Rorug05G0366700

methyl-CpG-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014187
Physical Location & Seq
Reverse (-)
48881379 .. 48884400
3022 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0366700.1

Sequence Viewer

Length: 1128 bp
ATGAACGAACTGGGTGGTCCTGCTCTTACTCAAATTTATGTCATGGGTATTGGTGAAGCAGAGGCATTTTCTGAAAGGTTGAAAAGAGAACTTCTGGCTCTGGAAGCAGCAAATGTGCATGCCATTTTGGAAAGTGAACCTTTGATTGATGAGCTGATTGCTGAGGCTCAACTCTGTGGTTTGGAACTGTATGAGAAAATTATCTTGATTTGCACAACTGGTCATAAGCACTCCGGTTCAATCGAGCCTCTAAAAGATAATGGGTTCCCTTATGCACCGAAGTTATCATCGGCTATGCTTTCGTTATTTATTATCCTTGTCCAATCAGAGCTTGAGCATGAACAACTTTCCACTTTGAAACTTCTGCACCTTCTCTTGAAGTGGAAGTATGGAAACGAAAATGTAGACAGAAGTGGAGGTGCCATGAGCGAGGAGCTTTTGTTCATGTTTCCTGTCGTTGGTCTACTGCCATCTCCTTCTAAACATGTCAAAGGAGCGGCAACAGATCTGCTTGTCATGCTAGAAAGACTTTTAGTAAGAGTTTTGGTAGCTCCAAAGGATAAACCAGCAAAAGAAGCAGGGCATCCCTCTCTAAGCACACCTGGATCTATTGTGTTTAGAATCTTACAGCATCTCTGGTTTCAGGATTCTTCTTCATTGTCCAGTTTGTTTTTAAATTTTTCTTCTATCAGTAAAACTAATGGCGAGGAGATTTATGATGTACCAAGATCGTGGGCTTCTCATTTACAAGAGTATACTCTATTGATTGTTGACAAACGAAAGTCCTCCCTACCTCTCTCGCAGACTCAAGAAATATACATAACTGAGATGCCCTCGTTACTCTCTGCCATTGCTGGTCTTTTAGTGATGAATAAGAGGATTGGGCAAGTACTCATGCATTGGATACTTGATTCGGAGCCCAGCTCAAGTCTCGGTTCTCTATTCCTTTTTGCTGATACCAATTTACTTTATGCTGATCTCTACTTGTGCAGAGAGCATAATCTGCCAATATTTGATTGTAATCCCAGCTTGATCAACTTCCAAACAGAGAGGATAAGCACTTTATCTTCCTTCATCAACAGAATCTTCCTTGTAGTTTGTCCGCCAAACAAAGATAGTACGAGCTGA

Protein Analysis

375

Amino Acids

41.7

Weight (kDa)

5.42

Isoelectric Point (pI)

48.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3730 PF12530 90 - 146 1.2e-08 Focadhesin/RST1, DUF3730
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46580 AT5G59380
fragaria_vesca FvH4_3g36180
malus_domestica MD03G1092700.v1.1 MD11G1107500.v1.1
prunus_persica Prupe.6G076700_v2.0.a1
pyrus_communis pycom03g07410
rosa_chinensis RchiOBHm_Chr5g0064931
rosa_laevigata RLG00000035743
rosa_multiflora Rmu_sc0003352.1_g000016
rosa_roxburghii Rroxscaffold_1G00016390
rosa_rugosa Rorug05G0366700
rosa_samantha Rh5AG425200 Rh5BG440000 Rh5CG462700 Rh5DG453200
rosa_wichuraiana Rw5G039950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 419
AccBSI CCGCTC 1 cut(s) 497
AccI GTMKAC 3 cut(s) 405, 463, 755
AciI CCGC 2 cut(s) 497, 1103
AclWI GGATC 1 cut(s) 613
AcsI RAATTY 2 cut(s) 33, 676
AfaI GTAC 3 cut(s) 723, 891, 1120
AfiI CCNNNNNNNGG 1 cut(s) 458
AflIII ACRYGT 1 cut(s) 484
AgsI TTSAA 4 cut(s) 82, 240, 358, 379
AjnI CCWGG 1 cut(s) 601
AloI GAACNNNNNNTCC 2 cut(s) 425, 457
AluBI AGCT 7 cut(s) 154, 331, 436, 551, 924, 1029, 1125
AluI AGCT 7 cut(s) 154, 331, 436, 551, 924, 1029, 1125
Alw26I GTCTC 1 cut(s) 935
AlwI GGATC 1 cut(s) 613
ApeKI GCWGC 1 cut(s) 107
ApoI RAATTY 2 cut(s) 33, 676
AspS9I GGNCC 1 cut(s) 17
AsuHPI GGTGA 1 cut(s) 65
AvaII GGWCC 1 cut(s) 17
BanI GGYRCC 1 cut(s) 419
BanII GRGCYC 1 cut(s) 921
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 119
BccI CCATC 1 cut(s) 478
BciT130I CCWGG 1 cut(s) 603
BciVI GTATCC 1 cut(s) 897
BclI TGATCA 1 cut(s) 1032
BcoDI GTCTC 1 cut(s) 935
BfaI CTAG 1 cut(s) 521
BfuI GTATCC 1 cut(s) 897
BglII AGATCT 1 cut(s) 505
BisI GCNGC 2 cut(s) 108, 498
BlsI GCNGC 2 cut(s) 109, 499
BmcAI AGTACT 1 cut(s) 891
Bme1390I CCNGG 1 cut(s) 603
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 3 cut(s) 266, 421, 918
BmrFI CCNGG 1 cut(s) 603
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 3 cut(s) 592, 640, 819
BmuI ACTGGG 1 cut(s) 20
BplI GAGNNNNNCTC 4 cut(s) 818, 850, 908, 940
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 3 cut(s) 353, 792, 910
BsaBI GATNNNNATC 1 cut(s) 1020
BsaWI WCCGGW 1 cut(s) 233
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 1 cut(s) 458
Bse1I ACTGG 3 cut(s) 15, 223, 663
Bse3DI GCAATG 1 cut(s) 849
Bse8I GATNNNNATC 1 cut(s) 1020
BseBI CCWGG 1 cut(s) 603
BseGI GGATG 1 cut(s) 583
BseJI GATNNNNATC 1 cut(s) 1020
BseLI CCNNNNNNNGG 1 cut(s) 458
BseMI GCAATG 1 cut(s) 849
BseMII CTCAG 2 cut(s) 153, 816
BseNI ACTGG 3 cut(s) 15, 223, 663
BseRI GAGGAG 2 cut(s) 446, 722
BseXI GCAGC 1 cut(s) 119
BseYI CCCAGC 2 cut(s) 920, 1025
BsgI GTGCAG 2 cut(s) 350, 1009
BshNI GGYRCC 1 cut(s) 419
BsiSI CCGG 1 cut(s) 234
BslI CCNNNNNNNGG 1 cut(s) 458
BsmAI GTCTC 1 cut(s) 935
Bsp1286I GDGCHC 1 cut(s) 921
Bsp143I GATC 5 cut(s) 505, 605, 728, 976, 1032
BspACI CCGC 2 cut(s) 497, 1103
BspCNI CTCAG 2 cut(s) 154, 817
BspLI GGNNCC 3 cut(s) 266, 421, 918
BspPI GGATC 1 cut(s) 613
BspT107I GGYRCC 1 cut(s) 419
BsrBI CCGCTC 1 cut(s) 497
BsrDI GCAATG 1 cut(s) 849
BsrI ACTGG 3 cut(s) 15, 223, 663
BssMI GATC 5 cut(s) 505, 605, 728, 976, 1032
BssNAI GTATAC 1 cut(s) 756
Bst1107I GTATAC 1 cut(s) 756
Bst2UI CCWGG 1 cut(s) 603
Bst4CI ACNGT 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 1003
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 3 cut(s) 162, 593, 825
BstF5I GGATG 1 cut(s) 583
BstKTI GATC 5 cut(s) 508, 608, 731, 979, 1035
BstMAI GTCTC 1 cut(s) 935
BstMBI GATC 5 cut(s) 505, 605, 728, 976, 1032
BstMWI GCNNNNNNNGC 4 cut(s) 104, 517, 575, 1003
BstNI CCWGG 1 cut(s) 603
BstNSI RCATGY 2 cut(s) 122, 488
BstSCI CCNGG 1 cut(s) 601
BstV1I GCAGC 1 cut(s) 119
BstX2I RGATCY 2 cut(s) 505, 605
BstXI CCANNNNNNTGG 1 cut(s) 732
BstYI RGATCY 2 cut(s) 505, 605
BstZ17I GTATAC 1 cut(s) 756
BsuI GTATCC 1 cut(s) 897
BtsCI GGATG 1 cut(s) 583
Cac8I GCNNGC 1 cut(s) 120
Cfr13I GGNCC 1 cut(s) 17
Csp6I GTAC 3 cut(s) 722, 890, 1119
CviAII CATG 8 cut(s) 43, 119, 338, 424, 445, 485, 517, 895
CviQI GTAC 3 cut(s) 722, 890, 1119
DdeI CTNAG 3 cut(s) 162, 593, 825
DpnI GATC 5 cut(s) 507, 607, 730, 978, 1034
DpnII GATC 5 cut(s) 505, 605, 728, 976, 1032
DraI TTTAAA 1 cut(s) 675
EciI GGCGGA 1 cut(s) 1092
Eco24I GRGCYC 1 cut(s) 921
Eco47I GGWCC 1 cut(s) 17
EcoRII CCWGG 1 cut(s) 601
EcoT22I ATGCAT 1 cut(s) 900
EcoT38I GRGCYC 1 cut(s) 921
FaeI CATG 8 cut(s) 46, 122, 341, 427, 448, 488, 520, 898
FalI AAGNNNNNCTT 2 cut(s) 124, 156
FatI CATG 8 cut(s) 42, 118, 337, 423, 444, 484, 516, 894
FbaI TGATCA 1 cut(s) 1032
FblI GTMKAC 3 cut(s) 405, 463, 755
Fnu4HI GCNGC 2 cut(s) 108, 498
FokI GGATG 1 cut(s) 570
FriOI GRGCYC 1 cut(s) 921
Fsp4HI GCNGC 2 cut(s) 108, 498
FspBI CTAG 1 cut(s) 521
GluI GCNGC 2 cut(s) 108, 498
GsaI CCCAGC 2 cut(s) 924, 1029
HapII CCGG 1 cut(s) 234
Hin1II CATG 8 cut(s) 46, 122, 341, 427, 448, 488, 520, 898
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 5 cut(s) 621, 647, 805, 911, 1083
HpaII CCGG 1 cut(s) 234
HphI GGTGA 1 cut(s) 65
Hpy166II GTNNAC 5 cut(s) 137, 406, 464, 756, 772
Hpy188I TCNGA 3 cut(s) 73, 328, 916
Hpy188III TCNNGA 5 cut(s) 101, 205, 376, 644, 809
Hpy8I GTNNAC 5 cut(s) 137, 406, 464, 756, 772
HpyAV CCTTC 3 cut(s) 380, 486, 1081
HpyCH4III ACNGT 1 cut(s) 189
HpyCH4V TGCA 6 cut(s) 118, 213, 275, 367, 898, 990
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 517, 575, 1003
HpyF3I CTNAG 3 cut(s) 162, 593, 825
Hsp92II CATG 8 cut(s) 46, 122, 341, 427, 448, 488, 520, 898
Ksp22I TGATCA 1 cut(s) 1032
Kzo9I GATC 5 cut(s) 505, 605, 728, 976, 1032
LmnI GCTCC 4 cut(s) 433, 494, 556, 916
Lsp1109I GCAGC 1 cut(s) 119
LweI GCATC 3 cut(s) 592, 640, 819
MaeI CTAG 1 cut(s) 521
MaeIII GTNAC 1 cut(s) 837
MalI GATC 5 cut(s) 507, 607, 730, 978, 1034
MbiI CCGCTC 1 cut(s) 497
MboI GATC 5 cut(s) 505, 605, 728, 976, 1032
MboII GAAGA 5 cut(s) 642, 645, 675, 1059, 1078
MflI RGATCY 2 cut(s) 505, 605
MhlI GDGCHC 1 cut(s) 921
MluCI AATT 4 cut(s) 33, 198, 676, 961
MlyI GAGTC 1 cut(s) 799
Mph1103I ATGCAT 1 cut(s) 900
MseI TTAA 1 cut(s) 674
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 1 cut(s) 603
MvaI CCWGG 1 cut(s) 603
MwoI GCNNNNNNNGC 4 cut(s) 104, 517, 575, 1003
NdeII GATC 5 cut(s) 505, 605, 728, 976, 1032
NlaIII CATG 8 cut(s) 46, 122, 341, 427, 448, 488, 520, 898
NlaIV GGNNCC 3 cut(s) 266, 421, 918
NsiI ATGCAT 1 cut(s) 900
NspI RCATGY 2 cut(s) 122, 488
PaeI GCATGC 1 cut(s) 122
PciI ACATGT 1 cut(s) 484
PfeI GAWTC 4 cut(s) 621, 647, 911, 1083
PkrI GCNGC 2 cut(s) 109, 499
PleI GAGTC 1 cut(s) 799
PpsI GAGTC 1 cut(s) 799
PscI ACATGT 1 cut(s) 484
Psp6I CCWGG 1 cut(s) 601
PspFI CCCAGC 2 cut(s) 920, 1025
PspGI CCWGG 1 cut(s) 601
PspN4I GGNNCC 3 cut(s) 266, 421, 918
PspPI GGNCC 1 cut(s) 17
PsuI RGATCY 2 cut(s) 505, 605
RsaI GTAC 3 cut(s) 723, 891, 1120
RsaNI GTAC 3 cut(s) 722, 890, 1119
SaqAI TTAA 1 cut(s) 674
SatI GCNGC 2 cut(s) 108, 498
Sau3AI GATC 5 cut(s) 505, 605, 728, 976, 1032
Sau96I GGNCC 1 cut(s) 17
ScaI AGTACT 1 cut(s) 891
SchI GAGTC 1 cut(s) 799
ScrFI CCNGG 1 cut(s) 603
SduI GDGCHC 1 cut(s) 921
SfaNI GCATC 3 cut(s) 592, 640, 819
SinI GGWCC 1 cut(s) 17
SmlI CTYRAG 3 cut(s) 332, 807, 925
SmoI CTYRAG 3 cut(s) 332, 807, 925
SphI GCATGC 1 cut(s) 122
Sse9I AATT 4 cut(s) 33, 198, 676, 961
SsiI CCGC 2 cut(s) 497, 1103
SspI AATATT 1 cut(s) 1011
SspMI CTAG 1 cut(s) 521
StyD4I CCNGG 1 cut(s) 601
TaaI ACNGT 1 cut(s) 189
TaqI TCGA 1 cut(s) 243
TasI AATT 4 cut(s) 33, 198, 676, 961
TatI WGTACW 1 cut(s) 889
TauI GCSGC 1 cut(s) 500
TfiI GAWTC 4 cut(s) 621, 647, 911, 1083
Tru1I TTAA 1 cut(s) 674
Tru9I TTAA 1 cut(s) 674
TseI GCWGC 1 cut(s) 107
TspDTI ATGAA 6 cut(s) 17, 354, 433, 645, 884, 1063
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 2 cut(s) 33, 676
XceI RCATGY 2 cut(s) 122, 488
XmiI GTMKAC 3 cut(s) 405, 463, 755
XspI CTAG 1 cut(s) 521
ZrmI AGTACT 1 cut(s) 891
Zsp2I ATGCAT 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.