RLG00000035241

membrane protein At1g06890-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013328
Physical Location & Seq
Forward (+)
63413408 .. 63415276
1869 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035241

Sequence Viewer

Length: 996 bp
ATGACTGGCTTCCAATTGGGCGTTATTGGTGCACTGTTCCTTTCAGTGGCATCATCTGTGTCCATTGTTATATGCAACAAAGCCTTGATGAGCAACCTTGGCTTCCCTTTTGCAACAACACTTACCAGTTGGCATCTCATGGTAACTTTCTGCACACTTCATGTGGCAAATCGTCTCAATTTCTTCGAGAGCAAGTCAATTGACATGAAGACTGTCATGCTCTTTGGCATTCTAAATGGTGTTTCCATTGGACTACTCAATTTGAGTCTGGGATTCAATTCTGTTGGGTTCTACCAGATGACCAAGCTTGCAATCATACCATTTACTGTGTTAGTGGAGACTCTTTTCCTAAAAAAGCAATTCAGCCAGCAAATAAAGTTTTCTCTCTTCCTATTACTTGTTGGAGTTGGCATTGCCTCTGTGACAGACCTCCAGCTCAATTTTGTTGGAACCATTCTCTCTCTTCTAGCCATCATAACAACCTGTGTTGGACAAATTCTAACAAACACAATACAGAAGAGGCTCAATGTTTCTTCTACTCAGCTACTCTACCAGTCAGCACCTTTTCAAGCAGCTATTCTTTTCGTCTCCGGACCTGTGGTGGATCAGTTCCTCACCAAGCAAAGCGTGTTTTCCCACAAATACTCCCCCATAGTCTTGGTATTCATCATACTCTCATGCCTAATAGCTGTGTCTGTGAACTTTAGCACCTTTTTGGTGATTGGCAAGACATCACCAGTGACATATCAAGTGCTAGGGCACCTCAAGACATGCCTTGTTCTAGGGTTCGGCTATACATTACTACATGACCCTTTCACTGAGAGGAACATAATCGGAATTCTGGTAGCCATTTTTGGGATGGGATTGTATTCATATTTCTGTGCACAAGAGAACAAAAAGAAACAAGCAGCTGACGTTAAGGATGGTGCTCCACTTTTGAGTCAGGATAAAGATAGCCATGAGGTTAAGAAATTAACCAAGAACTCTCTTGTTTGA

Protein Analysis

332

Amino Acids

36.19

Weight (kDa)

9.34

Isoelectric Point (pI)

33.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 18 - 291 4.4e-20 Triose-phosphate Transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013328)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28315 AT2G28315
fragaria_vesca FvH4_3g31910 FvH4_3g31910 FvH4_3g31910
malus_domestica MD03G1120000.v1.1 MD11G1138100.v1.1
prunus_persica Prupe.6G103500_v2.0.a1
pyrus_communis pycom11g11410
rosa_chinensis RchiOBHm_Chr5g0058681
rosa_laevigata RLG00000035241
rosa_roxburghii Rroxscaffold_1G00021730
rosa_rugosa Rorug05G0317400
rosa_samantha Rh5AG383100 Rh5BG395000 Rh5CG418800 Rh5DG410300
rosa_wichuraiana Rw5G036100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 759
AccIII TCCGGA 1 cut(s) 590
AclWI GGATC 1 cut(s) 612
AcsI RAATTY 2 cut(s) 495, 837
AfiI CCNNNNNNNGG 2 cut(s) 46, 855
AgsI TTSAA 2 cut(s) 277, 569
AluBI AGCT 6 cut(s) 307, 436, 544, 575, 689, 911
AluI AGCT 6 cut(s) 307, 436, 544, 575, 689, 911
Alw21I GWGCWC 3 cut(s) 34, 886, 931
Alw26I GTCTC 3 cut(s) 179, 332, 592
Alw44I GTGCAC 2 cut(s) 30, 882
AlwI GGATC 1 cut(s) 612
Aor13HI TCCGGA 1 cut(s) 590
ApaLI GTGCAC 2 cut(s) 30, 882
ApeKI GCWGC 2 cut(s) 572, 908
ApoI RAATTY 2 cut(s) 495, 837
AspS9I GGNCC 1 cut(s) 593
AsuHPI GGTGA 3 cut(s) 607, 726, 730
AvaII GGWCC 1 cut(s) 593
BaeGI GKGCMC 3 cut(s) 34, 762, 886
BanI GGYRCC 1 cut(s) 759
BbsI GAAGAC 1 cut(s) 215
Bbv12I GWGCWC 3 cut(s) 34, 886, 931
BbvI GCAGC 2 cut(s) 584, 920
BccI CCATC 3 cut(s) 479, 853, 917
BcoDI GTCTC 3 cut(s) 179, 332, 592
BfaI CTAG 3 cut(s) 467, 755, 782
BisI GCNGC 2 cut(s) 573, 909
BlsI GCNGC 2 cut(s) 574, 910
Bme18I GGWCC 1 cut(s) 593
BmgT120I GGNCC 1 cut(s) 593
BmiI GGNNCC 2 cut(s) 451, 761
BmsI GCATC 2 cut(s) 59, 142
BpiI GAAGAC 1 cut(s) 215
BpmI CTGGAG 1 cut(s) 416
BpuEI CTTGAG 1 cut(s) 749
BsaJI CCNNGG 1 cut(s) 97
BsaWI WCCGGW 1 cut(s) 590
BsaXI ACNNNNNCTCC 2 cut(s) 629, 659
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 855
Bse1I ACTGG 4 cut(s) 10, 126, 553, 737
Bse3DI GCAATG 1 cut(s) 411
BseAI TCCGGA 1 cut(s) 590
BseDI CCNNGG 1 cut(s) 97
BseGI GGATG 2 cut(s) 864, 928
BseLI CCNNNNNNNGG 2 cut(s) 46, 855
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 2 cut(s) 554, 810
BseNI ACTGG 4 cut(s) 10, 126, 553, 737
BseSI GKGCMC 3 cut(s) 34, 762, 886
BseXI GCAGC 2 cut(s) 584, 920
BsgI GTGCAG 1 cut(s) 136
BshNI GGYRCC 1 cut(s) 759
BsiHKAI GWGCWC 3 cut(s) 34, 886, 931
BsiSI CCGG 1 cut(s) 591
BslI CCNNNNNNNGG 2 cut(s) 46, 855
BsmAI GTCTC 3 cut(s) 179, 332, 592
BsmBI CGTCTC 2 cut(s) 179, 592
BsmI GAATGC 1 cut(s) 228
Bsp1286I GDGCHC 4 cut(s) 34, 762, 886, 931
Bsp13I TCCGGA 1 cut(s) 590
Bsp143I GATC 1 cut(s) 604
BspCNI CTCAG 2 cut(s) 553, 811
BspEI TCCGGA 1 cut(s) 590
BspLI GGNNCC 2 cut(s) 451, 761
BspPI GGATC 1 cut(s) 612
BspT107I GGYRCC 1 cut(s) 759
BsrDI GCAATG 1 cut(s) 411
BsrI ACTGG 4 cut(s) 10, 126, 553, 737
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 1 cut(s) 604
BssT1I CCWWGG 1 cut(s) 97
Bst4CI ACNGT 3 cut(s) 36, 214, 328
Bst6I CTCTTC 3 cut(s) 392, 468, 512
BstC8I GCNNGC 2 cut(s) 309, 368
BstDEI CTNAG 2 cut(s) 540, 819
BstF5I GGATG 2 cut(s) 864, 928
BstKTI GATC 1 cut(s) 607
BstMAI GTCTC 3 cut(s) 179, 332, 592
BstMBI GATC 1 cut(s) 604
BstMWI GCNNNNNNNGC 1 cut(s) 99
BstNSI RCATGY 1 cut(s) 774
BstSLI GKGCMC 3 cut(s) 34, 762, 886
BstV1I GCAGC 2 cut(s) 584, 920
BstV2I GAAGAC 1 cut(s) 215
BstXI CCANNNNNNTGG 1 cut(s) 658
BtsCI GGATG 2 cut(s) 864, 928
BtsIMutI CAGTG 4 cut(s) 32, 51, 744, 816
Cac8I GCNNGC 2 cut(s) 309, 368
Cfr13I GGNCC 1 cut(s) 593
CviAII CATG 8 cut(s) 139, 161, 205, 217, 678, 771, 806, 959
DdeI CTNAG 2 cut(s) 540, 819
DpnI GATC 1 cut(s) 606
DpnII GATC 1 cut(s) 604
Eam1104I CTCTTC 3 cut(s) 392, 468, 512
EarI CTCTTC 3 cut(s) 392, 468, 512
Eco130I CCWWGG 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 593
EcoRI GAATTC 1 cut(s) 837
EcoT14I CCWWGG 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 97
Esp3I CGTCTC 2 cut(s) 179, 592
FaeI CATG 8 cut(s) 142, 164, 208, 220, 681, 774, 809, 962
FatI CATG 8 cut(s) 138, 160, 204, 216, 677, 770, 805, 958
Fnu4HI GCNGC 2 cut(s) 573, 909
FokI GGATG 2 cut(s) 871, 935
Fsp4HI GCNGC 2 cut(s) 573, 909
FspBI CTAG 3 cut(s) 467, 755, 782
GluI GCNGC 2 cut(s) 573, 909
GsuI CTGGAG 1 cut(s) 416
HapII CCGG 1 cut(s) 591
Hin1II CATG 8 cut(s) 142, 164, 208, 220, 681, 774, 809, 962
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 4 cut(s) 265, 273, 340, 940
HpaII CCGG 1 cut(s) 591
HphI GGTGA 3 cut(s) 607, 726, 730
Hpy166II GTNNAC 3 cut(s) 32, 700, 884
Hpy188I TCNGA 1 cut(s) 836
Hpy188III TCNNGA 4 cut(s) 187, 591, 766, 944
Hpy8I GTNNAC 3 cut(s) 32, 700, 884
HpyCH4III ACNGT 3 cut(s) 36, 214, 328
HpyCH4IV ACGT 1 cut(s) 915
HpyCH4V TGCA 6 cut(s) 32, 75, 113, 153, 311, 884
HpyF10VI GCNNNNNNNGC 1 cut(s) 99
HpyF3I CTNAG 2 cut(s) 540, 819
HpySE526I ACGT 1 cut(s) 915
Hsp92II CATG 8 cut(s) 142, 164, 208, 220, 681, 774, 809, 962
Kpn2I TCCGGA 1 cut(s) 590
Kzo9I GATC 1 cut(s) 604
LmnI GCTCC 1 cut(s) 934
Lsp1109I GCAGC 2 cut(s) 584, 920
LweI GCATC 2 cut(s) 59, 142
MaeI CTAG 3 cut(s) 467, 755, 782
MaeII ACGT 1 cut(s) 915
MaeIII GTNAC 3 cut(s) 142, 421, 739
MalI GATC 1 cut(s) 606
MboI GATC 1 cut(s) 604
MboII GAAGA 6 cut(s) 175, 220, 379, 455, 525, 529
MfeI CAATTG 2 cut(s) 14, 198
MhlI GDGCHC 4 cut(s) 34, 762, 886, 931
MlyI GAGTC 3 cut(s) 274, 334, 949
MmeI TCCRAC 3 cut(s) 382, 427, 469
MnlI CCTC 7 cut(s) 427, 440, 513, 623, 773, 816, 955
MroI TCCGGA 1 cut(s) 590
MseI TTAA 3 cut(s) 918, 966, 974
MspA1I CMGCKG 1 cut(s) 911
MspI CCGG 1 cut(s) 591
MunI CAATTG 2 cut(s) 14, 198
Mva1269I GAATGC 1 cut(s) 228
MwoI GCNNNNNNNGC 1 cut(s) 99
NdeII GATC 1 cut(s) 604
NlaIII CATG 8 cut(s) 142, 164, 208, 220, 681, 774, 809, 962
NlaIV GGNNCC 2 cut(s) 451, 761
NmuCI GTSAC 2 cut(s) 421, 739
NspI RCATGY 1 cut(s) 774
PctI GAATGC 1 cut(s) 228
PfeI GAWTC 1 cut(s) 273
PkrI GCNGC 2 cut(s) 574, 910
PleI GAGTC 3 cut(s) 273, 334, 948
PpsI GAGTC 3 cut(s) 273, 334, 948
PspN4I GGNNCC 2 cut(s) 451, 761
PspPI GGNCC 1 cut(s) 593
PvuII CAGCTG 1 cut(s) 911
SaqAI TTAA 3 cut(s) 918, 966, 974
SatI GCNGC 2 cut(s) 573, 909
Sau3AI GATC 1 cut(s) 604
Sau96I GGNCC 1 cut(s) 593
SchI GAGTC 3 cut(s) 274, 334, 949
SduI GDGCHC 4 cut(s) 34, 762, 886, 931
SfaNI GCATC 2 cut(s) 59, 142
SinI GGWCC 1 cut(s) 593
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
SspMI CTAG 3 cut(s) 467, 755, 782
StyI CCWWGG 1 cut(s) 97
TaaI ACNGT 3 cut(s) 36, 214, 328
TaiI ACGT 1 cut(s) 918
TaqI TCGA 1 cut(s) 186
TfiI GAWTC 1 cut(s) 273
Tru1I TTAA 3 cut(s) 918, 966, 974
Tru9I TTAA 3 cut(s) 918, 966, 974
TscAI CASTG 4 cut(s) 39, 51, 744, 823
TseFI GTSAC 2 cut(s) 421, 739
TseI GCWGC 2 cut(s) 572, 908
Tsp45I GTSAC 2 cut(s) 421, 739
TspDTI ATGAA 4 cut(s) 149, 221, 655, 861
TspRI CASTG 4 cut(s) 39, 51, 744, 823
VneI GTGCAC 2 cut(s) 30, 882
VpaK11BI GGWCC 1 cut(s) 593
XapI RAATTY 2 cut(s) 495, 837
XceI RCATGY 1 cut(s) 774
XcmI CCANNNNNNNNNTGG 1 cut(s) 856
XspI CTAG 3 cut(s) 467, 755, 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.