Rmu_sc0000333.1_g000029
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000333.1
OG0006137
Physical Location & Seq
Reverse (-)
221284 .. 226598
5315 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2886 bp
atgcagacaatatgcttcatgttgttgttggtagtaattgcagcatcagggcagtcagatttagatgcactcttagagctcaaaaaagggattcagaaagacccttctggccaagttcttgtttcatgggattcaaagtccttggcctctgatggatgccccctcaattggtttgggatagtttgtagtgatggccttgtcacatccatttctataaacgatattggcctggttggcgagttcagtttttcagccattgctggccttaaagtgctgcgcaatttatcactctcaaacaaccagctcacaggggctctctcaaaggtctctctgattcagtctctacagcatttagatctctcacgcaatttgtttcatgggttaataccatctggcttagcagacttaaagcatttagcgcttttgaatctgtcttcaaaccaatttgaaggcattttcccctctggttttgggaaacttgaacagctgaggtacattgatgttcgatataatgccttgtctggggatatcatgacttttctatcccaaatgggtagcgtggttcatgttgatctaagtagcaatctgttttctggttcactggatttggaaattgttagttggtcctatcggatacttcggctcggaagcaaccagctatcaggttctctaccggaagctctgttgcaaggaagctcaatgctcttgtctgaattggatcttagtcttaatcatcttgaaggtccagtaggaagcataacctctgcaactctgaagaagctgaatatatcttcaaataaattatcagggtccttgcctgccaatgttggccactgtgccatcgtagacctcagtaataatatgctcactggtaacctgtcccgaatccgtagttggggaaattacattgaagttatccaactaagttcaaattctttgacaggaagcttgcctggccaaacatcccaattttttaggctaacttctgttaagatatccaataactcattagagggtgttcttccatcagtattgggtacatacccagaactaaaagtgatagatcttagcctcaaccggttgcaaggttttctccttccaagctttttctcttcaacaatattgactgatctaaacttgtcaggcaacaatttatctgggtccatacctgtccaggaaattacaattggtcctattcaaaatttgagcctggtgtctctagatctgtcaaataattcattgagtggtcatttgccccaagaattcagcaagtttcatagcttggtatatctcaacctgtctgttaataattttgaaggtaggattcctgagaaccttccagatgaactgaaagagttcaacgtctctttgaataatttttctggtcttgtacctgaaaatttaaggcacttccctgtttcagcattttatccagggaattctttgctgatgtttcctcatcccctatcatcaccaaacaatgtccctgatatgatctctagaaatcacaggtctcctataaaagctgctattagaattgccctgattgtaggtctgcttggtggtggtgctattgtagccctctcatgcatgatgatctattatagggcccactgtcaaggatgtagaaagagaggtcaaaaagcaagctgtgaaaagaatggtgttgtgcaaggggactcttccctttctcatagatcagtacctggcaaaactgaagacttaaaatcttcatatggtttccttcaagacccttcgccatcatccgcaagggagtctactcataatgccggtgacacttcatcagttctagagaagtctaaacaattgggtcatcctgaatcaacaaagctagaggaaggggtatcttctcccatgtctctcttatcgccttcaaacccattgccttcgaaaagccaagaaccactcaatagttctgctgttctcaacacttgttctccagacaaactagctggtgatttacatctgtttgatggctccctggcattcacagcagaagaactttcatgtgctcccgcagaagctattggaaggagttgtcatggaacaatgtacaaagcaatgctcgagtctggtcatgtaattgctgtcaaatggttaagggagggaatagcaaaaggaaggaaagaatttgctagagaagtaaagaaacttgggagcatccgccatccaaatctagtttctttgcagggttactattggggcccaaaggagcatgagaaacttattatatcaagttatatcaatgctgaatctttggccttctatctccatgaggttgagccaagaaaactctcacccttgtctctcgaggaacggctcaaggtttctgtagatgttgcccgatgtctgaactacctccataatgagaaggcaattccccatggcaacctcaaatccacaaatattctactagaaactcctaatcataatgcactccttacagattatagtatccaccgaatattgactcctgctggaactactgagcaagttctgaatgcaggtgctcttggctatcggccccctgaatttgctagttcaagtagaccctgcccctcattgaagagtgatgtctatgcatttggagtgatcttgttggagctgctgactggaaagagttctggggagattgtctctggcataccaggtgtggttgatctgactgattgggtgagatttttggcagaagaaaaccgttcttttgaatgccttgacagactgattctggaaaagcccagtattaagcactcgcctagagttcttgatgaattgctccaggtagctttaagatgcatccttccagcatccgagagacccgacattaaaactgtgtttgaagacctttcaagaatagtgtactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase_Tyr

Protein Analysis

961

Amino Acids

104.35

Weight (kDa)

6.38

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 19 - 63 4.7e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 92 - 149 6.9e-06 Leucine rich repeat
LRR_8 PF13855 114 - 173 4.5e-10 Leucine rich repeat
LRR_8 PF13855 138 - 196 9.1e-07 Leucine rich repeat
LRR_8 PF13855 326 - 385 3.8e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 681 - 952 1.6e-26 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 681 - 881 5.1e-23 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2516
Acc16I TGCGCA 1 cut(s) 278
Acc36I ACCTGC 1 cut(s) 2516
AccI GTMKAC 3 cut(s) 846, 1778, 2569
AciI CCGC 3 cut(s) 1767, 2037, 2185
AclWI GGATC 1 cut(s) 726
AcoI YGGCCR 3 cut(s) 109, 829, 955
AcsI RAATTY 7 cut(s) 931, 1201, 1262, 1399, 1438, 2150, 2552
AcuI CTGAAG 2 cut(s) 794, 1737
AfaI GTAC 6 cut(s) 494, 1039, 1392, 1704, 2075, 2882
AfeI AGCGCT 1 cut(s) 420
AfiI CCNNNNNNNGG 3 cut(s) 168, 522, 895
AgeI ACCGGT 1 cut(s) 1077
AjnI CCWGG 9 cut(s) 228, 952, 1173, 1209, 1432, 1705, 2001, 2668, 2799
AjuI GAANNNNNNNTTGG 6 cut(s) 877, 909, 1170, 1202, 2185, 2217
Alw21I GWGCWC 3 cut(s) 81, 2035, 2533
Alw26I GTCTC 9 cut(s) 331, 345, 1221, 1369, 1518, 1884, 2331, 2662, 2830
AlwI GGATC 1 cut(s) 726
AlwNI CAGNNNCTG 1 cut(s) 1706
Ama87I CYCGRG 2 cut(s) 2087, 2330
Aor51HI AGCGCT 1 cut(s) 420
ApaI GGGCCC 2 cut(s) 1612, 2228
ApeKI GCWGC 4 cut(s) 41, 274, 1526, 2626
ApoI RAATTY 7 cut(s) 931, 1201, 1262, 1399, 1438, 2150, 2552
AsiGI ACCGGT 1 cut(s) 1077
Asp700I GAANNNNTTC 2 cut(s) 1355, 2641
AspLEI GCGC 2 cut(s) 279, 421
AsuHPI GGTGA 5 cut(s) 1464, 1805, 1988, 2310, 2707
AsuII TTCGAA 1 cut(s) 1910
AvaI CYCGRG 2 cut(s) 2087, 2330
AvaII GGWCC 5 cut(s) 624, 743, 810, 1161, 1190
BaeGI GKGCMC 2 cut(s) 1612, 2228
BalI TGGCCA 3 cut(s) 111, 831, 957
BanII GRGCYC 4 cut(s) 81, 316, 1612, 2228
BbsI GAAGAC 3 cut(s) 426, 1725, 2868
Bbv12I GWGCWC 3 cut(s) 81, 2035, 2533
BbvCI CCTCAGC 1 cut(s) 488
BbvI GCAGC 4 cut(s) 53, 261, 1513, 2613
BccI CCATC 8 cut(s) 146, 185, 397, 848, 1033, 1768, 1988, 2196
BceAI ACGGC 1 cut(s) 2354
BciT130I CCWGG 9 cut(s) 230, 954, 1175, 1211, 1434, 1707, 2003, 2670, 2801
BciVI GTATCC 2 cut(s) 627, 2486
BcoDI GTCTC 9 cut(s) 331, 345, 1221, 1369, 1518, 1884, 2331, 2662, 2830
BfmI CTRYAG 2 cut(s) 344, 2352
BfoI RGCGCY 1 cut(s) 422
BfuAI ACCTGC 1 cut(s) 2516
BfuI GTATCC 2 cut(s) 627, 2486
BglI GCCNNNNNGGC 1 cut(s) 234
BglII AGATCT 3 cut(s) 355, 1063, 1222
BisI GCNGC 4 cut(s) 42, 275, 1527, 2627
BlpI GCTNAGC 1 cut(s) 397
BlsI GCNGC 4 cut(s) 43, 276, 1528, 2628
Bme1390I CCNGG 9 cut(s) 230, 954, 1175, 1211, 1434, 1707, 2003, 2670, 2801
Bme18I GGWCC 5 cut(s) 624, 743, 810, 1161, 1190
BmeT110I CYCGRG 2 cut(s) 2087, 2330
BmiI GGNNCC 7 cut(s) 811, 1162, 1610, 1999, 2225, 2226, 2546
BmrFI CCNGG 9 cut(s) 230, 954, 1175, 1211, 1434, 1707, 2003, 2670, 2801
BmrI ACTGGG 1 cut(s) 2754
BmsI GCATC 7 cut(s) 53, 55, 146, 2190, 2804, 2826, 2837
BmuI ACTGGG 1 cut(s) 2754
BpiI GAAGAC 3 cut(s) 426, 1725, 2868
BplI GAGNNNNNCTC 2 cut(s) 2642, 2674
BpmI CTGGAG 2 cut(s) 1944, 2783
Bpu10I CCTNAGC 1 cut(s) 488
Bpu1102I GCTNAGC 1 cut(s) 397
Bpu14I TTCGAA 1 cut(s) 1910
BpuEI CTTGAG 1 cut(s) 2327
BsaBI GATNNNNATC 1 cut(s) 1983
BsaI GGTCTC 3 cut(s) 331, 1518, 2830
BsaJI CCNNGG 4 cut(s) 141, 1433, 2001, 2404
BsaWI WCCGGW 2 cut(s) 673, 1077
BsaXI ACNNNNNCTCC 2 cut(s) 1942, 1972
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 522, 895
Bse118I RCCGGY 2 cut(s) 1077, 1790
Bse1I ACTGG 5 cut(s) 606, 746, 874, 2638, 2760
Bse3DI GCAATG 3 cut(s) 255, 1901, 2088
Bse8I GATNNNNATC 1 cut(s) 1983
BseBI CCWGG 9 cut(s) 230, 954, 1175, 1211, 1434, 1707, 2003, 2670, 2801
BseDI CCNNGG 4 cut(s) 141, 1433, 2001, 2404
BseJI GATNNNNATC 1 cut(s) 1983
BseLI CCNNNNNNNGG 3 cut(s) 168, 522, 895
BseMI GCAATG 3 cut(s) 255, 1901, 2088
BseMII CTCAG 4 cut(s) 479, 865, 1320, 2499
BseNI ACTGG 5 cut(s) 606, 746, 874, 2638, 2760
BseSI GKGCMC 2 cut(s) 1612, 2228
BseXI GCAGC 4 cut(s) 53, 261, 1513, 2613
BshTI ACCGGT 1 cut(s) 1077
BsiHKAI GWGCWC 3 cut(s) 81, 2035, 2533
BsiHKCI CYCGRG 2 cut(s) 2087, 2330
BsiSI CCGG 3 cut(s) 674, 1078, 1791
BslFI GGGAC 3 cut(s) 865, 1469, 1691
BslI CCNNNNNNNGG 3 cut(s) 168, 522, 895
BsmAI GTCTC 9 cut(s) 331, 345, 1221, 1369, 1518, 1884, 2331, 2662, 2830
BsmBI CGTCTC 1 cut(s) 1369
BsmFI GGGAC 3 cut(s) 865, 1469, 1691
BsmI GAATGC 3 cut(s) 2006, 2527, 2735
Bso31I GGTCTC 3 cut(s) 331, 1518, 2830
BsoBI CYCGRG 2 cut(s) 2087, 2330
Bsp119I TTCGAA 1 cut(s) 1910
Bsp120I GGGCCC 2 cut(s) 1608, 2224
Bsp1286I GDGCHC 6 cut(s) 81, 316, 1612, 2035, 2228, 2533
Bsp1407I TGTACA 1 cut(s) 2073
Bsp1720I GCTNAGC 1 cut(s) 397
Bsp19I CCATGG 1 cut(s) 2404
BspACI CCGC 3 cut(s) 1767, 2037, 2185
BspCNI CTCAG 4 cut(s) 480, 864, 1321, 2500
BspHI TCATGA 1 cut(s) 531
BspLI GGNNCC 7 cut(s) 811, 1162, 1610, 1999, 2225, 2226, 2546
BspMI ACCTGC 1 cut(s) 2516
BspPI GGATC 1 cut(s) 726
BspT104I TTCGAA 1 cut(s) 1910
BspTNI GGTCTC 3 cut(s) 331, 1518, 2830
BsrDI GCAATG 3 cut(s) 255, 1901, 2088
BsrFI RCCGGY 2 cut(s) 1077, 1790
BsrGI TGTACA 1 cut(s) 2073
BsrI ACTGG 5 cut(s) 606, 746, 874, 2638, 2760
BssAI RCCGGY 2 cut(s) 1077, 1790
BssECI CCNNGG 4 cut(s) 141, 1433, 2001, 2404
BssT1I CCWWGG 2 cut(s) 141, 2404
Bst2UI CCWGG 9 cut(s) 230, 954, 1175, 1211, 1434, 1707, 2003, 2670, 2801
Bst4CI ACNGT 4 cut(s) 836, 1616, 2720, 2854
Bst6I CTCTTC 3 cut(s) 1117, 1687, 2582
BstAUI TGTACA 1 cut(s) 2073
BstBI TTCGAA 1 cut(s) 1910
BstC8I GCNNGC 4 cut(s) 262, 819, 950, 1648
BstDSI CCRYGG 1 cut(s) 2404
BstEII GGTNACC 1 cut(s) 872
BstH2I RGCGCY 1 cut(s) 422
BstHHI GCGC 2 cut(s) 279, 421
BstMAI GTCTC 9 cut(s) 331, 345, 1221, 1369, 1518, 1884, 2331, 2662, 2830
BstMWI GCNNNNNNNGC 5 cut(s) 234, 418, 954, 1577, 2012
BstNI CCWGG 9 cut(s) 230, 954, 1175, 1211, 1434, 1707, 2003, 2670, 2801
BstPI GGTNACC 1 cut(s) 872
BstSCI CCNGG 9 cut(s) 228, 952, 1173, 1209, 1432, 1705, 2001, 2668, 2799
BstSFI CTRYAG 2 cut(s) 344, 2352
BstSLI GKGCMC 2 cut(s) 1612, 2228
BstV1I GCAGC 4 cut(s) 53, 261, 1513, 2613
BstV2I GAAGAC 3 cut(s) 426, 1725, 2868
BstX2I RGATCY 4 cut(s) 355, 718, 1063, 1222
BstYI RGATCY 4 cut(s) 355, 718, 1063, 1222
BsuI GTATCC 2 cut(s) 627, 2486
BtgI CCRYGG 1 cut(s) 2404
BtsIMutI CAGTG 4 cut(s) 599, 832, 867, 1612
BveI ACCTGC 1 cut(s) 2516
Cac8I GCNNGC 4 cut(s) 262, 819, 950, 1648
CaiI CAGNNNCTG 1 cut(s) 1706
CciI TCATGA 1 cut(s) 531
CfoI GCGC 2 cut(s) 279, 421
Cfr10I RCCGGY 2 cut(s) 1077, 1790
CsiI ACCWGGT 1 cut(s) 2668
Csp6I GTAC 6 cut(s) 493, 1038, 1391, 1703, 2074, 2881
CspAI ACCGGT 1 cut(s) 1077
CviQI GTAC 6 cut(s) 493, 1038, 1391, 1703, 2074, 2881
EaeI YGGCCR 3 cut(s) 109, 829, 955
Eam1104I CTCTTC 3 cut(s) 1117, 1687, 2582
EarI CTCTTC 3 cut(s) 1117, 1687, 2582
EciI GGCGGA 1 cut(s) 2174
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 2 cut(s) 141, 2404
Eco24I GRGCYC 4 cut(s) 81, 316, 1612, 2228
Eco31I GGTCTC 3 cut(s) 331, 1518, 2830
Eco32I GATATC 2 cut(s) 529, 996
Eco47I GGWCC 5 cut(s) 624, 743, 810, 1161, 1190
Eco47III AGCGCT 1 cut(s) 420
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 2 cut(s) 794, 1737
Eco88I CYCGRG 2 cut(s) 2087, 2330
Eco91I GGTNACC 1 cut(s) 872
EcoICRI GAGCTC 1 cut(s) 79
EcoO109I RGGNCCY 3 cut(s) 810, 1608, 2224
EcoO65I GGTNACC 1 cut(s) 872
EcoRI GAATTC 2 cut(s) 1262, 1438
EcoRII CCWGG 9 cut(s) 228, 952, 1173, 1209, 1432, 1705, 2001, 2668, 2799
EcoRV GATATC 2 cut(s) 529, 996
EcoT14I CCWWGG 2 cut(s) 141, 2404
EcoT22I ATGCAT 3 cut(s) 1592, 2605, 2819
EcoT38I GRGCYC 4 cut(s) 81, 316, 1612, 2228
ErhI CCWWGG 2 cut(s) 141, 2404
Esp3I CGTCTC 1 cut(s) 1369
FaqI GGGAC 3 cut(s) 865, 1469, 1691
FauI CCCGC 1 cut(s) 2044
FauNDI CATATG 1 cut(s) 1735
FblI GTMKAC 3 cut(s) 846, 1778, 2569
Fnu4HI GCNGC 4 cut(s) 42, 275, 1527, 2627
FriOI GRGCYC 4 cut(s) 81, 316, 1612, 2228
Fsp4HI GCNGC 4 cut(s) 42, 275, 1527, 2627
FspI TGCGCA 1 cut(s) 278
GlaI GCGC 2 cut(s) 278, 420
GluI GCNGC 4 cut(s) 42, 275, 1527, 2627
GsuI CTGGAG 2 cut(s) 1944, 2783
HaeII RGCGCY 1 cut(s) 422
HapII CCGG 3 cut(s) 674, 1078, 1791
HhaI GCGC 2 cut(s) 279, 421
Hin6I GCGC 2 cut(s) 277, 419
HinP1I GCGC 2 cut(s) 277, 419
HindIII AAGCTT 2 cut(s) 946, 1102
HpaII CCGG 3 cut(s) 674, 1078, 1791
HphI GGTGA 5 cut(s) 1464, 1805, 1988, 2310, 2707
Hpy166II GTNNAC 5 cut(s) 599, 847, 1779, 2570, 2881
Hpy8I GTNNAC 5 cut(s) 599, 847, 1779, 2570, 2881
HpyCH4III ACNGT 4 cut(s) 836, 1616, 2720, 2854
HpyCH4IV ACGT 1 cut(s) 1362
HpyF10VI GCNNNNNNNGC 5 cut(s) 234, 418, 954, 1577, 2012
HpySE526I ACGT 1 cut(s) 1362
HspAI GCGC 2 cut(s) 277, 419
LmnI GCTCC 6 cut(s) 2003, 2038, 2178, 2233, 2623, 2802
Lsp1109I GCAGC 4 cut(s) 53, 261, 1513, 2613
LweI GCATC 7 cut(s) 53, 55, 146, 2190, 2804, 2826, 2837
MabI ACCWGGT 1 cut(s) 2668
MaeII ACGT 1 cut(s) 1362
MaeIII GTNAC 4 cut(s) 199, 872, 1793, 2213
MfeI CAATTG 3 cut(s) 166, 1185, 1826
MflI RGATCY 4 cut(s) 355, 718, 1063, 1222
MhlI GDGCHC 6 cut(s) 81, 316, 1612, 2035, 2228, 2533
MlsI TGGCCA 3 cut(s) 111, 831, 957
MluNI TGGCCA 3 cut(s) 111, 831, 957
MlyI GAGTC 4 cut(s) 1673, 1784, 2099, 2485
MmeI TCCRAC 2 cut(s) 943, 2601
Mox20I TGGCCA 3 cut(s) 111, 831, 957
Mph1103I ATGCAT 3 cut(s) 1592, 2605, 2819
MroXI GAANNNNTTC 2 cut(s) 1355, 2641
MscI TGGCCA 3 cut(s) 111, 831, 957
Msp20I TGGCCA 3 cut(s) 111, 831, 957
MspA1I CMGCKG 1 cut(s) 487
MspI CCGG 3 cut(s) 674, 1078, 1791
MspR9I CCNGG 9 cut(s) 230, 954, 1175, 1211, 1434, 1707, 2003, 2670, 2801
MunI CAATTG 3 cut(s) 166, 1185, 1826
Mva1269I GAATGC 3 cut(s) 2006, 2527, 2735
MvaI CCWGG 9 cut(s) 230, 954, 1175, 1211, 1434, 1707, 2003, 2670, 2801
MwoI GCNNNNNNNGC 5 cut(s) 234, 418, 954, 1577, 2012
NcoI CCATGG 1 cut(s) 2404
NdeI CATATG 1 cut(s) 1735
NlaIV GGNNCC 7 cut(s) 811, 1162, 1610, 1999, 2225, 2226, 2546
NmuCI GTSAC 2 cut(s) 199, 1793
NsbI TGCGCA 1 cut(s) 278
NsiI ATGCAT 3 cut(s) 1592, 2605, 2819
NspV TTCGAA 1 cut(s) 1910
PaeR7I CTCGAG 2 cut(s) 2087, 2330
PagI TCATGA 1 cut(s) 531
PaqCI CACCTGC 1 cut(s) 2516
PctI GAATGC 3 cut(s) 2006, 2527, 2735
PdmI GAANNNNTTC 2 cut(s) 1355, 2641
PfeI GAWTC 9 cut(s) 91, 131, 334, 427, 885, 1324, 1841, 2273, 2746
PfoI TCCNGGA 1 cut(s) 1173
PinAI ACCGGT 1 cut(s) 1077
PkrI GCNGC 4 cut(s) 43, 276, 1528, 2628
PleI GAGTC 4 cut(s) 1673, 1783, 2098, 2485
PpsI GAGTC 4 cut(s) 1673, 1783, 2098, 2485
PpuMI RGGWCCY 1 cut(s) 810
Psp124BI GAGCTC 1 cut(s) 81
Psp5II RGGWCCY 1 cut(s) 810
Psp6I CCWGG 9 cut(s) 228, 952, 1173, 1209, 1432, 1705, 2001, 2668, 2799
PspEI GGTNACC 1 cut(s) 872
PspGI CCWGG 9 cut(s) 228, 952, 1173, 1209, 1432, 1705, 2001, 2668, 2799
PspN4I GGNNCC 7 cut(s) 811, 1162, 1610, 1999, 2225, 2226, 2546
PspOMI GGGCCC 2 cut(s) 1608, 2224
PspPPI RGGWCCY 1 cut(s) 810
PspXI VCTCGAGB 1 cut(s) 2087
PstNI CAGNNNCTG 1 cut(s) 1706
PsuI RGATCY 4 cut(s) 355, 718, 1063, 1222
PvuII CAGCTG 1 cut(s) 487
RsaI GTAC 6 cut(s) 494, 1039, 1392, 1704, 2075, 2882
RsaNI GTAC 6 cut(s) 493, 1038, 1391, 1703, 2074, 2881
SacI GAGCTC 1 cut(s) 81
SatI GCNGC 4 cut(s) 42, 275, 1527, 2627
SchI GAGTC 4 cut(s) 1673, 1784, 2099, 2485
ScrFI CCNGG 9 cut(s) 230, 954, 1175, 1211, 1434, 1707, 2003, 2670, 2801
SduI GDGCHC 6 cut(s) 81, 316, 1612, 2035, 2228, 2533
SexAI ACCWGGT 1 cut(s) 2668
SfaNI GCATC 7 cut(s) 53, 55, 146, 2190, 2804, 2826, 2837
SfcI CTRYAG 2 cut(s) 344, 2352
Sfr274I CTCGAG 2 cut(s) 2087, 2330
SfuI TTCGAA 1 cut(s) 1910
SinI GGWCC 5 cut(s) 624, 743, 810, 1161, 1190
SlaI CTCGAG 2 cut(s) 2087, 2330
SmlI CTYRAG 3 cut(s) 2087, 2330, 2342
SmoI CTYRAG 3 cut(s) 2087, 2330, 2342
SsiI CCGC 3 cut(s) 1767, 2037, 2185
SspI AATATT 3 cut(s) 1122, 2428, 2487
SstI GAGCTC 1 cut(s) 81
StyD4I CCNGG 9 cut(s) 228, 952, 1173, 1209, 1432, 1705, 2001, 2668, 2799
StyI CCWWGG 2 cut(s) 141, 2404
TaaI ACNGT 4 cut(s) 836, 1616, 2720, 2854
TaiI ACGT 1 cut(s) 1365
TaqI TCGA 4 cut(s) 505, 1910, 2088, 2331
TatI WGTACW 2 cut(s) 2073, 2880
TfiI GAWTC 9 cut(s) 91, 131, 334, 427, 885, 1324, 1841, 2273, 2746
TscAI CASTG 4 cut(s) 606, 839, 874, 1619
TseFI GTSAC 2 cut(s) 199, 1793
TseI GCWGC 4 cut(s) 41, 274, 1526, 2626
Tsp45I GTSAC 2 cut(s) 199, 1793
TspGWI ACGGA 1 cut(s) 878
TspRI CASTG 4 cut(s) 606, 839, 874, 1619
VpaK11BI GGWCC 5 cut(s) 624, 743, 810, 1161, 1190
XapI RAATTY 7 cut(s) 931, 1201, 1262, 1399, 1438, 2150, 2552
XbaI TCTAGA 3 cut(s) 1219, 1499, 1810
XhoI CTCGAG 2 cut(s) 2087, 2330
XmiI GTMKAC 3 cut(s) 846, 1778, 2569
XmnI GAANNNNTTC 2 cut(s) 1355, 2641
Zsp2I ATGCAT 3 cut(s) 1592, 2605, 2819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.