Rh5DG505300

tRNA pseudouridine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007446
Physical Location & Seq
Reverse (-)
79069660 .. 79072867
3208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG505300.1

Sequence Viewer

Length: 1302 bp
ATGCAAAGAGATGACCCTTCAATATCTACTATTGAAAAGGAACTGGAAACTGCCATATTCAAGGCCGGTGGTATTCGCGAGAGTAACTTCGGGAACCTGAACAAGATTGCGTGGGCCAGAAGCAGCAGAACTGATAAGGGAGTTCACTCCTTGGCGACAATGATATCATTGAAAATGGAAATCCCTGAAAATGCGTGGGACGGAGACCCGTATGGCATTGCTCTTGTGAATCACATCAACTGTTACCTTCCTCACAATATCAAAATTTTTAGTGTTTTACCTGCACAGAGGAGCTTTGATCCCAGAAAGGAGTGTAATCTCCGTATGTATTCATACCTCCTTCCTGCTGATGTTATTGGAATCAAAAGTCACTTTAGCACAGCTGAAATTGATGATCACATTGCAGACTTCAACGATATACTAAATGCTTTCGAGGGTGAACATCCTTTTCATAACTATACAGTACGATCTAAATACCGGAAAAAACTTCCTGGGAAGAAATCACCTATATACCGCAATATGTCAAACACAGAAAGATCAACTGTTGAGGCATCAGCCTCCGAGCTTGAGGGAAGTGACGATGAAGATGAGTTCGAGGGAGAAAATACATCAGAAACTGAGGTACTGAACCAAAATTCTTTCCTGTCCAGCAATGGGAATGAAAACTTACTGAAAGCATGCAAGAAGCAGGAAGATGTTCTGAAGGAGAAAAGTACTGATTCAGTTATCCGTGCAAAATGGTTATACGAACCTGATGAGAGCGATAGAATCGGTGCTTCTCATTTCAGAAAGATATTTCGCTGTTCTTGTGGAAAGCTTGAAAAATCGCTGGCACATGAGTATGTTGAGCTCTCCATATGGGGAGAGTCTTTCATGTTACATCAAATTCGCAAAATGGTTGGAACTGCTGTGGCCATAAAGCGCAAGTTACTCCCAAGAGATATTCTGACATTATCACTGGCCAAGTTCTCACGGATAATCCTACCCCTTGCTCCATCTGAAGTTTTGATCTTAAGAGGAAACAGATTTTCAATTAGAGAAAGACCTGGAAATGTAACAAGGCCTGAAATGATTGCAATGGTTGAATCAGAAGAAATTATCAAACAGGTTGATGAGTTCTTCACATCTGTAATGTTGCCTCAAGTTGCAAAGTTCTTAGAACCTTCAGGATATCCTTGGCAAGAATGGATTGAAAACTTGGATAGAAACACAGGTATTCCAGATATAGAATTGGATGAAGTTAGAAATGCTTGGAAAGTGTGGAAGGAAAGGTTTGAGAGTGATGCATCAATCCTAAAGTAA

Protein Analysis

433

Amino Acids

49.56

Weight (kDa)

5.73

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_1 PF01416 259 - 335 7.9e-06 tRNA pseudouridine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 289
AccII CGCG 1 cut(s) 78
AciI CCGC 1 cut(s) 514
AclWI GGATC 1 cut(s) 293
AcoI YGGCCR 2 cut(s) 912, 960
AcsI RAATTY 3 cut(s) 264, 634, 885
AcuI CTGAAG 3 cut(s) 722, 1020, 1149
AfaI GTAC 3 cut(s) 465, 624, 715
AfiI CCNNNNNNNGG 1 cut(s) 654
AflII CTTAAG 1 cut(s) 1012
AgsI TTSAA 9 cut(s) 21, 35, 61, 172, 412, 821, 1032, 1085, 1193
AjnI CCWGG 2 cut(s) 490, 1045
AluBI AGCT 5 cut(s) 294, 383, 565, 817, 850
AluI AGCT 5 cut(s) 294, 383, 565, 817, 850
Alw21I GWGCWC 1 cut(s) 852
Alw26I GTCTC 1 cut(s) 198
AlwI GGATC 1 cut(s) 293
AlwNI CAGNNNCTG 1 cut(s) 617
AoxI GGCC 5 cut(s) 63, 114, 912, 960, 1061
ApeKI GCWGC 1 cut(s) 123
ApoI RAATTY 3 cut(s) 264, 634, 885
Asp700I GAANNNNTTC 1 cut(s) 696
AspLEI GCGC 1 cut(s) 924
AspS9I GGNCC 1 cut(s) 114
AsuHPI GGTGA 2 cut(s) 449, 495
BalI TGGCCA 2 cut(s) 914, 962
BanII GRGCYC 1 cut(s) 852
Bbv12I GWGCWC 1 cut(s) 852
BbvI GCAGC 1 cut(s) 135
BccI CCATC 1 cut(s) 1003
BciT130I CCWGG 2 cut(s) 492, 1047
BclI TGATCA 1 cut(s) 394
BcoDI GTCTC 1 cut(s) 198
BfrI CTTAAG 1 cut(s) 1012
BfuAI ACCTGC 1 cut(s) 289
BisI GCNGC 1 cut(s) 124
BlsI GCNGC 1 cut(s) 125
BmcAI AGTACT 1 cut(s) 715
Bme1390I CCNGG 2 cut(s) 492, 1047
BmgT120I GGNCC 1 cut(s) 114
BmiI GGNNCC 1 cut(s) 95
BmrFI CCNGG 2 cut(s) 492, 1047
BmsI GCATC 3 cut(s) 560, 1273, 1295
BpuEI CTTGAG 2 cut(s) 587, 1125
BsaI GGTCTC 1 cut(s) 198
BsaJI CCNNGG 3 cut(s) 150, 491, 1175
BsaWI WCCGGW 1 cut(s) 477
Bsc4I CCNNNNNNNGG 1 cut(s) 654
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 2 cut(s) 48, 963
Bse3DI GCAATG 4 cut(s) 216, 399, 658, 1083
BseBI CCWGG 2 cut(s) 492, 1047
BseDI CCNNGG 3 cut(s) 150, 491, 1175
BseGI GGATG 2 cut(s) 442, 1240
BseLI CCNNNNNNNGG 1 cut(s) 654
BseMI GCAATG 4 cut(s) 216, 399, 658, 1083
BseMII CTCAG 1 cut(s) 609
BseNI ACTGG 2 cut(s) 48, 963
BseRI GAGGAG 1 cut(s) 304
BseXI GCAGC 1 cut(s) 135
BsgI GTGCAG 1 cut(s) 267
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 5 cut(s) 65, 116, 914, 962, 1063
BsiHKAI GWGCWC 1 cut(s) 852
BsiSI CCGG 2 cut(s) 66, 478
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 1 cut(s) 654
BsmAI GTCTC 1 cut(s) 198
BsmFI GGGAC 1 cut(s) 212
BsnI GGCC 5 cut(s) 65, 116, 914, 962, 1063
Bso31I GGTCTC 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 852
Bsp143I GATC 5 cut(s) 298, 394, 467, 536, 1008
Bsp68I TCGCGA 1 cut(s) 78
BspACI CCGC 1 cut(s) 514
BspANI GGCC 5 cut(s) 65, 116, 914, 962, 1063
BspCNI CTCAG 1 cut(s) 610
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 1 cut(s) 95
BspMI ACCTGC 1 cut(s) 289
BspPI GGATC 1 cut(s) 293
BspTI CTTAAG 1 cut(s) 1012
BspTNI GGTCTC 1 cut(s) 198
BsrDI GCAATG 4 cut(s) 216, 399, 658, 1083
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 2 cut(s) 48, 963
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 3 cut(s) 150, 491, 1175
BssMI GATC 5 cut(s) 298, 394, 467, 536, 1008
BssT1I CCWWGG 2 cut(s) 150, 1175
Bst2UI CCWGG 2 cut(s) 492, 1047
Bst4CI ACNGT 3 cut(s) 242, 463, 544
BstAFI CTTAAG 1 cut(s) 1012
BstC8I GCNNGC 2 cut(s) 679, 831
BstDEI CTNAG 2 cut(s) 618, 1156
BstF5I GGATG 2 cut(s) 442, 1240
BstFNI CGCG 1 cut(s) 78
BstHHI GCGC 1 cut(s) 924
BstKTI GATC 5 cut(s) 301, 397, 470, 539, 1011
BstMAI GTCTC 1 cut(s) 198
BstMBI GATC 5 cut(s) 298, 394, 467, 536, 1008
BstNI CCWGG 2 cut(s) 492, 1047
BstNSI RCATGY 1 cut(s) 681
BstSCI CCNGG 2 cut(s) 490, 1045
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 1 cut(s) 135
BsuRI GGCC 5 cut(s) 65, 116, 914, 962, 1063
BtsCI GGATG 2 cut(s) 442, 1240
BtsIMutI CAGTG 1 cut(s) 956
BtuMI TCGCGA 1 cut(s) 78
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 2 cut(s) 679, 831
CaiI CAGNNNCTG 1 cut(s) 617
CfoI GCGC 1 cut(s) 924
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 114
Csp6I GTAC 3 cut(s) 464, 623, 714
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 3 cut(s) 678, 836, 874
CviQI GTAC 3 cut(s) 464, 623, 714
DdeI CTNAG 2 cut(s) 618, 1156
DpnI GATC 5 cut(s) 300, 396, 469, 538, 1010
DpnII GATC 5 cut(s) 298, 394, 467, 536, 1008
EaeI YGGCCR 2 cut(s) 912, 960
Ecl136II GAGCTC 1 cut(s) 850
Eco130I CCWWGG 2 cut(s) 150, 1175
Eco147I AGGCCT 1 cut(s) 1063
Eco24I GRGCYC 1 cut(s) 852
Eco31I GGTCTC 1 cut(s) 198
Eco32I GATATC 2 cut(s) 165, 1172
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 3 cut(s) 722, 1020, 1149
EcoICRI GAGCTC 1 cut(s) 850
EcoRII CCWGG 2 cut(s) 490, 1045
EcoRV GATATC 2 cut(s) 165, 1172
EcoT14I CCWWGG 2 cut(s) 150, 1175
EcoT22I ATGCAT 1 cut(s) 1288
EcoT38I GRGCYC 1 cut(s) 852
ErhI CCWWGG 2 cut(s) 150, 1175
FaeI CATG 3 cut(s) 681, 839, 877
FaqI GGGAC 1 cut(s) 212
FatI CATG 3 cut(s) 677, 835, 873
FauNDI CATATG 1 cut(s) 857
FbaI TGATCA 1 cut(s) 394
Fnu4HI GCNGC 1 cut(s) 124
FokI GGATG 2 cut(s) 429, 1247
FriOI GRGCYC 1 cut(s) 852
Fsp4HI GCNGC 1 cut(s) 124
GlaI GCGC 1 cut(s) 923
GluI GCNGC 1 cut(s) 124
HaeIII GGCC 5 cut(s) 65, 116, 914, 962, 1063
HapII CCGG 2 cut(s) 66, 478
HhaI GCGC 1 cut(s) 924
Hin1II CATG 3 cut(s) 681, 839, 877
Hin6I GCGC 1 cut(s) 922
HinP1I GCGC 1 cut(s) 922
HindIII AAGCTT 1 cut(s) 815
HinfI GANTC 6 cut(s) 229, 360, 719, 768, 866, 1085
HpaII CCGG 2 cut(s) 66, 478
HphI GGTGA 2 cut(s) 449, 495
Hpy166II GTNNAC 2 cut(s) 145, 440
Hpy188I TCNGA 7 cut(s) 562, 613, 702, 788, 948, 1000, 1090
Hpy188III TCNNGA 4 cut(s) 77, 91, 1167, 1220
Hpy8I GTNNAC 2 cut(s) 145, 440
HpyAV CCTTC 6 cut(s) 27, 257, 350, 697, 1173, 1258
HpyCH4III ACNGT 3 cut(s) 242, 463, 544
HpyCH4V TGCA 8 cut(s) 4, 284, 404, 681, 734, 1076, 1148, 1286
HpyF3I CTNAG 2 cut(s) 618, 1156
Hsp92II CATG 3 cut(s) 681, 839, 877
HspAI GCGC 1 cut(s) 922
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 5 cut(s) 298, 394, 467, 536, 1008
LmnI GCTCC 2 cut(s) 291, 997
Lsp1109I GCAGC 1 cut(s) 135
LweI GCATC 3 cut(s) 560, 1273, 1295
MaeIII GTNAC 7 cut(s) 83, 242, 368, 575, 876, 927, 1054
MalI GATC 5 cut(s) 300, 396, 469, 538, 1010
MboI GATC 5 cut(s) 298, 394, 467, 536, 1008
MboII GAAGA 5 cut(s) 508, 596, 704, 1103, 1111
MhlI GDGCHC 1 cut(s) 852
MlsI TGGCCA 2 cut(s) 914, 962
MluCI AATT 7 cut(s) 264, 387, 634, 885, 1032, 1095, 1229
MluNI TGGCCA 2 cut(s) 914, 962
MlyI GAGTC 1 cut(s) 875
MmeI TCCRAC 1 cut(s) 880
Mox20I TGGCCA 2 cut(s) 914, 962
Mph1103I ATGCAT 1 cut(s) 1288
MroXI GAANNNNTTC 1 cut(s) 696
MscI TGGCCA 2 cut(s) 914, 962
MseI TTAA 1 cut(s) 1013
MslI CAYNNNNRTG 1 cut(s) 840
Msp20I TGGCCA 2 cut(s) 914, 962
MspA1I CMGCKG 1 cut(s) 383
MspCI CTTAAG 1 cut(s) 1012
MspI CCGG 2 cut(s) 66, 478
MspR9I CCNGG 2 cut(s) 492, 1047
MvaI CCWGG 2 cut(s) 492, 1047
MvnI CGCG 1 cut(s) 78
NdeI CATATG 1 cut(s) 857
NdeII GATC 5 cut(s) 298, 394, 467, 536, 1008
NlaIII CATG 3 cut(s) 681, 839, 877
NlaIV GGNNCC 1 cut(s) 95
NmuCI GTSAC 2 cut(s) 368, 575
NruI TCGCGA 1 cut(s) 78
NsiI ATGCAT 1 cut(s) 1288
NspI RCATGY 1 cut(s) 681
PaeI GCATGC 1 cut(s) 681
PceI AGGCCT 1 cut(s) 1063
PdmI GAANNNNTTC 1 cut(s) 696
PfeI GAWTC 5 cut(s) 229, 360, 719, 768, 1085
PkrI GCNGC 1 cut(s) 125
PleI GAGTC 1 cut(s) 874
PpsI GAGTC 1 cut(s) 874
Psp124BI GAGCTC 1 cut(s) 852
Psp6I CCWGG 2 cut(s) 490, 1045
PspGI CCWGG 2 cut(s) 490, 1045
PspN4I GGNNCC 1 cut(s) 95
PspPI GGNCC 1 cut(s) 114
PstNI CAGNNNCTG 1 cut(s) 617
PvuII CAGCTG 1 cut(s) 383
RruI TCGCGA 1 cut(s) 78
RsaI GTAC 3 cut(s) 465, 624, 715
RsaNI GTAC 3 cut(s) 464, 623, 714
RseI CAYNNNNRTG 1 cut(s) 840
SacI GAGCTC 1 cut(s) 852
SaqAI TTAA 1 cut(s) 1013
SatI GCNGC 1 cut(s) 124
Sau3AI GATC 5 cut(s) 298, 394, 467, 536, 1008
Sau96I GGNCC 1 cut(s) 114
ScaI AGTACT 1 cut(s) 715
SchI GAGTC 1 cut(s) 875
ScrFI CCNGG 2 cut(s) 492, 1047
SduI GDGCHC 1 cut(s) 852
SfaNI GCATC 3 cut(s) 560, 1273, 1295
SmiMI CAYNNNNRTG 1 cut(s) 840
SmlI CTYRAG 3 cut(s) 566, 1012, 1140
SmoI CTYRAG 3 cut(s) 566, 1012, 1140
SphI GCATGC 1 cut(s) 681
Sse9I AATT 7 cut(s) 264, 387, 634, 885, 1032, 1095, 1229
SseBI AGGCCT 1 cut(s) 1063
SsiI CCGC 1 cut(s) 514
SstI GAGCTC 1 cut(s) 852
StuI AGGCCT 1 cut(s) 1063
StyD4I CCNGG 2 cut(s) 490, 1045
StyI CCWWGG 2 cut(s) 150, 1175
TaaI ACNGT 3 cut(s) 242, 463, 544
TaqI TCGA 2 cut(s) 432, 594
TasI AATT 7 cut(s) 264, 387, 634, 885, 1032, 1095, 1229
TatI WGTACW 1 cut(s) 713
TfiI GAWTC 5 cut(s) 229, 360, 719, 768, 1085
Tru1I TTAA 1 cut(s) 1013
Tru9I TTAA 1 cut(s) 1013
TscAI CASTG 1 cut(s) 963
TseFI GTSAC 2 cut(s) 368, 575
TseI GCWGC 1 cut(s) 123
Tsp45I GTSAC 2 cut(s) 368, 575
TspDTI ATGAA 6 cut(s) 321, 440, 597, 675, 862, 1251
TspGWI ACGGA 4 cut(s) 216, 311, 719, 988
TspRI CASTG 1 cut(s) 963
Vha464I CTTAAG 1 cut(s) 1012
XapI RAATTY 3 cut(s) 264, 634, 885
XceI RCATGY 1 cut(s) 681
XmnI GAANNNNTTC 1 cut(s) 696
ZrmI AGTACT 1 cut(s) 715
Zsp2I ATGCAT 1 cut(s) 1288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.