RLG00000036289

tRNA pseudouridine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007446
Physical Location & Seq
Reverse (-)
78482818 .. 78485388
2571 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036289

Sequence Viewer

Length: 1539 bp
ATGGCTCTGTCTTCACTGCGACTCCCGGTCTCGCTTTGGCTTCCAAAACACCCAAACCAGAGCTTCCATTTTTCAAACCCAAAAATACCCTCACGATTGAGCCTTATTTACTGCTGCTCCTCCTCCGCTGCCACTGCTCAACAAACCTTAACCCGCACTGAAAAATGGCAACCCGTTCGAAAGAAGAAAGTGGTTCTCCGGGTCGGGTATGTGGGCACCGATTACCGAGGTCTGCAAATGCAAAGAGATGACCCTTCAATATCTACTATTGAAAAGGAGCTGGAAACTGCCATATTCAAGGCCGGTGGTATTCGCGAGAGTAATTTCGGGAACCTGAACAAGATTGCGTGGGCCAGAAGTAGCAGAACCGATAAGGGAGTTCACTCCTTGGCGACAATGATATCGTTGAAAATGGAAATCCCTCAAAATGCGTGGGACGGAGACCCTTATGGCATTGCTCTTGTGAATCACATCAACTGTTACCTTCCTCATAATATCAAAATTTTTAGTGTTTTACCTGCACAGAGGAGCTTTGATCCCAGAAAGGAGTGTAATCTCCGTATGTATTCATACCTCCTTCCTGCTGATGTTATTGGAATCAAAAGTCACTTTAGCACAGCTGAAATTGATGATCACATTGCAGACTTCAACGATATACTAAATGCCTTCGAGGGTGAACATCCTTTTCATAACTATACAGTACGATCTAAATACAGGAAAAAACTTCCTGCGAAGAAATCACCTATATACTGCAATATGTCAAAAACAGAAAAATCAACTGTTGAGGCATCAGCCTCCGAGCTTGAGGGAATTGACGATGAAGATGAGTTCGAGGGAGAAAATACATCAGAAACTGAGGTACTGATCCAAAATTCTTTCCTGTCCAACAATGGGAATGAAAACTTACTGAAAGCATGCAAAAAGCAGGAAGATGTTCTGAAGGAGAAAAGTACTGATACAGTTATCCGTGCAAAATGGTTATACGAACCTGATGAGAGCGATAGAATCGGTGCTTCTCATTTCAGAAAGATATTTCGCTGTTCTTGTGGAAAGCTTGAAAAATCGCTGGCACATGAGTATGTTGAGCTCTCCATATGGGGAGAGTCTTTCATGTTACATCAAATTCGCAAAATGGTTGGAACTGCTGTGGCCATAAAGCGCAAGTTACTCCCAAGAGATATTCTGACATTATCACTGGCCAAGTTCTCACGGATAATCCTACCCCTTGCTCCATCTGAAGTTTTGATCTTAAGAGGAAACAGATTTTCAATTAGAGAAAGACCTGGAAATGTAACAAGGCCTGAAATGATTGCAATGGTTGAATCAGAAGAAATTATCAAACAGGTTGATGAGTTCTTCACATCTGTAATGTTGCCTCAAGTTGCAAAGTTCTTAGAACCTTCAGGATATCCTTGGCAAGAATGGATTGAAAACTTGGATAGAAACACAGGTATTCCAGATATAGAATTGGATGAAGTTAGAAATGCTTGGAAAGTGTGGAAGGAAAGGTTTGAGAGTGATGCATCAATCCTAAAGTAG

Protein Analysis

513

Amino Acids

58.53

Weight (kDa)

7.99

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_1 PF01416 338 - 414 8.8e-06 tRNA pseudouridine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 526
AccB1I GGYRCC 1 cut(s) 215
AccII CGCG 1 cut(s) 315
AciI CCGC 2 cut(s) 126, 154
AclWI GGATC 2 cut(s) 530, 859
AcoI YGGCCR 2 cut(s) 1149, 1197
AcsI RAATTY 3 cut(s) 501, 871, 1122
AcuI CTGAAG 3 cut(s) 959, 1257, 1386
AfaI GTAC 3 cut(s) 702, 861, 952
AfiI CCNNNNNNNGG 1 cut(s) 891
AflII CTTAAG 1 cut(s) 1249
AhdI GACNNNNNGTC 1 cut(s) 26
AjnI CCWGG 1 cut(s) 1282
AluBI AGCT 7 cut(s) 63, 280, 531, 620, 802, 1054, 1087
AluI AGCT 7 cut(s) 63, 280, 531, 620, 802, 1054, 1087
Alw21I GWGCWC 1 cut(s) 1089
Alw26I GTCTC 2 cut(s) 34, 435
AlwI GGATC 2 cut(s) 530, 859
AlwNI CAGNNNCTG 1 cut(s) 854
AoxI GGCC 5 cut(s) 300, 351, 1149, 1197, 1298
ApeKI GCWGC 2 cut(s) 114, 128
ApoI RAATTY 3 cut(s) 501, 871, 1122
Asp700I GAANNNNTTC 1 cut(s) 933
AspLEI GCGC 1 cut(s) 1161
AspS9I GGNCC 1 cut(s) 351
AsuC2I CCSGG 2 cut(s) 26, 200
AsuHPI GGTGA 2 cut(s) 686, 732
AsuII TTCGAA 1 cut(s) 178
BaeGI GKGCMC 1 cut(s) 218
BalI TGGCCA 2 cut(s) 1151, 1199
BanI GGYRCC 1 cut(s) 215
BanII GRGCYC 1 cut(s) 1089
BbsI GAAGAC 1 cut(s) 3
Bbv12I GWGCWC 1 cut(s) 1089
BbvI GCAGC 2 cut(s) 101, 115
BccI CCATC 1 cut(s) 1240
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 1 cut(s) 1284
BclI TGATCA 1 cut(s) 631
BcnI CCSGG 2 cut(s) 26, 200
BcoDI GTCTC 2 cut(s) 34, 435
BfrI CTTAAG 1 cut(s) 1249
BfuAI ACCTGC 1 cut(s) 526
BisI GCNGC 2 cut(s) 115, 129
BlsI GCNGC 2 cut(s) 116, 130
BmcAI AGTACT 1 cut(s) 952
Bme1390I CCNGG 3 cut(s) 26, 200, 1284
BmeRI GACNNNNNGTC 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 351
BmiI GGNNCC 2 cut(s) 217, 332
BmrFI CCNGG 3 cut(s) 26, 200, 1284
BmsI GCATC 3 cut(s) 797, 1510, 1532
BpiI GAAGAC 1 cut(s) 3
Bpu14I TTCGAA 1 cut(s) 178
BpuEI CTTGAG 2 cut(s) 824, 1362
BpuMI CCSGG 2 cut(s) 26, 200
BsaI GGTCTC 2 cut(s) 34, 435
BsaJI CCNNGG 3 cut(s) 226, 387, 1412
BsaXI ACNNNNNCTCC 4 cut(s) 6, 36, 101, 131
Bsc4I CCNNNNNNNGG 1 cut(s) 891
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 1 cut(s) 1200
Bse3DI GCAATG 3 cut(s) 453, 636, 1320
BseBI CCWGG 1 cut(s) 1284
BseDI CCNNGG 3 cut(s) 226, 387, 1412
BseGI GGATG 2 cut(s) 679, 1477
BseLI CCNNNNNNNGG 1 cut(s) 891
BseMI GCAATG 3 cut(s) 453, 636, 1320
BseMII CTCAG 1 cut(s) 846
BseNI ACTGG 1 cut(s) 1200
BseRI GAGGAG 3 cut(s) 109, 112, 541
BseSI GKGCMC 1 cut(s) 218
BseXI GCAGC 2 cut(s) 101, 115
BsgI GTGCAG 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 315
BshFI GGCC 5 cut(s) 302, 353, 1151, 1199, 1300
BshNI GGYRCC 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 1089
BsiSI CCGG 3 cut(s) 26, 199, 303
BslFI GGGAC 1 cut(s) 449
BslI CCNNNNNNNGG 1 cut(s) 891
BsmAI GTCTC 2 cut(s) 34, 435
BsmFI GGGAC 1 cut(s) 449
BsnI GGCC 5 cut(s) 302, 353, 1151, 1199, 1300
Bso31I GGTCTC 2 cut(s) 34, 435
Bsp119I TTCGAA 1 cut(s) 178
Bsp1286I GDGCHC 2 cut(s) 218, 1089
Bsp143I GATC 5 cut(s) 535, 631, 704, 864, 1245
Bsp68I TCGCGA 1 cut(s) 315
BspACI CCGC 2 cut(s) 126, 154
BspANI GGCC 5 cut(s) 302, 353, 1151, 1199, 1300
BspCNI CTCAG 1 cut(s) 847
BspFNI CGCG 1 cut(s) 315
BspLI GGNNCC 2 cut(s) 217, 332
BspMI ACCTGC 1 cut(s) 526
BspPI GGATC 2 cut(s) 530, 859
BspT104I TTCGAA 1 cut(s) 178
BspT107I GGYRCC 1 cut(s) 215
BspTI CTTAAG 1 cut(s) 1249
BspTNI GGTCTC 2 cut(s) 34, 435
BsrDI GCAATG 3 cut(s) 453, 636, 1320
BsrFI RCCGGY 1 cut(s) 302
BsrI ACTGG 1 cut(s) 1200
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 3 cut(s) 226, 387, 1412
BssMI GATC 5 cut(s) 535, 631, 704, 864, 1245
BssT1I CCWWGG 2 cut(s) 387, 1412
Bst2UI CCWGG 1 cut(s) 1284
Bst4CI ACNGT 4 cut(s) 479, 700, 781, 961
BstAFI CTTAAG 1 cut(s) 1249
BstBI TTCGAA 1 cut(s) 178
BstC8I GCNNGC 2 cut(s) 916, 1068
BstDEI CTNAG 2 cut(s) 855, 1393
BstF5I GGATG 2 cut(s) 679, 1477
BstFNI CGCG 1 cut(s) 315
BstHHI GCGC 1 cut(s) 1161
BstKTI GATC 5 cut(s) 538, 634, 707, 867, 1248
BstMAI GTCTC 2 cut(s) 34, 435
BstMBI GATC 5 cut(s) 535, 631, 704, 864, 1245
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 1 cut(s) 1284
BstNSI RCATGY 1 cut(s) 918
BstSCI CCNGG 3 cut(s) 24, 198, 1282
BstSLI GKGCMC 1 cut(s) 218
BstUI CGCG 1 cut(s) 315
BstV1I GCAGC 2 cut(s) 101, 115
BstV2I GAAGAC 1 cut(s) 3
BsuRI GGCC 5 cut(s) 302, 353, 1151, 1199, 1300
BtsCI GGATG 2 cut(s) 679, 1477
BtsI GCAGTG 2 cut(s) 14, 132
BtsIMutI CAGTG 4 cut(s) 14, 132, 156, 1193
BtuMI TCGCGA 1 cut(s) 315
BveI ACCTGC 1 cut(s) 526
Cac8I GCNNGC 2 cut(s) 916, 1068
CaiI CAGNNNCTG 1 cut(s) 854
CfoI GCGC 1 cut(s) 1161
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 1 cut(s) 351
Csp6I GTAC 3 cut(s) 701, 860, 951
CspCI CAANNNNNGTGG 4 cut(s) 286, 321, 413, 448
CviAII CATG 3 cut(s) 915, 1073, 1111
CviQI GTAC 3 cut(s) 701, 860, 951
DdeI CTNAG 2 cut(s) 855, 1393
DpnI GATC 5 cut(s) 537, 633, 706, 866, 1247
DpnII GATC 5 cut(s) 535, 631, 704, 864, 1245
DriI GACNNNNNGTC 1 cut(s) 26
EaeI YGGCCR 2 cut(s) 1149, 1197
Eam1105I GACNNNNNGTC 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 1087
Eco130I CCWWGG 2 cut(s) 387, 1412
Eco147I AGGCCT 1 cut(s) 1300
Eco24I GRGCYC 1 cut(s) 1089
Eco31I GGTCTC 2 cut(s) 34, 435
Eco32I GATATC 2 cut(s) 402, 1409
Eco53kI GAGCTC 1 cut(s) 1087
Eco57I CTGAAG 3 cut(s) 959, 1257, 1386
EcoICRI GAGCTC 1 cut(s) 1087
EcoRII CCWGG 1 cut(s) 1282
EcoRV GATATC 2 cut(s) 402, 1409
EcoT14I CCWWGG 2 cut(s) 387, 1412
EcoT22I ATGCAT 1 cut(s) 1525
EcoT38I GRGCYC 1 cut(s) 1089
ErhI CCWWGG 2 cut(s) 387, 1412
FaeI CATG 3 cut(s) 918, 1076, 1114
FaqI GGGAC 1 cut(s) 449
FatI CATG 3 cut(s) 914, 1072, 1110
FauI CCCGC 1 cut(s) 161
FauNDI CATATG 1 cut(s) 1094
FbaI TGATCA 1 cut(s) 631
Fnu4HI GCNGC 2 cut(s) 115, 129
FokI GGATG 2 cut(s) 666, 1484
FriOI GRGCYC 1 cut(s) 1089
Fsp4HI GCNGC 2 cut(s) 115, 129
GlaI GCGC 1 cut(s) 1160
GluI GCNGC 2 cut(s) 115, 129
HaeIII GGCC 5 cut(s) 302, 353, 1151, 1199, 1300
HapII CCGG 3 cut(s) 26, 199, 303
HhaI GCGC 1 cut(s) 1161
Hin1II CATG 3 cut(s) 918, 1076, 1114
Hin6I GCGC 1 cut(s) 1159
HinP1I GCGC 1 cut(s) 1159
HindIII AAGCTT 1 cut(s) 1052
HinfI GANTC 6 cut(s) 21, 466, 597, 1005, 1103, 1322
HpaII CCGG 3 cut(s) 26, 199, 303
HphI GGTGA 2 cut(s) 686, 732
Hpy166II GTNNAC 2 cut(s) 382, 677
Hpy188I TCNGA 7 cut(s) 799, 850, 939, 1025, 1185, 1237, 1327
Hpy188III TCNNGA 5 cut(s) 93, 314, 328, 1404, 1457
Hpy8I GTNNAC 2 cut(s) 382, 677
HpyAV CCTTC 7 cut(s) 264, 494, 587, 676, 934, 1410, 1495
HpyCH4III ACNGT 4 cut(s) 479, 700, 781, 961
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 2 cut(s) 855, 1393
Hsp92II CATG 3 cut(s) 918, 1076, 1114
HspAI GCGC 1 cut(s) 1159
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 5 cut(s) 535, 631, 704, 864, 1245
LmnI GCTCC 4 cut(s) 122, 277, 528, 1234
Lsp1109I GCAGC 2 cut(s) 101, 115
LweI GCATC 3 cut(s) 797, 1510, 1532
MaeIII GTNAC 5 cut(s) 479, 605, 1113, 1164, 1291
MalI GATC 5 cut(s) 537, 633, 706, 866, 1247
MboI GATC 5 cut(s) 535, 631, 704, 864, 1245
MboII GAAGA 7 cut(s) 3, 196, 745, 833, 941, 1340, 1348
MhlI GDGCHC 2 cut(s) 218, 1089
MlsI TGGCCA 2 cut(s) 1151, 1199
MluCI AATT 9 cut(s) 322, 501, 624, 810, 871, 1122, 1269, 1332, 1466
MluNI TGGCCA 2 cut(s) 1151, 1199
MlyI GAGTC 2 cut(s) 15, 1112
MmeI TCCRAC 2 cut(s) 909, 1117
Mox20I TGGCCA 2 cut(s) 1151, 1199
Mph1103I ATGCAT 1 cut(s) 1525
MroXI GAANNNNTTC 1 cut(s) 933
MscI TGGCCA 2 cut(s) 1151, 1199
MseI TTAA 2 cut(s) 149, 1250
MslI CAYNNNNRTG 1 cut(s) 1077
Msp20I TGGCCA 2 cut(s) 1151, 1199
MspA1I CMGCKG 2 cut(s) 128, 620
MspCI CTTAAG 1 cut(s) 1249
MspI CCGG 3 cut(s) 26, 199, 303
MspR9I CCNGG 3 cut(s) 26, 200, 1284
MvaI CCWGG 1 cut(s) 1284
MvnI CGCG 1 cut(s) 315
MwoI GCNNNNNNNGC 1 cut(s) 134
NciI CCSGG 2 cut(s) 26, 200
NdeI CATATG 1 cut(s) 1094
NdeII GATC 5 cut(s) 535, 631, 704, 864, 1245
NlaIII CATG 3 cut(s) 918, 1076, 1114
NlaIV GGNNCC 2 cut(s) 217, 332
NmuCI GTSAC 1 cut(s) 605
NruI TCGCGA 1 cut(s) 315
NsiI ATGCAT 1 cut(s) 1525
NspI RCATGY 1 cut(s) 918
NspV TTCGAA 1 cut(s) 178
PaeI GCATGC 1 cut(s) 918
PceI AGGCCT 1 cut(s) 1300
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 4 cut(s) 466, 597, 1005, 1322
PkrI GCNGC 2 cut(s) 116, 130
PleI GAGTC 2 cut(s) 15, 1111
PpsI GAGTC 2 cut(s) 15, 1111
Psp124BI GAGCTC 1 cut(s) 1089
Psp6I CCWGG 1 cut(s) 1282
PspGI CCWGG 1 cut(s) 1282
PspN4I GGNNCC 2 cut(s) 217, 332
PspPI GGNCC 1 cut(s) 351
PstNI CAGNNNCTG 1 cut(s) 854
PvuII CAGCTG 1 cut(s) 620
RruI TCGCGA 1 cut(s) 315
RsaI GTAC 3 cut(s) 702, 861, 952
RsaNI GTAC 3 cut(s) 701, 860, 951
RseI CAYNNNNRTG 1 cut(s) 1077
SacI GAGCTC 1 cut(s) 1089
SaqAI TTAA 2 cut(s) 149, 1250
SatI GCNGC 2 cut(s) 115, 129
Sau3AI GATC 5 cut(s) 535, 631, 704, 864, 1245
Sau96I GGNCC 1 cut(s) 351
ScaI AGTACT 1 cut(s) 952
SchI GAGTC 2 cut(s) 15, 1112
ScrFI CCNGG 3 cut(s) 26, 200, 1284
SduI GDGCHC 2 cut(s) 218, 1089
SfaNI GCATC 3 cut(s) 797, 1510, 1532
SfuI TTCGAA 1 cut(s) 178
SmiMI CAYNNNNRTG 1 cut(s) 1077
SmlI CTYRAG 3 cut(s) 803, 1249, 1377
SmoI CTYRAG 3 cut(s) 803, 1249, 1377
SphI GCATGC 1 cut(s) 918
Sse9I AATT 9 cut(s) 322, 501, 624, 810, 871, 1122, 1269, 1332, 1466
SseBI AGGCCT 1 cut(s) 1300
SsiI CCGC 2 cut(s) 126, 154
SstI GAGCTC 1 cut(s) 1089
StuI AGGCCT 1 cut(s) 1300
StyD4I CCNGG 3 cut(s) 24, 198, 1282
StyI CCWWGG 2 cut(s) 387, 1412
TaaI ACNGT 4 cut(s) 479, 700, 781, 961
TaqI TCGA 3 cut(s) 178, 669, 831
TasI AATT 9 cut(s) 322, 501, 624, 810, 871, 1122, 1269, 1332, 1466
TatI WGTACW 1 cut(s) 950
TfiI GAWTC 4 cut(s) 466, 597, 1005, 1322
Tru1I TTAA 2 cut(s) 149, 1250
Tru9I TTAA 2 cut(s) 149, 1250
TscAI CASTG 4 cut(s) 21, 139, 163, 1200
TseFI GTSAC 1 cut(s) 605
TseI GCWGC 2 cut(s) 114, 128
Tsp45I GTSAC 1 cut(s) 605
TspDTI ATGAA 6 cut(s) 558, 677, 834, 912, 1099, 1488
TspGWI ACGGA 4 cut(s) 453, 548, 956, 1225
TspRI CASTG 4 cut(s) 21, 139, 163, 1200
Vha464I CTTAAG 1 cut(s) 1249
XapI RAATTY 3 cut(s) 501, 871, 1122
XceI RCATGY 1 cut(s) 918
XmnI GAANNNNTTC 1 cut(s) 933
ZrmI AGTACT 1 cut(s) 952
Zsp2I ATGCAT 1 cut(s) 1525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.