Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0007446

Physical Location & Seq

Genomic Coordinates Reverse (-)

77258525 .. 77262599

Gene Structure 4075 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G043960.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGGCTCTGTCTTCACTGCGACTCCCGGTCTCGCTTTGGCTTCCAAAACATCCAAACCAGAGCTTCCATTTTTCAAACCCAAAAATACCCTCAAGATTGAACCTTATTTACTGCTGCTCCCCCTCCGCTGCCACTGCTCAACAAACCTTAACCCGCACTGAAAAATGGCAACCCGTTCGAAAGAAGAAAGTGGTTCTCCGGGTCGGGTATGTGGGCACTGATTACCGAGGTCTGCAAATGCAAAGAGATGACCCTTCAATATCTACTATTGAAAAGGAACTGGAAACTGCCATATTCAAGGCCGGTGGTATTCGCGAGAGTAATTTCGGGAACCTGAACAAGATTGCGTGGGCCAGAAGCAGCAGAACCGATAAGGGAGTTCACTCCTTGGCGACAATGATATCATTGAAAATGGAAATCCCTGAAAATGCGTGGGACGGAGACCTGTATGGCATTGCTCTTGTGAATCACATCAATTGTTACCTTCCTCATAATATCAAAATTTTTAGTGTTTTACCTGCACAGAGGAGCTTTGATCCCAGAAAGGAGTGTAATCTCCGTATGTATTCATACCTCCTTCCTGCTGATGTTATTGGAATCAAAAGTCACTTTAGCACAGCTGAAATTGATGATCACATTGCAGACTTCAACGATATACTAAATGCTTTCGAGGGTGAACATCCTTTTCATAACTATACAGTACGATCTAAATACAGGAAAAAACTTTCTGGGAAGAAATCACCTATATACCGCAATATGTCAAAAACAGAAAGATCCACTGTTGAGGCATCAGCCTCCGAGCTTGAGGGAAGTGACGATGAAGATGAGTTCGAGGGAGAAAATACAACAGAAACTGAGGTACTGAACCAAAATTCTTTCCTGTCCAGCAATGGGCATGAAAACTTACTGAAAGCATGCAAGAAGCAGGAAGATGTTCTGAAGGAGAAAAGTACTGATTCAGTTATCCGTGCAAAATGGTTATACGAACCTGATGAGAGTGATAGAATCGGTGCTTCTCATTTCAGAAAGATATTTCGCTGTTCTTGTGGAAAGCTTGAAAAATCGCTGGCACATGAGTATGTTGAGCTCTCCATATGGGGAGAGTCTTTCATGTTGCATCAAATTCGCAAAATGGTTGGAACTGCTGTGGCCATAAAGCGCAAGTTACTCCCAAGAGATATTCTGACATTATCACTGGCCAAGTTCTCACGGATAATCCTACCCCTTGCTCCATCTGAAGTTTTGATCTTAAGAGGAAACAGATTTTCAATTAGAGAAAGACCTGGAAATGTAACAAGGCCTGAAATGATTGCAATGGTTGAATCAGAAGAAATTATCAAACAGGTTGATGAGTTCTTCACATCTGTAATGTTGCCTCAAGTTGCAAAGTTCTTAGAACCTTCAGGATATCCTTGGCAAGAATGGATTGAAAACTTGGATAGAAACACAGGTATTCCAGATATAGAATTGGATGAAGTTAGAAATGCTTGGAAGGTGTGGAAGGATAGGTTTGAGAGTGATGCATCAATCCTGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001522 GO:0003674 GO:0003824 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016853 GO:0016866 GO:0031119 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PseudoU_synth_1

Protein Analysis

512

Amino Acids

58.6

Weight (kDa)

8.03

Isoelectric Point (pI)

42.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PseudoU_synth_1	PF01416	338 - 414	1e-05	tRNA pseudouridine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007446)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30320
fragaria_vesca	FvH4_3g40070 FvH4_3g40070 FvH4_3g40070
malus_domestica	MD11G1060800.v1.1
prunus_persica	Prupe.6G045100_v2.0.a1 Prupe.6G045100_v2.0.a1 Prupe.6G045100_v2.0.a1 Prupe.6G045100_v2.0.a1 Prupe.6G045100_v2.0.a1 Prupe.6G045100_v2.0.a1 Prupe.6G045100_v2.0.a1
pyrus_communis	pycom11g05030
rosa_chinensis	RchiOBHm_Chr5g0072261
rosa_laevigata	RLG00000036289
rosa_multiflora	Rmu_sc0000714.1_g000004
rosa_roxburghii	Rroxscaffold_1G00009010
rosa_rugosa	Rorug05G0417100 Rorug05G0417200 Rorug05G0417300
rosa_samantha	Rh5AG473100 Rh5BG493300 Rh5CG517300 Rh5DG505300
rosa_wichuraiana	Rw5G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	526
AccII	CGCG	1 cut(s)	315
AciI	CCGC	3 cut(s)	126, 154, 751
AclWI	GGATC	2 cut(s)	530, 768
AcoI	YGGCCR	2 cut(s)	1149, 1197
AcsI	RAATTY	3 cut(s)	501, 871, 1122
AcuI	CTGAAG	3 cut(s)	959, 1257, 1386
AfaI	GTAC	3 cut(s)	702, 861, 952
AfiI	CCNNNNNNNGG	1 cut(s)	891
AflII	CTTAAG	1 cut(s)	1249
AhdI	GACNNNNNGTC	1 cut(s)	26
AjnI	CCWGG	1 cut(s)	1282
AluBI	AGCT	6 cut(s)	63, 531, 620, 802, 1054, 1087
AluI	AGCT	6 cut(s)	63, 531, 620, 802, 1054, 1087
Alw21I	GWGCWC	1 cut(s)	1089
Alw26I	GTCTC	2 cut(s)	34, 435
AlwI	GGATC	2 cut(s)	530, 768
AlwNI	CAGNNNCTG	1 cut(s)	854
AoxI	GGCC	5 cut(s)	300, 351, 1149, 1197, 1298
ApeKI	GCWGC	3 cut(s)	114, 128, 360
ApoI	RAATTY	3 cut(s)	501, 871, 1122
Asp700I	GAANNNNTTC	1 cut(s)	933
AspLEI	GCGC	1 cut(s)	1161
AspS9I	GGNCC	1 cut(s)	351
AsuC2I	CCSGG	2 cut(s)	26, 200
AsuHPI	GGTGA	2 cut(s)	686, 732
AsuII	TTCGAA	1 cut(s)	178
BaeGI	GKGCMC	1 cut(s)	218
BalI	TGGCCA	2 cut(s)	1151, 1199
BanII	GRGCYC	1 cut(s)	1089
BbsI	GAAGAC	1 cut(s)	3
Bbv12I	GWGCWC	1 cut(s)	1089
BbvI	GCAGC	3 cut(s)	101, 115, 372
BccI	CCATC	1 cut(s)	1240
BcgI	CGANNNNNNTGC	4 cut(s)	158, 192, 1106, 1140
BciT130I	CCWGG	1 cut(s)	1284
BclI	TGATCA	1 cut(s)	631
BcnI	CCSGG	2 cut(s)	26, 200
BcoDI	GTCTC	2 cut(s)	34, 435
BfrI	CTTAAG	1 cut(s)	1249
BfuAI	ACCTGC	1 cut(s)	526
BisI	GCNGC	3 cut(s)	115, 129, 361
BlsI	GCNGC	3 cut(s)	116, 130, 362
BmcAI	AGTACT	1 cut(s)	952
Bme1390I	CCNGG	3 cut(s)	26, 200, 1284
BmeRI	GACNNNNNGTC	1 cut(s)	26
BmgT120I	GGNCC	1 cut(s)	351
BmiI	GGNNCC	1 cut(s)	332
BmrFI	CCNGG	3 cut(s)	26, 200, 1284
BmsI	GCATC	4 cut(s)	797, 1126, 1510, 1532
BpiI	GAAGAC	1 cut(s)	3
Bpu14I	TTCGAA	1 cut(s)	178
BpuEI	CTTGAG	3 cut(s)	76, 824, 1362
BpuMI	CCSGG	2 cut(s)	26, 200
BsaI	GGTCTC	2 cut(s)	34, 435
BsaJI	CCNNGG	3 cut(s)	226, 387, 1412
BsaXI	ACNNNNNCTCC	4 cut(s)	6, 36, 101, 131
Bsc4I	CCNNNNNNNGG	1 cut(s)	891
Bse118I	RCCGGY	1 cut(s)	302
Bse1I	ACTGG	2 cut(s)	285, 1200
Bse3DI	GCAATG	4 cut(s)	453, 636, 895, 1320
BseBI	CCWGG	1 cut(s)	1284
BseDI	CCNNGG	3 cut(s)	226, 387, 1412
BseGI	GGATG	3 cut(s)	49, 679, 1477
BseLI	CCNNNNNNNGG	1 cut(s)	891
BseMI	GCAATG	4 cut(s)	453, 636, 895, 1320
BseMII	CTCAG	1 cut(s)	846
BseNI	ACTGG	2 cut(s)	285, 1200
BseRI	GAGGAG	1 cut(s)	541
BseSI	GKGCMC	1 cut(s)	218
BseXI	GCAGC	3 cut(s)	101, 115, 372
BsgI	GTGCAG	1 cut(s)	504
Bsh1236I	CGCG	1 cut(s)	315
BshFI	GGCC	5 cut(s)	302, 353, 1151, 1199, 1300
BsiHKAI	GWGCWC	1 cut(s)	1089
BsiSI	CCGG	3 cut(s)	26, 199, 303
BslFI	GGGAC	1 cut(s)	449
BslI	CCNNNNNNNGG	1 cut(s)	891
BsmAI	GTCTC	2 cut(s)	34, 435
BsmFI	GGGAC	1 cut(s)	449
BsnI	GGCC	5 cut(s)	302, 353, 1151, 1199, 1300
Bso31I	GGTCTC	2 cut(s)	34, 435
Bsp119I	TTCGAA	1 cut(s)	178
Bsp1286I	GDGCHC	2 cut(s)	218, 1089
Bsp143I	GATC	5 cut(s)	535, 631, 704, 773, 1245
Bsp68I	TCGCGA	1 cut(s)	315
BspACI	CCGC	3 cut(s)	126, 154, 751
BspANI	GGCC	5 cut(s)	302, 353, 1151, 1199, 1300
BspCNI	CTCAG	1 cut(s)	847
BspFNI	CGCG	1 cut(s)	315
BspLI	GGNNCC	1 cut(s)	332
BspMI	ACCTGC	1 cut(s)	526
BspPI	GGATC	2 cut(s)	530, 768
BspT104I	TTCGAA	1 cut(s)	178
BspTI	CTTAAG	1 cut(s)	1249
BspTNI	GGTCTC	2 cut(s)	34, 435
BsrDI	GCAATG	4 cut(s)	453, 636, 895, 1320
BsrFI	RCCGGY	1 cut(s)	302
BsrI	ACTGG	2 cut(s)	285, 1200
BssAI	RCCGGY	1 cut(s)	302
BssECI	CCNNGG	3 cut(s)	226, 387, 1412
BssMI	GATC	5 cut(s)	535, 631, 704, 773, 1245
BssT1I	CCWWGG	2 cut(s)	387, 1412
Bst2UI	CCWGG	1 cut(s)	1284
Bst4CI	ACNGT	2 cut(s)	700, 781
BstAFI	CTTAAG	1 cut(s)	1249
BstBI	TTCGAA	1 cut(s)	178
BstC8I	GCNNGC	2 cut(s)	916, 1068
BstDEI	CTNAG	2 cut(s)	855, 1393
BstF5I	GGATG	3 cut(s)	49, 679, 1477
BstFNI	CGCG	1 cut(s)	315
BstHHI	GCGC	1 cut(s)	1161
BstKTI	GATC	5 cut(s)	538, 634, 707, 776, 1248
BstMAI	GTCTC	2 cut(s)	34, 435
BstMBI	GATC	5 cut(s)	535, 631, 704, 773, 1245
BstMWI	GCNNNNNNNGC	1 cut(s)	134
BstNI	CCWGG	1 cut(s)	1284
BstNSI	RCATGY	1 cut(s)	918
BstSCI	CCNGG	3 cut(s)	24, 198, 1282
BstSLI	GKGCMC	1 cut(s)	218
BstUI	CGCG	1 cut(s)	315
BstV1I	GCAGC	3 cut(s)	101, 115, 372
BstV2I	GAAGAC	1 cut(s)	3
BstX2I	RGATCY	1 cut(s)	773
BstYI	RGATCY	1 cut(s)	773
BsuRI	GGCC	5 cut(s)	302, 353, 1151, 1199, 1300
BtsCI	GGATG	3 cut(s)	49, 679, 1477
BtsI	GCAGTG	2 cut(s)	14, 132
BtsIMutI	CAGTG	6 cut(s)	14, 132, 156, 216, 777, 1193
BtuMI	TCGCGA	1 cut(s)	315
BveI	ACCTGC	1 cut(s)	526
Cac8I	GCNNGC	2 cut(s)	916, 1068
CaiI	CAGNNNCTG	1 cut(s)	854
CfoI	GCGC	1 cut(s)	1161
Cfr10I	RCCGGY	1 cut(s)	302
Cfr13I	GGNCC	1 cut(s)	351
Csp6I	GTAC	3 cut(s)	701, 860, 951
CspCI	CAANNNNNGTGG	2 cut(s)	286, 321
CviAII	CATG	4 cut(s)	896, 915, 1073, 1111
CviQI	GTAC	3 cut(s)	701, 860, 951
DdeI	CTNAG	2 cut(s)	855, 1393
DpnI	GATC	5 cut(s)	537, 633, 706, 775, 1247
DpnII	GATC	5 cut(s)	535, 631, 704, 773, 1245
DriI	GACNNNNNGTC	1 cut(s)	26
EaeI	YGGCCR	2 cut(s)	1149, 1197
Eam1105I	GACNNNNNGTC	1 cut(s)	26
Ecl136II	GAGCTC	1 cut(s)	1087
Eco130I	CCWWGG	2 cut(s)	387, 1412
Eco147I	AGGCCT	1 cut(s)	1300
Eco24I	GRGCYC	1 cut(s)	1089
Eco31I	GGTCTC	2 cut(s)	34, 435
Eco32I	GATATC	2 cut(s)	402, 1409
Eco53kI	GAGCTC	1 cut(s)	1087
Eco57I	CTGAAG	3 cut(s)	959, 1257, 1386
EcoICRI	GAGCTC	1 cut(s)	1087
EcoRII	CCWGG	1 cut(s)	1282
EcoRV	GATATC	2 cut(s)	402, 1409
EcoT14I	CCWWGG	2 cut(s)	387, 1412
EcoT22I	ATGCAT	1 cut(s)	1525
EcoT38I	GRGCYC	1 cut(s)	1089
ErhI	CCWWGG	2 cut(s)	387, 1412
FaeI	CATG	4 cut(s)	899, 918, 1076, 1114
FaqI	GGGAC	1 cut(s)	449
FatI	CATG	4 cut(s)	895, 914, 1072, 1110
FauI	CCCGC	1 cut(s)	161
FauNDI	CATATG	1 cut(s)	1094
FbaI	TGATCA	1 cut(s)	631
Fnu4HI	GCNGC	3 cut(s)	115, 129, 361
FokI	GGATG	3 cut(s)	36, 666, 1484
FriOI	GRGCYC	1 cut(s)	1089
Fsp4HI	GCNGC	3 cut(s)	115, 129, 361
GlaI	GCGC	1 cut(s)	1160
GluI	GCNGC	3 cut(s)	115, 129, 361
HaeIII	GGCC	5 cut(s)	302, 353, 1151, 1199, 1300
HapII	CCGG	3 cut(s)	26, 199, 303
HhaI	GCGC	1 cut(s)	1161
Hin1II	CATG	4 cut(s)	899, 918, 1076, 1114
Hin6I	GCGC	1 cut(s)	1159
HinP1I	GCGC	1 cut(s)	1159
HindIII	AAGCTT	1 cut(s)	1052
HinfI	GANTC	7 cut(s)	21, 466, 597, 956, 1005, 1103, 1322
HpaII	CCGG	3 cut(s)	26, 199, 303
HphI	GGTGA	2 cut(s)	686, 732
Hpy166II	GTNNAC	2 cut(s)	382, 677
Hpy188I	TCNGA	6 cut(s)	799, 939, 1025, 1185, 1237, 1327
Hpy188III	TCNNGA	6 cut(s)	93, 314, 328, 1404, 1457, 1531
Hpy8I	GTNNAC	2 cut(s)	382, 677
HpyAV	CCTTC	7 cut(s)	264, 494, 587, 934, 1410, 1486, 1495
HpyCH4III	ACNGT	2 cut(s)	700, 781
HpyF10VI	GCNNNNNNNGC	1 cut(s)	134
HpyF3I	CTNAG	2 cut(s)	855, 1393
Hsp92II	CATG	4 cut(s)	899, 918, 1076, 1114
HspAI	GCGC	1 cut(s)	1159
Ksp22I	TGATCA	1 cut(s)	631
Kzo9I	GATC	5 cut(s)	535, 631, 704, 773, 1245
LmnI	GCTCC	3 cut(s)	122, 528, 1234
Lsp1109I	GCAGC	3 cut(s)	101, 115, 372
LweI	GCATC	4 cut(s)	797, 1126, 1510, 1532
MaeIII	GTNAC	5 cut(s)	479, 605, 812, 1164, 1291
MalI	GATC	5 cut(s)	537, 633, 706, 775, 1247
MboI	GATC	5 cut(s)	535, 631, 704, 773, 1245
MboII	GAAGA	7 cut(s)	3, 196, 745, 833, 941, 1340, 1348
MfeI	CAATTG	1 cut(s)	475
MflI	RGATCY	1 cut(s)	773
MhlI	GDGCHC	2 cut(s)	218, 1089
MlsI	TGGCCA	2 cut(s)	1151, 1199
MluCI	AATT	9 cut(s)	322, 475, 501, 624, 871, 1122, 1269, 1332, 1466
MluNI	TGGCCA	2 cut(s)	1151, 1199
MlyI	GAGTC	2 cut(s)	15, 1112
MmeI	TCCRAC	1 cut(s)	1117
Mox20I	TGGCCA	2 cut(s)	1151, 1199
Mph1103I	ATGCAT	1 cut(s)	1525
MroXI	GAANNNNTTC	1 cut(s)	933
MscI	TGGCCA	2 cut(s)	1151, 1199
MseI	TTAA	2 cut(s)	149, 1250
MslI	CAYNNNNRTG	1 cut(s)	1077
Msp20I	TGGCCA	2 cut(s)	1151, 1199
MspA1I	CMGCKG	2 cut(s)	128, 620
MspCI	CTTAAG	1 cut(s)	1249
MspI	CCGG	3 cut(s)	26, 199, 303
MspR9I	CCNGG	3 cut(s)	26, 200, 1284
MunI	CAATTG	1 cut(s)	475
MvaI	CCWGG	1 cut(s)	1284
MvnI	CGCG	1 cut(s)	315
MwoI	GCNNNNNNNGC	1 cut(s)	134
NciI	CCSGG	2 cut(s)	26, 200
NdeI	CATATG	1 cut(s)	1094
NdeII	GATC	5 cut(s)	535, 631, 704, 773, 1245
NlaIII	CATG	4 cut(s)	899, 918, 1076, 1114
NlaIV	GGNNCC	1 cut(s)	332
NmuCI	GTSAC	2 cut(s)	605, 812
NruI	TCGCGA	1 cut(s)	315
NsiI	ATGCAT	1 cut(s)	1525
NspI	RCATGY	1 cut(s)	918
NspV	TTCGAA	1 cut(s)	178
PaeI	GCATGC	1 cut(s)	918
PceI	AGGCCT	1 cut(s)	1300
PdmI	GAANNNNTTC	1 cut(s)	933
PfeI	GAWTC	5 cut(s)	466, 597, 956, 1005, 1322
PkrI	GCNGC	3 cut(s)	116, 130, 362
PleI	GAGTC	2 cut(s)	15, 1111
PpsI	GAGTC	2 cut(s)	15, 1111
Psp124BI	GAGCTC	1 cut(s)	1089
Psp6I	CCWGG	1 cut(s)	1282
PspGI	CCWGG	1 cut(s)	1282
PspN4I	GGNNCC	1 cut(s)	332
PspPI	GGNCC	1 cut(s)	351
PsrI	GAACNNNNNNTAC	2 cut(s)	92, 124
PstNI	CAGNNNCTG	1 cut(s)	854
PsuI	RGATCY	1 cut(s)	773
PvuII	CAGCTG	1 cut(s)	620
RruI	TCGCGA	1 cut(s)	315
RsaI	GTAC	3 cut(s)	702, 861, 952
RsaNI	GTAC	3 cut(s)	701, 860, 951
RseI	CAYNNNNRTG	1 cut(s)	1077
SacI	GAGCTC	1 cut(s)	1089
SaqAI	TTAA	2 cut(s)	149, 1250
SatI	GCNGC	3 cut(s)	115, 129, 361
Sau3AI	GATC	5 cut(s)	535, 631, 704, 773, 1245
Sau96I	GGNCC	1 cut(s)	351
ScaI	AGTACT	1 cut(s)	952
SchI	GAGTC	2 cut(s)	15, 1112
ScrFI	CCNGG	3 cut(s)	26, 200, 1284
SduI	GDGCHC	2 cut(s)	218, 1089
SfaNI	GCATC	4 cut(s)	797, 1126, 1510, 1532
SfuI	TTCGAA	1 cut(s)	178
SmiMI	CAYNNNNRTG	1 cut(s)	1077
SmlI	CTYRAG	4 cut(s)	91, 803, 1249, 1377
SmoI	CTYRAG	4 cut(s)	91, 803, 1249, 1377
SphI	GCATGC	1 cut(s)	918
Sse9I	AATT	9 cut(s)	322, 475, 501, 624, 871, 1122, 1269, 1332, 1466
SseBI	AGGCCT	1 cut(s)	1300
SsiI	CCGC	3 cut(s)	126, 154, 751
SstI	GAGCTC	1 cut(s)	1089
StuI	AGGCCT	1 cut(s)	1300
StyD4I	CCNGG	3 cut(s)	24, 198, 1282
StyI	CCWWGG	2 cut(s)	387, 1412
TaaI	ACNGT	2 cut(s)	700, 781
TaqI	TCGA	3 cut(s)	178, 669, 831
TasI	AATT	9 cut(s)	322, 475, 501, 624, 871, 1122, 1269, 1332, 1466
TatI	WGTACW	1 cut(s)	950
TfiI	GAWTC	5 cut(s)	466, 597, 956, 1005, 1322
Tru1I	TTAA	2 cut(s)	149, 1250
Tru9I	TTAA	2 cut(s)	149, 1250
TscAI	CASTG	6 cut(s)	21, 139, 163, 223, 784, 1200
TseFI	GTSAC	2 cut(s)	605, 812
TseI	GCWGC	3 cut(s)	114, 128, 360
Tsp45I	GTSAC	2 cut(s)	605, 812
TspDTI	ATGAA	6 cut(s)	558, 677, 834, 912, 1099, 1488
TspGWI	ACGGA	4 cut(s)	453, 548, 956, 1225
TspRI	CASTG	6 cut(s)	21, 139, 163, 223, 784, 1200
Vha464I	CTTAAG	1 cut(s)	1249
XapI	RAATTY	3 cut(s)	501, 871, 1122
XceI	RCATGY	1 cut(s)	918
XmnI	GAANNNNTTC	1 cut(s)	933
ZrmI	AGTACT	1 cut(s)	952
Zsp2I	ATGCAT	1 cut(s)	1525

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.