Rmu_sc0011109.1_g000011

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0011109.1
OG0017773
Physical Location & Seq
Reverse (-)
30476 .. 31262
787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0011109.1_g000011.1.cds

Sequence Viewer

Length: 603 bp
atgtgtgctcggtttttgtggggaagctctatgaataaccagaagattcattggaaaccttggaaggctcttagtaaccctaaggaggaagggggtttggggtttcgtagtttgactgattttaataatgccatgctagctaagcaggcctggagggtggttaacaaccctctatctttgagtgctcggatcttcaaggctccttattttccagaagattctttttggactgtggaacctcatgcttctccgtcttactcatggacaagcatcttctcaactagagatatgctacgcaaggtagagggcactaatgacattttagctatcccattatctacaagggtggtagaagaccgagtggcttggcatcttgagaatagtggtaagttcactgtaaagactgcctataggttttcattttctacttctacttctacttctcgtagcccctttgctttgatggtgggtacaagcttctggaagaagatttggaaagttaacattccgagctcagccaagatccatgtgtggcaagtttgtcacgatatattgccatgtagggagcgtcctgtgtccaatatgcccactttgttctgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

200

Amino Acids

22.95

Weight (kDa)

9.83

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 197, 517
AfaI GTAC 1 cut(s) 472
AfiI CCNNNNNNNGG 1 cut(s) 85
AgsI TTSAA 1 cut(s) 196
AjnI CCWGG 1 cut(s) 149
AluBI AGCT 5 cut(s) 27, 140, 326, 477, 513
AluI AGCT 5 cut(s) 27, 140, 326, 477, 513
Alw21I GWGCWC 3 cut(s) 10, 187, 515
AlwI GGATC 2 cut(s) 197, 517
AoxI GGCC 1 cut(s) 147
AsuNHI GCTAGC 1 cut(s) 136
AxyI CCTNAGG 1 cut(s) 81
BaeGI GKGCMC 1 cut(s) 311
BanII GRGCYC 1 cut(s) 515
BbsI GAAGAC 1 cut(s) 360
Bbv12I GWGCWC 3 cut(s) 10, 187, 515
BccI CCATC 1 cut(s) 457
BciT130I CCWGG 1 cut(s) 151
BfaI CTAG 2 cut(s) 137, 282
BfmI CTRYAG 1 cut(s) 409
BlpI GCTNAGC 2 cut(s) 141, 514
Bme1390I CCNGG 1 cut(s) 151
BmiI GGNNCC 2 cut(s) 201, 237
BmrFI CCNGG 1 cut(s) 151
BmsI GCATC 2 cut(s) 279, 379
BmtI GCTAGC 1 cut(s) 140
BpiI GAAGAC 1 cut(s) 360
BpmI CTGGAG 1 cut(s) 172
Bpu1102I GCTNAGC 2 cut(s) 141, 514
BpuEI CTTGAG 1 cut(s) 395
BsaJI CCNNGG 1 cut(s) 59
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse21I CCTNAGG 1 cut(s) 81
BseBI CCWGG 1 cut(s) 151
BseDI CCNNGG 1 cut(s) 59
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMII CTCAG 1 cut(s) 528
BseSI GKGCMC 1 cut(s) 311
BshFI GGCC 1 cut(s) 149
BsiHKAI GWGCWC 3 cut(s) 10, 187, 515
BslI CCNNNNNNNGG 1 cut(s) 85
BsnI GGCC 1 cut(s) 149
Bsp1286I GDGCHC 4 cut(s) 10, 187, 311, 515
Bsp143I GATC 2 cut(s) 189, 522
Bsp1720I GCTNAGC 2 cut(s) 141, 514
BspANI GGCC 1 cut(s) 149
BspCNI CTCAG 1 cut(s) 527
BspLI GGNNCC 2 cut(s) 201, 237
BspOI GCTAGC 1 cut(s) 140
BspPI GGATC 2 cut(s) 197, 517
BssECI CCNNGG 1 cut(s) 59
BssMI GATC 2 cut(s) 189, 522
BssT1I CCWWGG 1 cut(s) 59
Bst2UI CCWGG 1 cut(s) 151
Bst4CI ACNGT 2 cut(s) 232, 397
BstC8I GCNNGC 2 cut(s) 138, 147
BstDEI CTNAG 4 cut(s) 71, 81, 141, 514
BstKTI GATC 2 cut(s) 192, 525
BstMBI GATC 2 cut(s) 189, 522
BstMWI GCNNNNNNNGC 3 cut(s) 137, 142, 146
BstNI CCWGG 1 cut(s) 151
BstSCI CCNGG 1 cut(s) 149
BstSFI CTRYAG 1 cut(s) 409
BstSLI GKGCMC 1 cut(s) 311
BstV2I GAAGAC 1 cut(s) 360
BstX2I RGATCY 2 cut(s) 189, 522
BstYI RGATCY 2 cut(s) 189, 522
Bsu36I CCTNAGG 1 cut(s) 81
BsuRI GGCC 1 cut(s) 149
BtsIMutI CAGTG 1 cut(s) 393
Cac8I GCNNGC 2 cut(s) 138, 147
CseI GACGC 1 cut(s) 557
Csp6I GTAC 1 cut(s) 471
CviAII CATG 5 cut(s) 133, 242, 261, 527, 558
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 4 cut(s) 71, 81, 141, 514
DpnI GATC 2 cut(s) 191, 524
DpnII GATC 2 cut(s) 189, 522
Ecl136II GAGCTC 1 cut(s) 513
Eco130I CCWWGG 1 cut(s) 59
Eco147I AGGCCT 1 cut(s) 149
Eco24I GRGCYC 1 cut(s) 515
Eco53kI GAGCTC 1 cut(s) 513
Eco81I CCTNAGG 1 cut(s) 81
EcoICRI GAGCTC 1 cut(s) 513
EcoRII CCWGG 1 cut(s) 149
EcoT14I CCWWGG 1 cut(s) 59
EcoT38I GRGCYC 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 59
FaeI CATG 5 cut(s) 136, 245, 264, 530, 561
FatI CATG 5 cut(s) 132, 241, 260, 526, 557
FriOI GRGCYC 1 cut(s) 515
FspBI CTAG 2 cut(s) 137, 282
GsuI CTGGAG 1 cut(s) 172
HaeIII GGCC 1 cut(s) 149
HgaI GACGC 1 cut(s) 557
Hin1II CATG 5 cut(s) 136, 245, 264, 530, 561
HincII GTYRAC 2 cut(s) 163, 502
HindII GTYRAC 2 cut(s) 163, 502
HindIII AAGCTT 1 cut(s) 475
HinfI GANTC 2 cut(s) 46, 218
HpaI GTTAAC 2 cut(s) 163, 502
Hpy166II GTNNAC 3 cut(s) 163, 393, 502
Hpy188I TCNGA 2 cut(s) 189, 510
Hpy188III TCNNGA 4 cut(s) 212, 374, 481, 545
Hpy8I GTNNAC 3 cut(s) 163, 393, 502
HpyAV CCTTC 2 cut(s) 58, 83
HpyCH4III ACNGT 2 cut(s) 232, 397
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 142, 146
HpyF3I CTNAG 4 cut(s) 71, 81, 141, 514
Hsp92II CATG 5 cut(s) 136, 245, 264, 530, 561
KspAI GTTAAC 2 cut(s) 163, 502
Kzo9I GATC 2 cut(s) 189, 522
LmnI GCTCC 2 cut(s) 205, 565
LpnPI CCDG 7 cut(s) 53, 131, 136, 163, 225, 466, 585
LweI GCATC 2 cut(s) 279, 379
MaeI CTAG 2 cut(s) 137, 282
MaeIII GTNAC 2 cut(s) 74, 542
MalI GATC 2 cut(s) 191, 524
MboI GATC 2 cut(s) 189, 522
MboII GAAGA 7 cut(s) 55, 184, 227, 265, 365, 496, 499
MflI RGATCY 2 cut(s) 189, 522
MhlI GDGCHC 4 cut(s) 10, 187, 311, 515
MnlI CCTC 5 cut(s) 79, 147, 180, 249, 298
MseI TTAA 3 cut(s) 123, 162, 501
MspR9I CCNGG 1 cut(s) 151
MvaI CCWGG 1 cut(s) 151
MwoI GCNNNNNNNGC 3 cut(s) 137, 142, 146
NdeII GATC 2 cut(s) 189, 522
NheI GCTAGC 1 cut(s) 136
NlaIII CATG 5 cut(s) 136, 245, 264, 530, 561
NlaIV GGNNCC 2 cut(s) 201, 237
NmuCI GTSAC 1 cut(s) 542
PceI AGGCCT 1 cut(s) 149
PfeI GAWTC 2 cut(s) 46, 218
Psp124BI GAGCTC 1 cut(s) 515
Psp6I CCWGG 1 cut(s) 149
PspGI CCWGG 1 cut(s) 149
PspN4I GGNNCC 2 cut(s) 201, 237
PsuI RGATCY 2 cut(s) 189, 522
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
SacI GAGCTC 1 cut(s) 515
SaqAI TTAA 3 cut(s) 123, 162, 501
Sau3AI GATC 2 cut(s) 189, 522
ScrFI CCNGG 1 cut(s) 151
SduI GDGCHC 4 cut(s) 10, 187, 311, 515
SetI ASST 9 cut(s) 29, 61, 142, 241, 303, 328, 416, 479, 515
SfaNI GCATC 2 cut(s) 279, 379
SfcI CTRYAG 1 cut(s) 409
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SseBI AGGCCT 1 cut(s) 149
SspMI CTAG 2 cut(s) 137, 282
SstI GAGCTC 1 cut(s) 515
StuI AGGCCT 1 cut(s) 149
StyD4I CCNGG 1 cut(s) 149
StyI CCWWGG 1 cut(s) 59
TaaI ACNGT 2 cut(s) 232, 397
TaqII GACCGA 1 cut(s) 372
TfiI GAWTC 2 cut(s) 46, 218
Tru1I TTAA 3 cut(s) 123, 162, 501
Tru9I TTAA 3 cut(s) 123, 162, 501
TscAI CASTG 1 cut(s) 400
TseFI GTSAC 1 cut(s) 542
Tsp45I GTSAC 1 cut(s) 542
TspDTI ATGAA 3 cut(s) 38, 47, 408
TspGWI ACGGA 1 cut(s) 240
TspRI CASTG 1 cut(s) 400
XspI CTAG 2 cut(s) 137, 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.