Rmu_sc0000317.1_g000025

cationic amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000317.1
OG0018160
Physical Location & Seq
Reverse (-)
157577 .. 159016
1440 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1215 bp
atggattcccaaccgcaactggagcagcaagacccaaaacccagaagctactggaaaagatggaccaagcaggacttcttcccgaaacccactttccagaacttgagttcctccaaagacgccctctcccacaccccatcttacctcaaggactgcctcctcaaccgcttctccgactccttcaagctcctccacctccccaagcaaagcgagcaccacatgcatcgctgcctcacctggcgggaccacatctggctcggctttggctccatcgtcggctccggaattttcttcatcgccggagaggccactcttctagccgaacccgccattgtcttgtcctacaccatctccagcctctcggccctgctctctgtcctctgctacacagaatttgtcgtcgaagttactgttactggaggatctttctcatatcttctgatagaattgggagacttctttgcattcattgctgctgggaacattctcctccaggccatggtgggcgcggctgggttgcgcccgtcatggacttcttacttggttagcatgatcaacaccgacaacatagactttttgaggtttcaggtaaactctctgcctgatgggttcaatctgttggaccctattgctattgctgtgcttctggttgctaatggtatagcaatgagtgggacggctcggacttcgatgcaaaattggaaaataattagactcatagagcaaattctttgtcataccccggaaaattgtatttatttttcgaggattttccggaatttaaattggggttattggacggtttcgtggctcgtggatggagcggaagtgtttcggacgaatttttattcggaaaatatgactttaggggtggcgctaaagttgacttttgatacgttgagattcttcaaaaactttcttcacgaaagttatagagcgcgtcgatacgagttcgtggacaaatggaatgtgtcattcggagctcatcagatcgtctcctccgtctggtcgtccctctggtgtcctctagctgcgattctcctccatggaggcgctgcaagtctgcaaagtttggtgtcctctagctgcgattctcctccatggcggcactgcaagtctacaaagtttggtgttttcagacccgaccggaattccaagctcctgcgacggcacggcttcaagcttccatctttggtttcgtgggagactccttga

Protein Analysis

404

Amino Acids

45.94

Weight (kDa)

8.84

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_permease_2 PF13520 78 - 228 1.2e-06 Amino acid permease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 499
AccBSI CCGCTC 1 cut(s) 824
AccI GTMKAC 1 cut(s) 1118
AccII CGCG 2 cut(s) 509, 940
AccIII TCCGGA 2 cut(s) 281, 774
AciI CCGC 7 cut(s) 14, 166, 241, 327, 509, 824, 1105
AclWI GGATC 1 cut(s) 430
AcsI RAATTY 6 cut(s) 285, 392, 726, 778, 841, 1150
AcyI GRCGYC 1 cut(s) 120
AfiI CCNNNNNNNGG 2 cut(s) 499, 1005
AgsI TTSAA 4 cut(s) 184, 613, 910, 1180
AjnI CCWGG 2 cut(s) 236, 492
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AloI GAACNNNNNNTCC 2 cut(s) 92, 124
AluBI AGCT 7 cut(s) 48, 187, 983, 1031, 1086, 1159, 1183
AluI AGCT 7 cut(s) 48, 187, 983, 1031, 1086, 1159, 1183
Alw21I GWGCWC 2 cut(s) 216, 985
Alw26I GTCTC 3 cut(s) 447, 1000, 1199
AlwI GGATC 1 cut(s) 430
Aor13HI TCCGGA 2 cut(s) 281, 774
AoxI GGCC 3 cut(s) 306, 363, 495
ApeKI GCWGC 6 cut(s) 25, 228, 473, 1031, 1055, 1086
ApoI RAATTY 6 cut(s) 285, 392, 726, 778, 841, 1150
ArsI GACNNNNNNTTYG 2 cut(s) 718, 750
Asp700I GAANNNNTTC 1 cut(s) 831
AspLEI GCGC 5 cut(s) 509, 522, 877, 940, 1055
AspS9I GGNCC 4 cut(s) 63, 244, 364, 622
AsuC2I CCSGG 1 cut(s) 743
AsuHPI GGTGA 1 cut(s) 226
AvaII GGWCC 3 cut(s) 63, 244, 622
BanII GRGCYC 1 cut(s) 985
BauI CACGAG 1 cut(s) 812
Bbv12I GWGCWC 2 cut(s) 216, 985
BbvI GCAGC 6 cut(s) 37, 215, 460, 1018, 1042, 1073
BccI CCATC 7 cut(s) 54, 145, 278, 356, 599, 812, 1195
BceAI ACGGC 3 cut(s) 693, 1184, 1189
BciT130I CCWGG 2 cut(s) 238, 494
BclI TGATCA 1 cut(s) 552
BcnI CCSGG 1 cut(s) 743
BcoDI GTCTC 3 cut(s) 447, 1000, 1199
BfaI CTAG 3 cut(s) 317, 1028, 1083
BfoI RGCGCY 2 cut(s) 878, 1056
BglI GCCNNNNNGGC 1 cut(s) 305
BisI GCNGC 8 cut(s) 26, 229, 474, 510, 1032, 1056, 1087, 1106
BlsI GCNGC 8 cut(s) 27, 230, 475, 511, 1033, 1057, 1088, 1107
Bme1390I CCNGG 3 cut(s) 238, 494, 743
Bme18I GGWCC 3 cut(s) 63, 244, 622
BmgT120I GGNCC 4 cut(s) 63, 244, 364, 622
BmiI GGNNCC 4 cut(s) 245, 268, 280, 624
BmrFI CCNGG 3 cut(s) 238, 494, 743
BmsI GCATC 2 cut(s) 232, 681
BpmI CTGGAG 4 cut(s) 41, 337, 438, 476
BpuEI CTTGAG 2 cut(s) 124, 131
BpuMI CCSGG 1 cut(s) 743
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 4 cut(s) 498, 741, 1045, 1100
BsaWI WCCGGW 3 cut(s) 281, 774, 1146
BsaXI ACNNNNNCTCC 8 cut(s) 110, 140, 155, 185, 335, 365, 813, 843
Bsc4I CCNNNNNNNGG 2 cut(s) 499, 1005
Bse1I ACTGG 3 cut(s) 24, 56, 421
Bse3DI GCAATG 2 cut(s) 468, 672
BseAI TCCGGA 2 cut(s) 281, 774
BseBI CCWGG 2 cut(s) 238, 494
BseDI CCNNGG 4 cut(s) 498, 741, 1045, 1100
BseGI GGATG 1 cut(s) 823
BseLI CCNNNNNNNGG 2 cut(s) 499, 1005
BseMI GCAATG 2 cut(s) 468, 672
BseNI ACTGG 3 cut(s) 24, 56, 421
BseRI GAGGAG 6 cut(s) 149, 179, 479, 988, 1031, 1086
BseXI GCAGC 6 cut(s) 37, 215, 460, 1018, 1042, 1073
BseYI CCCAGC 2 cut(s) 476, 512
Bsh1236I CGCG 2 cut(s) 509, 940
Bsh1285I CGRYCG 1 cut(s) 1147
BshFI GGCC 3 cut(s) 308, 365, 497
BsiEI CGRYCG 1 cut(s) 1147
BsiHKAI GWGCWC 2 cut(s) 216, 985
BsiSI CCGG 5 cut(s) 282, 300, 743, 775, 1147
BslFI GGGAC 3 cut(s) 257, 688, 997
BslI CCNNNNNNNGG 2 cut(s) 499, 1005
BsmAI GTCTC 3 cut(s) 447, 1000, 1199
BsmBI CGTCTC 1 cut(s) 1000
BsmFI GGGAC 3 cut(s) 257, 688, 997
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 3 cut(s) 308, 365, 497
Bsp1286I GDGCHC 2 cut(s) 216, 985
Bsp13I TCCGGA 2 cut(s) 281, 774
Bsp143I GATC 3 cut(s) 422, 552, 990
Bsp19I CCATGG 3 cut(s) 498, 1045, 1100
BspACI CCGC 7 cut(s) 14, 166, 241, 327, 509, 824, 1105
BspANI GGCC 3 cut(s) 308, 365, 497
BspEI TCCGGA 2 cut(s) 281, 774
BspFNI CGCG 2 cut(s) 509, 940
BspLI GGNNCC 4 cut(s) 245, 268, 280, 624
BspPI GGATC 1 cut(s) 430
BsrBI CCGCTC 1 cut(s) 824
BsrDI GCAATG 2 cut(s) 468, 672
BsrI ACTGG 3 cut(s) 24, 56, 421
BssECI CCNNGG 4 cut(s) 498, 741, 1045, 1100
BssMI GATC 3 cut(s) 422, 552, 990
BssNI GRCGYC 1 cut(s) 120
BssSI CACGAG 1 cut(s) 812
BssT1I CCWWGG 3 cut(s) 498, 1045, 1100
Bst2BI CACGAG 1 cut(s) 812
Bst2UI CCWGG 2 cut(s) 238, 494
Bst4CI ACNGT 2 cut(s) 412, 802
Bst6I CTCTTC 1 cut(s) 318
BstACI GRCGYC 1 cut(s) 120
BstAPI GCANNNNNTGC 2 cut(s) 220, 470
BstC8I GCNNGC 1 cut(s) 212
BstDSI CCRYGG 3 cut(s) 498, 1045, 1100
BstF5I GGATG 1 cut(s) 823
BstFNI CGCG 2 cut(s) 509, 940
BstH2I RGCGCY 2 cut(s) 878, 1056
BstHHI GCGC 5 cut(s) 509, 522, 877, 940, 1055
BstKTI GATC 3 cut(s) 425, 555, 993
BstMAI GTCTC 3 cut(s) 447, 1000, 1199
BstMBI GATC 3 cut(s) 422, 552, 990
BstMCI CGRYCG 1 cut(s) 1147
BstMWI GCNNNNNNNGC 6 cut(s) 22, 211, 220, 305, 326, 470
BstNI CCWGG 2 cut(s) 238, 494
BstNSI RCATGY 1 cut(s) 223
BstSCI CCNGG 3 cut(s) 236, 492, 741
BstUI CGCG 2 cut(s) 509, 940
BstV1I GCAGC 6 cut(s) 37, 215, 460, 1018, 1042, 1073
BstX2I RGATCY 1 cut(s) 422
BstYI RGATCY 1 cut(s) 422
BsuRI GGCC 3 cut(s) 308, 365, 497
BtgI CCRYGG 3 cut(s) 498, 1045, 1100
BtgZI GCGATG 2 cut(s) 209, 280
BtsCI GGATG 1 cut(s) 823
BtsI GCAGTG 1 cut(s) 1108
BtsIMutI CAGTG 1 cut(s) 1108
Cac8I GCNNGC 1 cut(s) 212
CfoI GCGC 5 cut(s) 509, 522, 877, 940, 1055
Cfr13I GGNCC 4 cut(s) 63, 244, 364, 622
CseI GACGC 2 cut(s) 128, 929
CviAII CATG 6 cut(s) 220, 499, 528, 550, 1046, 1101
DpnI GATC 3 cut(s) 424, 554, 992
DpnII GATC 3 cut(s) 422, 552, 990
DraI TTTAAA 1 cut(s) 783
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Ecl136II GAGCTC 1 cut(s) 983
Eco130I CCWWGG 3 cut(s) 498, 1045, 1100
Eco24I GRGCYC 1 cut(s) 985
Eco47I GGWCC 3 cut(s) 63, 244, 622
Eco53kI GAGCTC 1 cut(s) 983
EcoICRI GAGCTC 1 cut(s) 983
EcoRI GAATTC 1 cut(s) 1150
EcoRII CCWGG 2 cut(s) 236, 492
EcoT14I CCWWGG 3 cut(s) 498, 1045, 1100
EcoT22I ATGCAT 1 cut(s) 225
EcoT38I GRGCYC 1 cut(s) 985
ErhI CCWWGG 3 cut(s) 498, 1045, 1100
Esp3I CGTCTC 1 cut(s) 1000
FaeI CATG 6 cut(s) 223, 502, 531, 553, 1049, 1104
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FaqI GGGAC 3 cut(s) 257, 688, 997
FatI CATG 6 cut(s) 219, 498, 527, 549, 1045, 1100
FauI CCCGC 2 cut(s) 234, 334
FbaI TGATCA 1 cut(s) 552
FblI GTMKAC 1 cut(s) 1118
Fnu4HI GCNGC 8 cut(s) 26, 229, 474, 510, 1032, 1056, 1087, 1106
FokI GGATG 1 cut(s) 830
FriOI GRGCYC 1 cut(s) 985
Fsp4HI GCNGC 8 cut(s) 26, 229, 474, 510, 1032, 1056, 1087, 1106
FspBI CTAG 3 cut(s) 317, 1028, 1083
GlaI GCGC 5 cut(s) 508, 521, 876, 939, 1054
GluI GCNGC 8 cut(s) 26, 229, 474, 510, 1032, 1056, 1087, 1106
GsaI CCCAGC 2 cut(s) 480, 516
GsuI CTGGAG 4 cut(s) 41, 337, 438, 476
HaeII RGCGCY 2 cut(s) 878, 1056
HaeIII GGCC 3 cut(s) 308, 365, 497
HapII CCGG 5 cut(s) 282, 300, 743, 775, 1147
HgaI GACGC 2 cut(s) 128, 929
HhaI GCGC 5 cut(s) 509, 522, 877, 940, 1055
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 6 cut(s) 223, 502, 531, 553, 1049, 1104
Hin6I GCGC 5 cut(s) 507, 520, 875, 938, 1053
HinP1I GCGC 5 cut(s) 507, 520, 875, 938, 1053
HincII GTYRAC 1 cut(s) 885
HindII GTYRAC 1 cut(s) 885
HindIII AAGCTT 1 cut(s) 1181
HinfI GANTC 7 cut(s) 5, 176, 714, 903, 1036, 1091, 1207
HpaII CCGG 5 cut(s) 282, 300, 743, 775, 1147
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 4 cut(s) 592, 885, 958, 1119
Hpy188I TCNGA 8 cut(s) 175, 441, 684, 837, 853, 980, 990, 1139
Hpy188III TCNNGA 5 cut(s) 82, 97, 282, 775, 923
Hpy8I GTNNAC 4 cut(s) 592, 885, 958, 1119
Hpy99I CGWCG 4 cut(s) 278, 404, 945, 1170
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 2 cut(s) 412, 802
HpyCH4IV ACGT 1 cut(s) 896
HpyCH4V TGCA 6 cut(s) 223, 464, 694, 1058, 1066, 1113
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 211, 220, 305, 326, 470
HpySE526I ACGT 1 cut(s) 896
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 6 cut(s) 223, 502, 531, 553, 1049, 1104
HspAI GCGC 5 cut(s) 507, 520, 875, 938, 1053
Kpn2I TCCGGA 2 cut(s) 281, 774
Ksp22I TGATCA 1 cut(s) 552
Kzo9I GATC 3 cut(s) 422, 552, 990
LmnI GCTCC 7 cut(s) 22, 192, 272, 284, 821, 980, 1164
Lsp1109I GCAGC 6 cut(s) 37, 215, 460, 1018, 1042, 1073
LweI GCATC 2 cut(s) 232, 681
MaeI CTAG 3 cut(s) 317, 1028, 1083
MaeII ACGT 1 cut(s) 896
MaeIII GTNAC 2 cut(s) 406, 412
MalI GATC 3 cut(s) 424, 554, 992
MbiI CCGCTC 1 cut(s) 824
MboI GATC 3 cut(s) 422, 552, 990
MboII GAAGA 6 cut(s) 70, 283, 305, 428, 898, 911
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 2 cut(s) 216, 985
MlyI GAGTC 3 cut(s) 170, 708, 1201
MmeI TCCRAC 2 cut(s) 198, 600
Mph1103I ATGCAT 1 cut(s) 225
MroI TCCGGA 2 cut(s) 281, 774
MroXI GAANNNNTTC 1 cut(s) 831
MseI TTAA 1 cut(s) 782
MspI CCGG 5 cut(s) 282, 300, 743, 775, 1147
MspR9I CCNGG 3 cut(s) 238, 494, 743
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 2 cut(s) 238, 494
MvnI CGCG 2 cut(s) 509, 940
MwoI GCNNNNNNNGC 6 cut(s) 22, 211, 220, 305, 326, 470
NciI CCSGG 1 cut(s) 743
NcoI CCATGG 3 cut(s) 498, 1045, 1100
NdeII GATC 3 cut(s) 422, 552, 990
NlaIII CATG 6 cut(s) 223, 502, 531, 553, 1049, 1104
NlaIV GGNNCC 4 cut(s) 245, 268, 280, 624
NmeAIII GCCGAG 2 cut(s) 237, 341
NsiI ATGCAT 1 cut(s) 225
NspI RCATGY 1 cut(s) 223
PcsI WCGNNNNNNNCGW 1 cut(s) 999
PctI GAATGC 1 cut(s) 464
PdmI GAANNNNTTC 1 cut(s) 831
PfeI GAWTC 4 cut(s) 5, 903, 1036, 1091
PflMI CCANNNNNTGG 1 cut(s) 499
PkrI GCNGC 8 cut(s) 27, 230, 475, 511, 1033, 1057, 1088, 1107
PleI GAGTC 3 cut(s) 170, 708, 1201
PpsI GAGTC 3 cut(s) 170, 708, 1201
Psp124BI GAGCTC 1 cut(s) 985
Psp6I CCWGG 2 cut(s) 236, 492
PspFI CCCAGC 2 cut(s) 476, 512
PspGI CCWGG 2 cut(s) 236, 492
PspN4I GGNNCC 4 cut(s) 245, 268, 280, 624
PspPI GGNCC 4 cut(s) 63, 244, 364, 622
PsuI RGATCY 1 cut(s) 422
SacI GAGCTC 1 cut(s) 985
SaqAI TTAA 1 cut(s) 782
SatI GCNGC 8 cut(s) 26, 229, 474, 510, 1032, 1056, 1087, 1106
Sau3AI GATC 3 cut(s) 422, 552, 990
Sau96I GGNCC 4 cut(s) 63, 244, 364, 622
SchI GAGTC 3 cut(s) 170, 708, 1201
ScrFI CCNGG 3 cut(s) 238, 494, 743
SduI GDGCHC 2 cut(s) 216, 985
SfaNI GCATC 2 cut(s) 232, 681
SinI GGWCC 3 cut(s) 63, 244, 622
SmiI ATTTAAAT 1 cut(s) 783
SmlI CTYRAG 2 cut(s) 103, 146
SmoI CTYRAG 2 cut(s) 103, 146
SsiI CCGC 7 cut(s) 14, 166, 241, 327, 509, 824, 1105
SspMI CTAG 3 cut(s) 317, 1028, 1083
SstI GAGCTC 1 cut(s) 985
StyD4I CCNGG 3 cut(s) 236, 492, 741
StyI CCWWGG 3 cut(s) 498, 1045, 1100
SwaI ATTTAAAT 1 cut(s) 783
TaaI ACNGT 2 cut(s) 412, 802
TaiI ACGT 1 cut(s) 899
TaqI TCGA 4 cut(s) 402, 689, 764, 943
TauI GCSGC 2 cut(s) 512, 1108
TfiI GAWTC 4 cut(s) 5, 903, 1036, 1091
Tru1I TTAA 1 cut(s) 782
Tru9I TTAA 1 cut(s) 782
TscAI CASTG 1 cut(s) 1115
TseI GCWGC 6 cut(s) 25, 228, 473, 1031, 1055, 1086
TspDTI ATGAA 2 cut(s) 283, 457
TspGWI ACGGA 1 cut(s) 991
TspRI CASTG 1 cut(s) 1115
Van91I CCANNNNNTGG 1 cut(s) 499
VpaK11BI GGWCC 3 cut(s) 63, 244, 622
XapI RAATTY 6 cut(s) 285, 392, 726, 778, 841, 1150
XceI RCATGY 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 1118
XmnI GAANNNNTTC 1 cut(s) 831
XspI CTAG 3 cut(s) 317, 1028, 1083
Zsp2I ATGCAT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.