Rmu_sc0001083.1_g000024

Mitochondrial fission protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001083.1
OG0016966
Physical Location & Seq
Reverse (-)
145888 .. 150912
5025 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1461 bp
atgttgaacaagatttatgttaaatgcactattgggtgcgatcatactagcaataatgcaccggatcccatcaaaactttgaaattaagcttatttgggcagagcagtattaggatcactactagaattatgctcatagacatcacggcaattaaatcgattgccctaactattcgactagcacccaacctccctctcgtcctcttcgttctccgcctctggccaaaaccctcttcttcattctctgccttagattaccctaactatttgactagcacccaacctccccctcgtcctcttcgttctgcgcctctgaccaaaaccgtttcttcgttattagcctccaaccaaaacacgaccctcctcgtcctccttcttttccatctcggaccagctcctctgctcgacgatggaatgccgcatctcttctctctctctctctctctttccttctctctcctgcaaaactggtggctctggttatctggacttccagctatccacctctggttatccattttcgctggtgagtcggtgacattgctggtaagtcaaatttctggaattctgggttttagtcttccaatcgaatgtctgactctcacaatctcacaatttccaggtccacaatttcaatcgaaggctataactcttcctcttgctgtgctgctcgacgatggaatgccgcatctcttctctctctctctctctctttccttctctctcctgcaaaactggtggctctggttatctggacttccagctatccacctctggttatccatattcgctggtgagtcggtgacattgctggtaagtcaaatttctggaattctaggttttagtcttccaatggaatgtctgactctcacaatctcacaatttccaggtccacaatttcaatcgaaggctatatgtaaagccgcaaagcagaaattttgggttcgaagcttggtgattcttcaacttcgaagcttgatgattcttcaacctcgaagcttggtgattcttcaagctcgatttatgggttttggattaattgaagtatcgagctcgtggtcaactggtcttgctcttggagtcttggttcgattcagaaatcttcaagctcaggttctcaccattggatctctttatcaggatgattctgctgcacttaggagtgttgcttctgcctggcacgctgaactgacaccgctcccgaagcccttgcttgtagtcaatataggaggcccttctggacgggccttgtcgactccatgtgggtatggggagatgccgggtccctcgatcgtcgtcttgttcgtagttggggagatcgctgtggtggtttgtgcagtgaggaaaagggatcgtagattgtggcggtggtcgagtcttggtttgtccctcgcgggtttgatcggcggaagagattcgtgttactcggtcaaaggttgctggggggtgacgggatcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mito_fiss_Elm1

Protein Analysis

486

Amino Acids

52.95

Weight (kDa)

9.66

Isoelectric Point (pI)

55.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_fiss_Elm1 PF06258 371 - 415 2.5e-06 Mitochondrial fission ELM1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1198
AccI GTMKAC 1 cut(s) 1253
AccII CGCG 1 cut(s) 1394
AciI CCGC 8 cut(s) 214, 419, 686, 924, 1196, 1366, 1394, 1407
AclWI GGATC 5 cut(s) 59, 72, 122, 1135, 1359
AcoI YGGCCR 1 cut(s) 221
AcsI RAATTY 5 cut(s) 555, 564, 822, 831, 935
AgsI TTSAA 9 cut(s) 7, 82, 635, 902, 965, 989, 1013, 1043, 1106
AjnI CCWGG 3 cut(s) 619, 886, 1175
AjuI GAANNNNNNNTTGG 2 cut(s) 217, 249
AloI GAACNNNNNNTCC 2 cut(s) 1071, 1103
Alw21I GWGCWC 1 cut(s) 1055
AlwI GGATC 5 cut(s) 59, 72, 122, 1135, 1359
AoxI GGCC 3 cut(s) 221, 1231, 1245
ApeKI GCWGC 2 cut(s) 667, 1151
ApoI RAATTY 5 cut(s) 555, 564, 822, 831, 935
AseI ATTAAT 1 cut(s) 1037
Asp700I GAANNNNTTC 1 cut(s) 1414
AspLEI GCGC 1 cut(s) 310
AspS9I GGNCC 6 cut(s) 389, 623, 890, 1232, 1245, 1283
AsuC2I CCSGG 1 cut(s) 1281
AsuHPI GGTGA 8 cut(s) 539, 547, 806, 814, 967, 1015, 1111, 1459
AsuII TTCGAA 2 cut(s) 946, 970
AvaII GGWCC 4 cut(s) 389, 623, 890, 1283
BalI TGGCCA 1 cut(s) 223
BamHI GGATCC 1 cut(s) 64
BanII GRGCYC 1 cut(s) 1055
BauI CACGAG 1 cut(s) 1054
BbsI GAAGAC 2 cut(s) 572, 839
Bbv12I GWGCWC 1 cut(s) 1055
BbvI GCAGC 2 cut(s) 654, 1138
BccI CCATC 4 cut(s) 77, 390, 404, 671
BceAI ACGGC 1 cut(s) 162
BcgI CGANNNNNNTGC 4 cut(s) 138, 172, 1192, 1226
BciT130I CCWGG 3 cut(s) 621, 888, 1177
BcnI CCSGG 1 cut(s) 1281
BfaI CTAG 5 cut(s) 48, 123, 179, 273, 836
BisI GCNGC 5 cut(s) 419, 668, 686, 924, 1152
BlsI GCNGC 5 cut(s) 420, 669, 687, 925, 1153
Bme1390I CCNGG 4 cut(s) 621, 888, 1177, 1281
Bme18I GGWCC 4 cut(s) 389, 623, 890, 1283
BmgT120I GGNCC 6 cut(s) 389, 623, 890, 1232, 1245, 1283
BmiI GGNNCC 3 cut(s) 66, 1284, 1285
BmrFI CCNGG 4 cut(s) 621, 888, 1177, 1281
BmsI GCATC 3 cut(s) 430, 697, 1266
BpiI GAAGAC 2 cut(s) 572, 839
Bpu10I CCTNAGC 1 cut(s) 1110
Bpu14I TTCGAA 2 cut(s) 946, 970
BpuMI CCSGG 1 cut(s) 1281
Bsa29I ATCGAT 1 cut(s) 158
BsaWI WCCGGW 1 cut(s) 61
BsaXI ACNNNNNCTCC 6 cut(s) 174, 204, 268, 298, 1071, 1101
Bse1I ACTGG 3 cut(s) 473, 740, 1069
Bse3DI GCAATG 2 cut(s) 539, 806
BseBI CCWGG 3 cut(s) 621, 888, 1177
BseCI ATCGAT 1 cut(s) 158
BseGI GGATG 1 cut(s) 1147
BseMI GCAATG 2 cut(s) 539, 806
BseMII CTCAG 1 cut(s) 1124
BseNI ACTGG 3 cut(s) 473, 740, 1069
BseRI GAGGAG 2 cut(s) 353, 387
BseXI GCAGC 2 cut(s) 654, 1138
BseYI CCCAGC 1 cut(s) 1440
BsgI GTGCAG 2 cut(s) 1137, 1356
Bsh1236I CGCG 1 cut(s) 1394
Bsh1285I CGRYCG 1 cut(s) 1293
BshFI GGCC 3 cut(s) 223, 1233, 1247
BshVI ATCGAT 1 cut(s) 158
BsiEI CGRYCG 1 cut(s) 1293
BsiHKAI GWGCWC 1 cut(s) 1055
BsiSI CCGG 2 cut(s) 62, 1280
BslFI GGGAC 2 cut(s) 1269, 1372
BsmFI GGGAC 2 cut(s) 1269, 1372
BsmI GAATGC 2 cut(s) 420, 687
BsnI GGCC 3 cut(s) 223, 1233, 1247
Bsp119I TTCGAA 2 cut(s) 946, 970
Bsp1286I GDGCHC 1 cut(s) 1055
Bsp143I GATC 9 cut(s) 40, 64, 114, 1127, 1290, 1317, 1351, 1401, 1454
BspACI CCGC 8 cut(s) 214, 419, 686, 924, 1196, 1366, 1394, 1407
BspANI GGCC 3 cut(s) 223, 1233, 1247
BspCNI CTCAG 1 cut(s) 1123
BspDI ATCGAT 1 cut(s) 158
BspFNI CGCG 1 cut(s) 1394
BspHI TCATGA 1 cut(s) 1457
BspLI GGNNCC 3 cut(s) 66, 1284, 1285
BspPI GGATC 5 cut(s) 59, 72, 122, 1135, 1359
BspT104I TTCGAA 2 cut(s) 946, 970
BsrBI CCGCTC 1 cut(s) 1198
BsrDI GCAATG 2 cut(s) 539, 806
BsrI ACTGG 3 cut(s) 473, 740, 1069
BssMI GATC 9 cut(s) 40, 64, 114, 1127, 1290, 1317, 1351, 1401, 1454
BssSI CACGAG 1 cut(s) 1054
Bst2BI CACGAG 1 cut(s) 1054
Bst2UI CCWGG 3 cut(s) 621, 888, 1177
Bst4CI ACNGT 1 cut(s) 325
Bst6I CTCTTC 7 cut(s) 209, 238, 303, 431, 657, 698, 1405
BstBI TTCGAA 2 cut(s) 946, 970
BstC8I GCNNGC 1 cut(s) 1182
BstDEI CTNAG 3 cut(s) 250, 1110, 1157
BstF5I GGATG 1 cut(s) 1147
BstFNI CGCG 1 cut(s) 1394
BstHHI GCGC 1 cut(s) 310
BstKTI GATC 9 cut(s) 43, 67, 117, 1130, 1293, 1320, 1354, 1404, 1457
BstMBI GATC 9 cut(s) 40, 64, 114, 1127, 1290, 1317, 1351, 1401, 1454
BstMCI CGRYCG 1 cut(s) 1293
BstMWI GCNNNNNNNGC 2 cut(s) 1181, 1204
BstNI CCWGG 3 cut(s) 621, 888, 1177
BstSCI CCNGG 4 cut(s) 619, 886, 1175, 1279
BstUI CGCG 1 cut(s) 1394
BstV1I GCAGC 2 cut(s) 654, 1138
BstV2I GAAGAC 2 cut(s) 572, 839
BstX2I RGATCY 2 cut(s) 64, 1127
BstYI RGATCY 2 cut(s) 64, 1127
Bsu15I ATCGAT 1 cut(s) 158
BsuRI GGCC 3 cut(s) 223, 1233, 1247
BsuTUI ATCGAT 1 cut(s) 158
BtsCI GGATG 1 cut(s) 1147
BtsI GCAGTG 1 cut(s) 1344
BtsIMutI CAGTG 1 cut(s) 1344
Cac8I GCNNGC 1 cut(s) 1182
CciI TCATGA 1 cut(s) 1457
CfoI GCGC 1 cut(s) 310
Cfr13I GGNCC 6 cut(s) 389, 623, 890, 1232, 1245, 1283
ClaI ATCGAT 1 cut(s) 158
CspCI CAANNNNNGTGG 4 cut(s) 452, 487, 719, 754
CviAII CATG 2 cut(s) 1260, 1458
DdeI CTNAG 3 cut(s) 250, 1110, 1157
DpnI GATC 9 cut(s) 42, 66, 116, 1129, 1292, 1319, 1353, 1403, 1456
DpnII GATC 9 cut(s) 40, 64, 114, 1127, 1290, 1317, 1351, 1401, 1454
EaeI YGGCCR 1 cut(s) 221
Eam1104I CTCTTC 7 cut(s) 209, 238, 303, 431, 657, 698, 1405
EarI CTCTTC 7 cut(s) 209, 238, 303, 431, 657, 698, 1405
EciI GGCGGA 2 cut(s) 203, 1422
Ecl136II GAGCTC 1 cut(s) 1053
Eco24I GRGCYC 1 cut(s) 1055
Eco47I GGWCC 4 cut(s) 389, 623, 890, 1283
Eco53kI GAGCTC 1 cut(s) 1053
EcoICRI GAGCTC 1 cut(s) 1053
EcoO109I RGGNCCY 2 cut(s) 1232, 1283
EcoRI GAATTC 2 cut(s) 564, 831
EcoRII CCWGG 3 cut(s) 619, 886, 1175
EcoT38I GRGCYC 1 cut(s) 1055
FaeI CATG 2 cut(s) 1263, 1461
FaqI GGGAC 2 cut(s) 1269, 1372
FatI CATG 2 cut(s) 1259, 1457
FauI CCCGC 1 cut(s) 1387
FblI GTMKAC 1 cut(s) 1253
Fnu4HI GCNGC 5 cut(s) 419, 668, 686, 924, 1152
FokI GGATG 1 cut(s) 1154
FriOI GRGCYC 1 cut(s) 1055
Fsp4HI GCNGC 5 cut(s) 419, 668, 686, 924, 1152
FspBI CTAG 5 cut(s) 48, 123, 179, 273, 836
GlaI GCGC 1 cut(s) 309
GluI GCNGC 5 cut(s) 419, 668, 686, 924, 1152
GsaI CCCAGC 1 cut(s) 1444
HaeIII GGCC 3 cut(s) 223, 1233, 1247
HapII CCGG 2 cut(s) 62, 1280
HhaI GCGC 1 cut(s) 310
Hin1II CATG 2 cut(s) 1263, 1461
Hin6I GCGC 1 cut(s) 308
HinP1I GCGC 1 cut(s) 308
HincII GTYRAC 2 cut(s) 1062, 1254
HindII GTYRAC 2 cut(s) 1062, 1254
HindIII AAGCTT 4 cut(s) 88, 949, 973, 997
HpaII CCGG 2 cut(s) 62, 1280
HphI GGTGA 8 cut(s) 539, 547, 806, 814, 967, 1015, 1111, 1459
Hpy166II GTNNAC 4 cut(s) 626, 893, 1062, 1254
Hpy188I TCNGA 5 cut(s) 315, 389, 597, 864, 1097
Hpy188III TCNNGA 8 cut(s) 486, 561, 753, 828, 1139, 1201, 1239, 1458
Hpy8I GTNNAC 4 cut(s) 626, 893, 1062, 1254
Hpy99I CGWCG 3 cut(s) 410, 677, 1298
HpyAV CCTTC 6 cut(s) 383, 460, 634, 727, 901, 1245
HpyCH4III ACNGT 1 cut(s) 325
HpyCH4V TGCA 6 cut(s) 27, 59, 463, 730, 1154, 1337
HpyF10VI GCNNNNNNNGC 2 cut(s) 1181, 1204
HpyF3I CTNAG 3 cut(s) 250, 1110, 1157
Hsp92II CATG 2 cut(s) 1263, 1461
HspAI GCGC 1 cut(s) 308
KflI GGGWCCC 1 cut(s) 1283
Kzo9I GATC 9 cut(s) 40, 64, 114, 1127, 1290, 1317, 1351, 1401, 1454
LmnI GCTCC 2 cut(s) 400, 1203
Lsp1109I GCAGC 2 cut(s) 654, 1138
LweI GCATC 3 cut(s) 430, 697, 1266
MaeI CTAG 5 cut(s) 48, 123, 179, 273, 836
MaeIII GTNAC 4 cut(s) 535, 802, 1421, 1447
MalI GATC 9 cut(s) 42, 66, 116, 1129, 1292, 1319, 1353, 1403, 1456
MbiI CCGCTC 1 cut(s) 1198
MboI GATC 9 cut(s) 40, 64, 114, 1127, 1290, 1317, 1351, 1401, 1454
MflI RGATCY 2 cut(s) 64, 1127
MhlI GDGCHC 1 cut(s) 1055
MlsI TGGCCA 1 cut(s) 223
MluNI TGGCCA 1 cut(s) 223
MlyI GAGTC 7 cut(s) 539, 592, 806, 859, 1089, 1249, 1384
MmeI TCCRAC 1 cut(s) 369
Mox20I TGGCCA 1 cut(s) 223
MroXI GAANNNNTTC 1 cut(s) 1414
MscI TGGCCA 1 cut(s) 223
MseI TTAA 4 cut(s) 21, 86, 153, 1037
Msp20I TGGCCA 1 cut(s) 223
MspI CCGG 2 cut(s) 62, 1280
MspR9I CCNGG 4 cut(s) 621, 888, 1177, 1281
Mva1269I GAATGC 2 cut(s) 420, 687
MvaI CCWGG 3 cut(s) 621, 888, 1177
MvnI CGCG 1 cut(s) 1394
MwoI GCNNNNNNNGC 2 cut(s) 1181, 1204
NciI CCSGG 1 cut(s) 1281
NdeII GATC 9 cut(s) 40, 64, 114, 1127, 1290, 1317, 1351, 1401, 1454
NlaIII CATG 2 cut(s) 1263, 1461
NlaIV GGNNCC 3 cut(s) 66, 1284, 1285
NmuCI GTSAC 3 cut(s) 535, 802, 1447
NspV TTCGAA 2 cut(s) 946, 970
PagI TCATGA 1 cut(s) 1457
PcsI WCGNNNNNNNCGW 3 cut(s) 204, 298, 1302
PctI GAATGC 2 cut(s) 420, 687
PdmI GAANNNNTTC 1 cut(s) 1414
PfeI GAWTC 6 cut(s) 958, 982, 1006, 1092, 1145, 1415
PkrI GCNGC 5 cut(s) 420, 669, 687, 925, 1153
Ple19I CGATCG 1 cut(s) 1293
PleI GAGTC 7 cut(s) 538, 592, 805, 859, 1088, 1249, 1383
PpsI GAGTC 7 cut(s) 538, 592, 805, 859, 1088, 1249, 1383
PpuMI RGGWCCY 1 cut(s) 1283
PshBI ATTAAT 1 cut(s) 1037
Psp124BI GAGCTC 1 cut(s) 1055
Psp5II RGGWCCY 1 cut(s) 1283
Psp6I CCWGG 3 cut(s) 619, 886, 1175
PspFI CCCAGC 1 cut(s) 1440
PspGI CCWGG 3 cut(s) 619, 886, 1175
PspN4I GGNNCC 3 cut(s) 66, 1284, 1285
PspPI GGNCC 6 cut(s) 389, 623, 890, 1232, 1245, 1283
PspPPI RGGWCCY 1 cut(s) 1283
PsuI RGATCY 2 cut(s) 64, 1127
PvuI CGATCG 1 cut(s) 1293
SacI GAGCTC 1 cut(s) 1055
SalI GTCGAC 1 cut(s) 1252
SaqAI TTAA 4 cut(s) 21, 86, 153, 1037
SatI GCNGC 5 cut(s) 419, 668, 686, 924, 1152
Sau3AI GATC 9 cut(s) 40, 64, 114, 1127, 1290, 1317, 1351, 1401, 1454
Sau96I GGNCC 6 cut(s) 389, 623, 890, 1232, 1245, 1283
SchI GAGTC 7 cut(s) 539, 592, 806, 859, 1089, 1249, 1384
ScrFI CCNGG 4 cut(s) 621, 888, 1177, 1281
SduI GDGCHC 1 cut(s) 1055
SfaNI GCATC 3 cut(s) 430, 697, 1266
SfuI TTCGAA 2 cut(s) 946, 970
SinI GGWCC 4 cut(s) 389, 623, 890, 1283
SsiI CCGC 8 cut(s) 214, 419, 686, 924, 1196, 1366, 1394, 1407
SspMI CTAG 5 cut(s) 48, 123, 179, 273, 836
SstI GAGCTC 1 cut(s) 1055
StyD4I CCNGG 4 cut(s) 619, 886, 1175, 1279
TaaI ACNGT 1 cut(s) 325
TaqII GACCGA 1 cut(s) 1417
TauI GCSGC 3 cut(s) 421, 688, 926
TfiI GAWTC 6 cut(s) 958, 982, 1006, 1092, 1145, 1415
Tru1I TTAA 4 cut(s) 21, 86, 153, 1037
Tru9I TTAA 4 cut(s) 21, 86, 153, 1037
TscAI CASTG 1 cut(s) 1344
TseFI GTSAC 3 cut(s) 535, 802, 1447
TseI GCWGC 2 cut(s) 667, 1151
Tsp45I GTSAC 3 cut(s) 535, 802, 1447
TspDTI ATGAA 1 cut(s) 228
TspRI CASTG 1 cut(s) 1344
VpaK11BI GGWCC 4 cut(s) 389, 623, 890, 1283
VspI ATTAAT 1 cut(s) 1037
XapI RAATTY 5 cut(s) 555, 564, 822, 831, 935
XmiI GTMKAC 1 cut(s) 1253
XmnI GAANNNNTTC 1 cut(s) 1414
XspI CTAG 5 cut(s) 48, 123, 179, 273, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.