Rmu_sc0000407.1_g000009

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000407.1
OG0009802
Physical Location & Seq
Reverse (-)
40971 .. 45389
4419 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2292 bp
atggaaactcactcggtttccaagctcttagctttgtacttggtggccgccttgtttgtgggttctgagctgatccaatgcaccactgtgagctatgacaagaaagccattctcatcaatggccggagaagactactcatctccggctcaattcactaccccagaagcacccctgacatgtgggaaggtcttatacagaaagctaaagatggagggttggatgtgattgacacttatgttttctggaatggtcatgaaccttctcctggcaatatgtgggaaggtcttatacagaaagctaaagatggagggttggatgtgattgacacttatgttttctggaatggtcatgaaccttctcctggcaattactattttgaggggagatatgatcttgtacggttcataaggacagtacagaaagcagggctctaccttcatctgcgaattggaccttatgtttgtgcagaatggaattttggaggatttcctgtttggctaaaatatgttcctggaatcagcttcagaacagacaatgggccattcaagatggcaatgcaagggtttacccagaaaattgtccagatgatgaaaaatgaaaggctatttgcatcacaaggtggccccataatcctttctcagattgagaatgaatatgggccagaaggcaatgcacttggagctgctggtcatagctacataaactgggctgccaaaatggccgttggaatgaataccggagtcccatgggtgatgtgcaaggaagatgatgcccccgaccctatgataaatgcatgtaatggtttttactgtgatggtttcactcccaacaaaccttacaagcctactttgtggactgaggcttggagtggctggttttctgagtttggtggcacagttcaccaccgacctgttcaagatttggcatttgcagttgctcgctttatacagaagggtggctcatatgttaattactacatgtatcatggaggtaccaacttcggacgcactgctggaggcccattcattacaacaagctatgactatgatgctccaattgatgaatatggtttgatcagagaacctaaatatactcatctgaaggagcttcatagggctattaagctatctgaacatgctttagtttcttctgatcctactgttacttcattaggaacctatcagcaggcttatgtattcaattcaggaccaagaagctgtgcagcttttctctcgaactttcatatgaaatctgctgcaactgtgacttttaataacatgcactatgatctacctgcttggtccatcagcatccttcctgattgcagaaacgcagtatttaacactgcaaaggtgggagtcccaacttcacgggtgcagatgaccccaacccacaatcgtttgctttattgggagacttatggtgaagatctttcctctctacatgataggtcgagcatatcaactattggacttctagagcagataaatgtcactagagataccagtgactatctgtggtacatgaccaacgttgagatcaattcatgggagtcttttctccatggaggaaaatggccgacacttactctgcagtcagcaggtcatgccgttcatgtctttatcaatggacagttctcaggttcagcctttggaactagggagcagagggaatttacatttacaaaaccagtgaaccttcatgctggaataaacagaattgcactgctcagcatagctgttggactaccgaatgttgggttgcattttgagacatggaaaacaggaatccaaggttcagtttttctggatggtcttagccgaggaaggaaagatttaacaatacagaagtggttatacaaggttggcctaaatggagagacaatgaatttgatctctcccagtggagcctcatccgttgattggatcagagcatcactagctactcataaccagcagttaaaatggtacaaggcctattttaacgcacctttaggaaatgaaccattggctttggacatgcggagcatgggaaagggtcaagtgtggatcaacgggcagagtattggaagatactggatggcttttgctaatggcgactgtagtatgtgcagttacaccgggacataccgggagacaaagtgtcaactaggctgtggtcaaccaacccaacgatggtatcatgtccctcggtcctggttaaagccaacacaaaatttactcgtagtttttgaagaacttggtggtgatccatcgaagattaccctggtcaagagatcactgacaagataa

Protein Analysis

763

Amino Acids

85.58

Weight (kDa)

8.52

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 37 - 91 7.4e-20 Glycosyl hydrolases family 35
Glyco_hydro_35 PF01301 90 - 373 1.5e-109 Glycosyl hydrolases family 35
GHD PF17834 396 - 452 9.6e-23 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 505 - 571 1.7e-08 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 655 - 739 4.6e-27 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45130 AT1G45130
fragaria_vesca FvH4_2g39200 FvH4_2g39200
malus_domestica MD08G1023600.v1.1 MD15G1021100.v1.1
prunus_persica Prupe.1G372800_v2.0.a1
pyrus_communis pycom08g01840 pycom15g01740
rosa_chinensis RchiOBHm_Chr6g0301851
rosa_laevigata RLG00000011216 RLG00000011219
rosa_multiflora Rmu_sc0000407.1_g000009
rosa_roxburghii Rroxscaffold_7G00166340
rosa_rugosa Rorug06G0310300 Rorug06G0310400 Rorug06G0310500
rosa_samantha Rh6BG428900 Rh6CG435500 Rh6DG422300
rosa_wichuraiana Rw6G036570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1303, 1592
Acc65I GGTACC 1 cut(s) 992
AccB1I GGYRCC 1 cut(s) 992
AciI CCGC 2 cut(s) 48, 2023
AclI AACGTT 1 cut(s) 1533
AclWI GGATC 5 cut(s) 67, 1148, 1934, 2057, 2243
AcoI YGGCCR 4 cut(s) 45, 121, 720, 1577
AcsI RAATTY 4 cut(s) 475, 1673, 1888, 2215
AcuI CTGAAG 2 cut(s) 508, 1121
AdeI CACNNNGTG 1 cut(s) 620
AfaI GTAC 6 cut(s) 38, 399, 417, 994, 1523, 1970
AfiI CCNNNNNNNGG 5 cut(s) 266, 362, 1757, 1923, 2175
AflIII ACRYGT 2 cut(s) 177, 978
AgsI TTSAA 4 cut(s) 547, 917, 1201, 2234
AhdI GACNNNNNGTC 1 cut(s) 2142
AjnI CCWGG 5 cut(s) 265, 361, 511, 2195, 2265
AjuI GAANNNNNNNTTGG 2 cut(s) 462, 494
AleI CACNNNNGTG 1 cut(s) 86
Alw26I GTCTC 4 cut(s) 1409, 1766, 1874, 2129
AlwI GGATC 5 cut(s) 67, 1148, 1934, 2057, 2243
ApeKI GCWGC 4 cut(s) 683, 710, 1223, 1256
ApoI RAATTY 4 cut(s) 475, 1673, 1888, 2215
ArsI GACNNNNNNTTYG 4 cut(s) 444, 476, 1873, 1905
Asp718I GGTACC 1 cut(s) 992
AspS9I GGNCC 8 cut(s) 452, 539, 623, 659, 1019, 1208, 1302, 2193
AsuC2I CCSGG 2 cut(s) 2122, 2132
AsuHPI GGTGA 4 cut(s) 763, 893, 1436, 2258
AvaII GGWCC 4 cut(s) 452, 1208, 1302, 2193
BaeI ACNNNNGTAYC 2 cut(s) 2162, 2195
BanI GGYRCC 1 cut(s) 992
BanII GRGCYC 1 cut(s) 432
BarI GAAGNNNNNNTAC 6 cut(s) 177, 209, 273, 305, 1841, 1873
BbsI GAAGAC 1 cut(s) 136
BbvI GCAGC 4 cut(s) 670, 697, 1235, 1243
BccI CCATC 9 cut(s) 203, 299, 544, 809, 1313, 1805, 2074, 2169, 2260
BceAI ACGGC 2 cut(s) 707, 1595
BciT130I CCWGG 5 cut(s) 267, 363, 513, 2197, 2267
BclI TGATCA 1 cut(s) 1074
BcnI CCSGG 2 cut(s) 2122, 2132
BcoDI GTCTC 4 cut(s) 1409, 1766, 1874, 2129
BfaI CTAG 5 cut(s) 1478, 1497, 1659, 1940, 2150
BfmI CTRYAG 2 cut(s) 1592, 2101
BfuAI ACCTGC 2 cut(s) 1303, 1592
BglI GCCNNNNNGGC 1 cut(s) 719
BglII AGATCT 1 cut(s) 1429
BisI GCNGC 5 cut(s) 48, 684, 711, 1224, 1257
BlpI GCTNAGC 1 cut(s) 1730
BlsI GCNGC 5 cut(s) 49, 685, 712, 1225, 1258
Bme1390I CCNGG 7 cut(s) 267, 363, 513, 2122, 2132, 2197, 2267
Bme18I GGWCC 4 cut(s) 452, 1208, 1302, 2193
BmeRI GACNNNNNGTC 1 cut(s) 2142
BmgT120I GGNCC 8 cut(s) 452, 539, 623, 659, 1019, 1208, 1302, 2193
BmiI GGNNCC 4 cut(s) 625, 994, 1177, 1909
BmrFI CCNGG 7 cut(s) 267, 363, 513, 2122, 2132, 2197, 2267
BmrI ACTGGG 2 cut(s) 715, 1896
BmsI GCATC 5 cut(s) 620, 760, 1039, 1320, 1943
BmuI ACTGGG 2 cut(s) 715, 1896
BpiI GAAGAC 1 cut(s) 136
BpmI CTGGAG 1 cut(s) 1035
Bpu1102I GCTNAGC 1 cut(s) 1730
BpuMI CCSGG 2 cut(s) 2122, 2132
BsaJI CCNNGG 6 cut(s) 746, 1564, 1792, 1822, 2189, 2265
BsaWI WCCGGW 1 cut(s) 737
BsaXI ACNNNNNCTCC 2 cut(s) 2126, 2156
Bsc4I CCNNNNNNNGG 5 cut(s) 266, 362, 1757, 1923, 2175
Bse1I ACTGG 5 cut(s) 710, 1506, 1691, 1902, 2081
Bse3DI GCAATG 2 cut(s) 561, 676
BseBI CCWGG 5 cut(s) 267, 363, 513, 2197, 2267
BseDI CCNNGG 6 cut(s) 746, 1564, 1792, 1822, 2189, 2265
BseGI GGATG 6 cut(s) 226, 322, 1311, 1816, 1913, 2085
BseLI CCNNNNNNNGG 5 cut(s) 266, 362, 1757, 1923, 2175
BseMI GCAATG 2 cut(s) 561, 676
BseMII CTCAG 6 cut(s) 57, 653, 849, 873, 1653, 1744
BseNI ACTGG 5 cut(s) 710, 1506, 1691, 1902, 2081
BseXI GCAGC 4 cut(s) 670, 697, 1235, 1243
BsgI GTGCAG 4 cut(s) 486, 1242, 1397, 2131
BshNI GGYRCC 1 cut(s) 992
BsiSI CCGG 5 cut(s) 124, 144, 738, 2121, 2131
BslFI GGGAC 4 cut(s) 728, 1346, 2137, 2171
BslI CCNNNNNNNGG 5 cut(s) 266, 362, 1757, 1923, 2175
BsmAI GTCTC 4 cut(s) 1409, 1766, 1874, 2129
BsmFI GGGAC 4 cut(s) 728, 1346, 2137, 2171
Bsp1286I GDGCHC 1 cut(s) 432
Bsp1720I GCTNAGC 1 cut(s) 1730
Bsp19I CCATGG 2 cut(s) 746, 1564
BspACI CCGC 2 cut(s) 48, 2023
BspCNI CTCAG 6 cut(s) 58, 652, 850, 874, 1652, 1743
BspHI TCATGA 2 cut(s) 253, 349
BspLI GGNNCC 4 cut(s) 625, 994, 1177, 1909
BspMAI CTGCAG 1 cut(s) 1596
BspMI ACCTGC 2 cut(s) 1303, 1592
BspPI GGATC 5 cut(s) 67, 1148, 1934, 2057, 2243
BspT107I GGYRCC 1 cut(s) 992
BsrDI GCAATG 2 cut(s) 561, 676
BsrI ACTGG 5 cut(s) 710, 1506, 1691, 1902, 2081
BssECI CCNNGG 6 cut(s) 746, 1564, 1792, 1822, 2189, 2265
BssT1I CCWWGG 3 cut(s) 746, 1564, 1792
Bst2UI CCWGG 5 cut(s) 267, 363, 513, 2197, 2267
Bst4CI ACNGT 9 cut(s) 88, 402, 415, 812, 898, 1162, 1264, 1635, 2102
BstAPI GCANNNNNTGC 1 cut(s) 1607
BstC8I GCNNGC 2 cut(s) 940, 1188
BstDEI CTNAG 8 cut(s) 28, 66, 639, 858, 882, 1639, 1730, 1817
BstDSI CCRYGG 2 cut(s) 746, 1564
BstF5I GGATG 6 cut(s) 226, 322, 1311, 1816, 1913, 2085
BstMAI GTCTC 4 cut(s) 1409, 1766, 1874, 2129
BstMWI GCNNNNNNNGC 4 cut(s) 680, 719, 1607, 1940
BstNI CCWGG 5 cut(s) 267, 363, 513, 2197, 2267
BstNSI RCATGY 6 cut(s) 181, 798, 982, 1139, 1282, 2023
BstSCI CCNGG 7 cut(s) 265, 361, 511, 2120, 2130, 2195, 2265
BstSFI CTRYAG 2 cut(s) 1592, 2101
BstV1I GCAGC 4 cut(s) 670, 697, 1235, 1243
BstV2I GAAGAC 1 cut(s) 136
BstX2I RGATCY 1 cut(s) 1429
BstYI RGATCY 1 cut(s) 1429
BtgI CCRYGG 2 cut(s) 746, 1564
BtsCI GGATG 6 cut(s) 226, 322, 1311, 1816, 1913, 2085
BtsI GCAGTG 3 cut(s) 1008, 1344, 1724
BtsIMutI CAGTG 8 cut(s) 84, 1008, 1344, 1513, 1698, 1724, 1909, 2279
BveI ACCTGC 2 cut(s) 1303, 1592
Cac8I GCNNGC 2 cut(s) 940, 1188
CciI TCATGA 2 cut(s) 253, 349
Cfr13I GGNCC 8 cut(s) 452, 539, 623, 659, 1019, 1208, 1302, 2193
CseI GACGC 1 cut(s) 1014
Csp6I GTAC 6 cut(s) 37, 398, 416, 993, 1522, 1969
CviQI GTAC 6 cut(s) 37, 398, 416, 993, 1522, 1969
DdeI CTNAG 8 cut(s) 28, 66, 639, 858, 882, 1639, 1730, 1817
DraIII CACNNNGTG 1 cut(s) 620
DriI GACNNNNNGTC 1 cut(s) 2142
EaeI YGGCCR 4 cut(s) 45, 121, 720, 1577
Eam1105I GACNNNNNGTC 1 cut(s) 2142
Eco130I CCWWGG 3 cut(s) 746, 1564, 1792
Eco147I AGGCCT 1 cut(s) 1976
Eco24I GRGCYC 1 cut(s) 432
Eco47I GGWCC 4 cut(s) 452, 1208, 1302, 2193
Eco57I CTGAAG 2 cut(s) 508, 1121
EcoRII CCWGG 5 cut(s) 265, 361, 511, 2195, 2265
EcoT14I CCWWGG 3 cut(s) 746, 1564, 1792
EcoT22I ATGCAT 1 cut(s) 796
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 3 cut(s) 746, 1564, 1792
FaqI GGGAC 4 cut(s) 728, 1346, 2137, 2171
FauNDI CATATG 2 cut(s) 964, 1245
FbaI TGATCA 1 cut(s) 1074
Fnu4HI GCNGC 5 cut(s) 48, 684, 711, 1224, 1257
FokI GGATG 6 cut(s) 233, 329, 1298, 1823, 1900, 2092
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 5 cut(s) 48, 684, 711, 1224, 1257
FspBI CTAG 5 cut(s) 1478, 1497, 1659, 1940, 2150
GluI GCNGC 5 cut(s) 48, 684, 711, 1224, 1257
GsuI CTGGAG 1 cut(s) 1035
HapII CCGG 5 cut(s) 124, 144, 738, 2121, 2131
HgaI GACGC 1 cut(s) 1014
HincII GTYRAC 2 cut(s) 2147, 2162
HindII GTYRAC 2 cut(s) 2147, 2162
HinfI GANTC 5 cut(s) 516, 741, 1359, 1553, 1788
HpaII CCGG 5 cut(s) 124, 144, 738, 2121, 2131
HphI GGTGA 4 cut(s) 763, 893, 1436, 2258
Hpy166II GTNNAC 6 cut(s) 567, 855, 901, 1696, 2147, 2162
Hpy8I GTNNAC 6 cut(s) 567, 855, 901, 1696, 2147, 2162
HpyCH4III ACNGT 9 cut(s) 88, 402, 415, 812, 898, 1162, 1264, 1635, 2102
HpyCH4IV ACGT 1 cut(s) 1533
HpyF10VI GCNNNNNNNGC 4 cut(s) 680, 719, 1607, 1940
HpyF3I CTNAG 8 cut(s) 28, 66, 639, 858, 882, 1639, 1730, 1817
HpySE526I ACGT 1 cut(s) 1533
KpnI GGTACC 1 cut(s) 996
Ksp22I TGATCA 1 cut(s) 1074
LmnI GCTCC 6 cut(s) 680, 1057, 1105, 1663, 1907, 2025
Lsp1109I GCAGC 4 cut(s) 670, 697, 1235, 1243
LweI GCATC 5 cut(s) 620, 760, 1039, 1320, 1943
MaeI CTAG 5 cut(s) 1478, 1497, 1659, 1940, 2150
MaeII ACGT 1 cut(s) 1533
MaeIII GTNAC 5 cut(s) 1162, 1264, 1492, 1508, 2114
MboII GAAGA 7 cut(s) 141, 776, 1140, 1439, 2082, 2246, 2269
MfeI CAATTG 1 cut(s) 1056
MflI RGATCY 1 cut(s) 1429
MhlI GDGCHC 1 cut(s) 432
MlyI GAGTC 3 cut(s) 750, 1368, 1562
MmeI TCCRAC 4 cut(s) 198, 294, 706, 1723
Mph1103I ATGCAT 1 cut(s) 796
MseI TTAA 8 cut(s) 969, 1122, 1272, 1341, 1838, 1961, 1983, 2201
MslI CAYNNNNRTG 1 cut(s) 86
MspI CCGG 5 cut(s) 124, 144, 738, 2121, 2131
MspR9I CCNGG 7 cut(s) 267, 363, 513, 2122, 2132, 2197, 2267
MunI CAATTG 1 cut(s) 1056
MvaI CCWGG 5 cut(s) 267, 363, 513, 2197, 2267
MwoI GCNNNNNNNGC 4 cut(s) 680, 719, 1607, 1940
NciI CCSGG 2 cut(s) 2122, 2132
NcoI CCATGG 2 cut(s) 746, 1564
NdeI CATATG 2 cut(s) 964, 1245
NlaIV GGNNCC 4 cut(s) 625, 994, 1177, 1909
NmeAIII GCCGAG 1 cut(s) 1847
NmuCI GTSAC 3 cut(s) 1264, 1492, 1508
NsiI ATGCAT 1 cut(s) 796
NspI RCATGY 6 cut(s) 181, 798, 982, 1139, 1282, 2023
OliI CACNNNNGTG 1 cut(s) 86
PagI TCATGA 2 cut(s) 253, 349
PceI AGGCCT 1 cut(s) 1976
PciI ACATGT 2 cut(s) 177, 978
PfeI GAWTC 2 cut(s) 516, 1788
PfoI TCCNGGA 1 cut(s) 511
PkrI GCNGC 5 cut(s) 49, 685, 712, 1225, 1258
PleI GAGTC 3 cut(s) 749, 1367, 1561
PpsI GAGTC 3 cut(s) 749, 1367, 1561
PscI ACATGT 2 cut(s) 177, 978
Psp1406I AACGTT 1 cut(s) 1533
Psp6I CCWGG 5 cut(s) 265, 361, 511, 2195, 2265
PspGI CCWGG 5 cut(s) 265, 361, 511, 2195, 2265
PspN4I GGNNCC 4 cut(s) 625, 994, 1177, 1909
PspPI GGNCC 8 cut(s) 452, 539, 623, 659, 1019, 1208, 1302, 2193
PstI CTGCAG 1 cut(s) 1596
PsuI RGATCY 1 cut(s) 1429
RsaI GTAC 6 cut(s) 38, 399, 417, 994, 1523, 1970
RsaNI GTAC 6 cut(s) 37, 398, 416, 993, 1522, 1969
RseI CAYNNNNRTG 1 cut(s) 86
SaqAI TTAA 8 cut(s) 969, 1122, 1272, 1341, 1838, 1961, 1983, 2201
SatI GCNGC 5 cut(s) 48, 684, 711, 1224, 1257
Sau96I GGNCC 8 cut(s) 452, 539, 623, 659, 1019, 1208, 1302, 2193
SchI GAGTC 3 cut(s) 750, 1368, 1562
ScrFI CCNGG 7 cut(s) 267, 363, 513, 2122, 2132, 2197, 2267
SduI GDGCHC 1 cut(s) 432
SfaNI GCATC 5 cut(s) 620, 760, 1039, 1320, 1943
SfcI CTRYAG 2 cut(s) 1592, 2101
SinI GGWCC 4 cut(s) 452, 1208, 1302, 2193
SmiMI CAYNNNNRTG 1 cut(s) 86
SseBI AGGCCT 1 cut(s) 1976
SsiI CCGC 2 cut(s) 48, 2023
SspMI CTAG 5 cut(s) 1478, 1497, 1659, 1940, 2150
StuI AGGCCT 1 cut(s) 1976
StyD4I CCNGG 7 cut(s) 265, 361, 511, 2120, 2130, 2195, 2265
StyI CCWWGG 3 cut(s) 746, 1564, 1792
TaaI ACNGT 9 cut(s) 88, 402, 415, 812, 898, 1162, 1264, 1635, 2102
TaiI ACGT 1 cut(s) 1536
TaqI TCGA 3 cut(s) 1235, 1454, 2255
TaqII GACCGA 1 cut(s) 2181
TatI WGTACW 2 cut(s) 36, 415
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 2 cut(s) 516, 1788
Tru1I TTAA 8 cut(s) 969, 1122, 1272, 1341, 1838, 1961, 1983, 2201
Tru9I TTAA 8 cut(s) 969, 1122, 1272, 1341, 1838, 1961, 1983, 2201
TscAI CASTG 8 cut(s) 91, 1015, 1351, 1513, 1698, 1731, 1909, 2286
TseFI GTSAC 3 cut(s) 1264, 1492, 1508
TseI GCWGC 4 cut(s) 683, 710, 1223, 1256
Tsp45I GTSAC 3 cut(s) 1264, 1492, 1508
TspGWI ACGGA 1 cut(s) 1906
TspRI CASTG 8 cut(s) 91, 1015, 1351, 1513, 1698, 1731, 1909, 2286
VpaK11BI GGWCC 4 cut(s) 452, 1208, 1302, 2193
XapI RAATTY 4 cut(s) 475, 1673, 1888, 2215
XbaI TCTAGA 1 cut(s) 1477
XceI RCATGY 6 cut(s) 181, 798, 982, 1139, 1282, 2023
XcmI CCANNNNNNNNNTGG 1 cut(s) 2172
XspI CTAG 5 cut(s) 1478, 1497, 1659, 1940, 2150
Zsp2I ATGCAT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.