Rw5G025030

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016792
Physical Location & Seq
Reverse (-)
35522460 .. 35528694
6235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G025030.1

Sequence Viewer

Length: 1215 bp
ATGGCTGGTAGAACTAATCAAGTAGCCTCTTCTTCTGGTGCTGGTTCTTCTCCTCCTTCTTCAGTCCCCATGTCCTACAAACACGACGTGTTTCTGAGTTTTAGAGGTGAAGATACACGCTCGAATTTTACAGGGCATTTGTACACAGCTTTGCGCAACAAAGGAATCAAGACCTTCATAGACGATGGGCTCAAAAGAGGAGAAGACATATCTCGAGCACTTTTCCGAGCAATCAATGAATCAAGGATGTCTGTCATCGTGTTCTCTGAAAACTATGCAGAATCACCATGGTGTTTGGACGAGCTTGTTGAGATCCTTGGATGTAGAGAATCAAAAGGACAGATGGTTAGGCCAATTTTTTACAAAGTGAAACCCTCTGATGTACGACACCTGAGAGGTACTTTTGGCAAGGCACTTGCCCAACATGAACTCGATTTTGAGCATGATATTATGATTGTGCGTAGATGGGGTGCAGCTCTTTCTGAAGCGGCGAATTTGTCCGGGTGGCCCTTGAAGGATCAGAATGAATCTCGACTTATCAAACGCATCGTTAAAGAGATCGCAGATCAAGTAGGGCTGGACTCGGTATCTAACCCCAAGTTGTCCCTGGATGAAATCAGGGATCCACTTGTGGTAACAGCTATAGATCTTATTCCAAGACCACCACCTAGTTTGCTGCAAGTCACTTGGTCCCGTCCAGCTGAGGGTTATCTTAAGATAAATTATGATGGCGCCTATACTGTATGGAAGGGAGGTTTAGGTGTGGTTGCTCGGGATACGAAGGGACGTTTTGTGGCAGCAAAGTGCAGGTTGGTTAATGTTGAATCAGAGGGCGATGTGGTGGCAATGGCAGCATTTGAGGCTCTGTGTCTAGCTAAGGAACTGGGTTGGCCACAAGTTTGCTTTGAGGGTGACTCCGTACGTGTTGTGAATGCTCTGCAAACTTCTACTGCTTCTGATGAGTCCGACACTTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCATTTGGTCAGATCATTAGTGCGATTCAATATATGTCACGCAGCTTTCAGCAGACTACATTTTCTCTTGTTCACCAGAAGGCTAATGTTGTTGCTGAGAACCTTGCTACACATGCTTTATCAAACCCCCTAGAGAAACATTGGATTGCTAAGCCCCCTACTTTTATCGACTCCTCTTTGGCTATTGAAAAATATGCTTCTTCTACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

404

Amino Acids

44.34

Weight (kDa)

6.73

Isoelectric Point (pI)

47.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 27 - 191 5.3e-52 TIR domain
TIR_2 PF13676 30 - 125 9.8e-16 TIR domain
RVT_3 PF13456 241 - 374 6.9e-12 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 155
Acc36I ACCTGC 1 cut(s) 798
AccB1I GGYRCC 1 cut(s) 731
AciI CCGC 1 cut(s) 488
AclWI GGATC 4 cut(s) 307, 525, 617, 630
AcoI YGGCCR 1 cut(s) 890
AcsI RAATTY 2 cut(s) 124, 493
AcuI CTGAAG 2 cut(s) 45, 504
AcyI GRCGYC 1 cut(s) 732
AdeI CACNNNGTG 1 cut(s) 88
AfaI GTAC 4 cut(s) 143, 384, 400, 921
AfiI CCNNNNNNNGG 1 cut(s) 704
AflII CTTAAG 1 cut(s) 713
AflIII ACRYGT 2 cut(s) 87, 922
AgsI TTSAA 4 cut(s) 514, 824, 1034, 1193
AjiI CACGTC 1 cut(s) 88
AjnI CCWGG 1 cut(s) 606
AleI CACNNNNGTG 1 cut(s) 289
AluBI AGCT 7 cut(s) 149, 304, 476, 641, 701, 875, 1050
AluI AGCT 7 cut(s) 149, 304, 476, 641, 701, 875, 1050
Alw21I GWGCWC 1 cut(s) 220
AlwI GGATC 4 cut(s) 307, 525, 617, 630
Ama87I CYCGRG 2 cut(s) 213, 771
AoxI GGCC 3 cut(s) 350, 506, 890
ApeKI GCWGC 5 cut(s) 473, 676, 797, 851, 1047
ApoI RAATTY 2 cut(s) 124, 493
AspLEI GCGC 2 cut(s) 156, 734
AspS9I GGNCC 2 cut(s) 507, 690
AsuC2I CCSGG 1 cut(s) 502
AsuHPI GGTGA 4 cut(s) 119, 276, 923, 1070
AvaI CYCGRG 2 cut(s) 213, 771
AvaII GGWCC 1 cut(s) 690
BalI TGGCCA 1 cut(s) 892
BamHI GGATCC 1 cut(s) 622
BanI GGYRCC 1 cut(s) 731
BanII GRGCYC 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 220
BbvCI CCTCAGC 1 cut(s) 702
BbvI GCAGC 5 cut(s) 485, 663, 809, 863, 1059
BccI CCATC 4 cut(s) 179, 337, 459, 722
BciT130I CCWGG 1 cut(s) 608
BciVI GTATCC 1 cut(s) 769
BcnI CCSGG 1 cut(s) 502
BfaI CTAG 3 cut(s) 669, 872, 1136
BfmI CTRYAG 1 cut(s) 642
BfoI RGCGCY 1 cut(s) 735
BfrI CTTAAG 1 cut(s) 713
BfuAI ACCTGC 1 cut(s) 798
BfuI GTATCC 1 cut(s) 769
BglII AGATCT 1 cut(s) 646
BisI GCNGC 6 cut(s) 474, 489, 677, 798, 852, 1048
BlpI GCTNAGC 1 cut(s) 1155
BlsI GCNGC 6 cut(s) 475, 490, 678, 799, 853, 1049
Bme1390I CCNGG 2 cut(s) 502, 608
Bme18I GGWCC 1 cut(s) 690
BmeT110I CYCGRG 2 cut(s) 213, 771
BmgBI CACGTC 1 cut(s) 88
BmgT120I GGNCC 2 cut(s) 507, 690
BmiI GGNNCC 3 cut(s) 624, 692, 733
BmrFI CCNGG 2 cut(s) 502, 608
BmrI ACTGGG 1 cut(s) 893
BmsI GCATC 1 cut(s) 555
BmuI ACTGGG 1 cut(s) 893
BpiI GAAGAC 1 cut(s) 210
BplI GAGNNNNNCTC 2 cut(s) 899, 931
Bpu10I CCTNAGC 2 cut(s) 702, 876
Bpu1102I GCTNAGC 1 cut(s) 1155
BpuMI CCSGG 1 cut(s) 502
BsaAI YACGTR 2 cut(s) 923, 1212
BsaHI GRCGYC 1 cut(s) 732
BsaJI CCNNGG 3 cut(s) 287, 316, 606
BsaXI ACNNNNNCTCC 2 cut(s) 959, 989
Bsc4I CCNNNNNNNGG 1 cut(s) 704
Bse1I ACTGG 1 cut(s) 888
Bse3DI GCAATG 1 cut(s) 852
BseBI CCWGG 1 cut(s) 608
BseDI CCNNGG 3 cut(s) 287, 316, 606
BseGI GGATG 3 cut(s) 252, 326, 616
BseLI CCNNNNNNNGG 1 cut(s) 704
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 4 cut(s) 86, 383, 693, 1092
BseNI ACTGG 1 cut(s) 888
BseXI GCAGC 5 cut(s) 485, 663, 809, 863, 1059
BsgI GTGCAG 2 cut(s) 492, 826
BshFI GGCC 3 cut(s) 352, 508, 892
BshNI GGYRCC 1 cut(s) 731
BsiHKAI GWGCWC 1 cut(s) 220
BsiHKCI CYCGRG 2 cut(s) 213, 771
BsiSI CCGG 1 cut(s) 501
BsiWI CGTACG 1 cut(s) 919
BslFI GGGAC 4 cut(s) 50, 589, 676, 798
BslI CCNNNNNNNGG 1 cut(s) 704
BsmFI GGGAC 4 cut(s) 50, 589, 676, 798
BsmI GAATGC 1 cut(s) 937
BsnI GGCC 3 cut(s) 352, 508, 892
BsoBI CYCGRG 2 cut(s) 213, 771
Bsp1286I GDGCHC 2 cut(s) 192, 220
Bsp1407I TGTACA 1 cut(s) 141
Bsp143I GATC 7 cut(s) 312, 517, 558, 565, 622, 646, 1017
Bsp1720I GCTNAGC 1 cut(s) 1155
Bsp19I CCATGG 1 cut(s) 287
BspACI CCGC 1 cut(s) 488
BspANI GGCC 3 cut(s) 352, 508, 892
BspCNI CTCAG 4 cut(s) 87, 384, 694, 1093
BspLI GGNNCC 3 cut(s) 624, 692, 733
BspMI ACCTGC 1 cut(s) 798
BspPI GGATC 4 cut(s) 307, 525, 617, 630
BspT107I GGYRCC 1 cut(s) 731
BspTI CTTAAG 1 cut(s) 713
BsrDI GCAATG 1 cut(s) 852
BsrGI TGTACA 1 cut(s) 141
BsrI ACTGG 1 cut(s) 888
BssECI CCNNGG 3 cut(s) 287, 316, 606
BssMI GATC 7 cut(s) 312, 517, 558, 565, 622, 646, 1017
BssNI GRCGYC 1 cut(s) 732
BssT1I CCWWGG 2 cut(s) 287, 316
Bst2UI CCWGG 1 cut(s) 608
Bst4CI ACNGT 1 cut(s) 742
Bst6I CTCTTC 1 cut(s) 34
BstACI GRCGYC 1 cut(s) 732
BstAFI CTTAAG 1 cut(s) 713
BstAUI TGTACA 1 cut(s) 141
BstBAI YACGTR 2 cut(s) 923, 1212
BstDEI CTNAG 6 cut(s) 95, 392, 702, 876, 1101, 1155
BstDSI CCRYGG 1 cut(s) 287
BstF5I GGATG 3 cut(s) 252, 326, 616
BstH2I RGCGCY 1 cut(s) 735
BstHHI GCGC 2 cut(s) 156, 734
BstKTI GATC 7 cut(s) 315, 520, 561, 568, 625, 649, 1020
BstMBI GATC 7 cut(s) 312, 517, 558, 565, 622, 646, 1017
BstMWI GCNNNNNNNGC 3 cut(s) 851, 860, 1118
BstNI CCWGG 1 cut(s) 608
BstNSI RCATGY 1 cut(s) 1121
BstSCI CCNGG 2 cut(s) 500, 606
BstSFI CTRYAG 1 cut(s) 642
BstSNI TACGTA 1 cut(s) 1212
BstV1I GCAGC 5 cut(s) 485, 663, 809, 863, 1059
BstV2I GAAGAC 1 cut(s) 210
BstX2I RGATCY 3 cut(s) 312, 622, 646
BstYI RGATCY 3 cut(s) 312, 622, 646
BsuI GTATCC 1 cut(s) 769
BsuRI GGCC 3 cut(s) 352, 508, 892
BtgI CCRYGG 1 cut(s) 287
BtgZI GCGATG 1 cut(s) 849
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 3 cut(s) 252, 326, 616
BveI ACCTGC 1 cut(s) 798
CfoI GCGC 2 cut(s) 156, 734
Cfr13I GGNCC 2 cut(s) 507, 690
Csp6I GTAC 4 cut(s) 142, 383, 399, 920
CspCI CAANNNNNGTGG 2 cut(s) 654, 689
CviAII CATG 5 cut(s) 70, 288, 425, 443, 1118
CviQI GTAC 4 cut(s) 142, 383, 399, 920
DdeI CTNAG 6 cut(s) 95, 392, 702, 876, 1101, 1155
DinI GGCGCC 1 cut(s) 733
DpnI GATC 7 cut(s) 314, 519, 560, 567, 624, 648, 1019
DpnII GATC 7 cut(s) 312, 517, 558, 565, 622, 646, 1017
DraIII CACNNNGTG 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 890
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
Eco105I TACGTA 1 cut(s) 1212
Eco130I CCWWGG 2 cut(s) 287, 316
Eco24I GRGCYC 1 cut(s) 192
Eco47I GGWCC 1 cut(s) 690
Eco57I CTGAAG 2 cut(s) 45, 504
Eco88I CYCGRG 2 cut(s) 213, 771
EcoRII CCWGG 1 cut(s) 606
EcoT14I CCWWGG 2 cut(s) 287, 316
EcoT38I GRGCYC 1 cut(s) 192
EgeI GGCGCC 1 cut(s) 733
EheI GGCGCC 1 cut(s) 733
ErhI CCWWGG 2 cut(s) 287, 316
FaeI CATG 5 cut(s) 73, 291, 428, 446, 1121
FaqI GGGAC 4 cut(s) 50, 589, 676, 798
FatI CATG 5 cut(s) 69, 287, 424, 442, 1117
Fnu4HI GCNGC 6 cut(s) 474, 489, 677, 798, 852, 1048
FokI GGATG 3 cut(s) 259, 333, 623
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 6 cut(s) 474, 489, 677, 798, 852, 1048
FspBI CTAG 3 cut(s) 669, 872, 1136
FspI TGCGCA 1 cut(s) 155
GlaI GCGC 2 cut(s) 155, 733
GluI GCNGC 6 cut(s) 474, 489, 677, 798, 852, 1048
HaeII RGCGCY 1 cut(s) 735
HaeIII GGCC 3 cut(s) 352, 508, 892
HapII CCGG 1 cut(s) 501
HhaI GCGC 2 cut(s) 156, 734
Hin1I GRCGYC 1 cut(s) 732
Hin1II CATG 5 cut(s) 73, 291, 428, 446, 1121
Hin6I GCGC 2 cut(s) 154, 732
HinP1I GCGC 2 cut(s) 154, 732
HpaII CCGG 1 cut(s) 501
HphI GGTGA 4 cut(s) 119, 276, 923, 1070
Hpy166II GTNNAC 2 cut(s) 144, 1078
Hpy188III TCNNGA 4 cut(s) 169, 213, 531, 773
Hpy8I GTNNAC 2 cut(s) 144, 1078
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 6 cut(s) 66, 184, 508, 742, 775, 1078
HpyCH4III ACNGT 1 cut(s) 742
HpyCH4IV ACGT 4 cut(s) 87, 787, 922, 1211
HpyCH4V TGCA 5 cut(s) 278, 473, 679, 807, 940
HpyF10VI GCNNNNNNNGC 3 cut(s) 851, 860, 1118
HpyF3I CTNAG 6 cut(s) 95, 392, 702, 876, 1101, 1155
HpySE526I ACGT 4 cut(s) 87, 787, 922, 1211
Hsp92I GRCGYC 1 cut(s) 732
Hsp92II CATG 5 cut(s) 73, 291, 428, 446, 1121
HspAI GCGC 2 cut(s) 154, 732
KasI GGCGCC 1 cut(s) 731
Kzo9I GATC 7 cut(s) 312, 517, 558, 565, 622, 646, 1017
Lsp1109I GCAGC 5 cut(s) 485, 663, 809, 863, 1059
LweI GCATC 1 cut(s) 555
MaeI CTAG 3 cut(s) 669, 872, 1136
MaeII ACGT 4 cut(s) 87, 787, 922, 1211
MaeIII GTNAC 4 cut(s) 634, 682, 911, 1041
MalI GATC 7 cut(s) 314, 519, 560, 567, 624, 648, 1019
MboI GATC 7 cut(s) 312, 517, 558, 565, 622, 646, 1017
MboII GAAGA 7 cut(s) 21, 24, 39, 51, 122, 215, 1197
MflI RGATCY 3 cut(s) 312, 622, 646
MhlI GDGCHC 2 cut(s) 192, 220
MlsI TGGCCA 1 cut(s) 892
MluCI AATT 4 cut(s) 124, 354, 493, 721
MluNI TGGCCA 1 cut(s) 892
Mly113I GGCGCC 1 cut(s) 732
MlyI GAGTC 4 cut(s) 575, 908, 971, 1169
MmeI TCCRAC 1 cut(s) 990
Mox20I TGGCCA 1 cut(s) 892
MscI TGGCCA 1 cut(s) 892
MseI TTAA 3 cut(s) 552, 714, 816
MslI CAYNNNNRTG 1 cut(s) 289
Msp20I TGGCCA 1 cut(s) 892
MspA1I CMGCKG 1 cut(s) 701
MspCI CTTAAG 1 cut(s) 713
MspI CCGG 1 cut(s) 501
MspR9I CCNGG 2 cut(s) 502, 608
Mva1269I GAATGC 1 cut(s) 937
MvaI CCWGG 1 cut(s) 608
MwoI GCNNNNNNNGC 3 cut(s) 851, 860, 1118
NarI GGCGCC 1 cut(s) 732
NciI CCSGG 1 cut(s) 502
NcoI CCATGG 1 cut(s) 287
NdeII GATC 7 cut(s) 312, 517, 558, 565, 622, 646, 1017
NlaIII CATG 5 cut(s) 73, 291, 428, 446, 1121
NlaIV GGNNCC 3 cut(s) 624, 692, 733
NmuCI GTSAC 3 cut(s) 682, 911, 1041
NsbI TGCGCA 1 cut(s) 155
NspI RCATGY 1 cut(s) 1121
OliI CACNNNNGTG 1 cut(s) 289
PaeR7I CTCGAG 1 cut(s) 213
PctI GAATGC 1 cut(s) 937
PfeI GAWTC 7 cut(s) 165, 239, 281, 329, 527, 824, 1030
Pfl23II CGTACG 1 cut(s) 919
PkrI GCNGC 6 cut(s) 475, 490, 678, 799, 853, 1049
PleI GAGTC 4 cut(s) 575, 908, 970, 1169
PluTI GGCGCC 1 cut(s) 735
PpsI GAGTC 4 cut(s) 575, 908, 970, 1169
Ppu21I YACGTR 2 cut(s) 923, 1212
Psp6I CCWGG 1 cut(s) 606
PspGI CCWGG 1 cut(s) 606
PspLI CGTACG 1 cut(s) 919
PspN4I GGNNCC 3 cut(s) 624, 692, 733
PspPI GGNCC 2 cut(s) 507, 690
PsuI RGATCY 3 cut(s) 312, 622, 646
PvuII CAGCTG 1 cut(s) 701
RsaI GTAC 4 cut(s) 143, 384, 400, 921
RsaNI GTAC 4 cut(s) 142, 383, 399, 920
RseI CAYNNNNRTG 1 cut(s) 289
SaqAI TTAA 3 cut(s) 552, 714, 816
SatI GCNGC 6 cut(s) 474, 489, 677, 798, 852, 1048
Sau3AI GATC 7 cut(s) 312, 517, 558, 565, 622, 646, 1017
Sau96I GGNCC 2 cut(s) 507, 690
SchI GAGTC 4 cut(s) 575, 908, 971, 1169
ScrFI CCNGG 2 cut(s) 502, 608
SduI GDGCHC 2 cut(s) 192, 220
SfaNI GCATC 1 cut(s) 555
SfcI CTRYAG 1 cut(s) 642
SfoI GGCGCC 1 cut(s) 733
Sfr274I CTCGAG 1 cut(s) 213
SinI GGWCC 1 cut(s) 690
SlaI CTCGAG 1 cut(s) 213
SmiMI CAYNNNNRTG 1 cut(s) 289
SmlI CTYRAG 2 cut(s) 213, 713
SmoI CTYRAG 2 cut(s) 213, 713
SnaBI TACGTA 1 cut(s) 1212
Sse9I AATT 4 cut(s) 124, 354, 493, 721
SsiI CCGC 1 cut(s) 488
SspDI GGCGCC 1 cut(s) 731
SspMI CTAG 3 cut(s) 669, 872, 1136
StyD4I CCNGG 2 cut(s) 500, 606
StyI CCWWGG 2 cut(s) 287, 316
TaaI ACNGT 1 cut(s) 742
TaiI ACGT 4 cut(s) 90, 790, 925, 1214
TaqI TCGA 5 cut(s) 122, 214, 432, 532, 1173
TasI AATT 4 cut(s) 124, 354, 493, 721
TatI WGTACW 1 cut(s) 141
TauI GCSGC 1 cut(s) 491
TfiI GAWTC 7 cut(s) 165, 239, 281, 329, 527, 824, 1030
Tru1I TTAA 3 cut(s) 552, 714, 816
Tru9I TTAA 3 cut(s) 552, 714, 816
TseFI GTSAC 3 cut(s) 682, 911, 1041
TseI GCWGC 5 cut(s) 473, 676, 797, 851, 1047
Tsp45I GTSAC 3 cut(s) 682, 911, 1041
TspDTI ATGAA 5 cut(s) 166, 252, 441, 540, 627
TspGWI ACGGA 1 cut(s) 907
Vha464I CTTAAG 1 cut(s) 713
VpaK11BI GGWCC 1 cut(s) 690
XapI RAATTY 2 cut(s) 124, 493
XceI RCATGY 1 cut(s) 1121
XcmI CCANNNNNNNNNTGG 1 cut(s) 604
XhoI CTCGAG 1 cut(s) 213
XspI CTAG 3 cut(s) 669, 872, 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.