Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0008397

Physical Location & Seq

Genomic Coordinates Reverse (-)

64327317 .. 64332941

Gene Structure 5625 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G040590.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2451 bp

ATGGCGGAGGAGGAGGTTGTGGTGGTCTCCGCGAGCCCTAAGCCGTCGGATCACAAACGAAAGCTCGAGGAATTGGAGCCCGAACAAGAATCGGTCCTTGAACAGCCGCCTAAAAGCTCCGGCGAGCGTGCCGACTCGAATGCGGAGCCCGATGCAGCCAATGAACTTGATGCCTCGCCGCCGTCGGACGAATCTGACGCCAAACGGCCGCGTCTTGATGACAAGTCCGATGATATAGCCAGAGCAAATGGTCACCAGGAGAAGTTGGATGATACTCCGAAGGAGAATGATGAGGAAGAGGTAGTGCCCGGCCCTGCATTTGTTGACCTTACGGAAACAGAGATCGCTCCTTCTCAGCCTGGGTTTGTGGACCTTACGGAAACAGCAACGAACGAACAACTCTTTGGCACCAATGGTGAGACAAGTGACATCCAAAAGCCTTCTGTTGAGGATTCTGTGGTGGATGCTGCTCAGGAACCTCCTCAAGAGGAGCCTCAACCGCACTCCGCTGAGGAAGGGGACTCTGTCCCTGCACAACCAGAGCAAGGGGACGCTCTCTCTGGGCAACAAGAGAAAACAGATACTCTACCTGTGAACCAAGAGCAAGGTGATACTTTCTCTGGGCAACAGGAGCAAGGGGATGCCTTCTCTGCCCAACAGGATCAACTTCCTGAAAATGAGGCTATCACACACAGAATGGAGGTTCCTAATAATAAGGTTGGGGTTCTGATTGGTAAGGCTGGGGATACTATTCGATACTTGCAATACAATTCTAGAGCAAAAATCCAAATCACAAAGGATGCTGATGCGGATCCATATTCTGCAACAAGGCCTGTAGAGATAACAGGAAGTTTGGATAGTGTCACCAAAGCTGAAAAGCTTATAAATGCTGTTATTGCAGAGGCTGATGCTGGGGGTTCTCCTTCTCTGGTGGCTAGGGGCGTTGCTACCGCACAGGCTGTTGCAGCTGCAGAACAAATTCAGATACAAGTACCAAATGAAAAGGTTGGTTTGATAATAGGCAGAATGGGGGACACCATTAAAAGTCTGCAGGCCAAAACAGGGGCCCGTATCCAGTTGATACCCCAACATCTTCCAAAGGGAGATGAATCCAAAGAAAGGACAGTGCGAGTAAGTGGTGATAAGAAGCAAATTGACATGGCCAGGGAATTGATAAAGGAAGTTATGAATCAGACTGTGAGGCCATCCAGTGGTTTTAACCATCAGGCTTATCGGTCCCATGGACCAGGTGGTCCTCAATGGGGTCCACGGGGTGCTCGTCCATCCCAGCACTCTTCATATGATTATCAGCAACGAGGACCATATCAGTCCCGTACTCAGTACCCTCCTGCATATGGGAATTATCCTCAACATATGGGTCCTAGAAGTGGCTTTGGTTCTGGTTGGGAGCAAAGGCCACCTCCAAACATGCAGGGGATGCCTCCACATGGCGGTGGTTATGATTACTATGGCGGACAAGGAGCTGAAGCCCCAGCCCAACATTCTACTCATGGCCCTTCTCATGCTCCTGGCCCTTCTCCTAACCCTATAATGGGTGGACCTCCATCCCAAGCAAACTATAATTATGGACAGCCACATGGTTCAGACTATAGGCATCCGGCTCCTTTTTCTCATGCTGCACCCCCTCAGCATGGCTATGGGCATGGGTATGAAGAACCAAAATATGATAATCATGCTCCACCACAGCACTCATATGGAGGACATGGAGCTTCTCAGCCGTATCCACAAACTGGAGCTCAGCCAGGTTATGGTCCACAGCAGCATTATGGTAAGCCACCAGCATATACCATGCCATCTCAGGGACCACCTCCCCAGTCTTATGGCGCTCCCATGGGTAGCCAACCAGGAGAAGCGGTTTATCAGACACCAGCTCAGTCATATGGTCAGAATGTCCCAGCTCAACAGCCATATCCATATGCATCCACTGCACCTGCACAGCAGACTTATGCTCCGTATGGTTCTACCCCAGCTGCTGATGGGTACACTCAGCCACCAACTGCTTCGAGCGCAGGTTATCCACAGCAAGGAGGGCAGCCTGCTAGTTATGGTCAGCCTGGCTCGCAGCCTGCACCTGGCTATGCACAAGCAGCTCCTGCAGGGTATGCACAATACCCAACGTCTCAACAAGGTTACTCCGAGCAGTCTGCTTCATACACTGGTGGTTATGGGTACCAAGCGTCTCAGGATCCTGCCTATGGAGGTGTCTCTGCACCTGCCTATGGTGGAGCTCCAGCTGCGCAACAGGGCTACGCACAACCAGCACCCGCAGCAGCTGGGGCACAGCAGGGCTATGCCCAGCCAGCACCAGCAACAACTCAACAAAGCTATGATCAATCGATCCCGCAGTCAGGCGGTTATGGGAAAACAGTGTCACCTCAGCCTGGCTACGCTCAATACGACTCGAGTCAAATGTATGCTGCACCTCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1

Protein Analysis

816

Amino Acids

86.47

Weight (kDa)

4.84

Isoelectric Point (pI)

60.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KH_1	PF00013	230 - 296	3.6e-15	KH domain
KH_1	PF00013	327 - 394	3.1e-18	KH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008397)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33680 AT4G10070
fragaria_vesca	FvH4_2g35080 FvH4_2g35080 FvH4_2g35080
malus_domestica	MD08G1108100.v1.1 MD15G1088600.v1.1 MD15G1088800.v1.1
prunus_persica	Prupe.1G442700_v2.0.a1
pyrus_communis	pycom08g09010 pycom15g08310
rosa_chinensis	RchiOBHm_Chr6g0306891
rosa_laevigata	RLG00000010757
rosa_multiflora	Rmu_co8448611.1_g000001 Rmu_co8474845.1_g000001 Rmu_sc0018770.1_g000003
rosa_roxburghii	Rroxscaffold_7G00161380
rosa_rugosa	Rorug06G0354100
rosa_samantha	Rh6AG465700 Rh6BG435100 Rh6CG480200 Rh6DG467100
rosa_wichuraiana	Rw6G040590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	884
AarI	CACCTGC	2 cut(s)	1960, 2242
AasI	GACNNNNNNGTC	1 cut(s)	1251
Acc16I	TGCGCA	1 cut(s)	2259
Acc36I	ACCTGC	3 cut(s)	1960, 2021, 2242
Acc65I	GGTACC	1 cut(s)	2190
AccB1I	GGYRCC	2 cut(s)	407, 2190
AccB7I	CCANNNNNTGG	3 cut(s)	1211, 1751, 1769
AccII	CGCG	2 cut(s)	32, 211
AclWI	GGATC	7 cut(s)	57, 669, 806, 819, 2201, 2214, 2353
AcoI	YGGCCR	2 cut(s)	206, 1161
AcsI	RAATTY	1 cut(s)	978
AcuI	CTGAAG	1 cut(s)	1506
AcyI	GRCGYC	1 cut(s)	198
AdeI	CACNNNGTG	1 cut(s)	1274
AfaI	GTAC	5 cut(s)	993, 1336, 1343, 2003, 2192
AgsI	TTSAA	1 cut(s)	101
AjuI	GAANNNNNNNTTGG	2 cut(s)	387, 419
Alw21I	GWGCWC	3 cut(s)	1279, 1759, 2251
Alw26I	GTCTC	5 cut(s)	31, 413, 2145, 2205, 2230
AlwI	GGATC	7 cut(s)	57, 669, 806, 819, 2201, 2214, 2353
AlwNI	CAGNNNCTG	4 cut(s)	905, 1994, 2114, 2294
Ama87I	CYCGRG	2 cut(s)	65, 2422
ApaI	GGGCCC	1 cut(s)	1069
ApoI	RAATTY	1 cut(s)	978
Asp700I	GAANNNNTTC	1 cut(s)	978
Asp718I	GGTACC	1 cut(s)	2190
AspLEI	GCGC	3 cut(s)	1847, 2030, 2260
AsuC2I	CCSGG	1 cut(s)	309
AsuHPI	GGTGA	6 cut(s)	245, 428, 620, 856, 1151, 2385
AvaI	CYCGRG	2 cut(s)	65, 2422
BaeGI	GKGCMC	3 cut(s)	309, 1069, 2302
BalI	TGGCCA	1 cut(s)	1163
BamHI	GGATCC	2 cut(s)	811, 2206
BanI	GGYRCC	2 cut(s)	407, 2190
BanII	GRGCYC	6 cut(s)	38, 81, 150, 1069, 1759, 2251
Bbv12I	GWGCWC	3 cut(s)	1279, 1759, 2251
BbvCI	CCTCAGC	3 cut(s)	510, 1647, 2397
BccI	CCATC	6 cut(s)	1213, 1230, 1291, 1573, 1822, 1991
BceAI	ACGGC	4 cut(s)	28, 166, 221, 1723
BcgI	CGANNNNNNTGC	4 cut(s)	122, 156, 2147, 2181
BciVI	GTATCC	3 cut(s)	739, 1082, 1752
BclI	TGATCA	1 cut(s)	2350
BcnI	CCSGG	1 cut(s)	309
BcoDI	GTCTC	5 cut(s)	31, 413, 2145, 2205, 2230
BfaI	CTAG	4 cut(s)	774, 936, 1383, 2061
BfmI	CTRYAG	5 cut(s)	834, 969, 1049, 1609, 2115
BfoI	RGCGCY	1 cut(s)	1848
BfuAI	ACCTGC	3 cut(s)	1960, 2021, 2242
BfuI	GTATCC	3 cut(s)	739, 1082, 1752
BlpI	GCTNAGC	1 cut(s)	1758
BmeT110I	CYCGRG	2 cut(s)	65, 2422
BmrI	ACTGGG	1 cut(s)	1828
BmuI	ACTGGG	1 cut(s)	1828
BoxI	GACNNNNGTC	1 cut(s)	2424
BpmI	CTGGAG	2 cut(s)	1773, 2235
Bpu10I	CCTNAGC	5 cut(s)	39, 471, 510, 1647, 2397
Bpu1102I	GCTNAGC	1 cut(s)	1758
BpuEI	CTTGAG	1 cut(s)	468
BpuMI	CCSGG	1 cut(s)	309
Bsa29I	ATCGAT	1 cut(s)	2357
BsaBI	GATNNNNATC	1 cut(s)	810
BsaHI	GRCGYC	1 cut(s)	198
BsaI	GGTCTC	1 cut(s)	31
BsaJI	CCNNGG	5 cut(s)	359, 1164, 1240, 1268, 1851
BsaXI	ACNNNNNCTCC	4 cut(s)	1860, 1890, 1954, 1984
Bse1I	ACTGG	5 cut(s)	1075, 1209, 1756, 1834, 2182
Bse8I	GATNNNNATC	1 cut(s)	810
BseCI	ATCGAT	1 cut(s)	2357
BseDI	CCNNGG	5 cut(s)	359, 1164, 1240, 1268, 1851
BseJI	GATNNNNATC	1 cut(s)	810
BseNI	ACTGG	5 cut(s)	1075, 1209, 1756, 1834, 2182
BseRI	GAGGAG	4 cut(s)	23, 26, 471, 503
BseSI	GKGCMC	3 cut(s)	309, 1069, 2302
BseX3I	CGGCCG	1 cut(s)	206
BseYI	CCCAGC	8 cut(s)	740, 911, 1287, 1492, 1915, 1987, 2294, 2316
BsgI	GTGCAG	7 cut(s)	516, 1623, 1932, 1938, 2073, 2214, 2424
Bsh1236I	CGCG	2 cut(s)	32, 211
Bsh1285I	CGRYCG	1 cut(s)	209
BshNI	GGYRCC	2 cut(s)	407, 2190
BshVI	ATCGAT	1 cut(s)	2357
BsiEI	CGRYCG	1 cut(s)	209
BsiHKAI	GWGCWC	3 cut(s)	1279, 1759, 2251
BsiHKCI	CYCGRG	2 cut(s)	65, 2422
BsiSI	CCGG	3 cut(s)	120, 309, 1619
BslFI	GGGAC	8 cut(s)	512, 533, 563, 1046, 1222, 1315, 1836, 1898
BsmAI	GTCTC	5 cut(s)	31, 413, 2145, 2205, 2230
BsmBI	CGTCTC	2 cut(s)	2145, 2205
BsmFI	GGGAC	8 cut(s)	512, 533, 563, 1046, 1222, 1315, 1836, 1898
BsmI	GAATGC	1 cut(s)	145
Bso31I	GGTCTC	1 cut(s)	31
BsoBI	CYCGRG	2 cut(s)	65, 2422
Bsp120I	GGGCCC	1 cut(s)	1065
Bsp1286I	GDGCHC	9 cut(s)	38, 81, 150, 309, 1069, 1279, 1759, 2251, 2302
Bsp143I	GATC	7 cut(s)	49, 342, 661, 811, 2206, 2350, 2358
Bsp1720I	GCTNAGC	1 cut(s)	1758
Bsp19I	CCATGG	2 cut(s)	1240, 1851
BspDI	ATCGAT	1 cut(s)	2357
BspFNI	CGCG	2 cut(s)	32, 211
BspMAI	CTGCAG	3 cut(s)	973, 1053, 2119
BspMI	ACCTGC	3 cut(s)	1960, 2021, 2242
BspPI	GGATC	7 cut(s)	57, 669, 806, 819, 2201, 2214, 2353
BspT107I	GGYRCC	2 cut(s)	407, 2190
BspTNI	GGTCTC	1 cut(s)	31
BsrI	ACTGG	5 cut(s)	1075, 1209, 1756, 1834, 2182
BssECI	CCNNGG	5 cut(s)	359, 1164, 1240, 1268, 1851
BssMI	GATC	7 cut(s)	49, 342, 661, 811, 2206, 2350, 2358
BssNI	GRCGYC	1 cut(s)	198
BssT1I	CCWWGG	2 cut(s)	1240, 1851
Bst4CI	ACNGT	3 cut(s)	1126, 1198, 2389
Bst6I	CTCTTC	2 cut(s)	291, 1300
BstACI	GRCGYC	1 cut(s)	198
BstAPI	GCANNNNNTGC	4 cut(s)	1438, 1946, 2114, 2123
BstC8I	GCNNGC	8 cut(s)	34, 125, 129, 1053, 2058, 2081, 2088, 2322
BstDSI	CCRYGG	3 cut(s)	1240, 1268, 1851
BstEII	GGTNACC	1 cut(s)	251
BstFNI	CGCG	2 cut(s)	32, 211
BstH2I	RGCGCY	1 cut(s)	1848
BstHHI	GCGC	3 cut(s)	1847, 2030, 2260
BstKTI	GATC	7 cut(s)	52, 345, 664, 814, 2209, 2353, 2361
BstMAI	GTCTC	5 cut(s)	31, 413, 2145, 2205, 2230
BstMBI	GATC	7 cut(s)	49, 342, 661, 811, 2206, 2350, 2358
BstMCI	CGRYCG	1 cut(s)	209
BstNSI	RCATGY	1 cut(s)	1432
BstPAI	GACNNNNGTC	1 cut(s)	2424
BstPI	GGTNACC	1 cut(s)	251
BstSFI	CTRYAG	5 cut(s)	834, 969, 1049, 1609, 2115
BstSLI	GKGCMC	3 cut(s)	309, 1069, 2302
BstUI	CGCG	2 cut(s)	32, 211
BstX2I	RGATCY	2 cut(s)	811, 2206
BstXI	CCANNNNNNTGG	1 cut(s)	1841
BstYI	RGATCY	2 cut(s)	811, 2206
BstZI	CGGCCG	1 cut(s)	206
Bsu15I	ATCGAT	1 cut(s)	2357
BsuI	GTATCC	3 cut(s)	739, 1082, 1752
BsuTUI	ATCGAT	1 cut(s)	2357
BtgI	CCRYGG	3 cut(s)	1240, 1268, 1851
BtsI	GCAGTG	1 cut(s)	1944
BtsIMutI	CAGTG	5 cut(s)	1131, 1216, 1944, 2175, 2394
BveI	ACCTGC	3 cut(s)	1960, 2021, 2242
Cac8I	GCNNGC	8 cut(s)	34, 125, 129, 1053, 2058, 2081, 2088, 2322
CaiI	CAGNNNCTG	4 cut(s)	905, 1994, 2114, 2294
CfoI	GCGC	3 cut(s)	1847, 2030, 2260
ClaI	ATCGAT	1 cut(s)	2357
CseI	GACGC	4 cut(s)	200, 206, 560, 2187
CsiI	ACCWGGT	1 cut(s)	1246
Csp6I	GTAC	5 cut(s)	992, 1335, 1342, 2002, 2191
CviQI	GTAC	5 cut(s)	992, 1335, 1342, 2002, 2191
DpnI	GATC	7 cut(s)	51, 344, 663, 813, 2208, 2352, 2360
DpnII	GATC	7 cut(s)	49, 342, 661, 811, 2206, 2350, 2358
DraIII	CACNNNGTG	1 cut(s)	1274
DrdI	GACNNNNNNGTC	1 cut(s)	1251
DseDI	GACNNNNNNGTC	1 cut(s)	1251
EaeI	YGGCCR	2 cut(s)	206, 1161
EagI	CGGCCG	1 cut(s)	206
Eam1104I	CTCTTC	2 cut(s)	291, 1300
EarI	CTCTTC	2 cut(s)	291, 1300
EciI	GGCGGA	2 cut(s)	20, 1488
Ecl136II	GAGCTC	2 cut(s)	1757, 2249
EclXI	CGGCCG	1 cut(s)	206
Eco130I	CCWWGG	2 cut(s)	1240, 1851
Eco147I	AGGCCT	1 cut(s)	832
Eco24I	GRGCYC	6 cut(s)	38, 81, 150, 1069, 1759, 2251
Eco31I	GGTCTC	1 cut(s)	31
Eco52I	CGGCCG	1 cut(s)	206
Eco53kI	GAGCTC	2 cut(s)	1757, 2249
Eco57I	CTGAAG	1 cut(s)	1506
Eco88I	CYCGRG	2 cut(s)	65, 2422
Eco91I	GGTNACC	1 cut(s)	251
EcoICRI	GAGCTC	2 cut(s)	1757, 2249
EcoO109I	RGGNCCY	2 cut(s)	1065, 1379
EcoO65I	GGTNACC	1 cut(s)	251
EcoT14I	CCWWGG	2 cut(s)	1240, 1851
EcoT22I	ATGCAT	1 cut(s)	1942
EcoT38I	GRGCYC	6 cut(s)	38, 81, 150, 1069, 1759, 2251
ErhI	CCWWGG	2 cut(s)	1240, 1851
Esp3I	CGTCTC	2 cut(s)	2145, 2205
FaqI	GGGAC	8 cut(s)	512, 533, 563, 1046, 1222, 1315, 1836, 1898
FauI	CCCGC	2 cut(s)	2293, 2370
FauNDI	CATATG	6 cut(s)	1300, 1354, 1374, 1714, 1900, 1936
FbaI	TGATCA	1 cut(s)	2350
FriOI	GRGCYC	6 cut(s)	38, 81, 150, 1069, 1759, 2251
FspBI	CTAG	4 cut(s)	774, 936, 1383, 2061
FspI	TGCGCA	1 cut(s)	2259
GlaI	GCGC	3 cut(s)	1846, 2029, 2259
GsaI	CCCAGC	8 cut(s)	744, 915, 1291, 1496, 1919, 1991, 2298, 2320
GsuI	CTGGAG	2 cut(s)	1773, 2235
HaeII	RGCGCY	1 cut(s)	1848
HapII	CCGG	3 cut(s)	120, 309, 1619
HgaI	GACGC	4 cut(s)	200, 206, 560, 2187
HhaI	GCGC	3 cut(s)	1847, 2030, 2260
Hin1I	GRCGYC	1 cut(s)	198
Hin6I	GCGC	3 cut(s)	1845, 2028, 2258
HinP1I	GCGC	3 cut(s)	1845, 2028, 2258
HincII	GTYRAC	1 cut(s)	325
HindII	GTYRAC	1 cut(s)	325
HindIII	AAGCTT	1 cut(s)	878
HinfI	GANTC	9 cut(s)	89, 134, 191, 452, 521, 1109, 1189, 2420, 2425
HpaII	CCGG	3 cut(s)	120, 309, 1619
HphI	GGTGA	6 cut(s)	245, 428, 620, 856, 1151, 2385
Hpy166II	GTNNAC	7 cut(s)	325, 370, 595, 1268, 1559, 1775, 2004
Hpy188III	TCNNGA	6 cut(s)	215, 473, 485, 671, 774, 2204
Hpy8I	GTNNAC	7 cut(s)	325, 370, 595, 1268, 1559, 1775, 2004
Hpy99I	CGWCG	2 cut(s)	49, 187
HpyAV	CCTTC	8 cut(s)	274, 360, 450, 509, 655, 933, 1527, 1545
HpyCH4III	ACNGT	3 cut(s)	1126, 1198, 2389
HpyCH4IV	ACGT	1 cut(s)	2138
HpySE526I	ACGT	1 cut(s)	2138
Hsp92I	GRCGYC	1 cut(s)	198
HspAI	GCGC	3 cut(s)	1845, 2028, 2258
KpnI	GGTACC	1 cut(s)	2194
Ksp22I	TGATCA	1 cut(s)	2350
Kzo9I	GATC	7 cut(s)	49, 342, 661, 811, 2206, 2350, 2358
MabI	ACCWGGT	1 cut(s)	1246
MaeI	CTAG	4 cut(s)	774, 936, 1383, 2061
MaeII	ACGT	1 cut(s)	2138
MaeIII	GTNAC	5 cut(s)	251, 425, 862, 2150, 2391
MalI	GATC	7 cut(s)	51, 344, 663, 813, 2208, 2352, 2360
MboI	GATC	7 cut(s)	49, 342, 661, 811, 2206, 2350, 2358
MboII	GAAGA	4 cut(s)	308, 1085, 1287, 1685
MflI	RGATCY	2 cut(s)	811, 2206
MhlI	GDGCHC	9 cut(s)	38, 81, 150, 309, 1069, 1279, 1759, 2251, 2302
MlsI	TGGCCA	1 cut(s)	1163
MluCI	AATT	7 cut(s)	71, 769, 978, 1152, 1169, 1360, 1582
MluNI	TGGCCA	1 cut(s)	1163
MlyI	GAGTC	4 cut(s)	128, 515, 2414, 2434
MmeI	TCCRAC	3 cut(s)	27, 165, 246
Mox20I	TGGCCA	1 cut(s)	1163
Mph1103I	ATGCAT	1 cut(s)	1942
MroXI	GAANNNNTTC	1 cut(s)	978
MscI	TGGCCA	1 cut(s)	1163
MseI	TTAA	2 cut(s)	1041, 1218
MslI	CAYNNNNRTG	4 cut(s)	1452, 1656, 1668, 1713
Msp20I	TGGCCA	1 cut(s)	1163
MspA1I	CMGCKG	5 cut(s)	509, 968, 1991, 2255, 2294
MspI	CCGG	3 cut(s)	120, 309, 1619
Mva1269I	GAATGC	1 cut(s)	145
MvnI	CGCG	2 cut(s)	32, 211
NciI	CCSGG	1 cut(s)	309
NcoI	CCATGG	2 cut(s)	1240, 1851
NdeI	CATATG	6 cut(s)	1300, 1354, 1374, 1714, 1900, 1936
NdeII	GATC	7 cut(s)	49, 342, 661, 811, 2206, 2350, 2358
NmuCI	GTSAC	4 cut(s)	251, 425, 862, 2391
NsbI	TGCGCA	1 cut(s)	2259
NsiI	ATGCAT	1 cut(s)	1942
NspI	RCATGY	1 cut(s)	1432
PaeR7I	CTCGAG	2 cut(s)	65, 2422
PaqCI	CACCTGC	2 cut(s)	1960, 2242
PceI	AGGCCT	1 cut(s)	832
PcsI	WCGNNNNNNNCGW	2 cut(s)	1276, 2415
PctI	GAATGC	1 cut(s)	145
PdmI	GAANNNNTTC	1 cut(s)	978
PfeI	GAWTC	5 cut(s)	89, 191, 452, 1109, 1189
PflFI	GACNNNGTC	1 cut(s)	524
PflMI	CCANNNNNTGG	3 cut(s)	1211, 1751, 1769
PleI	GAGTC	4 cut(s)	128, 515, 2414, 2433
PpsI	GAGTC	4 cut(s)	128, 515, 2414, 2433
PpuMI	RGGWCCY	1 cut(s)	1379
PshAI	GACNNNNGTC	1 cut(s)	2424
PsiI	TTATAA	1 cut(s)	884
Psp124BI	GAGCTC	2 cut(s)	1759, 2251
Psp5II	RGGWCCY	1 cut(s)	1379
PspEI	GGTNACC	1 cut(s)	251
PspFI	CCCAGC	8 cut(s)	740, 911, 1287, 1492, 1915, 1987, 2294, 2316
PspOMI	GGGCCC	1 cut(s)	1065
PspPPI	RGGWCCY	1 cut(s)	1379
PspXI	VCTCGAGB	2 cut(s)	65, 2422
PstI	CTGCAG	3 cut(s)	973, 1053, 2119
PstNI	CAGNNNCTG	4 cut(s)	905, 1994, 2114, 2294
PsuI	RGATCY	2 cut(s)	811, 2206
PsyI	GACNNNGTC	1 cut(s)	524
PvuII	CAGCTG	4 cut(s)	968, 1991, 2255, 2294
RsaI	GTAC	5 cut(s)	993, 1336, 1343, 2003, 2192
RsaNI	GTAC	5 cut(s)	992, 1335, 1342, 2002, 2191
RseI	CAYNNNNRTG	4 cut(s)	1452, 1656, 1668, 1713
SacI	GAGCTC	2 cut(s)	1759, 2251
SaqAI	TTAA	2 cut(s)	1041, 1218
Sau3AI	GATC	7 cut(s)	49, 342, 661, 811, 2206, 2350, 2358
SbfI	CCTGCAGG	1 cut(s)	2119
SchI	GAGTC	4 cut(s)	128, 515, 2414, 2434
SdaI	CCTGCAGG	1 cut(s)	2119
SduI	GDGCHC	9 cut(s)	38, 81, 150, 309, 1069, 1279, 1759, 2251, 2302
SexAI	ACCWGGT	1 cut(s)	1246
SfcI	CTRYAG	5 cut(s)	834, 969, 1049, 1609, 2115
Sfr274I	CTCGAG	2 cut(s)	65, 2422
SlaI	CTCGAG	2 cut(s)	65, 2422
SmiMI	CAYNNNNRTG	4 cut(s)	1452, 1656, 1668, 1713
SmlI	CTYRAG	3 cut(s)	65, 483, 2422
SmoI	CTYRAG	3 cut(s)	65, 483, 2422
Sse8387I	CCTGCAGG	1 cut(s)	2119
Sse9I	AATT	7 cut(s)	71, 769, 978, 1152, 1169, 1360, 1582
SseBI	AGGCCT	1 cut(s)	832
SspMI	CTAG	4 cut(s)	774, 936, 1383, 2061
SstI	GAGCTC	2 cut(s)	1759, 2251
StuI	AGGCCT	1 cut(s)	832
StyI	CCWWGG	2 cut(s)	1240, 1851
TaaI	ACNGT	3 cut(s)	1126, 1198, 2389
TaiI	ACGT	1 cut(s)	2141
TaqI	TCGA	6 cut(s)	66, 137, 754, 2024, 2357, 2423
TaqII	GACCGA	2 cut(s)	82, 1224
TasI	AATT	7 cut(s)	71, 769, 978, 1152, 1169, 1360, 1582
TauI	GCSGC	3 cut(s)	109, 181, 211
TfiI	GAWTC	5 cut(s)	89, 191, 452, 1109, 1189
Tru1I	TTAA	2 cut(s)	1041, 1218
Tru9I	TTAA	2 cut(s)	1041, 1218
TscAI	CASTG	5 cut(s)	1131, 1216, 1951, 2182, 2394
TseFI	GTSAC	4 cut(s)	251, 425, 862, 2391
Tsp45I	GTSAC	4 cut(s)	251, 425, 862, 2391
TspDTI	ATGAA	7 cut(s)	177, 1014, 1122, 1202, 1287, 1686, 2160
TspGWI	ACGGA	3 cut(s)	347, 392, 1962
TspRI	CASTG	5 cut(s)	1131, 1216, 1951, 2182, 2394
Tth111I	GACNNNGTC	1 cut(s)	524
Van91I	CCANNNNNTGG	3 cut(s)	1211, 1751, 1769
XapI	RAATTY	1 cut(s)	978
XbaI	TCTAGA	1 cut(s)	773
XceI	RCATGY	1 cut(s)	1432
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1247
XhoI	CTCGAG	2 cut(s)	65, 2422
XmnI	GAANNNNTTC	1 cut(s)	978
XspI	CTAG	4 cut(s)	774, 936, 1383, 2061
Zsp2I	ATGCAT	1 cut(s)	1942

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.