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 225
PsuI RGATCY 1 cut(s) 556
PsyI GACNNNGTC 1 cut(s) 140
PteI GCGCGC 2 cut(s) 247, 883
PvuII CAGCTG 1 cut(s) 612
RsaI GTAC 2 cut(s) 357, 978
RsaNI GTAC 2 cut(s) 356, 977
RseI CAYNNNNRTG 4 cut(s) 257, 509, 612, 984
SacI GAGCTC 1 cut(s) 441
SaqAI TTAA 1 cut(s) 746
Sau3AI GATC 4 cut(s) 382, 556, 832, 1050
Sau96I GGNCC 2 cut(s) 51, 232
SchI GAGTC 3 cut(s) 17, 101, 833
ScrFI CCNGG 4 cut(s) 25, 185, 681, 682
SduI GDGCHC 2 cut(s) 196, 441
SfaNI GCATC 4 cut(s) 337, 703, 826, 1000
SfuI TTCGAA 1 cut(s) 536
SgrAI CRCCGGYG 1 cut(s) 33
SgsI GGCGCGCC 2 cut(s) 247, 883
SinI GGWCC 1 cut(s) 232
SmaI CCCGGG 1 cut(s) 682
SmiMI CAYNNNNRTG 4 cut(s) 257, 509, 612, 984
SmlI CTYRAG 2 cut(s) 443, 821
SmoI CTYRAG 2 cut(s) 443, 821
SnaBI TACGTA 1 cut(s) 524
Sse9I AATT 7 cut(s) 291, 301, 338, 665, 769, 945, 1008
SsiI CCGC 4 cut(s) 37, 147, 545, 815
SspI AATATT 1 cut(s) 594
SspMI CTAG 2 cut(s) 48, 1054
SstI GAGCTC 1 cut(s) 441
StyD4I CCNGG 4 cut(s) 23, 183, 679, 680
TaaI ACNGT 2 cut(s) 425, 957
TaiI ACGT 4 cut(s) 45, 144, 215, 526
TaqI TCGA 4 cut(s) 152, 177, 464, 536
TasI AATT 7 cut(s) 291, 301, 338, 665, 769, 945, 1008
TatI WGTACW 2 cut(s) 355, 976
TfiI GAWTC 6 cut(s) 98, 154, 222, 471, 778, 971
Tru1I TTAA 1 cut(s) 746
Tru9I TTAA 1 cut(s) 746
TscAI CASTG 1 cut(s) 108
TseFI GTSAC 4 cut(s) 43, 142, 159, 825
Tsp45I GTSAC 4 cut(s) 43, 142, 159, 825
TspDTI ATGAA 4 cut(s) 263, 791, 894, 992
TspGWI ACGGA 2 cut(s) 595, 827
TspMI CCCGGG 1 cut(s) 680
TspRI CASTG 1 cut(s) 108
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 2 cut(s) 301, 338
XceI RCATGY 1 cut(s) 676
XmaI CCCGGG 1 cut(s) 680
XmiI GTMKAC 1 cut(s) 847
XspI CTAG 2 cut(s) 48, 1054
ZraI GACGTC 1 cut(s) 142
Zsp2I ATGCAT 2 cut(s) 718, 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.