Rmu_sc0000970.1_g000001

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000970.1
OG0017773
Physical Location & Seq
Reverse (-)
1 .. 734
734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000970.1_g000001.1.cds

Sequence Viewer

Length: 693 bp
atgtacaaggctcgatattttccggagcattcattttggtcagctgtgccgcatagttcaccctcttattcatggcggagcatcttctctacaagagatttacttagtcaaggatcttattggcaaattggggatggacgcaatgtctctgtatggtgcaacccttggataccaggtttagcttcctttaaacctacttcaggtacttcggtggtggatacagatttgaaggttagtcattttatatcgtctccaggtgtttggaatgcaaatgaggtacataataattttagtactggagaggccgaggcaattttggccatacctttaagtgtgcgtgctttagatgatagattggcatggaggctagaagggaatggtaaattttcagtaaagactgcttaccgttttgcctatagtcactctctctcaaggattcctattcttcttcctattgtttcgaacttttggaagaagctatggaacacactagttccaaattctactaaagtacacatttggaaaatttgtcacaacatattaccatcattagaccgtattgcttctaaaggagttgaactggagtctatggtgtgtgtcctctgtgataatgagatggagactacggtgcatctatgcagagactgtttatgcactaagcaagtgttgcagcctaatgcatcttttacccag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

231

Amino Acids

26.12

Weight (kDa)

8.68

Isoelectric Point (pI)

32.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 22
AciI CCGC 2 cut(s) 50, 76
AclWI GGATC 1 cut(s) 121
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 3 cut(s) 383, 499, 525
AcuI CTGAAG 1 cut(s) 183
AfaI GTAC 5 cut(s) 5, 205, 279, 295, 513
AfiI CCNNNNNNNGG 1 cut(s) 200
AgsI TTSAA 2 cut(s) 229, 578
AhdI GACNNNNNGTC 1 cut(s) 143
AhlI ACTAGT 1 cut(s) 490
AjnI CCWGG 2 cut(s) 172, 253
AluBI AGCT 3 cut(s) 44, 182, 478
AluI AGCT 3 cut(s) 44, 182, 478
Alw26I GTCTC 4 cut(s) 151, 255, 614, 636
AlwI GGATC 1 cut(s) 121
AlwNI CAGNNNCTG 1 cut(s) 645
Aor13HI TCCGGA 1 cut(s) 22
AoxI GGCC 2 cut(s) 303, 318
ApeKI GCWGC 1 cut(s) 670
ApoI RAATTY 3 cut(s) 383, 499, 525
AsuHPI GGTGA 1 cut(s) 51
AsuII TTCGAA 1 cut(s) 461
BaeI ACNNNNGTAYC 2 cut(s) 195, 228
BalI TGGCCA 1 cut(s) 320
BbvI GCAGC 1 cut(s) 682
BccI CCATC 3 cut(s) 128, 553, 610
BciT130I CCWGG 2 cut(s) 174, 255
BciVI GTATCC 2 cut(s) 162, 211
BcoDI GTCTC 4 cut(s) 151, 255, 614, 636
BcuI ACTAGT 1 cut(s) 490
BfaI CTAG 2 cut(s) 368, 491
BfmI CTRYAG 1 cut(s) 415
BfuI GTATCC 2 cut(s) 162, 211
BisI GCNGC 2 cut(s) 50, 671
BlsI GCNGC 2 cut(s) 51, 672
BmcAI AGTACT 1 cut(s) 295
Bme1390I CCNGG 2 cut(s) 174, 255
BmeRI GACNNNNNGTC 1 cut(s) 143
BmrFI CCNGG 2 cut(s) 174, 255
BmsI GCATC 3 cut(s) 90, 640, 689
BpmI CTGGAG 3 cut(s) 237, 318, 602
Bpu14I TTCGAA 1 cut(s) 461
BpuEI CTTGAG 1 cut(s) 415
BsaJI CCNNGG 2 cut(s) 164, 306
BsaWI WCCGGW 1 cut(s) 22
Bsc4I CCNNNNNNNGG 1 cut(s) 200
Bse1I ACTGG 2 cut(s) 301, 585
Bse3DI GCAATG 1 cut(s) 148
BseAI TCCGGA 1 cut(s) 22
BseBI CCWGG 2 cut(s) 174, 255
BseDI CCNNGG 2 cut(s) 164, 306
BseGI GGATG 1 cut(s) 139
BseLI CCNNNNNNNGG 1 cut(s) 200
BseMI GCAATG 1 cut(s) 148
BseNI ACTGG 2 cut(s) 301, 585
BseXI GCAGC 1 cut(s) 682
BshFI GGCC 2 cut(s) 305, 320
BsiSI CCGG 1 cut(s) 23
BslI CCNNNNNNNGG 1 cut(s) 200
BsmAI GTCTC 4 cut(s) 151, 255, 614, 636
BsmBI CGTCTC 1 cut(s) 255
BsmI GAATGC 2 cut(s) 28, 271
BsnI GGCC 2 cut(s) 305, 320
Bsp119I TTCGAA 1 cut(s) 461
Bsp13I TCCGGA 1 cut(s) 22
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 113
BspACI CCGC 2 cut(s) 50, 76
BspANI GGCC 2 cut(s) 305, 320
BspEI TCCGGA 1 cut(s) 22
BspPI GGATC 1 cut(s) 121
BspT104I TTCGAA 1 cut(s) 461
BsrDI GCAATG 1 cut(s) 148
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 301, 585
BssECI CCNNGG 2 cut(s) 164, 306
BssMI GATC 1 cut(s) 113
BssT1I CCWWGG 1 cut(s) 164
Bst2UI CCWGG 2 cut(s) 174, 255
Bst4CI ACNGT 4 cut(s) 407, 557, 628, 647
BstAPI GCANNNNNTGC 1 cut(s) 667
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 461
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 2 cut(s) 104, 657
BstENI CCTNNNNNAGG 1 cut(s) 198
BstF5I GGATG 1 cut(s) 139
BstKTI GATC 1 cut(s) 116
BstMAI GTCTC 4 cut(s) 151, 255, 614, 636
BstMBI GATC 1 cut(s) 113
BstMWI GCNNNNNNNGC 2 cut(s) 317, 667
BstNI CCWGG 2 cut(s) 174, 255
BstSCI CCNGG 2 cut(s) 172, 253
BstSFI CTRYAG 1 cut(s) 415
BstV1I GCAGC 1 cut(s) 682
BstX2I RGATCY 1 cut(s) 113
BstXI CCANNNNNNTGG 1 cut(s) 261
BstYI RGATCY 1 cut(s) 113
BsuI GTATCC 2 cut(s) 162, 211
BsuRI GGCC 2 cut(s) 305, 320
BtsCI GGATG 1 cut(s) 139
Cac8I GCNNGC 1 cut(s) 339
CaiI CAGNNNCTG 1 cut(s) 645
CseI GACGC 1 cut(s) 147
CsiI ACCWGGT 1 cut(s) 172
Csp6I GTAC 5 cut(s) 4, 204, 278, 294, 512
CviAII CATG 2 cut(s) 72, 360
CviJI RGCY 8 cut(s) 11, 44, 182, 305, 320, 367, 478, 673
CviKI_1 RGCY 8 cut(s) 11, 44, 182, 305, 320, 367, 478, 673
CviQI GTAC 5 cut(s) 4, 204, 278, 294, 512
DdeI CTNAG 2 cut(s) 104, 657
DpnI GATC 1 cut(s) 115
DpnII GATC 1 cut(s) 113
DraI TTTAAA 1 cut(s) 190
DriI GACNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 318
Eam1105I GACNNNNNGTC 1 cut(s) 143
EciI GGCGGA 1 cut(s) 91
Eco130I CCWWGG 1 cut(s) 164
Eco57I CTGAAG 1 cut(s) 183
EcoNI CCTNNNNNAGG 1 cut(s) 198
EcoRII CCWGG 2 cut(s) 172, 253
EcoT14I CCWWGG 1 cut(s) 164
EcoT22I ATGCAT 1 cut(s) 682
ErhI CCWWGG 1 cut(s) 164
Esp3I CGTCTC 1 cut(s) 255
FaeI CATG 2 cut(s) 75, 363
FatI CATG 2 cut(s) 71, 359
Fnu4HI GCNGC 2 cut(s) 50, 671
FokI GGATG 1 cut(s) 146
Fsp4HI GCNGC 2 cut(s) 50, 671
FspBI CTAG 2 cut(s) 368, 491
GluI GCNGC 2 cut(s) 50, 671
GsuI CTGGAG 3 cut(s) 237, 318, 602
HaeIII GGCC 2 cut(s) 305, 320
HapII CCGG 1 cut(s) 23
HgaI GACGC 1 cut(s) 147
Hin1II CATG 2 cut(s) 75, 363
HinfI GANTC 2 cut(s) 436, 584
HpaII CCGG 1 cut(s) 23
HphI GGTGA 1 cut(s) 51
Hpy166II GTNNAC 2 cut(s) 59, 514
Hpy188III TCNNGA 1 cut(s) 23
Hpy8I GTNNAC 2 cut(s) 59, 514
HpyAV CCTTC 2 cut(s) 223, 365
HpyCH4III ACNGT 4 cut(s) 407, 557, 628, 647
HpyCH4V TGCA 7 cut(s) 159, 269, 631, 639, 654, 670, 680
HpyF10VI GCNNNNNNNGC 2 cut(s) 317, 667
HpyF3I CTNAG 2 cut(s) 104, 657
Hsp92II CATG 2 cut(s) 75, 363
Kpn2I TCCGGA 1 cut(s) 22
Kzo9I GATC 1 cut(s) 113
LmnI GCTCC 2 cut(s) 25, 78
LpnPI CCDG 8 cut(s) 36, 159, 186, 186, 240, 267, 282, 566
Lsp1109I GCAGC 1 cut(s) 682
LweI GCATC 3 cut(s) 90, 640, 689
MabI ACCWGGT 1 cut(s) 172
MaeI CTAG 2 cut(s) 368, 491
MaeIII GTNAC 2 cut(s) 419, 530
MalI GATC 1 cut(s) 115
MboI GATC 1 cut(s) 113
MboII GAAGA 4 cut(s) 76, 437, 440, 484
MflI RGATCY 1 cut(s) 113
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 6 cut(s) 126, 286, 312, 383, 499, 525
MluNI TGGCCA 1 cut(s) 320
MlyI GAGTC 1 cut(s) 593
MnlI CCTC 6 cut(s) 73, 268, 295, 301, 357, 611
Mox20I TGGCCA 1 cut(s) 320
Mph1103I ATGCAT 1 cut(s) 682
MroI TCCGGA 1 cut(s) 22
MscI TGGCCA 1 cut(s) 320
MseI TTAA 2 cut(s) 189, 329
Msp20I TGGCCA 1 cut(s) 320
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 1 cut(s) 23
MspR9I CCNGG 2 cut(s) 174, 255
Mva1269I GAATGC 2 cut(s) 28, 271
MvaI CCWGG 2 cut(s) 174, 255
MwoI GCNNNNNNNGC 2 cut(s) 317, 667
NdeII GATC 1 cut(s) 113
NlaIII CATG 2 cut(s) 75, 363
NmeAIII GCCGAG 1 cut(s) 331
NmuCI GTSAC 2 cut(s) 419, 530
NsiI ATGCAT 1 cut(s) 682
NspV TTCGAA 1 cut(s) 461
PctI GAATGC 2 cut(s) 28, 271
PfeI GAWTC 1 cut(s) 436
PkrI GCNGC 2 cut(s) 51, 672
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
Psp6I CCWGG 2 cut(s) 172, 253
PspGI CCWGG 2 cut(s) 172, 253
PstNI CAGNNNCTG 1 cut(s) 645
PsuI RGATCY 1 cut(s) 113
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 5 cut(s) 5, 205, 279, 295, 513
RsaNI GTAC 5 cut(s) 4, 204, 278, 294, 512
SaqAI TTAA 2 cut(s) 189, 329
SatI GCNGC 2 cut(s) 50, 671
Sau3AI GATC 1 cut(s) 113
ScaI AGTACT 1 cut(s) 295
SchI GAGTC 1 cut(s) 593
ScrFI CCNGG 2 cut(s) 174, 255
SexAI ACCWGGT 1 cut(s) 172
SfaNI GCATC 3 cut(s) 90, 640, 689
SfcI CTRYAG 1 cut(s) 415
SfuI TTCGAA 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 430
SmoI CTYRAG 1 cut(s) 430
SpeI ACTAGT 1 cut(s) 490
Sse9I AATT 6 cut(s) 126, 286, 312, 383, 499, 525
SsiI CCGC 2 cut(s) 50, 76
SspMI CTAG 2 cut(s) 368, 491
StyD4I CCNGG 2 cut(s) 172, 253
StyI CCWWGG 1 cut(s) 164
TaaI ACNGT 4 cut(s) 407, 557, 628, 647
TaqI TCGA 2 cut(s) 13, 461
TasI AATT 6 cut(s) 126, 286, 312, 383, 499, 525
TatI WGTACW 3 cut(s) 3, 293, 511
TauI GCSGC 1 cut(s) 52
TfiI GAWTC 1 cut(s) 436
Tru1I TTAA 2 cut(s) 189, 329
Tru9I TTAA 2 cut(s) 189, 329
TseFI GTSAC 2 cut(s) 419, 530
TseI GCWGC 1 cut(s) 670
Tsp45I GTSAC 2 cut(s) 419, 530
TspDTI ATGAA 2 cut(s) 21, 60
XagI CCTNNNNNAGG 1 cut(s) 198
XapI RAATTY 3 cut(s) 383, 499, 525
XspI CTAG 2 cut(s) 368, 491
ZrmI AGTACT 1 cut(s) 295
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.