Rorug05G0123300

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0015860
Physical Location & Seq
Forward (+)
10890957 .. 10896197
5241 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0123300.1

Sequence Viewer

Length: 2739 bp
ATGGCGGAACTGTCCGAATTGACCGCACTGCGGTTCAAAAACAAAATGGACGACATTACTAACCATCTGGATCGCACGTACCGTGCAACAGATTCGGTGATTTCTGTTAATTGTGACGAAACAGTCGAGCTTGGTCCTTGTTGGCCTAAGCTTCCCCCTGCTTCTGTTCAAGACTTACTCAAGGATGAGCCTTCAAGACTTGTTCCGGTCCACTGTCGTCAAGGGGAAGAAATTTCATGGAATGAGTGGTCGGATAAACCATTCCCAAGTTGGCCAACCCACGATGAAGCCTGGACTACCTGGGTCGCAAAGATGCTTCCTTATTACGGTGATCACTGGAAGAAGGCCAACATCCACGACCCAATAATGCTCTCCGCCAGGCGCTTCTTCCCCGACAAGGCTCTCCTCAGCGCTGCGTTTTGCTTTTGGAACTCATCATCCAACACTATGGACTTGCCCCTCGGGATGATGAGTGTGAGTCTTCTTGACGTTGCTGCCATTGCTGGGTTTAGGCCTCATGGCACCACTTATGACTGCACTGCTCAGTTGAAGCCCAAAACCAAGTTTAGGTCTGGTCGGCACTTCGAATATGGAAGTTGGATAGACTATCATAATAGTGACAAAAGCAACAAAGTTGTCGATCAAGAGCACATAGCGTTCCTCCTCTACTGGCTCTGTAGGTATGTCTTCTGTGTACCTTCAGGGATTAGTAAAGAGTGGTCTCATTTGGCAGAAGTTTTGCATCGAAGAACTGATGTGGCCTTAGGTGAGTTGGTGCTAGCCTCACTGTACAGGGGTTTGCAGAAAATCACTGTCAACCCATTTGATGGTAAAGCTGAGGGGCCACTTTGGCTGTTCCAAATGTGGTTACAGATTTATTTCCCAGACCTAGGCCCGGTTAGTGCATTTCCTGCCGATGAGGTTATGGGGGTTAGGTTGACAGACCAAGTCCCAAAACCACACTCCCCTCGTGATTGCTTTGCAACGTTGTGGAGTTTCAAATCAAACCCATGTTGGACGCACACATTGACGAGAAGGTATCCCGGTTTCCTAAGGAATAGGTTCATAGATGGTCCAACCTCTGATGATGGTGCCTTAGATGAGGAAATACAAGCTGTGCTAACGAGTGTGGTGGTGCAAAGAGATCTGCTGTATGGAGGGATGAAAAATGGTAGACATTATGGAGTCCAGGTCGAATTTTATGGCCCGAACCATTTCAGTAGGCAGTTTGGTTGTATACAAGGAATTCCTTACTCTGGTCATAAAAGCGTTAATTGTTATACCTCATGGAGGGATGTACTAAAAGGTGCTGCGGAAATCAGAGGCGTGTTAAATTCCAGTTGTTCCACAAAGTTGAAACCATTCCTCACCAGGTATGTGCTAACCTCTGAAACTACCCCAGGCTTTGACAAATGGTGGCATGGTCAAACCAAAGATCAATTTTCGGTAGAACATGATGGGCGCTTTGCTATCTTATATATGCATTGTCCATTCCGGGATAGCCATCATCTAGAAGTTCCAGGCTTTCCCCCTGCTGATGGAGAAAGGGAAGAAGGTGAAGAGGGAGAAGCTAAAGTTGAAAGCCCTCTCCCTACCGACAAGAATCGAAAGAGGAAAGGCAACACAAATGCTTTAAAATCAGCTAGGCAGAAGACTACTCAAGTCACAGTATCTGGAATGACGACAAATGGATCCAATAATGAAGAAGCAGAGGAAATTGATGGCCGTCATACCCTGAATGTTGATGGCTTGTCTGGAGCTCAAATTAAGGAATCCACTGTTGACCACCAAAATGCTGTTGTTAAGCTAAAACATATAACTTCAACTTCTAAAAATACCTTCAGATCTATGAAAAAGCTGAGGTCAGATTTTTCAAGTGACACCTCCGAAAACGCTGATAACATGGGAACTGAAGAACCCAAACAGACTGAAGGGCCATTGATGGGAACTGGGGAGTTAGGAAGTAAACAAATGTATAGCGATGCTGCTGCCTTCGAAACTGCCCCCTTACTTACCAGTATGCACCCAGCTCAAAGTGAGCCAACCACGCCAGTTCAAGGATTACTAGACGGCAATGTGAGGACCCTCTCAAGAGAGGAAAAGTTGAAAAAGTTCGAAAACATTGAAAATAGTGTGGCAAACTCATCTGTCGAAGCTTCCAAAGCTGAAGATGATCATGCTTATTCCGTTATTGACGACGTGGTGATTCGAGGCACGAGGGCGATTTTTCATGATGGCCAGGTCCCAGCAGACCTAAGAGAGGCATTAAATTATGTTCGAGGCAGAAGTCGGTTAAGCCATGTTTTGATTGATGCTCTTGATAAGTATTTTAAGGAGCTTAATTGCGCCCAGACGAAAATGACTATGAATTTCAATAAGTTGACGTTGCTCGAAGATGAAGTTCGACGAGATAAGGACATCAGGGATCAGCTGGATAAAGCATCAAATGAAGGAAAGCAGTTGCAAATTGCTTCAATACAAAGAGCGGAGGAAATTTCTCGACTTGATGAAGAAATAAAGGTACTTGAAGCAGAGAGAGAGGAGGAAGAGCGTATGATGGCACTAGATAAAAATATTATTGATGAGAAATTTGCACTCATTAGTTCCTTTGAACTTGAAATGTCCAAGCACCCCGATCGTGGTTCTGAAATGATTGCCTGCCAAATAAAGGATAAACTTTACGATATGAGGAGTGACTCTATACGTCGGGCCATAAACGAGCTACTTTCTAATATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko04624 map04120 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

912

Amino Acids

103.35

Weight (kDa)

5.82

Isoelectric Point (pI)

42.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 118 - 423 5.4e-29 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015860)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78870
fragaria_vesca FvH4_3g18500
malus_domestica MD03G1241600.v1.1 MD11G1260900.v1.1
prunus_persica Prupe.4G167300_v2.0.a1 Prupe.4G167300_v2.0.a1 Prupe.4G167300_v2.0.a1
pyrus_communis pycom03g18840 pycom11g23030
rosa_chinensis RchiOBHm_Chr5g0031261
rosa_laevigata RLG00000033295
rosa_roxburghii Rroxscaffold_1G00049030
rosa_rugosa Rorug05G0123300
rosa_samantha Rh5CG240000
rosa_wichuraiana Rw5G019830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
AccB1I GGYRCC 2 cut(s) 521, 1091
AccB7I CCANNNNNTGG 1 cut(s) 827
AccBSI CCGCTC 1 cut(s) 2486
AccI GTMKAC 2 cut(s) 1174, 1237
AciI CCGC 6 cut(s) 5, 24, 31, 375, 1313, 2486
AclI AACGTT 1 cut(s) 986
AclWI GGATC 4 cut(s) 78, 1686, 1699, 2433
AcoI YGGCCR 3 cut(s) 272, 1723, 2236
AcsI RAATTY 7 cut(s) 231, 1196, 1245, 1333, 2368, 2493, 2588
AcuI CTGAAG 5 cut(s) 684, 1825, 1932, 1950, 2187
AfaI GTAC 5 cut(s) 80, 696, 791, 1299, 2523
AfeI AGCGCT 1 cut(s) 412
AjiI CACGTC 1 cut(s) 2200
AjnI CCWGG 8 cut(s) 290, 299, 377, 1188, 1370, 1399, 1519, 2238
AloI GAACNNNNNNTCC 2 cut(s) 422, 454
Alw21I GWGCWC 2 cut(s) 651, 1762
Alw26I GTCTC 1 cut(s) 726
AlwI GGATC 4 cut(s) 78, 1686, 1699, 2433
AlwNI CAGNNNCTG 1 cut(s) 1673
Ama87I CYCGRG 1 cut(s) 461
Aor51HI AGCGCT 1 cut(s) 412
ApeKI GCWGC 5 cut(s) 413, 494, 1310, 1985, 1988
ApoI RAATTY 7 cut(s) 231, 1196, 1245, 1333, 2368, 2493, 2588
Asp700I GAANNNNTTC 4 cut(s) 1061, 1214, 1361, 2111
AspA2I CCTAGG 1 cut(s) 889
AspLEI GCGC 4 cut(s) 384, 413, 1464, 2348
AsuC2I CCSGG 3 cut(s) 896, 1044, 1496
AsuHPI GGTGA 6 cut(s) 109, 341, 779, 1360, 1568, 2215
AsuII TTCGAA 3 cut(s) 585, 1995, 2115
AsuNHI GCTAGC 1 cut(s) 778
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 5 cut(s) 134, 208, 1073, 2082, 2242
AvrII CCTAGG 1 cut(s) 889
AxyI CCTNAGG 2 cut(s) 763, 1052
BalI TGGCCA 2 cut(s) 274, 2238
BamHI GGATCC 1 cut(s) 1691
BanI GGYRCC 2 cut(s) 521, 1091
BanII GRGCYC 1 cut(s) 1762
BauI CACGAG 2 cut(s) 969, 2215
BbsI GAAGAC 3 cut(s) 473, 679, 1658
Bbv12I GWGCWC 2 cut(s) 651, 1762
BbvCI CCTCAGC 3 cut(s) 407, 837, 1859
BbvI GCAGC 5 cut(s) 400, 481, 1297, 1972, 1975
BceAI ACGGC 2 cut(s) 1710, 2086
BcgI CGANNNNNNTGC 6 cut(s) 75, 109, 1970, 2004, 2618, 2652
BciT130I CCWGG 8 cut(s) 292, 301, 379, 1190, 1372, 1401, 1521, 2240
BciVI GTATCC 1 cut(s) 1050
BclI TGATCA 2 cut(s) 331, 2173
BcnI CCSGG 3 cut(s) 896, 1044, 1496
BcoDI GTCTC 1 cut(s) 726
BfaI CTAG 6 cut(s) 779, 890, 1511, 1644, 2066, 2564
BfmI CTRYAG 1 cut(s) 676
BfoI RGCGCY 3 cut(s) 385, 414, 1465
BfuI GTATCC 1 cut(s) 1050
BglI GCCNNNNNGGC 1 cut(s) 850
BglII AGATCT 2 cut(s) 1144, 1844
BisI GCNGC 5 cut(s) 414, 495, 1311, 1986, 1989
BlnI CCTAGG 1 cut(s) 889
BlsI GCNGC 5 cut(s) 415, 496, 1312, 1987, 1990
Bme18I GGWCC 5 cut(s) 134, 208, 1073, 2082, 2242
BmeT110I CYCGRG 1 cut(s) 461
BmgBI CACGTC 1 cut(s) 2200
BmiI GGNNCC 6 cut(s) 523, 843, 1093, 1693, 2084, 2244
BmrI ACTGGG 1 cut(s) 1959
BmsI GCATC 5 cut(s) 303, 751, 1972, 2302, 2450
BmtI GCTAGC 1 cut(s) 782
BmuI ACTGGG 1 cut(s) 1959
BpiI GAAGAC 3 cut(s) 473, 679, 1658
BpmI CTGGAG 1 cut(s) 1776
Bpu10I CCTNAGC 4 cut(s) 147, 407, 837, 1859
Bpu14I TTCGAA 3 cut(s) 585, 1995, 2115
BpuEI CTTGAG 3 cut(s) 164, 1644, 2074
BpuMI CCSGG 3 cut(s) 896, 1044, 1496
BsaAI YACGTR 1 cut(s) 78
BsaBI GATNNNNATC 1 cut(s) 1503
BsaI GGTCTC 1 cut(s) 726
BsaJI CCNNGG 4 cut(s) 300, 460, 889, 1399
BsaWI WCCGGW 1 cut(s) 205
BsaXI ACNNNNNCTCC 2 cut(s) 1176, 1206
Bse1I ACTGG 6 cut(s) 341, 674, 1338, 1954, 2016, 2051
Bse21I CCTNAGG 2 cut(s) 763, 1052
Bse3DI GCAATG 2 cut(s) 498, 2080
Bse8I GATNNNNATC 1 cut(s) 1503
BseBI CCWGG 8 cut(s) 292, 301, 379, 1190, 1372, 1401, 1521, 2240
BseDI CCNNGG 4 cut(s) 300, 460, 889, 1399
BseGI GGATG 6 cut(s) 190, 351, 437, 471, 1167, 1300
BseJI GATNNNNATC 1 cut(s) 1503
BseMI GCAATG 2 cut(s) 498, 2080
BseMII CTCAG 4 cut(s) 421, 557, 828, 1850
BseNI ACTGG 6 cut(s) 341, 674, 1338, 1954, 2016, 2051
BseRI GAGGAG 4 cut(s) 395, 653, 2555, 2704
BseXI GCAGC 5 cut(s) 400, 481, 1297, 1972, 1975
BseYI CCCAGC 3 cut(s) 503, 2026, 2245
BsgI GTGCAG 1 cut(s) 520
Bsh1285I CGRYCG 1 cut(s) 2638
BshNI GGYRCC 2 cut(s) 521, 1091
BsiEI CGRYCG 1 cut(s) 2638
BsiHKAI GWGCWC 2 cut(s) 651, 1762
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 4 cut(s) 206, 896, 1044, 1495
BslFI GGGAC 2 cut(s) 935, 2228
BsmAI GTCTC 1 cut(s) 726
BsmFI GGGAC 2 cut(s) 935, 2228
Bso31I GGTCTC 1 cut(s) 726
BsoBI CYCGRG 1 cut(s) 461
Bsp119I TTCGAA 3 cut(s) 585, 1995, 2115
Bsp1286I GDGCHC 2 cut(s) 651, 1762
Bsp1407I TGTACA 1 cut(s) 789
BspACI CCGC 6 cut(s) 5, 24, 31, 375, 1313, 2486
BspCNI CTCAG 4 cut(s) 420, 556, 829, 1851
BspHI TCATGA 1 cut(s) 2230
BspLI GGNNCC 6 cut(s) 523, 843, 1093, 1693, 2084, 2244
BspOI GCTAGC 1 cut(s) 782
BspPI GGATC 4 cut(s) 78, 1686, 1699, 2433
BspQI GCTCTTC 1 cut(s) 2541
BspT104I TTCGAA 3 cut(s) 585, 1995, 2115
BspT107I GGYRCC 2 cut(s) 521, 1091
BspTNI GGTCTC 1 cut(s) 726
BsrBI CCGCTC 1 cut(s) 2486
BsrDI GCAATG 2 cut(s) 498, 2080
BsrGI TGTACA 1 cut(s) 789
BsrI ACTGG 6 cut(s) 341, 674, 1338, 1954, 2016, 2051
BssECI CCNNGG 4 cut(s) 300, 460, 889, 1399
BssNAI GTATAC 1 cut(s) 1238
BssSI CACGAG 2 cut(s) 969, 2215
BssT1I CCWWGG 1 cut(s) 889
Bst1107I GTATAC 1 cut(s) 1238
Bst2BI CACGAG 2 cut(s) 969, 2215
Bst2UI CCWGG 8 cut(s) 292, 301, 379, 1190, 1372, 1401, 1521, 2240
Bst4CI ACNGT 9 cut(s) 12, 83, 124, 215, 329, 789, 814, 1669, 1780
Bst6I CTCTTC 2 cut(s) 1554, 2541
BstAPI GCANNNNNTGC 1 cut(s) 911
BstAUI TGTACA 1 cut(s) 789
BstBAI YACGTR 1 cut(s) 78
BstBI TTCGAA 3 cut(s) 585, 1995, 2115
BstC8I GCNNGC 2 cut(s) 780, 2659
BstDEI CTNAG 9 cut(s) 147, 407, 543, 763, 837, 1052, 1096, 1859, 2255
BstENI CCTNNNNNAGG 2 cut(s) 1288, 2258
BstF5I GGATG 6 cut(s) 190, 351, 437, 471, 1167, 1300
BstH2I RGCGCY 3 cut(s) 385, 414, 1465
BstHHI GCGC 4 cut(s) 384, 413, 1464, 2348
BstMAI GTCTC 1 cut(s) 726
BstMCI CGRYCG 1 cut(s) 2638
BstMWI GCNNNNNNNGC 5 cut(s) 500, 850, 911, 2047, 2162
BstNI CCWGG 8 cut(s) 292, 301, 379, 1190, 1372, 1401, 1521, 2240
BstSFI CTRYAG 1 cut(s) 676
BstV1I GCAGC 5 cut(s) 400, 481, 1297, 1972, 1975
BstV2I GAAGAC 3 cut(s) 473, 679, 1658
BstX2I RGATCY 3 cut(s) 1144, 1691, 1844
BstXI CCANNNNNNTGG 1 cut(s) 448
BstYI RGATCY 3 cut(s) 1144, 1691, 1844
BstZ17I GTATAC 1 cut(s) 1238
Bsu36I CCTNAGG 2 cut(s) 763, 1052
BsuI GTATCC 1 cut(s) 1050
BtgZI GCGATG 1 cut(s) 1995
BtrI CACGTC 1 cut(s) 2200
BtsCI GGATG 6 cut(s) 190, 351, 437, 471, 1167, 1300
BtsI GCAGTG 2 cut(s) 26, 537
BtsIMutI CAGTG 7 cut(s) 26, 211, 334, 537, 785, 810, 1776
Cac8I GCNNGC 2 cut(s) 780, 2659
CaiI CAGNNNCTG 1 cut(s) 1673
CciI TCATGA 1 cut(s) 2230
CfoI GCGC 4 cut(s) 384, 413, 1464, 2348
CseI GACGC 1 cut(s) 1027
CsiI ACCWGGT 1 cut(s) 1370
Csp6I GTAC 5 cut(s) 79, 695, 790, 1298, 2522
CviQI GTAC 5 cut(s) 79, 695, 790, 1298, 2522
DdeI CTNAG 9 cut(s) 147, 407, 543, 763, 837, 1052, 1096, 1859, 2255
DraI TTTAAA 1 cut(s) 1635
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 3 cut(s) 272, 1723, 2236
Eam1104I CTCTTC 2 cut(s) 1554, 2541
EarI CTCTTC 2 cut(s) 1554, 2541
EciI GGCGGA 2 cut(s) 20, 364
Ecl136II GAGCTC 1 cut(s) 1760
Eco130I CCWWGG 1 cut(s) 889
Eco147I AGGCCT 1 cut(s) 514
Eco24I GRGCYC 1 cut(s) 1762
Eco31I GGTCTC 1 cut(s) 726
Eco47I GGWCC 5 cut(s) 134, 208, 1073, 2082, 2242
Eco47III AGCGCT 1 cut(s) 412
Eco53kI GAGCTC 1 cut(s) 1760
Eco57I CTGAAG 5 cut(s) 684, 1825, 1932, 1950, 2187
Eco81I CCTNAGG 2 cut(s) 763, 1052
Eco88I CYCGRG 1 cut(s) 461
EcoICRI GAGCTC 1 cut(s) 1760
EcoNI CCTNNNNNAGG 2 cut(s) 1288, 2258
EcoO109I RGGNCCY 2 cut(s) 2082, 2242
EcoRI GAATTC 1 cut(s) 1245
EcoRII CCWGG 8 cut(s) 290, 299, 377, 1188, 1370, 1399, 1519, 2238
EcoT14I CCWWGG 1 cut(s) 889
EcoT22I ATGCAT 1 cut(s) 1485
EcoT38I GRGCYC 1 cut(s) 1762
ErhI CCWWGG 1 cut(s) 889
FaqI GGGAC 2 cut(s) 935, 2228
FbaI TGATCA 2 cut(s) 331, 2173
FblI GTMKAC 2 cut(s) 1174, 1237
Fnu4HI GCNGC 5 cut(s) 414, 495, 1311, 1986, 1989
FokI GGATG 6 cut(s) 197, 338, 424, 478, 1174, 1307
FriOI GRGCYC 1 cut(s) 1762
Fsp4HI GCNGC 5 cut(s) 414, 495, 1311, 1986, 1989
FspBI CTAG 6 cut(s) 779, 890, 1511, 1644, 2066, 2564
GlaI GCGC 4 cut(s) 383, 412, 1463, 2347
GluI GCNGC 5 cut(s) 414, 495, 1311, 1986, 1989
GsaI CCCAGC 3 cut(s) 507, 2030, 2249
GsuI CTGGAG 1 cut(s) 1776
HaeII RGCGCY 3 cut(s) 385, 414, 1465
HapII CCGG 4 cut(s) 206, 896, 1044, 1495
HgaI GACGC 1 cut(s) 1027
HhaI GCGC 4 cut(s) 384, 413, 1464, 2348
Hin6I GCGC 4 cut(s) 382, 411, 1462, 2346
HinP1I GCGC 4 cut(s) 382, 411, 1462, 2346
HincII GTYRAC 4 cut(s) 817, 939, 1783, 2382
HindII GTYRAC 4 cut(s) 817, 939, 1783, 2382
HindIII AAGCTT 2 cut(s) 149, 2154
HinfI GANTC 7 cut(s) 92, 478, 1185, 1603, 1772, 2206, 2696
HpaII CCGG 4 cut(s) 206, 896, 1044, 1495
HphI GGTGA 6 cut(s) 109, 341, 779, 1360, 1568, 2215
Hpy166II GTNNAC 9 cut(s) 211, 695, 817, 939, 1175, 1238, 1783, 1967, 2382
Hpy188I TCNGA 9 cut(s) 16, 253, 1084, 1322, 1390, 1844, 1867, 1888, 2647
Hpy8I GTNNAC 9 cut(s) 211, 695, 817, 939, 1175, 1238, 1783, 1967, 2382
Hpy99I CGWCG 3 cut(s) 2201, 2409, 2709
HpyAV CCTTC 9 cut(s) 201, 337, 708, 1029, 1547, 1849, 1925, 2002, 2444
HpyCH4III ACNGT 9 cut(s) 12, 83, 124, 215, 329, 789, 814, 1669, 1780
HpyCH4IV ACGT 6 cut(s) 77, 489, 986, 2199, 2384, 2704
HpyF10VI GCNNNNNNNGC 5 cut(s) 500, 850, 911, 2047, 2162
HpyF3I CTNAG 9 cut(s) 147, 407, 543, 763, 837, 1052, 1096, 1859, 2255
HpySE526I ACGT 6 cut(s) 77, 489, 986, 2199, 2384, 2704
HspAI GCGC 4 cut(s) 382, 411, 1462, 2346
Ksp22I TGATCA 2 cut(s) 331, 2173
LguI GCTCTTC 1 cut(s) 2541
LmnI GCTCC 2 cut(s) 1757, 2335
Lsp1109I GCAGC 5 cut(s) 400, 481, 1297, 1972, 1975
LweI GCATC 5 cut(s) 303, 751, 1972, 2302, 2450
MabI ACCWGGT 1 cut(s) 1370
MaeI CTAG 6 cut(s) 779, 890, 1511, 1644, 2066, 2564
MaeII ACGT 6 cut(s) 77, 489, 986, 2199, 2384, 2704
MaeIII GTNAC 6 cut(s) 113, 617, 867, 1663, 1877, 2693
MbiI CCGCTC 1 cut(s) 2486
MflI RGATCY 3 cut(s) 1144, 1691, 1844
MhlI GDGCHC 2 cut(s) 651, 1762
MlsI TGGCCA 2 cut(s) 274, 2238
MluNI TGGCCA 2 cut(s) 274, 2238
MlyI GAGTC 3 cut(s) 487, 1194, 2690
MmeI TCCRAC 5 cut(s) 231, 465, 578, 995, 1100
Mox20I TGGCCA 2 cut(s) 274, 2238
Mph1103I ATGCAT 1 cut(s) 1485
MroXI GAANNNNTTC 4 cut(s) 1061, 1214, 1361, 2111
MscI TGGCCA 2 cut(s) 274, 2238
MslI CAYNNNNRTG 2 cut(s) 615, 1791
Msp20I TGGCCA 2 cut(s) 274, 2238
MspA1I CMGCKG 1 cut(s) 2431
MspI CCGG 4 cut(s) 206, 896, 1044, 1495
MvaI CCWGG 8 cut(s) 292, 301, 379, 1190, 1372, 1401, 1521, 2240
MwoI GCNNNNNNNGC 5 cut(s) 500, 850, 911, 2047, 2162
NciI CCSGG 3 cut(s) 896, 1044, 1496
NheI GCTAGC 1 cut(s) 778
NlaIV GGNNCC 6 cut(s) 523, 843, 1093, 1693, 2084, 2244
NmuCI GTSAC 5 cut(s) 113, 617, 1663, 1877, 2693
NsiI ATGCAT 1 cut(s) 1485
NspV TTCGAA 3 cut(s) 585, 1995, 2115
PagI TCATGA 1 cut(s) 2230
PceI AGGCCT 1 cut(s) 514
PciSI GCTCTTC 1 cut(s) 2541
PcsI WCGNNNNNNNCGW 2 cut(s) 79, 123
PdmI GAANNNNTTC 4 cut(s) 1061, 1214, 1361, 2111
PfeI GAWTC 4 cut(s) 92, 1603, 1772, 2206
PflFI GACNNNGTC 1 cut(s) 947
PflMI CCANNNNNTGG 1 cut(s) 827
PfoI TCCNGGA 1 cut(s) 1494
PkrI GCNGC 5 cut(s) 415, 496, 1312, 1987, 1990
Ple19I CGATCG 1 cut(s) 2638
PleI GAGTC 3 cut(s) 486, 1193, 2690
PpsI GAGTC 3 cut(s) 486, 1193, 2690
Ppu21I YACGTR 1 cut(s) 78
PpuMI RGGWCCY 2 cut(s) 2082, 2242
Psp124BI GAGCTC 1 cut(s) 1762
Psp1406I AACGTT 1 cut(s) 986
Psp5II RGGWCCY 2 cut(s) 2082, 2242
Psp6I CCWGG 8 cut(s) 290, 299, 377, 1188, 1370, 1399, 1519, 2238
PspFI CCCAGC 3 cut(s) 503, 2026, 2245
PspGI CCWGG 8 cut(s) 290, 299, 377, 1188, 1370, 1399, 1519, 2238
PspN4I GGNNCC 6 cut(s) 523, 843, 1093, 1693, 2084, 2244
PspPPI RGGWCCY 2 cut(s) 2082, 2242
PstNI CAGNNNCTG 1 cut(s) 1673
PsuI RGATCY 3 cut(s) 1144, 1691, 1844
PsyI GACNNNGTC 1 cut(s) 947
PvuI CGATCG 1 cut(s) 2638
PvuII CAGCTG 1 cut(s) 2431
RsaI GTAC 5 cut(s) 80, 696, 791, 1299, 2523
RsaNI GTAC 5 cut(s) 79, 695, 790, 1298, 2522
RseI CAYNNNNRTG 2 cut(s) 615, 1791
SacI GAGCTC 1 cut(s) 1762
SapI GCTCTTC 1 cut(s) 2541
SatI GCNGC 5 cut(s) 414, 495, 1311, 1986, 1989
SchI GAGTC 3 cut(s) 487, 1194, 2690
SduI GDGCHC 2 cut(s) 651, 1762
SexAI ACCWGGT 1 cut(s) 1370
SfaNI GCATC 5 cut(s) 303, 751, 1972, 2302, 2450
SfcI CTRYAG 1 cut(s) 676
SfuI TTCGAA 3 cut(s) 585, 1995, 2115
SinI GGWCC 5 cut(s) 134, 208, 1073, 2082, 2242
SmiMI CAYNNNNRTG 2 cut(s) 615, 1791
SmlI CTYRAG 3 cut(s) 179, 1659, 2089
SmoI CTYRAG 3 cut(s) 179, 1659, 2089
SseBI AGGCCT 1 cut(s) 514
SsiI CCGC 6 cut(s) 5, 24, 31, 375, 1313, 2486
SspI AATATT 1 cut(s) 2575
SspMI CTAG 6 cut(s) 779, 890, 1511, 1644, 2066, 2564
SstI GAGCTC 1 cut(s) 1762
StuI AGGCCT 1 cut(s) 514
StyI CCWWGG 1 cut(s) 889
TaaI ACNGT 9 cut(s) 12, 83, 124, 215, 329, 789, 814, 1669, 1780
TaiI ACGT 6 cut(s) 80, 492, 989, 2202, 2387, 2707
TatI WGTACW 2 cut(s) 789, 1297
TfiI GAWTC 4 cut(s) 92, 1603, 1772, 2206
TscAI CASTG 7 cut(s) 33, 218, 341, 544, 792, 817, 1783
TseFI GTSAC 5 cut(s) 113, 617, 1663, 1877, 2693
TseI GCWGC 5 cut(s) 413, 494, 1310, 1985, 1988
Tsp45I GTSAC 5 cut(s) 113, 617, 1663, 1877, 2693
TspGWI ACGGA 1 cut(s) 2176
TspRI CASTG 7 cut(s) 33, 218, 341, 544, 792, 817, 1783
Tth111I GACNNNGTC 1 cut(s) 947
Van91I CCANNNNNTGG 1 cut(s) 827
VpaK11BI GGWCC 5 cut(s) 134, 208, 1073, 2082, 2242
XagI CCTNNNNNAGG 2 cut(s) 1288, 2258
XapI RAATTY 7 cut(s) 231, 1196, 1245, 1333, 2368, 2493, 2588
XbaI TCTAGA 1 cut(s) 1510
XcmI CCANNNNNNNNNTGG 1 cut(s) 267
XmaJI CCTAGG 1 cut(s) 889
XmiI GTMKAC 2 cut(s) 1174, 1237
XmnI GAANNNNTTC 4 cut(s) 1061, 1214, 1361, 2111
XspI CTAG 6 cut(s) 779, 890, 1511, 1644, 2066, 2564
Zsp2I ATGCAT 1 cut(s) 1485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.