Rorug05G0417300

tRNA pseudouridine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007446
Physical Location & Seq
Forward (+)
57988821 .. 57990344
1524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0417300.1

Sequence Viewer

Length: 927 bp
ATGGTGGGTAGGTTGTGGAGATACTTTCGAATTGATGGGGATCCACCGCAATACGCAGATCCCAATCTTTTCACTATGAGGATTCATCATGGGGGGAGATTTCATAGTGAGAGGGGACTGAATAGGATTTATAGAGGGGGCCAATTGTGCTATGTTGATGGAGTAGACCCGGACACAATGTCCATGACAGGGATACACTACTGGGCCTATGATTTGGGGTACCAAATGCCACCAATTCAATTTTGGTTTAGAATTCCTGGAAGTGAGGAGAGGGATGGCGAGCTTGATAAGTTTGAAGAAAATCTGAACCATGCTATGTTCATTGGCTGGTGGATTGATGATGTGGAGGAAGGACCCAATGTACCATCTGATGATGAGGAGGAAGAAGACTCATTGGGGGCTGAGGACTTGGGTCATGCTAAAGAGCCTATTTTTGTGGACTTGGATGACATAAGTGATGGGCCCAATGATGACACAGTTGATGCCAATAGACATGGAAGTGGGCCTGACCCATATGTGGATGGACATGGGCCTCAGGAACAGGATGCACAAGGACCTGATGAAAATGAGGTTGGACAGGGAGGTGAAGAAGAAATCGGGCATGGGCATGGGCCTCATAATGTTGAAGCATCCATGAATGAGGAGGAAGTGGAAAGAGCAAACAACAGAAGAAAGGACAAGGGCAAAGCCGTGGTTGAAGAAGATAGTCCTTCTGCAAAGAAGAATAAGGGCAGAAAAAGGAAGGAGGTGCAAATGAGGTATGCTACTAGGTCTGGAGGTAAGAGTTCAAGGAGGTACACTGTTCCAGATGTGGATTGCTCCTCTGAAAGCACAACAGATTCTGAGGACTCGGAGTTTGATGGGATAGTGGATTCAGATAATGACTTAAACAATGAGGATGATTGTCACGCCCCGGATTTTGAATGA

Protein Analysis

308

Amino Acids

34.72

Weight (kDa)

4.42

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1-like PF26130 23 - 96 9e-11 PB1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 219
AccB1I GGYRCC 1 cut(s) 219
AccI GTMKAC 1 cut(s) 165
AciI CCGC 1 cut(s) 47
AclWI GGATC 3 cut(s) 35, 48, 53
AcsI RAATTY 1 cut(s) 252
AfaI GTAC 3 cut(s) 221, 363, 797
AfiI CCNNNNNNNGG 2 cut(s) 189, 517
AgsI TTSAA 6 cut(s) 239, 296, 626, 698, 789, 923
AhdI GACNNNNNGTC 1 cut(s) 178
AjnI CCWGG 1 cut(s) 256
AjuI GAANNNNNNNTTGG 2 cut(s) 555, 587
AluBI AGCT 1 cut(s) 283
AluI AGCT 1 cut(s) 283
AlwI GGATC 3 cut(s) 35, 48, 53
AlwNI CAGNNNCTG 1 cut(s) 842
AoxI GGCC 6 cut(s) 139, 204, 461, 503, 530, 611
ApaI GGGCCC 1 cut(s) 465
ApoI RAATTY 1 cut(s) 252
ArsI GACNNNNNNTTYG 2 cut(s) 839, 871
Asp718I GGTACC 1 cut(s) 219
AspS9I GGNCC 9 cut(s) 139, 204, 353, 461, 462, 503, 530, 554, 611
AsuC2I CCSGG 2 cut(s) 170, 914
AsuHPI GGTGA 1 cut(s) 596
AsuII TTCGAA 1 cut(s) 28
AvaII GGWCC 2 cut(s) 353, 554
AxyI CCTNAGG 1 cut(s) 534
BaeGI GKGCMC 1 cut(s) 465
BamHI GGATCC 1 cut(s) 40
BanI GGYRCC 1 cut(s) 219
BanII GRGCYC 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 393
BbvCI CCTCAGC 1 cut(s) 402
BccI CCATC 7 cut(s) 29, 152, 269, 373, 452, 515, 854
BceAI ACGGC 1 cut(s) 674
BciT130I CCWGG 1 cut(s) 258
BciVI GTATCC 1 cut(s) 186
BcnI CCSGG 2 cut(s) 170, 914
BfaI CTAG 1 cut(s) 768
BfuI GTATCC 1 cut(s) 186
Bme1390I CCNGG 3 cut(s) 170, 258, 914
Bme18I GGWCC 2 cut(s) 353, 554
BmeRI GACNNNNNGTC 1 cut(s) 178
BmgT120I GGNCC 9 cut(s) 139, 204, 353, 461, 462, 503, 530, 554, 611
BmiI GGNNCC 5 cut(s) 42, 140, 221, 355, 463
BmrFI CCNGG 3 cut(s) 170, 258, 914
BmrI ACTGGG 1 cut(s) 211
BmsI GCATC 3 cut(s) 472, 535, 638
BmuI ACTGGG 1 cut(s) 211
BoxI GACNNNNGTC 1 cut(s) 411
BpiI GAAGAC 1 cut(s) 393
BpmI CTGGAG 1 cut(s) 795
Bpu10I CCTNAGC 1 cut(s) 402
Bpu14I TTCGAA 1 cut(s) 28
BpuMI CCSGG 2 cut(s) 170, 914
BsaBI GATNNNNATC 2 cut(s) 39, 63
BsaJI CCNNGG 2 cut(s) 690, 912
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 517
Bse1I ACTGG 1 cut(s) 206
Bse21I CCTNAGG 1 cut(s) 534
Bse8I GATNNNNATC 2 cut(s) 39, 63
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 2 cut(s) 690, 912
BseGI GGATG 6 cut(s) 280, 451, 526, 550, 629, 904
BseJI GATNNNNATC 2 cut(s) 39, 63
BseLI CCNNNNNNNGG 2 cut(s) 189, 517
BseMII CTCAG 3 cut(s) 393, 548, 834
BseNI ACTGG 1 cut(s) 206
BseRI GAGGAG 4 cut(s) 281, 392, 656, 811
BseSI GKGCMC 1 cut(s) 465
BshFI GGCC 6 cut(s) 141, 206, 463, 505, 532, 613
BshNI GGYRCC 1 cut(s) 219
BsiSI CCGG 2 cut(s) 170, 914
BslFI GGGAC 1 cut(s) 129
BslI CCNNNNNNNGG 2 cut(s) 189, 517
BsmFI GGGAC 1 cut(s) 129
BsnI GGCC 6 cut(s) 141, 206, 463, 505, 532, 613
Bsp119I TTCGAA 1 cut(s) 28
Bsp120I GGGCCC 1 cut(s) 461
Bsp1286I GDGCHC 1 cut(s) 465
Bsp143I GATC 2 cut(s) 40, 58
BspACI CCGC 1 cut(s) 47
BspANI GGCC 6 cut(s) 141, 206, 463, 505, 532, 613
BspCNI CTCAG 3 cut(s) 394, 547, 835
BspLI GGNNCC 5 cut(s) 42, 140, 221, 355, 463
BspPI GGATC 3 cut(s) 35, 48, 53
BspT104I TTCGAA 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 219
BsrI ACTGG 1 cut(s) 206
BssECI CCNNGG 2 cut(s) 690, 912
BssMI GATC 2 cut(s) 40, 58
Bst2UI CCWGG 1 cut(s) 258
Bst4CI ACNGT 2 cut(s) 478, 802
BstBI TTCGAA 1 cut(s) 28
BstC8I GCNNGC 1 cut(s) 281
BstDEI CTNAG 3 cut(s) 402, 534, 843
BstDSI CCRYGG 1 cut(s) 690
BstF5I GGATG 6 cut(s) 280, 451, 526, 550, 629, 904
BstKTI GATC 2 cut(s) 43, 61
BstMBI GATC 2 cut(s) 40, 58
BstMWI GCNNNNNNNGC 1 cut(s) 147
BstNI CCWGG 1 cut(s) 258
BstPAI GACNNNNGTC 1 cut(s) 411
BstSCI CCNGG 3 cut(s) 168, 256, 912
BstSLI GKGCMC 1 cut(s) 465
BstV2I GAAGAC 1 cut(s) 393
BstX2I RGATCY 2 cut(s) 40, 58
BstYI RGATCY 2 cut(s) 40, 58
Bsu36I CCTNAGG 1 cut(s) 534
BsuI GTATCC 1 cut(s) 186
BsuRI GGCC 6 cut(s) 141, 206, 463, 505, 532, 613
BtgI CCRYGG 1 cut(s) 690
BtsCI GGATG 6 cut(s) 280, 451, 526, 550, 629, 904
BtsIMutI CAGTG 1 cut(s) 798
Cac8I GCNNGC 1 cut(s) 281
CaiI CAGNNNCTG 1 cut(s) 842
Cfr13I GGNCC 9 cut(s) 139, 204, 353, 461, 462, 503, 530, 554, 611
Csp6I GTAC 3 cut(s) 220, 362, 796
CviAII CATG 9 cut(s) 89, 184, 311, 416, 494, 527, 602, 608, 634
CviQI GTAC 3 cut(s) 220, 362, 796
DdeI CTNAG 3 cut(s) 402, 534, 843
DpnI GATC 2 cut(s) 42, 60
DpnII GATC 2 cut(s) 40, 58
DriI GACNNNNNGTC 1 cut(s) 178
Eam1105I GACNNNNNGTC 1 cut(s) 178
Eco24I GRGCYC 1 cut(s) 465
Eco47I GGWCC 2 cut(s) 353, 554
Eco81I CCTNAGG 1 cut(s) 534
EcoO109I RGGNCCY 2 cut(s) 353, 554
EcoRI GAATTC 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 256
EcoT38I GRGCYC 1 cut(s) 465
FaeI CATG 9 cut(s) 92, 187, 314, 419, 497, 530, 605, 611, 637
FaqI GGGAC 1 cut(s) 129
FatI CATG 9 cut(s) 88, 183, 310, 415, 493, 526, 601, 607, 633
FauNDI CATATG 1 cut(s) 514
FblI GTMKAC 1 cut(s) 165
FokI GGATG 6 cut(s) 287, 458, 533, 557, 616, 911
FriOI GRGCYC 1 cut(s) 465
FspBI CTAG 1 cut(s) 768
GsuI CTGGAG 1 cut(s) 795
HaeIII GGCC 6 cut(s) 141, 206, 463, 505, 532, 613
HapII CCGG 2 cut(s) 170, 914
Hin1II CATG 9 cut(s) 92, 187, 314, 419, 497, 530, 605, 611, 637
HinfI GANTC 5 cut(s) 82, 389, 839, 848, 872
HpaII CCGG 2 cut(s) 170, 914
HphI GGTGA 1 cut(s) 596
Hpy166II GTNNAC 3 cut(s) 166, 439, 798
Hpy188I TCNGA 6 cut(s) 306, 370, 826, 844, 853, 877
Hpy188III TCNNGA 3 cut(s) 536, 774, 806
Hpy8I GTNNAC 3 cut(s) 166, 439, 798
HpyAV CCTTC 3 cut(s) 344, 720, 736
HpyCH4III ACNGT 2 cut(s) 478, 802
HpyCH4V TGCA 3 cut(s) 548, 716, 751
HpyF10VI GCNNNNNNNGC 1 cut(s) 147
HpyF3I CTNAG 3 cut(s) 402, 534, 843
Hsp92II CATG 9 cut(s) 92, 187, 314, 419, 497, 530, 605, 611, 637
KpnI GGTACC 1 cut(s) 223
Kzo9I GATC 2 cut(s) 40, 58
LmnI GCTCC 1 cut(s) 824
LweI GCATC 3 cut(s) 472, 535, 638
MaeI CTAG 1 cut(s) 768
MaeIII GTNAC 1 cut(s) 905
MalI GATC 2 cut(s) 42, 60
MboI GATC 2 cut(s) 40, 58
MboII GAAGA 9 cut(s) 308, 395, 398, 599, 602, 681, 710, 713, 733
MfeI CAATTG 1 cut(s) 143
MflI RGATCY 2 cut(s) 40, 58
MhlI GDGCHC 1 cut(s) 465
MluCI AATT 5 cut(s) 30, 143, 234, 239, 252
MlyI GAGTC 2 cut(s) 383, 842
MmeI TCCRAC 1 cut(s) 553
MseI TTAA 1 cut(s) 887
MslI CAYNNNNRTG 2 cut(s) 498, 606
MspI CCGG 2 cut(s) 170, 914
MspR9I CCNGG 3 cut(s) 170, 258, 914
MunI CAATTG 1 cut(s) 143
MvaI CCWGG 1 cut(s) 258
MwoI GCNNNNNNNGC 1 cut(s) 147
NciI CCSGG 2 cut(s) 170, 914
NdeI CATATG 1 cut(s) 514
NdeII GATC 2 cut(s) 40, 58
NlaIII CATG 9 cut(s) 92, 187, 314, 419, 497, 530, 605, 611, 637
NlaIV GGNNCC 5 cut(s) 42, 140, 221, 355, 463
NmuCI GTSAC 1 cut(s) 905
NspV TTCGAA 1 cut(s) 28
PfeI GAWTC 3 cut(s) 82, 839, 872
PfoI TCCNGGA 1 cut(s) 256
PleI GAGTC 2 cut(s) 383, 842
PpsI GAGTC 2 cut(s) 383, 842
PpuMI RGGWCCY 2 cut(s) 353, 554
PshAI GACNNNNGTC 1 cut(s) 411
Psp5II RGGWCCY 2 cut(s) 353, 554
Psp6I CCWGG 1 cut(s) 256
PspGI CCWGG 1 cut(s) 256
PspN4I GGNNCC 5 cut(s) 42, 140, 221, 355, 463
PspOMI GGGCCC 1 cut(s) 461
PspPI GGNCC 9 cut(s) 139, 204, 353, 461, 462, 503, 530, 554, 611
PspPPI RGGWCCY 2 cut(s) 353, 554
PstNI CAGNNNCTG 1 cut(s) 842
PsuI RGATCY 2 cut(s) 40, 58
RsaI GTAC 3 cut(s) 221, 363, 797
RsaNI GTAC 3 cut(s) 220, 362, 796
RseI CAYNNNNRTG 2 cut(s) 498, 606
SaqAI TTAA 1 cut(s) 887
Sau3AI GATC 2 cut(s) 40, 58
Sau96I GGNCC 9 cut(s) 139, 204, 353, 461, 462, 503, 530, 554, 611
SchI GAGTC 2 cut(s) 383, 842
ScrFI CCNGG 3 cut(s) 170, 258, 914
SduI GDGCHC 1 cut(s) 465
SfaNI GCATC 3 cut(s) 472, 535, 638
SfuI TTCGAA 1 cut(s) 28
SinI GGWCC 2 cut(s) 353, 554
SmiMI CAYNNNNRTG 2 cut(s) 498, 606
Sse9I AATT 5 cut(s) 30, 143, 234, 239, 252
SsiI CCGC 1 cut(s) 47
SspMI CTAG 1 cut(s) 768
StyD4I CCNGG 3 cut(s) 168, 256, 912
TaaI ACNGT 2 cut(s) 478, 802
TaqI TCGA 1 cut(s) 28
TasI AATT 5 cut(s) 30, 143, 234, 239, 252
TfiI GAWTC 3 cut(s) 82, 839, 872
Tru1I TTAA 1 cut(s) 887
Tru9I TTAA 1 cut(s) 887
TscAI CASTG 1 cut(s) 805
TseFI GTSAC 1 cut(s) 905
Tsp45I GTSAC 1 cut(s) 905
TspDTI ATGAA 5 cut(s) 74, 92, 310, 576, 650
TspRI CASTG 1 cut(s) 805
VpaK11BI GGWCC 2 cut(s) 353, 554
XapI RAATTY 1 cut(s) 252
XcmI CCANNNNNNNNNTGG 1 cut(s) 240
XmiI GTMKAC 1 cut(s) 165
XspI CTAG 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.