Rorug06G0073500

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0017647
Physical Location & Seq
Reverse (-)
9784618 .. 9787029
2412 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0073500.1

Sequence Viewer

Length: 1053 bp
ATGGATGCATACATGGAAGTTGATGCTGAAGATGATGGCAATGCCATAGAGATGGGTCTCTCATATGAGGAAACTCAACAAACTACCATCAACGATGTGCACAATGATATGGAGTATTTTGTGACTGAAGCTATACATGACACAGATTCTGAAGATGAGGATGGTAATACAGTGAACAGACCACGAGGAAAGAACAAGCCAAATTGGGGAAGAAGAGGTTCTCTTGAGATCATGAAATTTAACAATCATGGTCAAGCAGTGGAACCAGAACTAACAGTTGCAAGGTGGCAACGTCATTTGGGTCAAATGGTGAAAGACCACACTCTTTTTGGTCTAGATGTTTTGGACTGGCGCGAGTTTAGGAAAAGCAAAGTTCTAGATGAGGCTTGGTCCAGGATAAAGAGAACCATTGATTGGTCTGATCCGGATACTAGAAACAAAATCAGGAAGATACGATATACAACGGAGAGAAAATTGAATGATCGCTGGAAGCACTACAAAGCAACACTTCGACAGGTTTGGTATGCTCCAAACTGGAGAACTGAGGAGCGTTTCCATTGTGGTGATAAAAGGGTAAACAATACTCAATGGGCCAAACTCGTTAACTATTGGGAGACGCCAAAGGGACAGCGCCGAACAATTGCAAACAAGGGCAATCGTGCAAAGAAACGAATGAACCACACAACAGGAACAAGAACGTTTGCTCAAGTGAGGAACCAATATAAGAGAGAACATGAAGGTCTGGAACCTAATAGATGCACCTTGTTTGAGCTAACCCACACACGATCCAATGGGAAGCCTGTGGATAAAGAGTCAGAGAAAGCAATGATGGATATGAGGCAGAAGATCAGTGGCATCAAAGATGCTAAAAGAGCAAATCAGCTTCCTGAAACCATTAGTAACATTGAGATAAGTGAAGTGTATGCTTCTGTGCTTGGAAAGGAGAAATATGGAGTAGTTAGAGGATTTGGGTTAGGAGTCCGACTTCATGATGTCCCTGTGATGTGTTGGTGGAAAACGGAAGTGCTCAATTATTGCAGGTTCAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

350

Amino Acids

41.38

Weight (kDa)

9.27

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 194 - 327 1.1e-27 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1029
AccB7I CCANNNNNTGG 1 cut(s) 414
AccII CGCG 1 cut(s) 354
AccIII TCCGGA 1 cut(s) 424
AclI AACGTT 1 cut(s) 698
AclWI GGATC 2 cut(s) 416, 780
AcsI RAATTY 1 cut(s) 236
AcuI CTGAAG 3 cut(s) 48, 147, 171
AcyI GRCGYC 1 cut(s) 617
AfiI CCNNNNNNNGG 2 cut(s) 206, 414
AgsI TTSAA 2 cut(s) 478, 1045
AjnI CCWGG 1 cut(s) 392
AjuI GAANNNNNNNTTGG 2 cut(s) 397, 429
AluBI AGCT 3 cut(s) 131, 772, 883
AluI AGCT 3 cut(s) 131, 772, 883
Alw21I GWGCWC 2 cut(s) 102, 1029
Alw26I GTCTC 2 cut(s) 62, 608
Alw44I GTGCAC 1 cut(s) 98
AlwI GGATC 2 cut(s) 416, 780
AlwNI CAGNNNCTG 1 cut(s) 149
Aor13HI TCCGGA 1 cut(s) 424
AoxI GGCC 1 cut(s) 591
ApaLI GTGCAC 1 cut(s) 98
ApoI RAATTY 1 cut(s) 236
Asp700I GAANNNNTTC 1 cut(s) 217
AspLEI GCGC 2 cut(s) 354, 633
AspS9I GGNCC 2 cut(s) 390, 591
AsuHPI GGTGA 2 cut(s) 322, 575
AvaII GGWCC 1 cut(s) 390
BaeGI GKGCMC 1 cut(s) 102
BauI CACGAG 1 cut(s) 183
Bbv12I GWGCWC 2 cut(s) 102, 1029
BccI CCATC 5 cut(s) 29, 46, 95, 155, 823
BciT130I CCWGG 1 cut(s) 394
BciVI GTATCC 1 cut(s) 421
BcoDI GTCTC 2 cut(s) 62, 608
BfaI CTAG 3 cut(s) 335, 377, 432
BfoI RGCGCY 1 cut(s) 634
BfuAI ACCTGC 1 cut(s) 1029
BfuI GTATCC 1 cut(s) 421
Bme1390I CCNGG 1 cut(s) 394
Bme18I GGWCC 1 cut(s) 390
BmgT120I GGNCC 2 cut(s) 390, 591
BmiI GGNNCC 3 cut(s) 264, 716, 747
BmrFI CCNGG 1 cut(s) 394
BmsI GCATC 4 cut(s) 13, 746, 853, 864
BpmI CTGGAG 1 cut(s) 556
BpuEI CTTGAG 2 cut(s) 245, 690
BsaHI GRCGYC 1 cut(s) 617
BsaI GGTCTC 1 cut(s) 62
BsaWI WCCGGW 1 cut(s) 424
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 414
Bse1I ACTGG 2 cut(s) 353, 539
Bse3DI GCAATG 2 cut(s) 46, 831
BseAI TCCGGA 1 cut(s) 424
BseBI CCWGG 1 cut(s) 394
BseGI GGATG 2 cut(s) 10, 166
BseLI CCNNNNNNNGG 2 cut(s) 206, 414
BseMI GCAATG 2 cut(s) 46, 831
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 2 cut(s) 353, 539
BseRI GAGGAG 1 cut(s) 560
BseSI GKGCMC 1 cut(s) 102
Bsh1236I CGCG 1 cut(s) 354
BshFI GGCC 1 cut(s) 593
BsiHKAI GWGCWC 2 cut(s) 102, 1029
BsiSI CCGG 1 cut(s) 425
BslFI GGGAC 2 cut(s) 639, 980
BslI CCNNNNNNNGG 2 cut(s) 206, 414
BsmAI GTCTC 2 cut(s) 62, 608
BsmBI CGTCTC 1 cut(s) 608
BsmFI GGGAC 2 cut(s) 639, 980
BsnI GGCC 1 cut(s) 593
Bso31I GGTCTC 1 cut(s) 62
Bsp1286I GDGCHC 2 cut(s) 102, 1029
Bsp13I TCCGGA 1 cut(s) 424
Bsp143I GATC 5 cut(s) 228, 421, 481, 785, 846
BspANI GGCC 1 cut(s) 593
BspCNI CTCAG 1 cut(s) 535
BspEI TCCGGA 1 cut(s) 424
BspFNI CGCG 1 cut(s) 354
BspHI TCATGA 2 cut(s) 231, 988
BspLI GGNNCC 3 cut(s) 264, 716, 747
BspMI ACCTGC 1 cut(s) 1029
BspPI GGATC 2 cut(s) 416, 780
BspTNI GGTCTC 1 cut(s) 62
BsrDI GCAATG 2 cut(s) 46, 831
BsrI ACTGG 2 cut(s) 353, 539
BssMI GATC 5 cut(s) 228, 421, 481, 785, 846
BssNI GRCGYC 1 cut(s) 617
BssSI CACGAG 1 cut(s) 183
Bst2BI CACGAG 1 cut(s) 183
Bst2UI CCWGG 1 cut(s) 394
Bst4CI ACNGT 2 cut(s) 172, 277
Bst6I CTCTTC 1 cut(s) 208
BstACI GRCGYC 1 cut(s) 617
BstDEI CTNAG 1 cut(s) 543
BstF5I GGATG 2 cut(s) 10, 166
BstFNI CGCG 1 cut(s) 354
BstH2I RGCGCY 1 cut(s) 634
BstHHI GCGC 2 cut(s) 354, 633
BstKTI GATC 5 cut(s) 231, 424, 484, 788, 849
BstMAI GTCTC 2 cut(s) 62, 608
BstMBI GATC 5 cut(s) 228, 421, 481, 785, 846
BstMWI GCNNNNNNNGC 1 cut(s) 872
BstNI CCWGG 1 cut(s) 394
BstSCI CCNGG 1 cut(s) 392
BstSLI GKGCMC 1 cut(s) 102
BstUI CGCG 1 cut(s) 354
BstXI CCANNNNNNTGG 1 cut(s) 52
BsuI GTATCC 1 cut(s) 421
BsuRI GGCC 1 cut(s) 593
BtsCI GGATG 2 cut(s) 10, 166
BtsI GCAGTG 1 cut(s) 264
BtsIMutI CAGTG 4 cut(s) 177, 264, 856, 1048
BveI ACCTGC 1 cut(s) 1029
CaiI CAGNNNCTG 1 cut(s) 149
CciI TCATGA 2 cut(s) 231, 988
CfoI GCGC 2 cut(s) 354, 633
Cfr13I GGNCC 2 cut(s) 390, 591
CseI GACGC 1 cut(s) 625
CviAII CATG 6 cut(s) 13, 137, 232, 248, 734, 989
CviJI RGCY 7 cut(s) 131, 199, 386, 593, 772, 799, 883
CviKI_1 RGCY 7 cut(s) 131, 199, 386, 593, 772, 799, 883
DdeI CTNAG 1 cut(s) 543
DpnI GATC 5 cut(s) 230, 423, 483, 787, 848
DpnII GATC 5 cut(s) 228, 421, 481, 785, 846
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
Eco31I GGTCTC 1 cut(s) 62
Eco47I GGWCC 1 cut(s) 390
Eco57I CTGAAG 3 cut(s) 48, 147, 171
EcoRII CCWGG 1 cut(s) 392
EcoT22I ATGCAT 1 cut(s) 10
Esp3I CGTCTC 1 cut(s) 608
FaeI CATG 6 cut(s) 16, 140, 235, 251, 737, 992
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FaqI GGGAC 2 cut(s) 639, 980
FatI CATG 6 cut(s) 12, 136, 231, 247, 733, 988
FauNDI CATATG 1 cut(s) 64
FokI GGATG 2 cut(s) 17, 173
FspBI CTAG 3 cut(s) 335, 377, 432
GlaI GCGC 2 cut(s) 353, 632
GsuI CTGGAG 1 cut(s) 556
HaeII RGCGCY 1 cut(s) 634
HaeIII GGCC 1 cut(s) 593
HapII CCGG 1 cut(s) 425
HgaI GACGC 1 cut(s) 625
HhaI GCGC 2 cut(s) 354, 633
Hin1I GRCGYC 1 cut(s) 617
Hin1II CATG 6 cut(s) 16, 140, 235, 251, 737, 992
Hin6I GCGC 2 cut(s) 352, 631
HinP1I GCGC 2 cut(s) 352, 631
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HinfI GANTC 3 cut(s) 146, 812, 978
HpaI GTTAAC 1 cut(s) 604
HpaII CCGG 1 cut(s) 425
HphI GGTGA 2 cut(s) 322, 575
Hpy166II GTNNAC 4 cut(s) 100, 175, 577, 604
Hpy188I TCNGA 4 cut(s) 151, 421, 817, 983
Hpy188III TCNNGA 9 cut(s) 224, 232, 335, 377, 425, 445, 743, 887, 989
Hpy8I GTNNAC 4 cut(s) 100, 175, 577, 604
HpyAV CCTTC 1 cut(s) 731
HpyCH4III ACNGT 2 cut(s) 172, 277
HpyCH4IV ACGT 2 cut(s) 292, 698
HpyCH4V TGCA 7 cut(s) 8, 100, 281, 644, 662, 759, 1038
HpyF10VI GCNNNNNNNGC 1 cut(s) 872
HpyF3I CTNAG 1 cut(s) 543
HpySE526I ACGT 2 cut(s) 292, 698
Hsp92I GRCGYC 1 cut(s) 617
Hsp92II CATG 6 cut(s) 16, 140, 235, 251, 737, 992
HspAI GCGC 2 cut(s) 352, 631
Kpn2I TCCGGA 1 cut(s) 424
KspAI GTTAAC 1 cut(s) 604
Kzo9I GATC 5 cut(s) 228, 421, 481, 785, 846
LmnI GCTCC 2 cut(s) 532, 547
LweI GCATC 4 cut(s) 13, 746, 853, 864
MaeI CTAG 3 cut(s) 335, 377, 432
MaeII ACGT 2 cut(s) 292, 698
MaeIII GTNAC 2 cut(s) 121, 899
MalI GATC 5 cut(s) 230, 423, 483, 787, 848
MboI GATC 5 cut(s) 228, 421, 481, 785, 846
MboII GAAGA 6 cut(s) 41, 164, 222, 225, 460, 856
MfeI CAATTG 1 cut(s) 639
MhlI GDGCHC 2 cut(s) 102, 1029
MluCI AATT 5 cut(s) 202, 236, 473, 639, 1030
MlyI GAGTC 2 cut(s) 821, 987
MmeI TCCRAC 1 cut(s) 1006
MnlI CCTC 9 cut(s) 61, 151, 179, 209, 376, 538, 705, 831, 956
Mph1103I ATGCAT 1 cut(s) 10
MroI TCCGGA 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 217
MseI TTAA 2 cut(s) 240, 603
MslI CAYNNNNRTG 2 cut(s) 50, 561
MspI CCGG 1 cut(s) 425
MspR9I CCNGG 1 cut(s) 394
MunI CAATTG 1 cut(s) 639
MvaI CCWGG 1 cut(s) 394
MvnI CGCG 1 cut(s) 354
MwoI GCNNNNNNNGC 1 cut(s) 872
NdeI CATATG 1 cut(s) 64
NdeII GATC 5 cut(s) 228, 421, 481, 785, 846
NlaIII CATG 6 cut(s) 16, 140, 235, 251, 737, 992
NlaIV GGNNCC 3 cut(s) 264, 716, 747
NmuCI GTSAC 1 cut(s) 121
NsiI ATGCAT 1 cut(s) 10
PagI TCATGA 2 cut(s) 231, 988
PdmI GAANNNNTTC 1 cut(s) 217
PfeI GAWTC 1 cut(s) 146
PflMI CCANNNNNTGG 1 cut(s) 414
PfoI TCCNGGA 1 cut(s) 392
PleI GAGTC 2 cut(s) 820, 986
PpsI GAGTC 2 cut(s) 820, 986
Psp1406I AACGTT 1 cut(s) 698
Psp6I CCWGG 1 cut(s) 392
PspGI CCWGG 1 cut(s) 392
PspN4I GGNNCC 3 cut(s) 264, 716, 747
PspPI GGNCC 2 cut(s) 390, 591
PstNI CAGNNNCTG 1 cut(s) 149
RseI CAYNNNNRTG 2 cut(s) 50, 561
SaqAI TTAA 2 cut(s) 240, 603
Sau3AI GATC 5 cut(s) 228, 421, 481, 785, 846
Sau96I GGNCC 2 cut(s) 390, 591
SchI GAGTC 2 cut(s) 821, 987
ScrFI CCNGG 1 cut(s) 394
SduI GDGCHC 2 cut(s) 102, 1029
SfaNI GCATC 4 cut(s) 13, 746, 853, 864
SinI GGWCC 1 cut(s) 390
SmiMI CAYNNNNRTG 2 cut(s) 50, 561
SmlI CTYRAG 2 cut(s) 224, 705
SmoI CTYRAG 2 cut(s) 224, 705
Sse9I AATT 5 cut(s) 202, 236, 473, 639, 1030
SspMI CTAG 3 cut(s) 335, 377, 432
StyD4I CCNGG 1 cut(s) 392
TaaI ACNGT 2 cut(s) 172, 277
TaiI ACGT 2 cut(s) 295, 701
TaqI TCGA 1 cut(s) 511
TasI AATT 5 cut(s) 202, 236, 473, 639, 1030
TfiI GAWTC 1 cut(s) 146
Tru1I TTAA 2 cut(s) 240, 603
Tru9I TTAA 2 cut(s) 240, 603
TscAI CASTG 3 cut(s) 177, 264, 856
TseFI GTSAC 1 cut(s) 121
Tsp45I GTSAC 1 cut(s) 121
TspDTI ATGAA 4 cut(s) 248, 689, 750, 977
TspGWI ACGGA 2 cut(s) 479, 1034
TspRI CASTG 3 cut(s) 177, 264, 856
Van91I CCANNNNNTGG 1 cut(s) 414
VneI GTGCAC 1 cut(s) 98
VpaK11BI GGWCC 1 cut(s) 390
XapI RAATTY 1 cut(s) 236
XbaI TCTAGA 2 cut(s) 334, 376
XcmI CCANNNNNNNNNTGG 1 cut(s) 326
XmnI GAANNNNTTC 1 cut(s) 217
XspI CTAG 3 cut(s) 335, 377, 432
Zsp2I ATGCAT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.