Rmu_sc0001035.1_g000077

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001035.1
OG0017647
Physical Location & Seq
Reverse (-)
379718 .. 380272
555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001035.1_g000077.1.cds

Sequence Viewer

Length: 555 bp
atgagtcctcgagccccaactattcatcatttgctatttgccaatgatagctttatattcggagaagcctctgtggtggaatactctacattcaagtctattttgaatgtgtatgagcatgcttctggccaaaggattaatttagagaagagcagtgttgtcttcagccagaatgtgcacccggatactaaagctaatttagcttctattttgggggttcagtgtgtggaggaccatggcagatatctagggcttccacttcatgtgggaaaatcaaaagtggtaattttttcttatcttaaggaaagattaactaagaagctgattatttggcgcacaaaaatcctgagttctgcagggaaggagattctgattaaggcagtggcacaagttattccaacatatgttatgagttgttatatgcttcctaaaggtctttgtgaggatctccaccaactttgtgctcaatttttttggggaggttcagattctaatccaaaattcactggcggtcatgggataggatgtgcctgtctaaaaggtagggaggtttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

184

Amino Acids

20.34

Weight (kDa)

9.03

Isoelectric Point (pI)

28.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 510
AclWI GGATC 1 cut(s) 453
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 1 cut(s) 500
AcuI CTGAAG 1 cut(s) 148
AflII CTTAAG 1 cut(s) 299
AgsI TTSAA 2 cut(s) 94, 106
AluBI AGCT 4 cut(s) 51, 194, 203, 322
AluI AGCT 4 cut(s) 51, 194, 203, 322
Alw21I GWGCWC 2 cut(s) 180, 466
Alw44I GTGCAC 1 cut(s) 176
AlwI GGATC 1 cut(s) 453
Ama87I CYCGRG 1 cut(s) 9
AoxI GGCC 1 cut(s) 127
ApaLI GTGCAC 1 cut(s) 176
ApoI RAATTY 1 cut(s) 500
AseI ATTAAT 1 cut(s) 138
AspLEI GCGC 1 cut(s) 336
AspS9I GGNCC 1 cut(s) 232
AsuC2I CCSGG 1 cut(s) 182
AvaI CYCGRG 1 cut(s) 9
AvaII GGWCC 1 cut(s) 232
BaeGI GKGCMC 1 cut(s) 180
BalI TGGCCA 1 cut(s) 129
BanII GRGCYC 1 cut(s) 16
BbsI GAAGAC 1 cut(s) 154
Bbv12I GWGCWC 2 cut(s) 180, 466
BciVI GTATCC 1 cut(s) 178
BcnI CCSGG 1 cut(s) 182
BfaI CTAG 1 cut(s) 248
BfmI CTRYAG 1 cut(s) 354
BfrI CTTAAG 1 cut(s) 299
BfuI GTATCC 1 cut(s) 178
Bme1390I CCNGG 1 cut(s) 182
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 9
BmgT120I GGNCC 1 cut(s) 232
BmrFI CCNGG 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 154
BpuMI CCSGG 1 cut(s) 182
BsaBI GATNNNNATC 1 cut(s) 492
BsaJI CCNNGG 1 cut(s) 235
Bse1I ACTGG 1 cut(s) 511
Bse8I GATNNNNATC 1 cut(s) 492
BseDI CCNNGG 1 cut(s) 235
BseGI GGATG 1 cut(s) 530
BseJI GATNNNNATC 1 cut(s) 492
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 1 cut(s) 511
BseSI GKGCMC 1 cut(s) 180
BshFI GGCC 1 cut(s) 129
BsiHKAI GWGCWC 2 cut(s) 180, 466
BsiHKCI CYCGRG 1 cut(s) 9
BsiSI CCGG 1 cut(s) 182
BsnI GGCC 1 cut(s) 129
BsoBI CYCGRG 1 cut(s) 9
Bsp1286I GDGCHC 3 cut(s) 16, 180, 466
Bsp143I GATC 1 cut(s) 445
Bsp19I CCATGG 1 cut(s) 235
BspACI CCGC 1 cut(s) 510
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 1 cut(s) 339
BspMAI CTGCAG 1 cut(s) 358
BspPI GGATC 1 cut(s) 453
BspQI GCTCTTC 1 cut(s) 143
BspTI CTTAAG 1 cut(s) 299
BsrI ACTGG 1 cut(s) 511
BssECI CCNNGG 1 cut(s) 235
BssMI GATC 1 cut(s) 445
BssT1I CCWWGG 1 cut(s) 235
Bst6I CTCTTC 1 cut(s) 143
BstAFI CTTAAG 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 2 cut(s) 315, 347
BstDSI CCRYGG 1 cut(s) 235
BstF5I GGATG 1 cut(s) 530
BstHHI GCGC 1 cut(s) 336
BstKTI GATC 1 cut(s) 448
BstMBI GATC 1 cut(s) 445
BstMWI GCNNNNNNNGC 1 cut(s) 200
BstNSI RCATGY 1 cut(s) 122
BstSCI CCNGG 1 cut(s) 180
BstSFI CTRYAG 1 cut(s) 354
BstSLI GKGCMC 1 cut(s) 180
BstV2I GAAGAC 1 cut(s) 154
BstX2I RGATCY 1 cut(s) 445
BstYI RGATCY 1 cut(s) 445
BsuI GTATCC 1 cut(s) 178
BsuRI GGCC 1 cut(s) 129
BtgI CCRYGG 1 cut(s) 235
BtsCI GGATG 1 cut(s) 530
BtsI GCAGTG 2 cut(s) 160, 387
BtsIMutI CAGTG 4 cut(s) 160, 227, 387, 504
Cac8I GCNNGC 1 cut(s) 120
CfoI GCGC 1 cut(s) 336
Cfr13I GGNCC 1 cut(s) 232
CspCI CAANNNNNGTGG 2 cut(s) 440, 475
CviAII CATG 4 cut(s) 119, 236, 263, 515
CviJI RGCY 9 cut(s) 14, 51, 68, 129, 168, 194, 203, 253, 322
CviKI_1 RGCY 9 cut(s) 14, 51, 68, 129, 168, 194, 203, 253, 322
DdeI CTNAG 2 cut(s) 315, 347
DpnI GATC 1 cut(s) 447
DpnII GATC 1 cut(s) 445
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 16
Eco32I GATATC 1 cut(s) 245
Eco47I GGWCC 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 148
Eco88I CYCGRG 1 cut(s) 9
EcoRV GATATC 1 cut(s) 245
EcoT14I CCWWGG 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 235
FaeI CATG 4 cut(s) 122, 239, 266, 518
FatI CATG 4 cut(s) 118, 235, 262, 514
FauNDI CATATG 1 cut(s) 403
FokI GGATG 1 cut(s) 537
FriOI GRGCYC 1 cut(s) 16
FspBI CTAG 1 cut(s) 248
GlaI GCGC 1 cut(s) 335
HaeIII GGCC 1 cut(s) 129
HapII CCGG 1 cut(s) 182
HhaI GCGC 1 cut(s) 336
Hin1II CATG 4 cut(s) 122, 239, 266, 518
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HinfI GANTC 3 cut(s) 4, 367, 488
HpaII CCGG 1 cut(s) 182
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 3 cut(s) 62, 372, 487
Hpy188III TCNNGA 1 cut(s) 346
Hpy8I GTNNAC 1 cut(s) 178
HpyAV CCTTC 1 cut(s) 355
HpyCH4V TGCA 2 cut(s) 178, 356
HpyF10VI GCNNNNNNNGC 1 cut(s) 200
HpyF3I CTNAG 2 cut(s) 315, 347
Hsp92II CATG 4 cut(s) 122, 239, 266, 518
HspAI GCGC 1 cut(s) 334
Kzo9I GATC 1 cut(s) 445
LguI GCTCTTC 1 cut(s) 143
LpnPI CCDG 7 cut(s) 111, 182, 195, 342, 359, 492, 544
MaeI CTAG 1 cut(s) 248
MalI GATC 1 cut(s) 447
MboI GATC 1 cut(s) 445
MboII GAAGA 2 cut(s) 154, 160
MflI RGATCY 1 cut(s) 445
MhlI GDGCHC 3 cut(s) 16, 180, 466
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 5 cut(s) 139, 196, 285, 467, 500
MluNI TGGCCA 1 cut(s) 129
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 422
MnlI CCTC 6 cut(s) 18, 79, 223, 436, 473, 541
Mox20I TGGCCA 1 cut(s) 129
MscI TGGCCA 1 cut(s) 129
MseI TTAA 4 cut(s) 138, 300, 311, 375
Msp20I TGGCCA 1 cut(s) 129
MspCI CTTAAG 1 cut(s) 299
MspI CCGG 1 cut(s) 182
MspR9I CCNGG 1 cut(s) 182
MwoI GCNNNNNNNGC 1 cut(s) 200
NciI CCSGG 1 cut(s) 182
NcoI CCATGG 1 cut(s) 235
NdeI CATATG 1 cut(s) 403
NdeII GATC 1 cut(s) 445
NlaIII CATG 4 cut(s) 122, 239, 266, 518
NspI RCATGY 1 cut(s) 122
PaeI GCATGC 1 cut(s) 122
PaeR7I CTCGAG 1 cut(s) 9
PciSI GCTCTTC 1 cut(s) 143
PfeI GAWTC 2 cut(s) 367, 488
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PshBI ATTAAT 1 cut(s) 138
PspPI GGNCC 1 cut(s) 232
PspXI VCTCGAGB 1 cut(s) 9
PstI CTGCAG 1 cut(s) 358
PsuI RGATCY 1 cut(s) 445
SapI GCTCTTC 1 cut(s) 143
SaqAI TTAA 4 cut(s) 138, 300, 311, 375
Sau3AI GATC 1 cut(s) 445
Sau96I GGNCC 1 cut(s) 232
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 1 cut(s) 182
SduI GDGCHC 3 cut(s) 16, 180, 466
SetI ASST 8 cut(s) 53, 196, 205, 324, 436, 484, 544, 552
SfcI CTRYAG 1 cut(s) 354
Sfr274I CTCGAG 1 cut(s) 9
SinI GGWCC 1 cut(s) 232
SlaI CTCGAG 1 cut(s) 9
SmlI CTYRAG 2 cut(s) 9, 299
SmoI CTYRAG 2 cut(s) 9, 299
SphI GCATGC 1 cut(s) 122
Sse9I AATT 5 cut(s) 139, 196, 285, 467, 500
SsiI CCGC 1 cut(s) 510
SspMI CTAG 1 cut(s) 248
StyD4I CCNGG 1 cut(s) 180
StyI CCWWGG 1 cut(s) 235
TaqI TCGA 1 cut(s) 10
TasI AATT 5 cut(s) 139, 196, 285, 467, 500
TfiI GAWTC 2 cut(s) 367, 488
Tru1I TTAA 4 cut(s) 138, 300, 311, 375
Tru9I TTAA 4 cut(s) 138, 300, 311, 375
TscAI CASTG 4 cut(s) 160, 227, 387, 511
TspDTI ATGAA 2 cut(s) 14, 251
TspRI CASTG 4 cut(s) 160, 227, 387, 511
Vha464I CTTAAG 1 cut(s) 299
VneI GTGCAC 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 232
VspI ATTAAT 1 cut(s) 138
XapI RAATTY 1 cut(s) 500
XceI RCATGY 1 cut(s) 122
XhoI CTCGAG 1 cut(s) 9
XspI CTAG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.