Rmu_sc0000082.1_g000088

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000082.1
OG0018160
Physical Location & Seq
Forward (+)
303066 .. 305190
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 999 bp
atggattttccattggagttattaattaagggccatgtattttttaatggcattgtgactagttataagaattggaaatggcatggagaaccttctgtcaagaatactgatagagtaggtgaatctgaaatagtagatatgaattgtaaccaagatatgggggaaagtgaggatgaggagatatctagtaactcgaatgagtttctaaggtttgtggaagatggagataaacctttgtacccctgttgtacaaagacaacaaagctgaactgtcttattcaaacgttcaacttgaaagcaaaacacggaatgactgatgcttgttattccgacatgctagtcatgattggcaggttgcttcctgaagggaatgaggcggtttcgccaccatccggccaccccaggacgggccgttcggcttggtgtgatcctctcctccttctctacttagtcatattctttgggggttttggttgtcggggaagtgctgaaagtgttcctaaccttgttgcagctcgttttgatcggcagtccctcccagactacatcccgatcggcagtcccgtccgatgcgtcaccgggttggcagtctgtgatgccccggaaattcgggggtggcaatggttgaatttttatacgttgggattctccgaaatcttccttcatgaaagtcatagagcgcgtcgatacgagttcgtggacatgtggaaatcggcaatcggaattctctccattttctttcctcccctcgcggccgaactgctcgacccgacccggaaatatctccagcctccggcgtcctccggtcccggacccggccatctccggactcgccatgagccgcccagtcgccccgtggcgtcagcttgccccgccgctgcccccagagctggatcgaagacaccacagccgtgcgaaacccaacccggccggagatccccgattccggcgttgcacgggccgatccttctcagttttggaatctcctcttccccctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21

Protein Analysis

332

Amino Acids

36.66

Weight (kDa)

6.31

Isoelectric Point (pI)

52.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 66
AasI GACNNNNNNGTC 1 cut(s) 338
Acc36I ACCTGC 1 cut(s) 342
AccB7I CCANNNNNTGG 1 cut(s) 157
AccII CGCG 2 cut(s) 682, 752
AccIII TCCGGA 1 cut(s) 825
AciI CCGC 5 cut(s) 377, 752, 842, 873, 876
AclI AACGTT 1 cut(s) 284
AclWI GGATC 4 cut(s) 422, 901, 930, 958
AcoI YGGCCR 4 cut(s) 394, 753, 817, 928
AcsI RAATTY 3 cut(s) 606, 628, 723
AcuI CTGAAG 1 cut(s) 384
AcyI GRCGYC 2 cut(s) 797, 860
AfaI GTAC 2 cut(s) 239, 250
AfiI CCNNNNNNNGG 8 cut(s) 157, 392, 406, 407, 793, 815, 890, 946
AflIII ACRYGT 1 cut(s) 702
AgsI TTSAA 4 cut(s) 281, 289, 295, 628
AhlI ACTAGT 1 cut(s) 59
AjnI CCWGG 1 cut(s) 401
AleI CACNNNNGTG 1 cut(s) 910
AloI GAACNNNNNNTCC 2 cut(s) 397, 429
AluBI AGCT 4 cut(s) 265, 515, 866, 890
AluI AGCT 4 cut(s) 265, 515, 866, 890
AlwI GGATC 4 cut(s) 422, 901, 930, 958
Aor13HI TCCGGA 1 cut(s) 825
AoxI GGCC 7 cut(s) 31, 394, 409, 753, 817, 928, 959
ApeKI GCWGC 2 cut(s) 512, 878
ApoI RAATTY 3 cut(s) 606, 628, 723
ArsI GACNNNNNNTTYG 2 cut(s) 397, 429
AseI ATTAAT 1 cut(s) 23
Asp700I GAANNNNTTC 1 cut(s) 495
AspLEI GCGC 1 cut(s) 682
AspS9I GGNCC 5 cut(s) 31, 409, 806, 812, 959
AsuC2I CCSGG 6 cut(s) 580, 602, 775, 810, 816, 927
AsuHPI GGTGA 2 cut(s) 131, 568
AvaII GGWCC 2 cut(s) 806, 812
BaeI ACNNNNGTAYC 2 cut(s) 221, 254
BbsI GAAGAC 1 cut(s) 905
BbvI GCAGC 2 cut(s) 524, 865
BccI CCATC 3 cut(s) 215, 397, 828
BceAI ACGGC 2 cut(s) 396, 895
BciT130I CCWGG 1 cut(s) 403
BcnI CCSGG 6 cut(s) 580, 602, 775, 810, 816, 927
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 3 cut(s) 60, 186, 338
BfuAI ACCTGC 1 cut(s) 342
BisI GCNGC 5 cut(s) 513, 753, 842, 876, 879
BlsI GCNGC 5 cut(s) 514, 754, 843, 877, 880
Bme1390I CCNGG 7 cut(s) 403, 580, 602, 775, 810, 816, 927
Bme18I GGWCC 2 cut(s) 806, 812
BmgT120I GGNCC 5 cut(s) 31, 409, 806, 812, 959
BmiI GGNNCC 2 cut(s) 808, 814
BmrFI CCNGG 7 cut(s) 403, 580, 602, 775, 810, 816, 927
BmrI ACTGGG 1 cut(s) 840
BmsI GCATC 3 cut(s) 307, 560, 586
BmuI ACTGGG 1 cut(s) 840
BpiI GAAGAC 1 cut(s) 905
BpmI CTGGAG 1 cut(s) 770
BpuMI CCSGG 6 cut(s) 580, 602, 775, 810, 816, 927
BsaHI GRCGYC 2 cut(s) 797, 860
BsaJI CCNNGG 3 cut(s) 401, 600, 855
BsaWI WCCGGW 2 cut(s) 803, 825
Bsc4I CCNNNNNNNGG 8 cut(s) 157, 392, 406, 407, 793, 815, 890, 946
Bse1I ACTGG 1 cut(s) 846
Bse3DI GCAATG 1 cut(s) 626
BseAI TCCGGA 1 cut(s) 825
BseBI CCWGG 1 cut(s) 403
BseDI CCNNGG 3 cut(s) 401, 600, 855
BseGI GGATG 3 cut(s) 178, 389, 546
BseLI CCNNNNNNNGG 8 cut(s) 157, 392, 406, 407, 793, 815, 890, 946
BseMI GCAATG 1 cut(s) 626
BseMII CTCAG 1 cut(s) 985
BseNI ACTGG 1 cut(s) 846
BseRI GAGGAG 3 cut(s) 191, 425, 976
BseX3I CGGCCG 2 cut(s) 753, 928
BseXI GCAGC 2 cut(s) 524, 865
Bsh1236I CGCG 2 cut(s) 682, 752
Bsh1285I CGRYCG 3 cut(s) 555, 756, 931
BshFI GGCC 7 cut(s) 33, 396, 411, 755, 819, 930, 961
BsiEI CGRYCG 3 cut(s) 555, 756, 931
BslFI GGGAC 3 cut(s) 517, 546, 792
BslI CCNNNNNNNGG 8 cut(s) 157, 392, 406, 407, 793, 815, 890, 946
BsmFI GGGAC 3 cut(s) 517, 546, 792
BsnI GGCC 7 cut(s) 33, 396, 411, 755, 819, 930, 961
Bsp13I TCCGGA 1 cut(s) 825
Bsp1407I TGTACA 1 cut(s) 248
Bsp143I GATC 6 cut(s) 427, 523, 552, 893, 935, 963
BspACI CCGC 5 cut(s) 377, 752, 842, 873, 876
BspANI GGCC 7 cut(s) 33, 396, 411, 755, 819, 930, 961
BspCNI CTCAG 1 cut(s) 984
BspEI TCCGGA 1 cut(s) 825
BspFNI CGCG 2 cut(s) 682, 752
BspHI TCATGA 2 cut(s) 342, 664
BspLI GGNNCC 2 cut(s) 808, 814
BspMI ACCTGC 1 cut(s) 342
BspPI GGATC 4 cut(s) 422, 901, 930, 958
BsrDI GCAATG 1 cut(s) 626
BsrGI TGTACA 1 cut(s) 248
BsrI ACTGG 1 cut(s) 846
BssECI CCNNGG 3 cut(s) 401, 600, 855
BssMI GATC 6 cut(s) 427, 523, 552, 893, 935, 963
BssNI GRCGYC 2 cut(s) 797, 860
Bst2UI CCWGG 1 cut(s) 403
Bst4CI ACNGT 1 cut(s) 272
Bst6I CTCTTC 1 cut(s) 994
BstACI GRCGYC 2 cut(s) 797, 860
BstAUI TGTACA 1 cut(s) 248
BstC8I GCNNGC 1 cut(s) 868
BstDEI CTNAG 3 cut(s) 206, 448, 971
BstDSI CCRYGG 1 cut(s) 855
BstF5I GGATG 3 cut(s) 178, 389, 546
BstFNI CGCG 2 cut(s) 682, 752
BstHHI GCGC 1 cut(s) 682
BstKTI GATC 6 cut(s) 430, 526, 555, 896, 938, 966
BstMBI GATC 6 cut(s) 427, 523, 552, 893, 935, 963
BstMCI CGRYCG 3 cut(s) 555, 756, 931
BstMWI GCNNNNNNNGC 2 cut(s) 872, 887
BstNI CCWGG 1 cut(s) 403
BstNSI RCATGY 2 cut(s) 337, 706
BstSCI CCNGG 7 cut(s) 401, 578, 600, 773, 808, 814, 925
BstUI CGCG 2 cut(s) 682, 752
BstV1I GCAGC 2 cut(s) 524, 865
BstV2I GAAGAC 1 cut(s) 905
BstX2I RGATCY 1 cut(s) 935
BstYI RGATCY 1 cut(s) 935
BstZI CGGCCG 2 cut(s) 753, 928
BsuRI GGCC 7 cut(s) 33, 396, 411, 755, 819, 930, 961
BtgI CCRYGG 1 cut(s) 855
BtsCI GGATG 3 cut(s) 178, 389, 546
BveI ACCTGC 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 868
CciI TCATGA 2 cut(s) 342, 664
CfoI GCGC 1 cut(s) 682
Cfr13I GGNCC 5 cut(s) 31, 409, 806, 812, 959
CseI GACGC 4 cut(s) 562, 671, 786, 849
Csp6I GTAC 2 cut(s) 238, 249
CviAII CATG 7 cut(s) 35, 83, 334, 343, 665, 703, 836
CviQI GTAC 2 cut(s) 238, 249
DdeI CTNAG 3 cut(s) 206, 448, 971
DpnI GATC 6 cut(s) 429, 525, 554, 895, 937, 965
DpnII GATC 6 cut(s) 427, 523, 552, 893, 935, 963
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
EaeI YGGCCR 4 cut(s) 394, 753, 817, 928
EagI CGGCCG 2 cut(s) 753, 928
Eam1104I CTCTTC 1 cut(s) 994
EarI CTCTTC 1 cut(s) 994
EclXI CGGCCG 2 cut(s) 753, 928
Eco32I GATATC 1 cut(s) 183
Eco47I GGWCC 2 cut(s) 806, 812
Eco52I CGGCCG 2 cut(s) 753, 928
Eco57I CTGAAG 1 cut(s) 384
EcoRI GAATTC 1 cut(s) 723
EcoRII CCWGG 1 cut(s) 401
EcoRV GATATC 1 cut(s) 183
FaeI CATG 7 cut(s) 38, 86, 337, 346, 668, 706, 839
FaqI GGGAC 3 cut(s) 517, 546, 792
FatI CATG 7 cut(s) 34, 82, 333, 342, 664, 702, 835
FauI CCCGC 1 cut(s) 880
Fnu4HI GCNGC 5 cut(s) 513, 753, 842, 876, 879
FokI GGATG 3 cut(s) 185, 376, 533
Fsp4HI GCNGC 5 cut(s) 513, 753, 842, 876, 879
FspBI CTAG 3 cut(s) 60, 186, 338
GlaI GCGC 1 cut(s) 681
GluI GCNGC 5 cut(s) 513, 753, 842, 876, 879
GsuI CTGGAG 1 cut(s) 770
HaeIII GGCC 7 cut(s) 33, 396, 411, 755, 819, 930, 961
HgaI GACGC 4 cut(s) 562, 671, 786, 849
HhaI GCGC 1 cut(s) 682
Hin1I GRCGYC 2 cut(s) 797, 860
Hin1II CATG 7 cut(s) 38, 86, 337, 346, 668, 706, 839
Hin6I GCGC 1 cut(s) 680
HinP1I GCGC 1 cut(s) 680
HinfI GANTC 5 cut(s) 122, 645, 829, 943, 981
HphI GGTGA 2 cut(s) 131, 568
Hpy166II GTNNAC 1 cut(s) 700
Hpy188I TCNGA 5 cut(s) 127, 331, 569, 652, 722
Hpy188III TCNNGA 6 cut(s) 100, 343, 362, 550, 665, 826
Hpy8I GTNNAC 1 cut(s) 700
Hpy99I CGWCG 1 cut(s) 687
HpyAV CCTTC 5 cut(s) 102, 359, 449, 671, 977
HpyCH4III ACNGT 1 cut(s) 272
HpyCH4IV ACGT 2 cut(s) 284, 638
HpyCH4V TGCA 2 cut(s) 512, 955
HpyF10VI GCNNNNNNNGC 2 cut(s) 872, 887
HpyF3I CTNAG 3 cut(s) 206, 448, 971
HpySE526I ACGT 2 cut(s) 284, 638
Hsp92I GRCGYC 2 cut(s) 797, 860
Hsp92II CATG 7 cut(s) 38, 86, 337, 346, 668, 706, 839
HspAI GCGC 1 cut(s) 680
Kpn2I TCCGGA 1 cut(s) 825
Kzo9I GATC 6 cut(s) 427, 523, 552, 893, 935, 963
Lsp1109I GCAGC 2 cut(s) 524, 865
LweI GCATC 3 cut(s) 307, 560, 586
MaeI CTAG 3 cut(s) 60, 186, 338
MaeII ACGT 2 cut(s) 284, 638
MaeIII GTNAC 4 cut(s) 55, 146, 188, 574
MalI GATC 6 cut(s) 429, 525, 554, 895, 937, 965
MboI GATC 6 cut(s) 427, 523, 552, 893, 935, 963
MboII GAAGA 4 cut(s) 230, 649, 910, 981
MflI RGATCY 1 cut(s) 935
MluCI AATT 6 cut(s) 24, 70, 142, 606, 628, 723
MlyI GAGTC 1 cut(s) 823
MmeI TCCRAC 1 cut(s) 354
MroI TCCGGA 1 cut(s) 825
MroXI GAANNNNTTC 1 cut(s) 495
MseI TTAA 3 cut(s) 23, 27, 45
MslI CAYNNNNRTG 1 cut(s) 910
MspA1I CMGCKG 1 cut(s) 878
MspR9I CCNGG 7 cut(s) 403, 580, 602, 775, 810, 816, 927
MvaI CCWGG 1 cut(s) 403
MvnI CGCG 2 cut(s) 682, 752
MwoI GCNNNNNNNGC 2 cut(s) 872, 887
NciI CCSGG 6 cut(s) 580, 602, 775, 810, 816, 927
NdeII GATC 6 cut(s) 427, 523, 552, 893, 935, 963
NlaIII CATG 7 cut(s) 38, 86, 337, 346, 668, 706, 839
NlaIV GGNNCC 2 cut(s) 808, 814
NmuCI GTSAC 2 cut(s) 55, 574
NspI RCATGY 2 cut(s) 337, 706
OliI CACNNNNGTG 1 cut(s) 910
PacI TTAATTAA 1 cut(s) 27
PagI TCATGA 2 cut(s) 342, 664
PciI ACATGT 1 cut(s) 702
PcsI WCGNNNNNNNCGW 1 cut(s) 561
PdmI GAANNNNTTC 1 cut(s) 495
PfeI GAWTC 4 cut(s) 122, 645, 943, 981
PflMI CCANNNNNTGG 1 cut(s) 157
PfoI TCCNGGA 1 cut(s) 808
PkrI GCNGC 5 cut(s) 514, 754, 843, 877, 880
Ple19I CGATCG 1 cut(s) 555
PleI GAGTC 1 cut(s) 823
PpsI GAGTC 1 cut(s) 823
PscI ACATGT 1 cut(s) 702
PshBI ATTAAT 1 cut(s) 23
PsiI TTATAA 1 cut(s) 66
Psp1406I AACGTT 1 cut(s) 284
Psp6I CCWGG 1 cut(s) 401
PspGI CCWGG 1 cut(s) 401
PspN4I GGNNCC 2 cut(s) 808, 814
PspPI GGNCC 5 cut(s) 31, 409, 806, 812, 959
PsuI RGATCY 1 cut(s) 935
PvuI CGATCG 1 cut(s) 555
RsaI GTAC 2 cut(s) 239, 250
RsaNI GTAC 2 cut(s) 238, 249
RseI CAYNNNNRTG 1 cut(s) 910
SaqAI TTAA 3 cut(s) 23, 27, 45
SatI GCNGC 5 cut(s) 513, 753, 842, 876, 879
Sau3AI GATC 6 cut(s) 427, 523, 552, 893, 935, 963
Sau96I GGNCC 5 cut(s) 31, 409, 806, 812, 959
SchI GAGTC 1 cut(s) 823
ScrFI CCNGG 7 cut(s) 403, 580, 602, 775, 810, 816, 927
SfaNI GCATC 3 cut(s) 307, 560, 586
SinI GGWCC 2 cut(s) 806, 812
SmiMI CAYNNNNRTG 1 cut(s) 910
SpeI ACTAGT 1 cut(s) 59
Sse9I AATT 6 cut(s) 24, 70, 142, 606, 628, 723
SsiI CCGC 5 cut(s) 377, 752, 842, 873, 876
SspMI CTAG 3 cut(s) 60, 186, 338
StyD4I CCNGG 7 cut(s) 401, 578, 600, 773, 808, 814, 925
TaaI ACNGT 1 cut(s) 272
TaiI ACGT 2 cut(s) 287, 641
TaqI TCGA 4 cut(s) 194, 685, 765, 896
TasI AATT 6 cut(s) 24, 70, 142, 606, 628, 723
TatI WGTACW 1 cut(s) 248
TauI GCSGC 3 cut(s) 755, 844, 878
TfiI GAWTC 4 cut(s) 122, 645, 943, 981
Tru1I TTAA 3 cut(s) 23, 27, 45
Tru9I TTAA 3 cut(s) 23, 27, 45
TseFI GTSAC 2 cut(s) 55, 574
TseI GCWGC 2 cut(s) 512, 878
Tsp45I GTSAC 2 cut(s) 55, 574
TspDTI ATGAA 3 cut(s) 155, 653, 681
TspGWI ACGGA 1 cut(s) 321
Van91I CCANNNNNTGG 1 cut(s) 157
VpaK11BI GGWCC 2 cut(s) 806, 812
VspI ATTAAT 1 cut(s) 23
XapI RAATTY 3 cut(s) 606, 628, 723
XceI RCATGY 2 cut(s) 337, 706
XcmI CCANNNNNNNNNTGG 1 cut(s) 853
XmnI GAANNNNTTC 1 cut(s) 495
XspI CTAG 3 cut(s) 60, 186, 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.