Rmu_sc0009966.1_g000007

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009966.1
OG0017773
Physical Location & Seq
Forward (+)
24825 .. 25921
1097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009966.1_g000007.1.cds

Sequence Viewer

Length: 864 bp
atgctagcaaagcaagcctggtggttgattaacaatccttactctttggttgctcgaattttcaaggccaagcactatccagaagattctttttggactgtggctcctcacccttcttcttctttttcatggtggagtattttttctacaagggatttgttaagaagtgggacttattggcagattgattttaggcctaagcagggtaatgctgcgtcgaatgagcttactttggtgagtgacttaattgatcagacaggtcagtggaatactgctttgatccgtgataattttcctgtggaagaggcagaggcaatattggccattcccttatccttcagagaggttgatgatagatttgcttggcatctcgagaagaatggaaagtttacggtgaaaactgcgtatagattttcattctctgcttcttcttctcgtagtccctttgaactaacggttggtgcgactttctggaaaaagatgtggaaggtaacaattctaaattcagctaaaattcatgtttggagagtatgtcatgatatattgccttccatggaacggttggcttctcggagagtagcactggagaccttgatatgtcctctttattccaatgctatagagactaatctacatttatgtcgtgattgtccctttactaaacaactgctccagtccaatgatgtattgtaccagggtactttcacaataaatgagtttcgttttcataacttgaaaactgaaccaagtggagtaagaacacacatggttgtaaggtggaagtcaccgccggtggggtggtttaaagtgaatgttgatggggcttacaattatgcttcaagaagaggtagtggtgggttgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

287

Amino Acids

33.13

Weight (kDa)

9.44

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 788
AclWI GGATC 1 cut(s) 274
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 3 cut(s) 57, 502, 513
AcuI CTGAAG 1 cut(s) 322
AfaI GTAC 2 cut(s) 692, 700
AfiI CCNNNNNNNGG 3 cut(s) 203, 558, 794
AgsI TTSAA 4 cut(s) 64, 449, 736, 840
AjnI CCWGG 2 cut(s) 17, 693
AjuI GAANNNNNNNTTGG 2 cut(s) 441, 473
AluBI AGCT 2 cut(s) 226, 509
AluI AGCT 2 cut(s) 226, 509
Alw26I GTCTC 2 cut(s) 581, 617
AlwI GGATC 1 cut(s) 274
Ama87I CYCGRG 1 cut(s) 371
AoxI GGCC 3 cut(s) 66, 194, 321
ApeKI GCWGC 1 cut(s) 212
ApoI RAATTY 3 cut(s) 57, 502, 513
AsuHPI GGTGA 4 cut(s) 101, 247, 406, 777
AsuNHI GCTAGC 1 cut(s) 4
AvaI CYCGRG 1 cut(s) 371
BalI TGGCCA 1 cut(s) 323
BbvI GCAGC 1 cut(s) 199
BccI CCATC 1 cut(s) 812
BciT130I CCWGG 2 cut(s) 19, 695
BclI TGATCA 1 cut(s) 250
BcoDI GTCTC 2 cut(s) 581, 617
BfaI CTAG 1 cut(s) 5
BfmI CTRYAG 1 cut(s) 618
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 2 cut(s) 19, 695
BmeT110I CYCGRG 1 cut(s) 371
BmiI GGNNCC 1 cut(s) 105
BmrFI CCNGG 2 cut(s) 19, 695
BmsI GCATC 1 cut(s) 376
BmtI GCTAGC 1 cut(s) 8
BpmI CTGGAG 2 cut(s) 605, 656
Bpu10I CCTNAGC 1 cut(s) 198
BsaI GGTCTC 1 cut(s) 581
BsaJI CCNNGG 2 cut(s) 552, 694
BsaXI ACNNNNNCTCC 6 cut(s) 88, 118, 517, 547, 654, 684
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 558, 794
Bse118I RCCGGY 1 cut(s) 790
Bse1I ACTGG 2 cut(s) 588, 673
BseBI CCWGG 2 cut(s) 19, 695
BseDI CCNNGG 2 cut(s) 552, 694
BseLI CCNNNNNNNGG 3 cut(s) 203, 558, 794
BseNI ACTGG 2 cut(s) 588, 673
BseRI GAGGAG 1 cut(s) 96
BseXI GCAGC 1 cut(s) 199
BshFI GGCC 3 cut(s) 68, 196, 323
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 1 cut(s) 791
BslFI GGGAC 3 cut(s) 184, 426, 636
BslI CCNNNNNNNGG 3 cut(s) 203, 558, 794
BsmAI GTCTC 2 cut(s) 581, 617
BsmFI GGGAC 3 cut(s) 184, 426, 636
BsnI GGCC 3 cut(s) 68, 196, 323
Bso31I GGTCTC 1 cut(s) 581
BsoBI CYCGRG 1 cut(s) 371
Bsp143I GATC 2 cut(s) 250, 279
Bsp19I CCATGG 1 cut(s) 552
BspACI CCGC 1 cut(s) 788
BspANI GGCC 3 cut(s) 68, 196, 323
BspHI TCATGA 1 cut(s) 535
BspLI GGNNCC 1 cut(s) 105
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 1 cut(s) 274
BspTNI GGTCTC 1 cut(s) 581
BsrFI RCCGGY 1 cut(s) 790
BsrI ACTGG 2 cut(s) 588, 673
BssAI RCCGGY 1 cut(s) 790
BssECI CCNNGG 2 cut(s) 552, 694
BssMI GATC 2 cut(s) 250, 279
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 2 cut(s) 19, 695
Bst4CI ACNGT 4 cut(s) 100, 394, 457, 561
Bst6I CTCTTC 2 cut(s) 297, 838
BstC8I GCNNGC 2 cut(s) 6, 15
BstDEI CTNAG 1 cut(s) 198
BstDSI CCRYGG 1 cut(s) 552
BstKTI GATC 2 cut(s) 253, 282
BstMAI GTCTC 2 cut(s) 581, 617
BstMBI GATC 2 cut(s) 250, 279
BstMWI GCNNNNNNNGC 3 cut(s) 10, 14, 320
BstNI CCWGG 2 cut(s) 19, 695
BstSCI CCNGG 2 cut(s) 17, 693
BstSFI CTRYAG 1 cut(s) 618
BstV1I GCAGC 1 cut(s) 199
BsuRI GGCC 3 cut(s) 68, 196, 323
BtgI CCRYGG 1 cut(s) 552
BtsIMutI CAGTG 2 cut(s) 269, 581
Cac8I GCNNGC 2 cut(s) 6, 15
CciI TCATGA 1 cut(s) 535
Cfr10I RCCGGY 1 cut(s) 790
CseI GACGC 1 cut(s) 204
Csp6I GTAC 2 cut(s) 691, 699
CspCI CAANNNNNGTGG 1 cut(s) 37
CviAII CATG 5 cut(s) 129, 518, 536, 553, 766
CviJI RGCY 9 cut(s) 17, 68, 104, 196, 226, 323, 509, 566, 824
CviKI_1 RGCY 9 cut(s) 17, 68, 104, 196, 226, 323, 509, 566, 824
CviQI GTAC 2 cut(s) 691, 699
DdeI CTNAG 1 cut(s) 198
DpnI GATC 2 cut(s) 252, 281
DpnII GATC 2 cut(s) 250, 279
DraI TTTAAA 1 cut(s) 805
EaeI YGGCCR 1 cut(s) 321
Eam1104I CTCTTC 2 cut(s) 297, 838
EarI CTCTTC 2 cut(s) 297, 838
Eco130I CCWWGG 1 cut(s) 552
Eco147I AGGCCT 1 cut(s) 196
Eco31I GGTCTC 1 cut(s) 581
Eco57I CTGAAG 1 cut(s) 322
Eco88I CYCGRG 1 cut(s) 371
EcoRII CCWGG 2 cut(s) 17, 693
EcoT14I CCWWGG 1 cut(s) 552
ErhI CCWWGG 1 cut(s) 552
FaeI CATG 5 cut(s) 132, 521, 539, 556, 769
FalI AAGNNNNNCTT 2 cut(s) 157, 189
FaqI GGGAC 3 cut(s) 184, 426, 636
FatI CATG 5 cut(s) 128, 517, 535, 552, 765
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 1 cut(s) 213
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 1 cut(s) 5
GluI GCNGC 1 cut(s) 213
GsuI CTGGAG 2 cut(s) 605, 656
HaeIII GGCC 3 cut(s) 68, 196, 323
HapII CCGG 1 cut(s) 791
HgaI GACGC 1 cut(s) 204
Hin1II CATG 5 cut(s) 132, 521, 539, 556, 769
HinfI GANTC 1 cut(s) 86
HpaII CCGG 1 cut(s) 791
HphI GGTGA 4 cut(s) 101, 247, 406, 777
Hpy166II GTNNAC 1 cut(s) 390
Hpy188I TCNGA 3 cut(s) 255, 341, 573
Hpy188III TCNNGA 7 cut(s) 80, 371, 373, 472, 536, 644, 840
Hpy8I GTNNAC 1 cut(s) 390
Hpy99I CGWCG 1 cut(s) 220
HpyAV CCTTC 4 cut(s) 123, 346, 481, 558
HpyCH4III ACNGT 4 cut(s) 100, 394, 457, 561
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 14, 320
HpyF3I CTNAG 1 cut(s) 198
Hsp92II CATG 5 cut(s) 132, 521, 539, 556, 769
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 2 cut(s) 250, 279
LmnI GCTCC 2 cut(s) 109, 675
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 1 cut(s) 376
MaeI CTAG 1 cut(s) 5
MaeIII GTNAC 3 cut(s) 239, 490, 783
MalI GATC 2 cut(s) 252, 281
MboI GATC 2 cut(s) 250, 279
MboII GAAGA 8 cut(s) 95, 108, 111, 314, 388, 420, 423, 855
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 7 cut(s) 57, 246, 289, 495, 502, 513, 829
MluNI TGGCCA 1 cut(s) 323
MnlI CCTC 6 cut(s) 117, 298, 304, 337, 612, 839
Mox20I TGGCCA 1 cut(s) 323
MscI TGGCCA 1 cut(s) 323
MseI TTAA 4 cut(s) 30, 161, 245, 804
Msp20I TGGCCA 1 cut(s) 323
MspI CCGG 1 cut(s) 791
MspR9I CCNGG 2 cut(s) 19, 695
MvaI CCWGG 2 cut(s) 19, 695
MwoI GCNNNNNNNGC 3 cut(s) 10, 14, 320
NcoI CCATGG 1 cut(s) 552
NdeII GATC 2 cut(s) 250, 279
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 5 cut(s) 132, 521, 539, 556, 769
NlaIV GGNNCC 1 cut(s) 105
NmuCI GTSAC 2 cut(s) 239, 783
PaeR7I CTCGAG 1 cut(s) 371
PagI TCATGA 1 cut(s) 535
PceI AGGCCT 1 cut(s) 196
PcsI WCGNNNNNNNCGW 1 cut(s) 461
PfeI GAWTC 1 cut(s) 86
PkrI GCNGC 1 cut(s) 214
Psp6I CCWGG 2 cut(s) 17, 693
PspGI CCWGG 2 cut(s) 17, 693
PspN4I GGNNCC 1 cut(s) 105
RsaI GTAC 2 cut(s) 692, 700
RsaNI GTAC 2 cut(s) 691, 699
SaqAI TTAA 4 cut(s) 30, 161, 245, 804
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 2 cut(s) 250, 279
ScrFI CCNGG 2 cut(s) 19, 695
SetI ASST 8 cut(s) 228, 262, 348, 492, 511, 593, 779, 850
SfaNI GCATC 1 cut(s) 376
SfcI CTRYAG 1 cut(s) 618
Sfr274I CTCGAG 1 cut(s) 371
SgrAI CRCCGGYG 1 cut(s) 790
SlaI CTCGAG 1 cut(s) 371
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 7 cut(s) 57, 246, 289, 495, 502, 513, 829
SseBI AGGCCT 1 cut(s) 196
SsiI CCGC 1 cut(s) 788
SspI AATATT 1 cut(s) 318
SspMI CTAG 1 cut(s) 5
StuI AGGCCT 1 cut(s) 196
StyD4I CCNGG 2 cut(s) 17, 693
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 4 cut(s) 100, 394, 457, 561
TaqI TCGA 3 cut(s) 55, 218, 372
TasI AATT 7 cut(s) 57, 246, 289, 495, 502, 513, 829
TfiI GAWTC 1 cut(s) 86
Tru1I TTAA 4 cut(s) 30, 161, 245, 804
Tru9I TTAA 4 cut(s) 30, 161, 245, 804
TscAI CASTG 2 cut(s) 269, 588
TseFI GTSAC 2 cut(s) 239, 783
TseI GCWGC 1 cut(s) 212
Tsp45I GTSAC 2 cut(s) 239, 783
TspDTI ATGAA 4 cut(s) 117, 405, 506, 716
TspGWI ACGGA 1 cut(s) 272
TspRI CASTG 2 cut(s) 269, 588
XapI RAATTY 3 cut(s) 57, 502, 513
XcmI CCANNNNNNNNNTGG 1 cut(s) 559
XhoI CTCGAG 1 cut(s) 371
XspI CTAG 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.