RLG00000033295

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015860
Physical Location & Seq
Forward (+)
25378695 .. 25382538
3844 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033295

Sequence Viewer

Length: 462 bp
ATGGCAAACAGTAACCTTCCCCGGAGGATCATCAAGGAAACTCAGCGTCTCCTCAGTGAACCTGCTCCGGGAATTAGTGCTTCGCCATCGGAAGACAATATGCGGTATTTCAATGTGATGATCCTTGGCCCGACTCAATCTCCGTATGAAGGAGGAGTTTTCAAGTTGGAACTATTTTTGCCTGAAGAATATCCTATGGCTGCACCAAAGGTTCGGTTTCTCACTAAAATTTATCATCCTAACATTGACAAGCTGGGAAGGATATGCCTTGACATTTTGAAAGACAAGTGGAGTCCTGCTCTACAGATACGAACTGTTCTTCTCAGTATTCAAGCACTTTTGAGTGCTCCAAATCCCGATGATCCGCTCTCTGAGAACATCGCTAAGCACTGGAAATCAAATGAGGCGGAAGCTGTTGAAACAGCCAAGGAATGGACCCGTTTATATGCAAGTGGTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko04624 map04120 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

154

Amino Acids

17.23

Weight (kDa)

6.74

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 9 - 145 6.9e-51 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015860)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78870
fragaria_vesca FvH4_3g18500
malus_domestica MD03G1241600.v1.1 MD11G1260900.v1.1
prunus_persica Prupe.4G167300_v2.0.a1 Prupe.4G167300_v2.0.a1 Prupe.4G167300_v2.0.a1
pyrus_communis pycom03g18840 pycom11g23030
rosa_chinensis RchiOBHm_Chr5g0031261
rosa_laevigata RLG00000033295
rosa_roxburghii Rroxscaffold_1G00049030
rosa_rugosa Rorug05G0123300
rosa_samantha Rh5CG240000
rosa_wichuraiana Rw5G019830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 70
AccB7I CCANNNNNTGG 1 cut(s) 432
AccBSI CCGCTC 1 cut(s) 367
AciI CCGC 3 cut(s) 103, 365, 407
AclWI GGATC 3 cut(s) 35, 115, 356
AcsI RAATTY 1 cut(s) 228
AcuI CTGAAG 1 cut(s) 204
AfiI CCNNNNNNNGG 3 cut(s) 68, 149, 432
AgsI TTSAA 5 cut(s) 112, 163, 280, 332, 419
AluBI AGCT 2 cut(s) 253, 413
AluI AGCT 2 cut(s) 253, 413
Alw21I GWGCWC 1 cut(s) 349
Alw26I GTCTC 1 cut(s) 53
AlwI GGATC 3 cut(s) 35, 115, 356
AoxI GGCC 1 cut(s) 127
ApeKI GCWGC 1 cut(s) 200
ApoI RAATTY 1 cut(s) 228
AspS9I GGNCC 2 cut(s) 128, 435
AsuC2I CCSGG 2 cut(s) 22, 69
AvaII GGWCC 1 cut(s) 435
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 349
BbvI GCAGC 1 cut(s) 187
BccI CCATC 1 cut(s) 94
BcnI CCSGG 2 cut(s) 22, 69
BcoDI GTCTC 1 cut(s) 53
BfmI CTRYAG 1 cut(s) 302
BfuAI ACCTGC 1 cut(s) 70
BisI GCNGC 1 cut(s) 201
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 1 cut(s) 202
Bme1390I CCNGG 2 cut(s) 22, 69
Bme18I GGWCC 1 cut(s) 435
BmgT120I GGNCC 2 cut(s) 128, 435
BmiI GGNNCC 1 cut(s) 437
BmrFI CCNGG 2 cut(s) 22, 69
BpiI GAAGAC 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 283, 315
Bpu1102I GCTNAGC 1 cut(s) 384
BpuMI CCSGG 2 cut(s) 22, 69
BsaJI CCNNGG 3 cut(s) 20, 124, 426
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 149, 432
Bse1I ACTGG 1 cut(s) 395
BseDI CCNNGG 3 cut(s) 20, 124, 426
BseGI GGATG 1 cut(s) 235
BseLI CCNNNNNNNGG 3 cut(s) 68, 149, 432
BseMII CTCAG 4 cut(s) 56, 67, 337, 363
BseNI ACTGG 1 cut(s) 395
BseRI GAGGAG 2 cut(s) 41, 168
BseXI GCAGC 1 cut(s) 187
BseYI CCCAGC 1 cut(s) 253
BsgI GTGCAG 1 cut(s) 186
BshFI GGCC 1 cut(s) 129
BsiHKAI GWGCWC 1 cut(s) 349
BsiSI CCGG 2 cut(s) 22, 68
BslI CCNNNNNNNGG 3 cut(s) 68, 149, 432
BsmAI GTCTC 1 cut(s) 53
BsmBI CGTCTC 1 cut(s) 53
BsnI GGCC 1 cut(s) 129
Bsp1286I GDGCHC 1 cut(s) 349
Bsp143I GATC 3 cut(s) 27, 120, 361
Bsp1720I GCTNAGC 1 cut(s) 384
BspACI CCGC 3 cut(s) 103, 365, 407
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 4 cut(s) 55, 66, 336, 364
BspLI GGNNCC 1 cut(s) 437
BspMI ACCTGC 1 cut(s) 70
BspPI GGATC 3 cut(s) 35, 115, 356
BsrBI CCGCTC 1 cut(s) 367
BsrI ACTGG 1 cut(s) 395
BssECI CCNNGG 3 cut(s) 20, 124, 426
BssMI GATC 3 cut(s) 27, 120, 361
BssT1I CCWWGG 2 cut(s) 124, 426
Bst4CI ACNGT 2 cut(s) 11, 316
BstDEI CTNAG 5 cut(s) 42, 53, 323, 372, 384
BstF5I GGATG 1 cut(s) 235
BstKTI GATC 3 cut(s) 30, 123, 364
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 3 cut(s) 27, 120, 361
BstSCI CCNGG 2 cut(s) 20, 67
BstSFI CTRYAG 1 cut(s) 302
BstV1I GCAGC 1 cut(s) 187
BstV2I GAAGAC 1 cut(s) 99
BsuRI GGCC 1 cut(s) 129
BtgZI GCGATG 1 cut(s) 364
BtsCI GGATG 1 cut(s) 235
BtsIMutI CAGTG 2 cut(s) 61, 388
BveI ACCTGC 1 cut(s) 70
Cfr13I GGNCC 2 cut(s) 128, 435
CseI GACGC 1 cut(s) 35
CviJI RGCY 5 cut(s) 129, 200, 253, 413, 425
CviKI_1 RGCY 5 cut(s) 129, 200, 253, 413, 425
DdeI CTNAG 5 cut(s) 42, 53, 323, 372, 384
DpnI GATC 3 cut(s) 29, 122, 363
DpnII GATC 3 cut(s) 27, 120, 361
EciI GGCGGA 1 cut(s) 422
Eco130I CCWWGG 2 cut(s) 124, 426
Eco47I GGWCC 1 cut(s) 435
Eco57I CTGAAG 1 cut(s) 204
EcoT14I CCWWGG 2 cut(s) 124, 426
ErhI CCWWGG 2 cut(s) 124, 426
Esp3I CGTCTC 1 cut(s) 53
FaiI YATR 6 cut(s) 101, 147, 197, 265, 445, 447
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 1 cut(s) 222
Fsp4HI GCNGC 1 cut(s) 201
GluI GCNGC 1 cut(s) 201
GsaI CCCAGC 1 cut(s) 257
HaeIII GGCC 1 cut(s) 129
HapII CCGG 2 cut(s) 22, 68
HgaI GACGC 1 cut(s) 35
HinfI GANTC 2 cut(s) 133, 292
HpaII CCGG 2 cut(s) 22, 68
Hpy166II GTNNAC 1 cut(s) 59
Hpy188I TCNGA 2 cut(s) 91, 373
Hpy188III TCNNGA 1 cut(s) 356
Hpy8I GTNNAC 1 cut(s) 59
HpyAV CCTTC 3 cut(s) 26, 143, 252
HpyCH4III ACNGT 2 cut(s) 11, 316
HpyCH4V TGCA 2 cut(s) 203, 449
HpyF3I CTNAG 5 cut(s) 42, 53, 323, 372, 384
Kzo9I GATC 3 cut(s) 27, 120, 361
LmnI GCTCC 2 cut(s) 70, 352
LpnPI CCDG 7 cut(s) 35, 75, 81, 195, 239, 309, 376
Lsp1109I GCAGC 1 cut(s) 187
MaeIII GTNAC 1 cut(s) 11
MalI GATC 3 cut(s) 29, 122, 363
MbiI CCGCTC 1 cut(s) 367
MboI GATC 3 cut(s) 27, 120, 361
MboII GAAGA 3 cut(s) 104, 197, 311
MhlI GDGCHC 1 cut(s) 349
MluCI AATT 2 cut(s) 72, 228
MlyI GAGTC 2 cut(s) 127, 301
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 4 cut(s) 18, 62, 146, 397
MspI CCGG 2 cut(s) 22, 68
MspR9I CCNGG 2 cut(s) 22, 69
NciI CCSGG 2 cut(s) 22, 69
NdeII GATC 3 cut(s) 27, 120, 361
NlaIV GGNNCC 1 cut(s) 437
PflMI CCANNNNNTGG 1 cut(s) 432
PfoI TCCNGGA 1 cut(s) 67
PkrI GCNGC 1 cut(s) 202
PleI GAGTC 2 cut(s) 127, 300
PpsI GAGTC 2 cut(s) 127, 300
PspFI CCCAGC 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 437
PspPI GGNCC 2 cut(s) 128, 435
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 3 cut(s) 27, 120, 361
Sau96I GGNCC 2 cut(s) 128, 435
SchI GAGTC 2 cut(s) 127, 301
ScrFI CCNGG 2 cut(s) 22, 69
SduI GDGCHC 1 cut(s) 349
SetI ASST 5 cut(s) 18, 64, 213, 255, 415
SfcI CTRYAG 1 cut(s) 302
SinI GGWCC 1 cut(s) 435
Sse9I AATT 2 cut(s) 72, 228
SsiI CCGC 3 cut(s) 103, 365, 407
StyD4I CCNGG 2 cut(s) 20, 67
StyI CCWWGG 2 cut(s) 124, 426
TaaI ACNGT 2 cut(s) 11, 316
TasI AATT 2 cut(s) 72, 228
TscAI CASTG 2 cut(s) 61, 395
TseI GCWGC 1 cut(s) 200
TspDTI ATGAA 1 cut(s) 162
TspGWI ACGGA 1 cut(s) 132
TspRI CASTG 2 cut(s) 61, 395
Van91I CCANNNNNTGG 1 cut(s) 432
VpaK11BI GGWCC 1 cut(s) 435
XapI RAATTY 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.