RLG00000033935

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016792
Physical Location & Seq
Reverse (-)
34364545 .. 34380352
15808 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033935

Sequence Viewer

Length: 1203 bp
ATGGCTTGGAGTACCGATGCAGCTGCCGGTTCTTCCCCATCTTCTCCAATCCCCATTTCATACAAATATGATGTGTTTCTGAATTTTAGTGGTGAAGACACGCGCACTACTTTTACAAGCCATTTGTACACAGCTTTGCGCAACAAAGGAATCAAGACCTTCATAGACGACGGGCTCAAAAGAGGAGAAGAAATATCTCGAGCACTTTTCGAAGCAATCAATGAGTCAAGGATGTCTGTCATCGTGTTCTCTGAAAACTACGCAGAATCACCTTGGTGTTTGGACGAACTTGTTGAGATCCTTGCATGTAGAGAATCAAAAGGACATATGGTTAGGCCAATTTTTTATAAAGTGAAACCCTCTGATGTACGACACCTGAGAGGTACTTTTGGGAAGGCACTTGCCCAACACGAACTCGATTTTGAGCATGATATTAAAATGGTGCGCAGATGGGGTGCAGCTCTTTCTGAAGCGGCGAATTTGTCCGGATGGCCCTTCAAGGATCAGGATGAATCTCGATTTATCAAACGCATCGTTAAAGAGATCGCAGGTCAAATATCAAACCCCAAGTTTTTCCTGGATGAATTTAGGGATCCACTTGCGGTAACAGCTATAGATCTTATTCCAAGACCACCACCTAGTGTGCTGCAAGTCACTTGGTCCCGTCCAGCTGAGGGTCATCTTAAGATAAATTATGACGGTGCCTATACTGTGAGGAAGGGAGGTCTAGGTGTGGTTGCTCGGGATACAAAAGGAAGTTTTGTGGCAGCAAAGTGCAGGTTGGTTAATGTTAAATCGGAGGACGATGTGGTGGCAATGGCAGCACTGGAGGCTTTGTGTCTGGCTAAGGAACTTGATTGGCCGCGAGTTTGTTTTGAGGGTGACTCTGTACGTGTTGTGAATGCTCTGCAAACTTCTACAGCTTCTGATGATTCTGACACATCCTCCTTCTGCTCCTCCCCTTCCTCCTCCTCCTCCTCCTCCTCCTCCTGCTTATTCGGTCAGATCATTAGTGCGATTCAATATATGTCACGCAGCTTTCAGCAGACTACATTTTCTCTTGTTCACCAGAAGGCTAATGTTGTTGCTGAGACCCTTGCTACACATGCTTTATCAAACCCCGAAGACAGACATTGGCTTGTTGAGCCTCCTACTTTTATCGACTCCTCTTTGGGTATTGAAAAATATACTTCTTCTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

401

Amino Acids

44.29

Weight (kDa)

5.91

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 183 5.8e-53 TIR domain
TIR_2 PF13676 25 - 120 3e-15 TIR domain
RVT_3 PF13456 231 - 319 1.5e-08 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 348
Acc16I TGCGCA 2 cut(s) 140, 446
Acc36I ACCTGC 2 cut(s) 539, 768
AccB1I GGYRCC 1 cut(s) 701
AccII CGCG 2 cut(s) 103, 865
AccIII TCCGGA 1 cut(s) 485
AciI CCGC 3 cut(s) 473, 602, 863
AclWI GGATC 4 cut(s) 292, 510, 587, 600
AcoI YGGCCR 1 cut(s) 860
AcsI RAATTY 3 cut(s) 82, 478, 584
AcuI CTGAAG 1 cut(s) 489
AdeI CACNNNGTG 1 cut(s) 641
AfaI GTAC 5 cut(s) 13, 128, 369, 385, 891
AfiI CCNNNNNNNGG 1 cut(s) 674
AflII CTTAAG 1 cut(s) 683
AflIII ACRYGT 1 cut(s) 892
AgsI TTSAA 3 cut(s) 499, 1022, 1181
AjnI CCWGG 1 cut(s) 576
AjuI GAANNNNNNNTTGG 2 cut(s) 40, 72
AleI CACNNNNGTG 1 cut(s) 274
AluBI AGCT 7 cut(s) 23, 134, 461, 611, 671, 923, 1038
AluI AGCT 7 cut(s) 23, 134, 461, 611, 671, 923, 1038
Alw21I GWGCWC 1 cut(s) 205
Alw26I GTCTC 1 cut(s) 1085
AlwI GGATC 4 cut(s) 292, 510, 587, 600
AlwNI CAGNNNCTG 1 cut(s) 926
Ama87I CYCGRG 2 cut(s) 198, 741
Aor13HI TCCGGA 1 cut(s) 485
AoxI GGCC 3 cut(s) 335, 491, 860
ApeKI GCWGC 7 cut(s) 20, 23, 458, 646, 767, 821, 1035
ApoI RAATTY 3 cut(s) 82, 478, 584
AspLEI GCGC 3 cut(s) 105, 141, 447
AspS9I GGNCC 2 cut(s) 492, 660
AsuHPI GGTGA 4 cut(s) 104, 261, 893, 1058
AsuII TTCGAA 1 cut(s) 210
AvaI CYCGRG 2 cut(s) 198, 741
AvaII GGWCC 1 cut(s) 660
BamHI GGATCC 1 cut(s) 592
BanI GGYRCC 1 cut(s) 701
BanII GRGCYC 1 cut(s) 177
BbsI GAAGAC 2 cut(s) 102, 1131
Bbv12I GWGCWC 1 cut(s) 205
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 7 cut(s) 10, 32, 470, 633, 779, 833, 1047
BccI CCATC 3 cut(s) 46, 444, 483
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 1 cut(s) 578
BciVI GTATCC 1 cut(s) 739
BcoDI GTCTC 1 cut(s) 1085
BfaI CTAG 2 cut(s) 639, 728
BfmI CTRYAG 2 cut(s) 612, 918
BfrI CTTAAG 1 cut(s) 683
BfuAI ACCTGC 2 cut(s) 539, 768
BfuI GTATCC 1 cut(s) 739
BglII AGATCT 1 cut(s) 616
BisI GCNGC 9 cut(s) 21, 24, 459, 474, 647, 768, 822, 863, 1036
BlsI GCNGC 9 cut(s) 22, 25, 460, 475, 648, 769, 823, 864, 1037
Bme1390I CCNGG 1 cut(s) 578
Bme18I GGWCC 1 cut(s) 660
BmeT110I CYCGRG 2 cut(s) 198, 741
BmgT120I GGNCC 2 cut(s) 492, 660
BmiI GGNNCC 3 cut(s) 594, 662, 703
BmrFI CCNGG 1 cut(s) 578
BmsI GCATC 2 cut(s) 7, 540
BpiI GAAGAC 2 cut(s) 102, 1131
BplI GAGNNNNNCTC 2 cut(s) 869, 901
BpmI CTGGAG 1 cut(s) 848
Bpu10I CCTNAGC 2 cut(s) 672, 846
Bpu14I TTCGAA 1 cut(s) 210
BsaAI YACGTR 1 cut(s) 893
BsaI GGTCTC 1 cut(s) 1085
BsaJI CCNNGG 1 cut(s) 272
BsaWI WCCGGW 1 cut(s) 485
BsaXI ACNNNNNCTCC 4 cut(s) 821, 851, 929, 959
Bsc4I CCNNNNNNNGG 1 cut(s) 674
Bse118I RCCGGY 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 831
Bse3DI GCAATG 1 cut(s) 822
BseAI TCCGGA 1 cut(s) 485
BseBI CCWGG 1 cut(s) 578
BseDI CCNNGG 1 cut(s) 272
BseGI GGATG 5 cut(s) 237, 494, 514, 586, 941
BseLI CCNNNNNNNGG 1 cut(s) 674
BseMI GCAATG 1 cut(s) 822
BseMII CTCAG 3 cut(s) 368, 663, 1080
BseNI ACTGG 1 cut(s) 831
BseXI GCAGC 7 cut(s) 10, 32, 470, 633, 779, 833, 1047
BsgI GTGCAG 2 cut(s) 477, 796
Bsh1236I CGCG 2 cut(s) 103, 865
BshFI GGCC 3 cut(s) 337, 493, 862
BshNI GGYRCC 1 cut(s) 701
BsiHKAI GWGCWC 1 cut(s) 205
BsiHKCI CYCGRG 2 cut(s) 198, 741
BsiSI CCGG 2 cut(s) 27, 486
BslFI GGGAC 1 cut(s) 646
BslI CCNNNNNNNGG 1 cut(s) 674
BsmAI GTCTC 1 cut(s) 1085
BsmFI GGGAC 1 cut(s) 646
BsmI GAATGC 1 cut(s) 907
BsnI GGCC 3 cut(s) 337, 493, 862
Bso31I GGTCTC 1 cut(s) 1085
BsoBI CYCGRG 2 cut(s) 198, 741
Bsp119I TTCGAA 1 cut(s) 210
Bsp1286I GDGCHC 2 cut(s) 177, 205
Bsp13I TCCGGA 1 cut(s) 485
Bsp1407I TGTACA 1 cut(s) 126
Bsp143I GATC 6 cut(s) 297, 502, 543, 592, 616, 1005
BspACI CCGC 3 cut(s) 473, 602, 863
BspANI GGCC 3 cut(s) 337, 493, 862
BspCNI CTCAG 3 cut(s) 369, 664, 1081
BspEI TCCGGA 1 cut(s) 485
BspFNI CGCG 2 cut(s) 103, 865
BspLI GGNNCC 3 cut(s) 594, 662, 703
BspMI ACCTGC 2 cut(s) 539, 768
BspPI GGATC 4 cut(s) 292, 510, 587, 600
BspT104I TTCGAA 1 cut(s) 210
BspT107I GGYRCC 1 cut(s) 701
BspTI CTTAAG 1 cut(s) 683
BspTNI GGTCTC 1 cut(s) 1085
BsrDI GCAATG 1 cut(s) 822
BsrFI RCCGGY 1 cut(s) 26
BsrGI TGTACA 1 cut(s) 126
BsrI ACTGG 1 cut(s) 831
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 1 cut(s) 272
BssMI GATC 6 cut(s) 297, 502, 543, 592, 616, 1005
BssT1I CCWWGG 1 cut(s) 272
Bst2UI CCWGG 1 cut(s) 578
Bst4CI ACNGT 2 cut(s) 701, 712
BstAFI CTTAAG 1 cut(s) 683
BstAUI TGTACA 1 cut(s) 126
BstBAI YACGTR 1 cut(s) 893
BstBI TTCGAA 1 cut(s) 210
BstDEI CTNAG 4 cut(s) 377, 672, 846, 1089
BstF5I GGATG 5 cut(s) 237, 494, 514, 586, 941
BstFNI CGCG 2 cut(s) 103, 865
BstHHI GCGC 3 cut(s) 105, 141, 447
BstKTI GATC 6 cut(s) 300, 505, 546, 595, 619, 1008
BstMAI GTCTC 1 cut(s) 1085
BstMBI GATC 6 cut(s) 297, 502, 543, 592, 616, 1005
BstMWI GCNNNNNNNGC 5 cut(s) 608, 821, 830, 1106, 1144
BstNI CCWGG 1 cut(s) 578
BstNSI RCATGY 2 cut(s) 309, 1109
BstSCI CCNGG 1 cut(s) 576
BstSFI CTRYAG 2 cut(s) 612, 918
BstUI CGCG 2 cut(s) 103, 865
BstV1I GCAGC 7 cut(s) 10, 32, 470, 633, 779, 833, 1047
BstV2I GAAGAC 2 cut(s) 102, 1131
BstX2I RGATCY 3 cut(s) 297, 592, 616
BstYI RGATCY 3 cut(s) 297, 592, 616
BsuI GTATCC 1 cut(s) 739
BsuRI GGCC 3 cut(s) 337, 493, 862
BtsCI GGATG 5 cut(s) 237, 494, 514, 586, 941
BtsIMutI CAGTG 1 cut(s) 824
BveI ACCTGC 2 cut(s) 539, 768
CaiI CAGNNNCTG 1 cut(s) 926
CfoI GCGC 3 cut(s) 105, 141, 447
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 2 cut(s) 492, 660
Csp6I GTAC 5 cut(s) 12, 127, 368, 384, 890
CviAII CATG 3 cut(s) 306, 428, 1106
CviQI GTAC 5 cut(s) 12, 127, 368, 384, 890
DdeI CTNAG 4 cut(s) 377, 672, 846, 1089
DpnI GATC 6 cut(s) 299, 504, 545, 594, 618, 1007
DpnII GATC 6 cut(s) 297, 502, 543, 592, 616, 1005
DraIII CACNNNGTG 1 cut(s) 641
EaeI YGGCCR 1 cut(s) 860
Eco130I CCWWGG 1 cut(s) 272
Eco24I GRGCYC 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 1085
Eco47I GGWCC 1 cut(s) 660
Eco57I CTGAAG 1 cut(s) 489
Eco88I CYCGRG 2 cut(s) 198, 741
EcoRII CCWGG 1 cut(s) 576
EcoT14I CCWWGG 1 cut(s) 272
EcoT38I GRGCYC 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 272
FaeI CATG 3 cut(s) 309, 431, 1109
FaqI GGGAC 1 cut(s) 646
FatI CATG 3 cut(s) 305, 427, 1105
FauNDI CATATG 1 cut(s) 327
Fnu4HI GCNGC 9 cut(s) 21, 24, 459, 474, 647, 768, 822, 863, 1036
FokI GGATG 5 cut(s) 244, 501, 521, 593, 928
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 9 cut(s) 21, 24, 459, 474, 647, 768, 822, 863, 1036
FspBI CTAG 2 cut(s) 639, 728
FspI TGCGCA 2 cut(s) 140, 446
GlaI GCGC 3 cut(s) 104, 140, 446
GluI GCNGC 9 cut(s) 21, 24, 459, 474, 647, 768, 822, 863, 1036
GsuI CTGGAG 1 cut(s) 848
HaeIII GGCC 3 cut(s) 337, 493, 862
HapII CCGG 2 cut(s) 27, 486
HhaI GCGC 3 cut(s) 105, 141, 447
Hin1II CATG 3 cut(s) 309, 431, 1109
Hin6I GCGC 3 cut(s) 103, 139, 445
HinP1I GCGC 3 cut(s) 103, 139, 445
HinfI GANTC 9 cut(s) 150, 224, 266, 314, 512, 884, 932, 1018, 1163
HpaII CCGG 2 cut(s) 27, 486
HphI GGTGA 4 cut(s) 104, 261, 893, 1058
Hpy166II GTNNAC 2 cut(s) 129, 1066
Hpy188I TCNGA 8 cut(s) 81, 253, 364, 469, 799, 928, 937, 1005
Hpy188III TCNNGA 6 cut(s) 154, 198, 486, 506, 516, 743
Hpy8I GTNNAC 2 cut(s) 129, 1066
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 7 cut(s) 169, 388, 505, 712, 958, 972, 1066
HpyCH4III ACNGT 2 cut(s) 701, 712
HpyCH4IV ACGT 1 cut(s) 892
HpyCH4V TGCA 6 cut(s) 20, 305, 458, 649, 777, 910
HpyF10VI GCNNNNNNNGC 5 cut(s) 608, 821, 830, 1106, 1144
HpyF3I CTNAG 4 cut(s) 377, 672, 846, 1089
HpySE526I ACGT 1 cut(s) 892
Hsp92II CATG 3 cut(s) 309, 431, 1109
HspAI GCGC 3 cut(s) 103, 139, 445
Kpn2I TCCGGA 1 cut(s) 485
Kzo9I GATC 6 cut(s) 297, 502, 543, 592, 616, 1005
LmnI GCTCC 1 cut(s) 959
Lsp1109I GCAGC 7 cut(s) 10, 32, 470, 633, 779, 833, 1047
LweI GCATC 2 cut(s) 7, 540
MaeI CTAG 2 cut(s) 639, 728
MaeII ACGT 1 cut(s) 892
MaeIII GTNAC 4 cut(s) 604, 652, 881, 1029
MalI GATC 6 cut(s) 299, 504, 545, 594, 618, 1007
MboI GATC 6 cut(s) 297, 502, 543, 592, 616, 1005
MboII GAAGA 6 cut(s) 24, 33, 107, 200, 1136, 1185
MflI RGATCY 3 cut(s) 297, 592, 616
MhlI GDGCHC 2 cut(s) 177, 205
MluCI AATT 5 cut(s) 82, 339, 478, 584, 691
MlyI GAGTC 3 cut(s) 233, 878, 1157
MroI TCCGGA 1 cut(s) 485
MseI TTAA 5 cut(s) 435, 537, 684, 786, 792
MslI CAYNNNNRTG 1 cut(s) 274
MspA1I CMGCKG 2 cut(s) 23, 671
MspCI CTTAAG 1 cut(s) 683
MspI CCGG 2 cut(s) 27, 486
MspR9I CCNGG 1 cut(s) 578
Mva1269I GAATGC 1 cut(s) 907
MvaI CCWGG 1 cut(s) 578
MvnI CGCG 2 cut(s) 103, 865
MwoI GCNNNNNNNGC 5 cut(s) 608, 821, 830, 1106, 1144
NdeI CATATG 1 cut(s) 327
NdeII GATC 6 cut(s) 297, 502, 543, 592, 616, 1005
NlaIII CATG 3 cut(s) 309, 431, 1109
NlaIV GGNNCC 3 cut(s) 594, 662, 703
NmuCI GTSAC 3 cut(s) 652, 881, 1029
NsbI TGCGCA 2 cut(s) 140, 446
NspI RCATGY 2 cut(s) 309, 1109
NspV TTCGAA 1 cut(s) 210
OliI CACNNNNGTG 1 cut(s) 274
PaeR7I CTCGAG 1 cut(s) 198
PctI GAATGC 1 cut(s) 907
PfeI GAWTC 6 cut(s) 150, 266, 314, 512, 932, 1018
PfoI TCCNGGA 1 cut(s) 576
PkrI GCNGC 9 cut(s) 22, 25, 460, 475, 648, 769, 823, 864, 1037
PleI GAGTC 3 cut(s) 232, 878, 1157
PpsI GAGTC 3 cut(s) 232, 878, 1157
Ppu21I YACGTR 1 cut(s) 893
PsiI TTATAA 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 576
PspGI CCWGG 1 cut(s) 576
PspN4I GGNNCC 3 cut(s) 594, 662, 703
PspPI GGNCC 2 cut(s) 492, 660
PstNI CAGNNNCTG 1 cut(s) 926
PsuI RGATCY 3 cut(s) 297, 592, 616
PvuII CAGCTG 2 cut(s) 23, 671
RsaI GTAC 5 cut(s) 13, 128, 369, 385, 891
RsaNI GTAC 5 cut(s) 12, 127, 368, 384, 890
RseI CAYNNNNRTG 1 cut(s) 274
SaqAI TTAA 5 cut(s) 435, 537, 684, 786, 792
SatI GCNGC 9 cut(s) 21, 24, 459, 474, 647, 768, 822, 863, 1036
Sau3AI GATC 6 cut(s) 297, 502, 543, 592, 616, 1005
Sau96I GGNCC 2 cut(s) 492, 660
SchI GAGTC 3 cut(s) 233, 878, 1157
ScrFI CCNGG 1 cut(s) 578
SduI GDGCHC 2 cut(s) 177, 205
SfaNI GCATC 2 cut(s) 7, 540
SfcI CTRYAG 2 cut(s) 612, 918
Sfr274I CTCGAG 1 cut(s) 198
SfuI TTCGAA 1 cut(s) 210
SinI GGWCC 1 cut(s) 660
SlaI CTCGAG 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 274
SmlI CTYRAG 2 cut(s) 198, 683
SmoI CTYRAG 2 cut(s) 198, 683
Sse9I AATT 5 cut(s) 82, 339, 478, 584, 691
SsiI CCGC 3 cut(s) 473, 602, 863
SspMI CTAG 2 cut(s) 639, 728
StyD4I CCNGG 1 cut(s) 576
StyI CCWWGG 1 cut(s) 272
TaaI ACNGT 2 cut(s) 701, 712
TaiI ACGT 1 cut(s) 895
TaqI TCGA 5 cut(s) 199, 210, 417, 517, 1161
TaqII GACCGA 1 cut(s) 989
TasI AATT 5 cut(s) 82, 339, 478, 584, 691
TatI WGTACW 1 cut(s) 126
TauI GCSGC 2 cut(s) 476, 865
TfiI GAWTC 6 cut(s) 150, 266, 314, 512, 932, 1018
Tru1I TTAA 5 cut(s) 435, 537, 684, 786, 792
Tru9I TTAA 5 cut(s) 435, 537, 684, 786, 792
TscAI CASTG 1 cut(s) 831
TseFI GTSAC 3 cut(s) 652, 881, 1029
TseI GCWGC 7 cut(s) 20, 23, 458, 646, 767, 821, 1035
Tsp45I GTSAC 3 cut(s) 652, 881, 1029
TspDTI ATGAA 4 cut(s) 48, 151, 525, 597
TspRI CASTG 1 cut(s) 831
Vha464I CTTAAG 1 cut(s) 683
VpaK11BI GGWCC 1 cut(s) 660
XapI RAATTY 3 cut(s) 82, 478, 584
XceI RCATGY 2 cut(s) 309, 1109
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XhoI CTCGAG 1 cut(s) 198
XspI CTAG 2 cut(s) 639, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.