RLG00000035743

methyl-CpG-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014187
Physical Location & Seq
Forward (+)
71132081 .. 71135059
2979 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035743

Sequence Viewer

Length: 705 bp
ATGTCAGCCCCCGAAGCTTCCGGTAAAGATCTGAACCCGAACCCGAATCCGACTCCGACTCCGAGTCCACCTCATCAACACCACCTGAATCCTCCACCTGACGGAACCTTCGACGGCGCCGATCCCCTCCTCCAATCGGGGGCCTTCATCGACCCCACCACCTCAGATATGGAAGAAACCGGGAAGGCCCAACCGAACGGTGTGGACTCAGTGACGCCGGGCACGAGTAATGTCACTGCAGACGGGTCGGCGGGCCGGGCTAAGGCGAAAGGGTCGGATCCTCAGGCTATGGATTGGTTGCCGGCCGGTTGGAGAGTGGAGTATAAAGTGCGGAGCTCCGGTGCTACAGCTGGAAGTACAGATAGGTATTACCACGATCCAGTCTCAGGTCGTCGATTTCGGTCAAAGAAAGAAGTCCTTCACTTTCTGGAAACAGGAACGCTCAAGAAGAACGCTAGTTCTGACAAAACGTCCGTGGAAGGTTCTGCGAGCAAAAAGCAAAGGAAGTCTAGTACAAAGCCCAGACCTGCTGTGAACTTTGATTTTTTTGATGTGCCTGCGAAGGTGGAATGGGTTCTCACAGACTCCTCTCAGTGGACCTGGACACCCTTTATCGGTGACCAAAAGGTGCCCGAGTCCACTGCCAAAGAATGGGCTACTGCATTCACAGTTTTCACATCCAGCAATAGTGGCCACACTCAATGA

Protein Analysis

235

Amino Acids

25.19

Weight (kDa)

6.6

Isoelectric Point (pI)

34.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBD PF01429 98 - 148 1.4e-11 Methyl-CpG binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46580 AT5G59380
fragaria_vesca FvH4_3g36180
malus_domestica MD03G1092700.v1.1 MD11G1107500.v1.1
prunus_persica Prupe.6G076700_v2.0.a1
pyrus_communis pycom03g07410
rosa_chinensis RchiOBHm_Chr5g0064931
rosa_laevigata RLG00000035743
rosa_multiflora Rmu_sc0003352.1_g000016
rosa_roxburghii Rroxscaffold_1G00016390
rosa_rugosa Rorug05G0366700
rosa_samantha Rh5AG425200 Rh5BG440000 Rh5CG462700 Rh5DG453200
rosa_wichuraiana Rw5G039950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 535
AccB1I GGYRCC 2 cut(s) 116, 628
AccB7I CCANNNNNTGG 1 cut(s) 651
AciI CCGC 2 cut(s) 251, 331
AclWI GGATC 4 cut(s) 116, 272, 285, 371
AcoI YGGCCR 2 cut(s) 303, 691
AcyI GRCGYC 2 cut(s) 117, 215
AfaI GTAC 2 cut(s) 358, 514
AfiI CCNNNNNNNGG 8 cut(s) 101, 136, 139, 262, 386, 594, 614, 651
AhdI GACNNNNNGTC 2 cut(s) 63, 469
AjnI CCWGG 1 cut(s) 599
AluBI AGCT 3 cut(s) 17, 336, 350
AluI AGCT 3 cut(s) 17, 336, 350
Alw21I GWGCWC 1 cut(s) 338
Alw26I GTCTC 1 cut(s) 388
AlwI GGATC 4 cut(s) 116, 272, 285, 371
Ama87I CYCGRG 1 cut(s) 632
AoxI GGCC 5 cut(s) 141, 186, 253, 303, 691
ArsI GACNNNNNNTTYG 2 cut(s) 92, 124
Asp700I GAANNNNTTC 2 cut(s) 417, 573
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 4 cut(s) 141, 187, 253, 597
AsuC2I CCSGG 3 cut(s) 181, 219, 257
AsuHPI GGTGA 1 cut(s) 629
AvaI CYCGRG 1 cut(s) 632
AvaII GGWCC 1 cut(s) 597
AxyI CCTNAGG 1 cut(s) 282
BaeGI GKGCMC 2 cut(s) 224, 633
BalI TGGCCA 1 cut(s) 693
BamHI GGATCC 1 cut(s) 277
BanI GGYRCC 2 cut(s) 116, 628
BanII GRGCYC 1 cut(s) 338
BauI CACGAG 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 338
BceAI ACGGC 1 cut(s) 130
BcgI CGANNNNNNTGC 4 cut(s) 228, 262, 623, 657
BciT130I CCWGG 1 cut(s) 601
BcnI CCSGG 3 cut(s) 181, 219, 257
BcoDI GTCTC 1 cut(s) 388
BfaI CTAG 2 cut(s) 456, 510
BfmI CTRYAG 2 cut(s) 237, 345
BfoI RGCGCY 1 cut(s) 120
BfuAI ACCTGC 1 cut(s) 535
BglII AGATCT 1 cut(s) 28
Bme1390I CCNGG 4 cut(s) 181, 219, 257, 601
Bme18I GGWCC 1 cut(s) 597
BmeRI GACNNNNNGTC 2 cut(s) 63, 469
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 4 cut(s) 141, 187, 253, 597
BmiI GGNNCC 5 cut(s) 106, 118, 142, 279, 630
BmrFI CCNGG 4 cut(s) 181, 219, 257, 601
BplI GAGNNNNNCTC 2 cut(s) 55, 87
Bpu10I CCTNAGC 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 428
BpuMI CCSGG 3 cut(s) 181, 219, 257
BsaHI GRCGYC 2 cut(s) 117, 215
BsaJI CCNNGG 1 cut(s) 474
BsaWI WCCGGW 2 cut(s) 20, 338
BsaXI ACNNNNNCTCC 4 cut(s) 43, 73, 325, 355
Bsc4I CCNNNNNNNGG 8 cut(s) 101, 136, 139, 262, 386, 594, 614, 651
Bse118I RCCGGY 2 cut(s) 301, 305
Bse1I ACTGG 1 cut(s) 380
Bse21I CCTNAGG 1 cut(s) 282
BseBI CCWGG 1 cut(s) 601
BseDI CCNNGG 1 cut(s) 474
BseGI GGATG 1 cut(s) 677
BseLI CCNNNNNNNGG 8 cut(s) 101, 136, 139, 262, 386, 594, 614, 651
BseMII CTCAG 5 cut(s) 177, 222, 296, 399, 605
BseNI ACTGG 1 cut(s) 380
BseRI GAGGAG 2 cut(s) 119, 577
BseSI GKGCMC 2 cut(s) 224, 633
BseX3I CGGCCG 1 cut(s) 303
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 5 cut(s) 143, 188, 255, 305, 693
BshNI GGYRCC 2 cut(s) 116, 628
BsiEI CGRYCG 1 cut(s) 306
BsiHKAI GWGCWC 1 cut(s) 338
BsiHKCI CYCGRG 1 cut(s) 632
BsiSI CCGG 7 cut(s) 21, 180, 218, 256, 302, 306, 339
BslI CCNNNNNNNGG 8 cut(s) 101, 136, 139, 262, 386, 594, 614, 651
BsmAI GTCTC 1 cut(s) 388
BsmI GAATGC 1 cut(s) 662
BsnI GGCC 5 cut(s) 143, 188, 255, 305, 693
BsoBI CYCGRG 1 cut(s) 632
Bsp1286I GDGCHC 3 cut(s) 224, 338, 633
Bsp143I GATC 4 cut(s) 28, 121, 277, 376
BspACI CCGC 2 cut(s) 251, 331
BspANI GGCC 5 cut(s) 143, 188, 255, 305, 693
BspCNI CTCAG 5 cut(s) 176, 221, 295, 398, 604
BspLI GGNNCC 5 cut(s) 106, 118, 142, 279, 630
BspMAI CTGCAG 1 cut(s) 241
BspMI ACCTGC 1 cut(s) 535
BspPI GGATC 4 cut(s) 116, 272, 285, 371
BspT107I GGYRCC 2 cut(s) 116, 628
BsrFI RCCGGY 2 cut(s) 301, 305
BsrI ACTGG 1 cut(s) 380
BssAI RCCGGY 2 cut(s) 301, 305
BssECI CCNNGG 1 cut(s) 474
BssMI GATC 4 cut(s) 28, 121, 277, 376
BssNI GRCGYC 2 cut(s) 117, 215
BssSI CACGAG 1 cut(s) 223
Bst2BI CACGAG 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 601
Bst4CI ACNGT 2 cut(s) 200, 670
BstACI GRCGYC 2 cut(s) 117, 215
BstC8I GCNNGC 4 cut(s) 253, 303, 490, 558
BstDEI CTNAG 6 cut(s) 163, 208, 261, 282, 385, 591
BstDSI CCRYGG 1 cut(s) 474
BstEII GGTNACC 1 cut(s) 617
BstF5I GGATG 1 cut(s) 677
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 4 cut(s) 31, 124, 280, 379
BstMAI GTCTC 1 cut(s) 388
BstMBI GATC 4 cut(s) 28, 121, 277, 376
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 3 cut(s) 14, 257, 690
BstNI CCWGG 1 cut(s) 601
BstPI GGTNACC 1 cut(s) 617
BstSCI CCNGG 4 cut(s) 179, 217, 255, 599
BstSFI CTRYAG 2 cut(s) 237, 345
BstSLI GKGCMC 2 cut(s) 224, 633
BstX2I RGATCY 2 cut(s) 28, 277
BstYI RGATCY 2 cut(s) 28, 277
BstZI CGGCCG 1 cut(s) 303
Bsu36I CCTNAGG 1 cut(s) 282
BsuRI GGCC 5 cut(s) 143, 188, 255, 305, 693
BtgI CCRYGG 1 cut(s) 474
BtsCI GGATG 1 cut(s) 677
BtsI GCAGTG 2 cut(s) 234, 639
BtsIMutI CAGTG 4 cut(s) 216, 234, 599, 639
BveI ACCTGC 1 cut(s) 535
Cac8I GCNNGC 4 cut(s) 253, 303, 490, 558
CfoI GCGC 1 cut(s) 119
Cfr10I RCCGGY 2 cut(s) 301, 305
Cfr13I GGNCC 4 cut(s) 141, 187, 253, 597
CseI GACGC 1 cut(s) 223
Csp6I GTAC 2 cut(s) 357, 513
CviQI GTAC 2 cut(s) 357, 513
DdeI CTNAG 6 cut(s) 163, 208, 261, 282, 385, 591
DinI GGCGCC 1 cut(s) 118
DpnI GATC 4 cut(s) 30, 123, 279, 378
DpnII GATC 4 cut(s) 28, 121, 277, 376
DriI GACNNNNNGTC 2 cut(s) 63, 469
EaeI YGGCCR 2 cut(s) 303, 691
EagI CGGCCG 1 cut(s) 303
Eam1105I GACNNNNNGTC 2 cut(s) 63, 469
Ecl136II GAGCTC 1 cut(s) 336
EclXI CGGCCG 1 cut(s) 303
Eco24I GRGCYC 1 cut(s) 338
Eco47I GGWCC 1 cut(s) 597
Eco52I CGGCCG 1 cut(s) 303
Eco53kI GAGCTC 1 cut(s) 336
Eco81I CCTNAGG 1 cut(s) 282
Eco88I CYCGRG 1 cut(s) 632
Eco91I GGTNACC 1 cut(s) 617
EcoICRI GAGCTC 1 cut(s) 336
EcoO109I RGGNCCY 1 cut(s) 141
EcoO65I GGTNACC 1 cut(s) 617
EcoRII CCWGG 1 cut(s) 599
EcoT38I GRGCYC 1 cut(s) 338
EgeI GGCGCC 1 cut(s) 118
EheI GGCGCC 1 cut(s) 118
FaiI YATR 3 cut(s) 170, 290, 324
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FauI CCCGC 1 cut(s) 244
FokI GGATG 1 cut(s) 664
FriOI GRGCYC 1 cut(s) 338
FspBI CTAG 2 cut(s) 456, 510
GlaI GCGC 1 cut(s) 118
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 5 cut(s) 143, 188, 255, 305, 693
HapII CCGG 7 cut(s) 21, 180, 218, 256, 302, 306, 339
HgaI GACGC 1 cut(s) 223
HhaI GCGC 1 cut(s) 119
Hin1I GRCGYC 2 cut(s) 117, 215
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 8 cut(s) 46, 52, 58, 64, 88, 206, 584, 635
HpaII CCGG 7 cut(s) 21, 180, 218, 256, 302, 306, 339
HphI GGTGA 1 cut(s) 629
Hpy166II GTNNAC 5 cut(s) 68, 205, 535, 597, 639
Hpy188I TCNGA 7 cut(s) 33, 51, 57, 63, 166, 277, 463
Hpy188III TCNNGA 2 cut(s) 428, 445
Hpy8I GTNNAC 5 cut(s) 68, 205, 535, 597, 639
Hpy99I CGWCG 2 cut(s) 116, 396
HpyAV CCTTC 6 cut(s) 118, 154, 178, 428, 473, 556
HpyCH4III ACNGT 2 cut(s) 200, 670
HpyCH4IV ACGT 1 cut(s) 470
HpyCH4V TGCA 2 cut(s) 239, 662
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 257, 690
HpyF3I CTNAG 6 cut(s) 163, 208, 261, 282, 385, 591
HpySE526I ACGT 1 cut(s) 470
Hsp92I GRCGYC 2 cut(s) 117, 215
HspAI GCGC 1 cut(s) 117
KasI GGCGCC 1 cut(s) 116
KroI GCCGGC 1 cut(s) 301
KroNI GCCGGC 1 cut(s) 303
Kzo9I GATC 4 cut(s) 28, 121, 277, 376
LmnI GCTCC 2 cut(s) 333, 341
MaeI CTAG 2 cut(s) 456, 510
MaeII ACGT 1 cut(s) 470
MaeIII GTNAC 3 cut(s) 211, 232, 617
MalI GATC 4 cut(s) 30, 123, 279, 378
MboI GATC 4 cut(s) 28, 121, 277, 376
MboII GAAGA 2 cut(s) 185, 460
MflI RGATCY 2 cut(s) 28, 277
MhlI GDGCHC 3 cut(s) 224, 338, 633
MlsI TGGCCA 1 cut(s) 693
MluNI TGGCCA 1 cut(s) 693
Mly113I GGCGCC 1 cut(s) 117
MlyI GAGTC 6 cut(s) 46, 52, 73, 200, 578, 644
MmeI TCCRAC 4 cut(s) 74, 80, 255, 290
MnlI CCTC 7 cut(s) 81, 102, 137, 140, 172, 291, 598
Mox20I TGGCCA 1 cut(s) 693
MroNI GCCGGC 1 cut(s) 301
MroXI GAANNNNTTC 2 cut(s) 417, 573
MscI TGGCCA 1 cut(s) 693
Msp20I TGGCCA 1 cut(s) 693
MspA1I CMGCKG 1 cut(s) 350
MspI CCGG 7 cut(s) 21, 180, 218, 256, 302, 306, 339
MspR9I CCNGG 4 cut(s) 181, 219, 257, 601
Mva1269I GAATGC 1 cut(s) 662
MvaI CCWGG 1 cut(s) 601
MwoI GCNNNNNNNGC 3 cut(s) 14, 257, 690
NaeI GCCGGC 1 cut(s) 303
NarI GGCGCC 1 cut(s) 117
NciI CCSGG 3 cut(s) 181, 219, 257
NdeII GATC 4 cut(s) 28, 121, 277, 376
NgoMIV GCCGGC 1 cut(s) 301
NlaIV GGNNCC 5 cut(s) 106, 118, 142, 279, 630
NmuCI GTSAC 3 cut(s) 211, 232, 617
PcsI WCGNNNNNNNCGW 3 cut(s) 108, 117, 221
PctI GAATGC 1 cut(s) 662
PdiI GCCGGC 1 cut(s) 303
PdmI GAANNNNTTC 2 cut(s) 417, 573
PfeI GAWTC 2 cut(s) 46, 88
PflMI CCANNNNNTGG 1 cut(s) 651
PleI GAGTC 6 cut(s) 46, 52, 72, 200, 578, 643
PluTI GGCGCC 1 cut(s) 120
PpsI GAGTC 6 cut(s) 46, 52, 72, 200, 578, 643
Psp124BI GAGCTC 1 cut(s) 338
Psp6I CCWGG 1 cut(s) 599
PspEI GGTNACC 1 cut(s) 617
PspGI CCWGG 1 cut(s) 599
PspN4I GGNNCC 5 cut(s) 106, 118, 142, 279, 630
PspPI GGNCC 4 cut(s) 141, 187, 253, 597
PstI CTGCAG 1 cut(s) 241
PsuI RGATCY 2 cut(s) 28, 277
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 2 cut(s) 358, 514
RsaNI GTAC 2 cut(s) 357, 513
SacI GAGCTC 1 cut(s) 338
Sau3AI GATC 4 cut(s) 28, 121, 277, 376
Sau96I GGNCC 4 cut(s) 141, 187, 253, 597
SchI GAGTC 6 cut(s) 46, 52, 73, 200, 578, 644
ScrFI CCNGG 4 cut(s) 181, 219, 257, 601
SduI GDGCHC 3 cut(s) 224, 338, 633
SfcI CTRYAG 2 cut(s) 237, 345
SfoI GGCGCC 1 cut(s) 118
SinI GGWCC 1 cut(s) 597
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
SsiI CCGC 2 cut(s) 251, 331
SspDI GGCGCC 1 cut(s) 116
SspMI CTAG 2 cut(s) 456, 510
SstI GAGCTC 1 cut(s) 338
StyD4I CCNGG 4 cut(s) 179, 217, 255, 599
TaaI ACNGT 2 cut(s) 200, 670
TaiI ACGT 1 cut(s) 473
TaqI TCGA 3 cut(s) 111, 150, 394
TaqII GACCGA 1 cut(s) 390
TatI WGTACW 2 cut(s) 356, 512
TfiI GAWTC 2 cut(s) 46, 88
TscAI CASTG 4 cut(s) 216, 241, 599, 646
TseFI GTSAC 3 cut(s) 211, 232, 617
Tsp45I GTSAC 3 cut(s) 211, 232, 617
TspDTI ATGAA 1 cut(s) 136
TspGWI ACGGA 2 cut(s) 117, 463
TspRI CASTG 4 cut(s) 216, 241, 599, 646
Van91I CCANNNNNTGG 1 cut(s) 651
VpaK11BI GGWCC 1 cut(s) 597
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XmnI GAANNNNTTC 2 cut(s) 417, 573
XspI CTAG 2 cut(s) 456, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.