Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_co8474845.1

Orthogroup ID

OG0008397

Physical Location & Seq

Genomic Coordinates Reverse (-)

124 .. 1586

Gene Structure 1463 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_co8474845.1_g000001.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1215 bp

atggttgttgatggtggtgctcaatggggtccacgaggtgctcgtccatcccagcactcttcatatgattatcagcaacgaggaccatatcagtcccgtactcagtaccctcctgcatatgggaattatcctcaacatatgggtcctagaagtggctttggttctggttgggagcaaaggccacctccaaacatgcaggggatgcctccacatggcggtggttatgattactatggcagacaaggagctgaagccccagcccaacattctactcatggcccttctcatgctcctggcccttctcctaaccctataatgggtggacctccatcccaagcaaactataattatggacagccacatggttcagactataggcatccggctcctttttctcatgctgcaccccctcagcatggctatgggcatgggtatgaagaaccaaaatatgataatcatgctccaccacagcactcatatggaggacatggagcttctcagccgtatccacaaactggagcccagccaggttatggtccacagcagcattatggtaagccaccagcatataccatgccatctcagggaccacctccccagtcttatggcgcccccatgggtagccaaccaggagaagcggtttatcagacaccagctcagtcatatggtcagaatgtcccagctcaacagccatatccatatgcatccactgcacctgcacagcagacttatgctccgtatggttctgccccagctgctgatgggtacactcagccaccaactgcttcgagcgcaggttatccacagcaaggagggcagcctgctagttatggtcagcctggctcgcagcctgcacctggctatgcacaagcagctcctgcagggtatgcacaatacccaacgtctcaacaaggttactccgagcagtctgcttcatacactggtggttatgggtaccaagcgtctcaggatcctgcctatggaggtgtctctgcacctgcctatggtggagctccagctgcgcaacagggctacgcacaaccagcacccgcagcagctggggcacagcagggctacacccagccagcaccagcaacaactcaacaaagctatgatcaatcaatcccgcagtcaggcggttatgggaaaacagtgtcacctcagcctggctacgctcaatacgactcgagtcaaatgtatgctgcacctcgttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1

Protein Analysis

404

Amino Acids

42.17

Weight (kDa)

6.55

Isoelectric Point (pI)

71.8

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008397)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33680 AT4G10070
fragaria_vesca	FvH4_2g35080 FvH4_2g35080 FvH4_2g35080
malus_domestica	MD08G1108100.v1.1 MD15G1088600.v1.1 MD15G1088800.v1.1
prunus_persica	Prupe.1G442700_v2.0.a1
pyrus_communis	pycom08g09010 pycom15g08310
rosa_chinensis	RchiOBHm_Chr6g0306891
rosa_laevigata	RLG00000010757
rosa_multiflora	Rmu_co8448611.1_g000001 Rmu_co8474845.1_g000001 Rmu_sc0018770.1_g000003
rosa_roxburghii	Rroxscaffold_7G00161380
rosa_rugosa	Rorug06G0354100
rosa_samantha	Rh6AG465700 Rh6BG435100 Rh6CG480200 Rh6DG467100
rosa_wichuraiana	Rw6G040590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	724, 1006
Acc16I	TGCGCA	1 cut(s)	1023
Acc36I	ACCTGC	3 cut(s)	724, 785, 1006
Acc65I	GGTACC	1 cut(s)	954
AccB1I	GGYRCC	2 cut(s)	608, 954
AccB7I	CCANNNNNTGG	2 cut(s)	515, 533
AciI	CCGC	5 cut(s)	216, 638, 1050, 1127, 1137
AclWI	GGATC	2 cut(s)	965, 978
AcuI	CTGAAG	1 cut(s)	270
AcyI	GRCGYC	1 cut(s)	609
AdeI	CACNNNGTG	1 cut(s)	38
AfaI	GTAC	4 cut(s)	100, 107, 767, 956
AjnI	CCWGG	6 cut(s)	292, 526, 628, 838, 856, 1165
Alw21I	GWGCWC	3 cut(s)	22, 43, 1015
Alw26I	GTCTC	3 cut(s)	909, 969, 994
AlwI	GGATC	2 cut(s)	965, 978
AlwNI	CAGNNNCTG	3 cut(s)	758, 878, 1058
Ama87I	CYCGRG	1 cut(s)	1186
AoxI	GGCC	3 cut(s)	179, 277, 295
Asp718I	GGTACC	1 cut(s)	954
AspLEI	GCGC	3 cut(s)	611, 794, 1024
AspS9I	GGNCC	8 cut(s)	29, 83, 143, 278, 296, 323, 536, 587
AsuHPI	GGTGA	1 cut(s)	1149
AvaI	CYCGRG	1 cut(s)	1186
AvaII	GGWCC	6 cut(s)	29, 83, 143, 323, 536, 587
BaeGI	GKGCMC	1 cut(s)	1066
BamHI	GGATCC	1 cut(s)	970
BanI	GGYRCC	2 cut(s)	608, 954
BanII	GRGCYC	2 cut(s)	523, 1015
BauI	CACGAG	1 cut(s)	33
Bbv12I	GWGCWC	3 cut(s)	22, 43, 1015
BbvCI	CCTCAGC	2 cut(s)	411, 1161
BccI	CCATC	5 cut(s)	5, 55, 337, 586, 755
BceAI	ACGGC	1 cut(s)	487
BcgI	CGANNNNNNTGC	2 cut(s)	911, 945
BciT130I	CCWGG	6 cut(s)	294, 528, 630, 840, 858, 1167
BciVI	GTATCC	1 cut(s)	516
BclI	TGATCA	1 cut(s)	1114
BcoDI	GTCTC	3 cut(s)	909, 969, 994
BfaI	CTAG	2 cut(s)	147, 825
BfmI	CTRYAG	2 cut(s)	373, 879
BfoI	RGCGCY	1 cut(s)	612
BfuAI	ACCTGC	3 cut(s)	724, 785, 1006
BfuI	GTATCC	1 cut(s)	516
Bme1390I	CCNGG	6 cut(s)	294, 528, 630, 840, 858, 1167
Bme18I	GGWCC	6 cut(s)	29, 83, 143, 323, 536, 587
BmeT110I	CYCGRG	1 cut(s)	1186
BmgT120I	GGNCC	8 cut(s)	29, 83, 143, 278, 296, 323, 536, 587
BmiI	GGNNCC	8 cut(s)	30, 144, 387, 520, 588, 610, 956, 972
BmrFI	CCNGG	6 cut(s)	294, 528, 630, 840, 858, 1167
BmrI	ACTGGG	1 cut(s)	592
BmsI	GCATC	3 cut(s)	192, 388, 713
BmuI	ACTGGG	1 cut(s)	592
BoxI	GACNNNNGTC	1 cut(s)	1188
BpmI	CTGGAG	2 cut(s)	537, 999
Bpu10I	CCTNAGC	2 cut(s)	411, 1161
BsaHI	GRCGYC	1 cut(s)	609
BsaJI	CCNNGG	1 cut(s)	615
BsaXI	ACNNNNNCTCC	4 cut(s)	624, 654, 718, 748
Bse1I	ACTGG	3 cut(s)	520, 598, 946
BseBI	CCWGG	6 cut(s)	294, 528, 630, 840, 858, 1167
BseDI	CCNNGG	1 cut(s)	615
BseGI	GGATG	5 cut(s)	47, 207, 329, 379, 704
BseMII	CTCAG	8 cut(s)	116, 425, 512, 596, 671, 785, 980, 1175
BseNI	ACTGG	3 cut(s)	520, 598, 946
BseSI	GKGCMC	1 cut(s)	1066
BseYI	CCCAGC	7 cut(s)	51, 256, 522, 679, 751, 1058, 1080
BsgI	GTGCAG	6 cut(s)	387, 696, 702, 837, 978, 1188
BshFI	GGCC	3 cut(s)	181, 279, 297
BshNI	GGYRCC	2 cut(s)	608, 954
BsiHKAI	GWGCWC	3 cut(s)	22, 43, 1015
BsiHKCI	CYCGRG	1 cut(s)	1186
BsiSI	CCGG	1 cut(s)	383
BslFI	GGGAC	3 cut(s)	79, 600, 662
BsmAI	GTCTC	3 cut(s)	909, 969, 994
BsmBI	CGTCTC	2 cut(s)	909, 969
BsmFI	GGGAC	3 cut(s)	79, 600, 662
BsnI	GGCC	3 cut(s)	181, 279, 297
BsoBI	CYCGRG	1 cut(s)	1186
Bsp1286I	GDGCHC	5 cut(s)	22, 43, 523, 1015, 1066
Bsp143I	GATC	2 cut(s)	970, 1114
Bsp19I	CCATGG	1 cut(s)	615
BspACI	CCGC	5 cut(s)	216, 638, 1050, 1127, 1137
BspANI	GGCC	3 cut(s)	181, 279, 297
BspCNI	CTCAG	8 cut(s)	115, 424, 511, 595, 670, 784, 979, 1174
BspLI	GGNNCC	8 cut(s)	30, 144, 387, 520, 588, 610, 956, 972
BspMAI	CTGCAG	1 cut(s)	883
BspMI	ACCTGC	3 cut(s)	724, 785, 1006
BspPI	GGATC	2 cut(s)	965, 978
BspT107I	GGYRCC	2 cut(s)	608, 954
BsrI	ACTGG	3 cut(s)	520, 598, 946
BssECI	CCNNGG	1 cut(s)	615
BssMI	GATC	2 cut(s)	970, 1114
BssNI	GRCGYC	1 cut(s)	609
BssSI	CACGAG	1 cut(s)	33
BssT1I	CCWWGG	1 cut(s)	615
Bst2BI	CACGAG	1 cut(s)	33
Bst2UI	CCWGG	6 cut(s)	294, 528, 630, 840, 858, 1167
Bst4CI	ACNGT	1 cut(s)	1153
Bst6I	CTCTTC	1 cut(s)	64
BstACI	GRCGYC	1 cut(s)	609
BstAPI	GCANNNNNTGC	4 cut(s)	202, 710, 878, 887
BstC8I	GCNNGC	4 cut(s)	822, 845, 852, 1086
BstDEI	CTNAG	8 cut(s)	102, 411, 498, 582, 657, 771, 966, 1161
BstDSI	CCRYGG	1 cut(s)	615
BstF5I	GGATG	5 cut(s)	47, 207, 329, 379, 704
BstH2I	RGCGCY	1 cut(s)	612
BstHHI	GCGC	3 cut(s)	611, 794, 1024
BstKTI	GATC	2 cut(s)	973, 1117
BstMAI	GTCTC	3 cut(s)	909, 969, 994
BstMBI	GATC	2 cut(s)	970, 1114
BstNI	CCWGG	6 cut(s)	294, 528, 630, 840, 858, 1167
BstNSI	RCATGY	1 cut(s)	196
BstPAI	GACNNNNGTC	1 cut(s)	1188
BstSCI	CCNGG	6 cut(s)	292, 526, 628, 838, 856, 1165
BstSFI	CTRYAG	2 cut(s)	373, 879
BstSLI	GKGCMC	1 cut(s)	1066
BstX2I	RGATCY	1 cut(s)	970
BstXI	CCANNNNNNTGG	1 cut(s)	605
BstYI	RGATCY	1 cut(s)	970
BsuI	GTATCC	1 cut(s)	516
BsuRI	GGCC	3 cut(s)	181, 279, 297
BtgI	CCRYGG	1 cut(s)	615
BtsCI	GGATG	5 cut(s)	47, 207, 329, 379, 704
BtsI	GCAGTG	1 cut(s)	708
BtsIMutI	CAGTG	3 cut(s)	708, 939, 1158
BveI	ACCTGC	3 cut(s)	724, 785, 1006
Cac8I	GCNNGC	4 cut(s)	822, 845, 852, 1086
CaiI	CAGNNNCTG	3 cut(s)	758, 878, 1058
CfoI	GCGC	3 cut(s)	611, 794, 1024
Cfr13I	GGNCC	8 cut(s)	29, 83, 143, 278, 296, 323, 536, 587
CseI	GACGC	1 cut(s)	951
Csp6I	GTAC	4 cut(s)	99, 106, 766, 955
CviQI	GTAC	4 cut(s)	99, 106, 766, 955
DdeI	CTNAG	8 cut(s)	102, 411, 498, 582, 657, 771, 966, 1161
DinI	GGCGCC	1 cut(s)	610
DpnI	GATC	2 cut(s)	972, 1116
DpnII	GATC	2 cut(s)	970, 1114
DraIII	CACNNNGTG	1 cut(s)	38
Eam1104I	CTCTTC	1 cut(s)	64
EarI	CTCTTC	1 cut(s)	64
Ecl136II	GAGCTC	1 cut(s)	1013
Eco130I	CCWWGG	1 cut(s)	615
Eco24I	GRGCYC	2 cut(s)	523, 1015
Eco47I	GGWCC	6 cut(s)	29, 83, 143, 323, 536, 587
Eco53kI	GAGCTC	1 cut(s)	1013
Eco57I	CTGAAG	1 cut(s)	270
Eco88I	CYCGRG	1 cut(s)	1186
EcoICRI	GAGCTC	1 cut(s)	1013
EcoO109I	RGGNCCY	1 cut(s)	143
EcoRII	CCWGG	6 cut(s)	292, 526, 628, 838, 856, 1165
EcoT14I	CCWWGG	1 cut(s)	615
EcoT22I	ATGCAT	1 cut(s)	706
EcoT38I	GRGCYC	2 cut(s)	523, 1015
EgeI	GGCGCC	1 cut(s)	610
EheI	GGCGCC	1 cut(s)	610
ErhI	CCWWGG	1 cut(s)	615
Esp3I	CGTCTC	2 cut(s)	909, 969
FaqI	GGGAC	3 cut(s)	79, 600, 662
FauI	CCCGC	2 cut(s)	1057, 1134
FauNDI	CATATG	6 cut(s)	64, 118, 138, 478, 664, 700
FbaI	TGATCA	1 cut(s)	1114
FokI	GGATG	5 cut(s)	34, 214, 316, 366, 691
FriOI	GRGCYC	2 cut(s)	523, 1015
FspBI	CTAG	2 cut(s)	147, 825
FspI	TGCGCA	1 cut(s)	1023
GlaI	GCGC	3 cut(s)	610, 793, 1023
GsaI	CCCAGC	7 cut(s)	55, 260, 526, 683, 755, 1062, 1084
GsuI	CTGGAG	2 cut(s)	537, 999
HaeII	RGCGCY	1 cut(s)	612
HaeIII	GGCC	3 cut(s)	181, 279, 297
HapII	CCGG	1 cut(s)	383
HgaI	GACGC	1 cut(s)	951
HhaI	GCGC	3 cut(s)	611, 794, 1024
Hin1I	GRCGYC	1 cut(s)	609
Hin6I	GCGC	3 cut(s)	609, 792, 1022
HinP1I	GCGC	3 cut(s)	609, 792, 1022
HinfI	GANTC	2 cut(s)	1184, 1189
HpaII	CCGG	1 cut(s)	383
HphI	GGTGA	1 cut(s)	1149
Hpy166II	GTNNAC	4 cut(s)	32, 323, 539, 768
Hpy188I	TCNGA	4 cut(s)	370, 648, 672, 922
Hpy188III	TCNNGA	1 cut(s)	968
Hpy8I	GTNNAC	4 cut(s)	32, 323, 539, 768
HpyAV	CCTTC	2 cut(s)	291, 309
HpyCH4III	ACNGT	1 cut(s)	1153
HpyCH4IV	ACGT	1 cut(s)	902
HpyF3I	CTNAG	8 cut(s)	102, 411, 498, 582, 657, 771, 966, 1161
HpySE526I	ACGT	1 cut(s)	902
Hsp92I	GRCGYC	1 cut(s)	609
HspAI	GCGC	3 cut(s)	609, 792, 1022
KasI	GGCGCC	1 cut(s)	608
KpnI	GGTACC	1 cut(s)	958
Ksp22I	TGATCA	1 cut(s)	1114
Kzo9I	GATC	2 cut(s)	970, 1114
LweI	GCATC	3 cut(s)	192, 388, 713
MaeI	CTAG	2 cut(s)	147, 825
MaeII	ACGT	1 cut(s)	902
MaeIII	GTNAC	2 cut(s)	914, 1155
MalI	GATC	2 cut(s)	972, 1116
MboI	GATC	2 cut(s)	970, 1114
MboII	GAAGA	2 cut(s)	51, 449
MflI	RGATCY	1 cut(s)	970
MhlI	GDGCHC	5 cut(s)	22, 43, 523, 1015, 1066
MluCI	AATT	2 cut(s)	124, 346
Mly113I	GGCGCC	1 cut(s)	609
MlyI	GAGTC	2 cut(s)	1178, 1198
Mph1103I	ATGCAT	1 cut(s)	706
MslI	CAYNNNNRTG	4 cut(s)	216, 420, 432, 477
MspA1I	CMGCKG	3 cut(s)	755, 1019, 1058
MspI	CCGG	1 cut(s)	383
MspR9I	CCNGG	6 cut(s)	294, 528, 630, 840, 858, 1167
MvaI	CCWGG	6 cut(s)	294, 528, 630, 840, 858, 1167
NarI	GGCGCC	1 cut(s)	609
NcoI	CCATGG	1 cut(s)	615
NdeI	CATATG	6 cut(s)	64, 118, 138, 478, 664, 700
NdeII	GATC	2 cut(s)	970, 1114
NlaIV	GGNNCC	8 cut(s)	30, 144, 387, 520, 588, 610, 956, 972
NmuCI	GTSAC	1 cut(s)	1155
NsbI	TGCGCA	1 cut(s)	1023
NsiI	ATGCAT	1 cut(s)	706
NspI	RCATGY	1 cut(s)	196
PaeR7I	CTCGAG	1 cut(s)	1186
PaqCI	CACCTGC	2 cut(s)	724, 1006
PcsI	WCGNNNNNNNCGW	2 cut(s)	40, 1179
PflMI	CCANNNNNTGG	2 cut(s)	515, 533
PleI	GAGTC	2 cut(s)	1178, 1197
PluTI	GGCGCC	1 cut(s)	612
PpsI	GAGTC	2 cut(s)	1178, 1197
PpuMI	RGGWCCY	1 cut(s)	143
PshAI	GACNNNNGTC	1 cut(s)	1188
Psp124BI	GAGCTC	1 cut(s)	1015
Psp5II	RGGWCCY	1 cut(s)	143
Psp6I	CCWGG	6 cut(s)	292, 526, 628, 838, 856, 1165
PspFI	CCCAGC	7 cut(s)	51, 256, 522, 679, 751, 1058, 1080
PspGI	CCWGG	6 cut(s)	292, 526, 628, 838, 856, 1165
PspN4I	GGNNCC	8 cut(s)	30, 144, 387, 520, 588, 610, 956, 972
PspPI	GGNCC	8 cut(s)	29, 83, 143, 278, 296, 323, 536, 587
PspPPI	RGGWCCY	1 cut(s)	143
PspXI	VCTCGAGB	1 cut(s)	1186
PstI	CTGCAG	1 cut(s)	883
PstNI	CAGNNNCTG	3 cut(s)	758, 878, 1058
PsuI	RGATCY	1 cut(s)	970
PvuII	CAGCTG	3 cut(s)	755, 1019, 1058
RsaI	GTAC	4 cut(s)	100, 107, 767, 956
RsaNI	GTAC	4 cut(s)	99, 106, 766, 955
RseI	CAYNNNNRTG	4 cut(s)	216, 420, 432, 477
SacI	GAGCTC	1 cut(s)	1015
Sau3AI	GATC	2 cut(s)	970, 1114
Sau96I	GGNCC	8 cut(s)	29, 83, 143, 278, 296, 323, 536, 587
SbfI	CCTGCAGG	1 cut(s)	883
SchI	GAGTC	2 cut(s)	1178, 1198
ScrFI	CCNGG	6 cut(s)	294, 528, 630, 840, 858, 1167
SdaI	CCTGCAGG	1 cut(s)	883
SduI	GDGCHC	5 cut(s)	22, 43, 523, 1015, 1066
SfaNI	GCATC	3 cut(s)	192, 388, 713
SfcI	CTRYAG	2 cut(s)	373, 879
SfoI	GGCGCC	1 cut(s)	610
Sfr274I	CTCGAG	1 cut(s)	1186
SinI	GGWCC	6 cut(s)	29, 83, 143, 323, 536, 587
SlaI	CTCGAG	1 cut(s)	1186
SmiMI	CAYNNNNRTG	4 cut(s)	216, 420, 432, 477
SmlI	CTYRAG	1 cut(s)	1186
SmoI	CTYRAG	1 cut(s)	1186
Sse8387I	CCTGCAGG	1 cut(s)	883
Sse9I	AATT	2 cut(s)	124, 346
SsiI	CCGC	5 cut(s)	216, 638, 1050, 1127, 1137
SspDI	GGCGCC	1 cut(s)	608
SspMI	CTAG	2 cut(s)	147, 825
SstI	GAGCTC	1 cut(s)	1015
StyD4I	CCNGG	6 cut(s)	292, 526, 628, 838, 856, 1165
StyI	CCWWGG	1 cut(s)	615
TaaI	ACNGT	1 cut(s)	1153
TaiI	ACGT	1 cut(s)	905
TaqI	TCGA	2 cut(s)	788, 1187
TasI	AATT	2 cut(s)	124, 346
TscAI	CASTG	3 cut(s)	715, 946, 1158
TseFI	GTSAC	1 cut(s)	1155
Tsp45I	GTSAC	1 cut(s)	1155
TspDTI	ATGAA	3 cut(s)	51, 450, 924
TspGWI	ACGGA	1 cut(s)	726
TspRI	CASTG	3 cut(s)	715, 946, 1158
Van91I	CCANNNNNTGG	2 cut(s)	515, 533
VpaK11BI	GGWCC	6 cut(s)	29, 83, 143, 323, 536, 587
XceI	RCATGY	1 cut(s)	196
XcmI	CCANNNNNNNNNTGG	1 cut(s)	530
XhoI	CTCGAG	1 cut(s)	1186
XspI	CTAG	2 cut(s)	147, 825
Zsp2I	ATGCAT	1 cut(s)	706

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.