Rmu_sc0000816.1_g000029

Serine threonine-protein kinase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000816.1
OG0007282
Physical Location & Seq
Forward (+)
146703 .. 148034
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1332 bp
atgatttgctggaggttctctgccaatgggactaacatgtccactaagcgaatttataccggtccggccctcaaggatctcagagcaggaaactccagcgtttgcggcctagttgatgggactaatcgcctccaatgctggcagtggcatgggttcaattcaaatacaagtcacagcaacttctcgaggagcatcgcagtgggagacaaatttgtctgcggagtttcagaaatcggagaggtaacttgcactggaagtaataccaaagtgattgatacaaagcatataaggggtaattacagtcatgtaagtgcagggtctaattatgcttgtgcaatctcatggagtaatggtagtttggattgttggggagaaatgaagggtgacaaaccacaagggaagtttacttccctggccttgggcgacaaccgaagctgtggcctgaggcctaatggaacagtcgtttgttggggggaagatggcttcagtttgcctcaaagtttaagagagacttccttcacctcaatagaagcaaaaagcagcgttttctgtggggtcttgtcgaagaatttttccttgtattgctggggaaaccaagtgtttgatacccaagacaatttcttggttttcagtcatgtgaagccagggccatgtagaagagagtgcccattgtatagctccctattaccaggctatggcaacttttgtgaacaaggatatgtagtttgtcaaccttgtgaggggacaactatagtagttcaatcagtgccggggccaacaccgcaaccaccgagggagtctaagtggagcaagaaaatggttgcttttcttgtagtgggttgtgttgggtcctcagctttcttgttggtttgtggctactttgtgttcaagaattgcaaaattagaggttccagaatccatgactcgggtcgattggatgatatcgagagccaagctaatcagcctggttcagggcaactccaaagccaaccagctcaacaaccaccagtgttggagaagcggctaagccacttgattagcctgggagccaatggaggttcattggaggaattttcgttgccagtgctactaaaggccaccaacaacttctcagaagaacacaagattgggacaggcaactatggctcagtctactatgccacattagatgatggccaccgtgaagtggccatcaagcgagccgaatcatcagcgtcctcatcatctgttggcgggcacactaggcgccaggaggacaatgacagtgcatttgtgaatgagcttgagtctctatcacgcctacaccacaagaatctagttcgattgttgggg

Protein Analysis

444

Amino Acids

48.29

Weight (kDa)

8.34

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 370 - 443 1.3e-06 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 37, 212
AccB1I GGYRCC 1 cut(s) 1245
AccB7I CCANNNNNTGG 1 cut(s) 695
AccI GTMKAC 1 cut(s) 1152
AciI CCGC 5 cut(s) 105, 219, 782, 1021, 1233
AclWI GGATC 1 cut(s) 84
AcoI YGGCCR 2 cut(s) 1174, 1188
AcsI RAATTY 4 cut(s) 51, 209, 568, 1070
AcuI CTGAAG 1 cut(s) 469
AcyI GRCGYC 1 cut(s) 1246
AfiI CCNNNNNNNGG 6 cut(s) 418, 695, 740, 925, 971, 1186
AflIII ACRYGT 1 cut(s) 36
AgeI ACCGGT 1 cut(s) 59
AgsI TTSAA 4 cut(s) 157, 162, 761, 889
AjnI CCWGG 6 cut(s) 411, 643, 688, 964, 1041, 1248
AjuI GAANNNNNNNTTGG 2 cut(s) 1110, 1142
AluBI AGCT 6 cut(s) 435, 678, 857, 956, 995, 1282
AluI AGCT 6 cut(s) 435, 678, 857, 956, 995, 1282
Alw26I GTCTC 3 cut(s) 198, 503, 1293
AlwI GGATC 1 cut(s) 84
Ama87I CYCGRG 2 cut(s) 184, 925
ApeKI GCWGC 1 cut(s) 540
ApoI RAATTY 4 cut(s) 51, 209, 568, 1070
ArsI GACNNNNNNTTYG 2 cut(s) 1253, 1285
AsiGI ACCGGT 1 cut(s) 59
AspLEI GCGC 1 cut(s) 1248
AspS9I GGNCC 5 cut(s) 62, 67, 647, 773, 849
AsuC2I CCSGG 1 cut(s) 771
AsuHPI GGTGA 2 cut(s) 395, 511
AvaI CYCGRG 2 cut(s) 184, 925
AvaII GGWCC 2 cut(s) 62, 849
AxyI CCTNAGG 1 cut(s) 443
BaeGI GKGCMC 2 cut(s) 668, 1239
BalI TGGCCA 2 cut(s) 1176, 1190
BanI GGYRCC 1 cut(s) 1245
BbvCI CCTCAGC 1 cut(s) 853
BbvI GCAGC 1 cut(s) 552
BccI CCATC 4 cut(s) 110, 473, 1166, 1199
BciT130I CCWGG 6 cut(s) 413, 645, 690, 966, 1043, 1250
BcnI CCSGG 1 cut(s) 771
BcoDI GTCTC 3 cut(s) 198, 503, 1293
BfaI CTAG 3 cut(s) 110, 1242, 1316
BfmI CTRYAG 1 cut(s) 750
BfoI RGCGCY 1 cut(s) 1249
BisI GCNGC 3 cut(s) 106, 541, 1022
BlpI GCTNAGC 1 cut(s) 1025
BlsI GCNGC 3 cut(s) 107, 542, 1023
Bme1390I CCNGG 7 cut(s) 413, 645, 690, 771, 966, 1043, 1250
Bme18I GGWCC 2 cut(s) 62, 849
BmeT110I CYCGRG 2 cut(s) 184, 925
BmgT120I GGNCC 5 cut(s) 62, 67, 647, 773, 849
BmiI GGNNCC 5 cut(s) 774, 850, 910, 1048, 1247
BmrFI CCNGG 7 cut(s) 413, 645, 690, 771, 966, 1043, 1250
BmsI GCATC 1 cut(s) 201
BoxI GACNNNNGTC 1 cut(s) 927
BpmI CTGGAG 2 cut(s) 31, 79
Bpu10I CCTNAGC 1 cut(s) 853
Bpu1102I GCTNAGC 1 cut(s) 1025
BpuEI CTTGAG 2 cut(s) 56, 1304
BpuMI CCSGG 1 cut(s) 771
BsaHI GRCGYC 1 cut(s) 1246
BsaJI CCNNGG 6 cut(s) 411, 417, 644, 770, 791, 1042
BsaWI WCCGGW 1 cut(s) 59
BsaXI ACNNNNNCTCC 4 cut(s) 337, 367, 788, 818
Bsc4I CCNNNNNNNGG 6 cut(s) 418, 695, 740, 925, 971, 1186
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 3 cut(s) 256, 1007, 1082
Bse21I CCTNAGG 1 cut(s) 443
BseBI CCWGG 6 cut(s) 413, 645, 690, 966, 1043, 1250
BseDI CCNNGG 6 cut(s) 411, 417, 644, 770, 791, 1042
BseGI GGATG 1 cut(s) 943
BseLI CCNNNNNNNGG 6 cut(s) 418, 695, 740, 925, 971, 1186
BseMII CTCAG 5 cut(s) 94, 434, 867, 1125, 1161
BseNI ACTGG 3 cut(s) 256, 1007, 1082
BseRI GAGGAG 1 cut(s) 202
BseSI GKGCMC 2 cut(s) 668, 1239
BseXI GCAGC 1 cut(s) 552
BseYI CCCAGC 1 cut(s) 585
BsgI GTGCAG 1 cut(s) 333
BshNI GGYRCC 1 cut(s) 1245
BshTI ACCGGT 1 cut(s) 59
BsiHKCI CYCGRG 2 cut(s) 184, 925
BsiSI CCGG 3 cut(s) 60, 65, 770
BslFI GGGAC 4 cut(s) 43, 133, 757, 1144
BslI CCNNNNNNNGG 6 cut(s) 418, 695, 740, 925, 971, 1186
BsmAI GTCTC 3 cut(s) 198, 503, 1293
BsmFI GGGAC 4 cut(s) 43, 133, 757, 1144
BsoBI CYCGRG 2 cut(s) 184, 925
Bsp1286I GDGCHC 2 cut(s) 668, 1239
Bsp143I GATC 1 cut(s) 76
Bsp1720I GCTNAGC 1 cut(s) 1025
BspACI CCGC 5 cut(s) 105, 219, 782, 1021, 1233
BspCNI CTCAG 5 cut(s) 93, 435, 866, 1124, 1160
BspLI GGNNCC 5 cut(s) 774, 850, 910, 1048, 1247
BspPI GGATC 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 1245
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 3 cut(s) 256, 1007, 1082
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 6 cut(s) 411, 417, 644, 770, 791, 1042
BssMI GATC 1 cut(s) 76
BssNI GRCGYC 1 cut(s) 1246
BssT1I CCWWGG 1 cut(s) 417
Bst2UI CCWGG 6 cut(s) 413, 645, 690, 966, 1043, 1250
Bst4CI ACNGT 4 cut(s) 302, 460, 1181, 1265
Bst6I CTCTTC 1 cut(s) 652
BstACI GRCGYC 1 cut(s) 1246
BstC8I GCNNGC 3 cut(s) 140, 1200, 1235
BstDEI CTNAG 8 cut(s) 45, 80, 443, 801, 853, 1025, 1111, 1147
BstENI CCTNNNNNAGG 1 cut(s) 969
BstF5I GGATG 1 cut(s) 943
BstH2I RGCGCY 1 cut(s) 1249
BstHHI GCGC 1 cut(s) 1248
BstKTI GATC 1 cut(s) 79
BstMAI GTCTC 3 cut(s) 198, 503, 1293
BstMBI GATC 1 cut(s) 76
BstMWI GCNNNNNNNGC 5 cut(s) 105, 135, 781, 1143, 1243
BstNI CCWGG 6 cut(s) 413, 645, 690, 966, 1043, 1250
BstNSI RCATGY 1 cut(s) 40
BstPAI GACNNNNGTC 1 cut(s) 927
BstSCI CCNGG 7 cut(s) 411, 643, 688, 769, 964, 1041, 1248
BstSFI CTRYAG 1 cut(s) 750
BstSLI GKGCMC 2 cut(s) 668, 1239
BstV1I GCAGC 1 cut(s) 552
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
Bsu36I CCTNAGG 1 cut(s) 443
BtgZI GCGATG 1 cut(s) 178
BtsCI GGATG 1 cut(s) 943
BtsI GCAGTG 2 cut(s) 149, 204
BtsIMutI CAGTG 7 cut(s) 149, 204, 249, 771, 1014, 1089, 1270
Cac8I GCNNGC 3 cut(s) 140, 1200, 1235
CfoI GCGC 1 cut(s) 1248
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 5 cut(s) 62, 67, 647, 773, 849
CpoI CGGWCCG 1 cut(s) 62
CseI GACGC 1 cut(s) 1203
CspAI ACCGGT 1 cut(s) 59
CspCI CAANNNNNGTGG 2 cut(s) 993, 1028
CspI CGGWCCG 1 cut(s) 62
CviAII CATG 7 cut(s) 37, 149, 305, 342, 635, 651, 920
DdeI CTNAG 8 cut(s) 45, 80, 443, 801, 853, 1025, 1111, 1147
DinI GGCGCC 1 cut(s) 1247
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
DrdI GACNNNNNNGTC 2 cut(s) 37, 212
DseDI GACNNNNNNGTC 2 cut(s) 37, 212
EaeI YGGCCR 2 cut(s) 1174, 1188
Eam1104I CTCTTC 1 cut(s) 652
EarI CTCTTC 1 cut(s) 652
Eco130I CCWWGG 1 cut(s) 417
Eco147I AGGCCT 1 cut(s) 448
Eco32I GATATC 1 cut(s) 943
Eco47I GGWCC 2 cut(s) 62, 849
Eco57I CTGAAG 1 cut(s) 469
Eco81I CCTNAGG 1 cut(s) 443
Eco88I CYCGRG 2 cut(s) 184, 925
EcoNI CCTNNNNNAGG 1 cut(s) 969
EcoO109I RGGNCCY 1 cut(s) 849
EcoRII CCWGG 6 cut(s) 411, 643, 688, 964, 1041, 1248
EcoRV GATATC 1 cut(s) 943
EcoT14I CCWWGG 1 cut(s) 417
EgeI GGCGCC 1 cut(s) 1247
EheI GGCGCC 1 cut(s) 1247
ErhI CCWWGG 1 cut(s) 417
FaeI CATG 7 cut(s) 40, 152, 308, 345, 638, 654, 923
FalI AAGNNNNNCTT 2 cut(s) 496, 528
FaqI GGGAC 4 cut(s) 43, 133, 757, 1144
FatI CATG 7 cut(s) 36, 148, 304, 341, 634, 650, 919
FauI CCCGC 1 cut(s) 1226
FblI GTMKAC 1 cut(s) 1152
Fnu4HI GCNGC 3 cut(s) 106, 541, 1022
FokI GGATG 1 cut(s) 950
Fsp4HI GCNGC 3 cut(s) 106, 541, 1022
FspBI CTAG 3 cut(s) 110, 1242, 1316
GlaI GCGC 1 cut(s) 1247
GluI GCNGC 3 cut(s) 106, 541, 1022
GsaI CCCAGC 1 cut(s) 589
GsuI CTGGAG 2 cut(s) 31, 79
HaeII RGCGCY 1 cut(s) 1249
HapII CCGG 3 cut(s) 60, 65, 770
HgaI GACGC 1 cut(s) 1203
HhaI GCGC 1 cut(s) 1248
Hin1I GRCGYC 1 cut(s) 1246
Hin1II CATG 7 cut(s) 40, 152, 308, 345, 638, 654, 923
Hin6I GCGC 1 cut(s) 1246
HinP1I GCGC 1 cut(s) 1246
HincII GTYRAC 1 cut(s) 731
HindII GTYRAC 1 cut(s) 731
HinfI GANTC 6 cut(s) 797, 915, 923, 1205, 1286, 1312
HpaII CCGG 3 cut(s) 60, 65, 770
HphI GGTGA 2 cut(s) 395, 511
Hpy166II GTNNAC 5 cut(s) 42, 405, 710, 731, 1153
Hpy188I TCNGA 4 cut(s) 83, 229, 236, 1114
Hpy188III TCNNGA 4 cut(s) 184, 889, 912, 946
Hpy8I GTNNAC 5 cut(s) 42, 405, 710, 731, 1153
HpyAV CCTTC 2 cut(s) 373, 526
HpyCH4III ACNGT 4 cut(s) 302, 460, 1181, 1265
HpyCH4V TGCA 5 cut(s) 249, 314, 335, 897, 1268
HpyF10VI GCNNNNNNNGC 5 cut(s) 105, 135, 781, 1143, 1243
HpyF3I CTNAG 8 cut(s) 45, 80, 443, 801, 853, 1025, 1111, 1147
Hsp92I GRCGYC 1 cut(s) 1246
Hsp92II CATG 7 cut(s) 40, 152, 308, 345, 638, 654, 923
HspAI GCGC 1 cut(s) 1246
KasI GGCGCC 1 cut(s) 1245
Kzo9I GATC 1 cut(s) 76
LmnI GCTCC 4 cut(s) 189, 683, 807, 1046
Lsp1109I GCAGC 1 cut(s) 552
LweI GCATC 1 cut(s) 201
MaeI CTAG 3 cut(s) 110, 1242, 1316
MaeIII GTNAC 3 cut(s) 170, 241, 383
MalI GATC 1 cut(s) 78
MboI GATC 1 cut(s) 76
MboII GAAGA 4 cut(s) 488, 577, 669, 1127
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 2 cut(s) 668, 1239
MlsI TGGCCA 2 cut(s) 1176, 1190
MluNI TGGCCA 2 cut(s) 1176, 1190
Mly113I GGCGCC 1 cut(s) 1246
MlyI GAGTC 3 cut(s) 806, 917, 1295
MmeI TCCRAC 1 cut(s) 993
Mox20I TGGCCA 2 cut(s) 1176, 1190
MscI TGGCCA 2 cut(s) 1176, 1190
MseI TTAA 1 cut(s) 503
MslI CAYNNNNRTG 2 cut(s) 197, 309
Msp20I TGGCCA 2 cut(s) 1176, 1190
MspI CCGG 3 cut(s) 60, 65, 770
MspR9I CCNGG 7 cut(s) 413, 645, 690, 771, 966, 1043, 1250
MvaI CCWGG 6 cut(s) 413, 645, 690, 966, 1043, 1250
MwoI GCNNNNNNNGC 5 cut(s) 105, 135, 781, 1143, 1243
NarI GGCGCC 1 cut(s) 1246
NciI CCSGG 1 cut(s) 771
NdeII GATC 1 cut(s) 76
NlaIII CATG 7 cut(s) 40, 152, 308, 345, 638, 654, 923
NlaIV GGNNCC 5 cut(s) 774, 850, 910, 1048, 1247
NmuCI GTSAC 2 cut(s) 170, 383
NspI RCATGY 1 cut(s) 40
PaeR7I CTCGAG 1 cut(s) 184
PceI AGGCCT 1 cut(s) 448
PciI ACATGT 1 cut(s) 36
PfeI GAWTC 3 cut(s) 915, 1205, 1312
PflMI CCANNNNNTGG 1 cut(s) 695
PinAI ACCGGT 1 cut(s) 59
PkrI GCNGC 3 cut(s) 107, 542, 1023
PleI GAGTC 3 cut(s) 805, 917, 1294
PluTI GGCGCC 1 cut(s) 1249
PpsI GAGTC 3 cut(s) 805, 917, 1294
PpuMI RGGWCCY 1 cut(s) 849
PscI ACATGT 1 cut(s) 36
PshAI GACNNNNGTC 1 cut(s) 927
Psp5II RGGWCCY 1 cut(s) 849
Psp6I CCWGG 6 cut(s) 411, 643, 688, 964, 1041, 1248
PspFI CCCAGC 1 cut(s) 585
PspGI CCWGG 6 cut(s) 411, 643, 688, 964, 1041, 1248
PspN4I GGNNCC 5 cut(s) 774, 850, 910, 1048, 1247
PspPI GGNCC 5 cut(s) 62, 67, 647, 773, 849
PspPPI RGGWCCY 1 cut(s) 849
PsuI RGATCY 1 cut(s) 76
RseI CAYNNNNRTG 2 cut(s) 197, 309
Rsr2I CGGWCCG 1 cut(s) 62
RsrII CGGWCCG 1 cut(s) 62
SaqAI TTAA 1 cut(s) 503
SatI GCNGC 3 cut(s) 106, 541, 1022
Sau3AI GATC 1 cut(s) 76
Sau96I GGNCC 5 cut(s) 62, 67, 647, 773, 849
SchI GAGTC 3 cut(s) 806, 917, 1295
ScrFI CCNGG 7 cut(s) 413, 645, 690, 771, 966, 1043, 1250
SduI GDGCHC 2 cut(s) 668, 1239
SfaNI GCATC 1 cut(s) 201
SfcI CTRYAG 1 cut(s) 750
SfoI GGCGCC 1 cut(s) 1247
Sfr274I CTCGAG 1 cut(s) 184
SinI GGWCC 2 cut(s) 62, 849
SlaI CTCGAG 1 cut(s) 184
SmiMI CAYNNNNRTG 2 cut(s) 197, 309
SmlI CTYRAG 3 cut(s) 71, 184, 1283
SmoI CTYRAG 3 cut(s) 71, 184, 1283
SseBI AGGCCT 1 cut(s) 448
SsiI CCGC 5 cut(s) 105, 219, 782, 1021, 1233
SspDI GGCGCC 1 cut(s) 1245
SspMI CTAG 3 cut(s) 110, 1242, 1316
StuI AGGCCT 1 cut(s) 448
StyD4I CCNGG 7 cut(s) 411, 643, 688, 769, 964, 1041, 1248
StyI CCWWGG 1 cut(s) 417
TaaI ACNGT 4 cut(s) 302, 460, 1181, 1265
TaqI TCGA 5 cut(s) 185, 563, 931, 945, 1321
TauI GCSGC 2 cut(s) 108, 1024
TfiI GAWTC 3 cut(s) 915, 1205, 1312
Tru1I TTAA 1 cut(s) 503
Tru9I TTAA 1 cut(s) 503
TscAI CASTG 7 cut(s) 149, 204, 256, 771, 1014, 1089, 1270
TseFI GTSAC 2 cut(s) 170, 383
TseI GCWGC 1 cut(s) 540
Tsp45I GTSAC 2 cut(s) 170, 383
TspDTI ATGAA 2 cut(s) 392, 1050
TspRI CASTG 7 cut(s) 149, 204, 256, 771, 1014, 1089, 1270
Van91I CCANNNNNTGG 1 cut(s) 695
VpaK11BI GGWCC 2 cut(s) 62, 849
XagI CCTNNNNNAGG 1 cut(s) 969
XapI RAATTY 4 cut(s) 51, 209, 568, 1070
XceI RCATGY 1 cut(s) 40
XhoI CTCGAG 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 1152
XspI CTAG 3 cut(s) 110, 1242, 1316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.