Rh5BG118300

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0016316
Physical Location & Seq
Forward (+)
11143468 .. 11143935
468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG118300.1

Sequence Viewer

Length: 468 bp
ATGGAGCATGTTGAAATCTCATACACCCCACGGATTAGTGAAATTTTCGGAGTCTTTAACAGCTGTGAATATCTTGAAGTGATTGGCCGTTCTTGCCCTCTTTTGAAATCTTTGAAATTGAACAAGCACCCCGGGGATATGTTCGACAGCAATGACAATGATGATGCATTTGCGATAGCCAGAACAATGCATGGCTTAAAGAACCTCCAGCTTTTTGGAAATTGGATGAATCATCAGGGATTGCACGCCATTCTTGTAGGTTGTCCGCATCTTGAGTCACTTGATCTCCGTCAATGTAGACATCTTCATCTGAATAGTAAGGGATGGGGATGGCGCGCCATTCATGAAGGTTGTTCATCACATCTGGAAGATTTGACAAGAAATTCTGTACATGACTATGAGCATCTTGCCATTGATAAAATTGAAAACAGACAATACGAAGAGTATAATAAAGATATGATGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

155

Amino Acids

18.0

Weight (kDa)

5.9

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 56 - 126 2.2e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016316)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g26250 FvH4_5g19531
rosa_chinensis RchiOBHm_Chr5g0015241
rosa_laevigata RLG00000032180
rosa_multiflora Rmu_sc0002209.1_g000024
rosa_roxburghii Rroxscaffold_1G00061330
rosa_rugosa Rorug05G0024900
rosa_samantha Rh5AG119400 Rh5AG119500 Rh5BG117500 Rh5BG118300 Rh5CG128600 Rh5DG116600 Rh5DG116900
rosa_wichuraiana Rw5G010320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 298
AccII CGCG 1 cut(s) 336
AciI CCGC 1 cut(s) 266
AcoI YGGCCR 1 cut(s) 85
AcsI RAATTY 2 cut(s) 42, 382
AfaI GTAC 1 cut(s) 390
AgsI TTSAA 6 cut(s) 14, 77, 106, 115, 121, 425
AluBI AGCT 2 cut(s) 63, 211
AluI AGCT 2 cut(s) 63, 211
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 1 cut(s) 85
ApoI RAATTY 2 cut(s) 42, 382
AscI GGCGCGCC 1 cut(s) 334
AspLEI GCGC 2 cut(s) 336, 338
AsuC2I CCSGG 2 cut(s) 132, 133
AvaI CYCGRG 1 cut(s) 131
BccI CCATC 2 cut(s) 318, 324
BceAI ACGGC 1 cut(s) 72
BcnI CCSGG 2 cut(s) 132, 133
BfaI CTAG 1 cut(s) 466
Bme1390I CCNGG 2 cut(s) 132, 133
BmeT110I CYCGRG 1 cut(s) 131
BmrFI CCNGG 2 cut(s) 132, 133
BmsI GCATC 3 cut(s) 154, 277, 412
BpmI CTGGAG 1 cut(s) 191
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 2 cut(s) 132, 133
BsaJI CCNNGG 4 cut(s) 29, 130, 131, 132
BsaXI ACNNNNNCTCC 2 cut(s) 270, 300
Bse3DI GCAATG 1 cut(s) 157
BseDI CCNNGG 4 cut(s) 29, 130, 131, 132
BseGI GGATG 3 cut(s) 231, 329, 335
BseMI GCAATG 1 cut(s) 157
BsePI GCGCGC 1 cut(s) 334
Bsh1236I CGCG 1 cut(s) 336
BshFI GGCC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 1 cut(s) 132
BsnI GGCC 1 cut(s) 87
BsoBI CYCGRG 1 cut(s) 131
Bsp1407I TGTACA 1 cut(s) 388
Bsp143I GATC 2 cut(s) 283, 462
BspACI CCGC 1 cut(s) 266
BspANI GGCC 1 cut(s) 87
BspFNI CGCG 1 cut(s) 336
BspHI TCATGA 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 157
BsrGI TGTACA 1 cut(s) 388
BssECI CCNNGG 4 cut(s) 29, 130, 131, 132
BssHII GCGCGC 1 cut(s) 334
BssMI GATC 2 cut(s) 283, 462
Bst6I CTCTTC 1 cut(s) 435
BstAUI TGTACA 1 cut(s) 388
BstC8I GCNNGC 2 cut(s) 246, 336
BstDSI CCRYGG 1 cut(s) 29
BstF5I GGATG 3 cut(s) 231, 329, 335
BstFNI CGCG 1 cut(s) 336
BstHHI GCGC 2 cut(s) 336, 338
BstKTI GATC 2 cut(s) 286, 465
BstMBI GATC 2 cut(s) 283, 462
BstMWI GCNNNNNNNGC 1 cut(s) 93
BstNSI RCATGY 1 cut(s) 11
BstSCI CCNGG 2 cut(s) 130, 131
BstUI CGCG 1 cut(s) 336
BstXI CCANNNNNNTGG 1 cut(s) 215
BsuRI GGCC 1 cut(s) 87
BtgI CCRYGG 1 cut(s) 29
BtsCI GGATG 3 cut(s) 231, 329, 335
Cac8I GCNNGC 2 cut(s) 246, 336
CciI TCATGA 1 cut(s) 343
CfoI GCGC 2 cut(s) 336, 338
Cfr9I CCCGGG 1 cut(s) 131
Csp6I GTAC 1 cut(s) 389
CviAII CATG 4 cut(s) 8, 191, 344, 392
CviJI RGCY 5 cut(s) 63, 87, 179, 195, 211
CviKI_1 RGCY 5 cut(s) 63, 87, 179, 195, 211
CviQI GTAC 1 cut(s) 389
DpnI GATC 2 cut(s) 285, 464
DpnII GATC 2 cut(s) 283, 462
EaeI YGGCCR 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
Eco88I CYCGRG 1 cut(s) 131
EcoT22I ATGCAT 2 cut(s) 169, 192
FaeI CATG 4 cut(s) 11, 194, 347, 395
FaiI YATR 9 cut(s) 9, 22, 140, 192, 345, 393, 399, 447, 458
FatI CATG 4 cut(s) 7, 190, 343, 391
FblI GTMKAC 1 cut(s) 298
FokI GGATG 3 cut(s) 238, 336, 342
FspBI CTAG 1 cut(s) 466
GlaI GCGC 2 cut(s) 335, 337
GsuI CTGGAG 1 cut(s) 191
HaeIII GGCC 1 cut(s) 87
HapII CCGG 1 cut(s) 132
HhaI GCGC 2 cut(s) 336, 338
Hin1II CATG 4 cut(s) 11, 194, 347, 395
Hin6I GCGC 2 cut(s) 334, 336
HinP1I GCGC 2 cut(s) 334, 336
HinfI GANTC 3 cut(s) 51, 229, 275
HpaII CCGG 1 cut(s) 132
Hpy166II GTNNAC 1 cut(s) 299
Hpy188I TCNGA 2 cut(s) 50, 312
Hpy188III TCNNGA 4 cut(s) 74, 272, 344, 365
Hpy8I GTNNAC 1 cut(s) 299
HpyAV CCTTC 1 cut(s) 341
HpyCH4V TGCA 3 cut(s) 167, 190, 244
HpyF10VI GCNNNNNNNGC 1 cut(s) 93
Hsp92II CATG 4 cut(s) 11, 194, 347, 395
HspAI GCGC 2 cut(s) 334, 336
Kzo9I GATC 2 cut(s) 283, 462
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 5 cut(s) 145, 193, 221, 221, 350
LweI GCATC 3 cut(s) 154, 277, 412
MaeI CTAG 1 cut(s) 466
MaeIII GTNAC 1 cut(s) 276
MalI GATC 2 cut(s) 285, 464
MboI GATC 2 cut(s) 283, 462
MboII GAAGA 3 cut(s) 296, 380, 452
MluCI AATT 5 cut(s) 42, 116, 220, 382, 420
MlyI GAGTC 2 cut(s) 60, 284
MnlI CCTC 2 cut(s) 108, 215
Mph1103I ATGCAT 2 cut(s) 169, 192
MseI TTAA 2 cut(s) 57, 197
MslI CAYNNNNRTG 1 cut(s) 396
MspA1I CMGCKG 1 cut(s) 63
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 2 cut(s) 132, 133
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 1 cut(s) 93
NciI CCSGG 2 cut(s) 132, 133
NdeII GATC 2 cut(s) 283, 462
NlaIII CATG 4 cut(s) 11, 194, 347, 395
NmuCI GTSAC 1 cut(s) 276
NsiI ATGCAT 2 cut(s) 169, 192
NspI RCATGY 1 cut(s) 11
PagI TCATGA 1 cut(s) 343
PalAI GGCGCGCC 1 cut(s) 334
PauI GCGCGC 1 cut(s) 334
PfeI GAWTC 1 cut(s) 229
PleI GAGTC 2 cut(s) 59, 283
PpsI GAGTC 2 cut(s) 59, 283
PteI GCGCGC 1 cut(s) 334
PvuII CAGCTG 1 cut(s) 63
RsaI GTAC 1 cut(s) 390
RsaNI GTAC 1 cut(s) 389
RseI CAYNNNNRTG 1 cut(s) 396
SaqAI TTAA 2 cut(s) 57, 197
Sau3AI GATC 2 cut(s) 283, 462
SchI GAGTC 2 cut(s) 60, 284
ScrFI CCNGG 2 cut(s) 132, 133
SetI ASST 5 cut(s) 65, 207, 213, 262, 352
SfaNI GCATC 3 cut(s) 154, 277, 412
SgsI GGCGCGCC 1 cut(s) 334
SmaI CCCGGG 1 cut(s) 133
SmiMI CAYNNNNRTG 1 cut(s) 396
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 5 cut(s) 42, 116, 220, 382, 420
SsiI CCGC 1 cut(s) 266
SspMI CTAG 1 cut(s) 466
StyD4I CCNGG 2 cut(s) 130, 131
TaqI TCGA 1 cut(s) 144
TasI AATT 5 cut(s) 42, 116, 220, 382, 420
TatI WGTACW 1 cut(s) 388
TfiI GAWTC 1 cut(s) 229
Tru1I TTAA 2 cut(s) 57, 197
Tru9I TTAA 2 cut(s) 57, 197
TseFI GTSAC 1 cut(s) 276
Tsp45I GTSAC 1 cut(s) 276
TspDTI ATGAA 5 cut(s) 242, 296, 332, 345, 360
TspGWI ACGGA 2 cut(s) 46, 278
TspMI CCCGGG 1 cut(s) 131
XapI RAATTY 2 cut(s) 42, 382
XceI RCATGY 1 cut(s) 11
XmaI CCCGGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 298
XspI CTAG 1 cut(s) 466
Zsp2I ATGCAT 2 cut(s) 169, 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.